LOCUS       DAZKCN010000001        80134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000001 DAZKCN010000000
VERSION     DAZKCN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.001"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..730
                     /locus_tag="ACG8K3_000001"
     CDS             98..730
                     /locus_tag="ACG8K3_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9143918.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(864..989)
                     /locus_tag="ACG8K3_000002"
     CDS             complement(864..989)
                     /locus_tag="ACG8K3_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP9143919.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1123..2016)
                     /locus_tag="ACG8K3_000003"
     CDS             complement(1123..2016)
                     /locus_tag="ACG8K3_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP9143920.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2198..3124)
                     /locus_tag="ACG8K3_000004"
     CDS             complement(2198..3124)
                     /locus_tag="ACG8K3_000004"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP9143921.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3209..3970)
                     /gene="pflA"
                     /locus_tag="ACG8K3_000005"
     CDS             complement(3209..3970)
                     /gene="pflA"
                     /locus_tag="ACG8K3_000005"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP9143922.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4214..6445)
                     /gene="pflB"
                     /locus_tag="ACG8K3_000006"
     CDS             complement(4214..6445)
                     /gene="pflB"
                     /locus_tag="ACG8K3_000006"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9143923.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6722..9172)
                     /gene="parC"
                     /locus_tag="ACG8K3_000007"
     CDS             complement(6722..9172)
                     /gene="parC"
                     /locus_tag="ACG8K3_000007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9143924.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9184..11229)
                     /gene="parE"
                     /locus_tag="ACG8K3_000008"
     CDS             complement(9184..11229)
                     /gene="parE"
                     /locus_tag="ACG8K3_000008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9143925.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11395..11829)
                     /locus_tag="ACG8K3_000009"
     CDS             complement(11395..11829)
                     /locus_tag="ACG8K3_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9143926.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11956..12594
                     /gene="plsY"
                     /locus_tag="ACG8K3_000010"
     CDS             11956..12594
                     /gene="plsY"
                     /locus_tag="ACG8K3_000010"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9143927.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12726..13601)
                     /locus_tag="ACG8K3_000011"
     CDS             complement(12726..13601)
                     /locus_tag="ACG8K3_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9143928.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13683..14474)
                     /gene="codY"
                     /locus_tag="ACG8K3_000012"
     CDS             complement(13683..14474)
                     /gene="codY"
                     /locus_tag="ACG8K3_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP9143929.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14492..15892)
                     /gene="hslU"
                     /locus_tag="ACG8K3_000013"
     CDS             complement(14492..15892)
                     /gene="hslU"
                     /locus_tag="ACG8K3_000013"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP9143930.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15906..16454)
                     /gene="hslV"
                     /locus_tag="ACG8K3_000014"
     CDS             complement(15906..16454)
                     /gene="hslV"
                     /locus_tag="ACG8K3_000014"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP9143931.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16491..17396)
                     /gene="xerC"
                     /locus_tag="ACG8K3_000015"
     CDS             complement(16491..17396)
                     /gene="xerC"
                     /locus_tag="ACG8K3_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9143932.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17594..18970)
                     /gene="trmFO"
                     /locus_tag="ACG8K3_000016"
     CDS             complement(17594..18970)
                     /gene="trmFO"
                     /locus_tag="ACG8K3_000016"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP9143933.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18939..21017)
                     /gene="topA"
                     /locus_tag="ACG8K3_000017"
     CDS             complement(18939..21017)
                     /gene="topA"
                     /locus_tag="ACG8K3_000017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9143934.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21139..21996)
                     /gene="dprA"
                     /locus_tag="ACG8K3_000018"
     CDS             complement(21139..21996)
                     /gene="dprA"
                     /locus_tag="ACG8K3_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9143935.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(22052..22819)
                     /locus_tag="ACG8K3_000019"
     CDS             complement(22052..22819)
                     /locus_tag="ACG8K3_000019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9143936.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22812..23672)
                     /gene="ylqF"
                     /locus_tag="ACG8K3_000020"
     CDS             complement(22812..23672)
                     /gene="ylqF"
                     /locus_tag="ACG8K3_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP9143937.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23932..24330
                     /locus_tag="ACG8K3_000021"
     CDS             23932..24330
                     /locus_tag="ACG8K3_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9143938.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24567..25109
                     /locus_tag="ACG8K3_000022"
     CDS             24567..25109
                     /locus_tag="ACG8K3_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9143939.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25192..25743)
                     /gene="lepB"
                     /locus_tag="ACG8K3_000023"
     CDS             complement(25192..25743)
                     /gene="lepB"
                     /locus_tag="ACG8K3_000023"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9143940.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25895..26122)
                     /locus_tag="ACG8K3_000024"
     CDS             complement(25895..26122)
                     /locus_tag="ACG8K3_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP9143941.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26119..26637)
                     /gene="msrA"
                     /locus_tag="ACG8K3_000025"
     CDS             complement(26119..26637)
                     /gene="msrA"
                     /locus_tag="ACG8K3_000025"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9143942.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26657..27370)
                     /locus_tag="ACG8K3_000026"
     CDS             complement(26657..27370)
                     /locus_tag="ACG8K3_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP9143943.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27373..28269)
                     /locus_tag="ACG8K3_000027"
     CDS             complement(27373..28269)
                     /locus_tag="ACG8K3_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9143944.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28427..29269)
                     /locus_tag="ACG8K3_000028"
     CDS             complement(28427..29269)
                     /locus_tag="ACG8K3_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9143945.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29401..30054
                     /locus_tag="ACG8K3_000029"
     CDS             29401..30054
                     /locus_tag="ACG8K3_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9143946.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30117..30644)
                     /locus_tag="ACG8K3_000030"
     CDS             complement(30117..30644)
                     /locus_tag="ACG8K3_000030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9143947.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30664..31611)
                     /locus_tag="ACG8K3_000031"
     CDS             complement(30664..31611)
                     /locus_tag="ACG8K3_000031"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9143948.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31623..33506)
                     /locus_tag="ACG8K3_000032"
     CDS             complement(31623..33506)
                     /locus_tag="ACG8K3_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9143949.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33669..35747)
                     /locus_tag="ACG8K3_000033"
     CDS             complement(33669..35747)
                     /locus_tag="ACG8K3_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9143950.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35760..36035)
                     /locus_tag="ACG8K3_000034"
     CDS             complement(35760..36035)
                     /locus_tag="ACG8K3_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9143951.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36397..36852)
                     /locus_tag="ACG8K3_000035"
     CDS             complement(36397..36852)
                     /locus_tag="ACG8K3_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9143952.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(37006..38214)
                     /locus_tag="ACG8K3_000036"
     CDS             complement(37006..38214)
                     /locus_tag="ACG8K3_000036"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP9143953.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38326..38658)
                     /locus_tag="ACG8K3_000037"
     CDS             complement(38326..38658)
                     /locus_tag="ACG8K3_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9143954.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38806..39681
                     /locus_tag="ACG8K3_000038"
     CDS             38806..39681
                     /locus_tag="ACG8K3_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9143955.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39763..40344)
                     /locus_tag="ACG8K3_000039"
     CDS             complement(39763..40344)
                     /locus_tag="ACG8K3_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP9143956.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40350..41609)
                     /locus_tag="ACG8K3_000040"
     CDS             complement(40350..41609)
                     /locus_tag="ACG8K3_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9143957.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41611..42657)
                     /locus_tag="ACG8K3_000041"
     CDS             complement(41611..42657)
                     /locus_tag="ACG8K3_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9143958.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42892..43167)
                     /locus_tag="ACG8K3_000042"
     CDS             complement(42892..43167)
                     /locus_tag="ACG8K3_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP9143959.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43410..44720)
                     /gene="der"
                     /locus_tag="ACG8K3_000043"
     CDS             complement(43410..44720)
                     /gene="der"
                     /locus_tag="ACG8K3_000043"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9143960.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44902..46131)
                     /gene="rpsA"
                     /locus_tag="ACG8K3_000044"
     CDS             complement(44902..46131)
                     /gene="rpsA"
                     /locus_tag="ACG8K3_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9143961.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46266..46946)
                     /gene="cmk"
                     /locus_tag="ACG8K3_000045"
     CDS             complement(46266..46946)
                     /gene="cmk"
                     /locus_tag="ACG8K3_000045"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9143962.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46995..47630)
                     /locus_tag="ACG8K3_000046"
     CDS             complement(46995..47630)
                     /locus_tag="ACG8K3_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP9143963.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47696..49099)
                     /locus_tag="ACG8K3_000047"
     CDS             complement(47696..49099)
                     /locus_tag="ACG8K3_000047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9143964.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(49086..50117)
                     /locus_tag="ACG8K3_000048"
     CDS             complement(49086..50117)
                     /locus_tag="ACG8K3_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9143965.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50148..50369
                     /locus_tag="ACG8K3_000049"
     CDS             50148..50369
                     /locus_tag="ACG8K3_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP9143966.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50534..51271)
                     /locus_tag="ACG8K3_000050"
     CDS             complement(50534..51271)
                     /locus_tag="ACG8K3_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP9143967.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51424..52764)
                     /locus_tag="ACG8K3_000051"
     CDS             complement(51424..52764)
                     /locus_tag="ACG8K3_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9143968.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(53001..54524)
                     /locus_tag="ACG8K3_000052"
     CDS             complement(53001..54524)
                     /locus_tag="ACG8K3_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9143969.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54902..55513)
                     /locus_tag="ACG8K3_000053"
     CDS             complement(54902..55513)
                     /locus_tag="ACG8K3_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9143970.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55636..55761)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55843..56559)
                     /locus_tag="ACG8K3_000054"
     CDS             complement(55843..56559)
                     /locus_tag="ACG8K3_000054"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9143971.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56565..57134)
                     /gene="scpB"
                     /locus_tag="ACG8K3_000055"
     CDS             complement(56565..57134)
                     /gene="scpB"
                     /locus_tag="ACG8K3_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP9143972.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57118..57888)
                     /locus_tag="ACG8K3_000056"
     CDS             complement(57118..57888)
                     /locus_tag="ACG8K3_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP9143973.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57885..58256)
                     /locus_tag="ACG8K3_000057"
     CDS             complement(57885..58256)
                     /locus_tag="ACG8K3_000057"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9143974.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58275..59162)
                     /gene="xerD"
                     /locus_tag="ACG8K3_000058"
     CDS             complement(58275..59162)
                     /gene="xerD"
                     /locus_tag="ACG8K3_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9143975.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59258..59731)
                     /locus_tag="ACG8K3_000059"
     CDS             complement(59258..59731)
                     /locus_tag="ACG8K3_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9143976.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59836..60699)
                     /locus_tag="ACG8K3_000060"
     CDS             complement(59836..60699)
                     /locus_tag="ACG8K3_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP9143977.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60895..62994)
                     /locus_tag="ACG8K3_000061"
     CDS             complement(60895..62994)
                     /locus_tag="ACG8K3_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP9143978.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63214..64323)
                     /gene="rpoD"
                     /locus_tag="ACG8K3_000062"
     CDS             complement(63214..64323)
                     /gene="rpoD"
                     /locus_tag="ACG8K3_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9143979.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64347..66188)
                     /gene="dnaG"
                     /locus_tag="ACG8K3_000063"
     CDS             complement(64347..66188)
                     /gene="dnaG"
                     /locus_tag="ACG8K3_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9143980.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66370..68685)
                     /locus_tag="ACG8K3_000064"
     CDS             complement(66370..68685)
                     /locus_tag="ACG8K3_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9143981.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68870..69730)
                     /locus_tag="ACG8K3_000065"
     CDS             complement(68870..69730)
                     /locus_tag="ACG8K3_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP9143982.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69789..70421)
                     /locus_tag="ACG8K3_000066"
     CDS             complement(69789..70421)
                     /locus_tag="ACG8K3_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9143983.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            70588..71349
                     /locus_tag="ACG8K3_000067"
     CDS             70588..71349
                     /locus_tag="ACG8K3_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9143984.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71375..73966
                     /gene="mprF"
                     /locus_tag="ACG8K3_000068"
     CDS             71375..73966
                     /gene="mprF"
                     /locus_tag="ACG8K3_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9143985.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74154..75341)
                     /gene="tuf"
                     /locus_tag="ACG8K3_000069"
     CDS             complement(74154..75341)
                     /gene="tuf"
                     /locus_tag="ACG8K3_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9143986.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75709..76065
                     /locus_tag="ACG8K3_000070"
     CDS             75709..76065
                     /locus_tag="ACG8K3_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9143987.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(76306..77310)
                     /locus_tag="ACG8K3_000071"
     CDS             complement(76306..77310)
                     /locus_tag="ACG8K3_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP9143988.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            77476..78327
                     /locus_tag="ACG8K3_000072"
     CDS             77476..78327
                     /locus_tag="ACG8K3_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9143989.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            78344..78589
                     /locus_tag="ACG8K3_000073"
     CDS             78344..78589
                     /locus_tag="ACG8K3_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9143990.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(78633..80078)
                     /locus_tag="ACG8K3_000074"
     CDS             complement(78633..80078)
                     /locus_tag="ACG8K3_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFP9143991.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIVVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KAKKPMAFYLKFLQRLTKRSWQLSHSFLSTV"
BASE COUNT        22031 a        16809 c        13749 g        27545 t
ORIGIN      
        1 acagttttta tttattattt tgattaaaaa acatcgacat atactcatca atttgttatt
       61 atactttata tacttatcaa tttaatttgg agatattatg gaaattcaac gtagaaccac
      121 taatttttta catttaggct ctttttttgc aactttgacg atcttatttt ttctatgtta
      181 ttcatacctt aagacctcac atgcgccttc tagtgcatta ggctggttgc ttttggatca
      241 ttctgatatc acagaagccg aagaaaatcc gttttttttg attttgtctt ctctttgcgg
      301 cttactgttt tctgtttatt tcggtgccta tttttttcat cgacatcctg gtacgttaaa
      361 cgaagcttca agcgtattga ttgcatttaa aacgttttct tttttagcaa ttctttttta
      421 ttcgtttaat cacaatacgc ttttcttctt tatcatgaat ggtttagtcg tgattttatc
      481 catttttact tattttattt tttcactcat ttttagccaa ttgaaatgtc cgctcttctt
      541 tcgattagtt cctgtcacct gttttattta cagcttgttt tccttttttg tccttgcttt
      601 aatgccactg accaaccctt cgacgatcca agtcgcagga aatctgttgg atatgctctt
      661 catcgtcagt ctaggtgtat ttatgtggca aagaaaaaac aaacaaaata aaaagcattt
      721 tgaagcctga ctattacgac ttcataaatg cttttttagt tttaaacact tcaatctaaa
      781 agaataaccc aagattggat aaattattaa tgactcgcca ttcgtttctt agtgattctg
      841 gattatcaaa tggacttcct aatctaatca gctagttctt cccataaaat gccatacttg
      901 actgaatcat aatattgatt atttaaaaat ctaacttttc ttatcactac ttcctttgtc
      961 attccacatt tctcaccatt tttttgcatt cctttgtttc cagaccatgt agtgaatcta
     1021 ataggtggta gataggcaaa atactcaaat aattctttta accataaagg aagagaaagg
     1081 ctcctcctgc aagagtcagt ataatttttg cttactgctc tcttaggttt tactcactaa
     1141 aactgttcca tattctttga tataagaaaa tgcatgcggt ataaatttct tgctcagctc
     1201 agaaaagatt tgtcgtttaa gaaaggccat ttcctccatt gtcaatgaag gatccaatgt
     1261 tggtaattca atctcaaaat tgtttttttc gacaataggt aattctaatg tgaatgattc
     1321 ttcttccttt atagtattcg tcacaagtag agactgatgg aaaatactag gccggcgatt
     1381 tttatattta ttcttacgta tcctgtcaac gataacttct atttgatcaa aaaagtacga
     1441 ttcatttacg tctccaataa gttcggctaa ttctttgaat gccttcgtta caggaagctg
     1501 attcttctgt acaaaaaagt aattagccaa agtaagcggg aagtgttcta tagaggaaag
     1561 tgtgataaaa cggtacttct catttcctgc agttgtcctc agcaattctg tctctcggct
     1621 gtttcttact ccataaaaat acgcttcttt ccgttcatct tcgaaacaat acgcccaaat
     1681 aacttctccc atcgctaatt tttgttcatc aacagagagt ctcttcaatt gttcagctat
     1741 ggtttcagca gccgaagtgg cttgttgcaa gtaatcttta ggatcaaata ttggaaagtt
     1801 caaatgatat cttcgttctt ccctcttgat taatcctgca tcgatcaata aatcaagaaa
     1861 atgggaaaat ttattttgcg aaaattcttt tttcaattct cttagaatcg tttcttgttc
     1921 tcgatgttca tatagataat cggtcaatga agaaaaaagc ggatcatcgg tcagtttttg
     1981 ataaaagttt tccttatttt ttgaataata gttcatatat gttctctttt ctatccgttt
     2041 tattttactt aaaaggcttt tacgtaaaaa ggaggaagaa caagagtcat aattgactgt
     2101 tattctcctc catttcttcg gcctttagta tgttctatgt ttctttttat atcaatcgct
     2161 tatccattca ttttgaatgc ttcatactac catttgatta gctaaataca gccgtcaatt
     2221 gaggtacgac ttgtttttta cgtgaaacga cacctttaag caaagcacgg ttgttggtca
     2281 aagttgtttg gaaagcttct tctacttttt cttttccttt accagcaaca agtaattctg
     2341 aatcactgtc taagatgttt gtgacaacta atacgaacag atcgtagcca tttgctttca
     2401 tttcatcttc cattgctgct tctagctctg cttgtcgatc catcacttca ttaaggtcca
     2461 ctgtattgac ttgagcgata cgaacgttgg aactgcccat tgggaaactt tttgcatcca
     2521 aatcaagaag aaccgcagct gatttatcac ttagatttgt tcctgctttc aacatttcaa
     2581 gtccataagt gtttagatcg acttcagcga ttgctgcaag ttcttttgca gctgcttcat
     2641 cttcaggcgt acatgttggt gatttgaaca acaatgtatc tgaaatgata gctgagagca
     2701 tgattcctgc gatttcttta ggaattgtta cttggttttc tttatatagt tttaaaataa
     2761 tcgtacttgt acatccaact ggttctgcac gatagtacaa tggattagct gtttcaaagt
     2821 ttgcaatacg atggtggtct actacagcta aaatatttac ttcgctgata tctgaaatac
     2881 tttgttggaa ttcattatga tccacaagca tgacatctgc attttctgct ttagctgact
     2941 ctactactcg tggtgcagat agctgaaagt ggtctaatgc gtattgtgtt tcttcattag
     3001 gtgtacctag agcaactgct tctgtttctt tacctaattc attttgcaag tgtgaaaacg
     3061 caatggctgc cccgattgca tctgtatctg gattttggtg accaaaaact aaaatttttg
     3121 acatgtcatt cgctcctttg tgatttctcc tatttaataa tagcaaaaaa acggaaagaa
     3181 gcaatactta tcttctttcc gatgcgacct atcgcgttaa atattttgta tactcttctg
     3241 tatgaagcaa tcgtttagca ttttcgacac gttcttttgt aggtggttcg atcccttcta
     3301 atggatattt caaaccaagt gtttcccact tgtattttcc catcgtatga taaggaagaa
     3361 tctctactct gtctacattt gacaaggttt gaataaagtt gtctagtcgg atcaaatatt
     3421 catcataatc acttcgctca ggaactagta catggcgaat ccaaaccggt ttgttgattt
     3481 ttgacaaata ttgagccatt tctaaaatat tttcattgtc ccaatgcgtt agttttttat
     3541 gctcttcatt atcgatgtgt ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt
     3601 cttgaaaacg tgaaaagaaa ggttcttcat acgtaaaagg ttttccgcat gtatccaatg
     3661 tagtatgcat cttccgctct tttgcttttt taaatagttc gatcaaaaaa tcgatctgca
     3721 atagaggttc tccaccacta acagtaatac cacctttatc tccccagtaa ggtctgaagc
     3781 gttctgcttt atctaaaagt tcatctgctg tgtattcttt ccctccgcca atattccaag
     3841 tatctggatt gtgacaaaat tggcaacgca tgcggcatcc ttgcataaac acgacaaagc
     3901 gcagtccagg tccgtctact gaaccgaaag tttcgataga atgaacatag ccgattgttt
     3961 tttcttccat ttaagttcac atcttttctt tgatttcatt ttaagtacaa gttagctgtc
     4021 gaagcgaagc tttctgaaca aaatttttcg caattgccgc ttcgttatgt gtaaaattta
     4081 cagggacgga acatacaagt cctgcccctg taaatcgttt ctttcaaata agcatactga
     4141 ttattcgatt ttgatgcgat ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt
     4201 tttcacgaac tttttacatt ctgtcgtgtg aagttctcat caagacatct aattgttgtt
     4261 ctcttgtcaa gtcacggaat ttaactgcat aaccagatac ccggatcgtc aagttcggat
     4321 atttcattgg attttccatt gcatcacgca ataaatcatt actgaataca ttgacattca
     4381 aatgataagc tccttttgag aagtatccat ctaagacatt acgcaagttt tcgattcttg
     4441 tatcttgttc ttttccaaga cctgaaggat tgattgtttg ggtatttgaa attccatcca
     4501 atgcacagcc gtaattcatt ttagctgttg agttcaggct agctaataac ccactttgtt
     4561 ccgcattgta acttggattt gctcctggcg caagtggttt tccagcttca cggccatcag
     4621 gagtatttcc tgtattttta ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg
     4681 ttggttttga gttgcggtaa gttttatgac gtttgatttg tcccatgaag tattccaaga
     4741 tccatgaaac taattcatct gcacggtcat catcatttcc gtatttaggg aagtcgccat
     4801 ccacttcaaa atcaactgct attccttttt cattacggat tgcttttact tttgcatatt
     4861 tgatcgctga caagctgtct gccgcatgag agatcccagc aatacctgta gcaaatgtgc
     4921 gttgcaagtc agaatccatc aaagccagct gagctgcttc ataagaatat ttatcatgca
     4981 tataatgaat gatgttcaac gtattgacat atagttctgc taaccaatcc atcatattca
     5041 tgaagttatg tttcacttct tcaaagtcta gtatttctga tgtgattgga cgatattctg
     5101 gtccaacttg ggcgccagtc acttcatcga ctccgccatt gatggcatat aatagtgttt
     5161 tagccaagtt agcacgagcg ccaaagaatt gcatgtcttt tccgacaact gttcctgata
     5221 cacagcaagc aatcgcacaa tcatcagaac cccattgttc acgaagctga ttatcatttt
     5281 cgaattgaat cgaagaactt tcgatagcga ttctgctagc atatgttctg aatccttctg
     5341 gaaggtgttc agaatacaaa acagtcaagt ttggttctgg tgaaggtccc atatttgtta
     5401 atgtatgaag cagacggaaa tcattctttg taactaatga acgaccatct aatcccatac
     5461 ctgcaatgga taaagtagcc cagataggga atcctgagaa taattggtta tattcaggtg
     5521 tacgcgcgaa tttgaccata cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt
     5581 ctttttctgt gattactcca ttatccaaat cacgttggat atagatatca aggaatgctg
     5641 agatacgtcc gattgacatc gcagcaccat tttgtgattt gattgctgct aaatatccga
     5701 agtatagcca ttggacagct tcttttgcat ttgcagctgg tttagaaata tcatagccat
     5761 atttcgcagc catttctttc atttgcgcta atgcacgata ttgttcagtg atttcttcac
     5821 gtaaacgaat cacttcttca ctcattgtat gataaccagt catatcatga tcttttttct
     5881 tttgttccat caaatagtcg attccataca atgccacacg acgatagtca ccgatgatac
     5941 gtccgcgacc gtaagcgtct ggaagccctg taatgatttt gtttttacga gcagcacgca
     6001 tttcagcagt ataagcgtca aatacacctt gattatgtgt tttgcgccag tcagtgaaaa
     6061 tatgtgtcaa ttcatcgttt ggtgtatagc cattgctttt tagcgcattg ttagccatat
     6121 tgattccgcc gaacggcatg aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga
     6181 ttttttccaa atctttatcc aaatagcctg catcgtgtga agtaatagtt gatacgattt
     6241 ctgtgtccat atcatagaca ccgttacgct cgaattggat atcatttaac ttttgcaatt
     6301 catcccataa tttttctgtt gcttctgttg ggccttccaa aaagctgtca tcaccgcgat
     6361 attcagtgta gttattttga ataaagtctc tagtgtctac gccttctttc catttggtac
     6421 ctttaaatcc attccattgc tccattgtat ggacctccta aatttctgtt ttatgtaaca
     6481 gtttttacgt gattagtata acacagcacc ctatctttgc aagggttttc ttgtttttta
     6541 tttaacaatt tttatgtttt atttacatat atttaaatca acgttgacat accaacattt
     6601 aagcattgtg aacactttca tctttttttg caaacgtata aggatttatt aaaaatgaat
     6661 gaaaaagaga cgccacctgt attaatacag atggcgtctc ttcattaaaa tactacaaca
     6721 gttacgactc gatgaaaatc tcaggagcat cgataacttc gatgacttct ccaccttttt
     6781 tctcatccat cgcaaatgat ccattcgatg ttcgatcacc tatagggaaa tcagttgggt
     6841 caatcgttaa ctgctgccct ttttgattca tcaaggttat ttcttttgat cttccttccg
     6901 acatatagat aatacggtgc ggattttttt tcagctcacg catgaccgtc actccccgct
     6961 tcgcacgtga agtttggctg atttcttgag caagcatgcg tttaacagct cctcgctgag
     7021 taacgattac gatcggcgta tcaccttctg ctaacacaag cagcccgttg ataatcctat
     7081 ctccatcttt caaattcata gacttcactc cagctgcctt tgctccaaca acgggaacat
     7141 ctgttaaagg atagcgcaaa cccattcctt gatgcgttac taagaaaaca tctaagcctg
     7201 gctgatcttc tttcagatac acagcaatca gttcatcttc ttccgccttt agcttcatac
     7261 agctgagcgg acggctctta taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct
     7321 tgatcaatcc attcttgctg atgaatacaa atgttttctc tgcttgaatt ttttgatatg
     7381 ggaaaacagc taaaatcgat tcgtctacag acagattgga gattgtttga gaaatgtgct
     7441 caccagcatc tttccatttc aaatcgggta gttcatggac agggcggtag atcacatttg
     7501 ctttatttgt gatcagaaga acatggtcca atgtactcaa ttcacctgag tatagcaagt
     7561 agtcgccttc tcgcatcccg atttcttctg gtttggaagc actataagaa cgaaggcttg
     7621 ttcgcttcac atagccttct cgcgtcacac tgacaattac gtcttcttgt gccactaaga
     7681 cttgcgtatc aattttgatt tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag
     7741 aataattttt tttcacttct cgcaattctt ttttcataac agagaaaagc tctttgtcat
     7801 ttgaaaggat tttattcaac tctgtgattt gcgcaatcaa gctttcggat tccttttgaa
     7861 gctctgtgat atccgtgttc gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt
     7921 gttcttctgt aaattgaaag acgtcgacta aattcttttt ggcgtctttt ttgtctttac
     7981 tttcacgaat cgttgcgatt acttcatcca gaatcgataa agctttcatc aatccttcta
     8041 cgatatgctg tcttttttga gctttggcta aatcgaattg actacgtttt gttatgactt
     8101 gctttcgatg ctcgatataa cttcttaaaa tgtctttcag tccaacttga tgcggagtca
     8161 tatggtcgat agcgaccata ttgaaattgt aattgatctg tagctcagtg tttttgaaca
     8221 aataattcaa aattccttga gcattggcgt cttttttcaa ttcgacgaca atttgcagac
     8281 ctgttctatc actttcatct cgtacttcag ctattccatc gattttctta ttcaaacgga
     8341 tctcatccat ttttttcacc aaggtcgctt tatttacttc atatgggatt tctgtgatca
     8401 cgatttgctg tttattaccc ttcattgcct ctatctttgt tttcgaacga agaataactt
     8461 ttcctcgtcc tgtttcataa gcttttttga tttcatcttt tccttgcaag atccctcctg
     8521 ttggaaagtc aggtccagga atatattcca tcaatttttc aaggctcgct tgtggatgat
     8581 cgatcatata gattgtgcca tcgatcactt cagctaagtt atgagtcggg atttctgttg
     8641 catatccagc agatatacct gtcgatccat tgacgagaag gtttgggtat cgagctggaa
     8701 ggactgttgg ttctttttct gtatcatcaa aattccacac gagatcgact gtttctttct
     8761 cgatatctgc tagcatctct ccacttaact tggataatct ggcttcagta taacgcatcg
     8821 cagcaggtgg atcaccgtcc atactcccgt tattcccgtg catttcgatc agtacttccc
     8881 gcaacttcca gtcttggctc atgcggacca tcgcttcata aatactgatg tcaccatgag
     8941 gatgataatt ccccatgata tttccgacag attttgctga tttacggaat cctttgtcat
     9001 acgtattgcc atctttgttc atggcaaata atatccggcg ttgcactggc ttcaatccat
     9061 cacggatatc aggcagtgca cgttcttgga taatatattt ggaataacgt ccgaagcggt
     9121 cacccatcac ttcttctaaa gttaattctt gtacttcttg tcgattttcc atcaggtgac
     9181 ctcctattct acttcaaata actgattgtt ttcattttta tctgctcgtt cttcatctaa
     9241 taaatcatta gaaactgaag gagagatttc agtatcctct tttttatcta agatactgcc
     9301 atcttcttcg agcgtaaatt gcacgtgatt ttcgatccat tttcttcgag gttctacttt
     9361 gtcacccatc agtgtggtta ctcgacgttc agcttgcgca gcatcatcga tacggacgcg
     9421 gatcaacgta cgggaagtcg gatccatcgt tgtttcccac aattgttccg cattcatctc
     9481 tccaagtcct ttatatcgct gcagcatata tcctttgccg acttttttaa tcattgctgc
     9541 caattcgtca tctgtccacg catattcaat gacttgcttc tttccttgtc ctttggatac
     9601 tttatataaa ggcggtaacg cgatatatac tttcccagct tcaatcagcg gtttcatata
     9661 acgataaaag aaagtaagca gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt
     9721 catgatgatt actttgtcgt aattacagtc ttcgatagaa aactcaggac cgactcctgc
     9781 accaatcgta tagatcatcg tattgatttc ttcatttttc aagatatctt gcattttagc
     9841 tttttcggta ttgatgactt ttcctcgtaa aggtagaatt gcttggaact ttctgtctct
     9901 tccttgttta gcagatccgc cagcagaatc cccttcgact aaatacaatt catttttctt
     9961 agggtttcgg gattgtgcgg gtgtcaattt tcctgaaaga agagattcgc cttttttacg
    10021 cttcttccca tttcggcttt cttctctggc tttacgtgct gcttctctcg cttctctggc
    10081 tttgattgct ttacggacca acatctggct catttcgcta ttttcctgca aataaaagcc
    10141 catttgttca ctgatgacgt tatctactac ggtacgagca actggcgtac caagtttttc
    10201 ttttgtttgc ccttcaaatt gcagaagatt ttcaggtaca cggatagaaa gtacagcagc
    10261 taacccttct ctgaaatcac taccttccag attcttgtcc cgttctttca gcaaaccaac
    10321 ttttctcgca tattcattat aagatttcgt catcgccgct ttcataccag cttcatgggt
    10381 tcccccatct tttgtccgaa cgttattgac aaaagataaa acattttctg aataaccgtc
    10441 attgtattga taagcgacct ctacttcgat accttctttt tctcctgaaa aataaacaac
    10501 tggtgtcaat gtatcttttt cttcattcag ataatcaaca aattctttga ttccttcttc
    10561 gtaatgaaaa atttccttga ctggttcttc tccacgaaga tcagaaagag agattttaac
    10621 gccttttaga agaaaggcag attctcttaa ccgttcggct aaaatttcat atgaaaattt
    10681 cgttgtactg aatattgtat catctggtaa aaaatggaca gtcgtcccgt ttgctttttt
    10741 tgtttttcct atctttttca aagtaccgtc aggtttaccg ccgtttttaa atttctgctg
    10801 atactctacg ccatctctaa cgatcgttac aattagccat ttcgataagg catttaccac
    10861 actggctcct acaccgtgca gtccaccaga agttttgtac cctccttgac caaactttcc
    10921 gccagcatgc agcacagtaa aaatcacttc aactgttggt attccggaag catgcattcc
    10981 tacaggcatc ccacgtccgc tatctgcaac agttatacta ttgtcttcat ggatggtcac
    11041 atcaatctca ctgccataac ctgataatgc ttcgtcaact gcattatcga cgatttcata
    11101 gactaaatga tgcaagcctc ggctgtcagt ggatccaatg tacatccctg gtctttttct
    11161 aactgcctct aatccttcca atacttggat ggacgagtca ttgtattcgt tgtttacttt
    11221 ttttgccaag gcaaacgctc cttcaataat actttgcggc aaaaccgcac cacgattatc
    11281 atactagaaa aggtcgtgaa aaaaaagagg gtagacgaga aatcaatttt aattatctcc
    11341 taaccgcttt ttcgacattc ccctctatcg atcatctctt ctttgtggtc atttttattt
    11401 ttggttttct ttcaacttac caagttcaat cttaatacaa cgatccatta cgatatctgt
    11461 acgtcctgct tttcttagca tctcagctgc ttctttgttt tcaatcccta attgtgccca
    11521 gaacacttta gcatcagttt tcaaaaaatc acctgctatt tcaggtaaaa attcactccg
    11581 tctaaataca tctacgatat cgattggatc atcgatttct gtcaaattcg catagacttt
    11641 ctcgcctaga atttcttcac cagccaataa tggatttact ggtatgattt tatatccatt
    11701 gtcttgcaat acctgtgcaa ccatgtaact tgttcgttct ggtttattgc tcaagcctac
    11761 gacggcaata ttttttgctt cttccaaata gttgaaaatc gtttcttgtg ttggattttt
    11821 tacagacatt tctgccaccc cttgcttttc tttttattcc atctattata cttgtatctt
    11881 tcattttttc aattgttcct gttacctatt catgctaaaa taaaggatag ttttcaactt
    11941 gaaagagggt aaaaaatgaa attgatctta ttacttattt tagcatatct tttaggttct
    12001 attccttccg gtgtctggat tggcaaattg tttttcaaaa aagatatccg acaacacggg
    12061 agcggtaata ctggaacgac caatacattt cgtgtattag gcaaaacagc cgggattgtt
    12121 gtcctgctta tggatatttt aaaaggtaca ttggctactt gtttgccgat gatcttccac
    12181 ctttctggga ttaaccctct ttggtttggg gtatgcgcca ttcttggcca tacatttccg
    12241 atttttgctg ggttcaaagg aggaaaagct gttgcgacaa gtgcaggaat gcttttagga
    12301 tttaatcctg tattttttgt ttattcttgt atcatcttta tcgttagttt attttgtaca
    12361 agtatggtga gtttgagcag tatgatcagt gcagtattga tcactttatc tacgatcgtc
    12421 cttccttatg tagctcctgt gattttggct aagcccaatt ggttactgac gatcattgcc
    12481 tttttagttt cttcctttat cttttatcgg cataaagaca atatcaagcg tatcagaaac
    12541 catactgaaa gtcgaatccc gtttggtttg aatagcagca agaaaaaatc ctgatacttt
    12601 ttcattccaa agagataaaa cgggagtgtg acaaatcgat gtcacactcc cttattactt
    12661 acaggatagc ttttttacac accttggtaa acggtgttcc ctgctaactt ctcggtcaca
    12721 acctcttact tcactgtgat ggaatatttt gtcttgaaga tttcatgggc acctagttga
    12781 ttgatcccta ctttttccat caagtttcca ctactgttca ccgtgtcagc gattccgcac
    12841 cacggttcga tacaaacgaa aggcgcttct tttggataag gagaccagat ccctacataa
    12901 ggcatatctt tatagcttaa agttacgcta tgggaagatt tttcacttcg aatggcgtac
    12961 gcatttagac cccttgtttc aaaaaccaag gcatcttgct caaaaagttc acgattcaaa
    13021 gccagattcg tatttgtctg acctaacgtc ttttgatcca tatcgatgaa tgggcctttc
    13081 aatggtaggc gtaatcttga tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac
    13141 aatccttctt caagaggtac attgaaagca ggatgtccac cgatagaaaa gaacatttct
    13201 ttttcaccgg tatttgccac ttgataatga accgtgatgc cgtctcctcc taattcatag
    13261 ctgatcacta attcaaaatc aaatggatac ttttctaatg tctccggcgt actccttaag
    13321 atgaaactag ccatttcttg tccatgttca atgacttcaa aatccatatc ccgagcaaat
    13381 ccgtgttgcc ccatttgata tgttttccct tgataaacat actgatcatc ttttaaacgt
    13441 ccaacaaaag ggaataaaac cggtgcatgt ctgccccaat acgctggatc accttgccag
    13501 atatactcta aaccatgttc ttttgatttc agactgatca attccgcacc gtgttcagcg
    13561 atttcggcga tcagctggtc attttgaata ataacagtca ttgccattcc tccaacttcg
    13621 acttactttt taaaatggga aggctggaac aaacatttct atctgcccca gtccccttac
    13681 ttctaataac tgtgttttgc taactcgtct ttaaaacggg aattcaatac ttttaaataa
    13741 gttcccttca tgcctaacga acgagattca atgattccag ctgattccaa ttttcgtaaa
    13801 gcattgacga tcacagaacg agtgatgccg atactatcgg caatattaga agcagtcaga
    13861 cgaccttcat ctccatccaa tgcttcaaag atagcttgta cagccttcaa ttcgctataa
    13921 gataatgtgt tgattgccat ttggacagcc gttgcactcc gtacatctgc ttcgatgctt
    13981 cgagacttct gataaaggat ctgcataccg acaaccgtag ctccatattc tgctaagact
    14041 agatcgtcat ctgcaaacga ttgttccata cgagataaga taatcgtacc caaacgttcg
    14101 ccagctccaa agatcggaac gacagtcgtc agaccaaatg gatatttctc tcttgactct
    14161 accggaaatg cagtaatatc actagtaatt gggatattag cttcagtttt catcaactgt
    14221 gccactgtat cagtataaga ttcaggaaat tgtttctcaa caaacattgt tttgattcgt
    14281 tcattgttca catcgtgacg ttcattataa cctagtaata ctccgtcact tcctatgata
    14341 taggtgttgc tgtcaagaat accacctagt gtgacagcca ttttatcgta aggcaaatct
    14401 gcttgcatgt caaaagtatt cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt
    14461 agcaagtctg tcattgtcgt cctccgattt tttataaaat atagcgactt aaatcttcat
    14521 ttgccgcaat tgagccaagt ttgtcatcca catatgcttc tgtgatcgtt atttctccca
    14581 tttgcatatc tggtgcttca aataatagat cttctagtag tttttccaaa atggtatgca
    14641 gtctgcgtgc gccgatatta tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc
    14701 gttcaattgc ttcttttgta aacgtaactg acacattttc tgtaccgatc aacgcgacgt
    14761 attgttttac taaagcatta tttggttcgg tcagaatctt cacaaaatct tcagctgtca
    14821 aatcatctaa ttctacccgt atcgggaagc gtccttgaag ttctggaatt agatcacttg
    14881 gtttagacaa atggaaagct cctgaagcaa tgaataggac gtgatctgtt tggatgatcc
    14941 cgtattttgt gtttacttgt gaaccttcga caattggcaa tatgtctctt tggacgcctt
    15001 ctctggatac ttctccagat tgctgtgatt tagaagtgat cttatcgatt tcatcgataa
    15061 aaataattcc actttcttcc gctaatttga ttgcctcact atggatatca gcatccttga
    15121 ctaattttgc tgattcttcc ttgatcaata actctaaagc ttctttgact gttaccgtcc
    15181 gttccacttt tttcttcggt gtcaaagcac ctagagtttc atttaggtcg atccccattt
    15241 gctccaatcc attgttcatc attggtgcag gtttcttttg gtcttcaact tcaatggtta
    15301 cttcacgact atccagtaaa cccttttgta gttgttccaa gactgtacgg cgattgattc
    15361 gaatatcttc tgtcacttct tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa
    15421 gattcatcat ttgttcaaat tgattagtgg attgtttctg ttcttttttg ataccaggta
    15481 ccaagatttt taccaaacgt ttttctgcat tttttttagc acttgtgtat acacggctgt
    15541 actgctcttt ttctacgatt tggatcgcat tttctactaa atcccgaacc attgattcta
    15601 catctcttcc gacataaccg acttcagtaa attttgttgc ttcgactttg acaaatggtg
    15661 cattgacgat ttttgccaaa cgtcgtgcga tttctgtttt accgacccct gtaggtccga
    15721 tcatcagcat attttttggt gtaatatctt gttgcatttt ttcatctaac tgcaaacggc
    15781 gatagcggtt acgcaaagca acggcaacag atttttttgc tgcgtgctgt cctacgatat
    15841 attcatctaa ttcacttacg atttgtcttg gtgtttgttt taattcatac atatctactc
    15901 acctcttaca gttcttctac gataatatta tgattagtaa agatacagat atctgcggca
    15961 atatttaacg cattttctgc aatttctttc gcactcatgt cgttttgagc atagtttttc
    16021 attgctcggg ctgcagccaa cgcaaaattc cctcctgatc cgatagctaa gataccatca
    16081 tctggtgtga taacttctcc tgtacctgaa acaagaagca tttctttatc attcattacg
    16141 atcagcatcg cttcaagttt ttgcatagat tgctgtgtcc gccattcttg agctaattcg
    16201 acagctgcgc gttgtagatt cccgttatat tcgtttagtt tgccttcaaa tttttcttct
    16261 aaagtaaacg catctgcaac acttccggcg aaacctacaa caacttctcc attgtagata
    16321 cgtcgtactt tctttgcagt gcctttcatg acaacagatt cacccattgt tacttggcca
    16381 tctccagcca tagcaaattt cccgtctttt tcgacggcac agatagtggt tgaatgaaat
    16441 ggtgattcga ccatgtttgt ttcctcctaa atttgttctt ttccttattt ttacgctctt
    16501 ggatggaaag aacgatagtt tttttgtaaa ctttcttttg ttacatgggc atagatctgg
    16561 gtagtcgata aattggcatg tcctagcaat tcttggactg tccgcaaatc tgccccatta
    16621 tttaataaat gagtagcaaa tgtatggcgc agcatgtgag gatggatatc actatccaaa
    16681 ctgctttttt tgatcatttg attcaagaca tattcaattc cagtaggcgt gattggtttt
    16741 ccatgatgat tcacaaaaac gatatcatgc tcttcgtgat attttgtcat caatacctgg
    16801 cggccttccc tgaaatattc ttgcagagca tcttgagcaa aagaacctag aggagcatat
    16861 ctctctttgt tccctttacc atggatcaac atgacctctg aatcaaaatc aatgtctgat
    16921 accgacaaac tgctacactc acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa
    16981 gcgcggttcc tcaaatccaa aggactatct cctgctacgc tgtcaaaaag tgcttgcatt
    17041 tcattttcat aaaaaaaacg tggcagtctc aaatttttct ttttgagatg aacgtaagaa
    17101 aatggatttt cttttatcac ttcttctttg agaagatact gataaaacga acgtaggctg
    17161 gctatcttgc gactgataga atttctgcta tagctaggac gatgatcagc taatgcactc
    17221 aaatagacac gaaccgtaaa atattccgcc ttcagcagat cagcgtctcc agattcctcc
    17281 aaaaaatgtt tgaaatggag aatgtcttct tcatatgctt ctttcgtttt ttctgaatat
    17341 cctctttcga caatcaaata acgcaaaaat ttttgttccc acgtggcttc tgtcattttc
    17401 tcaccttcct tttgtcccag agaataattt agcacagcgt ttataaaaag acaaataaat
    17461 tgtcagaatt taaccaatat ttctaagttt gttcacaatt ggcgcaattc ctcacatttt
    17521 gtttgagaaa ttcttaagaa aaacgcaaga aaaagagcga gactatccgt ctcaccctct
    17581 tcatggagtt tccttactca tttgggattg cagttttttc taattctgtt tttattgcct
    17641 cattagcttt taaagctcgt tgtgcaattg cttcatatct tgcttttttg tctcgaatgc
    17701 gctctggcag ttcaggaaac agaccatagt tggcattcat cggttggaaa tgttttcctt
    17761 ccgcatgagt aatgtagtaa gccatacttc caatagctgt ttcttgtgga aactcgactg
    17821 gttccatttc ttttgctaag cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac
    17881 tttcaacata gccttctaca ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg
    17941 ttgattggta agtcggtttc agcaattctg gcgaattcat aaagctgttt cggtgcatca
    18001 caccatagcg aacgaattca gcattttcta acccaggaat catccggaaa acacgttttt
    18061 gctcgcccca tttcaagtga gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat
    18121 tgtcttgacg cagctggata acagcataag gacgtttgcc agttttagga tcttccaatc
    18181 caactggttt cataggtccg aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa
    18241 ttggcataca tccttcaaaa tatttttctt tctcaaaaga tttaagttct gccacttcgg
    18301 cattgattag tgcctcatgg aaagctttga attcctcttc tgtcatcgga cagttcaagt
    18361 aagctgcttc tcctttgtca tagcgggact tcaaatatac tttatccata tcgatcgtag
    18421 ctttgtctac gattggtgca gctgcatcat agaaataaaa accatctgaa ccattgaatg
    18481 ctttgatgct ttctgctaat ggatgagaag tcaaaggtcc tgttgctact acggtgattc
    18541 cttccggtat ttcagtaatt tcttcgttta tcactgttac taatggatgc tctttgactt
    18601 ttcgggtaat tgtttcagaa aaagaatcgc ggtcaacagc taaggcacca ccagctggca
    18661 ctgctgtttc atctgccgaa gtgattacta ccgaattcaa tcggcgcatt tcttctttca
    18721 ataaccctac tgcattagcc aaactgtttc ctcttaatga attggaacat actagttcag
    18781 caaaattact tgtgtgatgc gcctcagtcg atttgactgg acgcatttca tataaacgga
    18841 cagggactcc tgcttgtgcg atctgccaag cagcttcact tccggcaagc cctgctccaa
    18901 caacatttac cattttcaat gaaagaacct cttttctatt atttttggat gtcttcttcg
    18961 taatctccat ttacacagac tacttgttta ccgcctttga ctttcttctc gaccagatat
    19021 ccgtcacatt ttggacaatt tctgcccact ggtttatccc aagaagtgaa ttcacattct
    19081 gggaaacggg aacaaccata gaatagtctg tttttcttag attttcgttc aatgacttgt
    19141 ccttcatgac atactgggca agtcacaccg atttctttta cgattggttt cgtattccga
    19201 caatcaggaa aattactgca agcataaaac ttcccatatt tccctaattt tatcaccatc
    19261 ggatgtccac agacatcaca atcgaatcct gctggttcat ctttgatttg aattttttcg
    19321 attttttctt ccgcattcgt cagttctttt tcaaacggtt tatagaaacg atcaaccact
    19381 ttgacccacg cttccgtgcc ttcttcaatt ttatccaaat ctccttccat ttctgctgtg
    19441 aaatggatat cgacgatttg agggaagaat tcacagatta atgaattgac aatttctcct
    19501 agttcagtcg gctcaaatct tttttgtgtc aattttacat agtagcgtct ttggatcgta
    19561 tcaagtgttg gtgcataagt ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc
    19621 aacgtagcct cactaaaacg tgctggtggt tgagtaaaat gttgttttgg ttcaatatca
    19681 acagattgga ctacatcgcc ttcttcaagt tctggaagaa tattttcttt ctcttctttg
    19741 ccgtcatctc taccttccac atacacttgc atgaatcctt tgaatttgat ttttgaaccg
    19801 ttcgcaatga agatgacacc attttgttct aatgtcactt tcattgtatc taaaatagct
    19861 ggtgtcatct gactagccac aaaacgtgac cagatcaatg tataaagttt caattgatct
    19921 ttatctaaat attgtttgag ttcatctggt gtacgaagca cactcgttgg acgcacagct
    19981 tcatgggcat cttgtgcgcc ttgagcattt ttgatctttc ggtgatcatg tctagaaaat
    20041 tcttttccat acgtatcttc aataaatgct gcggcctctg cttttgctgt atcggcaatc
    20101 cgggtagaat cagtacgcat gtatgtaatc aagccgactg ttccttgttt acccaaagtg
    20161 attccttcat ataattgctg tgctaccatc atggtttttc ttgttcgaaa gtttaactta
    20221 cgggcagctt cttgttgcag gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc
    20281 tctttctttt ctactttttt aacttcgaag tctgttccat caattctagc agtgacttct
    20341 tttacacttt gtgcatctgg taatttcttt ttcttaccgt cgattcccca gaaatttgct
    20401 ttgaatttct tgcgagcttt tttaaaatta ccgtcgatgc tccaatattc ttctggaaca
    20461 aattctcgga tctctttttc acggtcaata ataattttta aagcgacaga ttggacacgt
    20521 cctgcactta accctttctt gaccttacgc cagagaatag gactgatcga ataaccaacg
    20581 attctgtcta aggtacgacg agcttgctgt gcatctacta aatctacatc gatcgttctt
    20641 ggttccttaa aagctgcttt gactgcgtct tttgtgattt cattgaagac cacccgattt
    20701 ttttctttca gatccaaccc aagtaggtac gctaaatgcc aagcaatcgc ttctccttct
    20761 ctatccgggt cacttgccaa gtaaactttt tgagcttttt ttgctgcggc tttcaggctt
    20821 ttgatgacat cgccttttcc gcgtatagaa atataatggg gttcataatt gttttcaaaa
    20881 tcgataccca ttttactttt cggtaaatct cttatatggc caacactggc cattacttta
    20941 tagtttcgtc caagatattt ctcaattgtc ttggcttttg caggtgattc tacaatcact
    21001 aaatatttat acgccattac tgaatggact cctttccatt gtcgtgttcc tactattaaa
    21061 agaggattca tacaaatcgt agctaatcat atccattcag aaaacgtttg tcaagaaaag
    21121 caaatgaaaa gtatggtgtt attctttaaa ttctgccaag atatcttcac ccgaaaccgt
    21181 gcaaactgca ccgtcttgga tcaaatgatg acatccggta gaatgttctg ttaaaatatt
    21241 tcctggaaca gaaaaaactg ttcttccaag atccaatgct tgctgtgctg ttatcagaga
    21301 gccgcttttt ttcttggctt caaagacaca tgttccttta gacaaagctg cgataatccg
    21361 atttctcatt ggaaaacaat gctttttcgg gccggattga ggaggatatt cactcaaaag
    21421 aagatgatca tttgcaatca cctcttgcaa gtcacgattt tcagcaggat agacttgctc
    21481 tatccctgtt ccaatgactg cgattgtccg tccaccatga gagattgctg cttggtgacc
    21541 gtatgtatct atccctttcg cgcagccact gacgatcaca tatccagctt ctatcactgg
    21601 ttttagtaaa taataaactg tttttttggc taaagcagaa gtgtgtcttc caccaacaac
    21661 gcttaacatg ttacaagaaa gtaaagatag atcaccatta taaaacagta caattggcgg
    21721 ataggtcaag tgaagcagct gagggggata tttgggatca aagcaagtga taaacgactg
    21781 tttcgattgg attttgcccc agttagcgtg aatttcctgc caacttttgc gaaacagctc
    21841 ccggtgtttg gttattttac ctatcttaat gatgtcttca aatccgattt ctttataagg
    21901 atactgcatc atccagttag caagtgccca ttttccttga ctactgattc cttttgtata
    21961 agcaagtttc aataaatttt cttctatttg atacatcata aaaccgcctt tcgtctctct
    22021 ttctaataag atacgtaaaa gcggctgttt tttattgtaa atattttttt acaggtgcaa
    22081 aacttttacg atggattggc gtaattcctt gtcgttcaag tccttctaag tgttcctttg
    22141 ttccataacc tgcattatgc gagaaaccat atccaggata aagcacgtca tattctttca
    22201 tcaattggtc tcgatatact ttagcaatga tacttgcagc agcaatcgac acagatcgtg
    22261 catccccttt gattattttt tcctgctgaa taggcaaatc caaggtcatc gcatcgatca
    22321 acaaatagct tggtacaatg gacaattttt ctactgcttg gatcatcgct aatttacttg
    22381 cttcataaat attcacatca tcgattgtct gctgatcaac gatcccgatc ccgattgcca
    22441 gggctttttc ttgaacttgt tgataaagaa gttctcgctt tttctctgat aattgtttgg
    22501 agtcattcag ccctaatatg atctcatttt tcggcaagat aactgcagct gccacgacag
    22561 gtccagctaa tggtcctctt cctacttcat caatgccggc aatccattgg tgaccttgtg
    22621 cgtacccttg cttttcaaag caaaacattt cttcatattt ttccttaatt aatgcttcct
    22681 ttttcaatcg tcgttcaaaa gaagccagtg cttgctgcac gcctttgcgt ggatcatttc
    22741 ttaatgattc tagtgtagga tcatctattc tctgtatgct tttcaatcgt tctttgatac
    22801 tttggatgga ttcagtcatc acttttttgc tccatttctt cgcaacgatc cagtgtgtat
    22861 cgtccaagtt ttccttgacg aatatcatgc acgactaatt cgctgccacg atcatagtct
    22921 tctttatacc cacgttttgc tgtaatatcc atcagtaatt ccggtgccaa tttttcttct
    22981 tgctcgattg ataaaccata acgatcgctg attcttcctg aataatatcg ggcaaaaaag
    23041 tctaatccgt aaatcgctaa atcatctaaa tggacaagct ggtctttgat cgcgcctgtc
    23101 agcgctagtt ttttgccgat ttcttcatct tcaaatttgg gccataaaat ccctggtgtg
    23161 tctaacaatt ccaactcatt acctaaacga agccactgct gaccttttgt tacgccaggc
    23221 ttatttcctg tctgtgcaat tttcttgcct actagtcgat tcatcaacgt agatttccca
    23281 acatttggaa tacctaagac cattgcccga atagcgcgag gcttgatccc ccgagcagca
    23341 tctcttgccc gtttttcttt caatgcttct ttagccgcag gtactagacc tttaattcct
    23401 ttactttctt gtgccgtgat agctaatgca agatggcctt gtttacgaaa atattcttgc
    23461 cacagctttg tctgttgtgg atctgccaga tccgctttat tcaataaaac aaggcgaggt
    23521 ttttgctgaa tgatctgatc tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct
    23581 accaattcaa atacgatgtc cacatatttc attttttcag aaacttctcg tcgggctttt
    23641 gccatatgtc ccggaaacca ttggatggtc attgttctct ccattccttt cataccggaa
    23701 tattccccgg ctttacttgt ttttttcttt ttcaatataa attcactttt ttattataaa
    23761 gaaattccat ctattagtta agtgaaagtg aaccagtcct cattctatca catcttcact
    23821 tttttgatga tgcaaatttt tattttggtt tctatatatg aatctaaatt tttgacagcg
    23881 tttcctaaat aagagatgat agacgaacaa aacagaaagg agacgaaaca aatgaatagt
    23941 atggtttttg ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag
    24001 ctgggtttta aaaaaaatga agaattttct actgaagaag ctggttcaat ggtatgggat
    24061 gacacttttt ggataatgct tctctctcat gatttttata aaaaatttat aaaagataaa
    24121 gaaatagctg ataataaaaa aacaagtggt gctttgattt cgtttagtgt tgaaaatgcg
    24181 gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt ccacgaagta
    24241 gatatgggaa tgcctgaaga agaaatgttt tcattagaag tccaagatct agacggaaat
    24301 acattagaac ccgtatggat gaagatgtaa gcagataact aatctgacgt tttttataac
    24361 aaaaaaacaa aggcggatga tacgataagt attctccgcc tctatttttt ttattcgtac
    24421 aatctctttt ttacagttta taaaagcagt cttctcaccc tttttttaat tgttagatgc
    24481 cagtgtatgt aaatcaccaa tgtgtcatga cacattggtg attttatggt atccttttat
    24541 tattaactga caaaggagag aatattttga acactttgat tgcaaaagac gtttttagat
    24601 tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct tttattagcc cttgatgtca
    24661 tcttatacaa gatagcgatt ggtcctgctt attttcaaat cacattcgga tttttaagta
    24721 tggcgcttat gggttatcta tatggaccga tctgggctgg tttgcttgaa atgttgtcga
    24781 acactttaaa tttcacgata tttggttcag gaacgtttca agctgctttt cttatcacgg
    24841 ctttccttgg tggatttatt aatggcttat ttttatatca taaaaagata aacctgcctt
    24901 ccgtcatcgc tgcccaatta ttgattatga tcccagtcag cctttttatc aattcttggc
    24961 tgatgtctgt tttgtttggt gcagatttca aagtaatttt tctaggtaga ttcatcagga
    25021 atatgatcca aatccctatt caaataatgg ttgtttattc atttatcaaa actttagagg
    25081 ctagaaaagt gtttaaacga gctgtttgat tttacgtact aaggtagtaa actgattttg
    25141 gattaaacaa taaagcgtgc agtctcaaca gactgcacgc tttattgttt ctcactggat
    25201 ccattttatt tcatcaagtg gataatagac aaatcttgct ttacctagaa tatcagtttt
    25261 attgatcgtg ccaaacgaac gactatcttt tgaaactctt cgattatctc caagtacaaa
    25321 ataattatct tccggaagct tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt
    25381 ataggatgca tttgcatgat ctgatttcag gtttttagtt agataaggtt ccttaactac
    25441 tttcccattg atcttcaatt gatcattttg gtagctgaca ttttctcctg gaagaccgat
    25501 tactcgtttg atataaatcg tgccatctgc taattgaaaa acaacaatat caaatcgtct
    25561 gatctccgaa aatttttcca tcacaaccat atcaccttgc ttcaacacgt tttccattga
    25621 attgccttcc actggtaccg ggatgaataa aaatccccgc aacatgaacg caaggaatac
    25681 agaaacaagt aagtatttga atacaagcca gaagcgatta atatagcgtt gtttttttgt
    25741 caatctctat caatccatcc ctcttttttt cattctttct ttattataag aaaaatatca
    25801 aaaaaatgcg actagatagc tgaaataaaa ataattcttt caattaggct attggacaaa
    25861 aaactttttt gatcataaag tacaaaacta tctcttatat gtcatgatta ttttctaaat
    25921 atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca ttcagttcca
    25981 gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg tctttaccgg
    26041 cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat
    26101 aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa aggcagcccg
    26161 gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt gatggtcttc
    26221 ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct gttcgaaacg
    26281 tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt cttctgtcaa
    26341 ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga aagcgtcagt
    26401 tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca atgttggatc
    26461 atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt gttcataagt
    26521 cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac caggaagcgt
    26581 gtcgaaaggc tggatcatac accagaagca gcctcctgca aatattgctc gttccattat
    26641 gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc ttgctttcag
    26701 tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag gtatcttttg
    26761 gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct gctttaatat
    26821 acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga tcatctgtat
    26881 tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc acatctttga
    26941 ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca tttccgtttt
    27001 ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt acttcgaaag
    27061 taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca ctatcttttt
    27121 gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt gaattgatcg
    27181 ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt tctggttccc
    27241 gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc ccaagaagga
    27301 taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat acgtcagatc
    27361 ttgttttcat gtttattgat catcctcctt ctctaaccat tggttttgtg tctcgatcag
    27421 ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat ggaaatgatc
    27481 ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc tagttgtttg
    27541 atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa gcaaatcatt
    27601 gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc ctttgttcca
    27661 attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa atggattgta
    27721 gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt ccaatagttc
    27781 tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa
    27841 taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg tgataaaatc
    27901 agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta agatctggtc
    27961 acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat tataacggtc
    28021 ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat ctcctactcc
    28081 ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat ttggatcttc
    28141 tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag cagctaaacc
    28201 tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcaaat gtcgtttcgt
    28261 taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa aattttatca
    28321 aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata aagaaaaaga
    28381 gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa tctacataat
    28441 acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc gtatgcagta
    28501 cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct tgcgccaatt
    28561 cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct ttcagttcat
    28621 ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta gttacaggga
    28681 ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta gacagcaaac
    28741 cagttgttcg gctgattctt ccacctttta ccaagttatc tagtgtggat attccgatgt
    28801 ataattttgt attgtcttgt acttttttga cttcttcaag gattttctct actgttttac
    28861 cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg tcagtggtat
    28921 cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt gctgcttcta
    28981 ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg tcttctccta
    29041 gctcatcata catcgagatg aattctccaa ttggtggttg gcttgttttg ggcagatttt
    29101 ttgctttcgc catcatttcc ataaatcttt cgccgtctag ttggtcatca tcgggatata
    29161 ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca tcgcgtacat
    29221 ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt attttctctc
    29281 tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca atttctattt
    29341 ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg agccgttttc
    29401 atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc
    29461 acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt
    29521 gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt
    29581 cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg
    29641 ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt
    29701 tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt
    29761 gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa
    29821 gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt
    29881 tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg
    29941 ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg
    30001 tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa
    30061 aaataaagct atggatggga cagctctaaa taacagctag tctatccata gcttttttat
    30121 atcgtcaatt cgcctgtccc gctttttaca ggattctcat tacgctgata cgtttcaaat
    30181 tgatgcggat aacgattttt ttcatccatt tctccttgac tagtattgat cagtgaccaa
    30241 tcagaccagt caacaggtgg aaaataagca tctccatcga atacatgatg gatgactgta
    30301 cggtataata tcgtacaaaa tggcagaaat tcttgataaa cagctgaccc tccagcgatg
    30361 aaaaagatac cgtctgtttc tttttccaga gcaagcacgt cttccacact atgcatgaca
    30421 tgcacgcctt ctgctttata ggaagaatct ctcgtcaaaa caatcgtctg acgattcggc
    30481 aacggacggc tacccatccc ttcgaatgtt tttcgtccca tcactagtgt atttgcttct
    30541 gtcatttgtt tgaagaattt cagatcattt ggcaaacgcc aaggaagacg gtcttctttt
    30601 ccaatcaatc cgttttcatc ctgtgcccaa attgcagcta acattttctc atcctttctg
    30661 attctaaaca gcgataggag ctttgatcgc cggatgtggg tcatatcctt ctaattggat
    30721 atcttccatc tcaaaatcaa atactgactg cttttcctga ttcaaaacta aagtcggaaa
    30781 tgcgcgtatt tctcggctca attgttcttt catttgttcg atatgattga tataaagatg
    30841 agcatctcct aatgtgtgaa caaattcgcc aaccgctaaa cctgtttcat gtgcaattag
    30901 gtgagtcagc aacgcataac ttgcaatgtt gaacggaacg cctagaaaca catccccact
    30961 gcgttgatac aattggcagc ttagccgtcc ctcttgaaca taaaattgaa acatcgtatg
    31021 acaaggaggc aaagccatgc taggaacatc ttccggattc catgcagaaa cgatcaatct
    31081 tctagaatca ggagtctttt tgattgcttc gatcacttgt ttgatttgat cgataaagcc
    31141 accgtctttt gtttcccagt gacgccattg atagccgtag atgtgtccaa gatcgccgta
    31201 tgtttcagca aaggaatcat cggttaggat cttctcgcaa aaaagcttgc tctgcttttt
    31261 atattcttca ttgaattcag gatctgtcaa acatctatgg ccaaaatcag tcatatctgg
    31321 tccattatag tctggacttt tgatgtagcg ttcaaatgcc cattcatccc aaatatggtt
    31381 tttattttgt aatagatagc ggatattcgt atctcctttt aaaaaccaaa gcaattcgct
    31441 tttgatcaat ccaaaaggta ctcgtttcgt tgttaacaaa ggaaaccctt tttgcagatc
    31501 aaaccgcatt tggtatccga ataagcttaa tgtgcctgtg cctgttctgt cttctttacg
    31561 atgcccttct tccaacaatt ttcttcctaa gtcaagatac gcttgttcca tcctttttcc
    31621 tcctaatctt ctacccattc actcaagtat tcccaccgtg ccattttttc ttcaagttgt
    31681 gcttctgttt cagatacgtc gttttgcaat tcttgtaatc gagtgaaatt atctccttga
    31741 tgattcatct cttcttggag caatgagatt ttttcttcaa gttctgcaat ctcatcttca
    31801 atcgtttccc attctttttg ttccatgtaa gataatttct tcttttcagc aggttccttg
    31861 ctctgtattt ttggtttcgc tttttcagca ggctgctggg tctctttttg cttcagtaaa
    31921 taactggaca tgtttccgta ataaagttct tgttttccgt ttccttggaa tactaataac
    31981 ttatctgcta ccttgtccaa gaaataacgg tcatgggaaa cagtgatcac agcacctgga
    32041 aaacttctaa aataatcttc caaaatcgtc aatgttgcta tgtccaaatc attggtcggt
    32101 tcatccaata ataaaacatt aggttgctga atcagcagtt tcaacaaaaa taaccggcgt
    32161 ttttctccgc ctgaaagttt gcctatcagt gtcccatgca taaatcttgg aaataaaaat
    32221 cgttcaagta attctgctac tccgatttga ctgccatctg ctgtttttgc ttcttcagca
    32281 gcttcttgta agtaagcaat catccgttta tctggtgcca tttcttcatt ttcttgtgta
    32341 taatacgcaa gatgaaccgt ttcaccgata gacattgtac cgctatccag gggaattctg
    32401 ccagctagaa tattaagcaa tgtcgatttt cccgcaccat ttttaccagt aatacctagt
    32461 ctttcccgag attgaatcaa taaatctaaa tgttctagca gtgtttggtc atttatacgg
    32521 taactgccat ctttgatttc cagtactttt ttccctaaac gttgtgtcgc taggttcagc
    32581 tctaaatcat cttcttggtt tacttggtac aagttctcct ttagatcttc gaaacggttg
    32641 atccgtgctt gctgcttggt gccacgtgcc tgtacgccag ccctcatcca agctaattcc
    32701 tgtttataaa gccgcttgcg cttttcttct tgttctactt ctacacgatc acgttctgct
    32761 ttttctaaga cgtaagcttc ataatttcct ttatactcgt ataattttcc aaatgaaagt
    32821 tcgaagattc ggtttgctac tctatctaga aaataacgat cgtgggtaac catcaatagt
    32881 gcgccacggt attgtttcaa atagttttcc aaccactcga tcgcttcata atctaaatgg
    32941 tttgttggtt cgtctagtaa tagtaaatcg ggttcatcaa tcaacacttg cgccagacta
    33001 actcgttttt tctgaccacc tgataaagta ctgatttttt tatctaattc actgattcct
    33061 aatttttgca aaataatctt tgcgttggta tctgtagtcc aagcatcttc tttattcatt
    33121 tgttcttctg ctttagcata acgtttttgc acatcagggt cttctccgtt ttcccctagt
    33181 tcgatcaatg cttcctcata tgctcgaaca gtttggatca aggggctgtt tccttgaaag
    33241 actgcttgga gaacagttaa ttcatcggaa aattcagctg cttgagaaag ataaccaata
    33301 cggtaatcag ttggataaaa cactgtttgc cgatcaccgt cgctgctatc gataccggca
    33361 ataatattta agagacttgt tttacccgtc ccgttcgtcc caatcaaccc gatacggtct
    33421 ttttcgtgga ccaaaaaaga aatctggtca aacaaatctt tttcaccata agtttttttg
    33481 aggtcagtta cttttaattc tttcaacatg ttccctcctt atcttggata ttgtatcata
    33541 aaaatagtga acttcaggaa aatttttttg gtatttgttc tttttgtgtt ctcaaaaaaa
    33601 gaacaggacc gattcgatcc tgttctgata tgttttgttt tttataaaac ttgaaaattt
    33661 ttaccttttt attttattgg actcgttttt tttgactttg ctcgaaacgc aaatcggaaa
    33721 gaaaagttat cagatggttt gttcatcgta taagattcta acaaattgat attcctgcga
    33781 tagctgttcg cagaaaacgt cgtgattttt tcagcaactt cgtattcatc aatgattttt
    33841 ctttcaagat aataacctct tagcattttt ttcgtaaaca gactatcttg ctggatcatc
    33901 cacgaagaaa tcaaattccc cgagttcatt tttattcctt caagtttgcg ttgctggata
    33961 aaaaattgaa ccatcgtttc atcataaata tcagtcaagt tatctaaact ttcaataatc
    34021 aactgagtat ttttttgata tgtctctctg atttcttgga tatcttcatc actaataaat
    34081 gtctgacgat cagcatatct tctttgccaa aacattttcg gatggagaac gattcgcatt
    34141 aacctacgga caacaatgaa ccaaaagccg ataaaagaca aaatctggct tgtgatagaa
    34201 tgttcgaatc gagaaagaaa acgggagtaa aaacgataac cattagaaga aagcttgccc
    34261 gaacgataac tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct
    34321 tgtacttctt gcttttcatc atcggttaaa tcttccaagt acagatcacg tatccgttct
    34381 tgataatttt ctatcactgc ttcagtggct aaaaattctt ttgtattacg atcaatttcc
    34441 tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc
    34501 gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga caatgatccc tatgaccaaa
    34561 gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag accgctggta aaacatcata
    34621 ttattgattg aaaatggcaa aataaaaatc gtagccaaac tgactgttcc ctttacaccg
    34681 ccaaatgtta agatcaagat ctcgttcaac tgttttttga actttttact gccatctttg
    34741 aatacataaa aaagactgat agaaatgaat ctgatcacaa aaagcatgac agagattaat
    34801 acgataatcg ctaataaaag accgtttgga tataaaccgt ctccccatac tggcgagaac
    34861 acttgcgtca attcgattcc taaaaatata aatactaatg cattcagtgt aaaagcaata
    34921 gttgtccatg tactgtggga caagctcgac aattctgcat caaacacaga gattttccta
    34981 aaaccagaag cttggagaat acctgctgcc actgcagcaa tgattccaga tacatcaaat
    35041 gcctctgcca atacataggc tagaaaagga agcaaaagtt cgatcagcag ataccctgtg
    35101 acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc
    35161 aaaaagccga tcaaagctcc cccaatacta gaaactacta gactcatccc cgcgtttact
    35221 gctgaaaaag atccggtgat cagcgcacca agagcaaact ggaaggcagt aacaccagat
    35281 gcatcattca aaagaccttc tccttccaaa atatgcatcg cctttttagg gatattcact
    35341 cgtccagaaa gagaactgac tgctaccgca tctgtcggac ctaacgctgc tccaaaagca
    35401 aagcaggcag caagtgggac acttggcaac aggaatgata acgtcgcacc taccccgaca
    35461 agggtcaaaa tgactcctcc aaatgccaag aacaaaattg tcccgaaatt tttgagaatc
    35521 gaagaaatat ctgctttttc tccttctcta aataacaatg gtgcaataat cataactaag
    35581 aacaattcag gttcaaaatc gattgattgt ccccactctg tcagacctaa taccactcca
    35641 aggaaaattt ggatcagtgg agctggaatc attggcaaaa tgcggctagc tatgttcgat
    35701 aaggtgatcg ttactgcaaa tacaataact aactcagcaa attccatcgt ccatcccctt
    35761 tattttttat tttgtgtaag catttttaaa aattgttctt ttttctttaa aattttggtt
    35821 tcgactactt caacctgtgt ttggttcccc tctaattcac actgtacacg ttttgactcc
    35881 aaacgaagca gatcatgttt cagacgttcc aactgacgat acatcaatat atttctttta
    35941 cgcaacttac aaagtatttg acggcggtca tccatcgaaa gggcgccata gcgatcatgc
    36001 aacagttttt tttgttgttc aaaactctct ctcattttcc agatctccta tacacaaact
    36061 tccatcatta tagcaaagtt ctttttggat gaaaaatatt tcattgtttc atcgtttatt
    36121 gctagttaga aatttccata aatttttcaa cgcctcattc tattccaaat agtatcagat
    36181 atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa ggatgtttac tttcccattc
    36241 ctgcctttct tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac
    36301 agtcccttat tctgaataaa cggtgtggaa gagacagcct atcagtagta ataagacaca
    36361 tctctttcac agcctcattt ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac
    36421 atcttctaca cgttgatctg gattgatcac gatatacaag ttacctgttt tttcgctgat
    36481 ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat atgggaagta
    36541 cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat cttcgtattt
    36601 caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat gtgtcgcaac
    36661 tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga tttcaacaaa
    36721 ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat
    36781 atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta gcgctgtcat
    36841 ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact acctcagttt
    36901 agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac gtatctgccc
    36961 actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa tcaatttttc
    37021 tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt ctacgacggc
    37081 tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc cagtttcttc
    37141 tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta aacgatcata
    37201 ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca attcttctac
    37261 ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag ctccagtttc
    37321 tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt tagaacactt
    37381 ccacgatttc aaaaaggaac gaatctcatt ttcttttaga tccagttggc tgatcaataa
    37441 tgcccatgct tggctctctt ctttcaatgg gacggatggt aattcagcaa aacgtaaaag
    37501 tgcttcgcct tcttctttca aaccgggaca gcaaatgtag cactctgttt caataaatgc
    37561 atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca catttatacg
    37621 ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga aggtttcttc
    37681 ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc gtaaggcatc
    37741 ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt ctagatcttg
    37801 cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg cattgatcgt
    37861 aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat gatctggtct
    37921 acggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt gttcatcatt
    37981 ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg gaaaaagctg
    38041 tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg gctggttcag
    38101 caagatatca cgaacacttc cacctacaaa ataagcttca tatcctgcat tctctaattt
    38161 tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt tcatttttcc
    38221 aacctttcct gcgtgtttct tgtcctgaga taaagactca gttgcatggt gactgtttga
    38281 caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact cgtccttacg
    38341 ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa aaacttcagt
    38401 taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat caccaagttc
    38461 ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat aagtgtggtt
    38521 gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag ctaatggaga
    38581 gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca tactgtataa
    38641 agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact ttaacgcaaa
    38701 tcctagataa gagcaattga gaaaatcgaa tttccatgct acatttagga aggaatttct
    38761 aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt caaacgaaaa
    38821 acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt tggtcttgtt
    38881 tacttgaaca tcgctaatca tttagcagaa gggggagtct cagggatcac cttgatctta
    38941 agagctttat ttggtattga tccagcttat acgactttat taatcaacat cccattgatt
    39001 ttactaggag gaaagatcct cggtaaacgt tccttggcct atactgttct aggtacactt
    39061 tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa tttacagcat
    39121 gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc
    39181 taccgcatgg gtggaacaac aggcggcagt gatattatcg ctcggatttt agaacaaaat
    39241 tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt acttgcttca
    39301 ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta tgttttcagt
    39361 cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact tgtcgtctcc
    39421 aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg tgtgactttc
    39481 ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc
    39541 tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa agctttcctt
    39601 tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa gccacagaca
    39661 agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa agattccaca
    39721 tccagctgac ctttgtcttt tttagtacta tactattttt aactaattgt tatgttcact
    39781 tacccggtta cttttctttt gtgtctgcaa aaggatcttt cggttaactt catcagataa
    39841 tttcaaaaaa gcatccctat ctccatcttc tagtgcttga tcgatttgag cataaagcat
    39901 ttttagtttt gcctcttctt ctttttcttg aaaatatcgt tcaattaatt cagtatcttc
    39961 ttcactgact gaagaatccc aagaagaaaa tggattgtct tctaagatag atagatattc
    40021 tcttgtctgc caagcatttt cgaatataca ttccacatat aaaggctttt tccaattcaa
    40081 acggatttca tgaaaaatct ggtctgtgtc cgtaaattct tttccctgca aaaacaattt
    40141 catgggttcc ccttcaacag atagatcact gatctgcagt cctcgatccg caaaattcgc
    40201 cccttcaaca aagtggacat tgcttaaaat cgcttcatga ttggcaagat aattcaagat
    40261 ccaaaaaact tctcttcggc taaaagatac atgattgacc atccacgtta aaaaattctt
    40321 cttttcccgt acatcaatta gcatttttct cacctctctg ttaggtcatc taataatgac
    40381 agtgcctcca tatctcctgg ttcaagtgtt aaataataag tcaataattc ttgcgctctt
    40441 ttcagctgcc cttcttctct taagaaaaag gcatactctt tcaagaaatc tggttcatgt
    40501 gatagttcat gataagcctg ttcatagtgt acagctgcaa cggtaaagtc ttctaattca
    40561 ttataagcat gggctaggtt ccattctgca taaggattag cagtttcttc catctgattg
    40621 atcacttgta tcacatcttc atatctttca tcatccaagt aaagattact taaagtaagt
    40681 aatgtttcat cttgtttgtc tcctacttct aaagctttca gtaaaagttc ttctgctcgc
    40741 tgtctatcat gcagacggaa agcattttcc gaggccaggt gataaagttc gacttggtaa
    40801 ggattttctt tgattccttc ttctaacact gtttgtgctt cttcaatcat ttcttcttct
    40861 tgaagagctt gtcctagata tagatataac gattgataat gcggattaac ttctcgaagt
    40921 tcttccagat agttgattgc ctgctgattt tctttcaatt gaagattcgt aaaagctagc
    40981 tggaacagtc gatcatctgt cctttcttct ttcagtgctt cttctaaagg tacgatagct
    41041 gcttcgaatt ctccttgcat acttaaggat tgtcctaaac gttcctgcat cgagattcct
    41101 gaaatttcat ttatcccgct tgccaacaat gtttcataaa ccactgctgc ctctttgaaa
    41161 cgatcaactg aaaaatacaa ttcagctagc gcaaactgga tcaatggttc atcaggaagc
    41221 aacccagctg cttctttcaa ttttgcttca ctcacttcag ggattccgat aacttggtac
    41281 aaatcagcta tcgccaataa gctttgcgga tagtaatcac tttgcttatc cactttttcc
    41341 aggtattcaa aagctaagtc gatctcatta tcttcgattg caatttctgc taacgggatg
    41401 ttcagtccgt cattttctgg gtattggttg atcagttgtt caaaaatctg ttttgcttcg
    41461 atcaaaaagc caatagccag caattcttcg cctagttcag ctaatatatc tgctgggtct
    41521 tcttttaatg ctttttctaa aaagatttgt gcttgtgaca agtcttcatc tgataaagct
    41581 tgcaacattt tttcgctatt tgtattcatt tcaattccct cttatctcac tgacgatttg
    41641 ttgaaatacc gtcattgttc tcaataattc tttttcgttt ggatgttggt atccgtctat
    41701 tatacctatt ttttctaaaa caagtatttt tttgcttttt tctgccaaaa aaagaacttt
    41761 ttccaaatac tccatctttg aaatctgttc cagccagtgg attggatagc caagcaagtc
    41821 cagccacaca aaaaagtttt ttagttggaa atcaaaagga tatgcttgga gattccacac
    41881 gaaatgaaac atcattccta aaaatttttt catactagca gaaagtagat ggccattttc
    41941 ggtcagataa aaagcttgtt caaacagttt accataattt gacaggtttt ctgcctctaa
    42001 ttgataaaaa tagatcattt cttcgatcat cccggcaaac ggccgagtcg ttaattgctt
    42061 tttatttgga tagtttttat acagattttg caaaaaacga tgatcacaaa tcattccgca
    42121 acagataaaa gtcaaaaagt caatctgctt tccgtccacc tgccgctcgc tgattgtttg
    42181 atcataaacg atgcgttctg gcagattgat ggtttgcagc actacacgat ttggtagctg
    42241 caaaatcgtc cgctgcggtg ttaatgctcg gcttagagaa cggatcgata cagggatcat
    42301 ccatagtgat gaatcaaggg ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc
    42361 ttcatttcca aatccgatga taaacatgga ttgattttcg ggatagcgtt tgacgaaatc
    42421 aagcaggttc tttaattctg ttaaatgatt gcattgagtg ttggcacaaa tataccagtc
    42481 attgtttgct tgatttgcaa ataacgggtt gatcttatct gcaaacaaat cataatagcg
    42541 ctgattcgtt aaaaataaga ttggctgatc aatggaaagt tctcttagct gactgactag
    42601 cgtttctccg taaaacactt tcgtcaacgc atcttctact tgataatcaa gttccatacg
    42661 catccctcct cttcttattt ttattttacc attagtaagc aaattctaga aaagataaga
    42721 gcaaagctca cctcataact tgcaaataaa taaaaattaa ataaaataca tgtgttttct
    42781 atgtatgaca tacggattcg gaatctctgt ctcttattta ttccttttct gctaggaaat
    42841 aaaaagaaaa gcacttgtag ccctagagcc acaagcgctt tttcaactgt cttatttaac
    42901 agcgtctttt aatgctttac ctggtttgaa tgcaggtact ttgctagctg gaatttcgat
    42961 ttcttcacca gtttgtgggt tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt
    43021 accaaaaccg attaattgaa ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa
    43081 tacagcatct actgctgcag ttgcatcttt tttagttaaa tcagtagctg aagcaacttt
    43141 ttcgattaat tctgctttgt ttgccatgga tagattcacc tcctccacaa gagatatgat
    43201 agaaatatca tttagttaaa actttctgag tggtccagaa gtttaaaaag aaatattgat
    43261 taacttcaat attctgttat cacgatatca tatgaaccgc ttaatagcaa ggtttttacc
    43321 aattttcaca tgcttttttt agtcatagaa acgtaacaaa cgaaactgtc tttttctttt
    43381 gaaaaaaaaa cgtcgatatg gtaaaatgcc tattttctcc tacgagggat aattcggatg
    43441 gacgtccctt caaaagtaaa tgcttttctg atttgatttt ctaagaaacg ttcataagaa
    43501 aaatgcatca attcctcttc attgacaaaa acgacaaatg taggtggttt gacagcgact
    43561 tgtgtagcat aaaaaatctt caggcgtttt cctttatctg ttggtgttgg attgattgct
    43621 acagcatcca taatgatatc attcaacaca gccgaagaga tacgaagatt ctggttcata
    43681 cttacttgtt cgatcatttc aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca
    43741 aaaacgattg gagcgtaatc aagataccgg aattcatcac gaatctcttg ttcgaaatca
    43801 cgcattgtat tggtttcttt ctttacaaga tcccatttat tcacgacgat aataattcca
    43861 cgaccagctt catgggcgta acccgctact cgcttgtctt gttcccgaat cccttcttca
    43921 gcattcagta ccatcaaaac gatatcagaa cggtcaatgg cacgcattgc acgcatcacg
    43981 ctgtattttt cagtattttc gtatactttt ccgcgtttac gcataccagc tgtatctatc
    44041 attaagaatt tctgaccact ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt
    44101 ccttcaatat ccgaaacaat tacacgttct tctcctaaaa tcgcattgat caaagaggat
    44161 ttaccaacat ttgggcgacc aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca
    44221 gtctcttcag agaaatactt cactgcttca tctaaaatat caccaatccc tagtccatgg
    44281 cttcctgaaa caggataagg atcgcctagc cctaaggaat aaaattcata gatatcgttt
    44341 ctcatttctg gattatctac tttattgact gctaaaatca ctggtttatt acttctgtat
    44401 aaaattttcg caaccatctc atctgcgtct gtcacgcctt cgcgtccgct gactacacaa
    44461 atgattacat ctgcttcttc aatcgcgatt tctgcttgat gcttgatctg atccataaaa
    44521 ggttcatcgc caagatcaat tccgcctgtg tcgatcacgc tgaattctcg acctaaccat
    44581 tcaccttttg cataaatacg atcacgagtt actccaggag tatcttcgac aatcgagata
    44641 cgctcaccag cgatgcggtt aaaaatcgta gacttaccga cgttcgggcg gccaacgatt
    44701 gcaattgttg gatttgccat attatttcct cctcacaagt ttccaatctc acttagttta
    44761 acgaatgcat tgccaacttt caagcataga ttgtcaatta aacgaagtat ttttattcct
    44821 tattgaaata ctaatcgctt ttcaaaagaa aaactgagac aaaagcagct gctttcatct
    44881 cagtcacaga tacaaaatca attatttttc agaatcatcg gttgcttttt cttctacttc
    44941 ttctttcaac gcatcaccta aaatgtctcc catagtaaat ccagtatttt cttctggtaa
    45001 ttcataagac tcaacatttt ttggttcttc ttctgaagct ggtttttctt ctaaagcttt
    45061 gatactcaat gcaatacgat gttcttctgg atggatctct aatactttca ctttcacttc
    45121 gtcaccttca tgaagcactt catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat
    45181 atggactagc ccttctactc cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg
    45241 tttaactgtt ccgtctaata ctgtgccaac tggagctttt gtatcgattt ctgaccaagg
    45301 tccaggctgt gtgtctttga ttgacaagga aacacgttct tgttcaggat tgatagacaa
    45361 cactttgaca tttacatcat cattgacagc caatacatca cttggttttg ctacatgact
    45421 atgtgaaatc tctgagacat ggactagacc gtcaattccg cctaggtcga caaatgcgcc
    45481 aaaatcagta agacgtgcaa cacgtccact gagaacatct ccctcatgga tagttgaaag
    45541 aatttctttt ttcttttctt ctctttcgtt ttccactacc gctttatgcg ataagatcaa
    45601 acgattttct gatggttcga tttcgatgat tttaaagctc aatgttttgc ctttatagtc
    45661 tgaaaagtct gcaacaaaat gatcttccac cattgatgca gggacaaaac cacgtacacc
    45721 gacgtcaacg actaaaccgc ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc
    45781 ctcttggaaa tctctttcga tatcttccca aactttttta gcatctaaac ggcgttttga
    45841 aagtagatag ctgccgtttt ctttatcttt gccgatcgta ctgataacga ctaaatctaa
    45901 tacatcaccg acttttacta cttcgctgat atcttctacc ggcaaagttg ataattcttt
    45961 tgccggaacg actccttcaa ctccagcccc ttcgattccg acaatcactt gtttgtcttc
    46021 aatgactaaa acttcgccct taacaacatc gccaactttt acttcttgta cgcttttcat
    46081 tgcgtcttcc attgactggt tgttctcttg attttgatca aattctgtca taacacttgt
    46141 cctcctaacc aacattctgc cgtaagtacg gtactgtttt tagtttaatt gattattcaa
    46201 aaaaaataaa gcgatttcgt tcgttttcct tgaaaaaaca attgtttttc attttttttc
    46261 ttaatttaaa aaagaagata accttttttc aaaacaacag cttttattgc tgcaacgact
    46321 tctggaatcg atttgccagt cgtatcgata cgaacagcat cctctgcttg cttcagcgga
    46381 gagacgtccc ttgttgaatc ataatggtct ctttcctgta tctctttcgt cagtgtttca
    46441 agatcagtcg gaattccttt ttcttggttc tctttgaaac gtctttctgc acgctcactt
    46501 acgcttgcta ctaaaaagat tttcacttct gcattcggta atacagctgt cccgatgtct
    46561 cttccgtcca tgacaacgcc accgcgttcc cctatttttc gttggacagc aactaattct
    46621 tctctgactt ttccatgtgc agagacttca gaaacagctt ttgtgacatc tggttgacgg
    46681 atttctaaag tcacatcttt tgatgctatg aatacatgct gtccatcttc ttgttgcgaa
    46741 aaagagatag gatacttttt gatcagcgaa actagccctt cttcatctga aaaagataca
    46801 ttgttcgtaa tagctaaata agttactgcg cgatacatgg caccggtatc tgtataaata
    46861 taattcaatt cttttgctaa tatcttggca acggtacttt tcccagatga agcaggcccg
    46921 tcgattgcaa tactgatttg tgtcatgata taactccttt ttctcagaaa aaaagccgca
    46981 aagcgcggct attattattt aattcttaat tcttgaccag ggtaaagcat aaagttgttt
    47041 ggatctaagc cattcaattc aaacaattga tcagtggtga tccctgcacg ctcagccact
    47101 tgttttggac cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa
    47161 ggtgttgttt ctgctgcatt gctgcttgaa tcccctgctt gataagttgc ctcacttgag
    47221 gaagtcggtg ttgtctctgc tgcaatactg ctttctgacg ttacacctgt atcttgagac
    47281 acagtagatt cttccgcgat tgtactagat tctactgttg aagtctcagt tgactcctct
    47341 tttgttgtac ttgtagaact ttctactact gatgtcgaag tttcagaaga agttgctgct
    47401 gttgcattgt tgcttgatcc atttctgatc cagaaataag cacctgctgg aatagcgata
    47461 cataagacca gtaaaatcaa aaatagcgaa agaaataatg tatttccttt cttttgttgt
    47521 ctctgagaac ttcttgatga aacttcatca tctgtatcgt agattggttg ttcccatggt
    47581 tcttgtgttt cgttattttc tgtagattga tgtctatctt ttctgctcac tgttttacct
    47641 cctaaactct atcgtgtgta ttataccata ggaaaaagtg tcttgtccta aaaaatcaat
    47701 gttccatgtt ttttttaaat aaatttagta aaatcgtttc ccaatgaagt tgttcagtct
    47761 tagaaaaaag cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat
    47821 tctgcggctt ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt
    47881 tttcttcatt aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac
    47941 gtttgagccg ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt
    48001 ttttttgcag ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac
    48061 tttgtcgctg ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt
    48121 gataaaaaag aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta
    48181 catagttttc tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat
    48241 cgatccccat accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact
    48301 gctgttgaag ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa
    48361 aacgttccct gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga
    48421 ttgccgctcc ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt
    48481 tccgaacaaa aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc
    48541 gatcaaatag tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg
    48601 ccagtgtgac cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac
    48661 ggaaatcaac tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat
    48721 tttgtttttt cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca
    48781 aaaatttata ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac
    48841 tattgaaagc agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca
    48901 ttagagaaag cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt
    48961 gatagcaaag gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa
    49021 gcaacgcctg aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt
    49081 ttaattcatt tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa
    49141 cgaaattctc cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc
    49201 acggaatcct tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat
    49261 ggaaaatcat caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt
    49321 aaatgacgga ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg
    49381 atcatcatgc tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc
    49441 gaatctggcc gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga
    49501 taaaccgctg actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca
    49561 tttccagata attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc
    49621 tgcaccaaac tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta
    49681 agccattttt ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga
    49741 atacccgttg ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt
    49801 tcccctgttc tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac
    49861 aacatatcag cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct
    49921 tgctgcgcaa ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg
    49981 ttatggataa acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt
    50041 cccacaagga gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat
    50101 aaaataaaaa caatcatatt attctcctta agttaggatg gttagaaatg tcactactat
    50161 gtaaacttgt acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata
    50221 ttttcgatta tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac
    50281 agttcgtgat gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc
    50341 gagcgatttt attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa
    50401 gttctctaaa cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt
    50461 attttgaaag attcatttgc tatctatagt taattgacta gttttttcac ggaccctata
    50521 ggacccattt atattatttc aactttttca gccagttttc caatgtattg acgatcacag
    50581 tttgctggtc tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc
    50641 caaaacctcc atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact
    50701 gcttagcttc ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata
    50761 aaacaggaac agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga
    50821 ataccccttt taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc
    50881 cacctaatga atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat
    50941 ccgctttgtt tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa
    51001 agccgttttt tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac
    51061 caggataaaa aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata
    51121 agtaatcttc tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt
    51181 acgtattttg ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta
    51241 agccaattag tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc
    51301 cctttttctt aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc
    51361 cagcatcaaa taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta
    51421 tttttattta gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat
    51481 actacttaat tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat
    51541 cgcaccgaat gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa
    51601 cggttcagtc atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc
    51661 actgtctagt ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata
    51721 tttttctgct gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat
    51781 tctcatatct acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat
    51841 cgttagctcg ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt
    51901 ttcttgaact aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc
    51961 aattgccaga cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt
    52021 accaagaacg acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc
    52081 ttcttttact tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa
    52141 cgctcctcca gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa
    52201 taagcctttt tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat
    52261 tccttcttct ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc
    52321 aaagataaaa cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc
    52381 atacattgcc gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc
    52441 gtcttttata gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac
    52501 aacgtccata tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc
    52561 gtaatagcct tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac
    52621 tttatccaaa gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc
    52681 atctagtacg gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg
    52741 ttgttctgtc acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt
    52801 caaattcctc cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca
    52861 gcaagcagaa tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg
    52921 acttatccac gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt
    52981 gtacctgtat ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct
    53041 acaaataata tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga
    53101 gcaattgcta cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca
    53161 gatgcagatt gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata
    53221 ggtcccatga ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac
    53281 atagggatat agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat
    53341 ccttgttcgc cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg
    53401 ataccacgag caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa
    53461 aattcagaga caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt
    53521 actaagaata acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta
    53581 cctagaaccg tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc
    53641 gtgaaattcg cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta
    53701 ataaagaaca atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt
    53761 gcttcaatat cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt
    53821 ggatcttttt cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa
    53881 ataacgaaca tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt
    53941 gaagctacgc ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct
    54001 actaatacga ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc
    54061 agtaaagcat agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca
    54121 aataacagca tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact
    54181 gaagcaatcc cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca
    54241 accaaaatga agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct
    54301 ggtacaccta gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt
    54361 tgttgtactc gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc
    54421 ccagctggta taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta
    54481 tacgctgaag gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct
    54541 tttccgttag agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac
    54601 aaaaataata acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc
    54661 ctcctctttt ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt
    54721 tataaacaaa cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat
    54781 tgatttttat gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat
    54841 tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata
    54901 tttatttgac tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa
    54961 gaagttgcgc atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga
    55021 aaggaacgat cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt
    55081 tcagccctaa aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa
    55141 aaatagttag agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct
    55201 gtttctttac actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac
    55261 tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg
    55321 aacgcaagaa cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata
    55381 ccgagatatc actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa
    55441 aatactgtaa aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac
    55501 gggtgttctt catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt
    55561 tctctccaaa tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa
    55621 gcgtcgtcaa ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca
    55681 ccgattcagc tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc
    55741 accctgcccc tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct
    55801 attcaaaaaa cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag
    55861 caaactgctg acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa
    55921 agtcagatct ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag
    55981 catatttttc acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt
    56041 tgtcttagga tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat
    56101 ctttattcct tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc
    56161 aacgtaaact ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc
    56221 atttgtcagt agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg
    56281 ttcttttacc atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc
    56341 agaaataact cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg
    56401 gacttcatcc acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac
    56461 ttgtccgttt acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt
    56521 gatgccagcg tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc
    56581 attccatcaa agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt
    56641 gtatcaggca gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt
    56701 ccatataata tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg
    56761 gcagctagac gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct
    56821 cttgtgattg gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat
    56881 gtgtttgatg tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt
    56941 aatacataat ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa
    57001 gattctttta attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg
    57061 cttaattctt cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca
    57121 cttaatttcg tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt
    57181 ttctttttgt tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt
    57241 agtgacaact tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc
    57301 atttcttttt atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg
    57361 cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc
    57421 acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa
    57481 cccatcgatg ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt
    57541 cagtctttca gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc
    57601 tcgaggatct tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa
    57661 tgtcttgctt ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc
    57721 tagctcgagt gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat
    57781 cggtatatca tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg
    57841 accctcgaat acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt
    57901 ttctttgcta gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca
    57961 tccgggtact tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa
    58021 gacggattca aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca
    58081 ataatccctt gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct
    58141 tcagattcgt acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg
    58201 aaactcaata gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc
    58261 tcaccttcct atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc
    58321 ttcgtgatat gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt
    58381 actgtccgca aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta
    58441 tgtggtgtta cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag
    58501 attccttgcc gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg
    58561 tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa
    58621 tcacctaaag gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga
    58681 gaaagatgaa gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca
    58741 tacatgactt ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct
    58801 tcaatcagct tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc
    58861 ggcgtatcga tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga
    58921 tggaatcttc tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca
    58981 tgcaacttct ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt
    59041 ttctgctcct gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta
    59101 ttggcggaca atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc
    59161 ataataatcc ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt
    59221 ttccataatc tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt
    59281 ctttttcata gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa
    59341 aatgataacg ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct
    59401 cgatcgtgcc gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc
    59461 tcaaatcata acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg
    59521 tcaagatttc aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca
    59581 ctaaaaagaa gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca
    59641 gagtagcttc tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt
    59701 ttttcatcgc cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt
    59761 acaaataaaa aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa
    59821 acatgcaagg gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt
    59881 aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc
    59941 aaaagctttc atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc
    60001 agaacgttct aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct
    60061 tggcttcaat gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat
    60121 ctgctcgcct agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa
    60181 atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc
    60241 tttgtttttc atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc
    60301 taaactgccc caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc
    60361 ttttttaggc catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt
    60421 atctggaaga ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc
    60481 aaaaccgtaa tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt
    60541 ttgattttgg taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt
    60601 gcttaagcca aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat
    60661 aataatacct gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga
    60721 aaggttttct taatacattt ctttgctatc ataacataat gttatctact tgaatagctg
    60781 agccttggtt tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat actccccttt
    60841 ttcgaataaa cggtgttaaa cggcaggctc acagcctctt tcatttgcct aaaattaatt
    60901 gctcccattt cctccgaacc atccagtttt acagttgatg gttacgatgt aaaatggcaa
    60961 agcactttct ccaatgcctt tcatcaatgc agattttgtt gaagtggcat atagattata
    61021 atagccttcc ccattttcga ttctggcttt ttctaatctg taacctcctt caacgatata
    61081 tccacgttgg atacatgtag caatcacttt gctttggaca cctggtgccc acgtccatgc
    61141 tggattagat gcatcggcaa tatcacttgc tctggcagat ttgattggct gattcgctga
    61201 gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc
    61261 ttgttgagcg gcttgagctg cagctgctgc ttgagcggct tgttttgctt gttcttcagc
    61321 tgcttttttc gcttcttctg cttttttctt ttcctcttct tcttttttct ctttatcagt
    61381 gagcatctta tctacttgtt tcaattgttc attcaactga gcagatagat ctgtattttt
    61441 cactttttct actgcttctt ttgccgtctt gtattggtcg cgtgttgctg aatccactac
    61501 ttttccgtct ttaaagacta tagctgtttt ttctttagct gtttgcaatt cagcatattg
    61561 atttttagct tctgtttgtg cttgttgaat caattttcca aaatctgtgt tatccgtttc
    61621 gattgcttgg atctctttat ctgctttcaa cttgatgtca tctgctagat gatcatcgac
    61681 gatggcaggt gctatgaacc actcattcac tttttggata tctcggatct tagattggat
    61741 atcttggtaa gttgtttcta actctgaata tcctttttca ttcttaagac tggataaatc
    61801 attttttagt tttgataaat cttgatgaat catatctgca cgaataaatt gatggtgatc
    61861 atctgtataa aaggcagcaa gtttctcatg aagttcttcc atttgttgct gtctttttgc
    61921 ttctgctgct acttcggctt ctactttttg atgatggtca taataagccc agcttcctcc
    61981 accgagtaga agtactgctg ctaaaacgag ataaggtact ttttttgatt ttttcttttg
    62041 cgctttatct ggatctgttt tttctaagtt tgattcttga tgtgtttttt cagtatctgg
    62101 cacgattgtt attgatttcg cttctgcttc aaacagctta ggctggttct cttctttttc
    62161 tatttttgct tcaagatcag taccctcttt gcttacagat tgattttcag catcggattc
    62221 actatttatt tcatcgggct ctgttgactt tttgtctgtg ttttcgagta tttctttttt
    62281 ctctgcttcc ggcagtttat tcgttttttc cttttctaat tgtttttcag taaatacttc
    62341 agttttttct gtgatatccg tttttacgtc ttttattttt actgtttctt tacctgtggc
    62401 gttttctcta tcagagtctt tttcagtttc ttgttccttt tctggctcta ctttgttttc
    62461 ctgtgcaatt ttttcactga tcattttgag cagctctttc gcgtgctctt cttttgtatc
    62521 ttcacgatgt gctttgatat aagcggatag tatcgaattt tcttcctcat tatcgacgat
    62581 ttctttcagt tcagctactc ttcttttttg ttttgattca agcgtttctt cttgattctt
    62641 tcctgattct tccgaagtct catgaacagt tgctttcttc tgttcatttt tctctatatt
    62701 agttacttga tcgtgagcag gtggttcttc gtcttctggt tctgcttttt cgaataattc
    62761 cattacagat tctagaggta aatctttcaa ttccgaccat tcgattggat cgtttaattc
    62821 tgtatcagga aagactgcat cttcagttga tttatcctgc atagctgcat ctgtatttgt
    62881 ttcttcaatt gattctttta tttgctgatc ttctgcagtt atttgatgac cgcagtctgg
    62941 acagatagta ttctgctcca tttttttcga tccgcaattt ggacattttt tcatcactta
    63001 gcgatcggct cctttactag gtttacgcta ccatttaatc tatcatattc tatacgtcac
    63061 gccaatagct tatttttagg atttacttaa aaataatgaa tgaatttcct tcctatagaa
    63121 aaaaaagctt gagactgcgt agtctcaagc tttttttctt ttaaaataaa ccaaacagtt
    63181 ttactgattt ctcttctggt caatagtcca cgcttattct aagaaatctt ttaattgttt
    63241 tgaacgagaa ggatggcgca attttcttaa tgctttagcc tcgatctgac gaatacgttc
    63301 ccgagtaaca ccaaacactt tccctacttc ttccaatgtt ctcgtccggc catcatctaa
    63361 accaaaacgt aagcgaagga cattctcttc ccgatcagtc aatgtatcta gtacatcttc
    63421 caattgttct tttagtaact cataagctgc gtgttctgct ggacttgttg cttcctggtc
    63481 ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg attggcgttt caagggaaac
    63541 aggttcttgc gcgattttca agatttctcg gactttttct gttggaagat ccatttccgc
    63601 accgatttct tctggcgtag gttctcttcc aaggtcttgc agcaattgac gttggatacg
    63661 tatcaattta ttaattgttt caaccatatg aactggaata cggatcgtac gtgcttggtc
    63721 agcgattgct cgagtgatgg cttgacgaat ccaccatgta gcataggttg aaaacttaaa
    63781 tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt cccatgtttc cttcttggat
    63841 caagtcaagg aactgcatgc ctcgtcctac ataacgttta gcgatactta ctactaaacg
    63901 aaggtttgct tctgccagac gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc
    63961 taacgctact tcttcttccg cagttaaaag ggagacacgg ccaatttctt tcagatacat
    64021 acgaaccggg tcattgattt ttacaccggt tggcgcagaa agatcttctg tttttgcttg
    64081 ttcagctttt ttctcagcac tcttcaagct gtgaattgat ggatcaccat tttcatccac
    64141 cacactgata cctgcgtctt caatcttttg gatcaatttg tccatttcat ctgcattcaa
    64201 agtgaatggt gtagcaagct gattggataa atcatcatat acaacttgtc gtttgggttt
    64261 gttttctttg atgaaagctg ctactgctgc ttcatacgtt tttttatctg ttccatttgc
    64321 cataaaagaa ggcccccctt gagtagctat ttggcttgtt ttaactgttt cgttaaattg
    64381 atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc
    64441 tgcatttgtt tttttaatgt tatttcatca gctaagccag atttgctgat cacatctagc
    64501 aagtcttgga tttctcgtgg cgaactttct tctgccaccg gtaaatattc gatttccacc
    64561 accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa actctgcaag gttgaattca
    64621 taatgttgtg agacatatgc atcgaataaa aaataaactt ctgcatatgc atcatgaata
    64681 aactgaaatt ctcgttcttt taaaatatta cggacagaaa cttctttaaa tgcacgatat
    64741 aatagaatct gctctgcctt ttctacttgc gtcaaaggtc tctttttccg tgcagtttct
    64801 tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt gacgattttg ctgatttgtc
    64861 cttcgaagat cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa ctctgttgac
    64921 agttgagata gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt
    64981 aattcttcga ggtattcgat cttgtctttt tcattatcta aatttttccc ttgttttaaa
    65041 taacgtgatt taaaagtaaa gactgtttcg cgcccatgag ccactaattc ttggaaagat
    65101 gcttctccat acttacggag atattcatca ggatcaagct tttctggaac gactacgaca
    65161 gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa
    65221 cctgcgttgt ctccgtcata gcaaaagacc aattctttcg tcagcttttc catttgttgg
    65281 atctgttgag ctgttagact tgtccccata gaagctaatc cagttttgat accagcctgc
    65341 catgcagcaa tcacatccat aaacccttcg aacaacaaaa tttctccgtt tttccggata
    65401 tcgcttcttg ctttatcaag attaaataaa acttgtcttt tattgaacag ttctgtttct
    65461 ggactattca agtatttggg ctgatctttt tcctgccctt tttcaggtgc aagccagcgt
    65521 cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac tctttgataa
    65581 aaacgatcag acatacggcc atcatcatgc gtgacaaaca atccagagcg ttctaacaat
    65641 tcatttgaaa ttgattcatt cttgaatact tgctgaagaa aagaacgctc attaggtgca
    65701 aaacctatgt tgaattcttc aattaattct aaagacaacc cacgactttg caaatattct
    65761 aaagctggct gtccagcttt tgtatgcaat agcatgtgat gatagatttc cgctgctttt
    65821 tcatgagcag caatcagttt ccccgtagca gattcaggat tttggacagc tatagtacgc
    65881 tgacgccatt gatcttctaa cggtatctgt tcgaagtctg ccacttttac cactgcttct
    65941 ggaaaactta gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa
    66001 ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt acgttcttca
    66061 tgaaaaggac acagccccaa gtaattttta ccagactttt tcaactggac atattgtcca
    66121 atcacctcaa cgatattcgt ttgactgcgt actgtttcga taacctcttg aggaatccgt
    66181 tgagccattt tttcaccccc tgctctattg atatcttttg ggtttaacag aagatatttc
    66241 aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta aaggacacaa
    66301 ttatacatgt ttgattgtat catatttcat tgctttttca agtataacac atttctatct
    66361 cattaagcat caatttgcta ctcttttcag taactgattt tgaatatcag ccttccagta
    66421 caatactcga agaacaatat ttacgagcat gaatacaaag aagtatccta cgctctctcc
    66481 caaccctaag tttacttcca aaattccatg gaacagcatt gcagctacat atatccctac
    66541 gatcgtcgtt actgctagga agatgcgcaa tgggttgaag ggataacaag cttttacaac
    66601 tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa gtagtagttt cagcatttcc
    66661 ccatgcgaat gtttttccaa gtagatacac cgcaatgata ttcactacta caagcaaggc
    66721 atttggtaaa gcattaataa gtgctgtagg caaataacgg taagtgattt ttcttttatc
    66781 ttgttcaaaa gataaaaaga aggaaggata cccttcaatc gctaaatcaa tcagcgtgat
    66841 ttggataggg ataaacggaa atccaattgc tgtaacggca catataatcg ataaaataaa
    66901 tgaataaatc gttttgataa aaaagatgcc tgagactttc gtcacattgt tgaccactcg
    66961 gcgtccttca aacaggactt ctggcaatgt agtaaaatca gagtccaaca ataccagatt
    67021 cgcaatctga cgtgttgctc catcaccttc tgccatagca atgctgcaat ctgcttctct
    67081 taacgccaaa acatcattca caccgtcgcc tgtcattgca accgttctgc cgctttcttt
    67141 caattcattt accagcaatt tcttttgctg cggactcact cgtccaaaaa ccgtatagcg
    67201 atgagcagct tctcgcactt ccatttcttc agtaatttga gaaagatcga catacgcatc
    67261 ataattcggt aatcctgcac gtctagcgat attggagaca gtaaaaggat tgtctccaga
    67321 aatcactttc agatccacac cttcctcatg aagataagct agtgtttctt gagcgttttt
    67381 tcggattgga tcatcgattt caaacagagc aaccggctca acaaatgctg gtttttcttc
    67441 gttcaattgc tgatttggag cgatccccaa cataagtacg cgatagccat tcgcttgggc
    67501 tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta
    67561 cacgactccc agttctggaa attccatcgc gccccatttg cgatcagaag aaaatgcgac
    67621 tttgtttgtc acatcatacc gattagaaag ctcaaaataa tctctcatag cccgcatagt
    67681 gatgttgtta tcactgcttt ctgccagata cgtccccata atttctggga tggctttctc
    67741 gtacagcgga tgtagtgctt ctacccgctg gatactcatt ttcccttcag taatggtccc
    67801 tgttttatcc aagcataatg tatcgacatg ggccagcgtt tcaattgaat acatgtcttg
    67861 aacaaggatt cgtttttttg ccagtttggt tacccctgtc gttaatgcaa tacttattag
    67921 caataccaaa ccctttggaa gcatgcctaa caaagctgct gcagatgcaa taacagaaat
    67981 tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat
    68041 aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt cagaacgaat
    68101 agctttgtga gtctttgcct cgctagtcaa gcggacagca taattatctg cacctactcg
    68161 gataacttca gccatgacag aaccgcttgt caaaaagctt ccagaaagca attcagctcc
    68221 agtttctttg acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg
    68281 tccagatata acctgtaaat cggctggtat ctgttctcct gctccgattt ttaccaaatc
    68341 tcctaatacc agctctttag tagaaatcga ttgttcttta ccgtcccgga tcactagtac
    68401 ttctgattgg gaaacaatcg ataatttccg tacaagattt cttgcatgta tttcttgata
    68461 aattccaata acgatattga cgacaataat tgctaaatac gccatgttgg aatacgcacc
    68521 tacaaataac agacaaatcc caataccaaa gttcagaaaa ttgaataacg tcaatatatt
    68581 atcgctgaat atttgttttg tactttttga tgcatcttcc tggtagtcat tttgctggcc
    68641 agaagccatt ttctgaacta cctcttgctg tgtaagtcca ttcattttct ttttctccat
    68701 tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca agattaaaaa
    68761 caacttgttt ttataaaatt tatagaaaaa cacacgaatg attttacaaa aaagatgcca
    68821 acctttttgt ttgaatcacc cgtgtgctgt ttttattatc aatctatctt tacttatatt
    68881 gttcctccaa acggctcatc caccaaccta gtgctgtacc ggctataaaa agaacaggac
    68941 aagctagtac cccagcaact cccaatcccg cgtaatcggt aggaatgatc ataatcgttg
    69001 aagcaaaaac tacacctaaa atgaaatgga atagcttgct atatgccttt gagaaaatgt
    69061 aatccatgat ttttgacaat ccaagaacac aaagaatccc accaattccg atcggcacaa
    69121 tcacttcaag tcgtacttct ttaaatccgt cagccatcgc tttatacaat cccatataca
    69181 ccaaaaaatt agaagggctc aatcctggga cgatcatacc caaaccaatc aatccgccgg
    69241 caatcagcca tgttccaaaa ttctgatcga cttgcccgtc aaataattga gcgccgtata
    69301 ataaaaacaa ataagccaga acaaaagtaa caaccatgat gatcacatca gaagtatctc
    69361 ttcctttttt ccctgcttct ttccataacg caggaacagt tccagcgata catccgacaa
    69421 aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa actgactaaa aaagaaagta
    69481 gaaagatgcc tgtcactccg cctagaccaa caggcagaaa aaacaaaaca ttttctttga
    69541 aattttttgt aaggtgggct aaaaaagaaa tcagccgctc atacatgcca aagacagctg
    69601 ctaatgcacc accgctgact cctggcaaga taaatcctga accgataaac attcccttca
    69661 caaaccgtaa catccagtct ttggttgtgt accccacttc ttgattttcc attttttgcc
    69721 ttttttccac tatttgcgca ctccttcttt catttaattt tattcatgat tgaacatttc
    69781 ctgctatttt acttaaagac tttttcatag gtctctatta ttttttcatt cattttgaca
    69841 atatcaggat aaaacatgtt acctccatta tgatagagat agccgccatt gtaaagcaag
    69901 gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag tgggctcagc
    69961 acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg attttttaca
    70021 taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa atcgcatcct
    70081 tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc aatactgtca
    70141 gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg catgagatct
    70201 gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc gtagttttcc
    70261 atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac tcgttttacc
    70321 gaacgataag ttcttcctcc taaaaacaac accgcaccaa taaccgtcag aatgatcatg
    70381 taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc tagttatttt
    70441 gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc ttgatttctg
    70501 tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg aaaatgatcg
    70561 ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact ttacacagta
    70621 ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag cgaaatttac
    70681 tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga cggcagcggc
    70741 cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta tctagtagat
    70801 agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca gttaatggcc
    70861 gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt tcttggattt
    70921 agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa agttcatcga
    70981 ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc
    71041 agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa
    71101 aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa
    71161 aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat
    71221 tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa agaacaagga
    71281 catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca atttctttct
    71341 gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca tacaatggct
    71401 gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg tcattattgt
    71461 cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag aaacgtttgc
    71521 cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct ctgtgttacc
    71581 tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta
    71641 cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt tcggcgggtt
    71701 tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca gaaacgtgat
    71761 ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt acagtctgct
    71821 ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat attggatatg
    71881 gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt tcaaaaagaa
    71941 aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag tttcattgtt
    72001 ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg aaatccggat
    72061 tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa tcgtgtctat
    72121 gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt ccgctattgg
    72181 tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct actacatcat
    72241 tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt ttgatcagcg
    72301 ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg tggttgtctt
    72361 attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag cttttacaga
    72421 gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc aatttcctag
    72481 tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac gtgtgaaacg
    72541 agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt tattaagtga
    72601 ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc ttgcttctaa
    72661 aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga tcgatggaat
    72721 cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc tgccggattt
    72781 ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa agatttggtt
    72841 ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg ttcatttcct
    72901 tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct
    72961 tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata agcggatgtt
    73021 tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct tcaataataa
    73081 atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag caatcgaagc
    73141 ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa caagcgaaga
    73201 gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg aagaagctta
    73261 tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc gtgctgtctt
    73321 gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat tttctactga
    73381 gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa aggaaaaagg
    73441 gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg cagttgtcaa
    73501 aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca cgaacaataa
    73561 agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga
    73621 tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat ggttcaattt
    73681 agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac aggaaaggat
    73741 tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat taagagaata
    73801 taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta gagatagttg
    73861 gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg agcagacaca
    73921 aggtagaata agtggtttga aaaaatggat acgaagaaag tattaaaaac tgcataccga
    73981 tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt tcatgcttct
    74041 acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat cctgtttgga
    74101 ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct
    74161 tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat tgagaatgtc
    74221 gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac gttgtcacca
    74281 ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg
    74341 aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc ttcttctttt
    74401 gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc tggtttagca
    74461 agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac gcctacgtta
    74521 tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt tacaactgct
    74581 ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg aacagcacca
    74641 cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac gtcttccact
    74701 ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc atctactgta
    74761 tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc ttttaatgcg
    74821 gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa taactcgcga
    74881 acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac tttgttcaag
    74941 aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc acgtgtttga
    75001 ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg tgctgcccct
    75061 gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt
    75121 ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct
    75181 ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt gcctaatact
    75241 gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg
    75301 ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa acctcctaaa
    75361 taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt tttttgacta
    75421 aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct tattatacgt
    75481 caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa aaagtacctt
    75541 ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta actttctttt
    75601 gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa caactttcaa
    75661 tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat gaaaaaatta
    75721 attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc ttatcattat
    75781 ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat gtcagctacc
    75841 caagcagata acggagagaa attcacgact tatacctaca agcaagcggc ttataataaa
    75901 gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt aaagatgaat
    75961 gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga agaaataaag
    76021 gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa
    76081 ttttattaga taaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt
    76141 tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt
    76201 tgtctcacgc tctttttttg ttatattcgt cttttccgat ccttctcttg acacaattca
    76261 tttaactgat cactggtact tgaacaccta atattctagt catttttata aacgattcaa
    76321 tacttcatcc acatttttga tcatcacgga tatcgtacca tccggattat tcacaaattc
    76381 aatcaaatct gggttgcgat atacatctac tggtacgatc agttcgatcc cgtttgatag
    76441 cttgaatttt tgttttccaa attttttctc agaaatttct tgtacttctt ttacaggagg
    76501 tgcttctggg acaaatcctt gctcttgtac ttcttcttta aaagccattt ttgccgtgat
    76561 gttttccttg aagacttttt ccgcaatcat ctcatgatcc aaccgacctt tttcttctat
    76621 cgtatcatag acagcttctt tcaaatcagc catgatatca aattcttctg tctcaaattt
    76681 ttttccaaga tgcttggcta ctttttggat cacttttaca ttttcttcta aagaaggtgc
    76741 aggaacactt tcgatcactc ttccagaaaa ataaagctct tttttgccag aaaactcata
    76801 ttttttttca attaattcgt aggtccaatc agtcaaattc acagcgaatg cttcatctgc
    76861 tttttgtgtt ttcgccccta gaattgctcg attcaaaatc aagcggttgt cgatccctgc
    76921 ttcgtctgct tcaacaaagt gagtgtaagc ctcacgataa ttgactttca ataaagataa
    76981 gtacatcact gtatctaatt catacaagac aacaaataca tctgcactag gtgcttcttc
    77041 actttcttgg tagatatcga accaccgctg aacgattgct tcacttgctt ccacaaaatt
    77101 ttcttgagcg attttaagtg attcaacgat catgctttct tctgctagtt gtccagtttt
    77161 tgtttgagca gaagagattt tttggatctt cttttgaaga aaatcacgaa tatattcttt
    77221 tgtcagatcc agctcttttt gtgaaaagac tggagaacca gtttctctgt caataatatg
    77281 cagaattgct ttttttaaat agatatccat tttctcattc ccttctgtct tagtttacgg
    77341 aaaaaaaatc aaaaatacca gaatcaaaat cattttcttt taaacaaaaa tttcaagagt
    77401 agaaacaaaa tggattcttt ttttcgaaaa atgcgctaca cttatagttg aacgaataaa
    77461 aaaggagctg aaagtatgaa ttcattaaca agcacttatc gtctatcaaa tggttacgaa
    77521 atcccggtag taggattcgg cacatggcaa acacctgatg gggatgtcgc tgtttcctct
    77581 gttaaagaag cgctggctgc aggttatcgc catatcgata ctgcccaagg atacaagaat
    77641 gaagaaagcg tcggccaagc gattaaggaa agtggaattc ctagagaaga gatattcttg
    77701 acaacaaaat tatggaatgc aaaccacagc tatgaacttg tgatgtcttc ttttgaagaa
    77761 tcattgaaga agctccaaac agattattta gatctgtttt tgatccactg gccaaaccct
    77821 gttgcttttc gtgataattg ggaacaagca aatgcagata catggcgagc atttgaagaa
    77881 ctgtatgaag caggtaaaat caaagcaatc ggtgtgagca atttcttacc ccaccatcta
    77941 gatacactag caaaaacagc aaaaattatg ccgatggtca atcaagtttt ccttgcacca
    78001 ggcgaattac agacaactgt agttgagtat gcaaaaaaac atgatatgat cctcgaagca
    78061 tatagtccat taggcactgg taaaatcttc gatgtgcctg aaatgaaaca aatcgctgaa
    78121 gcacatgaca agacgattgc acaagttgct ttgcgttggt cgctgcaaca cgaattcctt
    78181 cctcttccta agtcagtgac accaagcaga atcaaagaaa atactgaatt atttgatttt
    78241 gaattaacag aagaagaaat gaaacaaatc gatcagttag atggtgtagt cggcaaagca
    78301 aaagacccag atactaccca attttaagtc gaaaggtgat tttatggatc caatcgaacg
    78361 cttgaattct ctctcggaag aagttaccca gacttttcat tccgattttg tctttttgat
    78421 cgatgcagag aaaatccaac attttcctgc aagaaactgg acacatgatc aaatcatcga
    78481 agaactgaaa aaacggttcg atcattcgct aatggtcaaa ccctggcacg aacatgaagt
    78541 catttattca cctgaactac ctgtttttgc cttgataccg aaaaaataga aagaatggaa
    78601 cggattatgt atatatacat aatccgttcc attcagactg tagacaaaaa ggagtggctt
    78661 aattgccaac tcctttttgt tagtctttgt agaaatttta aataaaaagc cataggcttt
    78721 tttgctttcc atagccatgt ggctagtttt tttaaattca tggcagcaaa cgtaagcatc
    78781 gtgtgcgtgg cgactttttc gagacctcga tatttcgtcc aacgcatacc atgcttttct
    78841 tttgcatctg caaatattcg ttcaatggtt tgttttctct ttttataaat ttctctattc
    78901 aacgccgtat ggcgttggtg ttcaagttca tccataagat ccgcccaaac atgtcgagtt
    78961 actatttttt gatgttttgt actcaaagta cagtttttaa gtaatggaca actcctacag
    79021 aattttggat ttgatttata ggttcgataa ccatcacgag tgatagttga aaataacaac
    79081 ggttgattat tgggacataa atagcagtca aaataggcat cataaacaaa gtctttttta
    79141 cgaaacattc cttttactcc atgaggtaca gtatatggaa gaatcgggta gagtttcaag
    79201 tgtgacagaa aatgaacaaa tttaggcgtc ttatatcctg catcagcgac aacacgcttt
    79261 atatttggaa aagctctttt cgactgtttg accagatcga ttgcgacttg gctgtcatgt
    79321 acatttcctg gtgtgattaa tgtagataag ataaaaccat tatcttcaca ggaagtatgc
    79381 gcggaataag caaattgttt ttctcgttca cctttaacat agtaaccact ttctggatct
    79441 gaatgactta ttttttgaag actcaactca ctttctgtgt cccatgtaaa aggacgtttt
    79501 cctacaacaa tacgttcttt atttatttca tcttctaatt catcttgata agctttagct
    79561 tgaccgtagg tcatttcttt tgtgaactta tgtttattgg catttgcctt aatatgagta
    79621 gaatcaatat aaaggttgtc ttccgtaacg aagccagctt ttacagcttg ttctagaata
    79681 tgagagaaaa tgtcttcaaa taaagttgtt tctctgaatc ttcgaacata gtttttacca
    79741 aaagtagaga aatgcgggat aggatcctcg aatgaataac ctaaaaacca acgataagca
    79801 acatttgttt caacgtcttt aatagtctgt ctcatcgaac ggataccaaa taaatattgg
    79861 ataaatacta atttaattaa gaccactgga tcaatactgg gacgaccatg ggtcgaatac
    79921 gtagattcta caatgggata aatgaaatca aactgaatcg cagcatcaat ctttcgaact
    79981 aagtgatctt taggtacaag atcatcaatt gttaacatag caaattgatt tctaccttca
    80041 atcgattgtt ttctcatcat tagaatcact cccagaatag tttatacaat gagtataaaa
    80101 caaaaaaact gcaaacaaca ctcccaattt agga
//
LOCUS       DAZKCN010000002        65888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000002 DAZKCN010000000
VERSION     DAZKCN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.002"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..2096
                     /locus_tag="ACG8K3_000075"
     CDS             330..2096
                     /locus_tag="ACG8K3_000075"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9143992.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2198..3457
                     /locus_tag="ACG8K3_000076"
     CDS             2198..3457
                     /locus_tag="ACG8K3_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9143993.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3604..4905
                     /locus_tag="ACG8K3_000077"
     CDS             3604..4905
                     /locus_tag="ACG8K3_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9143994.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4908..5759
                     /locus_tag="ACG8K3_000078"
     CDS             4908..5759
                     /locus_tag="ACG8K3_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9143995.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5772..6587
                     /locus_tag="ACG8K3_000079"
     CDS             5772..6587
                     /locus_tag="ACG8K3_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP9143996.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6738..7904
                     /locus_tag="ACG8K3_000080"
     CDS             6738..7904
                     /locus_tag="ACG8K3_000080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9143997.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8134..8781
                     /locus_tag="ACG8K3_000081"
     CDS             8134..8781
                     /locus_tag="ACG8K3_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9143998.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8865..9809
                     /locus_tag="ACG8K3_000082"
     CDS             8865..9809
                     /locus_tag="ACG8K3_000082"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP9143999.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9944..11395
                     /locus_tag="ACG8K3_000083"
     CDS             9944..11395
                     /locus_tag="ACG8K3_000083"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP9144000.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11416..12252
                     /locus_tag="ACG8K3_000084"
     CDS             11416..12252
                     /locus_tag="ACG8K3_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9144001.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12252..12911
                     /locus_tag="ACG8K3_000085"
     CDS             12252..12911
                     /locus_tag="ACG8K3_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9144002.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            13037..13726
                     /locus_tag="ACG8K3_000086"
     CDS             13037..13726
                     /locus_tag="ACG8K3_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144003.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13732..14688
                     /locus_tag="ACG8K3_000087"
     CDS             13732..14688
                     /locus_tag="ACG8K3_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP9144004.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14732..15244
                     /locus_tag="ACG8K3_000088"
     CDS             14732..15244
                     /locus_tag="ACG8K3_000088"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144005.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15500..16048)
                     /locus_tag="ACG8K3_000089"
     CDS             complement(15500..16048)
                     /locus_tag="ACG8K3_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9144006.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16137..17318)
                     /locus_tag="ACG8K3_000090"
     CDS             complement(16137..17318)
                     /locus_tag="ACG8K3_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP9144007.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17554..19062)
                     /locus_tag="ACG8K3_000091"
     CDS             complement(17554..19062)
                     /locus_tag="ACG8K3_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP9144008.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19221..19412
                     /locus_tag="ACG8K3_000092"
     CDS             19221..19412
                     /locus_tag="ACG8K3_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144009.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19615..20532
                     /locus_tag="ACG8K3_000093"
     CDS             19615..20532
                     /locus_tag="ACG8K3_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP9144010.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20525..21598
                     /locus_tag="ACG8K3_000094"
     CDS             20525..21598
                     /locus_tag="ACG8K3_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144011.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21746..22264
                     /locus_tag="ACG8K3_000095"
     CDS             21746..22264
                     /locus_tag="ACG8K3_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144012.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22245..22637
                     /locus_tag="ACG8K3_000096"
     CDS             22245..22637
                     /locus_tag="ACG8K3_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP9144013.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22713..23648
                     /locus_tag="ACG8K3_000097"
     CDS             22713..23648
                     /locus_tag="ACG8K3_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9144014.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23781..24560
                     /locus_tag="ACG8K3_000098"
     CDS             23781..24560
                     /locus_tag="ACG8K3_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9144015.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24553..24870
                     /locus_tag="ACG8K3_000099"
     CDS             24553..24870
                     /locus_tag="ACG8K3_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9144016.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25114..26724
                     /locus_tag="ACG8K3_000100"
     CDS             25114..26724
                     /locus_tag="ACG8K3_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9144017.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26881..27729
                     /locus_tag="ACG8K3_000101"
     CDS             26881..27729
                     /locus_tag="ACG8K3_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9144018.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27960..28439
                     /locus_tag="ACG8K3_000102"
     CDS             27960..28439
                     /locus_tag="ACG8K3_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP9144019.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28472..30700
                     /locus_tag="ACG8K3_000103"
     CDS             28472..30700
                     /locus_tag="ACG8K3_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9144020.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30829..32472
                     /locus_tag="ACG8K3_000104"
     CDS             30829..32472
                     /locus_tag="ACG8K3_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9144021.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32545..33090)
                     /locus_tag="ACG8K3_000105"
     CDS             complement(32545..33090)
                     /locus_tag="ACG8K3_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP9144022.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33113..33598)
                     /locus_tag="ACG8K3_000106"
     CDS             complement(33113..33598)
                     /locus_tag="ACG8K3_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9144023.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33588..34400)
                     /locus_tag="ACG8K3_000107"
     CDS             complement(33588..34400)
                     /locus_tag="ACG8K3_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP9144024.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34539..36071)
                     /locus_tag="ACG8K3_000108"
     CDS             complement(34539..36071)
                     /locus_tag="ACG8K3_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP9144025.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(36180..37211)
                     /locus_tag="ACG8K3_000109"
     CDS             complement(36180..37211)
                     /locus_tag="ACG8K3_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9144026.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            37935..38921
                     /locus_tag="ACG8K3_000110"
     CDS             37935..38921
                     /locus_tag="ACG8K3_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP9144027.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            complement(39070..40410)
                     /locus_tag="ACG8K3_000111"
     CDS             complement(39070..40410)
                     /locus_tag="ACG8K3_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9144028.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(40415..41089)
                     /locus_tag="ACG8K3_000112"
     CDS             complement(40415..41089)
                     /locus_tag="ACG8K3_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9144029.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            41311..42384
                     /locus_tag="ACG8K3_000113"
     CDS             41311..42384
                     /locus_tag="ACG8K3_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144030.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            42390..43076
                     /locus_tag="ACG8K3_000114"
     CDS             42390..43076
                     /locus_tag="ACG8K3_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144031.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            43084..43731
                     /locus_tag="ACG8K3_000115"
     CDS             43084..43731
                     /locus_tag="ACG8K3_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9144032.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(43804..45195)
                     /locus_tag="ACG8K3_000116"
     CDS             complement(43804..45195)
                     /locus_tag="ACG8K3_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144033.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            45452..48841
                     /locus_tag="ACG8K3_000117"
     CDS             45452..48841
                     /locus_tag="ACG8K3_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9144034.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            48844..50265
                     /locus_tag="ACG8K3_000118"
     CDS             48844..50265
                     /locus_tag="ACG8K3_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9144035.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            50262..52139
                     /locus_tag="ACG8K3_000119"
     CDS             50262..52139
                     /locus_tag="ACG8K3_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9144036.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            52231..53073
                     /locus_tag="ACG8K3_000120"
     CDS             52231..53073
                     /locus_tag="ACG8K3_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9144037.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(53225..54100)
                     /locus_tag="ACG8K3_000121"
     CDS             complement(53225..54100)
                     /locus_tag="ACG8K3_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9144038.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            54307..55338
                     /locus_tag="ACG8K3_000122"
     CDS             54307..55338
                     /locus_tag="ACG8K3_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9144039.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            55427..55732
                     /locus_tag="ACG8K3_000123"
     CDS             55427..55732
                     /locus_tag="ACG8K3_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP9144040.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            55911..57926
                     /locus_tag="ACG8K3_000124"
     CDS             55911..57926
                     /locus_tag="ACG8K3_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9144041.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIKKGDE"
     gene            57928..58212
                     /locus_tag="ACG8K3_000125"
     CDS             57928..58212
                     /locus_tag="ACG8K3_000125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9144042.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            58238..59611
                     /locus_tag="ACG8K3_000126"
     CDS             58238..59611
                     /locus_tag="ACG8K3_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9144043.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(59694..61709)
                     /locus_tag="ACG8K3_000127"
     CDS             complement(59694..61709)
                     /locus_tag="ACG8K3_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFP9144044.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(61731..62945)
                     /locus_tag="ACG8K3_000128"
     CDS             complement(61731..62945)
                     /locus_tag="ACG8K3_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9144045.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            63166..64110
                     /locus_tag="ACG8K3_000129"
     CDS             63166..64110
                     /locus_tag="ACG8K3_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9144046.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            64406..65350
                     /locus_tag="ACG8K3_000130"
     CDS             64406..65350
                     /locus_tag="ACG8K3_000130"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9144047.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT        21856 a        11832 c        12766 g        19434 t
ORIGIN      
        1 ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt
       61 ctagaatata cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa
      121 attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt
      181 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
      241 tgttaccgat aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga
      301 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
      361 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
      421 aaaatgatat caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa
      481 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
      541 gggaaattga agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg
      601 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
      661 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
      721 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
      781 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
      841 aagacttttt cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc
      901 ttggcatcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
      961 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
     1021 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
     1081 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
     1141 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
     1201 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
     1261 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
     1321 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
     1381 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
     1441 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
     1501 ttacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
     1561 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
     1621 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
     1681 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
     1741 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
     1801 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
     1861 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag
     1921 ataagctcta tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt
     1981 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
     2041 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
     2101 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
     2161 gtgtaacagc acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
     2221 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
     2281 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
     2341 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
     2401 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
     2461 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
     2521 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
     2581 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
     2641 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
     2701 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
     2761 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
     2821 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
     2881 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
     2941 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
     3001 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
     3061 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
     3121 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
     3181 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
     3241 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
     3301 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
     3361 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
     3421 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
     3481 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
     3541 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
     3601 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
     3661 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
     3721 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
     3781 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
     3841 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
     3901 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
     3961 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
     4021 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
     4081 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
     4141 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
     4201 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
     4261 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
     4321 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
     4381 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
     4441 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
     4501 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
     4561 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
     4621 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
     4681 aataatttct ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga
     4741 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
     4801 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
     4861 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
     4921 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
     4981 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
     5041 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
     5101 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
     5161 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
     5221 gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta
     5281 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
     5341 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
     5401 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
     5461 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
     5521 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
     5581 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
     5641 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
     5701 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
     5761 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
     5821 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
     5881 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
     5941 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
     6001 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
     6061 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
     6121 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
     6181 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
     6241 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
     6301 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
     6361 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
     6421 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
     6481 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
     6541 tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga
     6601 tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga
     6661 aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt
     6721 ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt
     6781 tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg
     6841 acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa
     6901 gtaacgcttc tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa
     6961 gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag
     7021 attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata
     7081 tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga
     7141 cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata
     7201 aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg
     7261 gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg
     7321 ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt
     7381 tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg
     7441 atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt
     7501 tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc
     7561 gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg
     7621 tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt
     7681 tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc
     7741 aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat
     7801 atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc
     7861 ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact
     7921 gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg
     7981 tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa
     8041 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
     8101 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
     8161 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
     8221 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
     8281 agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc
     8341 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
     8401 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
     8461 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
     8521 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
     8581 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
     8641 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
     8701 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
     8761 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
     8821 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
     8881 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
     8941 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
     9001 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
     9061 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
     9121 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
     9181 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
     9241 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
     9301 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
     9361 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
     9421 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
     9481 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
     9541 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
     9601 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
     9661 gcgcaagcat gttacagatt ggtacagagc ttcataaaga agggccggaa attttttcta
     9721 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
     9781 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
     9841 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
     9901 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
     9961 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
    10021 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
    10081 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
    10141 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
    10201 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
    10261 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
    10321 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
    10381 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
    10441 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
    10501 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
    10561 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
    10621 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
    10681 acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc
    10741 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
    10801 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
    10861 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
    10921 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
    10981 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
    11041 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
    11101 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
    11161 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
    11221 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
    11281 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
    11341 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
    11401 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
    11461 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
    11521 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
    11581 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
    11641 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
    11701 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
    11761 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
    11821 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
    11881 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
    11941 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
    12001 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
    12061 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
    12121 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
    12181 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
    12241 gtgagtgagt gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt
    12301 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
    12361 atttggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
    12421 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
    12481 tatatttcgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
    12541 ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag
    12601 gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg
    12661 ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct atggatctag
    12721 tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga
    12781 tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt
    12841 gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatccatc
    12901 agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt
    12961 cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg
    13021 ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca
    13081 atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg
    13141 aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta
    13201 tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca
    13261 ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac
    13321 aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg
    13381 tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt
    13441 gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg
    13501 aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa
    13561 tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca
    13621 ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag
    13681 gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat
    13741 ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga
    13801 ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa
    13861 aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa
    13921 gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc
    13981 tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct
    14041 ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag
    14101 ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg
    14161 cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat
    14221 gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat
    14281 attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc
    14341 cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat
    14401 tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga
    14461 gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg
    14521 attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag
    14581 tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg
    14641 cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta
    14701 aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga
    14761 agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
    14821 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
    14881 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
    14941 aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
    15001 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
    15061 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
    15121 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
    15181 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
    15241 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
    15301 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
    15361 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
    15421 aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg gcttatccta
    15481 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
    15541 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
    15601 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
    15661 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
    15721 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
    15781 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
    15841 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
    15901 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
    15961 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
    16021 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
    16081 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtattttat
    16141 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
    16201 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
    16261 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
    16321 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
    16381 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
    16441 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
    16501 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
    16561 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
    16621 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
    16681 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
    16741 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
    16801 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
    16861 atggaaataa aaacttccga ttgccctaaa atagcagaag ctactccatt ataagattcc
    16921 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
    16981 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
    17041 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
    17101 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
    17161 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
    17221 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
    17281 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
    17341 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact
    17401 tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt
    17461 taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata
    17521 gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg
    17581 gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt
    17641 attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc
    17701 aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat
    17761 gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag
    17821 aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt
    17881 taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc
    17941 atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat
    18001 cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac
    18061 ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa
    18121 gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac
    18181 ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata
    18241 aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac
    18301 atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat
    18361 attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa
    18421 cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg
    18481 gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg ggccaggagc
    18541 tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc
    18601 tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt
    18661 gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg
    18721 agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa
    18781 gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc
    18841 agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt
    18901 cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctaacttccc
    18961 accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg
    19021 ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt
    19081 actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa
    19141 aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt
    19201 tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact
    19261 atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat
    19321 tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc
    19381 atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga
    19441 ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt
    19501 gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg
    19561 tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa
    19621 gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa cgcggatcag
    19681 gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt
    19741 tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct
    19801 gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa
    19861 aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt
    19921 ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact
    19981 tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg
    20041 cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa
    20101 aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat
    20161 gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg
    20221 aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct
    20281 aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa
    20341 gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa
    20401 caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa
    20461 ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc
    20521 gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca
    20581 tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga
    20641 aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc cgacgaatac
    20701 gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg
    20761 aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc
    20821 ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata
    20881 tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga
    20941 atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc
    21001 agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa
    21061 cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat
    21121 gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct
    21181 ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt
    21241 caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca
    21301 gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac
    21361 gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga
    21421 aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac
    21481 aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt
    21541 cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt
    21601 ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt
    21661 tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc
    21721 tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt
    21781 gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat
    21841 gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat
    21901 gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca
    21961 atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt
    22021 gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat
    22081 aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct
    22141 tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt
    22201 tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt
    22261 atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata
    22321 cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt
    22381 tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa ggcgatgatt
    22441 tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc
    22501 ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga
    22561 aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca
    22621 atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca
    22681 gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt
    22741 tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct
    22801 atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca
    22861 gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc
    22921 atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt
    22981 tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc
    23041 atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag
    23101 gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca
    23161 cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact
    23221 tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg
    23281 ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg
    23341 ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg
    23401 atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa
    23461 aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta
    23521 acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc
    23581 ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca aattattctc
    23641 gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt
    23701 aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg
    23761 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
    23821 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
    23881 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
    23941 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
    24001 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
    24061 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
    24121 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
    24181 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
    24241 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
    24301 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
    24361 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
    24421 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
    24481 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
    24541 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
    24601 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
    24661 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
    24721 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
    24781 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
    24841 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
    24901 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
    24961 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
    25021 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
    25081 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
    25141 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
    25201 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
    25261 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
    25321 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
    25381 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
    25441 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
    25501 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
    25561 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
    25621 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
    25681 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
    25741 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
    25801 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
    25861 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
    25921 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
    25981 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
    26041 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
    26101 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
    26161 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
    26221 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
    26281 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
    26341 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
    26401 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
    26461 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
    26521 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
    26581 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
    26641 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
    26701 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
    26761 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
    26821 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
    26881 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
    26941 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
    27001 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
    27061 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
    27121 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
    27181 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
    27241 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
    27301 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctacgc ttatctgatt
    27361 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
    27421 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
    27481 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
    27541 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
    27601 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
    27661 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
    27721 gcgcaataat tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact
    27781 ttctacatgt tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg
    27841 aagctctttt tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta
    27901 aatgagtaaa ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa
    27961 tggaaatacc aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag
    28021 ttgatggaag aaatgcatta aatacggatg caaaagggct ttcactgggc cttgctcagt
    28081 ggaatgatcg tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat
    28141 ggtcacgaca gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg
    28201 cacaaggaag tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa
    28261 atccagtcgt agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca
    28321 aaaatccaaa gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag
    28381 aactgattgg ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat
    28441 agacagagat gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg
    28501 aaccttttca cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag
    28561 tgcagaagaa tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa
    28621 aatcagtaac gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc
    28681 actatctgct gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa
    28741 atcagatgtt ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga
    28801 attactagaa gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa
    28861 ccattacggc attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat
    28921 cgaagctgat tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc
    28981 gattgaaaaa ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt
    29041 tcttgtggca cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat
    29101 cattcgtcgc catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa
    29161 tcgcccatta aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc
    29221 tgccttagaa gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact
    29281 tctcttaaag atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat
    29341 caaaggtgta gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca
    29401 agatttactc cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga
    29461 attggcaatt cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat
    29521 attgagaaaa gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag
    29581 cgccgaagaa gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga
    29641 tgaaaagaaa tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac
    29701 aatagaaaaa aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg
    29761 aaaaaatcaa aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat
    29821 ggaagcacat gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa
    29881 ggaaacgatt catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac
    29941 gccggaagca cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca
    30001 aattttagag ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg
    30061 tcagcggttc aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa
    30121 cccgccacat gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat
    30181 gatacgggat gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc
    30241 taagaaagtc gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga
    30301 aaagacttct ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa
    30361 attagtcact atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc
    30421 agaaaaatta aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca
    30481 gccaaaagcg ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt
    30541 gaaagaatat gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga
    30601 agaaatcaag tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt
    30661 tcccaagcaa atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac
    30721 gcacttagca gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa
    30781 aatagaatcg gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa
    30841 caaaaatggt ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc
    30901 aatggcgatg gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat
    30961 cttggtatcg gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt
    31021 tatgacgtaa gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat
    31081 ttattgctaa aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac
    31141 catagttcgg atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat
    31201 cgcgattatt acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg
    31261 attttcggcg gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt
    31321 tttgcaaaag aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc
    31381 aatatgatcc gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct
    31441 catgtaaaaa aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa
    31501 aatgtctcag gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat
    31561 atcatgactg tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg
    31621 aaagacggtt atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa
    31681 aaagaagggc aattagatgt ccttaaattg aaacttgcat taagcgcttg gcaaacttca
    31741 ttggatggta tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt
    31801 tcagtgtttg gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg
    31861 atgtatttct ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg
    31921 aatatgccct ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa
    31981 aggctgcttg cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg
    32041 agagacaaca gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca
    32101 catgaacctt ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa
    32161 tatgaaccag agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat
    32221 aaaggtctga tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc
    32281 tatgaaagat atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt
    32341 gctgactatg aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc
    32401 tctagcggag aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa
    32461 acaaacaagt aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc
    32521 agattgctct gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg
    32581 acgagagcga agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg
    32641 tacttggaca tgtttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg
    32701 tccaactacg tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca
    32761 agcacctcca gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc
    32821 cactgttcgt tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc
    32881 aatttcttta ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc
    32941 actggaattt tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa
    33001 aagatcacct tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg
    33061 gtcttttaat tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt
    33121 gcttccgtgc ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa
    33181 tcagattttg gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa
    33241 tccaaccggc gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc
    33301 cccacatacg agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga
    33361 tcactaatga aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac
    33421 tggaagcacc aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc
    33481 cggcctcgca taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg
    33541 aaagggcaac tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt
    33601 ctcattcctc ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc
    33661 acgccattag aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa
    33721 tctgccccta acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt
    33781 actgttgtag tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga
    33841 agatccacag cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt
    33901 cctgaagcag tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct
    33961 tgaccagaaa gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa
    34021 agttcttcga tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat
    34081 gccaaaacag ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca
    34141 accatgattg cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt
    34201 ttatcaatcg aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat
    34261 cgctcattaa tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa
    34321 ttctcaaaag tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca
    34381 attgtcaaga ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca
    34441 aaaaagaaaa acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga
    34501 aaagaaagac caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat
    34561 tctttctcga tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa
    34621 tcacggatga tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc
    34681 caatccattt ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc
    34741 cttacaatca ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct
    34801 aatgtactat tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca
    34861 tgcttatcca taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg
    34921 aaccgtgcat cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga
    34981 ccatctgtgt tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg
    35041 tcttccagtc caacgcccat atctcttgca tcacttgttt tcaaactctc tagtgtatga
    35101 gccttaagaa tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc
    35161 tgtgccatcg acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg
    35221 gagtgatagt atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac
    35281 tgaacaaagt tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga
    35341 acaacttttg aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa
    35401 gaagagatac caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc
    35461 ttttccgaaa tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga
    35521 gcagaatgac aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa
    35581 atttcatttt tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa
    35641 agaataggaa tctgttttga ttttgttcca tttgaaattt ctctcactcg cttttttgtc
    35701 tcatcatcaa ataaaatgaa agggtcttct aaacgatcga taaatacttc cattgttgtt
    35761 gttggtagat gataagaagt catgacaggc actgcaccaa ggtaagacac agaaacagct
    35821 agaaggtatg tgtcaaaagc ggaactctta taaataataa ctttgtctcc ggatttcact
    35881 ccaagatgag ccaattgata agcccgtttc aacacacttt cgcagcattt tttgtaagtt
    35941 gtttcaagtc ctaattctgg gaaagctgaa agtggctcat caaagataat cggggtctcg
    36001 gaaaagttag ctgcagcttc ttgaaaattc gtaaataagt tcaaagggga atacgttatg
    36061 gtctccatca ttcacactcc attatctttt gttaagtaat atacaaattc aagataatcg
    36121 taacataatc ctttatataa taacatagtt tattgtgaaa aaaagatcat acatttgttt
    36181 tatatggaag aaacccgaac gaatgccgct aattcgtgat aagaaaaatc aaccatcttt
    36241 tcccagatat cttgaaaaga atgggatgag aggtatcgat taccacataa ataatttacc
    36301 gcgtcagcat catctaaata tagttctgca taataacgat atgtttttct gcatagttct
    36361 ggcgatacgt ttttatatac tgtaattttg ctcaaaattt ggcgaatagt ccgaggagaa
    36421 atttgctgat catgtgagga acgaaaaaca aaagaatttt cgtccgtatt ttgtgctgtt
    36481 aacttaaaat agttctccag aaatggcatc gcttcttttg gtattggtac tttattcagt
    36541 ctgttttctt ttctattcag attaagaatc tgcttggtca aagatacatc tttacgttta
    36601 agttctacta cttcatggat ctttaatccg caatacagaa gaatggcagt aattgcaagg
    36661 ttcctcggtc tattcttttc ttcaaagcta gcttctctgc ttgtttgaaa gccttcaatc
    36721 gggataaaca aatgctcaaa atctttgaaa agttctgcat cgatatatcg cggagctgct
    36781 ttggttgttt ccggtaaact tgtacccaaa agagaaatct cttcttcaat cagtcctttt
    36841 tgatacaaaa atcgtaaaaa atgatttaat gaatattgct tccttgaaat cccgtaatag
    36901 ctattttcaa acaattgctc cgtttcttgg ttcgctgtag tttggaacga acgagtatgt
    36961 cgatagattt tagctaagaa attctgataa tcttgttttt ctaaatggat aatcagctga
    37021 tcatccactt cttcagaaaa tacttttctc aaataatcag taaacagatg aaaatctttt
    37081 gcgtattcat agcgactgcc taatgaaagg gaagaacgat tttcttctaa aaactgttga
    37141 aagacggaag ggagcaagtg tgtacgttgc tttaactttt cttgcagttg ttttctagtt
    37201 ttgctgtcca ttacaaattc cctccaatag tgttcatttt ctatatttta ccgcatttca
    37261 tcaaatgaag atagacagtt tgtcttgatc ttaggaagca aatttttaac taagtactag
    37321 ctaaaagata tgaagtaaga agatacattt ttagttattt tattagtgca gtcattcttt
    37381 tgatctttta attgatttat cctcttttta gctatgtggt tacttcatga gcaatgaaat
    37441 ggatttggtt cgttctaatg gcaggaggct ctttctttta tttccgaaaa attagaaaac
    37501 attttttcag aatcgattgg ctctccattt attcaataat ggtttgtcta acgatattgt
    37561 tgggataatt cttaaagaag tattagaaat agttggtact tcattacttt acatgaagat
    37621 ttattctatg gtaggagcaa ttatattgag aattaatcct tttttatata agttgtttgc
    37681 ttattgatgg attggataaa ttaaaccatc acaacgagtg gaaattatat gaaatttttc
    37741 ataaaaattc atatagaaac aaatgacatt ttattagaat atgactttca ttaacataaa
    37801 tgttttcaaa tttcaattaa attgttttaa tcaaattcca atctaaatat ttgtagaaaa
    37861 caatcgtctc ctatagaata aatttataag taacaatata ccaatcataa atctatttgc
    37921 taggaggaat ttacatggaa gagaaaaatt taacggtcat cggaagctat agtacgaggg
    37981 aagaagcatt atcagtaatt gaacggttgc gtaacgaagg gtatgaacga gatgatatcg
    38041 ttatttatac tactgatgaa gcagcatcta gactaggatt tgacggattg tctggtatag
    38101 atgttgaaac agatgaaaat cggatggatg gtgaagaaga ccgttcttta tgggaaaaaa
    38161 taaaagatac tttttctttt gatacctatg attctgagac ggctacacca gaaaatgatc
    38221 ctttatatca atatagagga gatatttctg atggaaaatt tgtcatcaca gtaaaaggct
    38281 atcgtcagcc agaaacaaca gaagatacaa tggatacaca agatactgtt agcccagcaa
    38341 gcacaatggg gactggatta gaaactggca ctggaacaga tccaatggac gtaggtggca
    38401 cgacaggctt tcaaaataca acggatccat tgaatatgac accaggaact gatccgatgg
    38461 acgtacctgg cgagactgat acaatgggta caagcaagac tgaagacatc gatcgcaaac
    38521 caaatctaga tgatgataca attcaactaa aagaagaaaa attagatgta aatacacatg
    38581 atgtgacaac tggagaagtc gacatccaca aacatgtggt caacgatact gaaacggtag
    38641 aagtaccggt caaacgtgaa gaaatcgtca tcgaacgcaa accagtaaca gatcaatctt
    38701 cacagggaac agacgaaaaa ttggaagatg acacgattac tatcccaatc aaagaagagc
    38761 aagtagatgt aagcaaacat acagttattc gtgaagaagt tgggattcat aaagaagaac
    38821 atgaagatgt tgaaaaagtg acagaagatg ttagccgaga agaattggat attgacacaa
    38881 gcggtgatgt acacattgaa gaccgcaata aaaaatcgta agcgtaagct aaagaggttg
    38941 tgacagaagt ggtcagctta aagaaatttc ggcttatttc cgaagaagct acttctggaa
    39001 caccgtttat tcgcaacaaa aaagggctgt gacaagattt ttgtcacagc ccttttttag
    39061 ggataagcat tattttttag gcttcaacgg caattggact ttaaaatcga ctccttgtgg
    39121 aagataattg gatacagaaa tctgtccatg atgctgttcg attacctgtt ttgcaattgc
    39181 caagccaagc ccgtatccgc cagtctcttt cgcccgagat tgatcttccc gataaaatcg
    39241 atcaaaaatc cttgcttgat cttcttcact gatagaaggg ccagtattct ttacgtgaat
    39301 cagccattct ttatggttaa cagatgaatc caaaatgatc ttatcttctg gattcgtata
    39361 ttttaatgca ttatccagca aaataaccaa aacttgatgg attttttttt catcaattgg
    39421 tacttctaat gttgcatcgt tatggagaat aaactctttt ccatctatga tggccatctc
    39481 ttgaaatggt ttaacaagtt gttcgatata ttcttgtgta ttgatcattt gctttgaaag
    39541 cagctgctga tcagaatcat tccgagcgat tgtcagcaaa tccgaagtca atccagtgag
    39601 gcgcctcgtc tctgacaatg cttgagcgat gctttcggat tcttcaataa tagtatgatt
    39661 aggcttggta aaaagatgtt ccaaactatt ttgaataatc gttaacggtg tgcgcaattc
    39721 atgagaagca ttctcaacaa attctttttg tcttctccaa gaaagaataa tagggcgcat
    39781 gcttagacta gacaagtaat aactgatacc aatagagaga atccaaaaaa caatcataca
    39841 caaaatcata atcgtgcgaa aagtcgctaa tgaattcttg atttgatttg tattttctaa
    39901 aatttggatg taggcaacgc catcttgatt cgtctccgct tttttcgtaa tcgaacggaa
    39961 agagagggca gatgagccac tgtcttcatt taattcaata ttttggatgg tattcagatt
    40021 tttcgtatcc atttttaatt ctttgatttg gttgaaacgc ccaccaatag ctgctttatt
    40081 caatatctca ccatttttag accacaagat cacttgagtg ttaaaacggt catttgatgg
    40141 ttccattaat ggctgttcca tgtttgttct gttttgttcc gtttgagtcg ggtttaaaaa
    40201 gggatcgcct tgctgatatc tttgtatctc taattgtact gttcgagaat caaaatttat
    40261 tttctctaat gacagatctg tttctctgta tgcagattgg tttaatatct gtaaagtaat
    40321 gaatcccaaa agaaaaaaga taaaagcaaa tgctgctaca tttgtcgcaa aaaagcgact
    40381 acgttgcttt cgtgtaatat ctccattcat tgcatcacac cctttgcagt atataaccga
    40441 cattacgtaa ggttttgatt tctttatcca atccagtact tcgcagatgt tttcgtaagt
    40501 tgctcatata aacttcaata actgtaatcg tcgtatcaga atcaaatccc cagatccggt
    40561 caaatatctg ctcttttgtc aaaatgattc ccttattttg taaaaaataa acgagcaatt
    40621 caaactcttt tccttgtatg ggcaaaactt gttctttata caagacttca ttgcttttta
    40681 aatcacaact gatatcctta tacttcagaa cattttcatc aaagagacct agcgagcgtt
    40741 tcaataatgc tttcacgcgc atgatcagtt cttctcgata gaaaggtttt gttagataat
    40801 catctgcacc tcgttcaaat ccttcaatct tatcttccaa actatctttt gcagtcaaaa
    40861 tcaatactgg agtctgtatt ttttctttcc gtatttttgc taacacttca tatccattca
    40921 tttccggcat catcaaatcc aagattgcaa gatcataaac tccactttcc aactcgaaca
    40981 gtccttcact accatcataa acttgtgtta tctcgccgat tgcagcgagt atttctttta
    41041 tattattgga taatacttta tcgtcttcta ctactaatag tttaatcatt ttcccacctc
    41101 attgatttgg tcactttagt ttatcatgga aagcataaat aaggcttaaa taaatttttt
    41161 atgactcatg ttaaggtttt atcgaaagat gtcttagaca atttattgat aagaaaaaaa
    41221 caacacaagt gacaaattgt cactttagag ttacactatt caacaagtga cattttgtca
    41281 ctatcaatct atcataggga ggaaaaacaa atgtttctag ctatcaaaga aattcgctat
    41341 tctaaattac gatatggctt gatcgtcggt attatgttct tgattgcgta tgtcgtattc
    41401 atgttgtcag gactagcgaa tggactttca gaagagttta aaaaagcaat cgatgattgg
    41461 gacgcacaag agattgtttt gtcggaggat gcaaatcaag tatttgcagc ttcacaatta
    41521 acaagaggag atctgaaata cattgaagga ggagagaaag caccaatcgg attgtacagc
    41581 ggagcaatca aaggaaaaaa caaagaaaat gttacagttt tcggcacaac gaaagaagca
    41641 tttttattac caaaactcac gaaagggcat gaatttaaaa caaagaatga aatcatcatc
    41701 tctcaaaatc tggctgattc aggctataaa attggagata cgatcaaaat tggcagttac
    41761 gacgatcctt tgacgattgt cggtatcttt cctgaaactt attacacagt ttctcccgtc
    41821 atctatgcgt ccctagatac gtggactgct ttgaagtatg gacaacagcc gtttgcttct
    41881 gaagaagaac agccgatcaa tgggatcgta ataaaggatt cagctaaaat ttcgaaagaa
    41941 aaagcatcga agggtctaca gttattaact attgggacat tcattgaaag tattcctggc
    42001 tattctgccc aaaatatgac cttaaatgcg atgatatact ttttgttctt agtggttgct
    42061 gccgtggtcg gtattttcat gtacgtgatg acacttcaga agactgctat ttttggagta
    42121 atgaaagcac aagggatcaa aaatttcttc atcgctaaat ctttagtagc acagtccttt
    42181 attgttgggg tggtaggcgt tctgcttgct cttttgtttg cttatttgac tagtctgatt
    42241 ttaccaagcg cgatgccttt tgctgttttt tggtcacaat ggttgctata tagtgggatt
    42301 ttgatcattg tcgcaatgct aggcggattg ttttcgatac gtacgataac caaagtagac
    42361 ccaattacag caataggagg ttaataaaaa tgaaaaaaat attaacgatg aaaaaagtta
    42421 ccaaaacatt tggttctggt catacaaagg tctcagcttt gaaagcaatt gatttttatg
    42481 taaaggaagg agaattcgtc agcatcattg gtccttctgg ttcaggtaaa agtactttcc
    42541 ttaccatttc aggaggattg caaacaccga cttccggaga aatagtgatc aacggtcact
    42601 cactaactga gcttccagaa aaaaaacgag cagatttacg ctttaaagaa ataggattta
    42661 ttctccaaag ctctaatttg attccttttt taacggtaaa ggaacaattt gaactagttg
    42721 accaagttac ttcaaaaaaa gaagattcaa caaaaatcga ttccttgctg caatctttag
    42781 atatccagtc acttaaaaat agttatccta aagacctttc tggtggagaa agacagagag
    42841 tggcaattgc tcgcgcttta tataatgagc caagcttgat cttagcagac gaaccaacag
    42901 ctagtttgga tacagatcat gcatatgaag tcgtcaaatt actagttaaa gaagctcatg
    42961 agaaacaaaa agcgactatt atggttactc atgatgtccg tatgattcaa tggagtgatc
    43021 gcgtttatcg aatggaagat gggtacttga cagaagaaga gaaaaagaag gaatgaatcc
    43081 ttcatgacaa tgaagggcta tccacgtata atcagagata aaggagagat gtggatgcca
    43141 aaagaaactt ttttcaattt atcgaacgca aagaaacaga gaatcgatga cctattactg
    43201 gagatatttt gctcacagca catcagccaa gtcaaagtct cagaaatcgt tgacaagatg
    43261 gggatgtcgc gcggtgcttt ttataaatat tttcaagatt tggaagatgc ttatttgtat
    43321 acgatcaaaa gcagttctca catagtccat caagatatct taaaattcat tagcgaaaac
    43381 aaacaacatt tctttttggg gatcgaaaaa tatttagagt ggtgctgcga cttagatcac
    43441 gaaagtatct attggcaaag gttatacttg ttaacccagc gaaatgatca gcagacaacc
    43501 aaacgtagag aactatcggc tgattctcca atgaacgaaa aatggctgga attattaaga
    43561 atcaatcatt tctccattca atcgtcggaa gaagcagtta gtttcctata cttttccatg
    43621 accctcgtaa tgaacacttt gacggatctc attgcaaata attggacaaa agaagagttg
    43681 ttaaaagact ttcgctataa atctcgttgg attgctgaag gaatacaata aaaagaaaca
    43741 gctatgtgta tttgtcctga aatttttatc agggtggttt atacatagct gttttttgat
    43801 tattcataac tcgaataagc ggatgagttc ttcttcattt gcgaatgaca cactatcatc
    43861 aatcaatata tgaggaacat gggcattcaa aatgggttga ttcgtaacga tcagatcata
    43921 ctcgagaaga ttgtatttcg acagctgatc ttcagtaata gcagttgtct cagagtgaat
    43981 tttatgcaca gcaagaatat cactgatata ttcttcaata tattgactat gtcgaagact
    44041 taagtcgctg ataatcagta tttttttatc agaagtataa tggtgaattt caggattacc
    44101 gataagcatc caaaagatat agctagataa ataataaatc agattctgat tcagtagagt
    44161 ggaataaaat tgaagttctg cacgaaatcc agcatataga cgtatctggc ggtgttgcat
    44221 ctgctgtgca aagtaagttg cacggatcgt caaatgagtc gtatcgtatg ggaaaatacg
    44281 ggagaaaaaa taaatttgcg ttaataattg aacaacatat tcttcgtttt tttctttgca
    44341 atcagcggat gaaaacgaac agtaggacat attttttcgt atctgctcac tgatttcatt
    44401 tttttctttc tccgaaaatt ctgaaaaaat cgtattgaca aaatagttca ctacttttat
    44461 atagctgctg tacttcaatt ttcctaagag atttttcaag attaagaagt cttttttatc
    44521 gatatttatc aactgtacat aagagtctcc agctttccat tcaaatggag ttccttggga
    44581 ttctctcatg agatgcacaa caaaatacat aataaagaaa gattgctcat atgggctttc
    44641 ttcctgaaac aaattcatct gacaattatt cttgaccata atttctttga tcttcttttt
    44701 ttcgattgga aatggcaacg tgcgtcttcc gtagtaactt aaaaagaaaa aggtaacaaa
    44761 gatacgcaac tggagctcac ttttaaggct agttaactga aaaccttggt caacgatcac
    44821 ttttaataaa tagctttcta aaatttgatt gcattgtttc aacttacgtg taaaagaaga
    44881 ccggctgatg gaaatttttt cacaataata ctcaatcgtt ttacccggat tgacaataat
    44941 ctcatttaat aaacgactgt ttgtagaact ttgcgacatt tccatcagaa catttaaata
    45001 agctggtaca ttcaaatgtt caagacgata ccccaatttt tgtgaagatt gaatcgttac
    45061 atccgttccc cagcggattt tgatctcttc gatatacctt acaaatgtag attctgaact
    45121 accaattcgt ttggcaagtt cttttttagt catccacttg tcagaaatgg acagaattcg
    45181 aatgattttt tccatctttc tatagtttca ttccaacaac ctgatcatat gtttttctcc
    45241 ctcaaccctt ttctttcaat gaaacgctca ttaatcgaag gaatcaactt cataaatgaa
    45301 accaattggt atgaaaattg gtgtgattcc ctttagcgcc cttcgtataa taattatcat
    45361 attaaaataa ataaaattat gtaacgcctg tttattttaa taattttcac tattcacgtc
    45421 aaaacagaaa aaaagaaaaa ggaggggaaa aatgacgacc acagggaaga aactgaaagt
    45481 tattttcatg ctgataatat tgagtttatc aaactttgtg ccattatctg caatagcaga
    45541 cactacagat gatccaacag ttttagaaac aatttcagct gaagtcattt cggatcagtc
    45601 tggaaaaaaa gcactgaaca tcaagctaaa tgcgaataac accagtgctg aaaagataga
    45661 aaaagaaatt ggtctagtcg aaaattactt aagtgatgtg gaaagaaaag aaggagatgg
    45721 ctatgcttat caggtaaata gcgggaaaat tacgttggaa atctcatcaa acactaaaca
    45781 aactatcgat ctgagttttc caatcgatcc agcactttac cacagccagg caaacaagct
    45841 gatcgtcgat aataaagaat atgacattat tgatgagaca gaaaataaga aagatacaga
    45901 tgtgtcagta ccaaagccag acgaaataga agaagaatca tcaaaagaaa acgaaaattc
    45961 tgtcagccca tttacattgc ctacattatc cttgccagct gtgagtgtgc catctaatca
    46021 aacgattcct acagaatata caacagatga tcagggcact tatcctaaag ccagttggca
    46081 acctacagga aatacaaatg ttcttgatca tcaaggcaat aaaaacggaa caaatcaatg
    46141 ggatggtata aattcttgga atggagatcc taatgatcgg acccattcgt atatcgaata
    46201 tggaggaacc ggtaatcaag cagactatgc gatacgaaag tatgcaaagg aaacaagtac
    46261 acccggattg tttgatgttt atttgaatgc tcgtggaaat gtacaaaaag atatcacgcc
    46321 tcttgatctc gtattggtcg tagactggtc aggaagtatg aacgacaata atcggatcgg
    46381 tgaagtaaag attggtgtcg atcgttttgt cgatacttta gcagatagcg gtatcacaga
    46441 caaaatcaat atgggatatg tcggctactc aagcgaagga tatagctaca gtaacggtgc
    46501 agtacagatg ggttcatttg attcagtgaa aaatcaagta aaatccatta caccttcacg
    46561 gacaaatggt ggtactttta cacaaaaagc actaagagat gcaggaagca tgctatccgt
    46621 tccaaatgga cataaaaaag tgatcgtttt gctgacggat ggtgtaccaa cattttccta
    46681 taaagtacag cgggtacacg cacaatcaag cagcaattat tacggaactc agttttctaa
    46741 tacgcaagat cggccgggaa atacttctct aatctcaaga atctatgatg cacctgacca
    46801 aaacaatcta tccagaagaa tcgacagtac gtttatcgca accatcggag aagcgatggc
    46861 actcaaagaa cgaggaatcg aaatacatgg tcttggcatc caacttcaaa gcgatccggc
    46921 agctggtctc tcaaaagcag aagtagagtc tcgtatgcga caaatggttt catcagatga
    46981 aaaaggcgat ctttactatg aatcagctga tcatgcaaca gatatctctg aatacctagc
    47041 caaaaaagct gtacagatct cagcaactgt aagcaatgga caaataaatg atccaatcgc
    47101 agaaccattc atttatcagc ctggtacact ttcagtcaag agtgtgggga caagtcctac
    47161 aacggtcact ccatctattt ccatagaagg aaataccatc aagagcaatc agatctattt
    47221 aggaaaagac caagaaatcc aaatccatta ccaagtgaga atccaaacag aaaatgagga
    47281 cttccatcca aatttctggt atcaaatgaa cggcaggaca actttccagc caaacattga
    47341 taccaatgaa ttagctgaat tcggtatacc atctgctaaa gctcccggag tcagtcttca
    47401 catcaaaaag ttatgggaag aatttgacaa caatctagct gatcgtccag atcaagttac
    47461 ttttgagatt caacgggaac atacgacaaa tgctgcagct tggaaaaacg gatatattcg
    47521 aatcattaaa ccagctaaag atacaacaaa tacgtgggaa cgtgcagaca ttgacaaatt
    47581 atctgcaaat agcggagaaa gttatcaaga gatattatca ctacctcaat acaataatca
    47641 aggtcaagca ttcagttacc aaacaatcaa agaattacct gtaccaggat acgattctca
    47701 acaaatagat gcaatgacat ggaaaaatac taaacaattc acaccgttaa acttgaaaat
    47761 aacgaaaaat tcctctacag gtgaaaagga tcttattggc gctgttttca aattaacagg
    47821 agattctatt gatactttac taacagatca tggcgacgga acctattctc ttccagaaaa
    47881 tgtcaaattg caaaaagaaa tgacctatac gctgacagaa acaaaagctc cagaagggca
    47941 tggattaagc aaaaagacta cttgggaaat caagatcgct tctgatggta cggtaaccat
    48001 tgatggaaaa acagtcacta cttccgatga tacgatccag ttgactattg aaaatccttt
    48061 tgttgaagtt cctgtagcag tacgtaagta tgcgatgcaa gggacggaca aagagataaa
    48121 tcttaaagga gcagcatttt ccctacagaa aaaagaagca aatggtactt atcagccaat
    48181 tgacagccaa acaacgaatg aaaaaggtct tgccagtttt gattcactca cacctggtaa
    48241 atatcgagtc gttgaaacag ctggtcctgc cggatatgat acttcgccgg gaaattatga
    48301 attccaaatc gataaatatg gaaaaatcat ttacacggga aaaaataccg agatgacaaa
    48361 taatgtatgg acgctcactc atcaaaatcg actaaaagcg tttgatctaa cggtacacaa
    48421 aaaagaagac aacggacaga cattaaaagg agcaaaattc agactgcagg gaccagaaat
    48481 ggacttagaa tcgccaaaag atggacaaga aacagatacc tttctattcg aaaatttaaa
    48541 acctggaact tatacgctga ccgaaacttt tacaccagaa ggataccaag gtctaaaaga
    48601 gccagttact atagttatac acgaagatgg gtcaattcaa gtggatggac aagatcatga
    48661 atctgttctg tcaccaggag ccaaaaacaa ccagatttct ttagacatca cgaatcaggc
    48721 aaaagtacca ttacctgaaa cgggaggaat tggccgttta ggaatctatc tagtagggat
    48781 gattggttgt gcgttttcta tttggtatct ttttttgaaa aaagaaagag ggggcagcta
    48841 aatatgaaaa aacttggttg gcttagtatg tgtctcttct tgttactatt taaaccagct
    48901 tttactcagg tagcaacaga aacagaaaca gaaatggttc agattacttt acacaaattg
    48961 cttttcccaa acgggcaact gccgaaaaat catccaaatg acggacaaga aaaagcttta
    49021 ttacaaacgt atcgaggatt aaatggtgtc acattccaag tttatgatgt cacagattct
    49081 ttttaccatc tacgggaaaa gggcaaaacg gtagaagaag cacaagcaga gatcgcaaaa
    49141 aacggtgcgt cttccggtat gtttaccgca gaagcaacaa ctacaactct taacaacgaa
    49201 gatggtatcg cttctttttc tctggccgct aaagatcaag aaaaaagaga taaagcgtat
    49261 cttttcattg aatccaaagt accagaagtc gtcaaagaaa aggcagagaa tatggtagtt
    49321 gttcttcctg tacatggaca aaacaatcaa aaactttcaa ctatccattt gtatcctaaa
    49381 aatgaagaaa acgactaccc tgatccacct tttgagaagg tattagaaga gcctagaaat
    49441 gattttacga ttggtgaaaa aatcacttat tccttgcata cgacaattcc tgtaaatatc
    49501 cttgactatc aaaagttcga attgtcagat agtgcggatg aagcattaac gtttttacct
    49561 aatagtttaa cgatttcatc gaatggagaa aagctgacag aaggctttgt catacacaag
    49621 aaacctcacg gatttgatgt tttattttcg atcccttcgt tggaaaaata tgctggaaaa
    49681 aaactgacca tttcttatca gatgcagcta agcagtacag cacaggcgaa caaggaaatc
    49741 aacaacaacg gaacactgga ttttggtttt ggtgtcagta caaagaaagt ctctgtatat
    49801 acagggagta agcaatttgt caaaatcgag acaaataaac cagataaacg attagctggc
    49861 gcagtattcc ttattaaaaa caaagcagga aattacctcc agcaaacagc caacggatac
    49921 aagtggacaa agaacgaatc agatgcgctt cacctgattt ccgataaaaa tggcgctttt
    49981 tcaatttccg ggttgaaaac aggaagttat cgattaaaag agatcgaagc accttctggt
    50041 tatattttaa gtgaaacaga aattccgttt accatttcaa cttttctttc tgaggataaa
    50101 gaggcggaca gtatattgaa agtagtcaat aaaaaagaaa atagccgtcc atttcttcca
    50161 aaaacaaacg aaacgaaaaa tacactttta ggcgttgttg gtatggtatt cgcaagcttt
    50221 gcaatctggt tgtttatcaa aaaaagaaca ggagtgaaaa aatgaaaaat cataaaaaaa
    50281 taaacgttat gttaggagtc cttttcctta ttttaccatt actcacaaac agcttcggcg
    50341 caaaaaaagt gtttgcagag gagacagcag ctcaagtcat ccttcataaa aagaaaatga
    50401 ctgatttacc cgatccttta atccaaaaca gcgggaaaga aatgagcgaa ttcgatcaat
    50461 accaaggatt agccgatatt tcattttcag tttataacgt cactcaagaa ttttatgcgc
    50521 aacgagataa aggagcgtcc gtggatgcag caaaacaagc agtccagtct ttgactcctg
    50581 gtacaccagt tgcttcagga acgacagatg ctgatggaaa tgtcacttta tctttaccta
    50641 aaaaacaaaa tgggaaagat gcagtctaca cgatcaaaga agaaccaaaa gacggagtgt
    50701 cagctgccgc aaacatggtt ttagctttcc ctgtatatga gatgatcaaa caagcagatg
    50761 gctcttataa atacgggaca gaagaactag atactatcca tctctaccct aaaaatacag
    50821 tcggtaatga tggaacgttg aaagttacaa aaatcggtac tgccgaaaac gaagcactaa
    50881 atggagcaga atttattatt tctaaagaag aaggaagacc aagcgtcaaa aaatacatcc
    50941 aaagtgtcac agatggattg tacacttgga caacggatca aaccaaagcc aaacatttca
    51001 ttactggtca ttcttatgac atcggcaaca atgactttgc cgaggcaacg attgaaaaag
    51061 gtcagttgat cgtcaatcat ttagaggttg gaaaatataa tttagaagaa gtaaaagctc
    51121 ctgataatgc ggaaatgatt gataatcaaa caaacacgcc gtttgagatc ttggcaaata
    51181 gtcaaactcc tgtagaaaag acaatcaaaa atgatacgtc caaagttgat aaaacaacac
    51241 ctcaattgaa tggaaaagat gtcgcaatcg gagaaaaaat ccaatatgag atctctgtca
    51301 atattccttt aggtatcgct gataaagaag gaacacaaaa caagtacacg acattcaaac
    51361 tcattgatac tcatgacgcc gctttgacat ttgatcatga aacttcagga acgtatgcgt
    51421 atgtcttgta tgatggggat aaagaaatcg atccaacaaa ttattctgtc actgaacaaa
    51481 caaacggatt cacggtttca gttgactcga attatattcc ttcactcact cctggtggta
    51541 cgctgaaatt tgtttactat atgcatttga acgaaaaagc agatccaacc aaaggatttt
    51601 ctaaccaagc aaatgtcgat aacgggcata caaatgatca aacaccaccg tcagtcgatg
    51661 tcgttactgg gggcaaacga tttgttaaag tagatggtga cgttacatca gatcaaacac
    51721 ttgctggagc agaattcgtc gttcgtgatc aagatagtga cacagcgaaa tatttatcga
    51781 tcgacccatc cacaaaagcc gtcagctggg tatcggcgaa agaatcagca acggttttta
    51841 caaccacaag taacggttta atcgatgtga caggtctaaa atatggcacg tactatctgg
    51901 aagaaacgaa agcgccagaa aaatatgttc cattaacaaa ccgtgtagca tttactatcg
    51961 atgaacaatc ttatgtaaca gcaggacagt tgatttctcc tgaaaaaata ccaaataaac
    52021 acaaaggtac acttccttca acaggcggta agggaatcta tgtgtatatc ggtgcaggag
    52081 tagtccttct actgattgct ggactgtact ttgctagacg caagcacagt cagatttagt
    52141 ctttttgcaa gaattggaaa aaggagcagc ggattacgca aatgctcctt tttcttttat
    52201 tatcaaacag tcatcacaag gagaacacga atgaaaccaa agaaaaggaa acttctagat
    52261 ctgctgatgc ttttcttgct tctctcagga atagggatac tagcttatcc atttgttagc
    52321 gacgcgctga ataattatct ggatcagcag atcatctctc attatcagca acaagcggtc
    52381 aaagaaaatg aagaagtcat ggctaaaatc caagaaaaca tgacaaagaa aaaccagcaa
    52441 ttagcaaaag aaggtggaaa tccaggtgca gatcccttta ctaagaaaaa agaacctgcg
    52501 aaaaaggata gaacctattt tgagaaacat acgatcggta ttttgacgat ccctgagatc
    52561 aatgtccatt tgccaatttt tgatgaaacg aatactgtgc tgctagaaaa aggtgcctca
    52621 ttattggaag gaacgtctta tcctacgggg ggagaaaata cacatgccgt tatttccagc
    52681 catcgaggac tgcctcaagc aaagttgttc acagacttac cgaaactgac tttaggtgat
    52741 tctttctata tcgaaatcaa tggacggaca ctcgcttatc aagtcgatca gatcaagacg
    52801 gtagaaccga ctgatacaaa agatctgaga atcgaaaaag ggcaggattt cgtgacactc
    52861 ctaacttgta caccttatat ggtcaacagt catcgcttac ttgtcagagg gcaccgggtt
    52921 ccttatcatg caaaagaagt tcaaaaagaa gtacagaaag tatccgagaa caaacgattt
    52981 ttcctgtatg gattgttagc tgcatgtata ctgattttcc tccttcttat atatcttttt
    53041 cgtcaatcgt tcaaaataag aaacagaagt tagctattcc tcatttctat aatatatcta
    53101 ttcgaatcgt tccgtaaaca aacaggctga ggtttgaaaa agaaaacttc agcctgtttt
    53161 tatgatgata tcaaagagag cctatcaaac actatctttc ttattactaa atgaggatct
    53221 tttcttaagt tttccgatat tctttaagaa ggcctttttg catcaattct tgtttgtttt
    53281 tcaagttgcg gatattctct tcaaaatttt gagaatatat cactgagaat aacgtattta
    53341 atacgtaatc gaaggcaatt tgagaggaga aagtcgctac tttagcaaaa tcatattcct
    53401 cctgcgatat aacaagacac atatcagcct gtttggccat ttctgaatgc tgatttccgg
    53461 taatcagcaa ggtaggggcc tctacatgct tcaaataggt cattattcgt tcataatgat
    53521 ggatgcgacc gctgtagcta ataaaaatgg cgcaatcctc gctagtcaaa ttggctgcat
    53581 tccaagaaga atcactgtat tcttcagcca tgatcaaaaa cttattcaac ttgaccattt
    53641 tattctggaa tttccttgcc gtgatttgag aatcaccttt tgcaaaaaga aagattcgct
    53701 gtgccttgtt caatacttta ccgatttgat ctaaatcttc atgttcaatc tgcaattgtg
    53761 ctttttttat tgactggatc gataaatccg ccattttctt tgcaatttgc tgagtgctat
    53821 cttcacttga aaaaggaaaa ttaggatcaa tcgtttgatt gaactgagca atcaattgat
    53881 gaacttcccg ccccagctcg aaacggaatt ctttaaatcc gctgaatccc atttttttgc
    53941 ataaacgaat gatagctgaa tgggaagtat acgtagcttt tgctaattct tcaatcgtca
    54001 tgtagactgc agcttcgata ttcctacgga tgtaatcgac aatccgtttc tctgtcgtgg
    54061 taaacaactc ttgttttgtt agtttttctt caataagcat gatctgacct ccaaattcta
    54121 tcttactgat aaacacttgg ttccgctact atctttttga tttttatcct tactttgaac
    54181 attaatttca ccaaaccgaa tgataacaag tgtttttctg aactttttta ccagaatagc
    54241 tattataaat actttctgcg cttttctagg atatactgaa aacatcaata aaaggaggca
    54301 ccactcatgc ttacaatcgc ttatcttggc aatggaaaga gtacaaatcg ctatcatttg
    54361 cctttttcaa gaaaacttac gaacaaaatc aatatcaaaa aaattttttc cccatctgaa
    54421 ccagtgtgga aaacattcga agacatcgaa tatactgatc agctgtctga tctttgggat
    54481 gatcctgaga tccaattagt tgtcatcact acacctagtc aatttcatta tgaatatgga
    54541 aaaatggcac tcgaacatgg taagaatgta ctagtagaga aaccttttgc agaaacgtca
    54601 aaggaagcag aagagttatt tgcattagca aaagaaaaag gtctatttat ccaatgttac
    54661 caaaacagac ggttcgattc tgacttcttg acagtccaaa aagtaatcga aagcggaaaa
    54721 ttaggggata ttttagaagt agaaatgcat tatgattatt tcagaccgga agtaccagaa
    54781 aatacgcatg aattttctac tgcgaacagc tatttatatg gacatgcttg tcatacagta
    54841 gatcaagttc tttcttattt cggcgaacca gatgatatca gctatgatgt acgtcaactt
    54901 ttagggaaag gacgaatgaa tgattacttc gatctggatt ttttctatgg aaatatgaaa
    54961 atttcgataa aatccagtta tttccgcatc aaagaacgtc caagtttcgt cgtttacgga
    55021 aaaaaaggga tgttcgtgaa acaaacaaaa gatcgacaag aagaacatct gaaaatgttt
    55081 tacatgcctg atcatgaaga ctttggtatc gatttgcccg aacattatgg tgtattgacc
    55141 tatatagatg aacaagggag ttatcatgaa gaaaaagtaa tttccgaggt tggtgattac
    55201 agccgtgtct acgaagggat ttacgagaca ttagttaatg gcgcagaaaa agtcatcaaa
    55261 gatgaagaaa caatcttaca aatgaaaatt ttggaagaag gaatcaaaag cattcaagaa
    55321 tcagaaggaa aaatatgaca tatttgtaag aatcgggttt gttttttgac gcataaactg
    55381 tcttattgta cgatgaaaaa gtatagaact actaggagga accaacatga caaattatga
    55441 cggtgaaaaa cacaaacgat taagaggttc aatggcccct aaaacagaag aagaagtggc
    55501 tttagcagga actaattctg gtgaaacaga agacaaagaa gaatcattag ctggcacaaa
    55561 ctctggtgaa acagaaaata aagaaacagc tttatctggt tcaaattctg tagaagagga
    55621 aacaaagcct aatgagccca atcactttga aaaagatatc gaaaaagcgg ttgaagatgt
    55681 caaagaaaaa tttgaagaac tgacctcaaa aaataaacat gatcatcatt gatcggacga
    55741 aatcaattca taaaagcagg agacatttgg tcacctgctt tttttctgta tccattcgat
    55801 agaaatcgtt cggtacagtc gtaaaaaaaa tccaactgta agttgtacca aacacccctc
    55861 gatttaaaag cgctaccata ctagaatata agtaggaggt gaacagcgct gtgaactaca
    55921 aaattagtta tctcctgaaa acaccaagag aaatcagtct agaaaaagca gcagagatca
    55981 tcgaagtaaa tacaagcgag gcgatcaaac aattaactcc ttttgccgaa tatgtggatg
    56041 atacccgtat ccgtataaag aaattccgag caatcgactg ggcagaaaaa tttttggata
    56101 ttactcgatt tgaagctgtg tataccgaac aagagcgaag agatcttctt tatttgatgg
    56161 tttattcgac tgcaacgtct gtttctgttt ttcattttca agattttctt caggtttcta
    56221 aagggacagt cctgtcagat atcaaaaaga taagaaatga attagaagtg gaagattgtc
    56281 agctgtcgta tactaggaaa actggtttta ggctgctagg gtcagaagca atattaagaa
    56341 gagaagcaaa aaatcgcact gctcgtttga tgcagtcact tgctggaaga tttgggctca
    56401 cgtatttaac cgcaaccctt tccattgatt attacgccaa agttagagat ttgctagata
    56461 aaacgatttc actatcagat cttcgattcg caccaaatcg aatggatgaa ttagcttatt
    56521 atttgagttt gacaaccatt cgaattggaa aaacatccat ggaactggta aaagatgctg
    56581 aactgttggc ttctcttgca gtcactgaca ttagtcgaca agtattgact ggccttggct
    56641 attccgaaca tcaaaattcg gagattatat ttttctctct ttgcttagca tcgatcgttg
    56701 aaggagatgt ccaggagcct gcacttgatt ttttgctgga atgttcttca gaagtcatcc
    56761 atcgcatgga attcctgatg gccgtagaat ttgcttcgtt tcgtgaatta ttgatgaatg
    56821 tcttttatca cttggtccct gcctattttc gtatttccta ttctttttat ttacctaatg
    56881 tgatgatcga tcaaattaag catcaatatg cttccatcta cgaaatgaca agaaaagcat
    56941 tacttccgtt agaaagacga atagggaaga gcatccctga agaagagatt ggttttttca
    57001 ctatactatt tggcggagag atcagaaaag cggacgcaga agaaagaaat agaaaaataa
    57061 gagccgtgat cgtctgtcca agtggcatta gctcttcatt gatcttaaaa agtgaacttc
    57121 agcaattatt cccgatgatc ttttttacag agactaattc agctgaccgc ttacacgagg
    57181 ttgacgaaac ttcttacgat attatttttt ctactgtacc tctcacactt actgataaga
    57241 aaaagtctct ctatgttctg catccaatca tgactgactt ggaaaaaaat cagctgatca
    57301 atcaagtaca aagagattgg ctgataccag gattttcaat gccttctgcc caagaattgt
    57361 tgtcggcgct actgccttat atcgaattaa aagaaggcgt agacgaagaa aagctctatc
    57421 aagtattaaa tcgtaaaatg aacaaacttt tagaaaagaa ggaggatcaa cgtcctatgc
    57481 tatctgaatt attaacagaa gaaatgatcc aattatcgga tcaaccaaaa aactggcaag
    57541 aagcaatcac atttgccgca cagccgctat tatcagaaaa taaaatagaa gaaagttata
    57601 tcaaagctat gatcgagcgc gtggagcagt atggtgcgtt tatccatatt ggcgatcaca
    57661 tcgcattacc tcatgctcgt ccagaagatg gcgtaaacga agtagggatg tctttgctga
    57721 aacttcaaca acctgtggat ctgcttgata atccagccca tcccattagt ttgttcatct
    57781 gtttagctgc tatcgataat gaggcacatc ttcgggcttt ggcaaattta acaaagctac
    57841 tttctaataa acaaaatctt caagatttat tacacgcatc aacaaaagaa gaaatattag
    57901 cgatcataaa aaaaggagac gaatgatatg aaaatttttg cagtatgtca aagtggatta
    57961 ggaacaagtt ttatggtaga gatgaacatc aaacaagcac ttgcagatct tggagcagat
    58021 gtggacgagt ttgaagtaag tcatacggat gttggaagtg tatctagcga tatggctgat
    58081 tatttcttca ttgagcattc tttgaaagat acgctaactt ctatacctga tgaaaagctc
    58141 gtacctttgc aatcgatcat tgacagcgaa gaagtaaaag aaaaagtaac gaagatcttg
    58201 tcaaaagaat aaaaatgatt caagggagga actatcaatg gataaattgt tgaatttgtt
    58261 tgtgtcaatc gtcagtcagc cctccatcct cgtttctttg attgcactgc ttggtctcgt
    58321 tcttcagaag aagaaatttt cagaagtgat ccaaggaact atcaaaacat ttgtcggatt
    58381 cctagtactg atcggcggtg ctggtttaat cagtaattca ctaacccctt ttgcttcgat
    58441 gttccaaaca gctctgcata ctcaaggagt agttcctagc aatgaagcag tagtggcgat
    58501 tgctttgcaa caatacggta cacctactgc tttgatcatg ctagtaggga tgatcgtgaa
    58561 tattttactt gctagattca caagatataa attcattttt cttacaggtc aagcaatgct
    58621 ttatgtttct tgtctaacag cagtcatttt agtaagcgcg gggtttactg caaatttgtg
    58681 gatgatctta ttaggcgggg tcttcgaagg aacactatta actattacgc ctgctctttg
    58741 tcaaccgttt atgaagaaca tcactggaaa caatcaagtt gcgttagggc ataccggaaa
    58801 tatcggttat gcttctagtg gatggatcgg aaagtttttt ggaaacaaag accgttctac
    58861 agaggacata aagattccta aaagtttcag ctttttgcga gattctactg tatcgatcat
    58921 gctcttgatg tcgatcgtat atgtcgcact tgctttgtta gctggtcctg gatttgttga
    58981 aagagaacta agcaatggag acaacgctgt aatctttgca ctgatccaag ctggaacatt
    59041 cacagctggg tttgttatcg tccttcaagg ggttcgaatg gtgttggcag aaatcgttcc
    59101 agcattccaa gggattgcta aaaaactcgt acccgattct aaaccagcat tagatgttcc
    59161 tatcgttttt ccatatgcac ctaatgctgt attaattggc ttttttgtta gcttctttgt
    59221 aggaacacta tccatgtttg ctatgatagg cttgcaaact acagttatca ttcctggggt
    59281 agtaggacat ttcttctgcg gagctgcttc tggtgtcttc ggcaactcga ctggcggtag
    59341 aagaggggcg gtattaggtt ctgctttcaa tagcttattg attagttggc tgccattatt
    59401 tatccttcca gtattaggag atcttaaaat cgcttcttct acatttgccg atactgatta
    59461 tctagtacca ggacttgtac ttgggaaact aggagaatac ggtcaaatag ccttgacagt
    59521 tggaatcatc gctttctttt tacttgtcgt attatctagc tttctttttg ccggaaaaag
    59581 aacaaacatt gtttctaaag aacagaatta agcaaacttc aaaataaaaa ttgcttggac
    59641 aatatcagaa gaagatttaa agtcttctga tatgtcgcaa gcagtttttt gttttatgca
    59701 tttatttttg aatagcttgt taagacaagt ggctgttttg ctagaagagg ataactttta
    59761 ccattcattt tcactgttcc gcctgttcta tcagaagtaa tcacgatctt atcttttgtt
    59821 ttttcaagcg aaatctttcc atctttgata ggtagcgtac aattcagtgt ttcaaatgaa
    59881 tctagattag gttgtatcat aaattcttca taacctggct ttgttggata caaaccgata
    59941 aaatagcgtc ctaaaagata gattgggctt gctccccatg catggcataa gctttttcca
    60001 taaggatctc catacatact gtacttttca tttcctgttt gagtagggtc gtattcttcc
    60061 cagaaagtta ccgcatcatg agataacatc cctccccaat aatcaagaat cgtttggtag
    60121 acgatattcg tctgtccgag tttgcataaa gcatcttgtt catagaattt gaaatatgga
    60181 gtagtaatct gtgtgatagt gtcattcaat aagacattct ttaagatcga ttgctgttgg
    60241 tctttgttag caaaatcgaa taaaacagcg aaaatattcg catgacgcgt cacatttctt
    60301 ctcccagatt catagcaatc gataaaagcg ccaagttctt catcccaaaa gtagtcaaat
    60361 aggttttgtt tcaatagttg atatttttct tgatagagat gagtatgata attttcgttc
    60421 tctccatatg tttcagctaa gatatctccg caagtgatca tggaatgata ggctttcagt
    60481 aataagactt gttctgcagc aagcgttcct tctttatcca tttcagacca atcaataaat
    60541 atccaatctt ttgctctccc ataaataaac cctaattcat tcgtttgctc catcaagtat
    60601 ctcattaatg attgcatctt agggtagacg atgcgtaaaa attcatagtc tttcgtcatc
    60661 atatagtggt tttcgatacc aatcaaccac aagatcgaat agtcaacgat agtattcata
    60721 tgctgcttga tttcattttg tcctcttaat gctaatatcg ttcgtttatt gatttcctcg
    60781 tcaaaaaaaa gatattgatt gatcatgaat gattgatacg catctccaga ccaaatccag
    60841 cgatctcgtt tgataccgtc gataaaaaac aaatcactac ataacttgaa ggtttcttct
    60901 gatactgacc agatctgatt cagtaaagaa gaatcgcttg aaaaagcagc ttgtttcgaa
    60961 agagataaaa attcatgaaa aacttgaagt tgaaccattc cagtagaaac ctctggtata
    61021 aacaaatagc gaaaagcgcg tttacgagct ttcatttttc ttgtcattcc tatttgctta
    61081 tagtagcaat aatcgatatc catagcttct acatcggact ctccataaca tagagtgata
    61141 gattcgatat ggtcatccag agatattagt tctacacagc cattcactgc tcgcccaaaa
    61201 tcgaataaga ttcctccgcc aatctccgtt tcagtgactg gatggacgca ttctttttga
    61261 taatagattt gatttggatc ttttgcgata tcgacataaa gcggactcca gccagctgga
    61321 tattcttcaa taaaattact tgctgtccag ccaatatctg attttacttc gtctccctcg
    61381 ataaacatcg ctggtagccc tgatgtatgt cctacaaaga tacgaatttt tgctttacca
    61441 gctggacaag tcagccattt tccacatgga tgtttttgtc cattaatttc tacataccca
    61501 actccttgga tcgtcacttt aaacatggaa ggttgatcaa gaaagtaata tctcttaaac
    61561 ttgacgttcc gatggcaatc atccatgtac cagtacgctg gccagtcaaa acctcgttct
    61621 tcccgttgga aattttgtag cagtccatgg cggatctcaa agtctccagg ataccaaagc
    61681 catctatctt tatgtttttc atttgtcatt ttacacgacc tccatcaaat ttattttgtt
    61741 gtcaattgtt cttttatttc ttcatctact cttatcactg tctttctatt cgtcctgtgt
    61801 actactagaa ggctaataag cgcgctaatc aacagcaaga tactgccatt gacgaaaaca
    61861 gcaagtgttt tactcccaag agaagcaaaa gagagaaaga gcaaaccata tggtgcaatg
    61921 aaagtaccta aattagcgcc aaccaataag attgaagtat tgaaactctc cgcgtacttg
    61981 ggcgcttgga tacttacttg gttgaataag taaggaatga ataacgaaaa agaaattcct
    62041 gttaggatcg caccaaaccc aatcacatac aggtttatca cagtggcaat caatacaaag
    62101 ccgattccca tcagtagtaa agaaagtgta agcatatagc gatgtgcttt tgtctgaagc
    62161 cagccaaaac aaactccggc agccatactt gccaattgca tgattgttaa gatattcgct
    62221 gcattagttt cgtttcctag agaataagtc gtcaggactg tggctaattt aatcgataca
    62281 gcattatata ccacgatgac cagcaagaga aaaataccat aactaatcat tttaggattc
    62341 gttttgatta cttgtcttgt tggggctgtg ccatgtgatt gaagttcagt tgtttcaggt
    62401 gtaggaacaa acaaaacaaa caagacaaga ataggtaagc tgatggcgta gactaaaaag
    62461 gtatattgcc aaccgaactg tactagctgt cctgcaacaa atgtcattaa agctgcacca
    62521 aatccttgaa atgcactttg aaaaccaatc atagaggctc gttcatctcc gtgataaaag
    62581 gcagcaatca tgctgactgc caaagaattg atcaatccaa agccaatacc tagtccagct
    62641 cttgaagcta agacaatact gtaactttgt gagaagaggg ggaggatacc ataaatcgat
    62701 gcaatcacta atccgatgat cacagtttgt ttttgtccga ttttcctagc aataaaacta
    62761 ctaatcaaaa caaaaatagt gaccataaaa gagggagcag tcgttaataa ttcaacagca
    62821 gataaagaac gcgtggcata agtagctgac atactgggaa ttgcagacgt gactgcacca
    62881 gcagaagtca aaataagaga gactgacagc agagaaaatt tcatcatctt tttattcata
    62941 agcatgttcc tttcattttt gtaagcgctt atcaaaaata tagtatgaag tggttattct
    63001 ctcaatagcg gaatcacagg aagttcatgt cccaaccata gaaagtcttt atgaaaccaa
    63061 aaatagcttt gcatataata gcagtacagc gtgaactaat aagcgaaaga aaccgcttac
    63121 aaaaaggagg gagtcaattg gaactagctg aattagacat tttttttgcg taacgaagaa
    63181 tcaatcgaaa ctctgcagcg tgaacaagga atgaatatca atgatttaaa cttagcttac
    63241 catcttcaga caccgccaaa aatccaccga ggggatctat ttcaacaggg aaatattcat
    63301 atcagtaaac accgtcgata tgcggaagtt cccgagcata cacatgattt tgttgaaatc
    63361 aattatatgt atagcggtac atgtttacaa attattaatg gagaaaggat agagatggaa
    63421 gaaagtgacg tggtgatcat tgataaagaa gctactcaaa gaatcgaata cacagggcaa
    63481 gaggatattt taatcaacat cttattaaaa gaagaaacct tatctacaga gatattgaat
    63541 catttggcaa gcacaaataa tctactttct tcttttttga taaatgcatc aaaagaaaaa
    63601 ggggaacata cacaattcat tcattttcgc ggcggaaaaa caagtcgttt gacgcatttg
    63661 attgaaaata tgctgttgat ttattttgat acatacagtc ataagatccg ttcgttaaat
    63721 ctctatacat cactattact gatcgaattg actcatttat tagaacaaca aagcttttca
    63781 tcagcagatg gatgcgcaga aaatgagatg cttagagtat tagattatat cgataagcat
    63841 tttacttctt gtacgttaaa agaaacagca gctcattttg gctacaatcc ggtctatctt
    63901 agtaataaat tgaaaatgta ttttgatgct tcctttcaag aactggttct aaagaaaaaa
    63961 ttcgactttg cgttggaact gattcaagaa acgagttttt cgttggaaga aatcgctaat
    64021 cgtatcggat ataaacaaac gacttcttta ttcaaattat tcaagaagta ttcaacatgt
    64081 actcccaatg aatggagaaa taaacattaa gaagaaaatc tttcactaaa gcatatgaag
    64141 gaaaattgtt ttaggtgcga ttgctgtctt attatatcgt tgcagcgaat ttttttggat
    64201 ttattttcag ttctaaactt ttggttgtat tatcagggga tataggtgta aacatttgtc
    64261 atttatctgt tatgcgtatt caggaattcg tttccatcaa aaattataat aacaactatt
    64321 ataattaaca tgttgataaa atgaataaac aagactatac ttttcatgaa agtaacaaat
    64381 agttatgaaa aggagctgtt tattcatgaa aaaaacaagt cgtaaagtag taatcgtcgg
    64441 tacaggtttt gttggaacaa gtatcgctta tgcaatgatc aaccaaggaa tctctaatga
    64501 attagtattg atcgatgtga atcaagaaaa agcagaaggg gaagcattgg accttttaga
    64561 tggtatggct tggggcgatg aaaatgtggc tgtatggtca ggtggctatg aagaatgtaa
    64621 agatgctgat atcgttgtta tcacagctgg tatcaaccaa aaacctggtc aatctcgttt
    64681 agatttagtt aaaacaaatg cgtcaatcat gcgccaaatc gtaaaagaaa tcatgggatc
    64741 aggattcgac ggtattatcg ttgttgcttc aaacccagta gatattttga catatatcgc
    64801 ttggaacgaa tctggtctgc caacatcacg tgttatcggt actggaacaa cattggatac
    64861 aactcgtttc cgtaaagaaa tcgcattgaa attaaaagtt gacccacgca gcgtccacgg
    64921 ctatatctta ggtgaacatg gtgattcaga agttgctgca tggtcacata caactgtcgg
    64981 tggtaaacca gtgtttgaaa ttgtagaaaa agaccatcgt attgcccaag atgaattaga
    65041 tgtgattgct gataaagtcc gtaatgctgc ttatgagatc attgatcgta aaaaagcaac
    65101 ttactatggt attggtatga gtactgcacg tatcgtaaaa gctatcttga ataacgaaca
    65161 agctgtattg cctgtatctg cttatttaac tggtgaatac gacgaaaaag atatctttac
    65221 aggtgtgcca tcaatcgttg acgaaaatgg tgttcgtgaa gttgttgaat tatcaatcaa
    65281 cgaagaagaa aaagcaatgt tcagcaaatc aacaagtgct ttaagagaag tattgaacac
    65341 tgtattataa gaaacaatga atatgaacta aaaatcctaa aactgcttaa cagctgaaat
    65401 agctgttaag cagtttttta ttgatatcta ataggtaagc aaataaacag aatggttaaa
    65461 attaatcggt ccgaaaatgt atccgtacat atgaacatat gaaatttaat ctgttgatta
    65521 caataagcga atcgttatca atgtttatct gtggttaatt aaaaaatcct tccgcaacgg
    65581 caaatacaat gattcatatc taattcgttc atatttgatt aactgaataa agaaactggt
    65641 ttttatcatt caattaaacg acaaaaaaag taagaaaaac tgcaaaattt ttttataatt
    65701 ttgcataagc agattttgat ggaaaaatga cgatttttga taaaaaaccg ttcgagagag
    65761 gtttatcatt ttttaaatct actttgtata atatatatta tgtaaactaa aatatataaa
    65821 aaatcagccc actagttcta aaccactatt tcctactatt cactcttttg tattcctttt
    65881 aaatcaat
//
LOCUS       DAZKCN010000003        64572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000003 DAZKCN010000000
VERSION     DAZKCN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.003"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..626
                     /locus_tag="ACG8K3_000131"
     CDS             <1..626
                     /locus_tag="ACG8K3_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9144048.1"
                     /translation="GKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFT
                     LVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFS
                     PLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHT
                     VAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(745..817)
                     /locus_tag="ACG8K3_000132"
     tRNA            complement(745..817)
                     /locus_tag="ACG8K3_000132"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(782..784),aa:Thr,seq:cgt)
     gene            938..1180
                     /locus_tag="ACG8K3_000133"
     CDS             938..1180
                     /locus_tag="ACG8K3_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144049.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(1319..1948)
                     /gene="upp"
                     /locus_tag="ACG8K3_000134"
     CDS             complement(1319..1948)
                     /gene="upp"
                     /locus_tag="ACG8K3_000134"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9144050.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2082..3029)
                     /locus_tag="ACG8K3_000135"
     CDS             complement(2082..3029)
                     /locus_tag="ACG8K3_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP9144051.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(3182..4426)
                     /gene="glyA"
                     /locus_tag="ACG8K3_000136"
     CDS             complement(3182..4426)
                     /gene="glyA"
                     /locus_tag="ACG8K3_000136"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9144052.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(4601..5626)
                     /locus_tag="ACG8K3_000137"
     CDS             complement(4601..5626)
                     /locus_tag="ACG8K3_000137"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9144053.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(5648..5827)
                     /locus_tag="ACG8K3_000138"
     CDS             complement(5648..5827)
                     /locus_tag="ACG8K3_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144054.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(5820..6659)
                     /gene="prmC"
                     /locus_tag="ACG8K3_000139"
     CDS             complement(5820..6659)
                     /gene="prmC"
                     /locus_tag="ACG8K3_000139"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9144055.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(6652..7725)
                     /gene="prfA"
                     /locus_tag="ACG8K3_000140"
     CDS             complement(6652..7725)
                     /gene="prfA"
                     /locus_tag="ACG8K3_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9144056.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(7728..8312)
                     /locus_tag="ACG8K3_000141"
     CDS             complement(7728..8312)
                     /locus_tag="ACG8K3_000141"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9144057.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(8418..8666)
                     /locus_tag="ACG8K3_000142"
     CDS             complement(8418..8666)
                     /locus_tag="ACG8K3_000142"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144058.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(8657..8887)
                     /locus_tag="ACG8K3_000143"
     CDS             complement(8657..8887)
                     /locus_tag="ACG8K3_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144059.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            8993..9595
                     /locus_tag="ACG8K3_000144"
     CDS             8993..9595
                     /locus_tag="ACG8K3_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9144060.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(9699..10268)
                     /gene="lepB"
                     /locus_tag="ACG8K3_000145"
     CDS             complement(9699..10268)
                     /gene="lepB"
                     /locus_tag="ACG8K3_000145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9144061.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10498..11265)
                     /locus_tag="ACG8K3_000146"
     CDS             complement(10498..11265)
                     /locus_tag="ACG8K3_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP9144062.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(11262..11741)
                     /locus_tag="ACG8K3_000147"
     CDS             complement(11262..11741)
                     /locus_tag="ACG8K3_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP9144063.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(11866..12603)
                     /locus_tag="ACG8K3_000148"
     CDS             complement(11866..12603)
                     /locus_tag="ACG8K3_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP9144064.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            12764..14164
                     /locus_tag="ACG8K3_000149"
     CDS             12764..14164
                     /locus_tag="ACG8K3_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9144065.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14387..15739
                     /locus_tag="ACG8K3_000150"
     CDS             14387..15739
                     /locus_tag="ACG8K3_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP9144066.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            15732..16430
                     /locus_tag="ACG8K3_000151"
     CDS             15732..16430
                     /locus_tag="ACG8K3_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP9144067.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(16492..17643)
                     /locus_tag="ACG8K3_000152"
     CDS             complement(16492..17643)
                     /locus_tag="ACG8K3_000152"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9144068.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAEFIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(17656..18093)
                     /locus_tag="ACG8K3_000153"
     CDS             complement(17656..18093)
                     /locus_tag="ACG8K3_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9144069.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18108..19874)
                     /locus_tag="ACG8K3_000154"
     CDS             complement(18108..19874)
                     /locus_tag="ACG8K3_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP9144070.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20138..20815
                     /locus_tag="ACG8K3_000155"
     CDS             20138..20815
                     /locus_tag="ACG8K3_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9144071.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            20818..21945
                     /locus_tag="ACG8K3_000156"
     CDS             20818..21945
                     /locus_tag="ACG8K3_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144072.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22145..22585)
                     /locus_tag="ACG8K3_000157"
     CDS             complement(22145..22585)
                     /locus_tag="ACG8K3_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9144073.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(22596..24629)
                     /locus_tag="ACG8K3_000158"
     CDS             complement(22596..24629)
                     /locus_tag="ACG8K3_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP9144074.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(24631..26334)
                     /locus_tag="ACG8K3_000159"
     CDS             complement(24631..26334)
                     /locus_tag="ACG8K3_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9144075.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(26428..27378)
                     /gene="manA"
                     /locus_tag="ACG8K3_000160"
     CDS             complement(26428..27378)
                     /gene="manA"
                     /locus_tag="ACG8K3_000160"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP9144076.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(27417..28442)
                     /locus_tag="ACG8K3_000161"
     CDS             complement(27417..28442)
                     /locus_tag="ACG8K3_000161"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9144077.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(28651..29520)
                     /locus_tag="ACG8K3_000162"
     CDS             complement(28651..29520)
                     /locus_tag="ACG8K3_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9144078.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(30224..32116)
                     /locus_tag="ACG8K3_000163"
     CDS             complement(30224..32116)
                     /locus_tag="ACG8K3_000163"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144079.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32113..33840)
                     /locus_tag="ACG8K3_000164"
     CDS             complement(32113..33840)
                     /locus_tag="ACG8K3_000164"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144080.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(33856..34506)
                     /locus_tag="ACG8K3_000165"
     CDS             complement(33856..34506)
                     /locus_tag="ACG8K3_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP9144081.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            34710..35006
                     /locus_tag="ACG8K3_000166"
     CDS             34710..35006
                     /locus_tag="ACG8K3_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9144082.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            35198..35818
                     /locus_tag="ACG8K3_000167"
     CDS             35198..35818
                     /locus_tag="ACG8K3_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9144083.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(35910..37076)
                     /locus_tag="ACG8K3_000168"
     CDS             complement(35910..37076)
                     /locus_tag="ACG8K3_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9144084.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37088..37441)
                     /locus_tag="ACG8K3_000169"
     CDS             complement(37088..37441)
                     /locus_tag="ACG8K3_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9144085.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            37569..38591
                     /locus_tag="ACG8K3_000170"
     CDS             37569..38591
                     /locus_tag="ACG8K3_000170"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP9144086.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            38610..38771
                     /locus_tag="ACG8K3_000171"
     CDS             38610..38771
                     /locus_tag="ACG8K3_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144087.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(39030..39464)
                     /locus_tag="ACG8K3_000172"
     CDS             complement(39030..39464)
                     /locus_tag="ACG8K3_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144088.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(39574..41433)
                     /locus_tag="ACG8K3_000173"
     CDS             complement(39574..41433)
                     /locus_tag="ACG8K3_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9144089.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            41434..41574
                     /locus_tag="ACG8K3_000174"
     CDS             41434..41574
                     /locus_tag="ACG8K3_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP9144090.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(41551..42710)
                     /locus_tag="ACG8K3_000175"
                     /pseudo
     CDS             complement(41551..42710)
                     /locus_tag="ACG8K3_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(43017..44441)
                     /gene="araA"
                     /locus_tag="ACG8K3_000176"
     CDS             complement(43017..44441)
                     /gene="araA"
                     /locus_tag="ACG8K3_000176"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9144091.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(44457..45155)
                     /locus_tag="ACG8K3_000177"
     CDS             complement(44457..45155)
                     /locus_tag="ACG8K3_000177"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9144092.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45112..46773)
                     /locus_tag="ACG8K3_000178"
     CDS             complement(45112..46773)
                     /locus_tag="ACG8K3_000178"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9144093.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            46991..48391
                     /locus_tag="ACG8K3_000179"
     CDS             46991..48391
                     /locus_tag="ACG8K3_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP9144094.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(48643..49722)
                     /locus_tag="ACG8K3_000180"
     CDS             complement(48643..49722)
                     /locus_tag="ACG8K3_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9144095.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(49808..50767)
                     /locus_tag="ACG8K3_000181"
     CDS             complement(49808..50767)
                     /locus_tag="ACG8K3_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP9144096.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(50779..52290)
                     /locus_tag="ACG8K3_000182"
     CDS             complement(50779..52290)
                     /locus_tag="ACG8K3_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP9144097.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(52312..52875)
                     /locus_tag="ACG8K3_000183"
     CDS             complement(52312..52875)
                     /locus_tag="ACG8K3_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9144098.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(52881..53744)
                     /locus_tag="ACG8K3_000184"
     CDS             complement(52881..53744)
                     /locus_tag="ACG8K3_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144099.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(53758..54654)
                     /locus_tag="ACG8K3_000185"
     CDS             complement(53758..54654)
                     /locus_tag="ACG8K3_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144100.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(54829..56118)
                     /locus_tag="ACG8K3_000186"
     CDS             complement(54829..56118)
                     /locus_tag="ACG8K3_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9144101.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56090..57607)
                     /locus_tag="ACG8K3_000187"
     CDS             complement(56090..57607)
                     /locus_tag="ACG8K3_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP9144102.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            57805..58695
                     /locus_tag="ACG8K3_000188"
     CDS             57805..58695
                     /locus_tag="ACG8K3_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP9144103.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(58768..59592)
                     /gene="nadE"
                     /locus_tag="ACG8K3_000189"
     CDS             complement(58768..59592)
                     /gene="nadE"
                     /locus_tag="ACG8K3_000189"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9144104.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(59604..61076)
                     /locus_tag="ACG8K3_000190"
     CDS             complement(59604..61076)
                     /locus_tag="ACG8K3_000190"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9144105.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(61713..62075)
                     /locus_tag="ACG8K3_000191"
     CDS             complement(61713..62075)
                     /locus_tag="ACG8K3_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9144106.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            62298..63050
                     /locus_tag="ACG8K3_000192"
     CDS             62298..63050
                     /locus_tag="ACG8K3_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9144107.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(63190..64113)
                     /locus_tag="ACG8K3_000193"
     CDS             complement(63190..64113)
                     /locus_tag="ACG8K3_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9144108.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64128..64400)
                     /locus_tag="ACG8K3_000194"
     CDS             complement(64128..64400)
                     /locus_tag="ACG8K3_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144109.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18601 a        13294 c        11268 g        21409 t
ORIGIN      
        1 taggaaaaaa aatagaactt aaaaaaggga tattgggcac tattgtcgtt gtactcggtt
       61 tgttagggtt gctatttgcg ccatccatta ttagcatttt ttctttaact atcactggat
      121 ttatagcatt tacacttgtt tttggtaaaa aagatacgaa aaaaatgttc tcgaaacctg
      181 tcgctcctgt caaaaatata gccaaatatt tttttatcaa tgtagtaatc agtgccgcag
      241 tctcggtatt tttgcaacaa attttgaaat ggggtttaac aggcaatcca ataaatgagg
      301 ctttcagccc gttattattt attattttgc ctatcatgat acttggagaa gaattgttct
      361 cagtgtattt tctcgctatt ttttctagta agttcagcat tcctgtcgct agtttcttgt
      421 cagcaatcat ctttggattt atccattaca gtacttatga taatggaaat attttacaca
      481 ctgtagcaca catccttttg atacaagggg tggcaagatt gttattcaac caagctgcca
      541 ttaaaagcaa ctcgatcatt acaagttggg cagttcatgt gatatttgat ttcacagcta
      601 tattgctagt cgttttattc tcctaaattt ttatagaaat aatacatgct ctattgctaa
      661 gaaagcatac tttatttcct ttaaacaaaa agacagatgt atcaatggat taaaaatcct
      721 tgatatatct gtcttctctt ttaatgccag ctatagggat cgaacctacg acctacgcgt
      781 tacgagtgcg ttgctctacc aactgagcta agctggcctt atccaaaaga aattatatac
      841 tctatttggc tgattgtaaa gagtccgatg gaaaattagt ttaatttcat gatatttttc
      901 ataaaacgtg gtataattga tgtgaaaggc aggttttatg aaaatggcaa cttttggaaa
      961 atttgataca actatcgtac catcggaaat tggtgaaaaa ttcaagatca atactcacgt
     1021 aatgttcaca attcacggca agtcgcaaac tggaacgatt tcaaagcaat tgaaaaattc
     1081 cgctgttgtt gagattgatg aaactgcaga aaatgcgaac ttgatgacgc aaagtaaggg
     1141 tgtagtaatc gttaactaca aagaactaaa aaagatttga atttgaccat ctctgcattt
     1201 ttgggatggt ttttttatat gcaaacgaaa gctgtgaaaa agctgttctg catcaaacgt
     1261 taaaaatgac caaaaaagga tgtgacaaaa gttgtgtcac accctttttc gtatttactt
     1321 attttgtacc aaacaaacgg tcacccgcat cccctagtcc aggaacgata taaccatgtt
     1381 cattcaattt ttcatctaga gaagctgtat agatgtcaat atcaggatgc gcttcttgca
     1441 aagcttttac tccctcaggt gctgctacca aacaaacaaa tttaatgttc gacgcgccac
     1501 gttctttcaa tgcatcgatt gccatgattg ctgatccgcc tgttgccaac attggatcta
     1561 ctacaaacaa ctgacgtgca tcaatgtctt ctggaagttt gacgaaatat tcatgtggtt
     1621 ccaatgtttc ttcatctcga taaagaccga tatgtcccac tttagcagct ggaatcaatt
     1681 ccaagatacc gtcaaccatt ccaatacctg cgcgcaagat tggaataatc gctacttttt
     1741 taccagacaa cgttttttgt gttgtttcag tgatcggtgt ttcgatcacg atgtcttcca
     1801 atggcatatc ccgtgatact tcatatgcca tcaacatagc gatttcatcg actacttcac
     1861 gaaaaacctt tgttccgcag tttttgtccc gaataatagt tagtttgtgt tgaatcaatg
     1921 gatgatcgat cacttgaaat ttgcccattt tttaagctct cctttgttct gtcgtttctt
     1981 tttaattgta aaacaaaatc aagtgattga ctagtcaaac tcgccatttt tcctaagaaa
     2041 atccgttttt gaaaagaagt tttcctgcgg acaagcaaag cttattcttt tttcttgatc
     2101 caagaagtag ataatgccat tgctgcacgc gttgcctttg tctgatccat agcatttacc
     2161 atgacaaaat catggatgat tccttggaag cgtacttgtg taacaggaac ccctgcatca
     2221 cgaagtttgc gcgcataagc ctcaccttca tctcttaata catccgcttc tcccgttaaa
     2281 atcattgctg aaggaagatc atttagttca tttatatttg ctctcaaagg cgaagctgtg
     2341 atttcagcac gctcttcttc attcgtcgta tattggtccc aaaaccacat cattccttct
     2401 cttgtcaaaa aatagttttc tccgaattca tgataggaat ctgtatcaaa atcagcgttt
     2461 gtgactggat aatacagcaa ttgcttggat attgggaggc cctttcgttc ttttgttaag
     2521 atcgtcatga ctgtagccat gttaccacct actgaatctc ctgcaacgat tagattctcg
     2581 agatccagat tttttgactt ggctaattta ttcagctgtt gcaacacagc ataattttgt
     2641 tcaattgctg ttggatattt tgcttctggt gatcgactat attctggaaa gacaacgacg
     2701 gaattcgtac gaaccgcaag ttctctgatc aatttatcat gggtttgagc actgccaaaa
     2761 acccagcctg ctccatgaat ataaaaaata actggtaatt tatccatatt cccttctggt
     2821 cgtacgagac gaatattgag ttttccccat tcgccagtat ccaccatcaa gtcttcaata
     2881 tcgacggaat atttgttcac aggtgattct tgtacttctt ctagtaaaac tcttccttct
     2941 tccggtgcta gttcatagat acgcggatga ggcgcatttg catcactgaa tgctactgct
     3001 tccttttcta acgagatacg atcagtcatg acttttcctc cctgagattt agtgtgaaga
     3061 ctaaaattac acctcatata ctagagagca tcaccgatat tgtcaaagaa tccgttttcc
     3121 caaaaaataa acagaaaagc ccaacattga acttttctgt ttactaatgg aatttaaatt
     3181 tttatttttt atgcaacgga aatttttcag ttaattcgcg tacacctgtt ttgacttcgt
     3241 ctagttgagc gttatcatct tttgcttgta atgctttgac aactagttca gccactttta
     3301 cagcatcttc ttctttgaac ccgcgagtag tgattgctgg cgtgccgatt cgaataccgc
     3361 ttgttttgaa tggacttagt gtttcaaaag gaatcgagtt tttatttaca gtgatattca
     3421 cactgtccaa aatgctctct gcttcttttc catttatacc taactcgcga acatcgatca
     3481 acatcagatg attatctgtc gcccctgaaa tgactctggt tccaattgct tgattgaaaa
     3541 ctttgaccat tgcttttgca ttggcgatga tttgttcact atattcttta aatgcaggat
     3601 ctaatgcttc tttgaatgct actgctttac ccgcaatcac atgctctaga gggccgcctt
     3661 gaataccagg aaataccgca ctatttattt tttttgctaa tgcttcatcg tttgtcaaaa
     3721 tcatgccgcc tcgagggcca cgcaatgttt tatgagtggt agtcgttgtg atatctgcat
     3781 aaggaactgg attaggatga agtcctgctg ctactaatcc tgcgatatgt gccatatcta
     3841 ccatcagttt tgcgcctact tcatctgcga tttctctgaa tttcgcaaag tcgatcgttc
     3901 gtccgtaagc actggcacct gctacgatca attttggttg atgttttcta gctaaaatac
     3961 gtaccacatt ataatcaata acttctgttg tcggatcaac accgtaagca acaaagtgat
     4021 aggttttgcc actgaagttg actggtgatc catgtgttag atgtccgcct gcggaaagat
     4081 ccattcctaa gatcgtgtcc cctggttcta ccaaagcgag ataagcagcc gtgttggctt
     4141 gagagccgga gtgaggctgg acgttcgcaa atttcgctcc gaataattct tttgcacggt
     4201 caatcgctaa attttcaacg atatcgacaa attcacaccc accataatag cgatgccctg
     4261 gataaccttc tgcgtattta tttgtcaaga tacttccttg tgcagccatc actgcttcgg
     4321 agacaaagtt ttcagaggca atcagctcca aattatgttc ttgtctttct tcttctttgg
     4381 cgatcgcagc ccaaagatca gggtcaaacg ttttgtaatc taccacaaac aacagctcct
     4441 ttttattttc ctacaatttt atttttccca tcaaaaacaa attgccaaat attcgtatct
     4501 cttctattac gattagttac gcttagtttt tcgttcactg atagaagatt tacttgatag
     4561 cattgtatca tactaaaaga atgcgacaat aaccaattga tcatttttca aaataatttt
     4621 gtcctgctgc ttttttcaag cgattcatgt aagcattccc caatccagtt tctggataca
     4681 cttgtacaaa aatcaaatct aatccaagtg ttggttcatc taatcctctt agacctgcaa
     4741 acaatccttt agccgccgct tctactgagt tgtcgttata catataaaca gctgcagtat
     4801 ctgtccgcac ttgatccgca atttcggggc ttgctatgac acctgcacgg atcttcttat
     4861 tttttgccca ttgaactgca gttgaccaat cgccatctct gaccatcaga acacgtgtat
     4921 cgggtgagta gtgtttgtat ttcatcccag gggcttttgg tgtttcattt tcttttacaa
     4981 gatgctgatc aatagcaaca ggagattcga tgactgcttc gatttgttct ttcgttactg
     5041 cacctggtcg taaaatcata ggcatggctg ttggatcact taaatcaaga acagtggatt
     5101 ctacaccaat tcgcgtagct ccatcatcaa taatcccggt aatttttcct tgtagatcat
     5161 gatatacatg atctgcagtc gttggacttg gttttcccga cgtatttgca cttggaccga
     5221 caagcggaac accggaaagt tcgattactt ctagtgtttt tttgttatcc ggtatacgaa
     5281 atgcagctgt ggataatcct cccgtcacaa ctgagggcaa agtgtctttt ttgatcttaa
     5341 atatcaaagt taatggtcca ggccagaagt tcttcacaat cttctctgct gccggatgaa
     5401 agttgtccac atactctttt acttgttcaa aagaggcgac atggacgatt aatggattat
     5461 cttgcggtct cccttttaca gaaaacactt tcttgacagc gtttggtaaa agagcgttgg
     5521 cgcccaatcc gtatactgtt tcggtaggaa aagcaacaag ttctccttct ttcagtgctt
     5581 cggctgcctc ttgcaattct gtttcatcat atcgtttcgt ttccacagtt tatccacaac
     5641 ccctttttca tacattctta ttttgtctga gtttatcctc aagttttcca ctgtttgtgg
     5701 ataatctatt tgtcaatagt ggataaactt gttgataaac caaaaagtcg tttacatcct
     5761 tgtcaaataa gtgttttttt ataataaaaa aacaaataaa aaatttttca tccacaactt
     5821 caaccataaa cttggatcat tcgttcattg ccaaaaagat ccttttttat cgccactttt
     5881 ttatctggaa atgcttgctg aaatattttt tttactgctt caccttggcg aaatccgatt
     5941 tctaaaaaaa tctttccatt tgattttagt acgcttggtg cttcaactgc tattttttca
     6001 taaatagcta atccatcgtt ctcagcaaaa agggccattt taggttcgaa tgaccggaca
     6061 ctttcatcca tcagttccca ttcattccga ctgatgtacg gcggattaga aataatcaca
     6121 tcaaattgct gatccattac tggtcctaat gtatctccat gataaaaact gattttcgtt
     6181 ccttctttct gagcattttc tttcgctact tctaatgctt cttctgaaag atccacagca
     6241 gatatatgcc aattttttct tgctgctttc aaactaatag caattgctcc agtccctgtc
     6301 ccaatatcta tcacttctaa cggtacacca ggcgtctcgt ctaaacacca ttcgactaac
     6361 tcctctgttt ctggtcgagg gatcagtgtt gcttcagtta cctttaaccg atgatcataa
     6421 aaatctgcat atcctagcaa atactgaggt ggatgatctg caagcagaag tcgcagatcg
     6481 gtttcgatca accgctgttc ttcttctgaa atctcttcgt tcatatgaag gagccaatca
     6541 agttttttcc attgttttcg ttccaaaaat aaaaattgga tactatatgc ttctttccca
     6601 tgatcctcta aaaaagaaga agcccgtgac aggacttctc gataggtcat tttacccatt
     6661 ttgcatagct tccagttttg atgtttgatc atacatgatc aatgcatcaa cgatctcatc
     6721 taatttccct gctaaaatct gatctaattt ttggatagtt aatccgatac gatgatcagt
     6781 cacacgattt tgcgggaaat tatacgtccg gatccgttct gaacgatccc cagttccgac
     6841 tgctgatttt cgattggcat catactcgct ttgtgcttct tgctggatct tatcgtacac
     6901 acgagcacgc aagattttca ttgctttttc acggttcttg atttgagaac gttcatcttg
     6961 catagcaaca acgatccctg ttggtaagtg agtcaaacgt acagcagatg ctgttttatt
     7021 gacgtgctgt ccgccagcac ctgaagcatg atagatatct gtacggatat ctttgtcttc
     7081 aatatcgatc tctacttctt cagcttctgg catcacgaca actgttgcgg tggaagtatg
     7141 gatacgccct tgcgattcag ttgaaggaac acgctggacg cggtgcgcac cactttcata
     7201 ttttaatttc gaatatacgt tgtctccggt gatcatcatg atcacttctt tatatccacc
     7261 gattccggta acgttagctt ccatcacttc tgttttccag ccttgtgctt cagcatactt
     7321 ttggtacata ttgaaaagat cgccagcgaa taatgctgct tcgtctccgc ctgctgctcc
     7381 acggatttcc atgatgatat ttttatcgtc atttggatct tttggtaaaa gcaagatctt
     7441 gattttttct tctaagactt ctttttcttt ttttaattca gataattctt cttttgctag
     7501 ttcagccatt tcatcatcca gtttttcacc aagcagttct tctgtatcgg tgattccttg
     7561 tactacttct ttataacgac gataaacttc gactgtttcg cgcgtgtttg cttcttcttt
     7621 tgaaagctgc ataaaacgct ttgtatcaga aatcacttct gggtcactta aaagttctcc
     7681 aagctcttca taacggtctt caattgcttg taattgatcg tacatgctta ttctccttct
     7741 gtaatcattg atgggtgaaa gtaatgtttt cgacaaacgg ggtaataagc ttcatttccg
     7801 ccaatttgca cttgatcgcc ttcataaaca gggtttccat caatataatg taaattcatg
     7861 attgcttttt tgtggcagaa ccaacagatt gttttcattt cttcgatttt gtctgcataa
     7921 agcaataaat attttgatcc ttcaaataac tcgtttcgaa aatcattttt caaaccaaat
     7981 gccataaccg gaatatccaa ttcgtccaca attctcgtaa aagcaatgac atgtttcttt
     8041 tttaagaatt gcgcttcatc gatcagcaca caatagggtt tgctttccat tttctgaatt
     8101 acgtcaaata cgtctgtttc ttcaaatatc ggcatcgcat tacgtttcag tccgattcgg
     8161 cttgaaacaa cgcctaaacc atctcttgta tcaataccgc ttgtcattaa gacaactggc
     8221 ttattttgtt cttcataatt atgtgcgact tttaggatct cgatcgtctt gccgctattc
     8281 attgcgccat acttaaaaaa tagttgtgcc atgctctttt tcaccttcca gtttgttcct
     8341 atggtattat acttgatttt cagcattttt tacagtgaga attttcatca agcacagatt
     8401 agtattaaat ctattgctca cttttgttcc aattttttga caagaactaa acatgggttc
     8461 cattcatccc ataaatttgg aaaaacttcc aaccttgaaa atcctaatga ttcgtagaaa
     8521 caaatagttt gatcatatat agagtagtgc ccctcgtcta cagtcttcac ttgcaaatat
     8581 ttgtaatgtt ttgctgcgta accttctgct tctttaaaaa gttttgttcc taatccttgg
     8641 tgatgatagt ctttttttat tcccatacaa tagaatagat ttcggcagta tcttcacttg
     8701 tctgtgaaag agtgataaag cctaaatatt cttcttttct tttttcacac cataaaggta
     8761 gtttacttga ttcctcgata tatttttccg ttgattcagg aagcccaaac cactcaggca
     8821 agtctagtaa tatttctcgg acacactttc ctttttcctc tctatctttt acaaacgata
     8881 tcggcatatt ctttctagat tctccctttt tgaattgagt atagcatgct ttctttcttt
     8941 tcacgtgcta taataagtta aaatttcaaa gttttacgta aggagctatt tgatgacaaa
     9001 aaatattttt gattccatcg ctaaccatta tgattcccct gaccgtcaag cattagccaa
     9061 gattattcgc gaagaactaa ctacttatct accgcgcgac agccaccaaa aagtcctgct
     9121 ggattacgga ggcggaactg ggcttgttag tttaccgctt gcggaaagat tcaaagaact
     9181 gatcattgcc gatgcttcag aaactatgct gaaaatggca gaagaaaaga tccaagcagc
     9241 agatctgaaa aatgtccgaa cgatccacgc tgatgcttct gtcgaatttc ctgctgtcca
     9301 agctaatctc atccttctat cacttgtact tttacacatt cctgatacgg agaatattct
     9361 tacaaaactt tatgaaattt tagctcctgg cggtcaattg atcatcgtag actttgacaa
     9421 gaatgaacaa attagtcatc cgaaagtcca taatggcttt agccaagaag aattgaataa
     9481 tcgattggaa aaaacgggct ttgtctctgc tactagtcat accttccatc gtggggaaaa
     9541 gctatttatg aacaaaaacg cttcgctttt cttatccatc agccaaaaag cttaaaatga
     9601 gattgcaaaa aaagtgagca actccaagaa ataagaagga attttcggaa aaagaagctg
     9661 tgacaaaaaa catgtcacag cttcttttta ttttttcatc acatgatttt aatttctgaa
     9721 agaggccaat aacggaaaac aacttttcct tggatcatat ctttgtcgac taacccaaac
     9781 attcggctgt cttttgaaat caagcggtta tcgcctagaa caagatactt tccttttggc
     9841 acagtatatt caaagtcact cgtaaaggtg gatgaacttt ctgcttgtgc ttggaatcct
     9901 gagctataag catattctcc ttgtaactta tcttctgaaa attctttttt gaactcatcc
     9961 agataaggtt catcatactt tttgccatta atggtcaact ggtcattttc catcttgact
    10021 gtatctccag gcataccgat gatccgttta acaatcatcc gttcttcatc ccccggttcc
    10081 tttgtagtaa taatatccat tcgttcatag tttgagatct tagatgaaat caatcgttgt
    10141 ccatcactta atgtagggtc catcgaatgc ccgtttacag taactggtgt aaacacgtac
    10201 actctagcta ataaaatagc cgctaataca aggatcaaaa tcccccaatt tttcaaaaat
    10261 tctttcacag cttacctcct acttgtattt tcctcgttat ttattacgtt ttttcacaat
    10321 acttcgttgt actttcttta ttctacaatg tttataaatg aaaggaaaga ataagtgatg
    10381 tgttcgtctt atctttctcg ttatataaaa aaggaacgtg acaaaagacg tgtcacatcc
    10441 cccttattcc gaataaacgt ttttatcgca accactgata cacggcgttt catcacatta
    10501 tgaatgtctt cttctttggc tacgactata aaccactggt tcttcctgcg actgtgcctt
    10561 ttttgcaatc aatttcagac gataaggctc atgatagcgt aacccgtctt tgacaaaatc
    10621 accgccgacg atgaacaatc cgacaggttc caatgtgtct tctgttgagt caagtcgaat
    10681 ggtccctact ttttccatgt ccgagtagct tcgtccaata tttagaacat actctttctc
    10741 tcctgttttc ttgacatagt aataaggata caatgtattt ctatcgacca gaaatccatc
    10801 catgcgaccg agttcatttg ttttgatctc accatttcca atcaaatacc gcatattctc
    10861 ggctaatttc aattgataat cttcgtcata agaaaccatg acctcttgtg tattcacttt
    10921 atcgatcgaa tttccattat ctatcgattc ttctgtctgg atgttgaacg cttcatcagg
    10981 taaatacttc acaaaaagct gagtgacatc ggctttcgtg tttttatcta aaaagtaata
    11041 aaatacattt aaaacagtaa aataaacaag cactagaaat gcaagtccat ataagaaaaa
    11101 ttgaagatag ttttggtttt gccaaagtcc ataaacaatt ctcaataaat aaagcgtggg
    11161 aatcacactt aaaatcgtat atccacggtt aagatattta ggactgatgt ctaaatagcc
    11221 aaggaattta tgcaccccgt tgataaggtc tagtaaaaat gtcattgaga atcacctgct
    11281 tgctggttat acgtttgggt atttcctgaa tcttggtttt gctgtgctgt acgtgctgca
    11341 tcatcatttt gcgtgttatc atctacagtc gtactgcttg gtatcgtttc ggagctagca
    11401 cttgtatccg gacttgtact tggattggtt gaatccgatg gagtgccatc tggtaccgta
    11461 ttacttgttt cgcttgagct gtcagttgta tatgtctcat catacgacga actagttgtt
    11521 gtatcgtccc atgtacttgt cgtagtggtt tcattcaaac tttccgttgt tgttggttca
    11581 gatgtcgaga ctgaagtagt tggtgttgtt tcttctacag tactagaact tgaacttggt
    11641 gcacggtagc tggaactatt cacaatacct gtagtagtcg cgacaatcgt cgagattgtt
    11701 aatacagcga ttggcttcaa aataggtgct acttttcgca tcttatctct tccttcattg
    11761 aaatttattc ttgtttcctt actttactac ggacaaatta tacagtctac cttatttcta
    11821 ccataaattc tttatttttt tcttacgttt ttccgaaaga tttctttaat tttcctcaaa
    11881 aactacggtt gatgttttta agtaagcttt tgcttcccga ttgatttctt tcagatcgca
    11941 tccttgctgc ttggcagcga acacgcaacc acaataacat tggcgataaa tatcatactc
    12001 tttgcacatt tgcaaagagc gttcatagcc tttgtttttc ttgaaatcac ttggcaagta
    12061 ttgtgtcccg taaatctttt ggatatccat tccgatctga ttgatcaaag ctgcattttt
    12121 ctttggcgag atggtcaggg cacttccaaa gtaatcgtat cctagttctt gcgctttttg
    12181 cgcaacaaga tctaatcgca tattgaagca tgcactgcag cgagcaccac cttctttttc
    12241 gttttcaaga tggtttgtta aaaccatttt ggtgaattca tttggacggt attcatcaac
    12301 gattagcgca acatgatggt cagttttctc attaaattgg tctacaaatc gtttttgttc
    12361 atgtaatctt cgcaaatact cacttttagg atgaatattc gaatttgaaa aaaataacgt
    12421 gatctctgca tattgagaca tgaattccaa tgtgtgcgta ctgcaaggag cacagcatgt
    12481 atgtattaat atcttaggct ttacttcttc tttctcccat tgcatgatca tttttttcag
    12541 aactgtatca taatttattt tttgattttt cattttttct acgatttgtt gagcattaat
    12601 caatttgtta ttcctcctcc ttcttttttc taggattttc cttattataa gccatttcta
    12661 ttttaatcga aagctgtatt gttatctgtt tgataatttc tttgagtatt cctaattccc
    12721 cttgtatact ggtagaaacg actattgtca ggaggatagt actgtgcatg ttgatttatt
    12781 ctttcgaagg ctgcagcggc gaggacagaa gtttgctgcc aatcatttct catcgatcca
    12841 aatcatcgtt ttttattata tattgatgac ggtcttatca ctcgttctat tttatatgcc
    12901 gatttttcgc gaaccagata gccatgtttc ttttgtggac atgctgttca tggcaatcag
    12961 tacagtgagt gtgactggac tatcgacttt tgatatcaat tctgtgttca atgataacgg
    13021 tattatactg ctggaaatat tatttcaagt tggtggtttg gggatcatga tgatttctac
    13081 tgcttttgtc attttttcta aacgacggat cacactgaaa cagcgacagc tgatcatgac
    13141 ggatatgaat caaccgcgtt tgtctggtat cgtccgactg attcggatca cttttgctat
    13201 tttaatctgg tttcaattac tttttgggac attcttttct atttattttt attacagagg
    13261 ctattttgat cgttggcgtg atgccgtttt ttacggtttt tatcaggcga tatcggctgt
    13321 cacgaactct gggtttgacg tcactgggga ttccatcaag ccctttgcac atgactattt
    13381 tttcttgatc ctgatcatgt ttttgatttt tgtcggagga attggttttc ctgtattgat
    13441 ggagtgccgg gaatggctat tatacaaacg ttccaatgcg aagcttccgt ttcgtttttc
    13501 cttgtttacc aaattagctg tgttagcttt tgttatcttg tttgtttcgg gaactgtctt
    13561 gatttatcta ttggaaaagg accatttatt ccaagattcc aatatgagtg tcaaatggat
    13621 caattcgatg ttttattcga ttacgactcg taatgctggt ctgcagattc acgatttggg
    13681 tgattttcaa atcacaacac tgatcatttt ttctttactg atgtttatcg gatgcagtcc
    13741 tagttctgta ggcggcggga ttcgaacaac tactgtagca atcatcggtt tgtatctata
    13801 ttccttcctc aaaagtgaag ataacatcaa tatttttgga cggagaatcg atcaagatga
    13861 tgtgcgaaaa tcagtcgttg tctttatgct ttctcttggt atgtgtttct tttgtatcgt
    13921 ttttttatct gctacagaag aacaaacgct gatctcgatc atcatcgaag taacttctgc
    13981 gtttggaaca actggtttat ctttagggat taccggagac ttgtctgttg tgggcaaaat
    14041 cacgattgct acactgatgt ttattgggcg gatcggaatg ctatatacat tgatgttatt
    14101 cgtgccaaaa gaaacacgag atctaggcta tgaatatcca agcgagaaaa ttattatcgg
    14161 ttaaaatggg aatataggta agagggaata ataaaatcac agcagcttga aacaatcatt
    14221 ttgtttcaag ctgttttttt gttaaatcat gcgattttgt ccgatttcat gcactttcag
    14281 ccgtaacctt ttcttaagaa taagaaaagc aatttaacct ttttttcaac atatggtatg
    14341 attagttgaa tttattcgtc agcaaatcgt ttttggaggt tacataatgg gaatacgcag
    14401 tcattttgcg attgcagttg gaaaaacgtc gcaatgggca ttaaaaactt tttttaaagg
    14461 cggatcaagt ttaccaggta aactagcgct gaaagtcgat ccacatatac tagatagttt
    14521 ggcaaaagac taccaagtcg tcgttgtcac cggaacaaac ggtaaaacat tgactaccgc
    14581 tttaacagtc aatattttgc gccagcaatt cgatgaagtt ctaaccaacc ctactggtgc
    14641 gaatatggta cagggaattg tatcgacttt cttgcaggca aaaccaaaaa aaggacagaa
    14701 aaagtttgct gtcctagaaa tagatgaagc aagtttgagc aaagtcacag aatatatgaa
    14761 accagaactg ttcttattta caaatatttt ccgtgatcaa atggatcggt atggagaaat
    14821 ctatacgacc tataaaatga ttgtagacgg tgccgcaaag tcaccaaatg caacgattat
    14881 cagtaacggt gattcaccaa tcttcaattc tatcgaaaca gtcaatccta gaaaatatta
    14941 tggctttgac catgaagaag atcgtgaaca aatggctcat tacaatacag acggtgtctt
    15001 atgtccaaat tgtcatcaca tcttgcatta taaaatgatc acttatgcaa acttagggaa
    15061 atattactgt ccaaattgtg acttcaaacg tcctgaattg gattatcgat taactgatat
    15121 ggtacggatg gacaatgtgt cagcagattt cgtgatcgat ggtcatgaat acggaatcga
    15181 agttggcggt atgtataatg tctacaatgc tttagcagct actgcagtgg cagagtactt
    15241 caatgtcgat cctgataaaa tcaaacaagg tttaggatat gacgagaaag tattcggccg
    15301 acaagaagtc atcaatatca atgggaaaaa atgtacatta gtacttgtca aaaatcctgt
    15361 aggtatcaat caagtaatcg atatgattgg tctagctcct tatccatttt ctttagtggc
    15421 tttattaaat gccaattacg cagacggaat cgatgtcagc tggatttggg atggtaattt
    15481 tgaaaacttt gccgatatgg atatcccagc tgttatttct ggaggagacc gtcatacaga
    15541 tatgacttta cgtctaaaag ttgccgggat tccagaaaat aaattgacag aaacaaaaga
    15601 attaccagaa gtaatcgaaa aaatcaaaga attgccaact gagcatgtct atattttagc
    15661 tacttacact gctgtattgc agcttcgtaa agaactagct gctcaaggat ttatcaaagg
    15721 aggaatgaac cgtggctaat tatgaactca atatcgccca cctttatgga aatttgatga
    15781 acacatatgg tgataacgga aaccttttga tgttgaaata tatagcagaa aaaatggggg
    15841 tttcttgcca tacggagatc gttagtatac atgaaccttt tgatgctgat aaatatgact
    15901 tggttttctt tggcggtggt caagactttg agcaattaat catttcaaaa gacatccaga
    15961 aaaagaaaga ttctttgatc aactatatcg aaaatgatgg cgtcatgtta gccatttgtg
    16021 gtggctacca attgctaggt cactactaca tgggagctaa tggagaaaag atccaaggaa
    16081 tcagtgcact ggatcactac acgttaagtc aggaaaataa ccggtttatc ggtgatatcg
    16141 ttatccataa cgaagaattc aatgaaactt attatggatt tgaaaaccac aatggtcgga
    16201 ctttcttagg tgaaggtgaa cgacctttgg ggaaagtggt acaaggtcaa ggaaataatg
    16261 gtgaagacca atctgaaggt gtcatttacc gaaatgtctt cggttcttat ttccatggac
    16321 ctatccttgc aagaaatgag aacctggcag ctcgtttgat ccgcctagct ttagaacaac
    16381 gttacggtaa agaattggat ctgccctatc ttgaaggaca aacagcctag ttattcgaaa
    16441 aaggggaatg tgacaaaaat ttcgtcacat tccccttttt cgtatcctat tttaagcgac
    16501 ttgcttggca taacggttca cgttttgctt gatattcccg attgttttcg gatcattgat
    16561 tcctgtgact tcttgaatca attgttccaa gttttcatgg atcttcattt catgcaattg
    16621 acggctttgt tcatcttcag gatcatagta attaaaaata atccccattg ttgccgtcaa
    16681 atgtgggcaa gtcaagttat gttcttgtag ttctctcact ggacgtgtaa atctttcttg
    16741 ataacctaac ttacgtaatg gtgtccgagc gacacgtgtg atcgcatcag agatattttt
    16801 gttttggaag cgatgaatga ttttttcaat atacgcatga tgctgctctg catcaaaccc
    16861 ccatttggca atcagcaaac ttcctgtttc ctgcaaaact gattttaatt gtgccacaac
    16921 taacgcatct tgcattgctt catcaatggt ctggtaacct aacaaggcac ctgtataagc
    16981 gacagtcgca tgaccagtat tgacactaaa tagtttgcgt tcgatatatg gttctagatc
    17041 atctacatat aaaacccctt ttaattggat ttttttattt tttctttggc tatcgtcaat
    17101 gacccattct ttgaaaggtt ccacttggac gaataaagga tcttcatggt gttgtaatgg
    17161 gacgattcga tcgactgctg catttggaaa gccgatatat ttgtcagtaa atgatgtttc
    17221 cggaagatat ttatacactt ctttttctag aaatgctgaa ccgccaatca tattttcaca
    17281 ggcaatgata tctaaagatg ttgtgcttcc ttccttttct ctttcttgga taccttttgc
    17341 aataaactca gcaataaaag gtaagatatt cgggccaatg gcggtagtaa ctaaatctgc
    17401 ttcagcgatt tcttggatta ctttttctgg atttgtttga ttgttgattc ctttgacatt
    17461 ttttacagtg attttttctt ttgattcatc tgctaattcg atttcgtatt cttttctttc
    17521 atttaaagca tcaatgatcg tgtgattcac atctacgaaa gtaatctcaa atccattagc
    17581 tgccaaaacc tctccaataa atcctcgacc gatatttcct gcaccaaaat gtactgctct
    17641 catttttctc tctccttaag caattgctag attttccgtt acttcttctt tgctgagcgc
    17701 gtctgctagc tggacaacat tgtccatatc actgcaataa agtgcgattt gctgtacaag
    17761 ttgcaaatgc tcatctccga ctcctgcaat accgaacaaa acagtcgcaa tcttttcttc
    17821 ttcttcggta ccaaaatcta caccatctgg tacttgaacg acacagatgc cggatttttt
    17881 cacatatttt tttgcttcat ctgttccatg aggaatcgcg ataaaattcc ccatataaac
    17941 agataacatt ttgtctctat ctaccatggc atcgatatat tcctcatcaa cgcaccctgc
    18001 ttctacaagc atctgaccgc aatagcggat cgcttcttct ttggttttaa aagatcgatt
    18061 gagctcgatc atgtcgaatg gcaaactttg catgctgttt tcctccctta agctgtctgt
    18121 ttcaaatatt gtgcaatcga ttcgtatttt tgcggattga ccatgctgtt gactgataaa
    18181 tgaatggatt gaggtgcagc catctgtact ttttccgcta aactttcttt ggctacgatc
    18241 agtgaatcag gttcatcttt taaatcttgg atattgactt ttcttaacgg gatagttaaa
    18301 tggttcttgt ctaatatatc tttgagcacg gtcatcccca ttgttgctga tccggaacga
    18361 tcatcgtgga caagtagaat ctctttgatt tgtttgatgt ttgctgaaag aaactcttct
    18421 gctggctcat tctcaatttc agaaactgat gttttctgct tgctgcttaa ttcttgaatg
    18481 atttcatcat atcgaggtga attcaaaaag ttttctactg agacaaaacg cgcgctcggt
    18541 gctttttgtt ttgctctttc ttgtagttcg acctgagtaa cgatcaatgt attcgagtca
    18601 tctgtcagtt ggctgattgc ttgattggtc accgtttgag gcaagtctgc ttcctgtact
    18661 ttttttctca agatcgaagc gcccatcgca cttgatccca ttcctgcatc acaagcaaaa
    18721 atgatttttt gtacgccaga gaatgtagct ccttgaggaa taccttgggc acctttggaa
    18781 gcttgcttag ctgcctttgt ctcttgaaca gatttttcga agtcttcatt tgtatctttc
    18841 gcatccgctt tcaaaattac tgcagaaata ccaaacgaaa cagctgcggc aacaatcacg
    18901 cccaaaataa caggaaggta agaattgaac ggtgtcatcg ctagaatcgc aatgatcgac
    18961 cctggagaag ctggcgctct tagtccagca tccatcaatt ggaaaatgaa acttcctgac
    19021 attcctccgc ctataactga taagaataat aacggtttca tcataacgta aggaaaatag
    19081 atttcatgga tcccgcctaa aaagtgaatg atcatggctc ctggtgctga agattttgct
    19141 gctcctttgc caaacaaagt aaaagcaagt aatacgccaa gtcctggacc aggatttgct
    19201 tcaagcaaga atagaataga ttttccagcc tctgctactt gttcagttcc caatggtgtc
    19261 aaaatcccat gattgattgc attattcaaa aataagattt ttgctggttc aataaaaata
    19321 cttgtcaatg gaatcaaatg ggcagataaa atgatctcta caccttttgc cattgcttga
    19381 gtcaatgcat cgacaactgg accaatcgca gaaaaaccta ataacgctaa tccaaaaccg
    19441 ataagacctg ctgaaaaatt attgactagc atttcaaaac cggatttgat ccgtttttgg
    19501 aatagctgat caaatttctt gatggcatat ccaccaagag gtcccatgat catcgcgcct
    19561 agcatcattg gaatatctgt tccgacaatc acacccatcg ttgcaattgc cccaacaact
    19621 gatccgcggt caccagcaat tacttttccg ccagtataac cgatcaataa aggaatcaga
    19681 taagttagca ttgggcttac catggtagcg aaagcctcat ttggtaaata accatctggg
    19741 ataaatagag ccgttaatac accccaggca atcagtgctc caatatttgg catgaccatg
    19801 cttgataaag tactgcctaa tttttgtacc ctcgctttta gcgaaatgtt gtttttggga
    19861 atcgtccgtt ccattttgtc tctccctctc tccttttgat actctaattg tatccgctta
    19921 ctaaaaagag aaatagtcgt actttggcac gaaactttgt gccaaaggtt tgagtatgtc
    19981 agcaaaataa aaagatttcg gtatcagaaa acttcaattt taaataaaac tgttatttta
    20041 atttttttat aatatgcaaa caataacttt tgttagcatt taatttagat gttttccatt
    20101 ctattatgtt attaaatttt atattggagg cctatctatg acattactga tcaaaaacat
    20161 gaacatgaaa cgtggtaaaa aacgtgtgat tcgacaattg aatcttttag tagaatcagg
    20221 agaagtagct gctttgattg ccccgaacgg atttggcaaa acaactttgc tgaatggcat
    20281 tgcccaactg ctgccaacaa cttatgatca attgtccctt cacaacatct ctcctactgc
    20341 ttatacagat tatcgccggt ctttcttttt tctagaatca acgaatcagt tatccgacta
    20401 tctatcggct gttgattatt tgactttgat caaatactat tggcagagca aagtagaatg
    20461 ccaaactgtt ttccaacgat tgaagatgac tgagtacctt actttgccga ttggaaaaat
    20521 gtctctagga atgaaacaac atgtcttgct tgctgcttac ttgatcagtg attcatctgt
    20581 catgttattc gatgaaccgt tgaacggatt agacccagga agtattgata tcttgaatag
    20641 cctcatcaga caatggcaga aagaggggaa gacgattctt ctttcttccc acgctatcgc
    20701 gaatatccaa tcgatttgtt ctaaagcatg ttttctaaaa aatggagaaa tcgtcacaga
    20761 atcctcaaat atggaagaga ttttagctat ttatcgtaaa ttatatgagg tgtaactatg
    20821 tctttactta catttgaacg caatctctta ctaaaaaaca aagtaaactt tttatttcct
    20881 ctattgttgg tcgtgctctt tgcattccca cttttttttg atcataaact tgcttataca
    20941 gaattcgatg aactgaatca taactatgaa gagatgcaac gtttgattga aacgttgaaa
    21001 gaagatgaga atgagaaaga atttgtggaa agtttagaga aaagcaacaa actgatcgaa
    21061 gcaatcttac atgcaaaaaa cacagggaat gtccagcaaa tggtcgaagc aacttatcat
    21121 tatgaaaaag atatactgga tcgactgatt tctggtcaaa gacaaggaat tccaatcatt
    21181 gaacaacaaa aacgtgtcga gttgctccgt tatatgaaag aacatcagat ccaaagatat
    21241 tctatttttg acttgccagc gcatctctct ttagcaaatt actacgaaaa tattttttca
    21301 ggaatgattt ctagtttttt gattttatgt ataaccgcct tgtttctttc ttccatcatc
    21361 tcttatgaaa aaagaaagca ggtaatctct ctagtcaatc tattaccgga tagtatggta
    21421 aagaaacata gcatccgttt taccatttat tatggtgcag ctatgctaag tctcgtgatg
    21481 ccatttttga tcgtctctat actagtaata atcaaaaatg gtttgggaga cttccgttat
    21541 cctgtaggaa cgatcattgg tcaggagata cgaatcctgc cgatgtatga gtatcttttc
    21601 cagagctttc tgttcctgtt gttgtgggtg ctctttttaa gtacaatcag ttttcttcta
    21661 tctgctttat ttgaacattc tctagtcaat cttttaggta cacttctttg tcttttctta
    21721 gcagagtatc gcttgttttc ttcgattgga tggatagaat caatcagtca ttacttgcca
    21781 acttcttatg ttgattttca aaatgtgatc ataggaggag atattttctc gccactagct
    21841 tcagaacagg ttactttcat gaatggtatc ttgacacttg gtatatggag tatcgtttta
    21901 ttattcatag gtatgggcac tatctatata aaaaagagct attaaaacac aaaaataata
    21961 ggggcggtga caaaaacctt gtcaccgtcc cctatttcaa ataaacggtt ttctagaata
    22021 aatagttttc aaaagcttgc atccgcagta ataagaacct cccaacaaaa atagctcctc
    22081 ttatttttat cttggttgaa gttattactt gatgcaaggc agcttttttt actgtgaaca
    22141 ttggttatat atgtccatgt tcaaaaatca tttggacttc ttcaatggtt tccgcatcac
    22201 tgagcgccat tacttggtcg atgtctgaac agaaaaaggc taaatcttgg agcaccgcta
    22261 actgatgttc tttttttaaa gccactacaa ataagagtgt cgcgattttt ggttcctctt
    22321 ttgttccaaa gtccacacca tcaggcactt ggactaaata gattccttct tctactactt
    22381 cttcatcttt gtcagtccca tgtggcaagg ctacgaaatt cccgatatag acactcgctt
    22441 tttcttgtct ttcgatcatt gattggatat atgccggttt gattccccct tgttgggtca
    22501 tccactggcc gatctcggtc aacgcttctt tccaattttg ataggattga tcgagcaata
    22561 ttttttctgc tggtagctgt atcatctttt caccttcatt tctttatttc ttcaatcagg
    22621 attttcgcta acaactgata aacgatttct ttatttcctg agcggaagat ctcggtgttc
    22681 aagtcattca tgatgattgc tccactgatc tttcctaaaa gaatcccgtc tttttcttgg
    22741 attggcgaag gcgccagcat aactaacata cgggacaaat cgactggctg tctatccatc
    22801 ccttcaatgg tcaacggatg tttcaaattg aaaatacaaa agatcggttt agaaatcaat
    22861 gagctggatg tatggaataa ccccatattt gtatgaggaa taccgatagg tgcttgttca
    22921 tatcgcttaa gcagccggcg atgaaccacc tcttcatccg ttactagtcc ttcaggaaaa
    22981 taaggaaata agctgcgaat cgtttcggaa atcgtctcag gattatccag ttcttggata
    23041 aaaaaacgac cgagtaattg attgatttga tccatgaaat gcattgtttc ctcataagta
    23101 tcttctatca tccactcgtt attgctgtta tacgtaattt tttgcctttt tttcttctca
    23161 atatgcgaaa aagcttcttt tagctgcttg acttcatctt ctagcagtag cggggagact
    23221 aaaatatatt ttccaccgta tcctggtaaa atagatgtag aaataacaac atcatattct
    23281 tcttgtaaat cgattttttt taaatcagca atccgataga aatgaatttg ttggatgaag
    23341 ggaaaatacc ttttcaatcg tagttccaac atgctagtag aagctaatcc acttggcgaa
    23401 aatcctgcaa tactgatatt gctttctttc ggacttcgtt ccaatgaatt cgcaaaatga
    23461 aggaccatat aagcgatctc ttcttcggat aatttttttt cttgaaaaat ccgctctact
    23521 gctttacgga ttgcattcgc aacttcctcg tattgaacca tgattttttc taaaatcgga
    23581 ttggtcagtg tctcttcttg taaaacggct cttgaaaaca caccagataa atgagtcaac
    23641 agcattttgt acaaagtttc atcttcataa aaatggacat tcgtctcttc acttacttct
    23701 tgaatcagtt gttttactaa ataagcaagc tgtgtatcga acgtctcttc aaagaaattc
    23761 tgatcaaatg agttcgtgaa gtcatttaat aatcctgcaa aaaagacgat ctcatttact
    23821 ggataaagtt gttttgtctc agaagcgatc cgtgaaaaca atcgtttagc tgtctgtagt
    23881 aattctttgt ttgccagact attatatgtc tcatttgtca gtaaatgatt tttcgtcaca
    23941 cgctccatcg tcaatgtcag catcaaaacc aaatgttcaa gctttctatc acttagattg
    24001 gtcttttttt ggatcaattc ttccccgatt actttgcgtg caagagatag atgttgcgga
    24061 tcaacgaatc gaaaaaaata attcagtgat gcatcgaaag aaatactatg aaaaagttca
    24121 tattcattga tttctaattc taatacattc gctgtcagaa gccggcggta tttttccggt
    24181 ccttcgatct cgtatccctg gttccgaatg atttccaaag gcagttggcg aatactttct
    24241 tccaactgtt tgatatctgc ataaaaagtg gtattactga tgcgatatga ttccatgaaa
    24301 tgagcaagac tcaacggctc tgtcgtcagt aacaactcgg ctaaaatcgc gtgttgtcgt
    24361 ttagcagtgg ataagtcatt acttttccca tctgtatttt cttttaattg ctctaatact
    24421 tcttctgatg cctcaatgac aaatgagcct ctagctgctt ttttcaaaga agcatttacc
    24481 gatttcaatg agacttcaag attatctaat tcgcggtaaa tagtccgttt actgaccttc
    24541 aataaattca tcattttttg gatagataca ggtgacgggc tactgagaaa ttctgctaat
    24601 aattgttttt ctctgggcga taaatacatc ttatatttct cccttcacga gtttttcgta
    24661 ttcattcgta gctacccaat gttcaactag aactagccga gggatctttt cttctagctg
    24721 ttcagttaca gctagttctc tttgtgcgat ataaatcgtt tcttcttgga ttttgatttt
    24781 ttctattgat tcttttgaga taacaaactg ttttttcgct ttttttgcta attgacttaa
    24841 aatcgtcatg cccatagttt gtgagccgcg tctgttgttt ttatacaaaa atacaatatt
    24901 agcaggcgac gttttttgtt ctgtgatatc tgctatctct tttttttctt cccgctttgg
    24961 aaattggatc gcttctttta ttcgtgacag caagatatct gtttccaaaa agtgatccag
    25021 tacatagatt tgactttgag gtactttttc tcttgcaaga cccgctagtt catgttgcac
    25081 gataaccagc tgatttactt ggttattcaa ttcatagata gagctgtatc cgacagaaag
    25141 atcgattttt tctgctgcta atttcttttt cagcagggcg gctcccatag cacttgatcc
    25201 catacctgag tcgcaagcaa aaatgacgga atgaatgata gatggttcca agatttctcc
    25261 cctttctttg accgttttgt tctctttttc tttatttggt aatttcaata gtatcgctgc
    25321 acaaatgaag gtcacaaatg tactgattgc tatccctata cttaccccag ctagttgtgg
    25381 ataaggcgca ttggcaaaaa tcacgagtag tgacccaggc gatacaggtg cagctaatcc
    25441 cgtatctaac cattgaaata tcaacgtgcc gcttgcccca ccagccatta cagctaaaaa
    25501 tagatacggt cgcagcaaga caaatggaaa atagatttcg tgaatcccgc caatcgcatg
    25561 gatcatcaat gctccactcg catttacttt ttcctgtttt tctgcaaaac aaaggataga
    25621 aagtaagatc cctaaaccag gtccaggatt tgcctccatc aaaaataagg aagagtaccc
    25681 gactgtttgt gagaattcga ttcctaaagg tgtcaataca ccatgattga ttgcattatt
    25741 gaaaaatagt acttttagag gttctaagat cagactgaca agtggtaaca gattcattgt
    25801 gactagccaa gacacaacgg aggcgagcaa caagtgaaca ctatttaaaa ctggttcaat
    25861 aaaaacgatt ccaattatac aaataacgct accagtcagt cctactagta ggttgctgac
    25921 aagcatttca tatccagcct tgaatttcac atggaatcta gaaacaaatt gttcatatac
    25981 gatgccggca agcggcccga taatcatcgc tcctacgatt tgtggagcat tggaacgagc
    26041 gatcaaccct aaagaagcaa ttgcaccggc gactgcccct ttttgtcccc ctaccgattt
    26101 cccgcctgaa taactgatca agatgggtaa aagcagctgg atcatccaat ggcatacctc
    26161 ttctaatgtg gtgtgcaaat cgccagacac accttttgtt gctaatgaca agattcccca
    26221 agcgataaac agacttagat ttggcatgat catttcattt aagtgatgtc cagcttttct
    26281 cagttgtttc atagacacgc gactgctgtt gtttgctttc catttgtatt ccatcttttt
    26341 caccagactt ctatttattg atggacggat tttatgtaga aaaagcagca aacgaaaaac
    26401 cgtctgctgc tttcagctat ctgggaacta tttgcctggt tcagacgcaa tgattgctag
    26461 ttcaccttgg accgcccact cagaaacgcc atttggaata atacagcttg ttcctttttc
    26521 taatggatat gtttttccgt caacgacaag gtctcctgct ccttcgatta ccgtcatcaa
    26581 tgtataaggg gcttgttttt tgaaagatgt gattccttta atatcccact catatacatt
    26641 gaaaaactca gtttctaaat aagtgacgat cgatgaattc ccttttcgga cttctttgat
    26701 ttgtaattct ggtgttttcg cagggacagt tgttacatcg atcgattgtt ggatatgcag
    26761 ctctcttgtt tgtccttgcg catcttttct gtcgtagtca tacacgcggt aagtcgtgtc
    26821 agaactttgc tgtgtttcta aaatcatgat tcctttacca atagcatgga tcgttccgct
    26881 tggtacataa aagaaatcac cttttttaac tggtactttc ttcaaaaggt catcccagcg
    26941 cccatctttg atcatctcag ctagttcttc tcttgtcttt gcatgatgac catagatgat
    27001 ttctgcacca ggttcagcat cgatgatata ccagcattca gtttttccta attcaccttc
    27061 atgtttcatc ccataggcat catctggatg tacttgtaca gatagatcat cttctgcgtc
    27121 taagatcttg atcaataatg gaaacactgg ttctgaagga tggccaaaca attccttgtg
    27181 ttctgaccat agttcatcta atcttttccc tttgaattcg ccattttcta ctacactgac
    27241 accatgagga tgcgcactga tcgcccaatc ttctccgatt ttatcgttag ggatatcaaa
    27301 accaaagact gaacgtaaac gacttcctcc ccaaattttt tcttgaaaaa ctggttttaa
    27361 aaacataggt tcctgcataa ttcaaactcc tctcattttt catagctatt atagcattat
    27421 ttcggtaata ccgctttctc tttcaaaaag ttttcaacaa tttcatctcg acatatgaga
    27481 tactccgcac gatgatcgac aggatgtacg cgatttgaca aaaatatcaa cgcttcttgt
    27541 gtttttatat caatcaagaa aaacgtacct gtataaccag tatgaaaaag aatcgcatga
    27601 ccgtcttcta catccttttt taagtcccat cctaaagaac gatagccatt ttttttcggt
    27661 gtttgatctt gcagtaaaga atcaatcgtc tcttcttcta aaaaacgacc attacggatt
    27721 tttcccttgt tcaaatacat ttggacaaaa gtaatgctgt ctttcaaatc aataaagaga
    27781 cccgcgttac ctgcatgttc agccaaaact cttgctttcg gatcatgagt ttgtccacgc
    27841 aaaacctcac ccgaaggcag ttgttctgtg ggaacgattt ttttggaagg aaaaggaaga
    27901 aataagctgt tcatcattcc taatggttcc aatacttgtt cttgaaagat ttccactgca
    27961 gtttcttggt aaatttcctc tagcatcaac ccaagaagaa tcgtaccggc atctgtatat
    28021 tttacttctt ttcctagttg ctcacctggt tctagcttca aataagcggc gatcaattct
    28081 tcctgagata gcttatctct gtttggtatc catgttttga tatcagaagt atgtgtcagt
    28141 aaatgacgca aagtgattgt ctgattttca aattcaggaa gatatttatg aagcggttga
    28201 tcgagttcta tctttccatc ttctaataga cgtaaaacta atgaagtggt tgcaacgact
    28261 ttcgtcagtg aggctgcatc aaacaacaga tcttctgtca tcagctgctt ttcaggaacg
    28321 acttgggcaa agccggctgt aaatgtttct acttgatttt cttttatgaa ggcgactgct
    28381 acacctggat aaaatccttc ttccatcttt ttcaaaataa gctgtttcgt gtgtggatac
    28441 atctttttct cctgcttttc tatcagattc ttacagtcca agcttaaagc ttaatctatg
    28501 aggaatcaac cttttttaca caaaaaaact gcgcgaaaaa ccgcgcagtt tcccccggta
    28561 tactattttc tctttctatc tctagaataa gcgatagtat gaccgttcat aaaatgacga
    28621 gacatgcgac aatcgttaag aaagaaaaag ttaagcgtcg catcgaatgt cagttttatt
    28681 cttttctttt ggctcactga cgaaccacca ttcgattcct atagcatcat agattgtgag
    28741 tactgtgtgc tttttgtcta agaagaaatc aactgatagt tgctgcaagt gagcaatcag
    28801 tccttccatg ctttcagtat cgatcatgaa tttcagaaaa tcaagtccaa tcacttcatc
    28861 tgtaggcaaa tcaattgcgc ctccgctagc ttctgtcaaa ccaacatgga aatctccttc
    28921 attcaataca tggatgccct cttcttcatt atgtaccttc aaaccaagta catgattaag
    28981 aaattggcgt tcttttgtta agttagtaac atttaaatgg attttttcaa aataagaacc
    29041 tgttgatagt ttactgaatt tttcttcaga ttttcttaat aatgcttctt gatccattgt
    29101 ttctggaatt tctctttggt cgttcttatt ctcttcatgg taaatttcca attcattctc
    29161 ttctgggtct acgagcaaaa tccccatacg tccatgatct tctaaagctt ctttgattgg
    29221 ataattcatt ttacggatac gacaaaggat atctgccatt tcttctgtgc tagggataat
    29281 caatgaaaaa cggttcagct ttttgatcac ccccatgtga tcattcgctc taggactttc
    29341 ttccagcagc aacatttcac ttccagcttc ttctgttcct aaaatggcta attcgttttc
    29401 ttctcttttc aaatcaaagc caacaacgtt cttgtaaaaa tcaatcattt tatctcggtc
    29461 tttcacccga atggctacac tttttaaata ggtggcagcc cccaattgaa aatctttcat
    29521 atttcttgct cccctctacg tcaagcttta ttttaacgat ggattcttct tctttcaagc
    29581 aataactttg attttgttaa aaaaaatgtg agaaaattcg cattttgctt gttttttgta
    29641 aattaatgag taaaaactct tataaaaaga ggttgtgaaa aagctgttct gcatcaagta
    29701 ataagaacag ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg
    29761 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaaagtcg cttagtttcg
    29821 agtagtaaga aaaattttgt gaaaatggct ttctacattt ttgcataaat ttggcttaat
    29881 accgagaaac tggcttttga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc
    29941 cgagtcacaa ggagcaattt caaaaaacag cttctcaggt ttgttgacta ttgtgacttg
    30001 tgaccgagga gttggcagga gaacaccgtt taaggaagag gttgtaaaat cagcggtttg
    30061 acctgagtat tagagataca aaacggaaaa atagcttctc atattttgcc gttttttgag
    30121 ctaataccga aggacagctg attgaacacc gtttattcgg aaaaagggac tgtgacacga
    30181 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
    30241 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
    30301 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
    30361 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
    30421 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
    30481 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
    30541 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
    30601 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
    30661 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
    30721 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
    30781 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
    30841 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
    30901 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
    30961 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
    31021 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
    31081 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
    31141 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
    31201 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
    31261 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
    31321 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
    31381 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
    31441 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
    31501 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
    31561 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
    31621 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
    31681 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
    31741 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
    31801 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
    31861 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
    31921 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
    31981 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
    32041 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
    32101 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
    32161 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
    32221 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
    32281 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
    32341 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
    32401 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
    32461 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
    32521 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
    32581 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
    32641 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
    32701 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
    32761 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
    32821 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
    32881 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
    32941 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
    33001 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
    33061 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
    33121 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
    33181 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
    33241 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
    33301 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
    33361 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
    33421 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
    33481 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
    33541 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
    33601 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
    33661 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
    33721 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
    33781 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
    33841 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
    33901 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
    33961 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
    34021 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
    34081 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
    34141 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
    34201 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
    34261 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
    34321 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
    34381 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
    34441 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
    34501 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
    34561 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
    34621 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
    34681 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
    34741 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
    34801 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
    34861 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
    34921 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
    34981 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
    35041 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
    35101 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
    35161 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
    35221 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
    35281 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
    35341 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
    35401 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
    35461 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
    35521 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
    35581 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
    35641 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
    35701 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
    35761 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
    35821 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
    35881 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
    35941 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
    36001 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
    36061 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
    36121 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
    36181 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
    36241 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
    36301 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
    36361 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
    36421 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
    36481 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
    36541 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
    36601 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
    36661 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
    36721 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
    36781 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
    36841 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
    36901 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
    36961 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
    37021 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
    37081 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
    37141 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
    37201 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
    37261 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
    37321 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
    37381 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
    37441 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
    37501 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
    37561 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
    37621 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
    37681 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
    37741 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
    37801 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
    37861 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
    37921 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
    37981 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
    38041 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
    38101 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
    38161 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
    38221 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
    38281 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
    38341 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
    38401 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
    38461 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
    38521 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
    38581 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
    38641 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
    38701 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
    38761 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
    38821 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
    38881 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
    38941 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
    39001 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
    39061 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
    39121 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
    39181 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
    39241 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
    39301 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
    39361 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
    39421 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
    39481 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
    39541 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
    39601 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
    39661 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
    39721 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
    39781 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
    39841 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
    39901 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
    39961 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
    40021 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
    40081 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
    40141 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    40201 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    40261 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    40321 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    40381 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    40441 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    40501 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    40561 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    40621 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    40681 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    40741 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    40801 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    40861 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    40921 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    40981 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    41041 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    41101 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    41161 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    41221 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    41281 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    41341 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    41401 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    41461 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    41521 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    41581 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    41641 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    41701 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    41761 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    41821 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    41881 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc ctagatgagg caacataagt
    41941 atacggtcat tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt
    42001 tgtttttcca atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg
    42061 ataagtacag catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta
    42121 tcttctgtca acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa
    42181 tctgcttgcc gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc
    42241 cctaagctat agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg
    42301 atggttttgc cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct
    42361 aaaatattag gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa
    42421 gataagagca aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta
    42481 ttcatgacga tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca
    42541 ccagctcttg aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta
    42601 cgaataacta ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac
    42661 ttttctatct cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct
    42721 ccttactaac aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc
    42781 tagaagcaaa aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca
    42841 agttactgcc ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa
    42901 atggtgttcc gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa
    42961 atttcgtcaa tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat
    43021 ctaataacca ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt
    43081 agatttagcg agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct
    43141 tcatggaaat tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca
    43201 gtcgttggtt caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct
    43261 gccatcgata cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct
    43321 tctctatctc ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc
    43381 ggatctactt ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa
    43441 gtataatctt ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca
    43501 atcgctgcag ttttccagtc gccttcccca gcaaaaccgt atccttctgc catcaaacgt
    43561 tgtacagcta gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa
    43621 gcgttatagc ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact
    43681 tttacatgtt cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc
    43741 ttgtactctt caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca
    43801 agatctccga taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg
    43861 tctccttctg tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca
    43921 aaactttcat tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca
    43981 ggtcgtttcc agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac
    44041 ccatattcgc ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt
    44101 ttccaaggaa tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc
    44161 tttgtcccgc ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct
    44221 gctacttctt ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact
    44281 gctaattctt gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc
    44341 atttcttgtg catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat
    44401 cctacaacaa accaaaattc tttttctcca atattcaaca taattagcca ctccttttat
    44461 ttgttttgtc cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc
    44521 cgttgaggca gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat
    44581 acttcatcta gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat
    44641 ggaccgtgtc cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg
    44701 aatgtttcta tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt
    44761 gtcaaaaatc gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc
    44821 gcaggaacat ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg
    44881 attcctccga tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag
    44941 ggattgagtt taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct
    45001 gtcatctctt cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca
    45061 aaagcgctca catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt
    45121 gcttggtata cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa
    45181 ttgatgaatc gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg
    45241 atagcctgct atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac
    45301 gtcttctgcc aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa
    45361 taacgcgatt ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc
    45421 catagcagat gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc
    45481 cactaaacga tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc
    45541 accaaaagca ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg
    45601 tgtacgtaca aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata
    45661 accataagaa agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac
    45721 cgtgaataat ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat
    45781 actgacccaa gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc
    45841 tcctgaaggt gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa
    45901 tacgatcata gcgaaggcgg aagtaccagc cgaaatgttc cctgtccctt tggctacact
    45961 gttcgtagcg accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg
    46021 tctcaaattt cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc
    46081 ttctcctgct actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt
    46141 ttcgattaac tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc
    46201 gataggaaac atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca
    46261 atggacataa cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt
    46321 caaaatagat tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt
    46381 ttccgttaat attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac
    46441 tagtaactgt tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat
    46501 cgacccgatt gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg
    46561 gtaactgacg cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc
    46621 tagtttgttt tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa
    46681 tacagctttg atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac
    46741 atctaacata ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca
    46801 aacctgatca aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt
    46861 acatgtcaag cagttattta caagaataca ataaaaaaca cttgttttgt acgtacataa
    46921 gttataaaat gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga
    46981 gagaatactt atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc
    47041 atttggcggc attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt
    47101 acagcatgat tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt
    47161 catgttaggt gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg
    47221 acgaaaaatg attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc
    47281 tgcccctcac aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt
    47341 tggcgctgca tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag
    47401 gggaagattg tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt
    47461 tgcagattat ttgctaaaag ggttgcctga aacgatggct tggcgcgtga tgttagggct
    47521 cgctgctgtc cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg
    47581 ttttcttatg caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac
    47641 accaaaagaa gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac
    47701 aaccggaact tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg
    47761 tattggtgta gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc
    47821 gctgatcgta gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat
    47881 ccaaggaatc attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa
    47941 tcggcgaact ttattgaaaa taggcggtag cgtgatggga ctttctttca tcctaccagc
    48001 tgttttagga actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg
    48061 tatttatgtc gcattttatt cttgcacatg ggctccactg acatgggtaa tcgtaggcga
    48121 aatattccca ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat
    48181 tggttctttc ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat
    48241 cgttttcggc atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt
    48301 accagaaact cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta
    48361 tcctaaacgt atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca
    48421 agaaataaga aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc
    48481 tcatgtttga tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag
    48541 aggtggtgaa agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac
    48601 gagtaaccgt aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt
    48661 cactgtttcc cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt
    48721 ttcaatcgtt gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt
    48781 caatgttgtc aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat
    48841 cgataggtca tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc
    48901 tatttgatcg ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac
    48961 tgttttttca gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa
    49021 aaaacgcaca ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg
    49081 aagatcgtcg atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc
    49141 tgctgccata aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc
    49201 ataagcagca ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc
    49261 taagtttggg ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata
    49321 agcaatcatt ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa
    49381 aagcaatgaa tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa
    49441 aatataatca gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt
    49501 tttttctttc ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata
    49561 gccctcattt accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat
    49621 ttcttgcagc ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat
    49681 cttttgtttt atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta
    49741 tttgtacgta catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt
    49801 cttgtgctca taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc
    49861 acgtaaagca ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt
    49921 tcttctctgg tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa
    49981 acgagttgtg accaggacca aattgcttat ttttttcaga agaaacgaat actggctccg
    50041 ataatttatg ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc
    50101 ccatggcata gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc
    50161 gagtaaggac tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag
    50221 gtatagaaag gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat
    50281 aaagattaga attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc
    50341 cttgagaaga gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc
    50401 ccttttctat ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc
    50461 ggtgatgatg agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc
    50521 attttccatt gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt
    50581 catgagcctg ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc
    50641 ttcttaattc aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg
    50701 tatgtttata tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct
    50761 tcatcataac aaatttcctc acattttctt tattctaatt acattccaag agtatttttc
    50821 tatccctaca tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat
    50881 tttttctggt tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg
    50941 ctctccttga tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt
    51001 gcgattgatc aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata
    51061 aggaacattt tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac
    51121 ttgaccttta gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc
    51181 ttgtccttta gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact
    51241 aatctttacg cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt
    51301 atcttccata gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct
    51361 tttttccggt tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact
    51421 tctcaaatat ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa
    51481 tcgataagct tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt
    51541 caagtcttct tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc
    51601 ataagcttct tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga
    51661 gcttccaact aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc
    51721 atttgctaac ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag
    51781 ctcattccct aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct
    51841 taaatcactc cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc
    51901 atcaattcct tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat
    51961 ccatttaaaa aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc
    52021 aatttttttt ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt
    52081 aaagtttcct ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac
    52141 atcttgtcta aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata
    52201 gacgacactt cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac
    52261 cgtaaaatcc tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc
    52321 atttcctata gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat
    52381 aaaaggaggg aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat
    52441 atttctcaaa aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt
    52501 gatatctttg ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag
    52561 taaaactaca tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac
    52621 aagaaaataa ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt
    52681 tattccttga aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat
    52741 tacataaaaa acagcacatg tagcaggcac gatagtaata acaggcaaac acacgattac
    52801 ccagagaata cctaataaga tgtaatcaga aaccacaatc aactgtccat aaagtttagt
    52861 ctcaactatt ttcatcttcc tcactctttc attccagaca tagctgttcc ttgaacaaaa
    52921 tatttctgtg caaaaatata aaccactagt aatggcagca ttgaaataac cgttccagcc
    52981 attacaggtc cataaaaaga aatgttctgc cctgtaaata aagctaaccc tgctggtaaa
    53041 gttctcattt ttgaactact tgtcaatatc aatggataaa gcaagtcatt ccaactattc
    53101 atcagcgtga aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa catgattttc
    53161 aaaaaaatat taaactcact taatccatca atgcgtgctg cctcttccaa agaatctggt
    53221 aaagtaataa aaaaggaacg catcatgaag ataccaaatg cagttgtcat acgaggaatg
    53281 atcaaacctg cataagtatc aagcatgcca agcaaattga cttcaataaa tagaggaatc
    53341 atgaagactt ggaatgggat catcattgtc agcaaaacga aatagaacaa aactgatttt
    53401 cctttgaaac gcatacgagc aaatgcatac cccgcaagag aatcaaagaa tacagaagtg
    53461 atcactgtcc ctccagcaaa aatcactgta tttttgatgt aatctaataa agggatcgtt
    53521 gtccaaacct taatatagtt atctagggta tagctatctg caaatattct aggcggaaat
    53581 gagataatgt ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa aggaaacgca
    53641 ataaccaaca atataactag catactaatc gttcgaatga tttttttacc actaactgct
    53701 tttcgactat tcacagtaac tgtaatcgag ctttctggtg ccatacaggt ttccctccta
    53761 tttcatttgt ttttctttcc gtgacatgta cacattagtg gagatagcta tagttgcaac
    53821 aatgataaac aaaaccgaag acagtgctga tgcatagccc aattcataag gtgctgtaaa
    53881 tcctcgactg taaatatatt gaacagctgt ttctgtctta aattgtgggc caccagcggt
    53941 tgcaacatat acttggtcaa acacttgcat cgaaccaatc agattcgtta aaatacaaaa
    54001 acctaaggag ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc
    54061 accatccatt tgagcagatt catacaaaga gggagagatt ccctgaatag cagtcaagag
    54121 aatcgtcata gtaacaccaa aattcttcca aacagtcatg atggctaccg tcggcatagc
    54181 aagggttgga tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa acaagtaagg
    54241 aatcgcccct acattcggat caagcaacat agaaaaaata atcgcaattg acgtcaatga
    54301 acaaataact ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg tatttttaga
    54361 taatgctact gctgctaata aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa
    54421 atataaagaa tgaatcaacg aattaactgc tcgttcatca ctaaatatac gtgtgaaatt
    54481 ttcaaaactt acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc
    54541 aaatgttcct aaaagcggta tgacagtaaa taataataaa actaaaaaag atggagttaa
    54601 aaacaaatac gcagcacgat caggacgatt ccaatatttg gtgaatccgt tcatatagtc
    54661 cacctcatct ttcattttga taaaatatac aatcttggtt taaacgaatc tgaataagat
    54721 atccgaacaa tcgctgtgat ttttgcaata acacctatct gcaaatacta ggtcgatcat
    54781 tcagatatct gtatcttgcc aacagtctat cctgtataaa gttctgtact atttttgcag
    54841 taattgattg atggcatcat ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa
    54901 taatttttca atcagtggat tgatgatatc gttattgatc gcagagattt gggtaaaacc
    54961 tggtaaatat ggctctgcat agtccatttg tttcgataat tcagatacaa taggatcatt
    55021 ttttacttct tcatcttttg ctactgaatc caagtaggca ggaaaaccat tttcgatact
    55081 ccatttttta ccaatttcag ttgtattcca atatttcaaa aactcataga cagcttcttt
    55141 tttattatta tctgtacttg ccggaatggc atatcctacg ccatctaaaa tagctgtttt
    55201 tttgtctgaa tcactccctt gtggtaaggt agtcacatta taatcaatct cgtttgcttt
    55261 caatccgtta tttaaccatg gcccattgat ttcaatggct agttgaccag cattcattaa
    55321 attgtccatt tcggcgccag aaatcgattc tggaccagtc ttgtctacat ggatcatttt
    55381 ttggatcgct tttaacgttt taaggttctc aggagacgca aattcagact tagtataatc
    55441 atcgttcact aatttaccgc catttgctaa catccagtta tataacggtg cagttccatc
    55501 cttattgaag acaaaacctg atacattttt actactatca gttaattttg gtgccatttc
    55561 tgtcaattct tcccaagtag taggtggttg ttcagtatct aatccagctt gtttaaacaa
    55621 atcattattc caatacatat acatgccttg tacctgcatg gggacaaaat actgtttacc
    55681 ttctacttca cctaattcaa tagccgtttc tttaaaatca gatttatcta ctccatcata
    55741 ttcccagaaa tcgtctaatg gttgtaccgc tccgttttct acatactgtg caaaatctgg
    55801 ataatttaat gcaataaagt caggtgcttt tttagaagaa atcgctgtag gaagtttttc
    55861 attcaaattg gcccaagtca tgatatccat ttcaattttg atgtttttat catttgtttt
    55921 attaaattca ttaacgattt cttgcaatat ttcaccatca gatgcagtaa acccattcca
    55981 aaaagtcaaa gtcacttctt tatcagaatc agattctgct gaacttccgc caccgcaagc
    56041 tcctaaaata cctgttgcag ctaccaacaa agagccaatt aaccattttt tacctttcat
    56101 tttttgttcc tccttcatct gataaacgac atctgaccac attccaagat agcggtttca
    56161 ataaacaatt gattttccca tcttcagctt ttacatttgt tgaatgactt ggttttactt
    56221 gttcactttt aggaagatta gttgcttttg tatcaaatcc actcatttca gaaaaatcaa
    56281 tcactccctc taatgataat tcagacaagt gaacagttag attcatttct tcttctccac
    56341 ggttaactgc aaatatgaca agttctttgt catttattac ggctatactt tctaaatacg
    56401 gcacttcttc aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg cttaacacct
    56461 ctccttgtcc atacacagca gcttgcataa atggataata gatcgtctgt ctccaagctt
    56521 ctccgtttgt ttctgtcctg attggtgcaa taacatttac caattgagca agacaagcaa
    56581 tctttactct atctgcatgt ttcaataaag tgattaatag acagcctaat agtaatgcat
    56641 cttcaaaatt atagttatct tctagtaaag gaggagcaat ctgccaaggc gctgcttgct
    56701 catcttgttc cgctgaatga taccaaatat tccattcatc aaaggacagg ttgatctgct
    56761 tcttactatg tttcttcgcc ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc
    56821 gatccatgtc taaggaacgt gcaagataat gagaaagatc atttttttgg ttgccataat
    56881 attgatgcag agacaaataa tctatttgat cgtacgcttg atcaagaact gttaactccc
    56941 aatcaccaaa tgtaggcatt tgccgattcg aacttccgca taaaaccacc tcaattgaat
    57001 catctacgag tttcatcgcc ttagccgttt cctctgctaa ccgtccatat tcttccgctg
    57061 ttttatgtcc aatctgccaa ggaccgtcca tttcattccc taaacaccat gtcttaattc
    57121 caaaagggct ctcttgacca ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa
    57181 aattacaata ttcgactaaa ttacgagctg catctactcc acgagtaccc agattgacag
    57241 ccatattgat ttcggccccc acatgctgac accagttata aaattcatga atcccaactt
    57301 cattcgtttc aattgtccgc caagctaaat ccaaccttct aggtctattt gctatgggcc
    57361 caatactgtc ttcccaacga aatcctgaca caaaatttcc accaggatat ctaatcatgg
    57421 gaatgttcaa ttcttttacc aagtgaatga cgtcttggcg aaatccactt ttattagctg
    57481 atggatgatc tggctgataa atcccttcat atactgctcg tcccatatgt tcaataaatg
    57541 aactgtacaa ccgcggatca actttgccta cacgcttgac actactaatc aacattgtgg
    57601 cttccataac tgacctcctt taatttatac atgaataata cacctcgttg aaagcgttgt
    57661 caataatata aatgttaaac ttacttattt tttatcgatt ttaattaacc aaaatattaa
    57721 taatgattaa aacaaataat ttaatataac ttttattaac aaatatgtta atatataaga
    57781 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
    57841 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
    57901 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
    57961 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
    58021 tcgagccttc gtgtcgataa aatcgatatc agttttccag aaaaaatatt caatgctttt
    58081 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
    58141 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
    58201 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
    58261 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
    58321 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
    58381 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
    58441 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
    58501 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
    58561 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
    58621 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
    58681 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
    58741 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
    58801 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
    58861 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
    58921 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
    58981 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
    59041 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
    59101 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
    59161 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
    59221 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
    59281 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
    59341 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
    59401 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
    59461 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
    59521 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
    59581 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
    59641 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
    59701 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
    59761 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
    59821 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
    59881 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
    59941 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
    60001 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
    60061 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
    60121 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
    60181 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
    60241 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
    60301 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
    60361 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
    60421 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
    60481 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
    60541 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
    60601 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
    60661 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
    60721 ccttccactt ggattaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
    60781 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
    60841 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
    60901 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
    60961 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
    61021 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
    61081 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
    61141 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
    61201 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
    61261 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
    61321 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
    61381 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
    61441 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
    61501 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
    61561 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
    61621 aaaatggcga gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag
    61681 caggttacca taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt
    61741 tctatcacta caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc
    61801 tgtttcactc ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc
    61861 gcatagtcga tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt
    61921 ttatcatatg tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa
    61981 ttaatagccg tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt
    62041 aaattactat tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg
    62101 atttagatcc aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa
    62161 ttgaaccttt aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttcg
    62221 tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt
    62281 tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca
    62341 gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt
    62401 cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt
    62461 atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg
    62521 accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg
    62581 ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga
    62641 gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct
    62701 atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat
    62761 ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat
    62821 ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc
    62881 aagctcataa gaagtggttc tcttacagct ggcctgaata tcagccaaaa gcgcataaag
    62941 gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg
    63001 gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat
    63061 gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta
    63121 gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt
    63181 taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt
    63241 aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa
    63301 accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg
    63361 cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac
    63421 tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca
    63481 aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt
    63541 tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg
    63601 ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct
    63661 tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa
    63721 taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc
    63781 aaattcttca agataccttc gctctttttc tttatgatag cccgtcggta aaagtgtcac
    63841 tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg
    63901 atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct
    63961 ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt
    64021 ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc
    64081 tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga
    64141 ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct
    64201 tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt
    64261 tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt
    64321 aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt
    64381 atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa
    64441 acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat
    64501 taccaaagtc tcttatttta aattgaatac attataatga tactgtaaat atatctttat
    64561 tattagtgaa tg
//
LOCUS       DAZKCN010000004        61534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000004 DAZKCN010000000
VERSION     DAZKCN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.004"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8K3_000195"
     CDS             complement(59..187)
                     /locus_tag="ACG8K3_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9144110.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8K3_000196"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8K3_000196"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9144111.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8K3_000197"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8K3_000197"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9144112.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8K3_000198"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8K3_000198"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP9144113.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8K3_000199"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8K3_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP9144114.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8K3_000200"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8K3_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP9144115.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8K3_000201"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8K3_000201"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9144116.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8K3_000202"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8K3_000202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP9144117.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8K3_000203"
     CDS             10491..12050
                     /locus_tag="ACG8K3_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP9144118.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8K3_000204"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8K3_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP9144119.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8K3_000205"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8K3_000205"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9144120.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8K3_000206"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8K3_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9144121.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8K3_000207"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8K3_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144122.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8K3_000208"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8K3_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144123.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8K3_000209"
     CDS             17616..17771
                     /locus_tag="ACG8K3_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP9144124.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8K3_000210"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8K3_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9144125.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8K3_000211"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8K3_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP9144126.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8K3_000212"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8K3_000212"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9144127.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8K3_000213"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8K3_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144128.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8K3_000214"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8K3_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP9144129.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8K3_000215"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8K3_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9144130.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8K3_000216"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8K3_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144131.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8K3_000217"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8K3_000217"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9144132.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8K3_000218"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8K3_000218"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9144133.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8K3_000219"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8K3_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP9144134.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8K3_000220"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8K3_000220"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP9144135.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8K3_000221"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8K3_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9144136.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8K3_000222"
     CDS             34320..34841
                     /locus_tag="ACG8K3_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP9144137.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG8K3_000223"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG8K3_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG8K3_000224"
     CDS             complement(36284..36940)
                     /locus_tag="ACG8K3_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144138.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8K3_000225"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8K3_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP9144139.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8K3_000226"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8K3_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9144140.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8K3_000227"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8K3_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9144141.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG8K3_000228"
     CDS             complement(40491..41222)
                     /locus_tag="ACG8K3_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP9144142.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG8K3_000229"
     CDS             complement(41226..42491)
                     /locus_tag="ACG8K3_000229"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP9144143.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8K3_000230"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8K3_000230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9144144.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8K3_000231"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8K3_000231"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9144145.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG8K3_000232"
     CDS             47833..49335
                     /locus_tag="ACG8K3_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP9144146.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG8K3_000233"
     CDS             49337..50074
                     /locus_tag="ACG8K3_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9144147.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8K3_000234"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG8K3_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9144148.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8K3_000235"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG8K3_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9144149.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8K3_000236"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG8K3_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9144150.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8K3_000237"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG8K3_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP9144151.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8K3_000238"
     CDS             complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG8K3_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9144152.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ACG8K3_000239"
     CDS             complement(56756..56983)
                     /locus_tag="ACG8K3_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9144153.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8K3_000240"
     CDS             complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG8K3_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP9144154.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            complement(57969..58415)
                     /locus_tag="ACG8K3_000241"
     CDS             complement(57969..58415)
                     /locus_tag="ACG8K3_000241"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9144155.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            complement(58396..58992)
                     /locus_tag="ACG8K3_000242"
     CDS             complement(58396..58992)
                     /locus_tag="ACG8K3_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9144156.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            complement(59476..59637)
                     /locus_tag="ACG8K3_000243"
     CDS             complement(59476..59637)
                     /locus_tag="ACG8K3_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144157.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            complement(59630..60304)
                     /locus_tag="ACG8K3_000244"
                     /pseudo
     CDS             complement(59630..60304)
                     /locus_tag="ACG8K3_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            60827..61434
                     /locus_tag="ACG8K3_000245"
                     /pseudo
     CDS             60827..61434
                     /locus_tag="ACG8K3_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT        17077 a        13212 c        10646 g        20599 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttttaagtta
    59401 caataattat ctatttaata gtcttaattc attaaaataa aaaactcaac tttacaagga
    59461 tatggtttaa aaaatttatt tatccaaccc aactagtttt cgctgcttac gatttgcttc
    59521 atctgctacg taattcatta ctgttttaaa ttctttcgct gaggcgtttt ctgataaatc
    59581 agcgatactt ttattatact tcttcacgat ttgttgtact tgttctttct cagtcatgta
    59641 attcctcctc caactctttt gaagtatatt gtattggtag tactaattga atattatctg
    59701 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
    59761 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
    59821 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
    59881 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
    59941 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
    60001 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
    60061 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
    60121 tcacaacatg gttgtcataa ttttaagcta aacctagtgc aacctctgtt ttacctgctc
    60181 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
    60241 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
    60301 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
    60361 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
    60421 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg cttttttttc
    60481 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
    60541 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
    60601 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
    60661 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
    60721 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
    60781 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
    60841 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
    60901 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
    60961 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
    61021 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
    61081 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
    61141 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
    61201 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
    61261 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
    61321 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
    61381 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
    61441 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
    61501 ttatcaaaga agatacactc tatttatatt cata
//
LOCUS       DAZKCN010000005        59335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000005 DAZKCN010000000
VERSION     DAZKCN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.005"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG8K3_000246"
     CDS             complement(159..1469)
                     /locus_tag="ACG8K3_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP9144158.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACG8K3_000247"
     CDS             complement(1481..2899)
                     /locus_tag="ACG8K3_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9144159.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="ACG8K3_000248"
     CDS             complement(3139..4707)
                     /locus_tag="ACG8K3_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP9144160.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="ACG8K3_000249"
     CDS             complement(4711..5121)
                     /locus_tag="ACG8K3_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP9144161.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="ACG8K3_000250"
     CDS             complement(5118..6041)
                     /locus_tag="ACG8K3_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP9144162.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="ACG8K3_000251"
     CDS             complement(6038..6916)
                     /locus_tag="ACG8K3_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP9144163.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="ACG8K3_000252"
     CDS             complement(6903..8240)
                     /locus_tag="ACG8K3_000252"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP9144164.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="ACG8K3_000253"
     CDS             complement(8243..9346)
                     /locus_tag="ACG8K3_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9144165.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="ACG8K3_000254"
     CDS             complement(9420..10928)
                     /locus_tag="ACG8K3_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9144166.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="ACG8K3_000255"
     CDS             complement(10894..11586)
                     /locus_tag="ACG8K3_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9144167.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="ACG8K3_000256"
     CDS             complement(11608..12582)
                     /locus_tag="ACG8K3_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144168.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="ACG8K3_000257"
     CDS             12882..13754
                     /locus_tag="ACG8K3_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144169.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="ACG8K3_000258"
     CDS             complement(14212..16410)
                     /locus_tag="ACG8K3_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144170.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="ACG8K3_000259"
     CDS             16642..17634
                     /locus_tag="ACG8K3_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9144171.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="ACG8K3_000260"
     CDS             17694..19115
                     /locus_tag="ACG8K3_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9144172.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="ACG8K3_000261"
     CDS             19108..20607
                     /locus_tag="ACG8K3_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP9144173.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="ACG8K3_000262"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="ACG8K3_000262"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9144174.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="ACG8K3_000263"
     CDS             22199..22882
                     /locus_tag="ACG8K3_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP9144175.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="ACG8K3_000264"
     CDS             22884..23720
                     /locus_tag="ACG8K3_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9144176.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="ACG8K3_000265"
     CDS             23731..25485
                     /locus_tag="ACG8K3_000265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144177.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="ACG8K3_000266"
     CDS             complement(25761..26102)
                     /locus_tag="ACG8K3_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9144178.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="ACG8K3_000267"
     CDS             complement(26170..28341)
                     /locus_tag="ACG8K3_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9144179.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="ACG8K3_000268"
     CDS             28552..29409
                     /locus_tag="ACG8K3_000268"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9144180.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="ACG8K3_000269"
     CDS             complement(29480..30337)
                     /locus_tag="ACG8K3_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9144181.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="ACG8K3_000270"
     CDS             complement(30322..33024)
                     /locus_tag="ACG8K3_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9144182.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="ACG8K3_000271"
     CDS             complement(33037..34326)
                     /locus_tag="ACG8K3_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP9144183.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="ACG8K3_000272"
     CDS             34476..35522
                     /locus_tag="ACG8K3_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9144184.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="ACG8K3_000273"
     CDS             35524..37677
                     /locus_tag="ACG8K3_000273"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP9144185.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38150..39601)
                     /locus_tag="ACG8K3_000274"
     CDS             complement(38150..39601)
                     /locus_tag="ACG8K3_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9144186.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39598..41322)
                     /locus_tag="ACG8K3_000275"
     CDS             complement(39598..41322)
                     /locus_tag="ACG8K3_000275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9144187.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41315..41929)
                     /locus_tag="ACG8K3_000276"
     CDS             complement(41315..41929)
                     /locus_tag="ACG8K3_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9144188.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42274..43731)
                     /locus_tag="ACG8K3_000277"
     CDS             complement(42274..43731)
                     /locus_tag="ACG8K3_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9144189.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43772..44692)
                     /locus_tag="ACG8K3_000278"
     CDS             complement(43772..44692)
                     /locus_tag="ACG8K3_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144190.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44704..45651)
                     /locus_tag="ACG8K3_000279"
     CDS             complement(44704..45651)
                     /locus_tag="ACG8K3_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144191.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46130..46849)
                     /locus_tag="ACG8K3_000280"
     CDS             complement(46130..46849)
                     /locus_tag="ACG8K3_000280"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP9144192.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46898..48544)
                     /locus_tag="ACG8K3_000281"
     CDS             complement(46898..48544)
                     /locus_tag="ACG8K3_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP9144193.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48723..49313
                     /locus_tag="ACG8K3_000282"
     CDS             48723..49313
                     /locus_tag="ACG8K3_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9144194.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49405..50910
                     /locus_tag="ACG8K3_000283"
     CDS             49405..50910
                     /locus_tag="ACG8K3_000283"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP9144195.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(50994..52346)
                     /gene="celB"
                     /locus_tag="ACG8K3_000284"
     CDS             complement(50994..52346)
                     /gene="celB"
                     /locus_tag="ACG8K3_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9144196.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52346..52864)
                     /locus_tag="ACG8K3_000285"
     CDS             complement(52346..52864)
                     /locus_tag="ACG8K3_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144197.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52880..53206)
                     /locus_tag="ACG8K3_000286"
     CDS             complement(52880..53206)
                     /locus_tag="ACG8K3_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP9144198.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53219..55198)
                     /locus_tag="ACG8K3_000287"
     CDS             complement(53219..55198)
                     /locus_tag="ACG8K3_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9144199.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55219..55533)
                     /locus_tag="ACG8K3_000288"
     CDS             complement(55219..55533)
                     /locus_tag="ACG8K3_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP9144200.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55701..55994
                     /locus_tag="ACG8K3_000289"
     CDS             55701..55994
                     /locus_tag="ACG8K3_000289"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP9144201.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56072..57184)
                     /locus_tag="ACG8K3_000290"
     CDS             complement(56072..57184)
                     /locus_tag="ACG8K3_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9144202.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57337..58185
                     /locus_tag="ACG8K3_000291"
                     /pseudo
     CDS             57337..58185
                     /locus_tag="ACG8K3_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(58538..>59335)
                     /locus_tag="ACG8K3_000292"
     CDS             complement(58538..>59335)
                     /locus_tag="ACG8K3_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9144203.1"
                     /translation="LDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFE
                     IERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGA
                     DGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSD
                     IRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17753 a        12133 c        10381 g        19068 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg agggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt
    38041 ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag
    38101 atttctagac aattttttat aagttatttc attacattgg ctttttcttt tatgattctc
    38161 cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga
    38221 aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt
    38281 gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt
    38341 ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg
    38401 acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt
    38461 ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct
    38521 gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct
    38581 catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc
    38641 gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt
    38701 tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg
    38761 tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa
    38821 attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag
    38881 aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa
    38941 aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc
    39001 cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag
    39061 tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc
    39121 ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca
    39181 gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga
    39241 ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg
    39301 cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa
    39361 attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg
    39421 gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt
    39481 ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct
    39541 tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca
    39601 tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat
    39661 ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga
    39721 accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc
    39781 agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac
    39841 tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc
    39901 aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat
    39961 aaaatgatag gctactcgat taggataacg catttgatac aaatagacat atttttcgca
    40021 aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa
    40081 agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat
    40141 tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg
    40201 catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat
    40261 gctatccaac atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt
    40321 tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat
    40381 agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc
    40441 taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact
    40501 ttttttatct agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa
    40561 attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat
    40621 gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag
    40681 tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc
    40741 taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc
    40801 tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt
    40861 tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac
    40921 gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa
    40981 agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat
    41041 gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt
    41101 tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc
    41161 atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat
    41221 aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt
    41281 tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat
    41341 ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga
    41401 caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa
    41461 taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca
    41521 gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa
    41581 ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga
    41641 tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa
    41701 atagcagatt gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga
    41761 tttctttata ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac
    41821 ctaatccaaa gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta
    41881 taatgaccca gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc
    41941 caaacattaa gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga
    42001 cctgattatt agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg
    42061 aataccgagg gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag
    42121 tttcgagtag taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc
    42181 ttaataccga gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt
    42241 cttttgtcac aacccctttg tctttattca ttattattct tgtcggaact catcatattg
    42301 tttttgcatt tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt
    42361 cggtaatgtt tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat
    42421 gacattagaa atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa
    42481 aatcggtgaa gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt
    42541 aatagactct tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt
    42601 atttggctga taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca
    42661 tgcttctccc tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag
    42721 ccctagaagt tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc
    42781 catttgtgct tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg
    42841 ttttgctgca tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg
    42901 cattagccaa gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg
    42961 aatgagtcct tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt
    43021 atcgtattgg ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt
    43081 attccctaaa ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc
    43141 tgccgtgttt ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga
    43201 ttggattcca tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac
    43261 ttgttgagca aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt
    43321 tcctttaata tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa
    43381 ttcagtcaaa tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc
    43441 gatatcataa ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca
    43501 gccaatgtaa ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac
    43561 ttccatcaat tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc
    43621 cgaattgcta gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc
    43681 caacaacaac gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc
    43741 tcctaatttt atgatttata tttttgtttc tttattcttt tactccgccg attgttagac
    43801 ctttgacaaa atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga
    43861 ctacgatcgc cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc
    43921 ttcctgctcc tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat
    43981 actgtagtgg aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc
    44041 agtaacccaa tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa
    44101 cgatctgaac aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg
    44161 aatcagagac cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag
    44221 ccattggcaa gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa
    44281 gataattggc caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa
    44341 aacgacggaa tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc
    44401 tattgatcag tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt
    44461 tatcctgcat ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa
    44521 aactgtaccc gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata
    44581 taaatggcaa aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag
    44641 ccgtcttatt gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc
    44701 tccttagaat aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa
    44761 caagacaaag ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc
    44821 tgtggatgtc aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc
    44881 accggaattc ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac
    44941 ggctaaaatc gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat
    45001 ctgctgccat ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc
    45061 cataacagat gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa
    45121 caccaagata aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg
    45181 tccagtgatc cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta
    45241 actgataatg acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac
    45301 gagtcgttta tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt
    45361 caaaaggata aatgctacat tatataaaat tgtattttta gtgatgagaa atgcttgatc
    45421 cgaagaaaat aaaaatttga aattttccca acccacccaa ggactttccc tcaagctagc
    45481 caagaaccca tctgctgaaa tatgaaaatt tttgaatgct acgacattcg ccattacagg
    45541 tatgtaaaag aaaaaaatca tccaaagcat acccggtaca gccatcagga caagtgcttt
    45601 ataacgccat agattcataa aaaaatgatt gaaccccttt cgttttttca tctaaaaacc
    45661 cctttcgatt cattcgcaac gtaattatag cgctttcaat aatgagaata aataaacaaa
    45721 gtatagggaa aatgaaacaa agtataggag aggttgtgaa atcagcgttt tggcctgagt
    45781 attagagaaa aaaacgggaa aatagttcct catattttgc cgttttttga gctaataccg
    45841 agggtcagct gattgaacac cgtttatcag aggttgtgaa ttcagcgttt tggcctgagt
    45901 attagggaga tcgtgtcctt actatctaac tctgagtcac aaggaacaat ggggactgta
    45961 acaaaagtct tgtcacagtc ccttattccg aacaaacggt gttccagaag tagcttctac
    46021 ggaaataagc cgaaatttca caaaaaagcc atccgaaagt cagacggaca agtctgcttt
    46081 tcagatggcg ctacaactac tctgcttatt cttttagcta tccattcaat tatttttctc
    46141 tcacttgttc tagctcttct ttggtcatgg gtcgatattg ccctttattc aaatctttag
    46201 gcagccaaaa ttttcccata cggattcttt ttaaatataa gacttctttt cctactgctt
    46261 tgaccattcg cttaacttgg tgaaactttc cttcatgcaa aatcagacgg ataattgaag
    46321 tttctgtctc ttgatctact tgatcaattt ggagttttgc cggcaatgtc tgctcatcgc
    46381 catcaataat cagtccttct gcaaataaac ggatatcttc ttctgtcatt ataccgctaa
    46441 cctcagctag atattctttc tccacatgtt ttttaggtga tagtaactga tgggcaaaca
    46501 ccccgtcatt cgttaaaatc aagaggccct ctgtgtcttt atctaatcgg ccgactggaa
    46561 aaagatcttc ccgataatcc tggtcttcca ataaatcgat tacggtttga tcatgattat
    46621 cctgagtagc agaaatgact cctgcaggtt tatgcaaaat gtagtaaaaa tctttttgat
    46681 acgtaatgat ttctcctaaa taagtaaccg catcttcgaa ttcatttact tgcgctttat
    46741 cgcttttcac cacttgtcca ttgacttcga tcgttccttt tttgattaat cctttgactt
    46801 ctttgcggct gccaagtccc atatctgcta aaaacttatc taaacgcatg cgtttccccc
    46861 atttcaaaag agcatcaatc tagctcttct gattcactta ttttattttt aatttatgtc
    46921 gtaaacgcgc tattcctggt cctagtagtc tttctgccaa gcggattttc aaagctaaat
    46981 aaccatacac taaagcccct atacctgcta ctacaagaat caacaataga gactggaatt
    47041 tgctatcttc gtttaaaaaa gcaccaaaaa tcatctttgt gatccacgaa gccaaaagca
    47101 taatcaaggt aagaataaag atcagcaatg tcctgcgcca gacaaaactt ggtttaaagc
    47161 gggctacttc atggattctc tttaagatca aggtacaaga cacagcaaat ccgatcattg
    47221 tcgccaataa agggccgtat acttcaaata aacgaatggc tgggtattga agaaggagtt
    47281 tcactagaaa cccaataacc aaataattaa ttgccgcctt gttttcaaac attccttgca
    47341 gcatgttgga aactagcata tatagtccta ggaagagtcc tacaaaactt gcttggatca
    47401 gtacattact tcctagagaa tccggtgtat aaaataatgt atttagcgga taggcaagta
    47461 acataacacc aaatgtcgcc ggaaacataa aaaatgaaaa caactgtaaa tttccgcttg
    47521 tcaattttgc cagacctact cgatctttca acgtaacagc ttctgtaatc aacggtaacc
    47581 cagtagacgc gatcgatgtt gccaatgcaa taactaccat cgtcaactta tctggattgg
    47641 cactaaaaat cgaaaaaaga tccagtaact gtgcattgga atactcggtg gatgcagaca
    47701 tgatcttcat aaaagtgaac tgatcgacta atttgaagat cgttacccct gaacccacaa
    47761 taataaacgg aatcgcttct ttaacggtat ctaacaacaa ttccttcgtt gcaatattta
    47821 ttttgttttc gctgtagtga ataaacgccg atgtatacgc ttgatgtttc ttcaaaaaat
    47881 acaaaaggac aagaatactt gctagcatac cgataaatgc cgcgaaagtt gattgcgtga
    47941 ccgccgtcac atagtcacca tccatgactt tcatgatgat gaatgtagcc aatagcatat
    48001 aaaaaatacg tgccacctgt tctacaatct gcgacagcgc ataaggcatc atttcttgat
    48061 tgccttgcaa ataccctcgt atcacactca tacaaggaaa aaccaataca gcgatactca
    48121 aagaacgcat cgttggaatc agttcttctc cgccaccaga agcatgagct agccaaggag
    48181 aggcgatgta catgaacaaa gcaaacaaca ggcctaaccc gcccatcatc tgtaatgcac
    48241 ggataaataa tcgtcgtgaa gtgccatatt cattcaatga attatagcgt gcagtttgtt
    48301 ttgcaatcgc agcaggtatt cctgctgtag agatcagtag aaacaatgca tatatattgt
    48361 atcccatatt gaataatcca ttggcagcct tagcattttc gcccatccaa gcataccagg
    48421 gaatgatata gacagcgcct agcaatcttg aaatgatatt actggcggtc atccaagcag
    48481 agcctcgtgc cattttttct tgcggtgtta attgatctaa tgctggtttt ctttgattat
    48541 ccatcggtca gattccaact ttctacaaaa cattactatc attttaattg gtaaaacaga
    48601 aactggcaat cgattttata taacttttat aagaacaatt gtttttttga attcggtttc
    48661 gtttttttgg taaagtaaag aaatatacag ccttttatgg ctaacattta taggagatat
    48721 caatgacaca agaaataaaa aaactacccg ttatattttt aggtcttttc tttatcacag
    48781 ttggattgaa ttggttcttg ctccctcacg atatagcttc cgccggagtg ggcgccatcg
    48841 gacatttagt tgaaacggcc ttttctatcc aacgctcagt tactgtttgg agcatcaatc
    48901 ttatgatgct gcttctagcc gctgtcctat taggaaaatc agtatttttt aagactgtag
    48961 cgggaagtct gctttttcct gttgttttag agattgttcc aaaagtggaa atgatctcct
    49021 cacatttctt ttcactgatt tcaggaagtc ttttgttcag tcttggggtt tacacattgt
    49081 acacagtcgg ggcctcaaat ggcggtataa cgataccgcc gattattttc cacaaatttt
    49141 ttcggcttcc aatgccgcaa ggacttttac ttaccaatag tcttatcgtc tttttgaatt
    49201 attatgtttt tggattgttg gagacactat tcgttctcct atctattaca gtcagttcat
    49261 tgtttatgaa aatactcatc cgcttgtccc ctgtgtcaag aaaatttgaa taaagctgga
    49321 ttttgtcata gcggaaacaa acgatatatt aaaaagagtg tgctataata aaccaagaaa
    49381 ttgaataaag cgagggatca atcaatgagc ttctctttag aagacatccg tcatttacta
    49441 ttaaaagaaa atctattaaa agaatttgtt tcaacaagag gctggcatct tgatgtacca
    49501 gaaaaagctg aatttcttca gttaagttat gattcaagaa aagctgatgc ttctactttg
    49561 tttttctgta aaggaatgaa tttcaaagaa gaatatttgg attcagcaat cgaacagggt
    49621 attcaatatt atgtatcaga acaaccatac gaaaattcag cagtaggaat catcgtaacg
    49681 gatatccgta aagcaatggc gcttcttgct atggcttttt acgactatcc gcaaaataaa
    49741 ttgaaagtga tcggattcac tggaacaaaa ggtaaaacaa ctgctgcgta ctttacaaaa
    49801 gcaatcttgg atcacacaac aaataaaaaa acagcattgc tttctactat gaatacaacg
    49861 ttagatggca aaacatattt caaatcccat ttgacaacgc ctgagtcatt agatttatat
    49921 cgtatgatgt cagaagcagt tgaaaatggt atgactcatc tagtgatgga agtttcttca
    49981 caagcttaca aaacacaacg cgtatacggc ttgactttag atgtcggaat cttcttgaat
    50041 atctcgcctg accatatcag tccgatcgaa catccgacat ttgatgacta tttctattgc
    50101 aagcgacaat tgatcttgaa ttcaaaaaca gttgttttaa atcatgaaag tgactatttt
    50161 gatttactta gggaaacagc cgatctattc cagattccaa cgatcactta tggtcgttcg
    50221 gaagaaagca attatcaagt gatccgttca gataaaggta ctcacggttt tactttatct
    50281 tctcatgaag atcgcttagc tatctgcgat actgcttatg atattttgct ggctggagga
    50341 ttcaaccaag aaaatgcagc cagtgcgatc attgccgcag cactaacagg tgcctccagt
    50401 caagatgcac aagacggatt aaaagaagct cgtgtaccag gccgaatgga tcaattgatc
    50461 caatccaatg gagcgcatgt ctatgtggac tatgcacata attatctaag cttgaaaaca
    50521 ttgctgaaat ttgccaaaaa tgaacatcca gatggccgtg taattgttgt attaggcagc
    50581 ccaggaaaca aagcaatctc ccgtcgacat gattttggaa aagtgctatc agaaacagct
    50641 gacatcgcct ttttgacggc agatgaccca gcatttgaag atccgaaaaa aatcgcggaa
    50701 gaaatcaatg aagcaattac taatccagat ttgatcgttc attatgaaat ggatcgtcct
    50761 gaagcgattc gacgcgcttt agcagaaagc gctccgcaag attcagtcgt tattgcggga
    50821 aaaggcgtgg atccctatca aaaaatcaat ggtgtagatg aaccttatga aggggattat
    50881 gcaatcgtga agcggttgat tgaagaataa tggtatttat atactagtgg tttcccattt
    50941 ctaagaaaaa aagggactgt gattttttgt cacagttcct tttttcttag aaatcatttt
    51001 aacttttcac tagctaattc tgctgccttt tctctttctt catttaataa tttattatca
    51061 tagaatttga taaatgggaa ccagataatg aatgcgatga ttgcacaaat gactgctaac
    51121 acagcagctt tccaatcacc accactacca acaaatgctc cgatcccgac tggtgaaggc
    51181 cacggcatct gtgcaagcat cggtttgacg atttccagcc gaatggcaaa ataagcgagt
    51241 gtagcagaag ccatcggagc taagaaaaac ggaaccgcta aatagggatt gtagacgatt
    51301 ggcatcccga aaatgattgg ttcattgata ttgaataatc ctggtacaag tgaggctttt
    51361 cctaacactt tcagctgatc agatttcgcg aggaatgcaa taaagatgat caatcctaaa
    51421 gtcgcacctg atccaccaac ggttacataa gaattgttga attctcctgc caacggataa
    51481 tttgcgcctt gtgcgttagc tgccatatta gctaatacga tcggggtcaa gaaagaagtg
    51541 atgatattcg ctccatggat ccctacgatc cacaatgcat ggatcaagaa ataaatcacc
    51601 atgacaccaa gccatgtatt cgtcaactgc gtaacaaacc caaatgggat agcaatcatt
    51661 ttgaagatat ccgttcctag tgcaactaat aatccattga tgatcatcac cacaaacgca
    51721 atgacaaatg ttgggatcag agcagtgaaa gaacgagaaa ctcctggcgg aacaacatct
    51781 ggcattttta tgacccaatt tctcttgaca cacatacaat acaattcgac cgcaataatc
    51841 gacataataa tagctgtaaa gattcctgaa gttcctaaac gttcgacaaa agagcccatc
    51901 ctaataccat ttactacagt ttctgtctca ttgatagaag caatcaagct gattttacca
    51961 tcagtcatga tcaattcagg gatacacata aagaatgcaa aaacagatag taatgccccc
    52021 gtgagcggat taacatttaa tttttgttca tctcgttcaa ttgacgttaa ttcataagca
    52081 aacacaatgg caaagtaaat agataaaatc cccatcgtca ttgtattgac gatcatgtac
    52141 aagtcactaa ctttgaaaaa agttgcatta aaaaatcctt ctaaaaatgg gaacggtaat
    52201 ggcaaaatat ttaaaaccaa aaacatcgag cctacaatcg tgaaaggaat agaagccatc
    52261 cccgctgcca tgacagcccg aacgatttta agttgcgcaa ttttccccat aggtcccatc
    52321 aggtatttct gcaaaaaatc aaacattagt tattctcctt tcctaaatat aacgcttttt
    52381 catattgttt aatacggata tactgttggt cttggttgag tgaaattttc tttaaacgat
    52441 actgtataaa tgcactggca aaaataccgc ttacaacaag aatgaaaaca aataactgtc
    52501 ctccattgtt atttgccata aaaggataca agaatgtaaa tcctgaggta aacaacactg
    52561 gaatcactaa taaattaaag acactctgga acaaaaaata ccactttgtc catggaacgt
    52621 tcgtttggtg ttttcgataa agctttactt gttctcctac tgcaggcaaa atagctaaaa
    52681 tcagaatcat cggtaaacta cttcctaaac ttttagctga tagaagcaga atcatccatt
    52741 gcatgttcat gaaaaagaaa aaggcagtca ggtaacgtac gattagataa cgattgaaat
    52801 acatgttttt caaactaagt tgcttttttt ctaattcgat tttccttttt actgtatcct
    52861 gcacaatgat ccttcctttc tatttatttt tttgatacaa ataattcatt tcctgtgcaa
    52921 cttccaataa tgtcatcgtc gtcatcagat gatcttgtgc atggaccatg ataatttcca
    52981 tttctatttt tgtcccgctg gcatattctt tcagaagttg agtttgtgac tgatgcgcat
    53041 gaagaatctc ttcatttgct tgtcgcaact tctcttctac ttcttcaaac atctctttac
    53101 gcatcgagcg aaatgcttca tgtatcagtg tccgtgcatt tccgctatgg aaaatgatat
    53161 caaaagaagt cacttgtagt gtttcactgt ttattttttc agccatctgt tttcctcctt
    53221 attcgatcag tttgataaat ttactgctga aattctcaaa aataggttca gctaagattt
    53281 cctgctgtac ttttagctgg tcaactaacc taacgattgc tttagtcacg accgtaattc
    53341 cttcattttc gacataagaa ggcgaaacta aaaaaacaaa atgaatttct tgatattgat
    53401 cccactgcat cccagaagga atcaatgcaa cggctacctt tgtttcgatt ccaactggaa
    53461 cagcgggatg tgggactgcc actgtatcac tgaatatgat ttgtcccata ttttcacgtt
    53521 gctggatctg ctttcgcatt tctcttatat atccttcttc ttcatttatg ctcagctttt
    53581 gcagcaaatc atgaagaatt tcttcctttt tcggttcgta cgtataagtc ttgaaaaatt
    53641 tttcagacaa ctgttcggtg taaatctgct ttttttgctg tagagaaaat gctggtatcg
    53701 gtctatctga ggaatgatag ggtatagatt ctccaactac ctttcgtatt ttacgaacat
    53761 catcttcatt caaaaaaata ctgacatgta aaactggaat tttgaaaaac attgtcgaaa
    53821 gatcaataga agaaataatc aggtcgattc cttttaaagt tgtttcattg atctcataat
    53881 agccttttac acttaccact gtaatatttt taccgaattc actgactacg cgattcttta
    53941 atagttgggc actgccgtaa cccgttgcac agataatcaa tgcatgtagt ttatgcgcag
    54001 ctctttcttt ttccaatgcc gccatgagat gcaaagccaa ataggcccat tcatcttcat
    54061 tgatggtata gtttttcaaa gccggcatgt tgctgaaatg cctttttact gcgtcaaaga
    54121 ctttgggatt ttctttcttt atctcctcag tcaatgggtt ctcaatgcga atacctcggc
    54181 tcaatcgaat catcatcggt gacaaatgat cgagtagtcc attgaaaagc tggagatcct
    54241 ctactaatga aaaatgaaat tcctgagaca atcttttcag aactttcgct aattcctttg
    54301 atagttcctg atcattttga ttttctttgt gattcgactt agccatgaga tgtaatgtca
    54361 aataagcaat ttcttcttcg ggaaacgata cacctgcgat tacctcaata cgttgaacga
    54421 tccgctgcgc cacttgatac tctactcgct tagaaacttc ttgttcgatg cctaattcct
    54481 tgatttcaaa cccttctcgg agccgtttga tccccaaagc caagtgtaaa gtcagattct
    54541 gtatgataaa atccgaaact tttaacttag cttctcttgt ttcatcgaga atgataatga
    54601 ctagctcttc aaaactgatg tctgggaaaa aacggctttg ccctaaatat tctttcaatg
    54661 aatttccata atggtttcca aaaaaagtat ccatgataaa atgccgtcga tttctttctt
    54721 ccccttcgat ccatgtccct tgaccatgct tagaaacgat ttttagtgaa tatggaatca
    54781 atcgctcctt gatttcttgc atgtcctttg tcaaactgga acgactgata taaagctctt
    54841 ctgccaaatc atccataaaa agaatggcat cttccaaaag caattggttc aatatatact
    54901 tttgtcgatc ttcggactca taaaatcctt gcggttctct agcatatcca ggtactattt
    54961 cttgccgact caaaaacaag tcaaatgtca gtctttgtaa aatatgcagt tggtagccat
    55021 aacctggttt agcaatgatc ttacctccgt ttttttctac taactctttg atccgctgca
    55081 agtaattacg gattgtccga tcagataata acaattcaga agctaaacgt tgactggtga
    55141 catactcctt cttgtgatca atcaaatatc tcaacagcaa tttttccttc tgtttcatta
    55201 tcatccactc ctattccatc atatgttctc ttcgatcacc tctgctaact tctggatgcc
    55261 tgtagggatt gggacatacg cttggaaagg gatactaaca acaggctttc caacagattc
    55321 tcctaatttt ttgaatttat ccaaaaacat cgtcgtttgc gggctgatca gaaataaatc
    55381 aaaatcacta ttcttaatca tattatcgcc ttctgtagct gaaactgcat ctacgagtac
    55441 ctcttctctt ttttgcttaa agaaatccgt tgtctttgag gccatcatcg aggatgacat
    55501 tcctgccgca caaataatca atgctttttt cataaatcaa tcactccttt tatttttata
    55561 aaccaagcat atcttgtaag cgttttctat tcaaaatata tatttccgta gcattgcgga
    55621 aaaaatgtgt atcatatctt gaataataat ttctggcacc gtgatatact agtgatagaa
    55681 ttttttcagg aggggattta gtgagtatca cagtgaatat tttgtataca agtacaaatg
    55741 gacaagccag aaaatttgca gaagaaatgg tcgaaagaaa cattgtcaaa gcgattcgtg
    55801 cagaagaagg aaacgagaaa tatgactatt actttccact agaagatcca gaatctcttt
    55861 tactgatcga ccgttggaaa gatgaagaag caatcgaaaa gcatcacaaa tccgaaatga
    55921 tggctcaaat aaacgaactc cgtaaaaagt ataaattaaa aatgaaagtc gaacgtttca
    55981 ctgatttacc gtaaaaagat acacatggaa tgatagcaat ttatcatcca tgtgtatctt
    56041 cactttttcc ttatttttta ttcgatctca tctaatgatt caaaccgtgt ttttctctta
    56101 tttttcggat aagtatttca gtcaccccaa tatacatgat tcctaaacat accccaagga
    56161 tattctcaaa aatgcggatc gttactgctc ctggaacccc gaataaactg gcagtgattg
    56221 ccaaagcacc aaaagaatta aaaactgttt tatatctata ggtagaacaa acacctaaag
    56281 ctagaccacc taatataccg acccagttaa aaggaataaa ttggctgata ccaataaata
    56341 aaacacatcc aatcaatgag ccaatgattc ggtctactgc tcgttctttg aaaccaatcg
    56401 acatcaatcc atagctagat acaatagaag aaaatgcaaa ccctgcccac atcaatcgct
    56461 gaaaagggac atactctcct gcaacgatca aaagactaat gccaaatgca tagatgagca
    56521 tccaaatttt ttcctgcgaa tacatgcctt tcaaaaaaat ctctttaaac aagcttttgt
    56581 ctttgttctt ttctcgatgt tttctgtaaa ggagaactga aaaccaacaa aaaaacaaaa
    56641 ataataagct acttgtttgt gtaaagaaat tcttgttcaa cgaatcttta ggtaacgaat
    56701 aaacaataaa gagatatgaa aagccgtaca aagaagcatt ccccattttg ggatcactag
    56761 cggttgccat taatatgctg cttagtaaaa taaaatgaat caacatgccc ataactgacc
    56821 atttgatcag ctggacatac ggtgctacta atagtgagaa catgacgatt gctaaaccta
    56881 tgatcgaatg ggaaatctta taaccaaagt caacaaaacg aatactcatc aacatacaga
    56941 ataaaaccac agcgtatggt ttatgcttcg attcaaataa tgttgtaatt ataatgataa
    57001 aacaaatggc aaacaatacg atgaataatg atcgtaaaaa caatgaacaa atatacatgc
    57061 acttttcttt tttagaagct gcttctcgta ttttttgttt taaaataaac ggatctagtt
    57121 gtagagactg ataaaaatcc acctgttgtc gtcctttctt tttactttta ttttttacag
    57181 tcatctatga caggttgtaa atactatgga acatcatatg gcgttgtcat tttcttgtca
    57241 actatttttc ctattcatgt ttttataaaa aaacatatcg ttttcttgta tgattaaatc
    57301 accacaaaat aaacaactga gagaacgata tgctttatga atggttctat caaaaaaatt
    57361 ctgagatcaa tagacaaaaa tttgatgatt acagaggtct cttacgaaac cttacagaag
    57421 aaaaaaacat tgatcgttgc tgcagttctc ttccctgctc catgtgtctg tagaaattgt
    57481 ggttctgctg tggtagatgg aaataggaaa ttaatggtgg tgaaaaacaa gaagaaggaa
    57541 accattgtcc actttgaata atataatcat atgccaatgg ttatacgcct aaaaaagcag
    57601 cgctatacct gtaaaaactg tcgaagccat tggaacgctc aaagttattt tatccgtcca
    57661 aggcactcga tttcaaatca tgtcagacat aaaatcactt ctttactgac tgaaaaagtg
    57721 tcgttatttt ttatttcgaa aagttgttag gtatctttga caaccgttat tcgcacattg
    57781 aaagagttga gaagctattt accaaagtct tccaaaagaa ttctgtcttg agtgttgagg
    57841 gttgtgaatt tcgttctcac gtttccgttg aggataaact gagttttatt tccgcggatg
    57901 gcgagacaga aaaattaatc gagaaaattt acctacacaa aaaattcctc gattaacaaa
    57961 ctatttcctt agctgcacta atcctaaaga aatagaattc ttagttacag acatgaacgc
    58021 cacctacttt cagctcacca aacatgttct accaaatgcg aaattggtga ttgatcgatt
    58081 acttggtttt tccacgcctt tcaaggaggc gtaaccttta ttcacgagtt ggttgaagct
    58141 ttccgagaca aaagacccga cttgtttttc tccttattga caaagagcca ttaaaactag
    58201 agaaaaaatt acaactgatc gctgattatt tgtagaaaga aaatcaaagt attacccaat
    58261 aaacttgagt cattaggcaa aaaacactgc gaaactcaag aatgattttc gcagtgtttt
    58321 tgtatatttt ttattaacta gtattactac aaaggtttat ttttcacttc tcaacgcagc
    58381 acaaaattgt ctttacttat ttactggttg ggttcaacct tctctactcc gatactaata
    58441 tttgtttgta taccccttct aaaatcggta gtttccattc aattgatggg tactagttga
    58501 ttttaaaaac tatgcttatt caatttttta ttttagctca tatatttcgt ttctttctaa
    58561 attttatact gatgaaaaca agtagcacac ccaatattga caatataaac tggctaatga
    58621 tggtttctcc agtttttgga agatgcttat ttccactgct tgttggattg tttcctctta
    58681 tatctgagct atcagcaact tttctattct cttttgttaa aaagatcgcc acttcttgtt
    58741 ctttttcgat cttgaaagtt actggtgtcg catccaattc ataccccgtt ggcgcttgag
    58801 tttctaccag ctgatatgct cctggtgcta agccatctat cgccagtttt ccatctgctc
    58861 ctgtggttag acctgactgc agaacggttc cattggcatc ttgtaactca aaaacagctc
    58921 cttgcaggac ttctccgctt tggtcatcga tctttgtcaa cacgacaccg cctggtgtaa
    58981 gtcgattttc tttcgtcaat tctaccaccg ctgtctgact tctttcgatc tcaaactcga
    59041 ttggtgtcgc atccaattca taccccgttg gcgcttgggt ttctaccagc tgatacactc
    59101 ctggtgctaa gccgtcaatc gccagttttc catctgctcc tgtggttaga cctgactgca
    59161 gaacggttcc attggcatct tgtaactcaa aaacagctcc ttgcaggact tccccgcttt
    59221 ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag tcggtttgct ttcgtcaatt
    59281 ctaccaccgc tgtctgactt ctttcgatct caaactcgat cggtgtcgca tctaa
//
LOCUS       DAZKCN010000006        58246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000006 DAZKCN010000000
VERSION     DAZKCN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.006"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..894
                     /locus_tag="ACG8K3_000293"
     CDS             13..894
                     /locus_tag="ACG8K3_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9144204.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            907..2517
                     /locus_tag="ACG8K3_000294"
     CDS             907..2517
                     /locus_tag="ACG8K3_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144205.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2594..3253)
                     /locus_tag="ACG8K3_000295"
     CDS             complement(2594..3253)
                     /locus_tag="ACG8K3_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9144206.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3536..4201)
                     /locus_tag="ACG8K3_000296"
     CDS             complement(3536..4201)
                     /locus_tag="ACG8K3_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP9144207.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4348..4953)
                     /gene="dhaL"
                     /locus_tag="ACG8K3_000297"
     CDS             complement(4348..4953)
                     /gene="dhaL"
                     /locus_tag="ACG8K3_000297"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9144208.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4981..5991)
                     /gene="dhaK"
                     /locus_tag="ACG8K3_000298"
     CDS             complement(4981..5991)
                     /gene="dhaK"
                     /locus_tag="ACG8K3_000298"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9144209.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5966..6361)
                     /gene="dhaM"
                     /locus_tag="ACG8K3_000299"
     CDS             complement(5966..6361)
                     /gene="dhaM"
                     /locus_tag="ACG8K3_000299"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9144210.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6515..6835
                     /locus_tag="ACG8K3_000300"
     CDS             6515..6835
                     /locus_tag="ACG8K3_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9144211.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6947..7804)
                     /locus_tag="ACG8K3_000301"
     CDS             complement(6947..7804)
                     /locus_tag="ACG8K3_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP9144212.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7912..9216)
                     /locus_tag="ACG8K3_000302"
     CDS             complement(7912..9216)
                     /locus_tag="ACG8K3_000302"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9144213.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9213..9722)
                     /locus_tag="ACG8K3_000303"
     CDS             complement(9213..9722)
                     /locus_tag="ACG8K3_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144214.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9897..12032)
                     /locus_tag="ACG8K3_000304"
     CDS             complement(9897..12032)
                     /locus_tag="ACG8K3_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9144215.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12115..13770)
                     /locus_tag="ACG8K3_000305"
     CDS             complement(12115..13770)
                     /locus_tag="ACG8K3_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9144216.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13772..14086)
                     /locus_tag="ACG8K3_000306"
     CDS             complement(13772..14086)
                     /locus_tag="ACG8K3_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9144217.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14256..14516
                     /locus_tag="ACG8K3_000307"
     CDS             14256..14516
                     /locus_tag="ACG8K3_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP9144218.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14530..14844
                     /locus_tag="ACG8K3_000308"
     CDS             14530..14844
                     /locus_tag="ACG8K3_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP9144219.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15574..16128)
                     /locus_tag="ACG8K3_000309"
     CDS             complement(15574..16128)
                     /locus_tag="ACG8K3_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9144220.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16254..18183)
                     /locus_tag="ACG8K3_000310"
                     /pseudo
     CDS             complement(16254..18183)
                     /locus_tag="ACG8K3_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18489..19205)
                     /locus_tag="ACG8K3_000311"
     CDS             complement(18489..19205)
                     /locus_tag="ACG8K3_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9144221.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19206..21029)
                     /gene="glpO"
                     /locus_tag="ACG8K3_000312"
     CDS             complement(19206..21029)
                     /gene="glpO"
                     /locus_tag="ACG8K3_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP9144222.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21043..22539)
                     /gene="glpK"
                     /locus_tag="ACG8K3_000313"
     CDS             complement(21043..22539)
                     /gene="glpK"
                     /locus_tag="ACG8K3_000313"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9144223.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22675..24135)
                     /locus_tag="ACG8K3_000314"
     CDS             complement(22675..24135)
                     /locus_tag="ACG8K3_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144224.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24125..25420)
                     /locus_tag="ACG8K3_000315"
     CDS             complement(24125..25420)
                     /locus_tag="ACG8K3_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9144225.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25752..26429)
                     /locus_tag="ACG8K3_000316"
     CDS             complement(25752..26429)
                     /locus_tag="ACG8K3_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP9144226.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26459..27550)
                     /locus_tag="ACG8K3_000317"
     CDS             complement(26459..27550)
                     /locus_tag="ACG8K3_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9144227.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27651..28085)
                     /locus_tag="ACG8K3_000318"
     CDS             complement(27651..28085)
                     /locus_tag="ACG8K3_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9144228.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28669..30126)
                     /locus_tag="ACG8K3_000319"
     CDS             complement(28669..30126)
                     /locus_tag="ACG8K3_000319"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9144229.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30123..32018)
                     /locus_tag="ACG8K3_000320"
     CDS             complement(30123..32018)
                     /locus_tag="ACG8K3_000320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9144230.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32076..33851)
                     /locus_tag="ACG8K3_000321"
     CDS             complement(32076..33851)
                     /locus_tag="ACG8K3_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9144231.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33901..35397)
                     /locus_tag="ACG8K3_000322"
     CDS             complement(33901..35397)
                     /locus_tag="ACG8K3_000322"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9144232.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35591..37213)
                     /locus_tag="ACG8K3_000323"
     CDS             complement(35591..37213)
                     /locus_tag="ACG8K3_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9144233.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37541..38854)
                     /locus_tag="ACG8K3_000324"
     CDS             complement(37541..38854)
                     /locus_tag="ACG8K3_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9144234.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39099..39323)
                     /locus_tag="ACG8K3_000325"
     CDS             complement(39099..39323)
                     /locus_tag="ACG8K3_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144235.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39343..39765)
                     /locus_tag="ACG8K3_000326"
     CDS             complement(39343..39765)
                     /locus_tag="ACG8K3_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9144236.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39908..40618)
                     /locus_tag="ACG8K3_000327"
     CDS             complement(39908..40618)
                     /locus_tag="ACG8K3_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP9144237.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40636..41133)
                     /locus_tag="ACG8K3_000328"
     CDS             complement(40636..41133)
                     /locus_tag="ACG8K3_000328"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144238.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41170..41622)
                     /locus_tag="ACG8K3_000329"
     CDS             complement(41170..41622)
                     /locus_tag="ACG8K3_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9144239.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41757..42362)
                     /locus_tag="ACG8K3_000330"
     CDS             complement(41757..42362)
                     /locus_tag="ACG8K3_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9144240.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42812..43432)
                     /locus_tag="ACG8K3_000331"
     CDS             complement(42812..43432)
                     /locus_tag="ACG8K3_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9144241.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43434..44303)
                     /locus_tag="ACG8K3_000332"
     CDS             complement(43434..44303)
                     /locus_tag="ACG8K3_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9144242.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44326..44733)
                     /locus_tag="ACG8K3_000333"
     CDS             complement(44326..44733)
                     /locus_tag="ACG8K3_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9144243.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44752..46041)
                     /locus_tag="ACG8K3_000334"
     CDS             complement(44752..46041)
                     /locus_tag="ACG8K3_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9144244.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46068..46370)
                     /locus_tag="ACG8K3_000335"
     CDS             complement(46068..46370)
                     /locus_tag="ACG8K3_000335"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9144245.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46395..48488)
                     /locus_tag="ACG8K3_000336"
     CDS             complement(46395..48488)
                     /locus_tag="ACG8K3_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9144246.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(48977..49741)
                     /locus_tag="ACG8K3_000337"
     CDS             complement(48977..49741)
                     /locus_tag="ACG8K3_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP9144247.1"
                     /translation="MKKINYLAIILLVGLCLFALPPQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49852..50721)
                     /gene="mdcB"
                     /locus_tag="ACG8K3_000338"
     CDS             complement(49852..50721)
                     /gene="mdcB"
                     /locus_tag="ACG8K3_000338"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP9144248.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50746..51822)
                     /locus_tag="ACG8K3_000339"
     CDS             complement(50746..51822)
                     /locus_tag="ACG8K3_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9144249.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51846..52439)
                     /locus_tag="ACG8K3_000340"
     CDS             complement(51846..52439)
                     /locus_tag="ACG8K3_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP9144250.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52443..54119)
                     /gene="mdcD"
                     /locus_tag="ACG8K3_000341"
     CDS             complement(52443..54119)
                     /gene="mdcD"
                     /locus_tag="ACG8K3_000341"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP9144251.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54109..54408)
                     /locus_tag="ACG8K3_000342"
     CDS             complement(54109..54408)
                     /locus_tag="ACG8K3_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP9144252.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54510..56156)
                     /gene="mdcA"
                     /locus_tag="ACG8K3_000343"
     CDS             complement(54510..56156)
                     /gene="mdcA"
                     /locus_tag="ACG8K3_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP9144253.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56163..57074)
                     /locus_tag="ACG8K3_000344"
     CDS             complement(56163..57074)
                     /locus_tag="ACG8K3_000344"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9144254.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57194..58051
                     /locus_tag="ACG8K3_000345"
     CDS             57194..58051
                     /locus_tag="ACG8K3_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9144255.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT        16351 a        12448 c        10030 g        19417 t
ORIGIN      
        1 agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt
       61 ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc
      121 ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa
      181 gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat
      241 gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg
      301 atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg
      361 attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc
      421 caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag
      481 cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg
      541 aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta
      601 gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa
      661 ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta
      721 gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct
      781 gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt
      841 gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga
      901 agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt
      961 gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc
     1021 cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa
     1081 aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact
     1141 ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc
     1201 tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt
     1261 attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt
     1321 ctgatcaatt tgcttttagc aatatcgatc agttgtacga tgattttttt ccaagcggat
     1381 tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg
     1441 gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt
     1501 ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat
     1561 tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact
     1621 aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca
     1681 tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa
     1741 ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg
     1801 atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac
     1861 gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct
     1921 ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat ttgtctagct
     1981 gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg
     2041 aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta
     2101 gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct
     2161 ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc
     2221 aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt
     2281 cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt
     2341 ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca
     2401 cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt
     2461 gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga aggataataa
     2521 aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag
     2581 gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat
     2641 tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg
     2701 gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct
     2761 tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca
     2821 tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa
     2881 gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata
     2941 tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca
     3001 actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc
     3061 tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc
     3121 cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta
     3181 ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat
     3241 gatcatgact cataatctct cctccttcta aacagctatt tctaataaag aaacataact
     3301 tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata
     3361 ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt
     3421 aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc
     3481 ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt
     3541 gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg
     3601 ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg
     3661 ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag
     3721 acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga
     3781 tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc
     3841 taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt
     3901 gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc
     3961 tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca
     4021 tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc
     4081 agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga
     4141 gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca
     4201 ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat
     4261 agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg
     4321 atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga
     4381 aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag
     4441 ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat
     4501 gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa
     4561 catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc
     4621 gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat
     4681 catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat
     4741 cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc
     4801 ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg
     4861 ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag
     4921 ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt
     4981 ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa
     5041 gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt
     5101 tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac
     5161 gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg
     5221 taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg
     5281 ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc
     5341 atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc
     5401 tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag
     5461 ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac
     5521 tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac
     5581 actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc
     5641 atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg cttccttaat
     5701 agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact
     5761 taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga
     5821 aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg
     5881 atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga caatattgct
     5941 tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt
     6001 ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc
     6061 cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt
     6121 cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag
     6181 ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc
     6241 catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca
     6301 tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca
     6361 caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac
     6421 aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct tcttgaagtt
     6481 tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa cgaacagtgt
     6541 agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca ttactgggat
     6601 ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag
     6661 ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc
     6721 aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag tattaggcag
     6781 cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga gataagtagg
     6841 ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca agaattagat
     6901 ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat ttctttttgc
     6961 ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa taactgacag
     7021 aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat ggctctgtcg
     7081 tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct gccgaatgca
     7141 aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct tcaaaatcat
     7201 tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc gctgtgatat
     7261 ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt aattgctgat
     7321 tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct tccgcttttg
     7381 ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact ttatttttaa
     7441 gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt tttgcagctt
     7501 ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga tcgatttggt
     7561 cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt tctcctgaat
     7621 tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca gcaccagaag
     7681 agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca aaagctgtct
     7741 gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc attctttttt
     7801 tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag caaaaatgtt
     7861 tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct tttatttttt
     7921 tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt tgactgctgg
     7981 aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca tatatagtgg
     8041 tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg aagctagcaa
     8101 ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg taaaagtaat
     8161 ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag caaaaagaac
     8221 tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa tgaagatgat
     8281 cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg tcagccagac
     8341 ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca acgtcacagg
     8401 tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat ttaactgatg
     8461 atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat
     8521 caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac caccgaaaac
     8581 agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg tttgagattt
     8641 tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag caatatgtct
     8701 tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct ccggtaccgt
     8761 gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga tattaaacgc
     8821 gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt attttttcat
     8881 cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc
     8941 atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatattcga tcacaatttc
     9001 ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg gaacatctac
     9061 ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa taaaccatac
     9121 aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca aacaagtatc
     9181 taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc attttcttta
     9241 tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga ataaaaacgg
     9301 accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc aagtaattct
     9361 tgtctttttt tcaatttttc atcttcatat tcaaaagatt tatttacttt tttgtataga
     9421 tttcgattgt tccacctagt caatacaaat gaggtaatca ggaaactgaa aaaaatcaat
     9481 acaaaaaagc cgattaagaa atacagcatt ttcttttctg ataaaaaagc gtacaaggaa
     9541 aaatccttgg ctctttctgg tctcgttaat gggaaaaaga cccatataaa aggaagaaca
     9601 acgatcatcc atcctaaaag agccactaac gtttgccaaa tctttaacca gtaattacct
     9661 tttgaaaaat acttatcttt caccaaaatt gatgcaactt cttctcttgt tacttttttc
     9721 atatcctcac ctcacgtttt tttgttacga cgtaggagtt taccatacat ttcatttctt
     9781 ttcacataaa gtgttcctaa atttttccta agaaaaagaa caaaacattt ctcatcagca
     9841 aatgaaaaat attttgttct ttttatcagt tgttcttttt attttttatc tcttaattat
     9901 gttgctcttt ccaatacgac aatgcttttc attcctctag atatcgccgt gtacatttgt
     9961 ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat ataagattac atgatcaaat
    10021 tccagtcctt ttgctagact caaaggaatg atttggcagt tttctaatcc atatgaattt
    10081 tttttaatcg attcttgtag cgtttgggtt tcctcttctg tttgggtgat aactgccgct
    10141 ttttctcctt ttagagaaac caaaatctca gctagtattt tcaacaattc ttcagaggat
    10201 cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga tcgatacgat gtcgatcttc
    10261 tcttgatcca cagcataggt tttgaaaagc tctgtgattg ctccgctaga acggtaacta
    10321 tttctaagat cataccgatg gatcggcaga tggtagtcgt cgaaacaacg cataatatca
    10381 gcaaaagtag tggaagaatt aaagattgcc tgattctcgt cacctactaa agtaaagtga
    10441 gtttttggaa ataattcgat caatagacct aattgtgcca gggtataatc ttgtacttca
    10501 tcaatcaata gatagcgaaa tgctggtaca tttacctttt caacaagtaa atgacgaata
    10561 gctaatataa tgactgcttc atcaacagta cgtgtgggtt gccaagcata agctgagcca
    10621 tatcgttttt cataaattct ttcaaacaac tgatgctcat ccacccatgc catttcttcg
    10681 atttgtctgg ttatttttcg atattttttt tgtaaaagtt tccttgcata agctacaata
    10741 ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct atcttctgtc
    10801 aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag atgattcttc
    10861 cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt aggatgagca
    10921 ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt tccattttta
    10981 gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg ttttagatta
    11041 tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc tcccgtgata
    11101 cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga aaccagctgt
    11161 atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt cgaaatatag
    11221 tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat cgtatctcga
    11281 tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc acttcctgct
    11341 acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt ttgttccgat
    11401 tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa gacttctaat
    11461 aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg atctctttcc
    11521 aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg aaccgtataa
    11581 gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa
    11641 acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa ggattctttt
    11701 ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt caataaacgt
    11761 tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg atcgatttct
    11821 ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact gtcaaaattc
    11881 aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt tgccgtatcc
    11941 atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt cttcagatat
    12001 gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt ttaaagtggt
    12061 ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg agtatcattt
    12121 gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg caccatcaag
    12181 gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc tacgtaatcc
    12241 gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc ggtcccgaag
    12301 ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt cttctgctgt
    12361 tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat gccattgtac
    12421 ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt ttactggatc
    12481 tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat cgaagatcgt
    12541 caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac cttttgcccc
    12601 aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact tcagtaaaat
    12661 atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa tgacttcaaa
    12721 agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg cagtcattcc
    12781 aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa ttccggaaat
    12841 cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc
    12901 ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa tatcaggaat
    12961 attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt cgattccagc
    13021 ggtcactgct agctggttat caggacgaac accaatggat aaaataacta aatcagcagg
    13081 gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc
    13141 atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa aagctgccat
    13201 ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga cttctaaacc
    13261 tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa cgatcacggc
    13321 acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta catttctaat
    13381 agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg gttttgcacc
    13441 aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat gtacgacttc
    13501 taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca catctaaaca
    13561 aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt cactgatttc
    13621 tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa
    13681 aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag acattccacc
    13741 tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt cgatcaaacc
    13801 attgattcat gccaccacgt acattcacta catcataacc agatttcttt aattctttag
    13861 ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt tctttgtctc
    13921 catcatatct gtttatactt tgtaatggaa catttttagc tccttttata tgcccgcccc
    13981 ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc ttttccttta
    14041 attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc tgcacctcat
    14101 ttacattttt ataaatacct catggggtat attaaacagg agctttatta atatgtcaac
    14161 caaaatgact cttcattttt ttcactactt tttatatttg cataaaatac cgataagggg
    14221 tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat aaaaatatcc
    14281 tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg
    14341 aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt attgatcgcg
    14401 taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct gatagtaatc
    14461 cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa aagtaaagga
    14521 atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc agaaacaggg
    14581 aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt atctgtaaat
    14641 ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga tttgcaagga
    14701 gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga aagttcttta
    14761 cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact gcaatcccaa
    14821 ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat cagcttcaag
    14881 aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca aaggttgtga
    14941 aaacgctgtc ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt
    15001 cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt
    15061 gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag ctcctcatat
    15121 tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt aaggaagaga
    15181 ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggatttctac
    15241 atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg tttaaggaag
    15301 aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagctgct
    15361 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaccaa
    15421 gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa aacaacttct
    15481 cctgtttttg gttcggttgg cttattatcg agaagtagct actgggagtc tatttatcaa
    15541 aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca tctttcggta
    15601 ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg caaaaatgac
    15661 tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc caacatagat
    15721 gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat ttttagatag
    15781 cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac caaacaacag
    15841 ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg tcaatttatc
    15901 tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc gagcaatgac
    15961 cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta tataaaatga
    16021 ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata tcgataaaag
    16081 attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt tatcgcccta
    16141 cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac caatgatttc
    16201 actgttttta tttttcttta cagacactga tattatgagc taatttgaga taatcacttg
    16261 ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt tactagtact
    16321 ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt tttataatcg
    16381 ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga acgcaccaga
    16441 cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc aatcgtatct
    16501 tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa tatccgatca
    16561 ccttttgcaa agagctgacg ttctttttta ttctccttcc tatttgtaac tgctgtctta
    16621 ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg ctctgcttga
    16681 tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg gctttctcct
    16741 gtttcttcca acccgcaact gatatttagg acgaagtgct tcccgatcga aagccatagc
    16801 agccggcaca aaatcttcat gatctccggc gaatttcttg atttctccat aatgggtcgt
    16861 ggcaatgatc aatgcccctt gctcatacat agattccatg attgcgatcg ctagtgcagc
    16921 cccttcattt ggctctgttc cactgccaat ctcatctaaa agtaccaacg tattcctttt
    16981 tatctttttc aaaatagcag cgatgttttg catatgacca gaaaatgtac tcaacgcatt
    17041 ttctaaattt tggtggtcac cgacatcgac gaaaatctca tcaaagacag caagctctgt
    17101 cccttctttt gccggaactt gcataccgaa catcgccatc aaagtcaata aaccgactgt
    17161 cttcaacacg actgttttgc ctccggcatt tgctccggtg ataatcaagc ctcgatagtc
    17221 tttaccgatt tcaacatcca atggtaccgc atgctcaggt aaaaatggat gtctgccttg
    17281 cttgataatg atatgttccg atttattgat tctaggtgtg cttccattga tttctcggct
    17341 aaattttgcc cgagcaaaga tgatatctaa caccgtgatc gtttcgatca ataaatcgat
    17401 cacttcttcg ttttctgcga tagcaccagt caatgccgcc aggacttgat attcttcact
    17461 gatttcttca gcttttaaca actgatagtg ttcattcaat ttagaaatca cagttggttc
    17521 aataaaaacc gtcgttcttt tatttgattc atcgataatc gttccatcta ttttattttt
    17581 atagctggct ttgataggaa tcgtatagta ttcgcctttt tgaacaatca ttgcttcttg
    17641 gatcatttct ttgtttttcg gatgacgcaa gaatttcagt aattttgact ggatttcttt
    17701 ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt cgtcactgac
    17761 tttattattt tttattttct ggtcaatcaa ctcttctaca tttatcaaat caggaagatg
    17821 tttgctataa gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa actttgtgat
    17881 catccgactg cttctaagaa aatccgcata ttcgatcaaa tcggttggct gcagaatcaa
    17941 cccttttttg acttgttctg tcagtgcatc gatcctcggc aaccccataa atggaacatg
    18001 ctgactgctt tctaagatca agcgtgcttc tcttgtttct tcttgacgat ccaagacagt
    18061 ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt ttgctatagt ttccaattgc
    18121 tcttgcttgg atttcctttt tgatccgatc aaattgtaat tgactaatca tttcgttgtc
    18181 catattctct ccttttcttg aagagatacc tgtcccaaaa taaataaggg cctagagata
    18241 aataaaagga tagaacacag cttaaaaaag tcatgtccta tccttattag aatacaaaga
    18301 tattgttttc ctgacaggta ccgtttcata agcacaccaa ataaactttg tttattggct
    18361 attcgactta ctgattagat accagctaca ctcacaaaac ataccccatt cttctaggtc
    18421 ttatttattt tccacttaac tatagcaaga cacattttgg ttgtcaaact ttagtatgtt
    18481 ttatagattt acaccattaa tgaatagaca cctaccgcga taattgctcc gatcattgga
    18541 cctgcgattg gtacccatga atatgcccaa tctgaagttc ctttattagc tattggcaat
    18601 accgcatgtg ctaatcgagg accaaagtcc cgagcagggt tgattgcata tcctgttggc
    18661 cctcctaatg aaagaccgat ggctaagatc aatccaccta cagcgaatac gttcgttcca
    18721 ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa tgtaccaatt
    18781 aattctgtga tcacatttgc tggataattc cgtatagcag gacctgttgc aaacgttcct
    18841 aaaatcgcgc tttcatcttt cgttgcattc caatgtggca aataagaaag ccaaacaaga
    18901 atggcccctg caaaagctcc taaaacttgc gcaatgatga aaggaacgac aagattccaa
    18961 ctaattgccc ctgtcatcgc catagctaga gagactgcag gattcaaatg agctgggccc
    19021 atgaatccag aaatatagac agccattgtt acagcgagtc cccaaccaaa tgcgatgaca
    19081 atccagccag aagcctgtgc tttacttttg tttaaattca cagccgcaca cacaccatct
    19141 ccaagcaaaa ctaggatcag tgtcccaata aactccccca tgatttgagt catatcactg
    19201 ttcatttatt tctccccctt tagttcccgc agatctgatt ctgcaatcaa tgcttctaaa
    19261 cgtttagtct gttgcgcctt ctcctcttct gtccattcaa aatatccagc aattgcatcg
    19321 atcacaggtt gcttgatctc atcaagctga tctcgttcaa acaacagatg atttgtacga
    19381 cgaatgagat aatcgcctgg ggccaaaacc atttcttctt ctaaaccata acgtaaccga
    19441 gctgactcag cgagactcaa accaggataa ggtgccattt cttttgctaa ttcaaagata
    19501 cgtcgggcat tagtcccgta aaaatcagcg atataagtag catcttcttc ctctaaaccg
    19561 gctgctactc cttcttttgc taattctgtc actgtttctt ctaatttagt tggatcgaag
    19621 tttccacctg aaatctgata ttttttagag tcgatttctt tcgtctctat tccgtattct
    19681 tcttttaata attggcgtat caatcgcaaa gctccttctg ccatcttacg gtaatcggtg
    19741 attttcccac cagataaagt cagcaaacca tctggttctc tttctaaaga actacctctg
    19801 gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa cacgtcttct
    19861 acttcagcac gagaaacttt gttttcctta tattcactta ctgtatcgac aactttattg
    19921 aagctcttgt ctgaaatcga tccattatct ccaccattgt aatcagaacc agaattacca
    19981 atcaacagtg gacgaagtcc tgcccagctt gcttcgatat cattcaatgt gatatttgct
    20041 tctggatagc gatgattgat cacatccaat agataatcta catcttcttg tgtgactttc
    20101 ggatccgtaa aatctccttg gtaatccgta tctgtcgtac caaagtaagt cttgttttct
    20161 cttggaatag caaaaaccat ccgcccatct tgctttcctg tatcgaagta tgtgggttgc
    20221 ggtacaggca gttttttcgc atctacaact aaatggatcc ctttagttgg acgcatttta
    20281 ggagagactg gacgcgtaaa attcaagttt cgtactttat ctacccaagg accactcgta
    20341 ttgatcacta attttgcttt gatttcgatc acttcatctg tcagcaggtc acgagctttg
    20401 acgccaacga tttgatcgcc atcatataaa aagccaaccg ctttcatttt acttactaga
    20461 taagccccat cttcggcagc ctttttgata ttatcaatca ctaaacgggc atcattgttg
    20521 cggaaatcta gatagacacc cgcgcctttt agcccttctt ttttcaaaaa cggttctctt
    20581 tccaatactt cttctggtgt cagcgtataa ttctcatatt tggttcctgt cacatttgcc
    20641 agtttgtcat aaaggtccat tgctactttg accgagaaca tattgaaggt tgttgctcct
    20701 tcgccctcat agattggcaa aagcattgga tctggttttg ggatgtgtgg ggcaatactt
    20761 tgtacgactg cacgttcacc aactgtgtca gctactactt ccacatcaaa tgtcttcaga
    20821 taacgaatac cgccatgaac aagtttggtc gagcgagagg aagtcccttc tgcaaaatcc
    20881 tgcatttcga tcaatcctgt tttgattcct gatgctgctg actggactgc cacaccggca
    20941 ccagtgattc cgccaccgat gatcaacaga tccaattctt cttgctgcat tttttgaatg
    21001 ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct ccttattcaa atgcttgggt
    21061 agcttttact gcttttttcc aaccgttgta aagtttttct cggcgctctt cctccatttg
    21121 aacttcaaat agcttgccag cttcataaaa ttcgcggatc tcgtctgtat ctttccaata
    21181 accgacagct aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca agttttctgc
    21241 tcgttggatc ggtacattca agatatctgt ttggaattgc atcagatatt cattgtttgc
    21301 tgcaccgcca tctactttca atacaggaat cttgattcca gtgtcatctt gcatcgtatc
    21361 gatgatatct ctcacttgat aagcaatcga ctgtaaagta gctttaatga tatcttcttt
    21421 tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc aataaggagc
    21481 acctagccca acgaatgcag gaacaacata aacctcatct tcgcttgtag agcgtttcgc
    21541 tgcgtcttct gaatcgctag ctttttgcaa catttgaagt ccatctctca gccattgaat
    21601 cgaagaacca gccacaaaaa tacttccttc taaagcatag taaacctttc catttatgcc
    21661 gtaaccaatt gtcgtcaata aattattttt ggataattgc ggtttttcac cggtattcat
    21721 gacaataaat gaacctgtac cataagtatt cttgaccatg cctggttcga atgccatttg
    21781 tccgaataat gcggcttgct ggtctcctgc catccctgaa attggtgctt cgcttccata
    21841 aaaatgataa ccttgcgtat aaccataaac ttctgagtta gaagtgactt ttggtaacat
    21901 cactcgagga atatttagta aatctaaaat ctcctgatcc caatctagct gatggatatt
    21961 gaacaacatc gtccgactgg cattcgagta atcagtaaca tgtgcttgtc catcggtcag
    22021 tttccatact aaccaagaat cgatcgttcc aaacatcaat tcgccttttt cagctcgttc
    22081 ttgcgcgcct tctacatgat caagaatcca gcgtatcttc gttgctgaga aataggcatc
    22141 gatcactaac cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt ttaagttatc
    22201 tgcaattcca gctgactgtc tggattgcca aacaactgca tgatagattg gacgtcctgt
    22261 atctttttcc catattactg ttgtttcacg ctggttcgtt atgccaatcc cagcaatctg
    22321 atttggtttg atccctgatt cgatgaacgc tccggcaata actgactgta cagaattcca
    22381 gatttcattt gcatcatgtt ccacccaact ttcttgcggg aaatattgcg tgaactcttt
    22441 ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg agctagttgt
    22501 accttggtca atcgccatga tatacgttga ttctgtcatt gtgattccct ccattatatg
    22561 caaacgcttt ctttaactac tcttatcata ctactgtttt aaaaaaagaa actgtcaaaa
    22621 ttttattctt tttttctttt tttgacagtt ttttattatg aagctatttt ttaatcaatt
    22681 cttttctttt ttcagcgtct ctattcttct tttcaatcga ataatgtcgt aaggtgacgc
    22741 aaattctgac agataatatt cacactgttt tttgtttctc tggcaaaatg tgatcaccaa
    22801 atcatattct ttaccttctt cataacttcc aatctcaata ccgccgattc ccctcagttc
    22861 tctgatcaga tattgttgaa aagcaatacg ataaatcggc agactttcta aatcgattcc
    22921 aatcactact ggttttgcaa tataaaaatc gatcatcaaa agaacattgg catactggat
    22981 catcaggtaa ctaagctcac tatcttcagc tcttttcctc ggcaattgct gcaccgctgt
    23041 ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg agcattttct gctgctgttc
    23101 caaaagccga tcacgctcat agacttcgat ctttccaaca aagaaaaacc attcattgtt
    23161 gatctgggaa atttggtagc ctactgcctt ttcttctttg attttcaaac gacgaggacg
    23221 ataatgccat aacatcgttt cccgaatgta agtatctaaa acagcggttg gtaacttttt
    23281 gctccgtgtc aaatcatact gataaaatat ttcttcgtct actatcgaaa aagaaacaaa
    23341 aaagctgtaa aacatcattg tttcgtatac attcagttct gctgctgttc gactaagatg
    23401 catcacgatg attggatcga ttgcttgata aagccggtcc gactgataga gtaatttctt
    23461 ttctttcaaa gttgcgtacg tacttttttc attcaatagt ctttttttcg taatcccgag
    23521 ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata cacggtccaa
    23581 tcctagaatg aacggtcttt tttctggtgt attttgcaaa aaaagcggtc gctgatcttc
    23641 cgggatatat tggaatagtt cataataaaa atatcgtatc tgtagctctt ctccgtataa
    23701 ttgaccgttt ttgatttgta atccaaattc ctctaagagc tggttcaatt ctttgatctt
    23761 tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct aatcgaacga tggatacttg
    23821 ctgatgttct aataaataga cgagcatctt aaacttcaag ctttcctgca ataaaaacaa
    23881 tagaattttt tccaaactta catcttcgtc caacacaagc agcgcctgat actcatttcg
    23941 ctgtacttct actttttttt ccatcattcg gccaatcaat tcaatatctt ccaaatattg
    24001 atcaaacgag gcttttgaca gttggacatg ctctctcaaa ccattgatag aggctgttcc
    24061 cccttgcaga acaaggtttc gcaaaatcag gatctgtaac tgctcttttc tttctaataa
    24121 tgcttcaatc ttcataggca gtattcgcca cctttgacca caccatatct ggcacacggt
    24181 attctggctg gaaaaatagc tcatgttctt ctaactgttc aaacgtccat ccttcttcta
    24241 ctgcaattgt cagttggtca agaaggtgga acatctcttt tttgctttgg agctgtgctc
    24301 caagaatctt tttttcagaa tcaaatagaa atttgagttt aactttgctt tgatttattg
    24361 ctgtaagctt gtagtctacg tatcctgttt caattttttg gggataaaaa atgctctctt
    24421 cttctgtcaa acctacgctt cctaagaaat agccaaacca ctgattgccg actggacgat
    24481 aggttccctg atcctttgtc tgtgagccac taattgtctt gctgacattt cgtgctgtac
    24541 ggacagcgtt atttactaga gaaacataca ttttttcatt tgttggacga aaagttactt
    24601 ggatcgcatc gccaatcgca taaacgtctt tttgagacgt ttgcaagtag tcatctaccc
    24661 agatcgttcc atcatcattc aatgttaatt gttcttccca catctgattg tcgggacgag
    24721 aatgattagc cagcaaaaca agatcatagt tctcctttct tttttccgtc tctacaatag
    24781 cggtttcatt ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa agcccttgat
    24841 ttttcatttc ctttactaac ggttctgtca tttcaggatc tagataacga aaaagaattg
    24901 actttcggct ttcgtaaaga tggatttgtt tttttcttga tgccaaccct tcagctattt
    24961 cgattcctgc ttgtcctgca ccaataattg caattttctt tgcattcatg atcttttgct
    25021 gaagagccgt tacgttttgt atctttttaa ctgggtgaat aagcgttgat tctgcagaaa
    25081 tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa tccaattttt
    25141 ttcctcctgc taaaacgact tttttttctt tcaatcctat tactgtcttt tctgtgtaaa
    25201 catggattcg atagcggtca gtcagttctt ctttcgtgat ccaagaaagt tgtttttcac
    25261 tggtaacctt tccttttaat atcaaattga acccgctggg aatccatcct aacttcgatt
    25321 gcttttcaat caaatatatt tcacttgttg gcatcaattg gcgaagataa atagccgcat
    25381 gaatccctgc aaacgacccg ccgacaatta caatgttcat ctgcattcct ctttcttata
    25441 aggtgttcta ctgctaactt ctcaatcaga aaacacagta ttcataactc gctttcaacc
    25501 ttatgataca tagcgatgca gcagaatcaa ccctctccat cataccgtgt tctaggtctg
    25561 cttctcaata atgtactagt ggacctaatt gaagagtatc atttctgaag agatgtttca
    25621 aaaaatgtac tcttataaaa gaaaaaaact cgccttttac gtacctctgt ccaaatagga
    25681 aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta gaatagacac
    25741 atcatttatt tttaattctt taatgcttca acaactgctt cgcctaattg atgagcagat
    25801 tgaggatttt gccctgtaag aagccgccca tcaatttgaa ccacttctga aaaagctgct
    25861 ccagactgat agtttgctcc tttttctttc aaagcatctt ctgctaagaa tggtacttca
    25921 ttcgttgtac catttgcttc ctcttcttca ttactgaacc cagtcactgt acgtccagct
    25981 aaaatagatt ttccagactc atctgtaact ggcaacaatc ctacaacacc gtgacaaaca
    26041 gctgagacca gcccttcatt tcgatagatt tgtgaagcga ctttcccaag atctattgct
    26101 tctggaaaat cccacattgt tccgtggcca ccagcataat aaaccgctgc ataatcattt
    26161 gcatccacat catcaggacg taaggtattt cccaaggccc gatttctgaa atcttcatct
    26221 tcataatagg cccaatcgat ttcatccata ttcaaaacac tttgcggttc taaaggtaca
    26281 tatccgcctt taggactgac aaaatctgca tcgatcccat tttttactaa tacatcataa
    26341 aaatgagtca gttcacttaa ccacaaacct gtaggacgct cgatcttttc gtacttttct
    26401 acatttgtta atacgatcaa tactttcatt ttcccatcct ctttctataa aaattgcgtt
    26461 attgataacg tgctttcaag aaattatata atgggcttgg caaataaagc tctttatatg
    26521 cttcttcaaa aggtttcgtt acaaattcat cctcacggtt ttccaataat ttctctgccc
    26581 agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat ttcaatacct
    26641 gctctacatc ttcgcgattg cggatcccac cgacaccaac taaagggatc tttccttgaa
    26701 tcgtgttatg gaccacttca agaatcgatt tatcgttgta tttatcagaa cgagctattc
    26761 gatcatacat atttaatgaa acatgcagat aatctagttt tgactcacgt aatcgttcaa
    26821 gaagccagat cgtgtcttca aaacggatac ctggtgtttc atattcttct ggtgagaaac
    26881 gataccctac gatgaatggt tttttagcat attgttcaac cgctgagaaa accgttgtta
    26941 ataattcttc aataaaacga taacgatttt ccaagctacc gccccattga tcctctcgtc
    27001 tattcgaatg cggagagaaa aattgttgga tcaaatacgt atttgctcca tgcaactcga
    27061 ttccatcaaa tcctgattca atcgctctac gtgtggcttt gccaaatgct tggattgttt
    27121 caacgatttc ttcttcactc attgctcgtg gtgtttccgc atcaggacgt tttgctgcaa
    27181 ctgcacttgc agatacaggc tgttctccag ataaagttgc tcgacttgtc attcgtccac
    27241 cgtggaaaat ctgaataatc gcttttgcgc cttctccttg gattgctttc gctagctcac
    27301 ttaatcgagg aatcatttca tcatgagcga tgctgagttc acctggccag ccttttccga
    27361 tatcttggac atttgcagca ccagtaataa cagcaccaag acctttagaa cgttgtttat
    27421 aataagtgat ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt gtcgtcatag
    27481 gcgacagcat caatcggttc ttcaattgaa ctcctgaagg taaagtaatt gattgatcaa
    27541 attgtgacat tgtcattcct cccataattc gctctgatta acctatcgag aatcatctat
    27601 cattaaacca ctcgattagt tgtacatgca actaatttgc ttaaaaaatt ttattttttt
    27661 tgtaacttat ctgttgcatc attcattgtc tgggctaatt ctttacttgc ttcatttccc
    27721 aaaactgctt tgtatgactg atgaagttct tcccagctac catcgacttc cttagcaaat
    27781 tggcgtcctt gttctgtaag ataaactaat gacaagcgac cttcgctaac tgttttgacg
    27841 tatcctttat attctaattt ttcaacaaaa cgagcaatcg ttgatggggc ggaatagagc
    27901 atttgtgcaa tatctttttg tggtattcca tctttttctt ttaatatcaa taaaataaaa
    27961 ccataagttg gtgccagacc tgtccttcta aacgcttcat cagctaattt attcaacatg
    28021 cgatctagct ttttcacgct aaagaaaaga cattcatcta acgctttttc aattggcttt
    28081 tccatttcat cccctccatt ggcgtacata tcatagtatg atttttattt tttgtcaaaa
    28141 aacacggaat aatgtccgcc tcctctgtct agatttgaaa aaaagaagta gccatcttca
    28201 aagaatttcg gcttgttttc gacaaaactc cttctggaac accgtgaatc agaggttgtg
    28261 aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg aaaatggctt tccacatttt
    28321 tgcataaatt tggcttaata ccgaaaaact ggcttttgaa caccgtttat atcagaggtt
    28381 gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
    28441 tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt attgagaggt
    28501 cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
    28561 ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt ttattcgtaa
    28621 agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt ataggatttc
    28681 gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta accgtttctt
    28741 tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt ttgacatttg
    28801 tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa ataaatctac
    28861 tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt caatgcggta
    28921 atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc cgttttcagc
    28981 tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc tcaaaccctt
    29041 aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa ttcctccagt
    29101 tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg cactagacat
    29161 gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt catcacctac
    29221 ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg ctggataata
    29281 tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa ttgttttttg
    29341 aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca tacaaccgac
    29401 ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg ctataaactg
    29461 atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt caattactcc
    29521 tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca tccaatattt
    29581 tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa actgaataag
    29641 ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt cataatggga
    29701 aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt gatcataaaa
    29761 aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc ttgtccatgc
    29821 gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt cttctttaaa
    29881 gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt tgggatcctg
    29941 cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt tttctttagg
    30001 taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat ctacatccca
    30061 tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt ctggaaattt
    30121 tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt cttatgacta
    30181 gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt cggatcatat
    30241 ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt acgtactttt
    30301 tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt atcaataccg
    30361 atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg tttcgttgga
    30421 aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt tggaatgatc
    30481 gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc agataaatca
    30541 attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc ttcttcgatt
    30601 tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc taagaaataa
    30661 ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa aatacttctt
    30721 gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa tatagctcca
    30781 catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata aacagcaggt
    30841 tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt tactttctta
    30901 tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac ggcagtcata
    30961 catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat tactggtaca
    31021 agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat aacagcacca
    31081 gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag ctgacttgca
    31141 taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat gatacaaatg
    31201 aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt aggctcaata
    31261 taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata ccttgctaga
    31321 atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact gacaatcgta
    31381 ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt gaactttttt
    31441 gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc tgctgcattc
    31501 aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca taaggcgagt
    31561 actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa aacagcggcg
    31621 atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt tccactagat
    31681 gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt tactgcattt
    31741 cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc ttctagttgc
    31801 tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa ccttgttgca
    31861 caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat atctttcgct
    31921 aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc aatccttctt
    31981 acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc ccttctaata
    32041 gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc actcactgct
    32101 tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa aacacgcttg
    32161 atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat ttttgtttct
    32221 acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag cataactagg
    32281 tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat ttttatttga
    32341 ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc aactatggtc
    32401 agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag aacactaggt
    32461 agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa aattgcatca
    32521 tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat ccggcattct
    32581 ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa aatactaatt
    32641 ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa cgcttcggca
    32701 agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa atgtattggg
    32761 aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt ttgtacaaaa
    32821 tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat atgggttagc
    32881 aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg aataatcgtt
    32941 tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt aatcatcaaa
    33001 taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga aaaagaatag
    33061 ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa taatgtatac
    33121 ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag ataccgagaa
    33181 aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac ctcttttaat
    33241 gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg aggcaacctt
    33301 gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt tgaattttct
    33361 tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt aggattgcgc
    33421 tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga aagaaagaaa
    33481 atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta cgtcacaata
    33541 gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc ttgtatgatt
    33601 tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt atttgactgg
    33661 ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg gatcgttttc
    33721 ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt cttatttctt
    33781 aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca cctcctcttt
    33841 gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga ggtttaaaaa
    33901 tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa tttgtttata
    33961 atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat tttgtctatc
    34021 cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata cgatatcgat
    34081 tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct cagcaatttc
    34141 ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat tcaattcatc
    34201 tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt cataacggtc
    34261 tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac tccaaggttc
    34321 gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag tatctacgcg
    34381 catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa tataatctgc
    34441 cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac agtcttcttc
    34501 cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat cggagaaaaa
    34561 ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac agtcatatgc
    34621 ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg gatcgatttt
    34681 ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg cttgataaat
    34741 cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga atgtaaccaa
    34801 tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg tcatccaata
    34861 tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa aaaagtcgat
    34921 taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg tttcataatg
    34981 actaagagta attactggct cgatatcata tcgatttaat gtattgatta gtctgtcata
    35041 aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata ttcgtgccca
    35101 tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa tatcctcttc
    35161 aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt cgtctaaagc
    35221 aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt tcatgatccc
    35281 gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac cttcacattg
    35341 attagcagct actgctccgc cccataaaaa attttctgga aatatttcct gatccataac
    35401 tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca tcaaaacaag
    35461 atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct tcatttttaa
    35521 tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt taaccatcta
    35581 agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa tcaactttgc
    35641 cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct tcacgtggaa
    35701 tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc tggtcttcat
    35761 atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca tagtctcccc
    35821 agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata taaaaaacag
    35881 aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga ttgactcgaa
    35941 gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc ataggcgtcc
    36001 tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt gtaaatccag
    36061 aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc gcgatatcat
    36121 cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg gtgcctttca
    36181 tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca tcgtcatttc
    36241 cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta ttccatcctt
    36301 catcacctaa agatgtttgc catttggata atactttttt cagttcagag atactcaagg
    36361 gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt tcgaattgga
    36421 agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt tcggtagttg
    36481 ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct tgattcattt
    36541 cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc ggttctttgc
    36601 caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc cagaaattca
    36661 tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca ggttgacgct
    36721 tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa gcagaaccac
    36781 tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc caaatataat
    36841 agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga tgttcatcag
    36901 aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt gcttcttcaa
    36961 tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga taattactga
    37021 tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa attgcatcaa
    37081 tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct ccaataccat
    37141 cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa tggtgccacc
    37201 atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc ttaatcattt
    37261 attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca ccttgttatc
    37321 ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt tttgaaagga
    37381 ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt gttcactcag
    37441 ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt ttcgtttttt
    37501 tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca ttcaatatcc
    37561 acatgaatcc cacaaataca aagaacagac catacatgat tggagatact tcttttcctc
    37621 ttttagctgc taacatggta attggataga agataaaacc taatgcaatc ccatctgaaa
    37681 tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca atctctaagt
    37741 ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata atcaatgctg
    37801 gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag cctaacaaga
    37861 agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg ataccagctg
    37921 atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca agcatagctg
    37981 ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc atgaaacctg
    38041 cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca agcaagaatg
    38101 tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt aatgcaacta
    38161 aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt ggaatcaaac
    38221 ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca cctggtacac
    38281 gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa gtagaaccaa
    38341 cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc ccaccttcgc
    38401 tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat ttcaaatctg
    38461 ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa ataaaaatca
    38521 aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt cctaaacaaa
    38581 cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt ggataacggg
    38641 ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca gtaaacactg
    38701 ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag atataagcca
    38761 ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg ttcaattttt
    38821 ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa tccgaacatt
    38881 ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt atcatatact
    38941 aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa cgttccatct
    39001 gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa gaatttcttt
    39061 attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga tttgatttta
    39121 ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt cagattattt
    39181 agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt ttttcctgat
    39241 ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt caaataacct
    39301 tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag acgactcagc
    39361 ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca agatgccatt
    39421 gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa attcatccgt
    39481 aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct gcacaaatac
    39541 ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac tcttaaattc
    39601 ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa agaatcggca
    39661 aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca tcatttgacc
    39721 cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat cacgaaagcg
    39781 caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct aggattgctt
    39841 tcctccacaa acttttttta caaaaaaaaa aacggcaatg aatgccgctc ttcttatttc
    39901 gattctttta taattctgct tctgattcat ctttggttag tgtgtgtcta acactagtta
    39961 cgccaaatgt ttgatcgatt ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg
    40021 cttgttcagc attgatggat tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca
    40081 tcgttctttg ggcttcacgc cagttgagac agcgacagat cgctccttgt tcatcatagt
    40141 agatgatttc ttccggaagt gccggagcat cggaatctgc acctaaaggc aggaatgatt
    40201 cgccaccttg ggcttttcct aaatgcagat ttcctgcgat cttttcgata tcttctccgc
    40261 cgcaaggtac gccaaaagac aaggaaatac tattatagat atctacaagc ggattgattg
    40321 ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac
    40381 cttttttagt cttgaatgtg gtaaatgcct gacgccactg agcaatgact tcgttttggc
    40441 tgaatgtttc ttctgtaagg taatttcttg cttcttttgc tgcctcattc aataattgat
    40501 gatacgtttc ttcactttct tttgttacgt ggttatcgat accatttacg gataagattt
    40561 cgatggttgc ttcaggaaat acttcccaga aattggtatc cactattact tttttcatct
    40621 gttcacacct cttgattaga ataattgctt gatgaataga tagtctgttt ggggatcatc
    40681 tcccatataa aaaacatgtt cgctgtattt gataaatcct tttttacgat aaaaagcgag
    40741 cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct gttttttgct tttcttttgc
    40801 tttttcaata gctgtatcta gtaaaaatgt accgatacct tgatgtttaa atttttttcg
    40861 tacatagatt cgctcgattt ctaaaaaatg atcagccatt tcctcagatt gactcgttcc
    40921 tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata ctcactagga aaaaatcggt
    40981 ttctttatct tttagctctt ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc
    41041 agctaaatgc tccggtgaat tatcttttcc aaatgtatct gtaaatgttt cgatactaat
    41101 ttgttgtagt aagtctacat ctgaaggcgt catttttcga atttcgtatt tattcacggc
    41161 ttcttccttt caatagatcc ttttattgcc ttttttgaca aaattccaat cttcgctaac
    41221 attcttgcat actttattta aaagatattc caattgtttt gcttcttcgt cagaaagacc
    41281 atttaatgcg actgcattgg agtagttgtt ttctcgaatg atgaaaggat agatttctgc
    41341 tcctttttta gtcggataga gtttcttgat ttttttattt tctttatctt ccaaacgttc
    41401 gatcatccca ttggattcta atttcttgat tgctcgggca gtcgttgtac gatctacttt
    41461 gatcatttct gctagttttt cttggatgat tccaggattt tcgcaaatac gtactaaata
    41521 aagatactgt cctcttgtta aatcgacttc tttgaattcg atattgctaa tagaatctag
    41581 cgctcgggca atcattccga tgtctcgaag gatttcagcc atttcatccc acctttattt
    41641 tgtttgtaag actagttcac ctctttttgt tgcaaatgca actattttca ataaaaaaat
    41701 caagttcttt tttaataact agtaaagtat attcttttct tcttttacga aaaagctcaa
    41761 tcacaataga tctggttttt gttttctaaa ataagtaact gtttcttcgt tgttcgcttt
    41821 tctgatagct ttggcaactc ttctatacta aaaaatccca tgttggaagt ttcattattc
    41881 tcaataaact tggcctcttc accgtggatc gcacaagcaa aaacaagttt ataatactga
    41941 aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt
    42001 tctactgtta atcctgtttc ttcatagact tctttacgca cattttcttc aggagacaat
    42061 cctatttcag caaatccacc aggaagtgac cattctttcg tccgtttatc ttcgattagt
    42121 aggaccttgc catctttttt gatataggca cgaacatcta cttttggtgt tggatgtcct
    42181 tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt cttttcctat ttcactgatc
    42241 agttccaatg aaatcgtccg gatctcctca aaacgctctt tatcaaatac atcttttcca
    42301 taatatagac cagcttgaga aagtgcgagt agtcttttgt attgagccaa cttcttgttc
    42361 attctcttcc cttctttttt agcaaatgta tgactatgta atcaagtata gaagactggt
    42421 ttaattacgg caaataaata taatgaaatc tgcaataaat aaaagaatgt taaaaaactc
    42481 gagaacaaaa gacattcgac ttctctgggg ctgtgaataa atggcgttca aaagcttgca
    42541 tctgcggtaa taagccccac caaacgaaaa atatgacgag ctattcttgt ctggctgttc
    42601 ttattaattg aggcaggaca gctttttcac aacctcccca ctaaacggtg ttctcctacc
    42661 aactcctcgg tcacaagtca caatattcat caaacatgag aagctgtttt tttgaaattg
    42721 ctccttgtga ctcggagctg aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa
    42781 gccaattttt cgatattaag ccatttcttt gttacttatt gtcattgatt tctttttcta
    42841 atttatccaa tagtggagtg taatccactt gagtatagtc tgttacatca aattcatata
    42901 gtgttcctaa ctggaatttg tcatattttc ggtcggcgat gatctttttg aatgcttctt
    42961 tcgtaatatg attggtccct ttcgttcgat cttccaatac gtcaccatag tgataatgat
    43021 ccatgatata gctcaaatga cgatctgatt cacgatcacg tttgtaccga cgttgccata
    43081 acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg atatccgtat ctatctgcta
    43141 attcttggag tcgtgctttt tgcttatcac taaaaggata ctcggacaca atcactttct
    43201 ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa aggataaact ttcgtccttt
    43261 cttgttccgc ccgttcttcc aaattgttaa acccgatgga ttctgcatag tattccttga
    43321 tttcatcgag cgtgatggca tacatatctg gaaatcgttc ccgtagttta ttcataagat
    43381 atgtttttcc tgtaccagga ctaccagcca gaagaatgaa atactttttc atctcattta
    43441 tttcctaagt taattggact ttccaagttc ataaacagtc caatcttatg cttgacaatt
    43501 tcttttactg cttcatttgc tggaatcaca ttatggcgaa gagacttgtt gatttctgtt
    43561 tcactaaagg tttctttagc tactttcgtc cagttgacta aaagctccgt tccaacattg
    43621 aatttgcgga tcccgttatt gatcaattca ggataatctt cttctttcac acctgttcct
    43681 ccatggataa ctagggggac atcaactact gcatcgattt ctttcagcaa gggaatgttt
    43741 acttctgttt ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc taatgcatcc
    43801 acgcctgtag cttctacaaa tttcactgcg tcttccggtc ttgtatatac cttgtcattt
    43861 tcagcgacat ggattccttc ttctgtaccg ccgattgttc caagttctcc ttctacggaa
    43921 acaccgtgcg catgggcata ttctactact gcttttgtct tcaggatatt ttctttgaaa
    43981 ggcagatggg acccatcata catgacagaa gtatatccgc tgtcgattgc ttctttgata
    44041 tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat caaccatttc ttcttctgca
    44101 atagctgtca ttacattgac taacacatgc atgccgatat atttagcggt atcgacactt
    44161 gtttgaataa tgatcggcga acccatttct tttgctgcgc ctagcatagc aggaagcatt
    44221 tctaggttat gcgtattaaa agctccaact gtaaaattca actcttctgc tgtttttgtt
    44281 acttctttca atgttgtata catcttttat tgccacctta tctttttatt tgtttccttt
    44341 gatcgttact tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat
    44401 cccttgatca tcaccagcac gagcagcatc tgcacgcttc tggttataga ggctcatcag
    44461 ttttttatac ccatctccga tcgtctgttt attttccaaa ctcttttcta aatattggat
    44521 ggcttgatcg ttcacataca attcagaaaa tttaagtcca acttgttcat aaagtttcgc
    44581 taatttatgc ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa tctctgattg
    44641 aatcttttcg gtcgcttctg gcgatagggc tttttcttct tttgccagaa cttttttcgg
    44701 ttctttttct tttttcttaa aaaatccaaa catcattttc cctactttca attaaaatgc
    44761 tttcaaaact gggcttaaga tccaggcata taagagtgct gcggctactg tatctacatc
    44821 ggttgctgta gcattgatga atcccatcga attaaagatc gttactagca aagcgggcaa
    44881 taatgtaata aagaaaccat gagcaatacc gccaatgatc gctcctcggc gtccgccaac
    44941 tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa
    45001 aatcatcgct aagccgaaca tgggcaaagt aaacatggca ataatcgtac caatcgttgt
    45061 cgtaatgaaa cctaaaatga cagcatttgg gctataagga aagaaaactg gacaatctaa
    45121 ggcagggatc gcatcaggaa ctaatttcat agctatccct cgaaatgccg gcacgatttc
    45181 acctaacagt agacgtacac cagctagcaa tacatacaca cccactacga attggatggc
    45241 ttgtaaaaag gcaaacatga tatagttttg atctcctgat aattcagaag cgaatggttc
    45301 gcctgcaaat aaaacagtga tgatatatag cgggaccatc acgaccatga cagacaaata
    45361 agtatcttgc agaaattcga aatgtgtcgg caacttgata tcttcgatcg actttttatt
    45421 ttcgcctttc tcaccaaaaa gcttagcaac accggcttca aataagtacc cgatcgtaca
    45481 aaaatgacct aaagcaatat catttgatcc agtgatttta cgaatgatcg gttgcgctac
    45541 tgccggcata gcaatggcga aacaagctcc gataaaatca ccgaccaaga tcaatacgat
    45601 ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc ataaaattgc
    45661 ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga gccaaaataa tattgaatac
    45721 aaaaatggct aagaacgtca gtgcaatatc tctacctaaa cccaaatcat tcattgcttg
    45781 accattgata gattcaatag aagggatgat cccttgcata tgaaatcctt cagtaaagat
    45841 tttcccgaaa taagttaaac ttccaacgat gatgctcgaa ccggcactca acacttgaaa
    45901 tcctaggagt gtctttaacg tgccagagat gacttgtcct gctgatttct tctgcagtag
    45961 tagacccaac atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat
    46021 gataaaattg ataacgccca tgtttttacc ctcttttctt tttgtattta gataactccc
    46081 ttatcttgca gaactggggt gattttttct tcaatttcat tttttgaaac aatatttttc
    46141 aaatagatca tcgccgtttt aggatcaact gcgaactttt cgaactgcga acgaaaattt
    46201 tctgctgtga tgatcacatc tggtttcatg gaaactgcgg aagaaatatc acaatggtcc
    46261 agtttggctt ctactcctaa tttgttcaaa acatcctcag cagacatctg tgctgcaaaa
    46321 ctacttccta aacctgcacc gcatacgaat aaaatgttca atttgttcat ctttgttctc
    46381 ccccgtatca tttattattt ctcaactact gaaaaaagca gttgtttgaa atcttcgata
    46441 tttgtgcatt caatcaatcg atccaacttc tcttcgttgt tgatcaattc aattagttct
    46501 ttcatgatgt tcagatgcga gtaggagtct actgctgcca aacaaaaaat aatctttact
    46561 ggatccattt caggattacc gaaatcgatt ggttgctcaa tcgtagctac gctgactcct
    46621 aatttattga ccccatcttc tggtcgggca tgggctagtg ccatgtgttt gccaatcacg
    46681 atatacggtc cgtaatcatt cactgcacga atcatcgctt cgacatagcg ctcttcgaca
    46741 atgttttctt taaccaatgg tctagcagct agttcaatcg attcctccca agtagtggct
    46801 ttttctttga tcatgatatt gctgtccgtc aaaatatctt ttaacatagg ctggatctcc
    46861 cttttgttga tttctaaatt gtttgtttcg aataattttt ctaacttgcc atagatgatt
    46921 cgatctactc ggccaccact ttctttcatg acatctatta tttgagtgaa taactccgtc
    46981 gattcatgac tgttaggctc taaccgctgt agttcttcat ttcttttcaa aaagtcttct
    47041 ataaccaaga tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg cttatttcga
    47101 taattcaatt gaaaggtgct gaaaaccaga ttgacatcca atttatctac taattcgatt
    47161 tcttttgaac ttaaaactgc agtaatctcg atctgaggaa acttttcttt cagattctct
    47221 gctaacagat ttccagtagc catcccatgg ttacagacaa cgactgattt ataaacataa
    47281 ttcaagtctc gcttgattac actggcgatc gtcgaaaagt ggatgacaag aaaagctatc
    47341 tcgtcctcca gtatcttgcc accaattact tcttcgatcg taggaacaaa atgagtgact
    47401 gcgcggtaga ttgggaaata atttgttcgg atgttttctt tcaatggatt catgacttgg
    47461 atatgattct tgattcttac aatcagtgca gccatgtgct tatagagatt ttcgaataaa
    47521 gcttcttcct ttagatggaa aggtacgtgt gtctcgttct ctacaaattg gatcaattgg
    47581 atcgttagta attgagcttg tacccactcg attgaattgg agatatctct agcattgaac
    47641 gtctcaatag tgaaacgaag ataatttctt tctacttgcg ggacattgtc caattgaaat
    47701 tcctcgatca tgtggtcaat aaatgcagaa aagctactgt cttttatttc tgtatttttc
    47761 caattaatgg cagagataaa ttcatgtttc ctgattcgct ggatccagat caatgtaaaa
    47821 agcaggattt gttttttata aatatcatct tcttgtttca acaacttatt ggaatagatc
    47881 tcctgtattt ttttgatttc caccaaagga aaataatgga gaaagatttt ttgagccggt
    47941 gttaattgtg catcttgatt cataaagaag tcggtcattc ccagattctg attgataaaa
    48001 tcatagatca tcgtacggat agatcgttct gaacctttat aaaccagtcc ttgctttcca
    48061 taactgacga tctctatccc ataactgacc agtgttgctc gtaaccgtct catatcttca
    48121 tctaatgtac ttttagagat ttgaaattct tcttcttttt tgtttagatg gatcgggatt
    48181 cggctgtaag caacggctaa aactagatca aaaatacgtt cgcgccgatt caaaaagccg
    48241 tctttcacat cttttatttc agtttctaat cgagcttgct tttccttttc cagcttcaac
    48301 aaaaaacctt tgttgcgaat tgtacggatc tccggaagct ttttactgct caaccagata
    48361 ttcgcttcaa gaacttcctg acggatcgtc cgctgactga cattgaactc acttgctaga
    48421 tgttttgctt ttactggata ttgggctgtc atgattctgt gaagtatttc aattgttctt
    48481 gttttcatag aaatgatctc cttctgattt caagtatatg gttccgctta cataataaaa
    48541 agtagatatt tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca aaaaataagc
    48601 catatgcttt ttcatctcct caacgaaaaa gacatacgcc tattattaca aaaattttgc
    48661 ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacaact
    48721 aaactaaaat agtgcttcct atttttcgtt tggttgttct tattatcgca gatgcaagct
    48781 tttaaacacc atttagtgga gaggttgtga cagagatgtt cagccttgag cgataagaag
    48841 cgattttgaa aaatcgcttc ccatatttta caaaatcgtg ccttattgcc gataggctat
    48901 ctctggcaca ccgtttattc agaaaaagga gctgcgacaa ggcttttgtc acaactccgt
    48961 tcataaatat ttcctgtcaa tccagcataa tcggctgaat aaaatcggct tgccatcttt
    49021 tttctttctc agaaaaatca aaataccata tctgagaaaa atactgtcgt tgtgtcttac
    49081 ttcctgagat caatcgccca tcttttcgat cgactgaata atcacagcat ctgaaatcaa
    49141 ttcggatggc aaaactattt tcgtggatct gtcgataatt tcctacttcc tgaatagtta
    49201 tttttttagt atgatttctt acaccgtctg ctttgttttt ttgcagaact ttctgatgtt
    49261 cggcaaacag cttgtctgta taacagcttc tggctttttc aggagtttct tcttcccaag
    49321 cggattggat cttcatgaat atcttttgga tttccagaaa ctgtgctttc ttttctttag
    49381 ccgttcctga catgcgggac aatatctccg ctttcagcct tttcttttgt cttttattct
    49441 tgacgataga aaaagcagaa acccctccta ctgacaaaac agctaatgca agaaatcccg
    49501 tttgctcata actagaagga cctgtacctg gaccataata cccataagaa tctctgccgt
    49561 aagaacgact gccaaaataa ctgcccccat atgaacgacc accaaatgaa cctctgcccg
    49621 aacttcgacc gctagatgta cctccaccat gaccacccgc gacagcgaat ccttggggcg
    49681 gcagagcaaa caaacatagg cccactaaca aaatgattgc taaataattg atttttttca
    49741 ttgtcttcct cgttatcttt tattacaaaa aactattttt atgatagcct tgctaaaagg
    49801 aagagtcaat aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt
    49861 gagtaacatc ggacaaatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa
    49921 caagcttgca gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa
    49981 gtgcaacagt gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag
    50041 tttttgcatt tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag
    50101 cgaactcatc aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag
    50161 ctccttagct gcttgaattg caagcctgat atgcggatac cctaatatcg cttcttcata
    50221 tgcaccattt acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg
    50281 atattttgta tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga
    50341 aaaaggctgt tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca
    50401 aatcgctcct ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt
    50461 tcgcccaatc tcagcaattc tttctcttaa ctcctgatca atagcatgat gccatgctat
    50521 ttgagccatt tcttcaaaaa aaggagttag cgtcttagca gacttaagaa ataaagcata
    50581 gtccatatcg gaatgtgcac ccgcatccag tgcatccact aatccaggtt ttggacttaa
    50641 tgttacttct gcgatcaatg cttcctctac caacgcagcg agttgctgag tcactgcttt
    50701 ttttcgttcg tcatttatca tgacactcac tccttcttcc tactattact tgctgaaacc
    50761 ttttttgata gctacccaaa tgatatgaag taaccagctt aaagcaaatg cacaacttgc
    50821 cgtcattaga taagctacaa gaatcatgac tcctgaaggt aactgcagcc aactatcttt
    50881 aaacaataat aacagtactt ggaaaacaaa tacattggct aaatacgtac gataagcata
    50941 aacagacaac catttgaaca atggcaattt tggcgaatcg tttacaatca ttttggaagc
    51001 gcctatgaag acagcaaaga tgatgactaa actgtataaa ctttggatcg tattcaagta
    51061 aggtgcattc gcaaagctca agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa
    51121 cgcgataact aaggcaacag gtaagaaaag ataacgtact ctgtccaaat attgatagac
    51181 tttttcgtga taaacaaatg cggctacccc ataaatcatg aaactagcaa caaaccgatc
    51241 taataagtac cagctgtcat gataaggccc agtcaatact tgtgtttgat agaacagata
    51301 ccatgcgaca tataaaatcg ttgaaccaat caatactggc catacatatt ttttattagc
    51361 aaatactttg taaccgagcc aaacaaaaaa cggcatgaac aattggatct gcagcatcat
    51421 aacggtatac cataaatgag gcgctccatc acctgtgaca tatttcagta aaaagcttgc
    51481 agtatccgta tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt
    51541 ccatagaaga tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga
    51601 caaatgctga cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt
    51661 gtatttaacg atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt
    51721 tgccaaagca aagctcaata tcgtttgcat cataacagcc gttacagctg atactttctg
    51781 gaagtcactt acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt
    51841 ctttattacc aaagttcttt ggttaattta ggtccttcga ctgttttcaa tagaattttt
    51901 ttctctggct ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg
    51961 gtatcgaatt gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc
    52021 ggtgaatttg cgtacagcag caaatcaaaa tcactggttt cttttactgc cggaatccca
    52081 gtcgataatt caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt
    52141 ctagcagcat gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat
    52201 tcttgttcga tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct
    52261 gttgaccatc tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga
    52321 tccgtcgacg cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc
    52381 aaggtgaacc cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt
    52441 ttttatgcat cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg
    52501 aatgttttgg ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg
    52561 acatcttgaa tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca
    52621 gcttggtcgg tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg
    52681 atatcgtaag cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt
    52741 tttgtcacac gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt
    52801 aaagcaatca agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt
    52861 cctgcaatca atccaataac tggatgtcca gcttgacgga gttttgcata agcagcagca
    52921 ctattagcca aagccacatg gataccgatc aattcttcgt tatagccgta tgcttggctt
    52981 ggtacatcga tcacaacgat gattggccgt ttcacttttt tgtctttatc ttcttcgtaa
    53041 acgtgatcca aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt
    53101 acacggtaca tcgggttttt aggatcaggt acgattgatg tcacgatggc ttcttctatg
    53161 aatttacttg atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca
    53221 gttagtgccg caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct
    53281 acttctggta agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca
    53341 atggccattg gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt
    53401 tcactgcgat gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct
    53461 tctacactat cagtcactaa ttcatcaatg atttttgatt ctacccgttg acgagatcct
    53521 aatgtattcc aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt
    53581 tcaataactt ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc
    53641 attaatgctg aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg
    53701 atgactggta catattgttt tagagcaatg attacatttc cgatttctga aatagataac
    53761 agtccgtagt ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag
    53821 atctcatttc cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca
    53881 ccggaaactt caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct
    53941 tgtttgccat tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt
    54001 ccttgtgctg gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca
    54061 ttgtcaagta gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc
    54121 tgaaagttcc tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa
    54181 gtcattgatc acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt
    54241 gttccatact tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt
    54301 ttctgttgga aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt
    54361 cttattgatc ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc
    54421 tttttgtaaa acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa
    54481 atggcgacaa aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt
    54541 tcatacaggc catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa
    54601 cgtttcgctt cactacgttt gatgcctaga tcttctggta atttcacgat gccggctgca
    54661 cgcaagctat ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt
    54721 gctgcaattg ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata agcaattcct
    54781 tcttctgtca cgatatgtgt agtatcttcg ctataaatca tcactggtgc attggctaag
    54841 cctgcttgtt tttgaacagc tagtgcatct aattcttcga caaagactgg ttttttattt
    54901 gaaccatatg tttcaaccat ttgaacaact aatttttggc ctttccctaa tggatcatca
    54961 ttgtcgcgaa gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc
    55021 atgttaggcg caccgccaaa accagataaa cgtccttttg ttacggtaga agagtttcct
    55081 tcgtaatcta attgcaaggt tgaaccgata aacatatcga tggcatattg tcctgcagct
    55141 tgtgataatg ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg
    55201 cgagcagcaa tatatttttc cataccaact tcgccaccaa aactatggat gctttcaacc
    55261 caacctgatt cgatggctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg
    55321 attttccctt ttaaccctag tgattcacca taagttggca gtaacaattc gatagctgct
    55381 gtattgaatc caataccgtg attcaaagat tgtacgccgt gttctgcgta gattcctttg
    55441 attgccatca ttgccattag aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat
    55501 aaagcttcta attgataagg tttatctgct ttcacgacaa cgtctaccca gtcaccaggg
    55561 atatctacac gtggtaaaga atcgacaact tcgttgactt gaacgatcac gataccatct
    55621 ttgaaagctg cagcttcgat gattgttggt gtttcttccg tattgaaacc tgtgtacaag
    55681 ttgccgtctt tatctgcttt atctgcagct actaaaacaa catttggaat caaatcgatg
    55741 aataagcgtc cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct
    55801 tcaatcattt gggcgacacg caagctttgt gcaccagcat aagagaaatc gatcttgctt
    55861 gcgattcctt tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa
    55921 tgcagatcat tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct
    55981 tgtttttgat tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt
    56041 acttctacga ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc
    56101 aatcgctgcg cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt
    56161 agtcatctcc atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg
    56221 atcgtacgga tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg
    56281 ccggggggaa attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg
    56341 ttccactgca cagggaaagc taggttttcc aacaaatctt tacgaatctc ttctgcgtcc
    56401 tgagtggctc ttccatccgt attttttata tacaaacatc tagcatcaga tagatggaca
    56461 gactgcatat acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc
    56521 acagggactt tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta
    56581 acggataaac cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac
    56641 agataaactg gatggtcagg atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc
    56701 gtcacaccta ctactacgcc cattgcataa ccgcttggat aagcttcttt cattaagact
    56761 gcgcggtgat agactagttc cgctgcattc tcaaatctca aagatcgaca agcaacagct
    56821 gcacaaaaag caccaatcga atgtcctgct gcaatatcag gctgaatacc gtttttttcc
    56881 atttcatcta aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct
    56941 tgatagtttt cttctttatc aactaaccga taccccgtca cattttctaa acgttccatg
    57001 tacatttcag ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag
    57061 aaacctgtac tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc
    57121 aatttctgct ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat
    57181 gggatggata attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa
    57241 aaatgttacc caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat
    57301 caaacatttg gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga
    57361 gacgaccgtt tatgcagaag aattttatcc atatgcagtc aagacattag acacattaga
    57421 agaaggaatc aaattattgt cttcggtcaa tgagaagatc gttacttccg tcatattgta
    57481 tttagaggta gcctctgttt ctattccgaa cttggtccga atttttatgg aaaagcatcc
    57541 tgatatccaa ttaacgatca tgcaacatca tttaccagat gatattaccg atcctgtact
    57601 gtacatcact tcggagatga agcctggcat gaccaatatt cctatcatga aagaacctgt
    57661 ttatgtcgcc attcctaaga aacatcctct tgctaaaaaa gaacatctgc aattagaaga
    57721 tatccaacgc acccccatct tgatgctgtc aaagagcaac gccttccgtc atacactgga
    57781 tgcagcactt gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag atgatcctgc
    57841 gacattacgt tctattgtca agcaaggatt agctgttagc tttttcccaa aagtttcttg
    57901 gtcttacgat aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg
    57961 aacaatttat ttatcttcta gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa
    58021 aacgatgcgg gaattttatt taggaaaata aaaaaaaaag agctgggaca aaattcttgt
    58081 cgcagctctt ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa ataagccgaa
    58141 atttcccaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctaaac
    58201 atttctgtca caacctcttc tctattgtcc tagacattca ttgcgt
//
LOCUS       DAZKCN010000007        55855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000007 DAZKCN010000000
VERSION     DAZKCN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.007"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..98
                     /locus_tag="ACG8K3_000346"
     tRNA            25..98
                     /locus_tag="ACG8K3_000346"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Arg,seq:tct)
     gene            257..616
                     /locus_tag="ACG8K3_000347"
     CDS             257..616
                     /locus_tag="ACG8K3_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP9144256.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            739..2370
                     /locus_tag="ACG8K3_000348"
     CDS             739..2370
                     /locus_tag="ACG8K3_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP9144257.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMLQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(2432..3073)
                     /locus_tag="ACG8K3_000349"
     CDS             complement(2432..3073)
                     /locus_tag="ACG8K3_000349"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9144258.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    3259..3335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3355..3663
                     /gene="rplU"
                     /locus_tag="ACG8K3_000350"
     CDS             3355..3663
                     /gene="rplU"
                     /locus_tag="ACG8K3_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9144259.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3690..4034
                     /locus_tag="ACG8K3_000351"
     CDS             3690..4034
                     /locus_tag="ACG8K3_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP9144260.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            4047..4340
                     /gene="rpmA"
                     /locus_tag="ACG8K3_000352"
     CDS             4047..4340
                     /gene="rpmA"
                     /locus_tag="ACG8K3_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9144261.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4774..7581
                     /locus_tag="ACG8K3_000353"
     CDS             4774..7581
                     /locus_tag="ACG8K3_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9144262.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7772..8263
                     /locus_tag="ACG8K3_000354"
     CDS             7772..8263
                     /locus_tag="ACG8K3_000354"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9144263.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            8366..9355
                     /locus_tag="ACG8K3_000355"
     CDS             8366..9355
                     /locus_tag="ACG8K3_000355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP9144264.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            9388..10191
                     /locus_tag="ACG8K3_000356"
     CDS             9388..10191
                     /locus_tag="ACG8K3_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9144265.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            10212..11126
                     /locus_tag="ACG8K3_000357"
     CDS             10212..11126
                     /locus_tag="ACG8K3_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9144266.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            11209..11583
                     /locus_tag="ACG8K3_000358"
     CDS             11209..11583
                     /locus_tag="ACG8K3_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP9144267.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11722..11967
                     /locus_tag="ACG8K3_000359"
     CDS             11722..11967
                     /locus_tag="ACG8K3_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9144268.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            12139..13203
                     /locus_tag="ACG8K3_000360"
     CDS             12139..13203
                     /locus_tag="ACG8K3_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP9144269.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            13449..13874
                     /locus_tag="ACG8K3_000361"
     CDS             13449..13874
                     /locus_tag="ACG8K3_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9144270.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13867..14316
                     /gene="nusB"
                     /locus_tag="ACG8K3_000362"
     CDS             13867..14316
                     /gene="nusB"
                     /locus_tag="ACG8K3_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9144271.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            14413..15267
                     /locus_tag="ACG8K3_000363"
     CDS             14413..15267
                     /locus_tag="ACG8K3_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP9144272.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            15268..16605
                     /gene="xseA"
                     /locus_tag="ACG8K3_000364"
     CDS             15268..16605
                     /gene="xseA"
                     /locus_tag="ACG8K3_000364"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9144273.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16610..16834
                     /locus_tag="ACG8K3_000365"
     CDS             16610..16834
                     /locus_tag="ACG8K3_000365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9144274.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16836..17726
                     /locus_tag="ACG8K3_000366"
     CDS             16836..17726
                     /locus_tag="ACG8K3_000366"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9144275.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17735..18553
                     /locus_tag="ACG8K3_000367"
     CDS             17735..18553
                     /locus_tag="ACG8K3_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP9144276.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18660..19109
                     /locus_tag="ACG8K3_000368"
     CDS             18660..19109
                     /locus_tag="ACG8K3_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9144277.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            19127..20809
                     /gene="recN"
                     /locus_tag="ACG8K3_000369"
     CDS             19127..20809
                     /gene="recN"
                     /locus_tag="ACG8K3_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9144278.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20864..20980)
                     /locus_tag="ACG8K3_000370"
     CDS             complement(20864..20980)
                     /locus_tag="ACG8K3_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP9144279.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21053..22009)
                     /locus_tag="ACG8K3_000371"
     CDS             complement(21053..22009)
                     /locus_tag="ACG8K3_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9144280.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            22180..22548
                     /locus_tag="ACG8K3_000372"
     CDS             22180..22548
                     /locus_tag="ACG8K3_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP9144281.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22718..23149
                     /gene="mraZ"
                     /locus_tag="ACG8K3_000373"
     CDS             22718..23149
                     /gene="mraZ"
                     /locus_tag="ACG8K3_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9144282.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            23170..24129
                     /gene="rsmH"
                     /locus_tag="ACG8K3_000374"
     CDS             23170..24129
                     /gene="rsmH"
                     /locus_tag="ACG8K3_000374"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9144283.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            24134..24535
                     /gene="ftsL"
                     /locus_tag="ACG8K3_000375"
     CDS             24134..24535
                     /gene="ftsL"
                     /locus_tag="ACG8K3_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9144284.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24536..26728
                     /locus_tag="ACG8K3_000376"
     CDS             24536..26728
                     /locus_tag="ACG8K3_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9144285.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSTYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26754..27716
                     /gene="mraY"
                     /locus_tag="ACG8K3_000377"
     CDS             26754..27716
                     /gene="mraY"
                     /locus_tag="ACG8K3_000377"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9144286.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27730..29091
                     /gene="murD"
                     /locus_tag="ACG8K3_000378"
     CDS             27730..29091
                     /gene="murD"
                     /locus_tag="ACG8K3_000378"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9144287.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            29117..30205
                     /gene="murG"
                     /locus_tag="ACG8K3_000379"
     CDS             29117..30205
                     /gene="murG"
                     /locus_tag="ACG8K3_000379"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9144288.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            30220..31440
                     /locus_tag="ACG8K3_000380"
     CDS             30220..31440
                     /locus_tag="ACG8K3_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP9144289.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31596..32921
                     /gene="ftsA"
                     /locus_tag="ACG8K3_000381"
     CDS             31596..32921
                     /gene="ftsA"
                     /locus_tag="ACG8K3_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9144290.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32944..34185
                     /gene="ftsZ"
                     /locus_tag="ACG8K3_000382"
     CDS             32944..34185
                     /gene="ftsZ"
                     /locus_tag="ACG8K3_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9144291.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            34202..34879
                     /locus_tag="ACG8K3_000383"
     CDS             34202..34879
                     /locus_tag="ACG8K3_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP9144292.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34893..35492
                     /locus_tag="ACG8K3_000384"
     CDS             34893..35492
                     /locus_tag="ACG8K3_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP9144293.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35573..35794
                     /locus_tag="ACG8K3_000385"
     CDS             35573..35794
                     /locus_tag="ACG8K3_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP9144294.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35843..36625
                     /locus_tag="ACG8K3_000386"
     CDS             35843..36625
                     /locus_tag="ACG8K3_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP9144295.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36646..37353
                     /locus_tag="ACG8K3_000387"
     CDS             36646..37353
                     /locus_tag="ACG8K3_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP9144296.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37615..40413
                     /gene="ileS"
                     /locus_tag="ACG8K3_000388"
     CDS             37615..40413
                     /gene="ileS"
                     /locus_tag="ACG8K3_000388"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9144297.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40462..41988)
                     /gene="zwf"
                     /locus_tag="ACG8K3_000389"
     CDS             complement(40462..41988)
                     /gene="zwf"
                     /locus_tag="ACG8K3_000389"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9144298.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(42003..42650)
                     /locus_tag="ACG8K3_000390"
     CDS             complement(42003..42650)
                     /locus_tag="ACG8K3_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP9144299.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42834..43163)
                     /locus_tag="ACG8K3_000391"
     CDS             complement(42834..43163)
                     /locus_tag="ACG8K3_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP9144300.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43340..44068
                     /gene="sfsA"
                     /locus_tag="ACG8K3_000392"
     CDS             43340..44068
                     /gene="sfsA"
                     /locus_tag="ACG8K3_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9144301.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            44084..45097
                     /locus_tag="ACG8K3_000393"
     CDS             44084..45097
                     /locus_tag="ACG8K3_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9144302.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            45097..46374
                     /locus_tag="ACG8K3_000394"
     CDS             45097..46374
                     /locus_tag="ACG8K3_000394"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9144303.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46437..49139
                     /locus_tag="ACG8K3_000395"
     CDS             46437..49139
                     /locus_tag="ACG8K3_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9144304.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49291..49608
                     /locus_tag="ACG8K3_000396"
     CDS             49291..49608
                     /locus_tag="ACG8K3_000396"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9144305.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49638..49958
                     /locus_tag="ACG8K3_000397"
     CDS             49638..49958
                     /locus_tag="ACG8K3_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9144306.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            50066..51526
                     /locus_tag="ACG8K3_000398"
     CDS             50066..51526
                     /locus_tag="ACG8K3_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9144307.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51594..51815
                     /locus_tag="ACG8K3_000399"
     CDS             51594..51815
                     /locus_tag="ACG8K3_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144308.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51846..52028
                     /locus_tag="ACG8K3_000400"
     CDS             51846..52028
                     /locus_tag="ACG8K3_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP9144309.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            52028..52441
                     /locus_tag="ACG8K3_000401"
     CDS             52028..52441
                     /locus_tag="ACG8K3_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP9144310.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52564..53745
                     /locus_tag="ACG8K3_000402"
     CDS             52564..53745
                     /locus_tag="ACG8K3_000402"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144311.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53816..53980)
                     /locus_tag="ACG8K3_000403"
     CDS             complement(53816..53980)
                     /locus_tag="ACG8K3_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144312.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            54134..54207
                     /locus_tag="ACG8K3_000404"
     tRNA            54134..54207
                     /locus_tag="ACG8K3_000404"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54168..54170,aa:Arg,seq:cct)
     gene            complement(54276..55415)
                     /locus_tag="ACG8K3_000405"
     CDS             complement(54276..55415)
                     /locus_tag="ACG8K3_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9144313.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(55525..>55855)
                     /locus_tag="ACG8K3_000406"
     CDS             complement(55525..>55855)
                     /locus_tag="ACG8K3_000406"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP9144314.1"
                     /translation="IENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSR
                     TAGEYFLYDENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGE
                     AGTTEYK"
BASE COUNT        18373 a         9452 c        11970 g        16060 t
ORIGIN      
        1 attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc
       61 taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga
      121 ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata
      181 aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag
      241 aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga
      301 atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt
      361 ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca
      421 aggagtgtac aatggactat taggagtggg actactttat ggagcttatc tttcttccgc
      481 atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag
      541 tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct
      601 ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa
      661 aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat
      721 gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca
      781 ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta
      841 gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg
      901 attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata
      961 tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg
     1021 caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc
     1081 cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt
     1141 attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt
     1201 acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc
     1261 tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg
     1321 aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc
     1381 catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg
     1441 aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt
     1501 gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat
     1561 aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct
     1621 ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct
     1681 ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt
     1741 gtttttgcgc cagtgatgtt acagattcca atgccagcac ttgcaggtgt tttgattgtg
     1801 acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg
     1861 tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt
     1921 gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc
     1981 acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg
     2041 aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc
     2101 aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta
     2161 ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa
     2221 agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac
     2281 ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta
     2341 catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa
     2401 actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta
     2461 cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat
     2521 gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg
     2581 ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt
     2641 tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct
     2701 tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc
     2761 gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat
     2821 acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag
     2881 aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct
     2941 ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag
     3001 cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt
     3061 ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat
     3121 gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt
     3181 tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta
     3241 ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata
     3301 tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac
     3361 gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt
     3421 gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc
     3481 ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt
     3541 gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca
     3601 caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct
     3661 taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg
     3721 caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg
     3781 atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat
     3841 tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg
     3901 aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc
     3961 ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa
     4021 ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc
     4081 acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg
     4141 ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta
     4201 caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag
     4261 ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc
     4321 cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca agacttttgt
     4381 cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag tttactttgt
     4441 ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat aactatcgtt
     4501 ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg taaacagtgt
     4561 tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt taaattgaac
     4621 atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag
     4681 aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag aaaaggagcg
     4741 ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat
     4801 ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga agtgttaggg
     4861 attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg aaaactgata
     4921 aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca cgtaaagcca
     4981 gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa aaatacactc
     5041 caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag catgaaaaat
     5101 tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa ttgtttgatt
     5161 acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca ttttgcaaaa
     5221 gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc
     5281 aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc ttttacaggc
     5341 gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct gtttttggat
     5401 gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat ggatcatgga
     5461 acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt
     5521 gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg tatcccaatc
     5581 aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa
     5641 atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac agaagatgta
     5701 gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa gtcaaatatc
     5761 cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat ctcgatcaca
     5821 atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag caaccgcagt
     5881 gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc aaatgatacg
     5941 atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga tatcatcgga
     6001 gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa tcatttcatt
     6061 tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt ttcttttaat
     6121 gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac caatcagatt
     6181 tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat ttatgctatg
     6241 agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg gcatacgaat
     6301 gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc gaaagaagtg
     6361 cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga ctattacttg
     6421 accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt
     6481 gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca gttgttagat
     6541 gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac agcattggac
     6601 cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact cttagtagat
     6661 atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat tccagtacgt
     6721 actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt ctcagtatta
     6781 gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata
     6841 tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc tgttttggct
     6901 atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga acgagcggta
     6961 aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga agtgaaagaa
     7021 gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat tactgatttg
     7081 aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat cgaagtcatt
     7141 ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc cctagatgaa
     7201 gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt tattaatggt
     7261 agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac gacgggaatc
     7321 ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg aatgatcgaa
     7381 cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa tacaacgaat
     7441 gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca gataaaagta
     7501 aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca gttagataaa
     7561 aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg tatcgttttt
     7621 gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt
     7681 tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat aggggtttct
     7741 gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac gtatagatga
     7801 tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat
     7861 cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac taaaagaagc
     7921 tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc aaatctatca
     7981 tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc aagatgttga
     8041 agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta tgagttttac
     8101 tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata ttgattcctt
     8161 tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg ctgacaggaa
     8221 aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga
     8281 aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga atttgttaca
     8341 acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc atggacaatt
     8401 tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa acgtcaaagc
     8461 cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg aagaagcggt
     8521 tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg gcggaacacc
     8581 gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg
     8641 tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg aatctgctca
     8701 tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga
     8761 attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta aaggagcact
     8821 tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac gtgttgactc
     8881 acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat
     8941 tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa tcgaacaagc
     9001 tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg aagtagcaaa
     9061 agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg aagacgtatt
     9121 gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa tggcgcattc
     9181 ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg ttgaagcgtt
     9241 tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg
     9301 cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat
     9361 aatattaaaa tttataggag gcttatcatg tctattattt caataatttt agtcgtatta
     9421 gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca tcaaccacta
     9481 gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat cgtacttggc
     9541 ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt agcaccagat
     9601 gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca aggtgttaaa
     9661 ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt gttcttaaca
     9721 atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc tgctgaagaa
     9781 ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca aggggttcgt
     9841 atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca atcattcctt
     9901 gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat ggtcgtagct
     9961 gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc attcttcgtt
    10021 attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg tgctttaggt
    10081 gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc aaataatgga
    10141 ggcggaagca actctggtga ccctttaggc gacattttga atgactatta atcttgaagg
    10201 aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg
    10261 cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa aacggtggct
    10321 gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg
    10381 cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct tctccaattt
    10441 taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag
    10501 cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt gacccagtgt
    10561 tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct atgggaggaa
    10621 atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg gcattcatgt
    10681 ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac ctttctggcg
    10741 gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta ttagctgccc
    10801 ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta aaattagata
    10861 aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc aagagtgcat
    10921 ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca
    10981 acttagacca attgattcct ggtttagctg cattaggatt aacattgtta tgtatgtggt
    11041 tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta gtcggtgttg
    11101 ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt tgtcccgagc
    11161 tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat ggttcaatca
    11221 atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct gacagattac
    11281 gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga tagacgcaaa
    11341 tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat gctaaaggga
    11401 aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac attttcttcc
    11461 aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga tagaatggta
    11521 caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag
    11581 taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt tgcagaagaa
    11641 aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct aaaattgatt
    11701 agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg agccgtgatc
    11761 ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt aacgaatatc
    11821 attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg gggaccaaaa
    11881 gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt agcagttgtc
    11941 tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa aaggagttgc
    12001 agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag ttttccggaa
    12061 atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa
    12121 gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa
    12181 gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt aacgaacttt
    12241 acaggtacga caggattagc tgttattact ttggaaaaag cattttttat tactgatttt
    12301 cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa aaatgtcgga
    12361 cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc attagctttt
    12421 gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat cgatgcggaa
    12481 ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga agaagaaatc
    12541 gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat cctgaaaatg
    12601 atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta tatgcgttct
    12661 cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg ttcggctatg
    12721 ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac attggacttt
    12781 ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat tggtgatcct
    12841 ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc agttattgat
    12901 gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga ttatatttca
    12961 aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg tttagagatc
    13021 cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc
    13081 actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga ggatgacttg
    13141 ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt gatcatctta
    13201 tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa
    13261 aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag ataataaagt
    13321 tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt tcttaccacg
    13381 tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc aaagggagga
    13441 ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat ttaggagaga
    13501 tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct aaagtagaag
    13561 gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta ggacgtgcag
    13621 ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta gatctttact
    13681 gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag gaacgggtca
    13741 gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc catgtagtag
    13801 ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa gacgaagagg
    13861 aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt gcaggcttta
    13921 tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa cgcaatcgaa
    13981 ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt ttatcttgat
    14041 acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat
    14101 ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat cttgcgtatc
    14161 gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca
    14221 atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa cggtgtttta
    14281 tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt ctatgaatca
    14341 tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca aaaaatatct
    14401 gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa aatgcaggca
    14461 gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg attagtcgtt
    14521 ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga acgtgtagca
    14581 aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc tgaagacgaa
    14641 ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat attggtccaa
    14701 ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg ccctgaaaaa
    14761 gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa accggacaag
    14821 attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat cgatccggcg
    14881 ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta
    14941 ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa tctggctgaa
    15001 ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt tgtcacaaaa
    15061 gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga tgctaacggt
    15121 aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg gatcactcct
    15181 gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac cgtaaaatct
    15241 gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt cactacatta
    15301 acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt atatttaaca
    15361 ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag cttaaaagat
    15421 gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt acgattccag
    15481 ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga agcgggaggt
    15541 tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt atatcaagaa
    15601 ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg
    15661 ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc ggttatccgc
    15721 gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt gttcccaaca
    15781 gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag agtagaagag
    15841 atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga ggatttatgg
    15901 ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc ggtgatttca
    15961 tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt tcgggctgcc
    16021 actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt gatgcgcatt
    16081 caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg caaacaagaa
    16141 cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt
    16201 cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca gcaaatttat
    16261 catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt ggctccgctt
    16321 tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt agaagagaaa
    16381 atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca atctcttgat
    16441 ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt aaatgatcaa
    16501 gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca ttatcaagat
    16561 ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata tggaaaatcc
    16621 aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag aacaaggaga
    16681 cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa gcaaacattg
    16741 ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag aatcaaatga
    16801 agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac ttttcagcta
    16861 ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat acaacggata
    16921 gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga atacgtccct
    16981 tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc gtctatcaag
    17041 tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat ttgcctgcga
    17101 tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc ggcgaagcat
    17161 tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc agtatggcac
    17221 acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt gcgggatcac
    17281 aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag ctttctttag
    17341 aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat gccttgctgg
    17401 ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa cgattagccg
    17461 aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt ggtacaacga
    17521 aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt
    17581 tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc gccaacaaga
    17641 tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg cgccagatga
    17701 tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg tagatgtatt
    17761 agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag tgatggctgg
    17821 tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag
    17881 tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg gattaaaatt
    17941 agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt tagatattgg
    18001 tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc
    18061 tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta gagtcgtcgt
    18121 aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag gaattccaga
    18181 agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc cattacatgc
    18241 tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg aagcaggcag
    18301 agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa aagttttaga
    18361 agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa gttattctcc
    18421 gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag tacccgaaag
    18481 cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac acgaacagtt
    18541 tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat tcttctttat
    18601 tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc gaagacgtaa
    18661 tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa
    18721 agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact caagctacga
    18781 tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat ggcggctacc
    18841 gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa aaactattaa
    18901 aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa acattgccgg
    18961 gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa ttattttcga
    19021 ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag gcagttttat
    19081 tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc tacaagaaat
    19141 cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg aaggaatgac
    19201 cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt taggtctgtt
    19261 agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat gtgtgcttga
    19321 agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg aattagggat
    19381 cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg gaaaaaatgt
    19441 ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg gcagttattt
    19501 agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat cacatcttgc
    19561 tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt atcagcaaga
    19621 atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc
    19681 ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg ctgctgattt
    19741 gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca attatcaaaa
    19801 aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac aaaacagtgt
    19861 agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag atgtggagta
    19921 tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg caatcggcga
    19981 tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag aagttacgca
    20041 acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg agtcaatatt
    20101 ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag aaggtcaatt
    20161 ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac aagccgcaga
    20221 tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc tgcatgaatt
    20281 gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag aagaaaatag
    20341 acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca atccaggaga
    20401 gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc
    20461 tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg atgaagtgga
    20521 tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc aaatatcgaa
    20581 atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg ccgactatca
    20641 atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt cagagttgaa
    20701 aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga ttacaaaact
    20761 cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag ccacctcgca
    20821 gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg acactcatta
    20881 cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg acaaattttg
    20941 ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac ctcgtcatta
    21001 attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt ttttataaaa
    21061 acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt aatagaacta
    21121 gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt ccatagatac
    21181 tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt ttcataatat
    21241 tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct actagtttta
    21301 attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca cgtaaatgat
    21361 ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc aatgcatcta
    21421 aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc atgatttgat
    21481 aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg cattgagatt
    21541 gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga
    21601 tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact ttctttaaaa
    21661 acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa ataatagaga
    21721 gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa ggatattgta
    21781 acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta cgggaatttt
    21841 ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct
    21901 gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg tcagtttcct
    21961 ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg attttcctcc
    22021 tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa tgtgtttgta
    22081 agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta aagtatatac
    22141 agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt cacaccactt
    22201 atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa
    22261 ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat tccttttttg
    22321 tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt accgtttttc
    22381 tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata ttattatgaa
    22441 gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct gttgacaatc
    22501 gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa aaaatagaaa
    22561 agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa atgtttatga
    22621 ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg gattgtggta
    22681 gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg aattccaaca
    22741 taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga
    22801 aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga tgagcgaatg
    22861 ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg cacgtacttt
    22921 tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag gacgaatcaa
    22981 tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag tcatcggtgt
    23041 atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg cagcagcaga
    23101 agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag aataggaaga
    23161 ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga aacggtagat
    23221 ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg tgccggacat
    23281 agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt tgaccaagat
    23341 caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca aggtatggtc
    23401 acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca tgtagataag
    23461 gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga agcagaacgt
    23521 ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc agctccttta
    23581 tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat tttcttccgc
    23641 tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt acgaaaaaca
    23701 aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat cccagctccc
    23761 gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg gattgcagta
    23821 aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt attgaaagtc
    23881 aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt caaaagtatg
    23941 tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat acctgacgaa
    24001 ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga ggaagagcta
    24061 acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat aagaatgaaa
    24121 gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg atttacctga
    24181 attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag aaatcattct
    24241 tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa ttgctgtggc
    24301 aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc gcacaaatat
    24361 cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata aaaagcaaga
    24421 aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga ttaaacaaat
    24481 cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga agtaaatgag
    24541 tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga tgaacaatcg
    24601 caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt tatttgccgt
    24661 ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag ctgaaaaaac
    24721 aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga tttatgatcg
    24781 taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga tactctcaaa
    24841 aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg ataaaatagc
    24901 agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata ttttagagga
    24961 cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa atatctccca
    25021 agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg gtctttattt
    25081 tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt ttattggata
    25141 cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg agtctgctta
    25201 caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata attttcaaaa
    25261 tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag atatctacac
    25321 gactttagac agcagactac aaagttattt agaaacactg atggatcaag taaatgaaga
    25381 atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag aaatcttggc
    25441 aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg gaaaagatgc
    25501 tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca tgaaagtttt
    25561 cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct tccaatctgg
    25621 aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa aaggtgtgct
    25681 gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac tggaacagcg
    25741 attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa gtactcattc
    25801 tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg accgagcaat
    25861 gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag ctttcacttc
    25921 tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg tggatcccca
    25981 aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt caaaagaaac
    26041 aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact acggtagtac
    26101 ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaccggaa cagcccaaat
    26161 cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt cgattgtgga
    26221 gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac accctaagac
    26281 atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg caatggattt
    26341 taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat ccgtagccga
    26401 ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg gactgcaacc
    26461 tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg gtgatcagtt
    26521 gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc ctgacgtaac
    26581 aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg aagttagttt
    26641 tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga
    26701 caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag agcatggaat
    26761 ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc gcaatgccgc
    26821 tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa
    26881 agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt ctgatcggct
    26941 ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct actcttttca
    27001 tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat tttataaaaa
    27061 tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga cagattctag
    27121 ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct
    27181 tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc tggcttgttg
    27241 gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct ggcttaggaa
    27301 gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat gatgtattaa
    27361 tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac agaaaacctg
    27421 ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta gcggcaattt
    27481 ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat gttatggaaa
    27541 cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga atttttaaaa
    27601 tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag attgatacag
    27661 ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt tggtaggaga
    27721 tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt cttaggactc
    27781 gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt
    27841 aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc tttagggata
    27901 aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc attgatggtc
    27961 aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga aatgggtatt
    28021 ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat tattgggatt
    28081 acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact gaatgcagga
    28141 gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag tggtgtagcg
    28201 caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca actcatggga
    28261 atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc acatattgat
    28321 tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa aaacatgaca
    28381 gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt agcgcaaaga
    28441 acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct
    28501 gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga acttggcgta
    28561 ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa attgtatggt
    28621 atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc acatcgtacg
    28681 caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc aaccaatatt
    28741 ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt attggctggc
    28801 ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg aatcaaagca
    28861 atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc
    28921 gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt agaaaacagt
    28981 acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca atacccaaat
    29041 tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata gaaaaagtag
    29101 aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg gacatattta
    29161 tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg aattcatgta
    29221 tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa tgcctttcca
    29281 taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca agacggtccg
    29341 attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc aaccagatat
    29401 cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag ccaaaatggg
    29461 tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata aatttttaag
    29521 ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa
    29581 caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta aaaaatcaga
    29641 tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt ttggcggtag
    29701 tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat atattccttc
    29761 ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc taaataaaat
    29821 aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg cagaggtcat
    29881 ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag aattcacagc
    29941 tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc atcagactaa
    30001 aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata atgaactaac
    30061 aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat tgcaaaagca
    30121 aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc tttatgactt
    30181 agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa aaaagaacca
    30241 aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca aaaggctaac
    30301 cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga agacataaca
    30361 ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa
    30421 gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt
    30481 ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa
    30541 gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct gatttttttg
    30601 atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg caataagaca
    30661 gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa tgtttgggga
    30721 cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa
    30781 acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac agaatataaa
    30841 gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa tggaacagta
    30901 ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga gttcaaagac
    30961 tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga attacaaaaa
    31021 gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat ccaattgaat
    31081 atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca aatgaaatat
    31141 tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga agttggtatc
    31201 ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc ggaagaaaca
    31261 actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa
    31321 aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa tgattcaagt
    31381 gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa tgaaaattga
    31441 taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta tggtagaatt
    31501 ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag ttgttcgctt
    31561 gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga atgtatgtag
    31621 gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa
    31681 tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc attatcgttg
    31741 atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa gaaaaagcag
    31801 gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt
    31861 gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa gatgtcagaa
    31921 atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat
    31981 tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt ggaatgatcg
    32041 gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc atccataaca
    32101 tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt actcctttag
    32161 cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc gtcattgata
    32221 tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag tttaccaatc
    32281 ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta aatacgtcat
    32341 ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa cggacatcac
    32401 cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac
    32461 gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg
    32521 cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca ggcggagctg
    32581 ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat gtaaaattgt
    32641 atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata agtatcgtgg
    32701 attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa
    32761 cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat gagagctatg
    32821 aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa
    32881 aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg ggaggaataa
    32941 gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa agtcatcgga
    33001 gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt
    33061 gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt
    33121 atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc agaagttgga
    33181 caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg
    33241 attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc aatcgttgca
    33301 ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc attcactttt
    33361 gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg
    33421 gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa aaagacaccg
    33481 atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca aggtatctct
    33541 gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa aacagtgatg
    33601 gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga tcgcgtagta
    33661 gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga tggagctgaa
    33721 caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc acaagatgct
    33781 tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg tacatctatc
    33841 aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat cgatgaatca
    33901 aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc acctgctcaa
    33961 aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga aaacagtttt
    34021 ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga ttccaatttt
    34081 gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa atctcatggc
    34141 gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag aggaagagca
    34201 catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt
    34261 tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg aatccgctca
    34321 agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag tagataaatt
    34381 attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt taattggtaa
    34441 tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc atgcgttgga
    34501 cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat cctgttttat
    34561 tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg agcttttacc
    34621 gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga tgactatggc
    34681 gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga aagaattgcg
    34741 tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa gtatggggat
    34801 gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg taggaacagc
    34861 gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga ggagcaattg
    34921 ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat cctgaaacaa
    34981 caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca actcattctg
    35041 tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg
    35101 aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca cctacaaaaa
    35161 ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca aatcttgctg
    35221 gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt gctaaacgaa
    35281 tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa
    35341 gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc aaacgtgttg
    35401 gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca accgctcaat
    35461 cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt atactattgc
    35521 actattactt catttacttc aacgggcagc ctatttctat agcattatct tgatggtata
    35581 tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat tattagctaa
    35641 gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg gacccttgga
    35701 tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt tagcacagat
    35761 cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc tttattctca
    35821 gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc gggcagatga
    35881 acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac aatatgcacc
    35941 ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac tgattcgaca
    36001 aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa gaaaaagatg
    36061 tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag cattgtatga
    36121 aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg gaactttagt
    36181 gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg atcgctggca
    36241 agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa agattggaaa
    36301 ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga aagatagctg
    36361 gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct ctgagattta
    36421 taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg
    36481 gacagaaaat acaagacctg attttgtact tgaactatta gatattattt cagtcagagg
    36541 ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa agtacagggt
    36601 actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc tttaactcca
    36661 ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca agacgaagta
    36721 gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa
    36781 ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga attaaaagat
    36841 gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa aacaagtgca
    36901 agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga gttagttgcg
    36961 aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc aagaaaaatc
    37021 ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga tcttaagaaa
    37081 aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt agaacaagtg
    37141 aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac tgacagccat
    37201 gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac tgatgtaaac
    37261 tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc tattgatatc
    37321 tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg ttattcttaa
    37381 agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat cctctacttg
    37441 agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat tggtcacaga
    37501 gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt tcgtcccttg
    37561 cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa
    37621 atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac
    37681 cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag
    37741 ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc caatggtaat
    37801 atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg ttcaaaatca
    37861 atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt gccgattgaa
    37921 caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa
    37981 aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt caaacgccta
    38041 ggagttgcag gagattggga acatccttat gttacattag atccttctta tgaagctgca
    38101 caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg attgaaacca
    38161 atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga atataaagat
    38221 gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga tattcttgat
    38281 gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc aaaccaaggt
    38341 attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg taagtttgtc
    38401 gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt
    38461 ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc attctatgaa
    38521 cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac tggattagtc
    38581 catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata taaactatct
    38641 gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg atttgaaggt
    38701 atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa aggagctttg
    38761 ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa aaagcctgtg
    38821 atctatcggg caactccaca atggtttgca tctatcgata aattccgtca gaatattttg
    38881 gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt gtataatatg
    38941 atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt accacttccg
    39001 attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga acatgtggca
    39061 aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa agaacttctt
    39121 cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga aaaagatatc
    39181 atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca gcgaccagaa
    39241 ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg ctggttcaac
    39301 tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc agttatttct
    39361 caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg aaacatcatt
    39421 gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg ggtatctagt
    39481 gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt ggcagaagta
    39541 tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga tttcgatcct
    39601 aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat gaccgttcga
    39661 ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa cttcatgcat
    39721 atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta cctggatttt
    39781 gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg catgcaaact
    39841 gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat tccgcataca
    39901 gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt aagcgagttt
    39961 cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac tgctttcatg
    40021 gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa attgattggt
    40081 aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga aatgttaaaa
    40141 gctttggatg cagatattgc acaattattg atcgtttcac cagactactt tgaagttatg
    40201 gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc tattacagtg
    40261 gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta
    40321 gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct
    40381 gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt gacatgttct
    40441 ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat accagttatc
    40501 tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc
    40561 cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat
    40621 gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt tcgttccatc
    40681 accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt caaccatttc
    40741 tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc ctacttcttt
    40801 cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta gaacattagg
    40861 tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat taacaggaac
    40921 ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac
    40981 atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg cgacgaatgt
    41041 ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga attgtacacc
    41101 atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt cttcttcgtt
    41161 ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt tttcggaaaa
    41221 tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat tttgcaccat
    41281 gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac cgaggttttc
    41341 gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct caaaaatatt
    41401 attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat aatgatcaat
    41461 cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt caaaagcaga
    41521 ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat tttttgtcat
    41581 aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg ccatagccaa
    41641 ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag acaattcttt
    41701 taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa aatggcttgc
    41761 aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt tgacaacttc
    41821 acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa aatgctcgcc
    41881 taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct gggctaggtc
    41941 acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt ggatacactc
    42001 ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg gattttcttt
    42061 ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat ctcatattct
    42121 tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc atcaagcact
    42181 cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg gcgttcttca
    42241 tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc aggatgattc
    42301 aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa tacttcagca
    42361 tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac ttcccataag
    42421 cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct
    42481 tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat ttcactgaca
    42541 gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt cgttgtatct
    42601 ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat attcgatcat
    42661 cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta attgactatt
    42721 tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg aaagatgata
    42781 gagaagactc attctttcgt ttataaagga tatatatata cattgggagg gatctatttt
    42841 tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg ttcgggataa
    42901 gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc atctatttct
    42961 accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt
    43021 aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt tgagagtcct
    43081 tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag aacggtaatg
    43141 agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta tgagaaaata
    43201 atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc taaaatttct
    43261 ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg atacatagta
    43321 ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt ttattgaccg
    43381 cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta ttgcgcatgt
    43441 caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg ctattgtatt
    43501 tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa agaagggtga
    43561 ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta ttttagacgg
    43621 cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg aagtaactta
    43681 tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag catttgttga
    43741 agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg cgcccacact
    43801 cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt atgccgctta
    43861 tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg aagaaatgca
    43921 accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta
    43981 taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat ttgatgtaga
    44041 tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca acttaggtat
    44101 tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca caaaaaaata
    44161 tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg gttctcatta
    44221 tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta ctcatatgga
    44281 caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc aagcaatttt
    44341 ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag aaatggatga
    44401 aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa gaaatatcca
    44461 tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga taatcggtgc
    44521 aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg gagaagaacc
    44581 gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg tttaccttgt
    44641 atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc caagaaagat
    44701 ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt ttgatgtatc
    44761 gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt ttgactcagg
    44821 aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg atcgcgcgca
    44881 taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag atgaagcaga
    44941 ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt tgtatgaaga
    45001 gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa aaaatggcgg
    45061 catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt acaagtattt
    45121 cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc tcttattcaa
    45181 cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc tcctacaggt
    45241 tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc aggtgaagca
    45301 agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc agatgttgca
    45361 cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg agccaacgtc
    45421 aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac accaggaaga
    45481 atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac aatggtattt
    45541 gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca aattcttcat
    45601 caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg ttcccttgaa
    45661 agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc agaggacgat
    45721 tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa agatgactac
    45781 ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa tcaactgact
    45841 gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg aagtctggcc
    45901 tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc
    45961 agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg tcttccttat
    46021 gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc gggaagaact
    46081 ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa tctaagagat
    46141 ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt atacggaggt
    46201 aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat
    46261 gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa aataagaaaa
    46321 aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa ataagcgcct
    46381 ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg ggttatgtga
    46441 aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa
    46501 gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat ttaagtgaac
    46561 tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa
    46621 cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa
    46681 cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa gctgcgattc
    46741 tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca gggaagtcga
    46801 tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct gcagatgcgc
    46861 catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc tttggacaca
    46921 tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc ggtctaatag
    46981 ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg ccggaagggc
    47041 aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga gaaagtgcac
    47101 aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca gaaagtttct
    47161 tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt gcttcacgtt
    47221 cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg aatcgtttga
    47281 atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat gatgcggaag
    47341 gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct tttttacatt
    47401 accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc cttcaagtac
    47461 aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc atggacagca
    47521 aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca caagatccag
    47581 aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt tccgaaaccg
    47641 gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct tatttccaga
    47701 catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca gatgaaatct
    47761 ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt atttataatg
    47821 aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt gtgagagaag
    47881 gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag agggaattcc
    47941 aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag attccttttg
    48001 atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt gagcctgtta
    48061 aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct tcaagcatgt
    48121 tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat atgccactgg
    48181 ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac tatcaagagc
    48241 atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc ttcagtgatt
    48301 tgatttatga agagactggc attcggacga aagctatcac gatgacaagt accatgattg
    48361 tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt tatcaaaata
    48421 tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag tctgaaactt
    48481 tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta gctgccaaat
    48541 tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc cagatgcagt
    48601 ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa tatgagatca
    48661 aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt tcagcctatg
    48721 acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat gagatcccga
    48781 cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct attgatgaac
    48841 tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg attatcgtgg
    48901 aacctggagt agatactgga atcgttatcc atttagcctt catgttagac ggattgatca
    48961 aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg
    49021 aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag gtaaaaattc
    49081 cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata aaaaaatgaa
    49141 cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa aaagagctaa
    49201 aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg cgttatataa
    49261 aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt
    49321 tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa
    49381 gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa tttagaatct
    49441 aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc tcaatttgaa
    49501 caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga ctatggcatg
    49561 atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat caagtatgca
    49621 gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg gggttaatca
    49681 tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg
    49741 attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata caagcgcatc
    49801 atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa gtgacattat
    49861 tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat ttagcaaaag
    49921 aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt ttttgaacgg
    49981 aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc tctttatata
    50041 aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg agaaatacat
    50101 cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc gtgatgcttt
    50161 tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca atgcaattat
    50221 tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag cagacaaagg
    50281 aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga acggtacact
    50341 agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc gagcttatgg
    50401 agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag gtatttcctt
    50461 tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg cagatgcaat
    50521 caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg gattatgggg
    50581 ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga tcatgggagc
    50641 tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca aaatgcctga
    50701 ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt
    50761 atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc aactgatcat
    50821 tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag ggttgggagc
    50881 agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc atggatcaaa
    50941 tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca atattgatat
    51001 tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg caatcgaaga
    51061 tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt ttggcggatc
    51121 aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg cagattatct
    51181 aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt
    51241 tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg ctccagtagt
    51301 agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg tatcgcagca
    51361 agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag cggattggcg
    51421 ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg caccatttgt
    51481 catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg aacgtgagga
    51541 gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact gaaatgatta
    51601 caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga tacaatgaag
    51661 aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg
    51721 cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca cagccttggg
    51781 gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa gataggatgg
    51841 ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc gtgttgtata
    51901 gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt ctttttctaa
    51961 caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa
    52021 ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt acgataaagt
    52081 tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca
    52141 gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat
    52201 cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga agaatgaatt
    52261 cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc attacacaga
    52321 aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag gaacaatctt
    52381 gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag caacgtatta
    52441 aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag aaggaatttt
    52501 tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg aaggggcagc
    52561 aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat gtttgattta
    52621 gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt tcgtacactt
    52681 gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac aagtcaagta
    52741 atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc cggcattggc
    52801 tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat catggaaaag
    52861 atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc attctcctat
    52921 cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta tcaactaaac
    52981 agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa
    53041 gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca acgagctggt
    53101 ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt
    53161 gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca gcttgcaacg
    53221 tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg cagccttgtt
    53281 cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg cggctacggc
    53341 ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat cacaccttat
    53401 atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt taaaaagaga
    53461 caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa tgaaggaatc
    53521 gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca aacaaaactc
    53581 ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta tcgtaaagtg
    53641 gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga aacgttaagc
    53701 tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa aacgggtgat
    53761 gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga aaatgttaaa
    53821 gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta gggttttctt
    53881 ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta
    53941 agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat
    54001 tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac
    54061 ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg
    54121 atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg
    54181 tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt gtggtacagg
    54241 ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt atataaacta
    54301 tcgaatacag cattgatttt ttctttatcc tgatcttcaa gttctcgaac gatatgaaga
    54361 taagtagaca ttgtagtttc taaactgcta tgtcctaacc gttttgatac gctaagtata
    54421 tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg caaagtaaat
    54481 tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac ggcatttgat
    54541 gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt aacttcaaaa
    54601 ttctcgaata attctttttg attgatttta aactttttta agagatcaat cgtatgttta
    54661 tcaattgaaa ttttccgatt tgaagtctcg ttttttgttt ttccccattc atttaattta
    54721 taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc ttcccatgtt
    54781 aatcctagaa gttcagcaaa tcgcattcca gtagcaccag caactaacac gagcatagga
    54841 gaagagtact gtgcagaaag gttttcttct acgagtttca ttaaagattt aaattgatca
    54901 taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat tactgctttt
    54961 ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga tgctctaata
    55021 tggttattaa acttaataac ggtggatttt acatggtctt tcgcatactt atttaaaaag
    55081 cgttgatatc caactctatc taaatcagaa atcaatactg ctggcatata tttctttata
    55141 ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc ttctttgtag
    55201 agttgcatcc aatcttcaaa gtaatctgat aataatagat ttttgcgatc ccctttaaga
    55261 cctttattca ggttatattc taattcgttg gcggcatctt ttgcttcgcc ttttgtcttg
    55321 aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga aatttcgtat
    55381 tgccaactat ttccacgttt gactaatctt gccataattg aatctactct ctttctctga
    55441 tacaataggc actgtaaaga agcctattgt gtaggtttgt ttttaacgcg tccacaaact
    55501 ttggcgaggg agggggcgtg ttttttattt atactctgtt gttcctgctt cgccaatcgg
    55561 ctcagctgta aaatcatcag taaattgttt agacgcatta agtttaaatg tacttaaatt
    55621 tggtttattt ttagaaagta aatagaaaac cccagatgtt gtcgtttcag gttgaactaa
    55681 agaattagaa ttttcgtcat ataagaaata ttctccagct gttctactat attccatacc
    55741 gtctccatct attaaagatg cctgatacga agacaaagag aaaggtgatg ttgctttatt
    55801 ttttaacttg taagaaatag tagtacgata gtatttttct ggaaaatttt ctatt
//
LOCUS       DAZKCN010000008        54008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000008 DAZKCN010000000
VERSION     DAZKCN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.008"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1404)
                     /locus_tag="ACG8K3_000407"
     CDS             complement(40..1404)
                     /locus_tag="ACG8K3_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9144315.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1401..2960)
                     /locus_tag="ACG8K3_000408"
     CDS             complement(1401..2960)
                     /locus_tag="ACG8K3_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144316.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3016..5496)
                     /locus_tag="ACG8K3_000409"
     CDS             complement(3016..5496)
                     /locus_tag="ACG8K3_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144317.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5480..5815)
                     /locus_tag="ACG8K3_000410"
     CDS             complement(5480..5815)
                     /locus_tag="ACG8K3_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144318.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5829..6863)
                     /locus_tag="ACG8K3_000411"
     CDS             complement(5829..6863)
                     /locus_tag="ACG8K3_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9144319.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6953..7633)
                     /locus_tag="ACG8K3_000412"
     CDS             complement(6953..7633)
                     /locus_tag="ACG8K3_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9144320.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7917..9020)
                     /locus_tag="ACG8K3_000413"
     CDS             complement(7917..9020)
                     /locus_tag="ACG8K3_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP9144321.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9649..10119)
                     /locus_tag="ACG8K3_000414"
     CDS             complement(9649..10119)
                     /locus_tag="ACG8K3_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP9144322.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10449..12089
                     /locus_tag="ACG8K3_000415"
     CDS             10449..12089
                     /locus_tag="ACG8K3_000415"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP9144323.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12243..13565
                     /locus_tag="ACG8K3_000416"
     CDS             12243..13565
                     /locus_tag="ACG8K3_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP9144324.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13633..14067)
                     /locus_tag="ACG8K3_000417"
     CDS             complement(13633..14067)
                     /locus_tag="ACG8K3_000417"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144325.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14132..14968)
                     /locus_tag="ACG8K3_000418"
     CDS             complement(14132..14968)
                     /locus_tag="ACG8K3_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9144326.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15710..16336)
                     /locus_tag="ACG8K3_000419"
     CDS             complement(15710..16336)
                     /locus_tag="ACG8K3_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144327.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16631..16966)
                     /locus_tag="ACG8K3_000420"
     CDS             complement(16631..16966)
                     /locus_tag="ACG8K3_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9144328.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16953..17237)
                     /locus_tag="ACG8K3_000421"
     CDS             complement(16953..17237)
                     /locus_tag="ACG8K3_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9144329.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(17293..18816)
                     /locus_tag="ACG8K3_000422"
     CDS             complement(17293..18816)
                     /locus_tag="ACG8K3_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9144330.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18809..19984)
                     /locus_tag="ACG8K3_000423"
     CDS             complement(18809..19984)
                     /locus_tag="ACG8K3_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9144331.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(19988..20173)
                     /locus_tag="ACG8K3_000424"
     CDS             complement(19988..20173)
                     /locus_tag="ACG8K3_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144332.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(20139..21833)
                     /locus_tag="ACG8K3_000425"
     CDS             complement(20139..21833)
                     /locus_tag="ACG8K3_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9144333.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21830..22303)
                     /locus_tag="ACG8K3_000426"
     CDS             complement(21830..22303)
                     /locus_tag="ACG8K3_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9144334.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(22372..22527)
                     /locus_tag="ACG8K3_000427"
     CDS             complement(22372..22527)
                     /locus_tag="ACG8K3_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFP9144335.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22661..23041)
                     /locus_tag="ACG8K3_000428"
     CDS             complement(22661..23041)
                     /locus_tag="ACG8K3_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9144336.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(23045..23260)
                     /locus_tag="ACG8K3_000429"
     CDS             complement(23045..23260)
                     /locus_tag="ACG8K3_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144337.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(23263..23670)
                     /locus_tag="ACG8K3_000430"
     CDS             complement(23263..23670)
                     /locus_tag="ACG8K3_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFP9144338.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23936..25411)
                     /locus_tag="ACG8K3_000431"
     CDS             complement(23936..25411)
                     /locus_tag="ACG8K3_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFP9144339.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(25401..26249)
                     /locus_tag="ACG8K3_000432"
     CDS             complement(25401..26249)
                     /locus_tag="ACG8K3_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFP9144340.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(26286..26648)
                     /locus_tag="ACG8K3_000433"
     CDS             complement(26286..26648)
                     /locus_tag="ACG8K3_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144341.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26649..26807)
                     /locus_tag="ACG8K3_000434"
     CDS             complement(26649..26807)
                     /locus_tag="ACG8K3_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144342.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(26948..27572)
                     /locus_tag="ACG8K3_000435"
                     /pseudo
     CDS             complement(26948..27572)
                     /locus_tag="ACG8K3_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27569..27880)
                     /locus_tag="ACG8K3_000436"
     CDS             complement(27569..27880)
                     /locus_tag="ACG8K3_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144343.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27924..28217)
                     /locus_tag="ACG8K3_000437"
     CDS             complement(27924..28217)
                     /locus_tag="ACG8K3_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144344.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            28411..29058
                     /locus_tag="ACG8K3_000438"
     CDS             28411..29058
                     /locus_tag="ACG8K3_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9144345.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            29119..30264
                     /locus_tag="ACG8K3_000439"
     CDS             29119..30264
                     /locus_tag="ACG8K3_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9144346.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(30345..30650)
                     /locus_tag="ACG8K3_000440"
     CDS             complement(30345..30650)
                     /locus_tag="ACG8K3_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP9144347.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30675..31106)
                     /gene="ruvX"
                     /locus_tag="ACG8K3_000441"
     CDS             complement(30675..31106)
                     /gene="ruvX"
                     /locus_tag="ACG8K3_000441"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9144348.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(31103..31369)
                     /locus_tag="ACG8K3_000442"
     CDS             complement(31103..31369)
                     /locus_tag="ACG8K3_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP9144349.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31624..32349)
                     /locus_tag="ACG8K3_000443"
     CDS             complement(31624..32349)
                     /locus_tag="ACG8K3_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP9144350.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32710..32780)
                     /locus_tag="ACG8K3_000444"
     tRNA            complement(32710..32780)
                     /locus_tag="ACG8K3_000444"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32746..32748),aa:Gly,seq:ccc)
     gene            32897..33649
                     /locus_tag="ACG8K3_000445"
     CDS             32897..33649
                     /locus_tag="ACG8K3_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9144351.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33665..34144
                     /locus_tag="ACG8K3_000446"
     CDS             33665..34144
                     /locus_tag="ACG8K3_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP9144352.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(34395..35975)
                     /locus_tag="ACG8K3_000447"
     CDS             complement(34395..35975)
                     /locus_tag="ACG8K3_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP9144353.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35968..36711)
                     /locus_tag="ACG8K3_000448"
     CDS             complement(35968..36711)
                     /locus_tag="ACG8K3_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144354.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36887..37663)
                     /locus_tag="ACG8K3_000449"
     CDS             complement(36887..37663)
                     /locus_tag="ACG8K3_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9144355.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37911..38747)
                     /locus_tag="ACG8K3_000450"
     CDS             complement(37911..38747)
                     /locus_tag="ACG8K3_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP9144356.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38758..39687)
                     /locus_tag="ACG8K3_000451"
     CDS             complement(38758..39687)
                     /locus_tag="ACG8K3_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9144357.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39879..40232
                     /locus_tag="ACG8K3_000452"
     CDS             39879..40232
                     /locus_tag="ACG8K3_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9144358.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(40306..40962)
                     /locus_tag="ACG8K3_000453"
     CDS             complement(40306..40962)
                     /locus_tag="ACG8K3_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9144359.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            41188..43122
                     /locus_tag="ACG8K3_000454"
     CDS             41188..43122
                     /locus_tag="ACG8K3_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9144360.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(43208..44431)
                     /locus_tag="ACG8K3_000455"
     CDS             complement(43208..44431)
                     /locus_tag="ACG8K3_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9144361.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(44425..46296)
                     /locus_tag="ACG8K3_000456"
     CDS             complement(44425..46296)
                     /locus_tag="ACG8K3_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP9144362.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(46317..47435)
                     /locus_tag="ACG8K3_000457"
     CDS             complement(46317..47435)
                     /locus_tag="ACG8K3_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9144363.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47518..48351)
                     /locus_tag="ACG8K3_000458"
     CDS             complement(47518..48351)
                     /locus_tag="ACG8K3_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144364.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(48344..49261)
                     /locus_tag="ACG8K3_000459"
     CDS             complement(48344..49261)
                     /locus_tag="ACG8K3_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144365.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49488..50612)
                     /locus_tag="ACG8K3_000460"
     CDS             complement(49488..50612)
                     /locus_tag="ACG8K3_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9144366.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            50795..51703
                     /locus_tag="ACG8K3_000461"
     CDS             50795..51703
                     /locus_tag="ACG8K3_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9144367.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            51844..53457
                     /locus_tag="ACG8K3_000462"
     CDS             51844..53457
                     /locus_tag="ACG8K3_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9144368.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        16184 a        10811 c         9112 g        17901 t
ORIGIN      
        1 tttattatat ccccacacaa taaaagaccg tgatatttct cacagtcttt tatctactct
       61 attgctattt gctctcttag ctagtacttt aaaagctaac tcattgaggt agtgaaaacg
      121 agaggtgtgt tcagacagaa atccaaaatc ctcaactgct agctgtggaa tcgtagtttt
      181 gtgtttcacg atatccgcaa gcaggttcaa atctgataat aagaattctt ggcttatcag
      241 ctgtccgcca attatttcta atgtatccgg atggtagatc agctttgcat ttactgtttt
      301 gttttcttca tcgtattgtg gaaatagatc aaattctcct gaacacgaac cggtattttg
      361 ttccaagaat ggcgcttcat cttctgtcag ccctacagtt cctaaaaagt agtctataat
      421 gactgctgct gttatctttt gtctgaggtc gtaagcggtt ggctgaccac tcaaagttag
      481 cgctactgct cttgccatat gtacagctcg agtaactaaa ggtaaaaaga gatgccgttt
      541 atttttgtca aaactgactg ggacgatatc tccaatcgca tagatgtccg gatcacttgt
      601 ctgcagatat tcattgacta tcacagtctc atcctcatac aaagccactt tttcttttaa
      661 caatgtcgta ttaggacgag tttgggcaga aaaaatgacc atatctgcct ttattgcttg
      721 agatagaagt tgtacggata ctactttctc ttctgatttt ttccaatcga tcaagaattc
      781 atttggataa aaattgattt gcttagtttg cagctgattt tgaaaactgt tgatcatatc
      841 cttatcgaaa tatcttgata aaacattcgg catataatca actagatgaa taatgtatcc
      901 tttatttctc aaagcatctg ctaattccaa tccaatataa ccagcaccga agatcactac
      961 ttcttttgct tcttttaaag cggcaagtac ttgcttagat tgcactagat ttttataggt
     1021 gtagacattt tcagcttcaa tttgtttata caaggtgaca ggagaggaac ctgttgctac
     1081 gattaattta tcataggtta tggtctcgtt ttttccttct ttggatgcga ggatgagctg
     1141 atgtttgtct ggatcaagtt cagttactct ccattcggtg atcagttttg ccccactttc
     1201 ttgaagagtc cgcatgttat gggcaacttc agctaaacta cgtatctttc cttttaaata
     1261 aaggttgatt ccatttgaaa catagcctag tccatcttta tgatcttcat cgatcagtaa
     1321 aacttctgct ttcggttgaa gttttcttag atttagggca gctgtgatgc ctgcatggga
     1381 agcaccaatg atcacgattt tcatgtttta ttcatcctta aattcaattt ttctaaataa
     1441 cattctttta aaaattggtt caaatagggg aaatcataat tatattcgtt gctagtgaat
     1501 acaaaaactt tagcggactt ctgtgacggg tactctttca attggctagt caaaatgata
     1561 tcatactgct tttctttctc gtattcgact aaatcatatt tgatcgtatt cagcaattgt
     1621 tcctggatga tttctttcac gatatccgcc attagccttt cataaggaag atcataagca
     1681 atttgcagac gtaattctaa ctgcttgatc agcgtccgca aaatagatgc atagcgagca
     1741 aataattcaa agcgggcatt ttcctcgtct acttcagtaa aatcaacaat agcaaatgtt
     1801 tgtctgacca atctttcaca taaaaccagg aattgtttat cttctacttc agttaaccgc
     1861 tgttttaatc cttcttggcc aaaaatattc atccatcctc taaaataggc aaaatgagcg
     1921 tgattttgca tcactgtcaa cgcaatcttt tgcgaaatct cttctggaaa aacataaaaa
     1981 gagaaatatt cttctatcga ttgtttaaac gttcgattca attgagctac tacgtcgtct
     2041 ttgtattttt cgcttatcaa aaactcctgg acatgcgagt tatgtgtatc taatgtaaag
     2101 ttactgatca tgaaaagata aaaaaccaac gtttcatttt gattccaagg aaaggcatat
     2161 tgaaagaagt aacgggtcaa aaatgtatgg aggtccaata aaaattgtgt cgtttgaaac
     2221 aatccaacag gaagattgct ataatctggt ttggattttt tcgtacagcg cattcgcttc
     2281 ttcataatgg aaagccatat ttcaaatttc aatcgtcctt cttctgtaat agtaaaacta
     2341 aactcttttt gcagcagatc taaaaaagtg tttcctgata aatcactgta ttcttttttg
     2401 ttttcaagat aaggacgtcc aaaccaaaat aattggaaat aaaaataaca aatttgactt
     2461 tctggaccgg tcagccgccc gctttttatt ttcagatgaa actcatttaa agaatcattt
     2521 aattccttca ctctgcgaga caaagtagcc gaactgatat taagttccat gacaagtttc
     2581 tgccgatcaa atgagccatt ttccaacaga taaagaagta tctgatattt tgtagaatag
     2641 cgcaagtagc aatgataata aacatcgact gctgctatat ttccatgtct gattaataaa
     2701 atattccctt cttggataac aatttccaat tgtttgatat tttccaagtt agcttgtaaa
     2761 taaaacagat cttctttcaa ggtagcccga tcaactgata agcgggtaag tacgttatta
     2821 acggaaatct ctcctttttc ctctaataaa agttggaaaa gtttgatttg ccgttgttca
     2881 tttttttcaa gcaaactgaa aagttccaag tccactcctc caagcatttc tgattttaac
     2941 agcagaagtt tccttctcat ccttgaaaaa gaggatctgt gacagttatc acagatccat
     3001 acaaattcta atcacttagc tggttggccc agctgtcagt tcccatgtga ccatgccttg
     3061 atacgttgcg ccaggttgag cattcgtact gctatttacc tctaagagta aaccatcggt
     3121 atcgggaaag tcaacgagaa cctctccttt agctgttcca tcactctcat aaatcaacgt
     3181 gttttgacct tgctgaatca attgttttga tccagaatga ttgaagtata attttgttcc
     3241 gcttaattct tgaccttgat cattcttaaa taaagaagtc aatgtccctg tcagttgcca
     3301 aggagacgtg cttggaagcc gagtatcccg cacatcaaaa gaaaaatcgc cttttgcttc
     3361 ataataactt gtcttcaacg gtttggggag atcgttacca aatgtaaaat catctggtac
     3421 attaactaat gttaattctt gtttggggac aagaattgtt ttagcagatg tttggacaac
     3481 tgggttagga agcttagcac catctgctaa tgtaatattg ctggaaagta cagctgcctt
     3541 tgtcagtatg ctgacggatt gtacagaagt atctacagta tttaattgat agttgtaagt
     3601 aagatgaatg gtatctccta ttttgctcaa tagatctgct gggacagaaa ctccttgttg
     3661 aatgccggta tctgcgatag ttgtaccatt taattttaag gaaccattga tataagtagt
     3721 attttcagga agtgagatcg tgattttttt gtcggttatc gcatccccag attttgttaa
     3781 cgtgaatgtc acattttctt gaatcgtatc tcctgataga gcagacaggg gatctcctgt
     3841 ctcaggttgt ctgctaaggt tttttagctg actatctgca ctcacagatg gaaaattagc
     3901 cactgttatt tgaggaagag tggtgattcc agcttgttca gactgccatt tgtacgcaaa
     3961 agacggatac atgttagtaa gtacatcgtc caccaagcga taatcaaaat aatattgccc
     4021 tgaagccaat gcaggtagtg tagaagtagc tgtatttaca cccccgttat ttgattggat
     4081 cgtttggaaa agttgatctg tccatcctgt cgctaatttt tgggttcctt tcgaataaag
     4141 cctgtaatgg atcgaaccct tttttagttc agttgctgac caattggccg tgacttggta
     4201 cgatgcagaa gaaaggagct ttccccactg actatcggct gttaagtttg ttggtaaaac
     4261 cgttccgtct agcttgtact gctccaatac ctttaaagca acaggagaaa gcaatccgcc
     4321 ttgcgcatct ttcccactgt acacgatatt ggctgtctga ttcaatccac ttgtgcttgc
     4381 tggagtatga gtcagtattg ctccttgtgt aacgtttttt cctgtaaaat agttgactac
     4441 gcgatcaaag cgactaaatg gcaactgatc aaaacgtcct tcagtcagcc ccatcgtcaa
     4501 tgtccgacta ttggtagagc ctggaaggtt ctggttattt ccatccacgt attctgtggt
     4561 atcagcaaat gtattatatt ttcctgttga atcacttgtt gagatgtaac tattccacgt
     4621 atttatctct gcacttgcag aaaatgcata tgctcctgga ttcaagtttc cgccagttaa
     4681 tttaggataa aatctacctg cagctggagc taaaatcgaa ttgaacatac gatcttggat
     4741 aatattattc gaatcataaa aatcaacaga aaaaacggcc tttttagaca tagaaggttg
     4801 taatttaccc ttggtcaaaa ttttgtttgg actcgtatca tatttatctc cgttttctgt
     4861 catcttcgcg acattagctg taacatcaaa ttgaagcggg atatcgtcca cgttgaattc
     4921 gcctcgcgta ataatagcaa taatatctgc caatagagta tttgtatccc acgaccccat
     4981 cccaaaacgc aaggtatggc ttcctgctga taataaacta ccgctagtaa ccttgatggg
     5041 ggcaccggca atatacaagt aggcgttgtt ccagtcaatt gcttttaata catcactgct
     5101 tgatacatgt tccggcaaat tgatgatcgc cacaaatgca aatccttgtg cagtgatatt
     5161 caccaaatca agtacatttg ttagtttcac atggctttcc caatgaaaac ggaccgtgcc
     5221 acctttccaa ggatcatttg aaccatcgtt caaataagta gcagtcaatt taggcaattg
     5281 gttagcccca gttagttgga caagcttccc gccattgact gcactacgtc caccatttgg
     5341 aggtacgata ttttcatacg tgtatggatc ataagaaatg gcaggaggcg ttgctccaac
     5401 agcttctgcc gaaggtgaaa agataaatat acttaaaaca aaaagcgaag taaaaaagaa
     5461 ggagcgaaaa aatgaaaatt tattttgtag caacatgttc atcctccttt tcctttcttc
     5521 tccataacaa caaccacgca agaagccata attctatacc aatgatcaaa taaagaaaac
     5581 ggatcttttc ttcattcgtc tccgggaata cgccatgttg attagattct ttataaatcg
     5641 gtagctgtgt ctgctgtttt gatgcgccat tgactgaacc acttggatgc ggaactggac
     5701 ttagatcata aaatgtaatc gtacctgaac ttttggtatc tgcttggact ggctcttgcc
     5761 agctgaagaa cagcaaactt gcaataatcc ctcccaataa gatcgatgtt ttcaacgaaa
     5821 tcacctcatc atgttgtttc tccttttttc tttttcttgt ggacccacaa aaacagcaaa
     5881 atcaaccata acactagaac tgcagcaacc aatcctgcat aaagccatgg gaaagattgt
     5941 tcttgtactg caaaaacgga attttcattt actttttgtg ctttttcttt tttgatcgta
     6001 aagttttgtg taaatcgcca ttcattactg tcgcctgatt ttgctatgat ttctgcgcgg
     6061 taatcgccgg cttttgcttt atcaccagct aaatcaagat tcaagtgaaa attcgaattt
     6121 ggtgcaacta aaaattcatt agacgtattt tcaatataaa gctgattttc ataaaagatc
     6181 ttgctcgtaa atatcaaatt gctgataatc ctaggctgag gattttggaa acgaagctga
     6241 acagttggct ttgcgttgac ctcgtccaaa tctcctgata aaagtttcag atccggttct
     6301 atcgtctctt gactttcttg taaaacgact gcgagattat aagcaatacg atttttgatt
     6361 tgtgctcctt ctgtagcatt ttctgtttca gcttcttctt taggtgaaac atgaataccc
     6421 gctaatacac gtcccttaaa gctttttgct gggattttca cttctatagg tatagttgtt
     6481 tctgcatgtg ctgatacatt gatttccttt ttcgcaatga gcaaaacgtc acggacatca
     6541 aacggcaatg tctcatctaa cgttgggttt ttttgactat aatcgatcgt tccaccatca
     6601 ctcgttaccg ccggattgac agagacctca aatgttcgtt cttcatctga agaattgctg
     6661 ataattactt ctagtgtctg attttgttcc ggtgccacta ataaatcaaa ataaccgatt
     6721 gctttatcta tttggttctc tggaaatacc ggtttgattc caaagtctcc tgctgaatcc
     6781 tttgcttggc tgattgacgg aaaaatgaaa aatagactta aaatagaaaa aaacgaaaca
     6841 aacagtcgtt tactttgatt cattgtccac cacatccttt caacaacaac tgcggtaaaa
     6901 aatccttcac ctatagtatt ttcaccgcag tttgttgaat ttatttcata ctctatgcta
     6961 ctgggccagc cacgaggttc caagtcaaat cagccgtgaa tgttccagct gctgctttcg
     7021 gcactttcag tgtgatattt gctggatcat aaacagaaag ccatgttccc agaccttgat
     7081 cttttgctgc tgtaaatatt ggtgtcgctg cacttgcttt actcaaccca gttacagttt
     7141 gactagttgg tccagtggat tcgttatctg acgtactttt aacagtggat gttccaaaat
     7201 ctaattcagc gccttgcaaa acttgtgaac cgtttacaaa atctgaaacc gaaactgtca
     7261 cgttccaccc ttgtgcttgg ccatctgcac ctacacctcg acgatctgat acttggagat
     7321 aaggtgtatc gttttttgaa gcgtctactt gatacgtttt tgttccgctt tcaatctcgt
     7381 gtgtaccaaa tggcaattca ggaactacat ccaatgttaa agaacctgtc tggccagttg
     7441 ctggattttc cggatctgct ggctctactg gttttgaagg atcagtaggg ttaagaggct
     7501 caacggtatt tgttggttca gtaaaggtaa tatctccttt tgaagtcgct tgaccctcag
     7561 caaaagctgg tgttgccaag ccgcctagaa gaagtgttga aagtaataat gtcccaatca
     7621 tgttcttttt cataataaat cactcctttt tcttagtgac ctaagtatag tagagacagt
     7681 atgcaaaaaa tcctcatgaa ttcatccgct tacttgaaat tatgaccctt tttgtataaa
     7741 agagaaaaaa acaataaaat cgtttacatg aatattcttc cttttttatt aagtatgaaa
     7801 caatatttca attttatttt ttcgtttcaa attattctat tagtgacatc cttgaactat
     7861 aaaaatagga gtacggcatc atctgccgca ctcctatttt tttcgtcatt tatttcttaa
     7921 ataaaacgat tctcacgcaa ttttattttt ttgatatctt ctactgtttg tgttccagct
     7981 aattgcatga ccatttccaa ttctgtttta aagaaatcaa acacttgatg gactcctgta
     8041 cttcctccta aagacaatcc ataaataacg ggtctgccaa tagcaacaag atcggcacct
     8101 gatgcgattg ctttgaaaac atgttgtcca cgtcgtacac cgctgtcaaa cacgattggt
     8161 acacgtttat ctactgcttc tgccacatat tgcaacgaat caaacgcagc aggcccccca
     8221 tctaattgac ggccaccatg gttagatacc caaattccac cagcaccaga ttctaatgat
     8281 cgatagacat cttcttctga ttgaacacct ttcacgtaga ctggcaaatc agaatgagca
     8341 gcgataaatt ctacatcttt tggactaagt ttttgtttgg aagatttata tacagcatcc
     8401 atcgtttggc ctactcctga ttgataagcc tgaacgatcg gcattggcag cgggaaggta
     8461 aacccgtttc tgcggtctgt ttcacggttt ccgccaactg ttgcatccgc tgtcaatacg
     8521 attgcttttg ctccattacg cttggccact tcaagaatat ccaaattgat tccattatct
     8581 ttgctcatat aaaattgaaa ccattgcggt gctttctccc cgcctgcttc tcttatctct
     8641 tctaaagtac aagaagcgta agaacttgct gtatagatcg tcccaaaatc tgctacacct
     8701 tttgcagaag ctttctccgc cttcacatga gctaatccat gagcagcaac tggtgccatg
     8761 atgattggtg cagtcagcat ttcttcgtca aaatgtgtag ttgtatcggg taattctaca
     8821 tctcttaata catgaggaat aatcaaacga tgattgaatg cccgttcatt ttcttgataa
     8881 gtaaataagt cgccagcacc actgctgatg taaccataac caccagctgg aatcacttgt
     8941 gaagccgcga tttctaaatc ttccatattg ataaaatcta ctattccttc atttgtacct
     9001 gcttgatatg ttttttccat ctcaatgcct tctctctata aaatttgaat gatgaacaat
     9061 atgatagcgt ttacactttt tatattactc ttcgacattg atacaggcaa ataatctgtt
     9121 tacatttcag ccattccaga tgtcactact ctggaagaaa tgattgtaat aaaaatagat
     9181 aaaaaaccga tgggactttg acaaaagtag tgtcaaagtc cctttttttg aataaacggt
     9241 gttcaaaagc cattttctcg gtattaagtc aaatttatgc aaaaatgtgg aaagccattt
     9301 tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc tcgataaaac
     9361 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat gaggaactat
     9421 tttgccattt gtctctctaa tactcaggtc agaacgttga cttcacagtc tcttccttaa
     9481 acggtgttca aaaacttgca tctgcggtaa taagcccaac caaacgaaaa ataggaggag
     9541 ctatttttgt ttggctgttc ttattacttg atgcagggca ggtttttcac gacctctgat
     9601 tcacggtgtt tctttttcat caacattttt acgaaatact gtggaagact attgaaataa
     9661 gtgtggattg tttctttgtg tttcaatcgt ttcttgccat tcttcattta attttggatt
     9721 accgttttct tccttgaact cataaggtcc taattcaaaa atcccagaca gtatttgatt
     9781 cacgatttca tatagttcag tgtgatcaga cacatgattc ttgaagatca tctcattatc
     9841 accacgtttg aacctcacat tcgggctctc atgatgaacc tctatgcatt tttctccttt
     9901 ttttaactga cggacttctg aaaacatctc ataaatatct tctccagtaa aaccttcagc
     9961 aagcgccaga ctgtccacaa gaccgcctac tgtcagttca gagattattg cttgattgga
    10021 atctaatttt aatgtatagc ttgtcgcttc tactacgcca catgcattgc aaccgccttc
    10081 tgatagttcg ctttttctaa tcaattgaac ttctttcatt ttctcttcct cctcttaatt
    10141 ttctacttat gtatttttat cacctgacat aggtgactcc tatcaacgct atcatgtgct
    10201 tttttcctca gaaatcctat ttatccattc catctatccc ttattcttct acttttcttt
    10261 ctctagcata taagagatac gcatagtatt caagcaagga ttgtcgatat aaaaagaaca
    10321 aatactgcta ttttgatttc ttttggctat attacatata aataaaattt atcatacctg
    10381 ctctttttga ctatatcatc atctgacttc tatacttcgt ttgtattaaa acttaggagg
    10441 aatcattcat gcttacaggg ttaaacttat taaataatcc ttttctaaat aaaggaacag
    10501 cttttacaaa agaagaacgg gcaaaatatg gaatcactgg tatgcttcca agtactgtcc
    10561 aaacactgga acaacaatct gttcaagctt atggtcaata cttaagcaaa caaacagatt
    10621 tggaaaaacg tatcttctta atgaatctat tcaatacgaa tcggacattg ttttataaat
    10681 tgatgggcga acatttagtt gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt
    10741 caatcgaaca atacaacgaa attttcttga aaccgcaaga tgctgcattt ctttcaatcg
    10801 acgaaccaga aaacatcaaa gcaagtttga agaacgcggc agatggacga gacattcgtt
    10861 tgattgttgt gactgatgca gaaggaatct taggtatggg cgactggggt gtgaacggtg
    10921 ttgacatcgc tatcggcaaa ttgatggtct atactgctgc tgctggtatt aatccagcac
    10981 aagttttgcc tgtatcgatc gacgctggta ctaataatga agaattattg aataatccct
    11041 tatacttagg caatcgtcat gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc
    11101 aattcgttca atcagctacc gaattattcc cagaattgtt attgcattgg gaagattttg
    11161 gacgaggaaa tgctgcagcg atcttagaaa aatacgaaga tcaaatcact actttcaacg
    11221 acgatatcca aggtacagga attgttgtcc tagcaggtgt attaggcgga ttgaatatat
    11281 ccggtgaaca attgaaagat caaataattt tgacatttgg tgctggtact gctggtgtgg
    11341 gtatcgcaaa catcctttta gatgagatga tccgtcaagg tgtacctgaa gaagaagcac
    11401 gccaacattt ctatcaagta gataaacaag gtttattgtt tgaagataca gaaggattga
    11461 ctcctggtca aatcccattc gctcgcaaac gctcagattt cactaatgct gatgaattga
    11521 caaacttaga agcagttgta aaagcgatcc atccgacaat catgattggt acttctaccc
    11581 aacctggagc attcactgaa gaaatcgtga aagaaatggc tgctcataca ccacgtccga
    11641 tcatcatgcc tttgtctaat ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag
    11701 aatggacgga cggaaaagca ttagttggaa caggtatccc tgctgctgat gttgaatata
    11761 atggtgtaac ttaccaaatc ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac
    11821 ttggtttgat tgcttcaaca gctacacgag taaatgctga aatcatttct caagctagtc
    11881 gagcattagg cggaatcgtt gatgtaacaa aaccaggagc agctatcttg cctccagtag
    11941 caaaaatcac tgaattttct caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac
    12001 aaaacttgaa tcgtgaagaa atcacggata tcaaagaagc agttgaatct gctaaatggg
    12061 ttcctgaata taaatcttta gaagattaag atcgttatag taaaagaaca tacagaaact
    12121 ttgtatgacg tctaaaaaga aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg
    12181 ctcttttgtc gcttttcttt ggcttttttc atatgataca gaataagaaa ggatctgtaa
    12241 aaatggcaca aaaaacaaca actgcaactg cagttgagga aaagaaaaaa gaaggttttt
    12301 ggtccacaaa aatcaccggc gtaagtttgc cactttattt gatcatggtc cttgtcctga
    12361 tcattgcaat ggctttaggg aaattaccga cgaatatgat tggaccaatc gcagttttgg
    12421 ttattttcgg taatttattt cattttatcg ggacaaaaat ccctatcgtc aaaagttatt
    12481 tgggtggtgg ttccgttttt gcgatcttcg cttctgctgc gattgctaca tttgggatct
    12541 tacctgaata tgtagtgaaa tccacagaaa actttgtcag caatatgggc ttttgggact
    12601 tttatattgc tgccttgatt actggtagca tccttggtat gaaccgtaat ctattgatga
    12661 aagcttctgt tcgttttatt cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg
    12721 taggactcgt gggcatgctt atcggaaatg gttttgcaaa ttccgtttta tacatctcat
    12781 tacctgcgat ggctggtggt gttggagccg gagccgttcc tttatcaacg atttatgcaa
    12841 atgttttagg tacggatgct tcttctatca tttcccgcct gattcctgcg acagcaatga
    12901 caaatgtctt agctatcatc ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa
    12961 aattcaacgg caatggtaaa ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa
    13021 ctgtgaaacc ttccattcca cagttaggtg ttggtttgat ggtatcattg actttcttca
    13081 tcttaggaca aattggtaac tattttgttc ctaaagtcca tgcttacgca tttatgatta
    13141 ttatcgttgt catctgtaaa atcgccaacc tattaccaga gtattatgaa gatgcagcta
    13201 tcatgttcaa taacttgatt gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta
    13261 ttgcgttgct gaatctgaat gtattagctt ctgctttgac atggcaattt gttgtcttat
    13321 gtctgacaag tgttatcgcg atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt
    13381 tatatccaat tgaatcaact attactgctg gcttatgcaa caactcgatg ggcggaacag
    13441 gaaatgtagc cgtcttatct gcagctaatc ggatggaact tattgctttt gctcaaatgg
    13501 gcaatcgact tggtggtgcc atcgttttga tcatctctgg tttcttgatg caattacttt
    13561 cttagtcaaa aaaacaaaaa tacctgatca tcaagtatcg gcttggtgat cgggtatttt
    13621 tttgataaag atttacagga ttttcaaaaa tcttttttgc attttatcgg aatgataacc
    13681 gatgtgttca taaaattggt gtgcttctac tctgtattct gcagaattaa gccttattgt
    13741 cgttattcct cgttttttcg cttcttcttc tacgccagtc atcaaagcct ttccgatgcc
    13801 tcttttttca gcatatttag acacagcaag tgccataata ttgaacattg gatcgctaaa
    13861 aatcgattca taggactccg cttgtacata gccagctgct tttttagaag taggatcttc
    13921 ataaaccaag aaaaaatttt tctctggctc cttcagcagt ttcttcattt gtctcaccgt
    13981 aacggttaat ggtatatcat accctaattg atccgcattg atttcacgta gtgcttctgc
    14041 atcttctaaa cgcatttccc gtaacatcca aatcaccagc caatttctga ttttttaaaa
    14101 tgaatttttt tatcatagta tactcttttt ttcatttaga acaatatgtt tcagtgattt
    14161 tgacttgcgt tagtctagat tctcctaatg tttgtaagat gacaaaggca ttgtaacaat
    14221 tgaccaaaag aaccacgagg acaattactt gaaaaataaa ggtcactttt tttggagaca
    14281 aaaccctgct aaatcttgct ccccacaaac ctccaataac agcagcgggg ataacataaa
    14341 acaacataga taaatcaaag cgcataaagc tacttgttaa agcaatcgtt accaattttg
    14401 ccagttgaga aaagaaaatc gtagatagag aatatagtgt tgcttctttt attggcaatg
    14461 caaacatcag catcaacaaa gaaacattga ctggtccacc accaatacct aaaaaactag
    14521 ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt
    14581 ttggctgatg atgcttggtg tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa
    14641 tttggatcag ctgcacatgc ttctcgtttt caaataactg taaaaaaact tcgaatacaa
    14701 tgttgccgac tactcctcca acaacagctc cactagatac ccaaccgaca atctgccaat
    14761 tcagtgattt cccgcttgat acttgtcgca cagtcgaaac aatggacatc gtgaataccg
    14821 caactgttga gtaaaaagaa atccctgcaa cagaatgggc gccgataaga tcaagaatcg
    14881 gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc aactgtgttc gccagaacga
    14941 tcaccacaaa ataaatcaga ccaatcatct tgtatttcct ttctcttgtt tatatctttt
    15001 agcgattgtt actcttttct tttattatac agtaaaagaa acaaacgagg cttcgaaagc
    15061 ttttctactt tttaaggaaa tttagtgcta aacgaaaaga ggttgtgaaa aagctgtcct
    15121 gcatcaagta ataagaacag ccaaacaaaa atagctcctc ataattttcg tttggttgaa
    15181 cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg
    15241 ttcagctccg agtcacaagg aacaatttca aaaaacagct tctcatgttt gttgaatatt
    15301 gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagagac tgtgaagtca
    15361 acgttttgac ctgattatta gagaaacaaa tggcaaaata gttcttcata tttcgccgtt
    15421 tttcgagcga atgccgaggg gcagctgatt gaacatcgtt tatttggaaa aagggattgt
    15481 gacaagacgt ttgtcacaat cccttttttt atatcttatt aattcattct tttgttctat
    15541 tcttcttctt ccgccatgaa tgagaaaccc atatcaaaga actataaagg ttatcaaaac
    15601 agcgtttatg actaaatgaa ttagagttat ctatcactat ttttcaagcg atttaaacca
    15661 aatggcatac aatatggcat acaaaaactt caataagccg tgggggtttt taaatttctt
    15721 cttgttcttt aaaatcaatg agtggagcta cataatttga atccactttt tcaaacgtca
    15781 ctttttgcat cgacgaatgt tttctttccc aagataacgt aaatagattt gactctggac
    15841 ttataaagtc tacttcacct tctagtatta ctttcttatg caaatattcc tctaataaat
    15901 atagacttgc taaagcatac aacacatttt ccaaatttgc ttccttataa tttgaagaac
    15961 gaccatgttt tactctgtta tatattttcc accaatctgg tgtatcttca ggccacccat
    16021 ctccaaaagg ttttagtgaa gtcaatgtta aagaattcat aaccctaaca ccattatcca
    16081 aacacattaa ttcgctagat ttaataacag ctttaaaatc agttatgctt ttattgctac
    16141 tgctatcaaa tccgcaaagt tctttgaaaa taatctcaca ctccgaacca atcgctagta
    16201 gtagtttttg atattcaata gagaatgttt tgaaattatt ctcatcgagc atcacaaaat
    16261 ctgtggtatt aataaactca ttttctaaat gtagataata cttccaataa aaatttgtaa
    16321 actctgcttt gtccattaaa tcacctcttt tgaattataa caaaaaaaac acgtccacaa
    16381 cagacgtgtt ctttgatttc tacacaatca gcttgccaaa accatccaat cagtcctagc
    16441 atgcctgtat taaaattgaa cgttcatcat ctatcatctt tcttagtcca gcatgtttgc
    16501 aaccacacct acaattttca gtccaacaat agatttcgac tagctaagtc ttgcaaaatt
    16561 caggcagcta acccttcatt tactacctta attatactaa atacccacaa cgtttacaag
    16621 tgtaatcatt ctaagaaatc tgtttagcta tgatcgttgt ataatcttta aacgccgttc
    16681 cttcattgat tgaaataatt ttataactct tgtttttcca agctactttc atatcgtttg
    16741 ttagttcata atcttgatcg tatcgaataa taaacgtcaa cgttccttca agaatggtac
    16801 ctacactagc aagcacgtct tttagcattt gtgaacgcac attagcccaa cattcaaata
    16861 cagtttcttc actatccacc atctgcccat cttcatcttt tacccgtttt gtctttttaa
    16921 acttgactct ctcagtcaat tcatttacat tcttaatgat tggcataata agcacctctc
    16981 atttgttgta ctaatgcttg taccgttact ggtatccgtt cgctgctggc ttcttgacgg
    17041 ttcaaatacc aatgttgagt aagtaatgac accgccaaat caaattgctg ataattatcc
    17101 atatctttat ccgttgcgct actatcaata gcacttttta tatattcgcc tgctgtatca
    17161 atcaattgtt gaataaagtc atcatcaata gtgtgatcga tccgcatact tttttttatg
    17221 ctatccaaaa ttaccatttt atctctccta atgaaaaaaa ggcagtggga taaaccgccg
    17281 cccaatattt ttctatactt ttggtgttac aactggcgta aactcaatat acactgaagc
    17341 attttgatca attttcttat aatcgttgcg aacgatcact gcaagtcctt gtgagtagta
    17401 atcgaatttt tcccattgag tcgttacttg atttctacga gcaacaaaga tagattgtgc
    17461 aatatccccc ataatcatag ggaatgttcc agctttagga ttggcaaaca atgtatctgc
    17521 aatcaatact actggcatac cgaataatga tttaccacta ggtgctgtca catcgggttg
    17581 taaaatgtaa cgtccatctg aatctttcaa tgtatctagg tggtaatatc cgctttggtt
    17641 tagaattacc attttattta atgctgggtc taatgccaca ttgtatagtt gtttcaaatc
    17701 gtccaacgta gcagccgttt tcttagtgaa tgtttttaat aaatccatga tatgcttatt
    17761 gtcagtgttt tctaccaatt tcgctaactg atctttgacc tcttgtacaa tattcactgc
    17821 tgaatcttcc acaacctcat ttgataaggc aatcttacca gcacgagttt ctactttata
    17881 atcaactgaa gtaaacattt cagcgtcaat atcaccaatt tcagcaagtt cagcttttgt
    17941 tgctaacact gcttgttggt tagttgctac tggatattta ccttgtccat ttgatactgt
    18001 ttttactgtt gcatattgag ctaagttata atttgaacgt ttcaaatcaa agacttcacc
    18061 gattacttct tcgggaacaa ctgccgctgc atttacagta gtgactccat cacgcacttc
    18121 gccttgtgaa cggatatatt cttcataccc acgcaattct gttttttcgt tatcgattaa
    18181 agttttttcc atgttattac tctccttttg tgctttagaa gtggattcta agctattgtt
    18241 tgtttgtttt gcttggttgt tactcataaa cgattcgtaa gaacgcttat ttacttggac
    18301 atttgatgaa tcataagcgg gaacggtcac gacactaatt tcattaagtg ctttgatttt
    18361 atctattgat cgttcaattg tgccatcttc ttttttgtcg aatgaatcta atccaagcac
    18421 gaacccaaag gacatagaat caacaacccc ttttgagata ttctcataca catcatttga
    18481 ataggtcgtg tcggttagct ctgcttcaaa atgtaatcct gtatcatcga cattcaattt
    18541 caacgtacct gctttgacac tagctagtgg ctttgaataa tcgtgtccat ataataagaa
    18601 cacatttgat aaatccacct cttttaatgc ttcgggtgta atcacttcta caaagccgcc
    18661 taaatctttt gacggttgcc cgaatttcaa cgcatagcct gaaatagttt tgccgtctgt
    18721 tggttgtttt tcattttctt tctctacttt gggagaatcg gctgttagct cagcttcttc
    18781 tgtcagccgt ttttcctttt cttcttcatt attcaactgg tgtaactcct tcctgttctt
    18841 ttaattgata tttctccaac gtatctaaat acgtaaagtt caaacttgct agtaaacgat
    18901 cgccaccatc catggggggt agccccattt tcgaccgccc ctcattaatc gtgagaagtg
    18961 accctttgac ctgatccaat acatttttta cttttgtttc aggatcagtc tctagtaact
    19021 gatcggaatt aaagcggata ttggtttcaa gtttcgtatc taattcactc acaaacacat
    19081 taaagtaatg aatcagcgta ctttggatat actggagatt actttgaacg gtgctagaat
    19141 gttcattttc aacgcctaaa cgctcaatcg gtacaccaaa cgccttggca atttgttttg
    19201 tgttccaatc attggaattg ataagcttca aaacatcggt gtttatttct aatgttttgt
    19261 agtccattgt ttcatcaaga atgatcgttc taagagcatt atctccacta cttgaaccgt
    19321 tggcttcttc aaacttttca cgaattgcgt tctttgctga tccgtctaaa tctgacttat
    19381 gaaccttcaa aatacctgac ccactgacac cacgagtaaa gaaattgtgc aatgtgcggt
    19441 tgccagcttg ttgaattttc agttcatcac gtaacgcata aagtggtggt aatcctgtta
    19501 aaccatcttg ggtgaaatat ttgaagtgca aaatatcgct agacttcaca cgtctttttt
    19561 tatcaccaat ctgataagac aacgtaccat tatctaattg agtaaccgtt acacttgagt
    19621 taggtaataa gtgaatttct tctacctttt caccgttgcg tttaatctct gcaaagctat
    19681 taccgtttag caacatattg accgccaaag caaactttaa atgccaacca tccatttctg
    19741 aattaggttt ctcgtttagt aacttcacca gttcattatc agcttgtggc atattgtttt
    19801 tgaccaattg aatcggactt gaagcaatat cactagcaat aattcgaacg gctgcaaaca
    19861 catcactatt tcttaatgca cgaacgcttg taaagctggt ggtgtaatca tccgattgga
    19921 ttgataccac atgttgtagg aaaggatctc ccgttgtatt ttgtctcgct ttaaaaaatg
    19981 ccatctttta acctcccttc tgttctgtat ttagcatgac cgctacccca ataagcacta
    20041 tgcccagcgc taacaagcca tagtagacat ttgttagtaa ggtgatagca accaacacac
    20101 acaccaatcc aagaagtaaa agaacggtat gaatgtaatt agaaagaaaa ttcttcactt
    20161 gtataaaatt cattgtctgc tttcgctcct tccgttgtgg tgaagtaatc cattgcaaac
    20221 acataggcat tgattaatgc agctattgga tcaattttgt tactgttctt tgatttatta
    20281 atctgaatgc cgttgttatc cgtttttaaa atagcgttgt tcactgcatg ggtgagaatc
    20341 gtatttttat tgtgaataac gttgccttca taaacttgtt ctctaaaagt tcgggtcgga
    20401 acgtttagag taatcgttcc ttgtcttact tctaacattg ggtagttggc tttttcagct
    20461 ttactgataa gcgaattagc gttatacggg tcataacaaa tccccataca ttccaaatca
    20521 ttttcttgaa tcaaatcttg aataaactga aatacttggt catagtccac gattccactt
    20581 tctaattgag taatggaaca ctcaccagct ttttctagtt ctcgataagg caaaccgtcg
    20641 cgcttttctt tgtcttgaag cccatatttg gtggctacaa agctatgtga atcacaataa
    20701 agttcgccgt tatctagcgg tacgatccat gaaacgctag ttaaatcatc tgttttagat
    20761 aaatccactc cgatataaac gggcttgcct gtaatatctt gtggttctac ttcggttgcc
    20821 tgccagtctt cagcaatcat atagctgtcc tcactcgctt gtctccataa attgaagttt
    20881 ttcaccaata cagaattaag gttattttgt ttcaaagcaa ggttcacatc atcttgaatg
    20941 gttggaatca ttactttttt gatttcttca ctttcaaaaa tcggattggc tttgatccat
    21001 ttctcttgat cgtgaatttc ttcgggatcg tccaattccc atatcgctat aaagtacctg
    21061 tcggcttgtt cttcgccttt tagcacacga tctaacaata agtattcctc gtacatagga
    21121 acgtttaaat caagcccagc ggtactaata acacacaata agccgttctt ttgttgggtc
    21181 attcctgact taatgacgtt gtacgtttta cgagtttttg cttcgtgcca ctcgtcaaga
    21241 attgcggttg ttggtgcaaa cccatctagc gtgctggttt cactagccag tgccatagcg
    21301 aacgaattag agggcaagtg ctgaatctta gagttcataa ttttcagttg tggtcttaaa
    21361 aacttactag acttgaccac gtttctaagt gaattagaca gcatatcata gcccaattta
    21421 gcttgtttca aagcgttact tacaaacaat acttgccttg cttctgctgg ttcttgttcc
    21481 ataatcaacg cattggcagc catgccactt gcgatatacg ttttcccatt cttacggctc
    21541 atactggtaa acgctcgatt gaatcgcctg taattccctt ctttagtacg ccagccataa
    21601 agactgccta gaatgaattt ttgaaatcct aacatcttga ttgctttacc gtccgtagat
    21661 ggtagcaact caatgaattt aatcgctttg gtggcttgtt cttcatcgaa cacataaggg
    21721 aagctatctg acttagaacg ctccaaatcg cttatatgac gctcacacgc ttggataatt
    21781 ttcttgggtg caatcagctt acctgatagg actttctcaa tgtaatcatt catgaattca
    21841 tcaactcttt gaacgggtct tcgggttcat catttttaac cttgttcaaa gctagttttg
    21901 atcgtgattc taaggtcata cctagtgagg tggcttgttt tttcaaatca ctcatagctt
    21961 gtgattgaac ccgaaccgct ggattggctt tctttactcc gttttcgcat gtgataaacg
    22021 tacctgattt tcttatctcg gcttcggctg ttttcattcg agcaaaggct aaacaatagg
    22081 ctaccaatga accataatct gcttcactca atggataatc tttttttaat gttggtacga
    22141 tacgattcca ttcacttcta gcgctcgctg ccatccagtc agggggcgtg atatttatta
    22201 atggttgctg cttgaacaat tcttctctag cgtccacacg gattgctttt tcttcacttg
    22261 atatattgcc ttttgtatcg tgtaatattt tagggtgtcc catgttgtcc tcctttcaga
    22321 caaaagaaaa aggaacgtaa gcaattaagc tcacattcct tcgtttttcg atcagaattt
    22381 ttctttatct aatttatcta tataaatgat ttttccgttc tgcatttgaa tggtcacttg
    22441 accaaaatga ggtaactcaa tggtgcgctg tttccctgtt ttatcagtaa taacaattgt
    22501 atttttcaat aaatctccta ctttcattat atactttttc acttgtgatt acaagtgtaa
    22561 tcgttatttg taattaccct tatgcctttt cacaacggtt atttgtacca aaatgagtag
    22621 gcatcgattg tcagtaagtt tcacactatg cccccttaac tcaaagggta ctcttttctc
    22681 gtctttgtct ttcattggct gtcttggaat tatggtgtgc atggcataaa gaacgaaaat
    22741 tagtttcatc taaccgtcta ttccaatcat cttttagttc aattttgtga tctacaacat
    22801 cagctttcct tatcaagcct tcttctaggc aatcctcaca tactggatga tttaaccgat
    22861 acaagtatga agcctttcgc catgttctac tttgatagaa ttgaaaatat ttaccttcgc
    22921 tttgctttct ttcttcacgc ttaattgccg tttgctttgc tttatgcttc ccacaatact
    22981 tctgcctata atcaatcaat gttttacatc cagcatgatt acattgtttt ttaactgcca
    23041 tagattacca accaaagcga tctcgttcgt cagaatcatc ttgttcccat tttgatttgt
    23101 attcaatata aggcgaaact atcttcaagc acgttccgtc tttaaaatta actttagtga
    23161 ttttatcttc ctcaaaacta ataaagtcta catcttcttg agtgtttgaa tactcaatgt
    23221 gtttctcacc atcgaagtat ctaatacttt ccatatgcat aattattcta cctccaataa
    23281 attagttatc aattcatcat ctgttagtag caaagccaag aacttatcat ggatgtttaa
    23341 aaacaaatta tcctcattcg atgtaagctc tcgttgtctc gctcgatcat ataggttaag
    23401 cagacaatta aattcatcca ttagctcacc tgtatcatca tagttgccac tatctaccaa
    23461 tgatcccact actagcgatt gacaccacgc tactgactcc tgcagccaat caggttcttc
    23521 ttccattatc tttaatactt ctggatcaaa tgtggaataa tcttctgtta ccaatcccac
    23581 actaaatgcc agcgtgttca ttagaaaccg atccatttca tctgtatatt gaaagttcgg
    23641 tgctttgttt gttgaaataa tattgagcat ttactttcct ccttgattcg taacacgtgg
    23701 taacacctac gtaacaggta cccgttacca ctcaatccca acggggacaa gggttctagc
    23761 gttttggtaa caggtaacgg gtaaaatcca gtaacattat tccccagcat attagctaac
    23821 tatttcaaaa gttttaagga aataaggtgt taccccgtta ccaaattgct gtatcccttg
    23881 gggcagtaag ggtaaactag taacgggcta ggtgttactg acccgttact acccgttact
    23941 ttttgtactt tccagctcga tttttatttt cataggcatt tgacttattg taaaatcctc
    24001 gtcgtttatt tctttttggc agttgcttcc gttcccaacc aggaatactg ctaataatca
    24061 aggcaatctt tttagtttct gcatctgatc gaccattcaa taaatccttt gcttgtttat
    24121 caaaaacagc ttccaaaata tcagcggtga gtacgctatc aactaaataa aactcttgtt
    24181 gcatagtagc tccattatag aaatgctgta aatttttctc attacattta ctgtcaggat
    24241 agttctgata ataagaacgt ctcgcccacg ctggagcttc ttcccattta tacgggattt
    24301 ccatttccaa atatttagtg attgcttctt taattggatt ctccatcttg gcatcctctt
    24361 gatagtcttt tgcaatttct aagacttctt tatcttcatc gggactaaaa tagattcgtt
    24421 gtcctttatc aaaaagcacc tttgcttcag ctaacacttg aagaaaatat tcatcctttg
    24481 ttttaaacgg attcatcttt ggcttattct tacaaggcaa aggataaaaa cggcggtttc
    24541 ctgtatcatc tttcaaatac tgcccatcat tcgacgttcc aataaataca cattgccttg
    24601 cccaagctat gacattccga ccataaggag gtctaatttt atcgatcttg gcactgatga
    24661 agttttttat ctgttcaatt ttagttttgt tcattgaaga taattctcca agttcaataa
    24721 ttacattgcc ttgaagctgc atataatcat ctttcttttc acctagcgaa tctaatgaat
    24781 cggtgaaaaa atcatccgtg taaagtaatg ctggaacggt actcttacca atcccttgcg
    24841 gtccatccaa aactggaacg atctcaaact ttattcctgg tttatagata cgtgcgactg
    24901 atccgactat ccaacgtttt gttacttctc gtgtgtaaag gttgtcgtct acccctaaaa
    24961 ggtcaataaa cagtgtttcc agtctaggct gttgatccca tttaactttt tcgatacgtt
    25021 cttttactgg gttgaaaaca ttctttctag ccacatttac tactgctttg tgtattttgt
    25081 tgtcatcaaa aactacattg tagttttctt caatatatgc cgttaaggca tcctcaaaac
    25141 tgtcttccca ttctccagcg gtacgattga tccaaggaga atcttttaat aggtgaatgt
    25201 atccgctaaa ttcgttataa gcaattgttc cagctaaatt gctatcattg gttaaaatca
    25261 attctgcatt cacaacactg ttacttttaa tctgatcgtc tttattgcat cttaatcgtg
    25321 acatccaggt tttcaattca atgatattgt cttcacgctc gctggcttcg ggaaagtcta
    25381 aaatactatc taatttatgc ttatgagcca tgagctttcc ccctcaaatc ttttttaatc
    25441 atcgatcgaa taatttgatt cacttcttta tcttttagcg gtggttcatt gtacgcattt
    25501 gctgtataaa tcaattctgc taaagtttga ttatctacac cgtgccatag taaccaaccg
    25561 cataacttag ttaaatgatc gtttctacta ccttcgccag cgccttgaac aatcatatct
    25621 aatttttttg ctgtccctgt ttttgctacg ggtcggtatt gagtctttat ttcttgcggt
    25681 ttttttagca gtaggttcac tagccaatcg ggagctggtg caatattatt tagagatacg
    25741 ttttcaatag gttcatatgc tctcccaaca cttcttgaaa taatcatagt tggcgctatt
    25801 ggaactcctt tagtgataat atcaatcccg cttaaacttt tacctttatc atcacgcccg
    25861 aagatatcag caaatgcacc tgtttcttgt tggataatta caccatcagg aattttaaag
    25921 aagaaatgga ttcctttacc tggtgtgatt tccgtataag tagaaggaaa tggatcatac
    25981 attgaagcaa gttttttata attctcaacg ccgtttcgtg tttcagaatg ttgatctagg
    26041 tcaactacca ccatttgatg atcttcaagc attaacccaa tatttctagt tgcatcttcc
    26101 gtccaccatt cattaatttg attagaatcg ttagttgcat cattccaacc actagagcct
    26161 tttattggaa ttttagttcc aggagataag ggataaattg accatcccga cttttgtaat
    26221 tccgttgctg cttgataatt atttgccaat aaacctctcc tcctttttca aaatactggt
    26281 ataaatcatt cagacacctc catgagcttc aactgctcaa gttcgtattt caacccatcc
    26341 cgaacaaatt cagcaccttt taattgattc tgatacttta cccatgtatt aaatgttttc
    26401 acccttgctt cttgcatatc agtaatgttt aaccatgcgg ttaaaatctg attttttagc
    26461 tcgttgattt ctcgacaaat tattttgtgt tctgcatgtt ttttgagtac atacaaaact
    26521 gttagttttt cattgaatga atttccaaag taggttttcc cgatattatt ttctagttca
    26581 gcgatccctt gattaatttc ctctaattca aggctatcga gttggtttaa tagttttgtt
    26641 ttgtacatct acttatgccc tccagttctt aattgatagt cgtactcgtc ataagcagtg
    26701 aagagatata acgctaatcc tgcaaagata gctatttttg caattaatgg taataagccg
    26761 aaaattccgc cgattaaaat gcttcctata attaagtttc gtttcataat ggttccctcc
    26821 gattttttaa tatggtataa tcggaacaaa gaatatcggg ttttatccca attaagttgc
    26881 ctttatcctg tccgccaaga ctgttatgga taaggctttt tctttgttct cgatcatgtt
    26941 aatgctccta agttgtctgc aattcagcag aacgattata aattttctca ctcactgaaa
    27001 actgcaaccc atatttttct ttaacagtca ttaattctac tgtctcatct aaaataggtt
    27061 cacgatcaac caacatttca ggagtcatat ctttcttttt taccattttg gaatgaccat
    27121 aaatagttga tacagcttta ttggctatca cattcgcttt aatgtagtct actggttttg
    27181 gttcagtaat agcgtggctt aatttagtca ttgcttcttt ttggtgttct ttgtctagca
    27241 tgcggaatac ttgaaagcct tcaagtcctg tggactggcg tagttctttg atgatgtcga
    27301 aaatccagtc ttgaaatgcc tcggcttctt ttttacggct tttgaatgcc atgcggtaaa
    27361 tatttttttt cgttgatgat gtttacatct tgtgttctgc ccaatgaatc gatgacctta
    27421 ctagtagtaa ccccatcttt aggcaaagag tgaatggctc tagtaacctg tttaagacct
    27481 aacgcttccg caacatcttt cgccactgcc caccattcat cattaatatc gacaaatctt
    27541 ataatgtgtc cgttccaaat ttcagttttc attcaacgtc ctccagttca agccctagca
    27601 agctcgctaa caactcaact tgctgataca aaatttcttg atactcttca atagaaagtc
    27661 gttctttaaa ctctgcttgt actaaatcgt tcaagccacg caatactccg cttaccgttg
    27721 gttcttgtgg tagcaatttt tgatagtcca tttgttcgta caaacattca aacgtttctt
    27781 ctagcgcttt gccttccaag tttgctaatt ctgccgctgt gtcttctgta agaattgttg
    27841 ataatacttt ttcgattgct ttattttgtg tcgattccat tattatttcc tcctattaat
    27901 ccctttgccc ccacggcttg cgtttatttt tggtattctt ccataaagcg atcacattca
    27961 tcttgtgaaa ttcttacgac tccatcaact ataatcaact ttaaaccgtt aggaacatat
    28021 ttctgcatca tagtattgta tgaaacgttc atatacttag cagcttgtgc cttgttcata
    28081 tattttggaa actggtactt tttttctaat aatgaaagta acgttccttg agtttgctct
    28141 acttgttgaa caactcgatc gtcaataatt ttaaaagctg gatcatcaag cgtgagtttg
    28201 tcaattttta attccatatt tatcccccct cgttagcata catttctata caaagatttt
    28261 aaaaaaatgc aagaggctct tgaatacatt tctatacctc ttataaactc aatacaccat
    28321 catacctttt catttgcaag aattttaaca tacttttttg aacttttatg tttcacacat
    28381 atttaaatcg tgatatagta gagaaaatat atgaatggtg gtgaaaatgt ggctagaaaa
    28441 aaataccccg aagcagtgga gacaggacaa agaattagac tggctagaat tgaaaaggga
    28501 ctctcaatgg aacaacttgg aggtaaatta tccccaccgg ccagtaaagg tgccgttagt
    28561 aattgggaaa atggctataa tcttcccaat aacaccaggc ttaaacaact ttcaaaggta
    28621 ttagatgtat ccactaccta tctattaaac ggatcttata cattaagaga tatgcatatg
    28681 atgcccgaaa ttaagcgaaa tgtgcttgaa aagcaattga aaaaaactat tgaaaaaaat
    28741 aactctataa ttgattctaa tctttctaat gctatcaata acatcgaaga agataacctg
    28801 actgtctcac agaagattta tttaacaaac gcaataaaat atcttaaaac aggatctcga
    28861 attgaatttc ctgacgggag taccccaatt gcttatctcg cagctcttct aaatacgctt
    28921 ttacgatatg actcattaat taatgattca gaaaaaaata cagatgagaa aaaagagtat
    28981 caagaatttt atgaaaatga tcttctaaaa gagttcacag ctcttgttac tgagctaaat
    29041 aaacactatt caaaataata ataaactgcc tcctccaacc gcccccacgg cttgctacac
    29101 tatggaggga aaactattat ggcaacattc aagcaatatg aaactaaaaa aggtaaattt
    29161 tggctctatg aagcctatct tggaatgaac aaaatgactg gcaaacctga taaggttcgt
    29221 agacgaggtt acaaaacaaa aaaagaagcc cagcttgcct tgtcacgctt gcaagtggac
    29281 tacgataaaa acggtattaa aaaagcgaac aacgaaacgt ttaaagaagt ttatgacttg
    29341 tggctagaaa catataaaac cactgtccga gaagccacct tcatgaaaac tgaaatcaag
    29401 tttagaaagt ggattcttcc aaaatatgga aatcttcgtg tgaatgaagt cacggtaaaa
    29461 aaagcccaac aaattgtaaa tagctgggct gaaactactg accaatacaa ggtacttcat
    29521 tcaacttcta agcgtatttt tagctacgct atcaatcttg ggattattga gactaatcca
    29581 ctagacaacg tattaatgcc taaaagaacc accactaagc ataaagaaaa ggtcaaagtt
    29641 tacagtaaag aacaactgat caccttgttt gattacctaa acagcaaacc tgaaacttat
    29701 cgaaacgact ttgataaaac gttactacgc tttctctttt atagcggggt taggatcagt
    29761 gaagcccttg cattgaattg gtcagacatt gattttcaag aaaatacggt caccatcaat
    29821 aaaacactaa gccaatccaa aaatggctat aaaatcagtg atcctaaaac tgattgtagt
    29881 gctggggttc ttccactaga tgaaagaaca atttctatat taaaaaaatg gcagattaat
    29941 cagcggaaat atatgctgac acttggtgta actgatccga ctatgatttt ttgtggtatc
    30001 tataaagaac taatcacaca ccacgctatt tatgcaagat tgattactat ttgtgaaaaa
    30061 gctatggttc catttctcgg tgtgcatgta gcaagacaca ctcatgcttc aatgctcttg
    30121 gacgctggag ctactatggt agaagttcaa actagattac ggcatacaaa aattagtcag
    30181 acgatcgatt gttacggtca cttggcaaaa gaaacgaaag aaaagactgt tgaaaaatta
    30241 gtacaacact taaatattga ctagatggca tacaatatgg catacagtaa aaaataaaaa
    30301 aaggagaaac cctatcatat caaggtttct cctagcttta tagtctattc ttcttcttcc
    30361 gccatgaatg tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt ttcgatttgt
    30421 tctaactcgc cttctgttcc atcttcgttt tcgatgtatg cataagcttg taattctaca
    30481 tcttcatctt ctgaaacacc agcaggatat aaaagaacat agttacgtcc aaattcttct
    30541 tgtccgtcaa ctgttagtag gatttcatat aatgtttcat ttccttggtc atccaccaat
    30601 gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat gttctgtcat aagattacct
    30661 cttttctttt attattaaaa taattgctta cctgagccgt ctaaataatt ttgtaagatc
    30721 atcacagctg ctaatttatc gatgactttt tttcgtttgg ctcttgaagt atttgcctgc
    30781 tctactagca tacgttctgc ctgaacagtg gttaatcgtt catcttgata atcaactggc
    30841 aaggagaata gttctttgat cttttctccg tatgacatgg aagcttctgc ccttggtccg
    30901 atggaattgt tcatattttt aggtaatcca actacaaacc ggtctacttc atattgtgct
    30961 actaattcct tcaaccgatc aaagccaaac tcttcttctg cttcattgat ccgaatgatc
    31021 tctactcctt gagctgtcca gccaaagggg tcactgatag caacacctac tgtcttagaa
    31081 ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg tagtccttga
    31141 caattacttc catgatctca tcgcgttcgt gacggcgaat caaatttcga gcatcacgat
    31201 aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga ttgattggat
    31261 tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc acttcttttt
    31321 tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg attctacacc
    31381 tctttcccgc aaggtaattc cagcttacct tctacaacct tattttactc gaagaaaggg
    31441 aaaactacaa acaaatattc caaacttccg aataaattaa gaaacactat ttgtatgttt
    31501 ttttcctttt ttctgtagct ttatcaaaag tactcttttc ataaagtata cgctatattt
    31561 ctttgaatgt atgtttttac acaatttttt cataatattc ataaaaaatt cgaatagctg
    31621 ctactaattt tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt agccatcttg
    31681 aacattgtag ataaagacat tgaattcaat ttttttgtct tcaatgcttt ctgcttccaa
    31741 ttgcactccc ctggctacca gttcattccc ctcaaaatga ggagtgacac ggtagcgtac
    31801 atgatgattt gtttctttga tatatgccgc aatatcattt tcgtgagcaa gcatgtatgg
    31861 agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt gaccagtcag
    31921 ctggaaacca attaaatggc tgcgattata taaccattgc ccgtcactta atttcttttg
    31981 tttccaacct gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga acaaatcttt
    32041 tccaagcata gcattggctt gtcctactct gttcaattta tcaatatttg aatagctttc
    32101 ccatgttcct ttggaaaggc taagatcctc ttttgtaaat tccggctgat tcccattgat
    32161 ttctatttct tgatgttttc catcatattc tggcacatcc gctacttgag aagcttgtgg
    32221 tgtggaatca aaaatagttg taatctgctg aaagatatct gaatcttttt gtgtggtgga
    32281 aacaccgaat gcccctgcaa tcacagcagc gacgatcccc acgatcgcct tggttgtttt
    32341 tttgctcatt tctttactcc tttactaaca gctccctaaa tcttaacata tgttccccta
    32401 taaataaata ctagaaagaa atagaaacag caaaaaataa aacctgtttt aagatggctt
    32461 aaaaatacaa aaaagagacg gattcctcgt ctctaatcag tcaccactct atctgtaagg
    32521 tatttattat tattcaaaaa ttttcttcat actcaagaca atacactgat ttcacaaccg
    32581 cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca agttaaaaac
    32641 ataagaagtt gtttttgctt ggctgttctt attacttgtt gcagagcagc tttttcacaa
    32701 cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt
    32761 taccactaaa ctacacccgc aatacaaaaa cagtatacgc ctcttttatt ccttcctcaa
    32821 gtttttttat tctttacgct tctggtatgt tttgtagtaa aataaaacta atcaatcaat
    32881 tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact
    32941 cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag
    33001 tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct
    33061 ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat
    33121 taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct
    33181 aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc
    33241 tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg
    33301 cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat acgcagctta
    33361 tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat cttctttatt
    33421 gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc aggatctaat
    33481 cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg ctatccgtga
    33541 tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga tgatgactgc
    33601 tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg aaaggaacac
    33661 aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg cggcatttgc
    33721 cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg
    33781 ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg gttcattgct
    33841 tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga aaatgcccgt
    33901 aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag cttatcaagc
    33961 ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg tccaagcaat
    34021 tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta accataacat
    34081 ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa
    34141 ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg agcgatgaca
    34201 aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta attatgagta
    34261 ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg
    34321 agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc
    34381 acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat
    34441 agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa
    34501 aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc
    34561 atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc
    34621 cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag
    34681 ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt
    34741 ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac
    34801 ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct
    34861 tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat
    34921 gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt
    34981 tcgcctccta gggcaaaagc aagaataaaa atgattggag ttaacaagga agaagaaaga
    35041 acttgcataa tcaaattagg ttcttttact aattgagcat tgtaactaaa taaaagctga
    35101 tgcacatttt ggttcttttt atgggttctt tttattttga actcactgtt tgaatcagtc
    35161 agttgctctg taagctttgg tactatcagc tgttttacag caaggaaaaa tcccagagcc
    35221 gtgatagcta gtattcccca acccaataaa ccttgttgag aaagtggctg actggcagca
    35281 tcatagaatg gcaataatat cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc
    35341 cccgtttgat gattcatcca catgattcca gcaaatgcga tcagcatcga gctgccaagc
    35401 aagatactag aaactgtttt tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa
    35461 acaatcaaac tagccagaca aaaaagaata atcaaaaata gagaaaacaa aaggattgag
    35521 ataatgatag taagcaaaat ccctagatca gctcgccatc cggttaaaat aaacatgact
    35581 agaagcggaa aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa
    35641 acctgttctt gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag
    35701 atattataaa tgactgatat actttgagtt aaacctaaca taccaaaaag tgccacgtaa
    35761 taagtaaaga aacctggcaa tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc
    35821 agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa gatagatagt taatcgcttt
    35881 cccttttttc ctttctctct tgctttattc gtgagctgtg gattaacata taaaagatta
    35941 actcgaatta attctaataa ttgtcgctta tacatacggt ccatctcctg tcgcttgcct
    36001 tccagccatt ttcaaataaa tcttttctag cgtttcatca ggtgagccat tcaataattc
    36061 actaactgcc ccctgataaa tcaattcacc tttcttcaaa atagccagtt tatcgcataa
    36121 ctgttgtgcg gtatccaata catgtgtaga aaaaatgact gtttttcctt ttgcggcatg
    36181 agccttcatc atttctttca aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc
    36241 atccaatacc caaatatctg gatctggcag caacgtgccg atcaaaatcg ttttttgacg
    36301 cattccatgt gaaaaacttg cgattgtttc attttgatga ctatacatat caaaaagcgc
    36361 tgtcaattct gctaaacgct tttctttttt ttgtgtgtct aattcatagg cagctgaaat
    36421 caagtcccaa tattctccag ccgtcaattg taaaaaaata tctggggaat caggcacata
    36481 accgattttt tgtttgatac tcaatcgatt ttccgtcaat ttcattccat ctactaaaat
    36541 cgttcccgat gatggttcaa taatactaac taaactttta atcgtagttg attttccggc
    36601 accattatgt cctaaaaatc caaaaatttc cccttgtctt atcgagagag acaagtcttt
    36661 taatgcttgt tttttcccgt aacttttact tacattgatg aattcaatca tttctgtact
    36721 ccttttgttt gttattactt tttattgtac ccttcgttat tggtggagta aatgtcaaaa
    36781 agagtgcata acaaaaaagg atagaaacga ctaggctgct acttgggttg ttctcttcta
    36841 caggaaaaca ctcttctaac aggtaagata tgagtctttg ttttctttaa aatgattcat
    36901 tttctatctt ttcttgcata aaattcttta gtttatcaat acttctcttt cccatctcta
    36961 gttgttctcc atttgcaaat gttactattt cgtttactct gctaatagat gtaatgtttt
    37021 cagtattaat gatataagct tttaaatctt tacataaata ctcatattcg agtgattttt
    37081 tatatttttt aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt
    37141 tatttttata acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat
    37201 cgctattacc ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt
    37261 tttctttttc tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt
    37321 cttttatcaa ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg
    37381 gatgagacgt tagataaaca attttacact tttcattctt ttctctaagc atttcgccta
    37441 gttcgatacc gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat
    37501 agatttcatt atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct
    37561 caaatttcca aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt
    37621 caaaaaccaa actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc
    37681 gatattttac tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt
    37741 gtaattctct tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat
    37801 gctgcttcta aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc
    37861 gacgtccttg cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa
    37921 cgatacacat actgattttt cggagcagag actttctcag cgtttgtaac aactacagat
    37981 gggatttcac gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac
    38041 cattcatcat ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct
    38101 gaatctgcca cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg
    38161 ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg
    38221 taattataaa ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc
    38281 ttatccccat acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa
    38341 taaatactgg tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct
    38401 ttacttactg ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt
    38461 ggaaacaacg tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga
    38521 taaggttttg acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg
    38581 tttttgaccc acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga
    38641 atagctaagt agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag
    38701 tacatcatca attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta
    38761 aataaataat gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa
    38821 gcgtcctgta aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa
    38881 gaccaaaaag gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa
    38941 cgcatctgct tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc
    39001 taaaatcttt gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc
    39061 agctgcgatt aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga
    39121 atgtgtcaac acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact
    39181 ttgttcttct tcatggacat gatgatggtg attttctgct actttagacg tgattttttc
    39241 tttcaataca gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa
    39301 aaagctgcct atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt
    39361 ggaaaaaatc acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg
    39421 aactggtacg ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga
    39481 aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag
    39541 ccatctcttc acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc
    39601 taataatacg aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa
    39661 aaccgaattt ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc
    39721 tttgcaaata tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact
    39781 cttatttttt accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc
    39841 tcatacatat cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga
    39901 ctgaagttca agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta
    39961 attgcagtgt ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata
    40021 ttccggcact tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc
    40081 tgactgcgcc agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt
    40141 tcatcgactt taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc
    40201 tttcttatga agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg
    40261 aacaagtgaa agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt
    40321 ttcggaatgc gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt
    40381 caaatcaaca ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt
    40441 caagattcct ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc
    40501 gggtatcgtt aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc
    40561 cattggataa acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa
    40621 agcacaactg atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc
    40681 caagttccct acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc
    40741 aaaaaagtgc ataagatctt ctacatcata accataccca cgttttccta attcgccaaa
    40801 ataagaaaaa tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt
    40861 tgaagacact tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg
    40921 cagtctttta gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat
    40981 tgtctaatta catctttgat ttctccatca tagcagtatt tataggaaaa aatcaattcc
    41041 attgctcaga tattgtgaaa ttttattctt tattcattta tttatcattt ctgcaaatct
    41101 cgcagtataa tggaatttat ggtagactac taattgaaaa atagtttttt atttgaggag
    41161 aattcacaaa gtagaaaaga ggattccatg atattattac aagccaatca gattgctcgc
    41221 cattttggtg cagaagtctt atttgaaaac atacatttag aaatcagtac aggcgctcgt
    41281 gtcgctttag ttggcagaaa tggcgctgga aaatcgactt tattaaagat cattgctgga
    41341 attgaagcac ctgatgaagg aaagatcgct aaaaataaaa cagcgactct cggatacctc
    41401 gctcaggaca caggacttga ttccaatgaa acagtttgga acgagatgct gaaagctttt
    41461 gaacaagtta gacaaatgga agagcggatg cgcgagctag agattttcat tagcgaaggt
    41521 acgccagaca cacctgctta tagttcttat ttaaaagaat acgaccggct gcaacacgat
    41581 ttttcagatc gaaacggcta tggttatgaa aatgaaattc gttccgtatt gcatgggttt
    41641 aaattcgatt cttcatttta tgatcagaaa atcgatactt tatcaggcgg acagcgcaca
    41701 cgtttagctt tagctcgcat gcttttgcaa caccctgaca ttttgattct agatgaacca
    41761 acgaaccatt tagatatcga gacactttcc tggcttgaga attatctgca aagctatact
    41821 ggtgcattat tgattgtttc ccatgaccgt tattttttag ataaagtagt caatgaagtc
    41881 tatgaattaa gcagaagaaa aatgcgccat tacaaaggta actattcgaa atatctagat
    41941 ttgaaagcag aacagttagc aagtgaatgg aaggcttatg aaaagcagca aacagaaatc
    42001 cataaattag aagacttcgt tgctcggaac cttgttcgcg cctctaccac aaaacgagcg
    42061 caaagccgca gaaagacatt agaaaaaatg gaccggctgg atcgtcctca aggggatgaa
    42121 aaacaagcaa actttctatt cagtatagaa aagccttctg gaaatgtagt cttgcaaatc
    42181 gaagatgccg ctatcggata tgataatgtc accttatctg aaccaatcaa tctagatatc
    42241 cgtcgtcaag aagcgattgc tctagtaggt ccgaatggta tcgggaaatc gacgttgctg
    42301 aaatcaatca ttcaccagat cccttttatc cgtggaaaag aaacgctagg gacaaatgtc
    42361 accatcggct attacgatca aggtcaagct gatctgcata caaacaagac gatcctagca
    42421 gaattgtggg atgagcatcc taccactcca gaaaaagaga tccgaagtgc tttaggtgct
    42481 ttcttatttt ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa
    42541 gcacgggttg ctttagccaa gttagcgatg aacaaagaaa attttttgat tttggatgaa
    42601 ccaacaaacc atttagatat tgacaacaaa gaagtattag aaaatgcatt gatcgattat
    42661 gacggcacac tgctttttgt ttcccatgac cgatatttca tcaatcggat cgcgacaaaa
    42721 gtcgtcgaac tctctgaaaa tggcagcaaa gtctatctag gagactatga ttattattta
    42781 gagaaaaagc aggaagaaga agaaatcgcc gaattgttgg cacaagatga gccagaatct
    42841 gctcctgctc ccacgaacaa atctgattat taccaaagca aagaacagca aaagcttatc
    42901 cgtagccttc aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg
    42961 atcgatcagt tggaacaaga aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc
    43021 aatgagctga accaagattt agaagctgct agacaagatc aagaaacaaa attgacggaa
    43081 tgggaagaac taagcttgga attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga
    43141 aaccattcat gctgttttgg tttttctttt tttattttat caaacaaaaa ttccatattt
    43201 acctttttca ccaataagaa gaccaattac tagtcattct cactaaagct tctaaataat
    43261 agtaatcacc ccaaatacaa ggtgtattta ccccattatt tttgggctta ttataaacag
    43321 actgtgttaa aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca
    43381 taattttcca aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt
    43441 ctattaattc taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc
    43501 tgctagaatc atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca
    43561 taaaataatt ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat
    43621 aacttaatgc aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac
    43681 tataaccttg atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg
    43741 tatgataagt tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct
    43801 gagcagcttt tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa
    43861 gattcatgca acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc
    43921 aagcttgtat aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa
    43981 ttgaagcttc cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag
    44041 aatacaaaaa accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa
    44101 aatctatatc tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata
    44161 acaaccaatt aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat
    44221 aacgtccttt tataggacac tcgcttggga attgatactg actgttactg atacgtttga
    44281 ttaacctctc cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta
    44341 attctttata acattcattt atccattttt ctggatgtat tatctcttca aacatcactt
    44401 tatcaagcat ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt
    44461 ttggatagtt agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc
    44521 taaactataa tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt
    44581 tccaaatgta ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt
    44641 gggcaaaccc ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat
    44701 acctgaaaag ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc
    44761 ttctttctca ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc
    44821 taattctact ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat
    44881 ttttgcctgt aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc
    44941 tcgtacatta tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact
    45001 ggtcgtgata tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc
    45061 tttcaattga ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa
    45121 atatttttca ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa
    45181 tgtatgtcca ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc
    45241 acgaataggc ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa
    45301 caataaaaaa atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa
    45361 tgtcaattga gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt
    45421 ttgcatccac catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg
    45481 caaaagatcg gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat
    45541 gcttcgacca aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga
    45601 aaaatcaata gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct
    45661 ataaatcaaa ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga
    45721 actgtctcga taccagccat tttgaagata acaattttct atattatcaa atgcttcttt
    45781 cagtgctgat tctttaactg gataaccaaa tttttggata attgttccga caattatctt
    45841 aaaaaattgc caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat
    45901 aatctggctt ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa
    45961 cgctaataac attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca
    46021 ataatcggga tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca
    46081 tcgttcatct aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc
    46141 cattcctgca attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc
    46201 acttgctcga taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc
    46261 ctgtctggtc cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac
    46321 caaatagcta attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa
    46381 tagatcagtg gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg
    46441 atcccatctt catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg
    46501 atagcagctt catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga
    46561 agaccgcatg cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa
    46621 tcaatgagag gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt
    46681 tctgctatag ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat
    46741 aaagcccatg catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt
    46801 aaccgaatat atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct
    46861 ttcctcacta gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc
    46921 ggatcaccag tttccctcga agcccaatac agtaatggta aattcatcat actatcaata
    46981 atcatccaac caggtttatg gtcattccat gctactaaat agttccctga aatattatag
    47041 cgtccagcaa gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac
    47101 tgttgataat gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct
    47161 gaaaaatggt acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac
    47221 ttttctcgtt ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc
    47281 caccagtcga ttcctctgtc ctttattacg tctgtatatt ttccgttgta agaaatatat
    47341 ggaattttat tccccattct gtctaattcc gcatctatct tttcagaagc tacattcaaa
    47401 atttttcttg accattcctt cgtctgttca ttcatggata tcccgccatt cttttaattt
    47461 ttatgaatgt ggagttgctc ctagaacgaa ctagatggtt aacattcgtt atttttttta
    47521 cttatttgca gctccaatgt cattctcctt aaatttagac tgcgcccaca cataaatcaa
    47581 gaccattggg atcactgaag ttgctaaaat tagcataatt gcgccataat taattggctc
    47641 acctgtacta gaaaccgtca tatttttgat aattaaggga accgtatatt tatctttatc
    47701 tgaaagcatg actaatggaa atagatattg attccaactt cccataaaga taagtaaacc
    47761 actagtaata atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc
    47821 attggcacca tcgattctgg cagcctccat cagtgctgta ggaaattgac tgctatattg
    47881 tcgcatcata aacactgaag ttgtactggc aatcgaaggg atgatcacac ctgcataagt
    47941 attcgataat cctaaacgac tcataatgta aaataaagga atcaatgtgg taaaaaaagg
    48001 caccatccta gatagcataa ctgtattgaa caacagttta ttccccataa aacgatactt
    48061 tgctaatgca taacccgcca ttgtagaact ggctgtagct aaaactgtac tgattacagc
    48121 aataacaatc gaattccaaa taatcaaggg ataattatat ctcgccatga tggcttgcca
    48181 gttttttaat agcatatccc caaacttgaa attgattccg ccacttaaaa tttcttgatt
    48241 actttgcgtt gaagctacaa tcatatataa atatggcatc aaaaatatta aggcaccaaa
    48301 cacaataatg acgtacgtaa aaattctgct ttgtatcttt tgtttagtca tttttatcac
    48361 ctccgaattt aaattgaata gccgataata taacagatac aaacaccatc gtccaggcaa
    48421 tagcagaggc ttttccaaaa tcaaccattt caaaacttgt tttatataaa ttgactccta
    48481 cgaccaaagc tgaattttct ggtccattgg aagtcccaaa gaatatattt gggatattga
    48541 aattacccaa gccattgata gtggctaaaa ctgtggccat taaaatgatt ggctttagca
    48601 tggggatcgt taataaaaag aattgccgaa atttcgatgc tccatctatt tcagcacttt
    48661 cgtatatctc tttagaaatc ccttgaagac cagctaaaaa taaaatagtg ttatatccta
    48721 cactaagcca aatcatcgtg atgatcattg atattcgtat ccaaaaagga tttgttaacc
    48781 actgtacacc gttaattcct agcgcattca aaaatccatt gaataaccca gtcggattga
    48841 aaaaagtcat gaatacggaa gctacagcca ccgttgatgt aatggttgga atataataga
    48901 cagttctaaa aaatcctttt ccttttaact gtgctgaatt caataaaaca gcaaagtaaa
    48961 tagctaatag catcgttatt ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag
    49021 atttaataaa caatggatct cggaagatag ctagataatt gtcaaatcca acaaattcaa
    49081 atgagtagcc aattttcata aaactaaaag caaacgagat aatgatggga atcaaaatcg
    49141 tcacaataaa gaaaataaca aaaggtgtga ccaatccata agcacttttt ttatgagacg
    49201 tatctgttgc ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa gattgcatca
    49261 tcgttaagtc ctcctaatat tttttaatca ccagttaatt ttacgccgta cttatgagca
    49321 aaattttctg cttcttttag atatgaattt tcaaaatcac ctgtttgcca atagttatat
    49381 gcagcggtag tcaaataaga cgtagcatct gaataagtgg ctggtaaaac agtatttgat
    49441 gtattttcat tgattttatt caatagttca tagtattttt gtcctcctta gtagtcaaac
    49501 tttttctcag ctgcttctgt ttgataggcc aatgagttag atgtagccac gcccagctcc
    49561 agtgcgttgg ctaagcattc ttcatctgtc agcataaatg taagaaattg ttgtgccact
    49621 tgtggatttt tggcatcttt agggacataa aaacttgatc ctcctgatac tgcacctttt
    49681 cctggatttt cttttgaaaa aggaatcagt ggagccatgc gccatttgtc agatgcatca
    49741 ggaaaattca aagacatatt cgtagcatac cagccgccag caacaaacat tgcagattct
    49801 tgaaatgctt tctcagcatc ttgtgcaccg tagaaatgta caatatctga gtcgatcatt
    49861 tctttgatta actttgcagc atgtttcgct tctttagtat tcagacggat atttccttct
    49921 tcatcaagca aagattgatt ctgctgggct agcatgttga taaacaattc ttggtcccca
    49981 gattctggca aggcaattcc aaaactacca gttttttctt ttaatgcttt tgccacctgg
    50041 atatattctt cccaagaact gatttcttca aaatctacac ctatttcagc aaattttttt
    50101 ggttgataat aacaaagtgc tggggtaaaa tcactagccc aaggaataat cgtttcatta
    50161 ctcgtttgtt gacgcaaaat attcaatttt tgatcataaa actgatcgcc atatttgtca
    50221 taaaatccat attcatcagc actataaaaa gcatcttgaa atttttccag cacacctgct
    50281 gctgaagtgt ctgccatgga gataatatcc ggcatatctt cgtgtgttac caattttggg
    50341 gtgatcattt gtactgttgc tgttcccgaa gctacattca caatttttaa gtcaatattt
    50401 gctccagttt tgcggttata gatttttgca gcattttcaa catagtttgt cagtttgccg
    50461 tttgcgacat atacttctaa tgtagtggct ttttttatca ttggatctgc gatgtttgct
    50521 gttacttctt cgttagtttt tccacatgca cttaagctga ataatagggt aatgtttagc
    50581 aaagctatca ccaatttgta tctttttttc atgtctatcc cttctttctt ttcaagttct
    50641 tatataaaac gctttcactt atgtatatta acggaattag taacacaaat atttatataa
    50701 aaaaatattt tacttgtcgt tttggctttt catttttgtt ataatagtca ttaattataa
    50761 atagaaagct ttttatataa aaagagaaag gaaagtggtt cttttgttaa caaacaacca
    50821 atcctccaat cttgtcaaaa aaacactaac ctattccata gatacccatc ttcatttatc
    50881 tgctgctccc tattttcgac tactaataaa tgaaagcgct aatttatatg ctgaatttgc
    50941 acataaatct atagaactag ataatccttc ccttttattc attgataaac agcaagaagt
    51001 ccaaatcttt tctactcaaa aggaaacaca aacttttatc attatccagt tttattttga
    51061 tgagcaatta ttagcacata ttacgggaca atctttttct atagatcaag aaataacatc
    51121 tatcattact tttctgaaaa atgaacagtt caaaattgat tatctaaaac atagtaagcc
    51181 agctatttcc tatgatcagg aaatcaattc ttttttcata ctatgccaac agatgagcat
    51241 tcatttgctt tccttattag ttacttattg tctgcaaaaa attcaattgt cttccttacc
    51301 aaggcattat caacaggaac ttgctttatt gacaatacct tcaaatgaga aagtcattcc
    51361 taaaaaaaag catattcttt attcagatga attagttgat ccaatcatac gatatttaga
    51421 agaacatctt tcagaaaatt ttttgcttga acaattagcc caaatttttt ttatcagtcc
    51481 agccagctta aaaaaacatt ttagaaaagt gaatggaaaa agtgttatta gctattttaa
    51541 agaactaaaa atggaaaaag caaaacgtct cgtgctagaa aatgaattaa cctatacaga
    51601 aattgcagat atattaggat ttcattcgat ccatcatttt tcttcagctt ttaaaaaata
    51661 tacagggtta tccccttcaa agtattatct atccttaaaa tagaaaaaaa tttttaggtt
    51721 ttatccacaa attatttctc tatattttct gtctattcaa aataaacaat aattttccgg
    51781 cacaattatt tgctgttttt taccataaca tagtacgatc aacgtagaaa aggagatgtt
    51841 tttatgaaag ttgttgtcat tggcggtgta gctggcggtc cttcatttgc cactcgtttc
    51901 cgtcgattga atgaagcgca cgaaatcatt atctatgaac gcggagaaaa tatttcttat
    51961 gcaagttgtg ctttgcctta ttatttagga ggtgtgatca ctgaccgtga ctcgctgatc
    52021 gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg atgtctttac taaacacgaa
    52081 gtaacagcaa tcgatccttc tactaagcga ttaacagtta aagacctatc cacaaatgaa
    52141 gaaataaaaa cagattacga taagttgatc atctcttctg gtgctagacc agattatccg
    52201 gatattcccg gagtttttga agcagaaaac ggctttgtcc tccgtagtgt gacggatgcg
    52261 gatcgaatca aatcgttcct tgaagaaaaa aatccacaac atgtcgtcat tcttggtgca
    52321 ggtgttatgg gtctggaatt agctgagaat ttcaagcatc gcggcttaga cgtgacttta
    52381 atcgatcaat tgccacaagt cgctttccct tatgatccag aaattgctaa tttagtttat
    52441 gacaaattgc tgaaagaagg attagccgtt catttagaaa caagagttac tgagatccgt
    52501 gataaaggtc gagaaatagt attatcagat ggctccgtcc tttctgctga tatgctaatt
    52561 tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag caaccggcat acaattatca
    52621 gatacaggtc agatcattgt tgatgaccag ttacaaacca atctgccgga catctatgcg
    52681 attggcgata ttatcgaaac gactagtgta gtgactggtc agccgatcca aagtatgctt
    52741 tccagtgcgg ccaatcgtca aggacatatg ctggcagata ttttaaatgg tacgcctatg
    52801 cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct ttgatcatac agcaagttat
    52861 gcgggaatga cagaacatga attaaaagca tcaggcataa caaattataa aactgttttt
    52921 atcactccct ttgaccatgc ctatttctat ccagaagcta caagattaaa tctaaagctg
    52981 atttttgatg cagatagcgg tcgtatttta ggtggacaag catttggaga aaaaggtgtc
    53041 gataaacgga tgggagaact ttctgtagca atcaccggaa acttgacagt ctttgatttg
    53101 cctgatttgg agttgcctta ctctccaccg tattctacta cccgtgaccc gttgaatata
    53161 gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag aaacgatcaa agcaagtgat
    53221 atacccgaaa acgatttgaa agaagcgttc tttttagata tacgtgaacc taataaagca
    53281 ccttctggca gtatctcggc aacaaaaaat attccaatga atgagttacg agatcgaatc
    53341 aatgaaatcc caaaagataa aaaagtttac attactttca gaagaggatt gaatacttat
    53401 acttctgccc gaatcttggc aggtttgggt atcaaagcga ttttgattga agaataaaaa
    53461 aacgaccgcg gtgggtagcc gcggtctagg agttattttt acttgaggag taaaaatgaa
    53521 aaagtatata gggttgttgg ttggtatgta tatagaatag aagataaatg tgaagaaatt
    53581 gtgcattatt tcttcacatt tattaggtta tagtttaaga aagtttaaac attgcgcaat
    53641 ttaattattt tagctgtttc ttttcataaa aaaacatgtt gtgactctga gacaaatcat
    53701 agtccatagt gatgtctcta gagtaactac gcctaatatt acgaggcatc atattaatct
    53761 atcagcacta gtacctaaac tagcatccct ttcgaatatt ttttcttata taataaagtt
    53821 tgctttcact cctctaatct caccaaatcg actggcttgc gttgtgtgat actaggaact
    53881 gattggttta gaacgacttt tctattgtaa tcatattttt cgccatcctc gttgtcttct
    53941 aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa tttaattttt ttgttcctat
    54001 ttatttaa
//
LOCUS       DAZKCN010000009        47443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000009 DAZKCN010000000
VERSION     DAZKCN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.009"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..211
                     /locus_tag="ACG8K3_000463"
     CDS             23..211
                     /locus_tag="ACG8K3_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP9144369.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(269..622)
                     /locus_tag="ACG8K3_000464"
     CDS             complement(269..622)
                     /locus_tag="ACG8K3_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144370.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1153..1575
                     /locus_tag="ACG8K3_000465"
     CDS             1153..1575
                     /locus_tag="ACG8K3_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9144371.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1572..1802
                     /locus_tag="ACG8K3_000466"
     CDS             1572..1802
                     /locus_tag="ACG8K3_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144372.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2300..2500
                     /locus_tag="ACG8K3_000467"
     CDS             2300..2500
                     /locus_tag="ACG8K3_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP9144373.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2527..3720
                     /locus_tag="ACG8K3_000468"
     CDS             2527..3720
                     /locus_tag="ACG8K3_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9144374.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3788..5353)
                     /gene="guaA"
                     /locus_tag="ACG8K3_000469"
     CDS             complement(3788..5353)
                     /gene="guaA"
                     /locus_tag="ACG8K3_000469"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9144375.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5412..6335)
                     /gene="coaA"
                     /locus_tag="ACG8K3_000470"
     CDS             complement(5412..6335)
                     /gene="coaA"
                     /locus_tag="ACG8K3_000470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9144376.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6531..7136
                     /locus_tag="ACG8K3_000471"
     CDS             6531..7136
                     /locus_tag="ACG8K3_000471"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9144377.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7444..8460
                     /locus_tag="ACG8K3_000472"
     CDS             7444..8460
                     /locus_tag="ACG8K3_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9144378.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8601..9902
                     /locus_tag="ACG8K3_000473"
     CDS             8601..9902
                     /locus_tag="ACG8K3_000473"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP9144379.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9948..10610
                     /gene="deoC"
                     /locus_tag="ACG8K3_000474"
     CDS             9948..10610
                     /gene="deoC"
                     /locus_tag="ACG8K3_000474"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9144380.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10631..11029
                     /locus_tag="ACG8K3_000475"
     CDS             10631..11029
                     /locus_tag="ACG8K3_000475"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9144381.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11095..12177
                     /locus_tag="ACG8K3_000476"
     CDS             11095..12177
                     /locus_tag="ACG8K3_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9144382.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12389..13951
                     /locus_tag="ACG8K3_000477"
     CDS             12389..13951
                     /locus_tag="ACG8K3_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144383.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13944..15077
                     /locus_tag="ACG8K3_000478"
     CDS             13944..15077
                     /locus_tag="ACG8K3_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144384.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15077..16033
                     /locus_tag="ACG8K3_000479"
     CDS             15077..16033
                     /locus_tag="ACG8K3_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144385.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16132..17298
                     /gene="deoB"
                     /locus_tag="ACG8K3_000480"
     CDS             16132..17298
                     /gene="deoB"
                     /locus_tag="ACG8K3_000480"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9144386.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17322..18140
                     /locus_tag="ACG8K3_000481"
     CDS             17322..18140
                     /locus_tag="ACG8K3_000481"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9144387.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18157..18864
                     /gene="deoD"
                     /locus_tag="ACG8K3_000482"
     CDS             18157..18864
                     /gene="deoD"
                     /locus_tag="ACG8K3_000482"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9144388.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19162..19848)
                     /gene="gpmA"
                     /locus_tag="ACG8K3_000483"
     CDS             complement(19162..19848)
                     /gene="gpmA"
                     /locus_tag="ACG8K3_000483"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9144389.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20020..20601)
                     /locus_tag="ACG8K3_000484"
     CDS             complement(20020..20601)
                     /locus_tag="ACG8K3_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9144390.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20734..21417)
                     /gene="rpiA"
                     /locus_tag="ACG8K3_000485"
     CDS             complement(20734..21417)
                     /gene="rpiA"
                     /locus_tag="ACG8K3_000485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9144391.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21736..22149
                     /gene="rpsL"
                     /locus_tag="ACG8K3_000486"
     CDS             21736..22149
                     /gene="rpsL"
                     /locus_tag="ACG8K3_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9144392.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22233..22703
                     /gene="rpsG"
                     /locus_tag="ACG8K3_000487"
     CDS             22233..22703
                     /gene="rpsG"
                     /locus_tag="ACG8K3_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9144393.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22797..24884
                     /gene="fusA"
                     /locus_tag="ACG8K3_000488"
     CDS             22797..24884
                     /gene="fusA"
                     /locus_tag="ACG8K3_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9144394.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25056..26243
                     /gene="tuf"
                     /locus_tag="ACG8K3_000489"
     CDS             25056..26243
                     /gene="tuf"
                     /locus_tag="ACG8K3_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9144395.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            26650..26958
                     /gene="rpsJ"
                     /locus_tag="ACG8K3_000490"
     CDS             26650..26958
                     /gene="rpsJ"
                     /locus_tag="ACG8K3_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9144396.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            26989..27618
                     /gene="rplC"
                     /locus_tag="ACG8K3_000491"
     CDS             26989..27618
                     /gene="rplC"
                     /locus_tag="ACG8K3_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9144397.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            27646..28269
                     /gene="rplD"
                     /locus_tag="ACG8K3_000492"
     CDS             27646..28269
                     /gene="rplD"
                     /locus_tag="ACG8K3_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9144398.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28269..28559
                     /gene="rplW"
                     /locus_tag="ACG8K3_000493"
     CDS             28269..28559
                     /gene="rplW"
                     /locus_tag="ACG8K3_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9144399.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            28598..29431
                     /gene="rplB"
                     /locus_tag="ACG8K3_000494"
     CDS             28598..29431
                     /gene="rplB"
                     /locus_tag="ACG8K3_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9144400.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29474..29752
                     /gene="rpsS"
                     /locus_tag="ACG8K3_000495"
     CDS             29474..29752
                     /gene="rpsS"
                     /locus_tag="ACG8K3_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9144401.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            29775..30122
                     /gene="rplV"
                     /locus_tag="ACG8K3_000496"
     CDS             29775..30122
                     /gene="rplV"
                     /locus_tag="ACG8K3_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9144402.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30136..30792
                     /gene="rpsC"
                     /locus_tag="ACG8K3_000497"
     CDS             30136..30792
                     /gene="rpsC"
                     /locus_tag="ACG8K3_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9144403.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            30795..31229
                     /gene="rplP"
                     /locus_tag="ACG8K3_000498"
     CDS             30795..31229
                     /gene="rplP"
                     /locus_tag="ACG8K3_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9144404.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31219..31407
                     /gene="rpmC"
                     /locus_tag="ACG8K3_000499"
     CDS             31219..31407
                     /gene="rpmC"
                     /locus_tag="ACG8K3_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9144405.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31432..31698
                     /gene="rpsQ"
                     /locus_tag="ACG8K3_000500"
     CDS             31432..31698
                     /gene="rpsQ"
                     /locus_tag="ACG8K3_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9144406.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            31756..32124
                     /gene="rplN"
                     /locus_tag="ACG8K3_000501"
     CDS             31756..32124
                     /gene="rplN"
                     /locus_tag="ACG8K3_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9144407.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32161..32469
                     /gene="rplX"
                     /locus_tag="ACG8K3_000502"
     CDS             32161..32469
                     /gene="rplX"
                     /locus_tag="ACG8K3_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9144408.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32497..33036
                     /gene="rplE"
                     /locus_tag="ACG8K3_000503"
     CDS             32497..33036
                     /gene="rplE"
                     /locus_tag="ACG8K3_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9144409.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33055..33240
                     /locus_tag="ACG8K3_000504"
     CDS             33055..33240
                     /locus_tag="ACG8K3_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP9144410.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33276..33674
                     /gene="rpsH"
                     /locus_tag="ACG8K3_000505"
     CDS             33276..33674
                     /gene="rpsH"
                     /locus_tag="ACG8K3_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9144411.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            33706..34242
                     /gene="rplF"
                     /locus_tag="ACG8K3_000506"
     CDS             33706..34242
                     /gene="rplF"
                     /locus_tag="ACG8K3_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9144412.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34403..34759
                     /gene="rplR"
                     /locus_tag="ACG8K3_000507"
     CDS             34403..34759
                     /gene="rplR"
                     /locus_tag="ACG8K3_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9144413.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            34780..35280
                     /gene="rpsE"
                     /locus_tag="ACG8K3_000508"
     CDS             34780..35280
                     /gene="rpsE"
                     /locus_tag="ACG8K3_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9144414.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35295..35474
                     /gene="rpmD"
                     /locus_tag="ACG8K3_000509"
     CDS             35295..35474
                     /gene="rpmD"
                     /locus_tag="ACG8K3_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9144415.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            35518..35958
                     /gene="rplO"
                     /locus_tag="ACG8K3_000510"
     CDS             35518..35958
                     /gene="rplO"
                     /locus_tag="ACG8K3_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9144416.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            35958..37253
                     /gene="secY"
                     /locus_tag="ACG8K3_000511"
     CDS             35958..37253
                     /gene="secY"
                     /locus_tag="ACG8K3_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9144417.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37315..37962
                     /locus_tag="ACG8K3_000512"
     CDS             37315..37962
                     /locus_tag="ACG8K3_000512"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9144418.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38153..38371
                     /gene="infA"
                     /locus_tag="ACG8K3_000513"
     CDS             38153..38371
                     /gene="infA"
                     /locus_tag="ACG8K3_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9144419.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38404..38520
                     /gene="rpmJ"
                     /locus_tag="ACG8K3_000514"
     CDS             38404..38520
                     /gene="rpmJ"
                     /locus_tag="ACG8K3_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9144420.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            38538..38903
                     /gene="rpsM"
                     /locus_tag="ACG8K3_000515"
     CDS             38538..38903
                     /gene="rpsM"
                     /locus_tag="ACG8K3_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9144421.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            38931..39320
                     /gene="rpsK"
                     /locus_tag="ACG8K3_000516"
     CDS             38931..39320
                     /gene="rpsK"
                     /locus_tag="ACG8K3_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9144422.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39400..40338
                     /locus_tag="ACG8K3_000517"
     CDS             39400..40338
                     /locus_tag="ACG8K3_000517"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9144423.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40366..40746
                     /gene="rplQ"
                     /locus_tag="ACG8K3_000518"
     CDS             40366..40746
                     /gene="rplQ"
                     /locus_tag="ACG8K3_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9144424.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41018..41857
                     /locus_tag="ACG8K3_000519"
     CDS             41018..41857
                     /locus_tag="ACG8K3_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9144425.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            41833..42702
                     /locus_tag="ACG8K3_000520"
     CDS             41833..42702
                     /locus_tag="ACG8K3_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9144426.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            42692..43489
                     /locus_tag="ACG8K3_000521"
     CDS             42692..43489
                     /locus_tag="ACG8K3_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP9144427.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            43692..44438
                     /gene="truA"
                     /locus_tag="ACG8K3_000522"
     CDS             43692..44438
                     /gene="truA"
                     /locus_tag="ACG8K3_000522"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9144428.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(44771..47440)
                     /locus_tag="ACG8K3_000523"
     CDS             complement(44771..47440)
                     /locus_tag="ACG8K3_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP9144429.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15300 a         8733 c        10156 g        13254 t
ORIGIN      
        1 aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg
       61 aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg
      121 ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa
      181 agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg
      241 ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac
      301 cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt
      361 ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa
      421 tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt
      481 tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac
      541 gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt
      601 atttatcttc ttttttacgc atactaaatc acctctatac attttactgt tcctatataa
      661 ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg
      721 aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat
      781 acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga
      841 gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc
      901 aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac
      961 atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg
     1021 tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg
     1081 tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag
     1141 ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat
     1201 atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct agacagacta
     1261 tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact
     1321 gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt
     1381 gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt
     1441 ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat
     1501 cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag
     1561 gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga
     1621 cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa
     1681 aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa
     1741 ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat
     1801 agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt
     1861 tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac
     1921 gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa
     1981 ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc
     2041 tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata
     2101 gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt
     2161 gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt
     2221 gtattattat aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat
     2281 aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata
     2341 tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg
     2401 gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat
     2461 tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta
     2521 aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca
     2581 aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact
     2641 aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt
     2701 gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat
     2761 aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc
     2821 tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa
     2881 ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca
     2941 gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt
     3001 ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa
     3061 gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg
     3121 tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt
     3181 gattttgact caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat
     3241 tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc
     3301 acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt
     3361 gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc
     3421 tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct
     3481 tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa
     3541 gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta
     3601 gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca
     3661 ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa
     3721 aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct
     3781 cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     3841 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     3901 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     3961 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     4021 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     4081 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     4141 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     4201 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     4261 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     4321 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     4381 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     4441 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     4501 cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     4561 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     4621 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
     4681 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
     4741 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
     4801 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
     4861 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
     4921 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
     4981 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
     5041 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
     5101 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
     5161 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
     5221 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
     5281 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
     5341 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
     5401 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
     5461 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
     5521 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
     5581 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
     5641 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
     5701 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
     5761 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
     5821 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
     5881 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
     5941 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
     6001 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
     6061 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
     6121 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
     6181 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
     6241 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
     6301 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
     6361 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
     6421 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
     6481 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
     6541 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
     6601 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
     6661 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
     6721 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
     6781 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
     6841 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
     6901 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
     6961 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
     7021 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
     7081 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
     7141 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
     7201 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
     7261 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
     7321 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
     7381 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
     7441 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
     7501 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
     7561 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
     7621 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
     7681 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
     7741 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
     7801 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
     7861 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
     7921 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
     7981 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
     8041 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
     8101 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
     8161 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
     8221 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
     8281 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
     8341 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
     8401 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
     8461 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
     8521 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
     8581 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
     8641 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
     8701 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
     8761 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
     8821 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
     8881 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
     8941 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
     9001 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
     9061 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
     9121 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
     9181 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
     9241 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
     9301 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
     9361 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
     9421 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
     9481 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
     9541 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
     9601 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
     9661 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
     9721 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
     9781 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
     9841 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
     9901 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
     9961 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    10021 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    10081 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    10141 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    10201 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    10261 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    10321 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    10381 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    10441 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    10501 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    10561 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    10621 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    10681 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    10741 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    10801 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    10861 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    10921 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    10981 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    11041 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    11101 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    11161 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    11221 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    11281 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    11341 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    11401 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    11461 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    11521 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    11581 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    11641 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    11701 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    11761 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    11821 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    11881 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    11941 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    12001 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    12061 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    12121 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    12181 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    12241 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    12301 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    12361 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    12421 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    12481 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    12541 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    12601 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    12661 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    12721 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    12781 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    12841 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    12901 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    12961 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    13021 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    13081 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    13141 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    13201 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    13261 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    13321 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    13381 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    13441 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    13501 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    13561 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    13621 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    13681 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    13741 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    13801 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    13861 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    13921 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    13981 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    14041 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    14101 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    14161 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    14221 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    14281 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    14341 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    14401 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    14461 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    14521 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    14581 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    14641 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    14701 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    14761 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    14821 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    14881 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    14941 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    15001 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    15061 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    15121 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    15181 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    15241 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    15301 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    15361 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    15421 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    15481 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    15541 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    15601 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    15661 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    15721 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    15781 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    15841 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    15901 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    15961 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    16021 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    16081 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    16141 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    16201 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    16261 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    16321 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    16381 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    16441 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    16501 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    16561 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    16621 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    16681 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    16741 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    16801 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    16861 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    16921 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    16981 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    17041 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    17101 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    17161 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    17221 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    17281 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    17341 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    17401 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    17461 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    17521 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    17581 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    17641 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    17701 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    17761 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    17821 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    17881 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    17941 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    18001 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    18061 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    18121 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    18181 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    18241 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    18301 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    18361 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    18421 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    18481 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    18541 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    18601 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    18661 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    18721 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    18781 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    18841 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    18901 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    18961 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    19021 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    19081 agaagttggc aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca
    19141 gtcccttttt tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt
    19201 taattcataa acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga
    19261 gataccttcg atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac
    19321 agttttgttg tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc
    19381 taatgtcact ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg
    19441 acggtcattt gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta
    19501 agaacgacgc cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt
    19561 caaaccttgt aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac
    19621 ccaaagttga tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac
    19681 agaagtgtaa gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt
    19741 tgcttcttcg attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa
    19801 tgcattccat tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc
    19861 ttttaagata ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg
    19921 aagagatttc catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa
    19981 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
    20041 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
    20101 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
    20161 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
    20221 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
    20281 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
    20341 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
    20401 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
    20461 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
    20521 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
    20581 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
    20641 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
    20701 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
    20761 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
    20821 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
    20881 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
    20941 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
    21001 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
    21061 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
    21121 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
    21181 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
    21241 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
    21301 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
    21361 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
    21421 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
    21481 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
    21541 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
    21601 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
    21661 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
    21721 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
    21781 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
    21841 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
    21901 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
    21961 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
    22021 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
    22081 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
    22141 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
    22201 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
    22261 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
    22321 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
    22381 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
    22441 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
    22501 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
    22561 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
    22621 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
    22681 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
    22741 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
    22801 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
    22861 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
    22921 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
    22981 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
    23041 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
    23101 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
    23161 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
    23221 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
    23281 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
    23341 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
    23401 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
    23461 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
    23521 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
    23581 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
    23641 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
    23701 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
    23761 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
    23821 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
    23881 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
    23941 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
    24001 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
    24061 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
    24121 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
    24181 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
    24241 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
    24301 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
    24361 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
    24421 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
    24481 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
    24541 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
    24601 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
    24661 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
    24721 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
    24781 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
    24841 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat
    24901 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
    24961 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
    25021 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
    25081 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
    25141 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
    25201 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
    25261 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
    25321 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
    25381 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
    25441 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
    25501 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
    25561 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
    25621 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
    25681 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
    25741 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
    25801 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
    25861 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
    25921 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
    25981 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
    26041 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
    26101 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
    26161 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
    26221 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
    26281 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
    26341 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt
    26401 attttctaaa tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg
    26461 tgagtttata tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta
    26521 atcagaatga tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca
    26581 cgaacgagcg tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga
    26641 gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt
    26701 ttagatcaat cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct
    26761 ggtccaattc cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa
    26821 tacaaagatt cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg
    26881 aacccaacac caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac
    26941 atcgaaatca aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga
    27001 atcttaggga aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca
    27061 gtaactgttg tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat
    27121 ggctacgaag ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct
    27181 gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa
    27241 aatgttgagc taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca
    27301 ggagacatta ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa
    27361 cgccacggac aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg
    27421 tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg
    27481 ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat
    27541 gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca
    27601 gctgtgaaag ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca
    27661 ttattcaaac aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga
    27721 attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga
    27781 caaggaacac acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg
    27841 cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga
    27901 ggtggcgtag tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt
    27961 cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca
    28021 atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac
    28081 ttatctatcg actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta
    28141 tcagcacgta acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac
    28201 gttgtatcaa atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg
    28261 cttgcataat gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc
    28321 ttgccatgga tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag
    28381 taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg
    28441 tacgtgcgaa attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag
    28501 cgatcgttac attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag
    28561 cactaataga taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc
    28621 tacctcaaat ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac
    28681 acctgaaaaa tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg
    28741 catcactgta cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa
    28801 acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc
    28861 agctaacatc gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa
    28921 aggactagaa gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa
    28981 tgctcttcca ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc
    29041 tggtaaaggt ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga
    29101 aggcaaatac gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg
    29161 ccgtgctaca atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc
    29221 tggacgctct cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa
    29281 tgatcaccca cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc
    29341 tccttggggt caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa
    29401 acttattgtt cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga
    29461 gaggaggttc accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat
    29521 gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg
    29581 ccgttcaaca gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa
    29641 acatgtacca gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc
    29701 tacaagaact tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg
    29761 gaggataaat ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc
    29821 gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag
    29881 atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat
    29941 tgatgtcagc agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag
    30001 taagcgaagc ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag
    30061 gatcagcttc tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat
    30121 aaggagggat aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc
    30181 cgcgactggg atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat
    30241 ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa
    30301 atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt
    30361 attggtaaag gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt
    30421 aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta
    30481 ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa
    30541 gccatccaac gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt
    30601 ctaaacggag cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat
    30661 actttacgtg cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta
    30721 ggagttaaag tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa
    30781 ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa
    30841 aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag
    30901 ctgttgattc acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc
    30961 gttacatgaa acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg
    31021 ccaaagcaat tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac
    31081 cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg
    31141 aagcgttacg tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg
    31201 aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg
    31261 cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca
    31321 ggtcaattag aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa
    31381 actgtgttgc gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa
    31441 ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca
    31501 attaccgttg ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat
    31561 tctaagaaat acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga
    31621 attatggaaa ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa
    31681 gaagcagtta ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa
    31741 aggaggatac acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc
    31801 gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc
    31861 ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt
    31921 gacgtcgtta aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca
    31981 tatattaaat ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga
    32041 acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt
    32101 tcactagcac cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt
    32161 atgtttgtta aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa
    32221 ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc
    32281 gttaaaaaac accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa
    32341 gcgccaattc atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt
    32401 ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta
    32461 gataaataaa agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat
    32521 cttaaagaag taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca
    32581 cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa
    32641 aacttagata aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca
    32701 aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa
    32761 gttacattgc gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta
    32821 ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat
    32881 actttaggta ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa
    32941 gtacgtggta tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag
    33001 ttgttgacac aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct
    33061 aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact
    33121 cgttgcgaac gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt
    33181 tgcttccgcg aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa
    33241 aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag
    33301 attttctaac tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg
    33361 catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg
    33421 ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa
    33481 acggcgaacg tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg
    33541 tcaaagctga cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt
    33601 ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg
    33661 cttacgtatg gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga
    33721 aataaagtcg ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca
    33781 gttaaaggac caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc
    33841 gaaggaaacg aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat
    33901 ggaacaactc gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa
    33961 gcgttagaac taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg
    34021 aacgttggtt actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta
    34081 ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct
    34141 gcaaacattc gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt
    34201 ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca
    34261 tagaagccag cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt
    34321 ttacttgtgc cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa
    34381 taaaaataaa gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa
    34441 gagacatcgt cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt
    34501 ttttcgttct aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct
    34561 agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca
    34621 agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt
    34681 tgaccgtggt ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga
    34741 aaatggacta gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa
    34801 catttggaat tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt
    34861 ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg
    34921 tttggtacag gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg
    34981 aagaaaaact tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt
    35041 gcttttagtg gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct
    35101 ggtggaccag ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct
    35161 ttaggctcaa atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg
    35221 aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa
    35281 ggaggacaac aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc
    35341 ctcaaaacca acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg
    35401 ttaagccttc taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg
    35461 ttgaagaaat ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg
    35521 aaacttcatg aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt
    35581 ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt
    35641 tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta
    35701 ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta
    35761 ttgaaccgct ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc
    35821 gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta
    35881 actgtgaaag cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga
    35941 tcaatcgagg tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta
    36001 gatcgaaaat cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta
    36061 ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca
    36121 tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt
    36181 caccatacat tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat
    36241 ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat
    36301 atctgacatt agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat
    36361 acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga
    36421 ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta
    36481 tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta
    36541 tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg
    36601 ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt
    36661 tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta
    36721 caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta
    36781 gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg
    36841 aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga
    36901 tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg
    36961 agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta
    37021 aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct
    37081 tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat
    37141 caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga
    37201 aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg
    37261 tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac
    37321 ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat
    37381 gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa
    37441 acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa
    37501 gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta
    37561 ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat
    37621 ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa
    37681 cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac
    37741 cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa
    37801 gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca
    37861 atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc
    37921 gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg
    37981 acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc
    38041 gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt
    38101 tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa
    38161 agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa
    38221 agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca
    38281 ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa
    38341 ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt
    38401 atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt
    38461 aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa
    38521 ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg
    38581 tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag
    38641 ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg
    38701 ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact
    38761 tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat
    38821 tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg
    38881 ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa
    38941 aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc
    39001 attctacatt caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt
    39061 ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc
    39121 aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag
    39181 taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag
    39241 gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc
    39301 caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg
    39361 aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca
    39421 agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa
    39481 agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca
    39541 ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa
    39601 ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg
    39661 tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct
    39721 ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt
    39781 gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt
    39841 caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc
    39901 tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa
    39961 gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca
    40021 atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat
    40081 gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg
    40141 gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca
    40201 ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt
    40261 aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt
    40321 ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa
    40381 ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta
    40441 atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa
    40501 aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc
    40561 gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct
    40621 ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca
    40681 cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt
    40741 gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt
    40801 ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag
    40861 taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt
    40921 tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata
    40981 gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga
    41041 aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt
    41101 tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac
    41161 acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa
    41221 tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa tgatttttca
    41281 aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga
    41341 aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt
    41401 tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg
    41461 tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac
    41521 cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga
    41581 agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa
    41641 tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt
    41701 ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa
    41761 gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat
    41821 ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca
    41881 ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact
    41941 gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt
    42001 gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat
    42061 aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca
    42121 cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc
    42181 agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa
    42241 agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc
    42301 gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt
    42361 gatccccagg gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag
    42421 atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc
    42481 tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac
    42541 attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa
    42601 ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac
    42661 gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta
    42721 tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt
    42781 ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt
    42841 tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat
    42901 tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg
    42961 cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg
    43021 cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac
    43081 tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt
    43141 acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac
    43201 attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg
    43261 tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt
    43321 cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg
    43381 tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat
    43441 cattgctttt gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg
    43501 gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga
    43561 caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa
    43621 gaaagaacga aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag
    43681 gagagaaaaa catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg
    43741 gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa
    43801 aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac
    43861 atgcgatcgg gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc
    43921 gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt
    43981 cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata
    44041 tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg
    44101 atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt
    44161 tttgtgcatc aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg
    44221 aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata
    44281 agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag
    44341 atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc
    44401 ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca
    44461 gcttcaagaa ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg
    44521 cttattttcg aagaagctac ttctggaaca ccgtttaaga aagaggtcgt gagcctgtca
    44581 tttaactctg agtcacaaag agcaatttca aaaaacagct tctcatcttt gatgaatatc
    44641 gtgacttgtg gacgaggcgt tgataagtga atcatctttt tgttacctat tttttctgta
    44701 accaaaaaac gatggaacgt tgttttgatt gcccttggtg cggacagtct gaacaggttc
    44761 catcgtttct ttattttcta cgtcttcttc tgtgtgtgtt tcgtggttgt tcttctattg
    44821 ctggatcaat cgtgccataa aaggggcgat tgcggtcaag gtaccagaca taaagaataa
    44881 acaatgcaat acataggaca aaaaggattg cacctatcgt aaatccttca ggcaagtaac
    44941 tgaaagtgac agtatgttta cctgctggaa cttttatcat caagaatgcg tctttgaaat
    45001 tgctgacctt gactggtttc ccatcaattt tagctttcca tcctttgtca taaggaatag
    45061 ttgtcaccaa catggaatct tcagcggtat ttacagtccc agaagctttt ctgccagtgg
    45121 cttccatttc gacacctttt tctttgatct gattcattgc ggcttgatag gctacaacat
    45181 ctagaccaac gactttaggc tccatgaaac ttatttcttt cgtcccataa aaacttgcag
    45241 taaatgtcac tgttgtctct tgaggataat agccaagatc atagtattga ccagaaatgt
    45301 tgatttgcgt ttttcgcgat gtaccattga cagtgatcgt cgcactagaa ctttctaatt
    45361 gcgcaaaatt ggttgggaac aaacttaagt aagcttgtgt atttgcaggc acatggactt
    45421 cccaagtgag ctcttttggc tggttatctg ctgtttctga gtaagtgacg ccagtcgttg
    45481 tctttgtgat cgtcgtattc aactgattct tcaaagtagg tgtatagaat gtaaaatact
    45541 gttcatttag accagacaat gtattgatca agtttgtctg actggtcaaa ttatctgtca
    45601 ctggctgctc aacatcatta acggacaacg gtgcagtaaa tgctagtggc aatgcattga
    45661 cattctgata cagtgtatag tcgccgcttt gaccagcttg gcggaagcca tatttactga
    45721 acgtgctttc gtttttagaa atgttatatt tgattcctgt aaacccatcc atcagcaaag
    45781 tattattgtt ataacggata ttcaaatttg tccctcttga acggaaacct aatttatcca
    45841 ggtaagagga agaattacga tttcttatag aagaaaacaa actgatacca ctgtaaccat
    45901 agttgatcga atcgtttgca gatactggat ccagattttc caagcggtaa aacgagtcgg
    45961 aatctttttt tgtttcattt acaagagctt tcaagtcagg ataaggatca gtatataaac
    46021 tgcgggaagc ataattccaa tcatcaagaa tgccatggat catactgttc gtattgacca
    46081 gtgcttcagc agacatgatc aataataaca atatactcag atgattgaat gaaagttttt
    46141 tatgctgata aaatcccaca gcaaaagcat aaatccctaa gaaaaccact gtcaatacaa
    46201 aggaagtcat cgtcacatag gtgtagcttc cttcaggttt gatgccccat gccaccgcaa
    46261 agagagcagc tacgaccaaa atgattccta acaacagccc accatcgtct tgttctatct
    46321 tttcccatcc ttttcctgca agcaggagca ctaagaaaga gaacagatag gcataacgga
    46381 ataaaaacat attcggtgca tgcataccat gccagaacaa attcaatgga acaagataaa
    46441 aacttgcgat aagtatggcg aaaagactgc cgtacaaaaa tttatttttc aacggtactt
    46501 tccgactggc aaaataaaac agacagagga ctaatggtag cagacctgcg taaacgaacg
    46561 gaatcgatcc gtattttgtc gtgtcataaa caccaatcat atttttcatg attagatcca
    46621 gataagaagt cgcttctgtt ttcaacgtag tgatctgact cagtgcctcg ccatttgacc
    46681 gaagatccag cactgctggc aatacaatga tcattgaagc gccgcctgcc agtaaagaag
    46741 tgataccata aggaacaatc gtttttttat attctgacca gtttgtcaat agacgggcga
    46801 taaaatataa gaaagaaaat acgccaatca taaaacccat ataaaaactg gataaaaaca
    46861 agagtaaata actgataaat aaaagtttcg gccgtttaag atccataaca cgatgaatcc
    46921 ccaaaatcac taatggaaga taaacaaatg catccagcca catgatgatc tccgaatgtg
    46981 cagtgataaa tgagatcaat gcgtaactga cgctaagtgc aacatgcccc cagcgaggaa
    47041 tcttgaacgt acgtgcaaga aaccaaaaag ccaatccggc acagccgacc ttaagcaaag
    47101 tcagaacata cagtgcatct ggcatcatct ggtttggaaa aaagaacacc agtggcgtga
    47161 agatcccgcc tagatagtaa gaaattaaag ataaataatt caatcctaga gaagcattcc
    47221 atgtataaaa aatactttgc tttccaagaa gaacatttcg gaagctggca tgaaaattag
    47281 aaaactgtga aaaggcatca cttgccaaaa tacttctgct gcttccagga tagattccga
    47341 tacttagata aacaagtgct aaaatcaaaa acggaatcag gaaactagct gctgcgtagc
    47401 gtccgttttc tctaaagaat gactttaaat tttttttcat att
//
LOCUS       DAZKCN010000010        43070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000010 DAZKCN010000000
VERSION     DAZKCN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.010"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..253
                     /locus_tag="ACG8K3_000524"
     CDS             23..253
                     /locus_tag="ACG8K3_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP9144430.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            350..1651
                     /gene="murA"
                     /locus_tag="ACG8K3_000525"
     CDS             350..1651
                     /gene="murA"
                     /locus_tag="ACG8K3_000525"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9144431.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1664..1840
                     /locus_tag="ACG8K3_000526"
     CDS             1664..1840
                     /locus_tag="ACG8K3_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9144432.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1975..2271
                     /gene="yidD"
                     /locus_tag="ACG8K3_000527"
     CDS             1975..2271
                     /gene="yidD"
                     /locus_tag="ACG8K3_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9144433.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2455..2922
                     /locus_tag="ACG8K3_000528"
     CDS             2455..2922
                     /locus_tag="ACG8K3_000528"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144434.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3007..3891
                     /locus_tag="ACG8K3_000529"
     CDS             3007..3891
                     /locus_tag="ACG8K3_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9144435.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4005..4241
                     /locus_tag="ACG8K3_000530"
     CDS             4005..4241
                     /locus_tag="ACG8K3_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP9144436.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4329..4409
                     /locus_tag="ACG8K3_000531"
     tRNA            4329..4409
                     /locus_tag="ACG8K3_000531"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4363..4365,aa:Tyr,seq:gta)
     gene            4416..4487
                     /locus_tag="ACG8K3_000532"
     tRNA            4416..4487
                     /locus_tag="ACG8K3_000532"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4448..4450,aa:Gln,seq:ttg)
     gene            4612..4782
                     /locus_tag="ACG8K3_000533"
     CDS             4612..4782
                     /locus_tag="ACG8K3_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144437.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5002..5709
                     /locus_tag="ACG8K3_000534"
     CDS             5002..5709
                     /locus_tag="ACG8K3_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9144438.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5769..6413)
                     /locus_tag="ACG8K3_000535"
     CDS             complement(5769..6413)
                     /locus_tag="ACG8K3_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP9144439.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6540..6827
                     /locus_tag="ACG8K3_000536"
     CDS             6540..6827
                     /locus_tag="ACG8K3_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144440.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6935..7255
                     /locus_tag="ACG8K3_000537"
     CDS             6935..7255
                     /locus_tag="ACG8K3_000537"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9144441.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7336..8649
                     /locus_tag="ACG8K3_000538"
     CDS             7336..8649
                     /locus_tag="ACG8K3_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9144442.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8646..9365
                     /locus_tag="ACG8K3_000539"
     CDS             8646..9365
                     /locus_tag="ACG8K3_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9144443.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9493..10044
                     /gene="raiA"
                     /locus_tag="ACG8K3_000540"
     CDS             9493..10044
                     /gene="raiA"
                     /locus_tag="ACG8K3_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9144444.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10266..12800
                     /gene="secA"
                     /locus_tag="ACG8K3_000541"
     CDS             10266..12800
                     /gene="secA"
                     /locus_tag="ACG8K3_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9144445.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            12927..14028
                     /gene="prfB"
                     /locus_tag="ACG8K3_000542"
     CDS             join(12927..12998,13000..14028)
                     /gene="prfB"
                     /locus_tag="ACG8K3_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9144446.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14133..14819
                     /gene="ftsE"
                     /locus_tag="ACG8K3_000543"
     CDS             14133..14819
                     /gene="ftsE"
                     /locus_tag="ACG8K3_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9144447.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14812..15696
                     /gene="ftsX"
                     /locus_tag="ACG8K3_000544"
     CDS             14812..15696
                     /gene="ftsX"
                     /locus_tag="ACG8K3_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9144448.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            15848..17296
                     /gene="cls"
                     /locus_tag="ACG8K3_000545"
     CDS             15848..17296
                     /gene="cls"
                     /locus_tag="ACG8K3_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9144449.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            17633..18490
                     /locus_tag="ACG8K3_000546"
     CDS             17633..18490
                     /locus_tag="ACG8K3_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9144450.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18605..19525
                     /gene="pstC"
                     /locus_tag="ACG8K3_000547"
     CDS             18605..19525
                     /gene="pstC"
                     /locus_tag="ACG8K3_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP9144451.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19525..20409
                     /gene="pstA"
                     /locus_tag="ACG8K3_000548"
     CDS             19525..20409
                     /gene="pstA"
                     /locus_tag="ACG8K3_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9144452.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20424..21230
                     /gene="pstB"
                     /locus_tag="ACG8K3_000549"
     CDS             20424..21230
                     /gene="pstB"
                     /locus_tag="ACG8K3_000549"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9144453.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21246..22004
                     /gene="pstB"
                     /locus_tag="ACG8K3_000550"
     CDS             21246..22004
                     /gene="pstB"
                     /locus_tag="ACG8K3_000550"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9144454.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22017..22694
                     /gene="phoU"
                     /locus_tag="ACG8K3_000551"
     CDS             22017..22694
                     /gene="phoU"
                     /locus_tag="ACG8K3_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9144455.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23258..23950
                     /locus_tag="ACG8K3_000552"
     CDS             23258..23950
                     /locus_tag="ACG8K3_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9144456.1"
                     /translation="MLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAIL
                     FVVIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVI
                     GSMANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIA
                     VLFFLAVRRLKK"
     gene            23947..24714
                     /locus_tag="ACG8K3_000553"
     CDS             23947..24714
                     /locus_tag="ACG8K3_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP9144457.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            24814..26382
                     /gene="liaX"
                     /locus_tag="ACG8K3_000554"
     CDS             24814..26382
                     /gene="liaX"
                     /locus_tag="ACG8K3_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP9144458.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26558..26866
                     /locus_tag="ACG8K3_000555"
     CDS             26558..26866
                     /locus_tag="ACG8K3_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP9144459.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            26866..27216
                     /locus_tag="ACG8K3_000556"
     CDS             26866..27216
                     /locus_tag="ACG8K3_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9144460.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            27382..28320
                     /gene="hprK"
                     /locus_tag="ACG8K3_000557"
     CDS             27382..28320
                     /gene="hprK"
                     /locus_tag="ACG8K3_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP9144461.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28335..29165
                     /gene="lgt"
                     /locus_tag="ACG8K3_000558"
     CDS             28335..29165
                     /gene="lgt"
                     /locus_tag="ACG8K3_000558"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9144462.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            29209..30234
                     /locus_tag="ACG8K3_000559"
     CDS             29209..30234
                     /locus_tag="ACG8K3_000559"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9144463.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30249..31187
                     /gene="galU"
                     /locus_tag="ACG8K3_000560"
     CDS             30249..31187
                     /gene="galU"
                     /locus_tag="ACG8K3_000560"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9144464.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31312..31761
                     /locus_tag="ACG8K3_000561"
     CDS             31312..31761
                     /locus_tag="ACG8K3_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP9144465.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            31754..32368
                     /locus_tag="ACG8K3_000562"
     CDS             31754..32368
                     /locus_tag="ACG8K3_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9144466.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32431..33534)
                     /locus_tag="ACG8K3_000563"
     CDS             complement(32431..33534)
                     /locus_tag="ACG8K3_000563"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP9144467.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            33744..34745
                     /gene="ccpA"
                     /locus_tag="ACG8K3_000564"
     CDS             33744..34745
                     /gene="ccpA"
                     /locus_tag="ACG8K3_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP9144468.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35099..35494
                     /locus_tag="ACG8K3_000565"
     CDS             35099..35494
                     /locus_tag="ACG8K3_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144469.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            35491..36747
                     /locus_tag="ACG8K3_000566"
     CDS             35491..36747
                     /locus_tag="ACG8K3_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFP9144470.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            36759..37082
                     /locus_tag="ACG8K3_000567"
     CDS             36759..37082
                     /locus_tag="ACG8K3_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144471.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            37075..37341
                     /locus_tag="ACG8K3_000568"
     CDS             37075..37341
                     /locus_tag="ACG8K3_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144472.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(37504..39849)
                     /locus_tag="ACG8K3_000569"
     CDS             complement(37504..39849)
                     /locus_tag="ACG8K3_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP9144473.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            40128..41030
                     /locus_tag="ACG8K3_000570"
     CDS             40128..41030
                     /locus_tag="ACG8K3_000570"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9144474.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            41241..43016
                     /locus_tag="ACG8K3_000571"
     CDS             41241..43016
                     /locus_tag="ACG8K3_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP9144475.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLKLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT        14194 a         7519 c         9240 g        12117 t
ORIGIN      
        1 aatcgttaga aaggacgtca ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt
       61 gagtcatttg gcgtttatct acttagcctt ctggtcttta cgttcgttac gcattgaaac
      121 ttttttcaga ccaatgcata atacgcaaat tagaatggca atcgtacttt tttcaatttt
      181 tttagggtac actgcaagta attttttctt agaagttatc gctttatgcc gaaatctttt
      241 cgttagcctg taaattttaa aaaaaggtac tattttaaaa gcaaggatgt tataatagaa
      301 aagattttga gtaagtgtat gctcattgga attttttgga gggaacaaca tggaagagat
      361 catcgtaaga ggtggcaatc aattaaatgg aacagtaaga attgaaggag ctaaaaatgc
      421 tgttttgcct attttagctg ctagtttgtt agcagaagaa ggtattacga cattagacaa
      481 cgttccgatt ctttctgatg tcttcactat gaatcaagtg attcgtcact taaacgtaga
      541 tgtagatttt gatgaacaaa agaatcaagt aacaatcgat gcttcaagac aattagaaat
      601 cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct
      661 acttgccaga aatggacatg caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg
      721 accaatcgat ttgcatttaa aaggatttca agctttagga gcaaaaatta ttcaaaaaaa
      781 cggctatatc gaagcaattg cggatgaatt gatcggtaac acgatttatt tagatttccc
      841 tagtgtaggt gctactcaaa atatcatgat ggctgccgta aaagcaaaag gaacaaccat
      901 cattgaaaac gtagctcgcg aaccagaaat cgttgattta gcaaacattt tgaataaaat
      961 gggcgctcaa gtctatggag ctggtactga aacaatgcgt attgaaggcg tggatcattt
     1021 acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc
     1081 tgctgcaatg acacaaggca atgtgctgat tgcagatgct atctctgaac ataaccgccc
     1141 attgatttct aaattgatcg aaatgggtgc agaaatcatt gaagaagaag gcggagtccg
     1201 tgtcatcgga ccaaaacata tcttacctac agatgtaaaa acgatgcctc atcctggttt
     1261 ccctacagat atgcaagctc aaatgacagc tatccaatta gttgcagaag gtacaagtgt
     1321 agtgactgaa acagtgtttg aaaatcgttt ccaacattta gaagagatgc gccgtatgaa
     1381 cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg aattgcaagg
     1441 tgcagaagta tatgcaacag acttgcgggc agcagcagca ttagttttag ctggcttgaa
     1501 agcaaatgga atcacacgtg tgcgtaactt gaattatcta gaccgtggat attataattt
     1561 ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt gtagacatgg atcaaacttc
     1621 tgctgaaaaa acagcacaaa cgattgctta aggaagtggc tgaatgagta accaacgata
     1681 tatcatatta actttgataa agattttagt ggtgatctta ttgcttatcc ttctttttgt
     1741 ggccggaacg atgatcggat atggtgtcat cggtggcggc aatccattta aagtattcca
     1801 gccaagtttg tggattcata ttcgagactt ttttcattga taaataagaa tcacagtggt
     1861 tctagtcgat atgaagtgag ccatgaagca gaacatgaca aaagaaaaca gaagggaacg
     1921 ttgacgttag tcgcgatccc tttttttagt aaaagaaaag aaggaatcta attgatgaag
     1981 aagatattga tttatttagt tagaggatat cagcgcttta tttcgcctgc atttccacct
     2041 agctgccgtt attacccaac ctgttccaat tatatgataa aagcaattca aacccatggt
     2101 gcactaaaag ggacggtaat ggggactgca cggatcttac gctgtcatcc ttttgtcaaa
     2161 ggcggtatcg attatgtacc attgacattc caattaacgc gaaataagaa agataaaaaa
     2221 gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca ctgagcgata aagaaacaat
     2281 ttggaaaata gcttcttata ttttgcaaaa ttgtgagtta ttgccgaagt gttggatttt
     2341 gaacaccgtc aaaaaaaggt tgtgaaaaat acgggtagca ctgagcgata aagaaaaaga
     2401 ggaaaattat tgtcaaagta acaactccgc aactacttat atgggggaca aaaaatgatc
     2461 gaagaactaa acaagaaaga tttaccatgg tccttgttat tagatgcaga tcctgataaa
     2521 gaaaaagttc aagtatatgt ttccagaggg agtggctgga tatggaaaga aaaagaaaaa
     2581 acaataggtg tactgatcta tgtggcaagg gaaaaagaat acgaaatcgt caatgtagca
     2641 gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat tacttgagac agcttttcag
     2701 aagctttcac aactcgcttc cagtcaaact cagatcatca tacgaaccgg cagtactagc
     2761 agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg agattggtcg agtcaaagac
     2821 tattttatcc atcattactc tgagcccatt tttgaaaatg gagatcaatt aagggatcaa
     2881 gtaattttag caattcactt acctaaaaat agctttaact aataaacttt caattcgaga
     2941 aaatgctttc aaatgctaaa tagatacatt acaatagctt tagcaaacaa aaaggagaat
     3001 atttttatgg gaatattagt ttttgatttt ggtggatcgg ctgtcaaata tggttgttgg
     3061 gatggcaaag agattaaagg taagggacaa tttgcaacgc ctgaatcttg ggaagaaatg
     3121 aaagcgcaat tatttcaagt ttataaaaaa atactcgttt cttttgaagt cgaaggaatt
     3181 ggaatcagct ctcctggagt agtgaataat gagaaacagg tgattgaggg aatcagtgcg
     3241 attccttata ttcatggatt taatattttc cgagatttgg aaactctgtt tcaactccca
     3301 gtaacgattg aaaatgacgc caactgtgcc ggaatggctg aattttatga aggtgctgcc
     3361 cgtgattatc aagatgttgc ttttgttgtg gcaggaactg ggattggcgg ggcgttgttc
     3421 acgaaaggac aaatcaataa aggggcacat ctttatggtg gagaatttgg gttgatgttc
     3481 ttaaatggcg ataagacatt cagtaaactt ggtacagcgg tacagatggc ttggcgttat
     3541 tgtgaacgca aaggtctcga taaaaattct tataccggaa aagatgtctt tgaattagca
     3601 gaaactggag atgcaatagc aaaagaagaa gttgagagtt tttatacgta tcttacaaag
     3661 ggattattca gtattcaatt ttcgtttgat ccagaagtga tcgtattagg cggcggtgtt
     3721 tctgcaaaag aagggctcat tgatgagatc aaaagccgaa tgaagaagct gacagaacaa
     3781 ttcgatctgc atgatttcga accgcagatt ttattatgtg aatacagaaa cgatgctaat
     3841 cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag gggagaatta agagctttag
     3901 agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat aggagatttt tacctaagga
     3961 atgttttatt tgctgcagta tgataaaata tctgagaggt gaaaatgatg tcaaaaaaaa
     4021 ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga tatcaaaggt aagaattatg
     4081 aagtcactca gttatttata gggaaaaaaa tgattggtga gatcttagcc tatgggccaa
     4141 aagaatatga aattttcttt ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg
     4201 cgatagaaac agtgattcga cactggaatc tgcacgaata aaaaaatatc aaaaaaactt
     4261 tcaataaagg gttgcaaaaa tctggaaaat taggtattat aactaagtca ggttgataca
     4321 aacctttcgg aggagtagcg aagtggctaa acgcgacgga ctgtaaatcc gttccttagg
     4381 gttcagtggt tcgaatccac tctcctccat ttcattgggg tatagccaag cggtaaggca
     4441 agggactttg actccctcat gcgttggttc gaatccagct accccagtat accacaatcg
     4501 taagctctcg ttagttcgag agctcttttt tttgctttgc taccttgttc cttgaagatt
     4561 ctgctgattt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
     4621 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
     4681 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
     4741 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
     4801 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
     4861 aaaattgttt ctgacaatag gagtaggaag aacgatgtgt gtctaaatcc tctgttgatg
     4921 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
     4981 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
     5041 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
     5101 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
     5161 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacatatgt tgcgcgagaa
     5221 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcgcaaaat
     5281 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
     5341 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
     5401 gatgatgtcc ctatttgttt tgaagttgcc agtgtgccac aaaagctgat cgatggctac
     5461 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
     5521 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
     5581 ttagaggtaa aacgaggaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
     5641 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
     5701 gaaaaataaa aaacaggtga tccctgattc cttacttaga aataaaagag aatcagaatc
     5761 acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg accctcttca
     5821 aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt
     5881 tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa
     5941 tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag
     6001 atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagacagca
     6061 tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg
     6121 actactgcag ctacattgat cagttgattt tttttgagga cttccaacat cggtacgcca
     6181 gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg
     6241 atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa
     6301 ctttttgctt ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg
     6361 atctcaatct cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca
     6421 cctctttttt tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt
     6481 aacggttgcg caatcgctgt agtatgcgat aataaagaca aaggtaggtg gatctgggga
     6541 tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct
     6601 ctcaagtaac gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc
     6661 ccaaaacagc aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag
     6721 gtgcctatgg ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat
     6781 atgtcgtgtt caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt
     6841 agaaaacgtt gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg
     6901 tttgcgtagt ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt
     6961 atgttcggct ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc
     7021 aaaaggaatc gactcggata ttttcgctgt gtccgcttct gacgcagata acaatcttgc
     7081 aaataaagat gtagatgtac tgttattagg acctcaagtt cgctttatga aagctgattt
     7141 tgaaaaacgt ctggaaccaa aaggcattcc tttagacgtc atcaatatgg ctgattatgg
     7201 tatgatgaat ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga
     7261 acagtataac tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt
     7321 tttagggcga ataatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattaaa
     7381 gcaatagact gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt
     7441 ttgggcaact ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag
     7501 cttgcagatt acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa
     7561 caagatttat atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg
     7621 acaggacagc ttactgaaaa acaaacggaa atcgcaaaga gaatactttg ccattcagaa
     7681 aaaagacatc atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct
     7741 attttagtgg aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat
     7801 gtttgtaatg aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg
     7861 ctccatggga atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat
     7921 cagctatacc gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt
     7981 ccttataacg gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga
     8041 cgattcatct acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg
     8101 tttgacatcc acaaacttgc tttgcgcttt catcggcgtt tacttcctga acccgtcttg
     8161 gttttcatga ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg
     8221 cggatcattc atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg
     8281 atgatgcatc gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat
     8341 gtttcttcaa gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat
     8401 gacatactgt taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt
     8461 atcgttttag gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga
     8521 agagccgatc gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa
     8581 actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag
     8641 atgctgtgag gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca ttcagcgaga
     8701 ttattggtgt ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc
     8761 atttattgga aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga
     8821 gtacgaaaaa gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga
     8881 agaaatatcc gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga
     8941 aggcctggct caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat
     9001 ataaatggac gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat
     9061 ctgaaaaaag gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
     9121 gaattccttt tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca
     9181 cgcgaaagga aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga
     9241 tacaaaataa aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc
     9301 acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttagcaa
     9361 gatgaattag atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata
     9421 taatagaatt aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa
     9481 ggggtaattt ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg
     9541 attcgcgact acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc
     9601 gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta
     9661 acgatcccat taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca
     9721 agtatcgatt tggttgttga caaattggaa cgccaaatcc gcaaattcaa aacaaaaatt
     9781 aatcgtaaat cccgtgaaac gggcatgaat actgcagatg ctactgcttt attcaatgaa
     9841 gaggacgaaa acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg
     9901 aaaccaatgg atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt
     9961 atttttgaag atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa
    10021 tacggactga ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca
    10081 gctactgaca gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat
    10141 aaattatgaa gttcttactt taatacgctt aagatatttc aattatctat aactaatgat
    10201 aaaatgaaaa gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac
    10261 aacgcatggc caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt
    10321 tagatagcat tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg
    10381 aacaattaca ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag
    10441 atcaattact tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt
    10501 tatatccata ccacgtacaa ttaatgggtg ggatcgtgct tcacgatggg aatatccctg
    10561 aaatgcgtac tggtgaaggt aaaaccttga ctgcaacgat gccggtgtat ctgaatgcgt
    10621 tgactggcga aggggtccac gtagttaccg tcaatgaata tttagcaacc cgtgactcga
    10681 cggaaatggg cgagctttat aatttcttag gattgacagt tgggttgaat atcaactcaa
    10741 aatcttcgga tgaaaaacgc gaggcttata attgcgatat cacttatagc acgaataatg
    10801 aattaggatt tgactatttg agagacaaca tggtcgttta ccgtcatcaa atggtacaac
    10861 gtccgctgaa ttatgccatt gtcgatgaag ttgactcgat cttgattgat gaagcgagaa
    10921 cgccgctgat catttcaggg caagcagaaa aatcaacagc tttatataca agagcagaca
    10981 acttcgtgaa acgtttgaaa gaagaagaag attataaaat cgatatacaa tcaaaaacaa
    11041 tcagtttaac tgaagcaggg atcgaaaaag ctgaagaaac attcggtttg gacaacttat
    11101 atgacattga aaatactgca ttgacccatc acttggatca agcattgaga gccaactaca
    11161 tcatgctttt agacatcgat tatgttgttc aagatggaaa agtattgatt gttgaccaat
    11221 ttacgggtcg gatcatggat ggacgccgtt attcagatgg tcttcatcaa gcgatcgaag
    11281 ctaaagaagg cgtagagatc gaagacgaaa cgaaaacgat ggcgacgatc actttccaga
    11341 actatttccg tatgtacaag aaacttgcgg ggatgactgg tacagctaag acagaagaag
    11401 aagagttcag agagatttat aatattcaag tataccagat tccgacaaac cgtccggtca
    11461 tccgtgacga tcgaccagat ttgctttacc caacattaca aagcaagttc catgctgtcg
    11521 tacaagatat caaagatcgc taccgcaaag gacagcctgt cttagtcgga actgttgcag
    11581 ttgaaacatc tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtac
    11641 tgaatgcgaa aaatcacttt aaagaagcag aaatcatcat gaatgcgggt caaaaaggtg
    11701 cagtgacgat tgctaccaac atggctggtc ggggaacaga tatcaagcta gggcttggcg
    11761 taagagaact tggtggccta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg
    11821 ataaccagct tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact
    11881 tgtctttaga agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag
    11941 atcgaatgaa gatcggtgac gaagatgctg tgatccaaag caagatgctg acgaaacaag
    12001 tagaagctgc acaaaaacgc gtagaaggaa acaactacga cacacgtaag aacgtcttgc
    12061 aatacgatga cgtgatgcgt gagcaacgtg aagtaatcta taaacagcgt caagaagtga
    12121 tcatggaaga aaaaagctta tctaaaccac tgatgaatat ggtcaaacgg acaatcagtc
    12181 gtgtggtaga tgcgcatacg caattagaaa aagaaaattg gaatttggaa agtatcgtcg
    12241 attttgctgg cagcacgctt gtccatgaag acagtatttc ccttacagat atcgaaggaa
    12301 aaacctctca agaaatcaaa gaatatctaa acgaacgagc acaagaagta ttcaatacca
    12361 aagcagctca attgaacgga ccagagcaac tgcttgaatt tgaaaaagtc gtgatcttac
    12421 gtgtcgtgga ctctaaatgg acagatcata ttgatgcaat ggaccaatta cgtcagtcaa
    12481 tcggcttgcg tgcctatggt caaaacaatc cattagtcga ataccaaaca gaaggttata
    12541 agatgttcga agatatggta ggtgccattg aatatgaagt cactcgtatc ttcatgaaag
    12601 ctgagatccg tcaaaacgta caacgtgagc aagtttctca aggaacagct tctcactcag
    12661 aagatggaga tgccaaagaa aacagtaata ctagcgcgaa aaaacaacca atccatgtga
    12721 acaaggttgg tagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc
    12781 acggaaaaga tgcaaactaa tttctgacag caggattggt gagacaaatt cttttttaaa
    12841 gaatttgtct caccttcttt ctgttatttg acgattctgt tagactagat ataataaaaa
    12901 aagtatagtg aaagaaggaa aaaaacatgg aaatatccga aattcgaaat ctcctgacag
    12961 aaatgcagga aaaggtcaca agcttcagga ggtctctttg acttagacca gctggaagaa
    13021 gacattgcgg aagcagaagc aaaaatggct gaacctggtt tttgggatga tagcgaagct
    13081 gctcaaaaag tgatcagtga aaacaatgca aataaagaaa cttatgatca attccattct
    13141 cttgctgaag aactggaaga aatcgaattg atggtggaga tgttgcagga agaacctgat
    13201 gcagtaatgc aagaagaagc agaagcacgt atcaatgcgc tagatgaaaa aatgaatacg
    13261 tatgaattat ctatgttgtt agatggtcct tatgatcgaa acaacgccat cgtcgaattg
    13321 catccagggg ctggcggaac ggagtcccaa gactggggca gcatgctttt acgcatgtat
    13381 acacgctggg cagagcagca tgggtatcaa gtagagacac tagattatca agcaggcgat
    13441 gaagcaggaa tcaagagtgt gaccctactg atcaaaggac acaacgcatt tggctatctg
    13501 aaatcagaaa aaggtgttca tcgccttgta cgtatctcgc cttttgactc agcaaaacgt
    13561 cgtcatactt ctttttgttc ggtagatgtg atgccagagc ttgatgaaac gatcgatata
    13621 gaaatcaatc cagatgattt gaagatcgac acatatcggg cgagtggtgc aggtggtcag
    13681 cacatcaata agacagaatc cgctgtacga atcactcact tgcctacagg aacagtagta
    13741 gcgagtcagg cacaacgttc acaattgaaa aaccgggaac aagcgatggg gatgctgaaa
    13801 gctaagcttt accaattaga agtggaaaag aaagaacaag aagcagctgc actgagagga
    13861 gaacaaaaag aaatcggctg gggatcacag atccgttctt atgttttcca tccatattct
    13921 atggtaaaag accatcggac aaacgtagag acaggaaatg tccaagctgt tatggacgga
    13981 gatcttgatt tatttattga cggctatttg aaaatgcatc tgcaataaga gccctttgtt
    14041 aaattgaaat aaaattgtaa gattttgcaa gcaagttgaa acattgccat gctataatag
    14101 ctaaagactg aaaaagatat ggagagaacg ccatgattga aatgaaggat gtaatgaaga
    14161 agtactcaaa cggtaccact gctatccgca atctctcgat tgtcatcgaa caaggagagt
    14221 ttgtgtatgt tgttggtccg tctggagctg gtaaatctac attcatcaaa ttgatgtatc
    14281 gagaagaaaa agcttcaaaa ggaactttga acgtcgcagg tcatgatcta atagccatca
    14341 aaaaccgcga agttccttat ctacgcagag aaatcggcgt ggtgttccaa gactataaat
    14401 tattaccaag aaaaacagta tatgaaaatg ttgcgtacgc catgcaagtt atcggcagaa
    14461 aacctcggga tatcaaaaaa cgagtaatgg aagtgctgga tcttgttggt ttgaaacaca
    14521 aagtccgagt ctttcctagc gaattatctg gtggggaaca acaacgtgtt tctattgccc
    14581 gagcaatcgt aaatacacct aaagtactta tcgcggatga accgacaggg aatctagatc
    14641 cggaaaactc atgggaaatc atgaaactat tagatcggat caatgcacaa ggaacaactg
    14701 tcgtgatggc tacacataac agtacgatcg taaatacgat tcgacaccga gtaattgcta
    14761 tcgaaaacgg ccgcattatt cgagaccaag cggaagggga atatggatac gatgattaga
    14821 acctttttcc gccatttatt agaaagtatc aagagtttga aacgtaacgg ctggatgaca
    14881 gtagcttcgg tcagtgcggt aacgatcacg ttgactttag ttggtgtgtt catggcggtc
    14941 atcatgaacg caaccaaact agcacaagat atagaaggaa acgtggatgt atctgtgttc
    15001 gtagacattg gtacaaaaga cgaagagatg aaaaaattag aaaaagaatt aaatgactta
    15061 gatcatgtca aaaaagtcga attttcaagc aaagaacagg aattgaagaa aatccaaaac
    15121 gaaatgggcg attcttggaa attgtttgaa ggggataata acccgttata tgatgtatac
    15181 gtagtaagtg ctaccgatcc ttcttataca aaacaagtag cagaaaaagc ggcagatttg
    15241 aagaatgttt cccgttctga ttacggtggg gcttcctcag atcggatttt ccaaattgct
    15301 ggtgcagttc gaacatgggg attagttgcg gcggcattgc tgctgtttgt agcaatgttt
    15361 ctcatttcta acacgattcg gatcacgatt ttatctagac agcgtgaaat tcaaatcatg
    15421 cgtttagtag gggcaaaaaa tggatatatc cgctggccat ttttccttga aggtggttgg
    15481 atcggcttaa tcggtgccat tcttccgata ctgatcatta catttggtta cgcatggttt
    15541 taccaattga tcaatccgtc attattacga tctcattatt cattgatcca tccagaaaat
    15601 atcgtttgga aaatcaatct tttgatggtc ggtattggtg tgatcattgg ttcactcggc
    15661 tcgatcatct ctatgagacg tttcttgaaa ttctagtgtc acaagataaa tataatcaga
    15721 aagttggacg atttcgttcc agctttcttt ttttatcagc taggtggagt aaatggggcg
    15781 gcttttttct ctttacttta aaaaacaact tgggtattct taagttggta gaaaaagagg
    15841 tgaagccgtg aagattatcg ttgacaattt ttttacgatc cttttgatta tgaatatttt
    15901 gctttccttt attatcgttt ttcgagaaag aaaggaaact gcgcaaacat gggcatggct
    15961 gctcgttttg atgttcattc cagttgtagg atttatttta tatatttttt taggaagagg
    16021 gatttccaaa gagaaaatct ttgatttgaa gatccaaggg aaaatcggta agaatctaga
    16081 aatcgaagaa gataaacaag cattgatgcg agggctttat cctcatccgc ctactggaca
    16141 agtagatgtg aaacaattga tctatatgct tactgtattt gaaagtacgc tttatacgac
    16201 taaaaatgaa atcactttat ttacagatgg tcgtgaaaaa ttcgatgcgc tgatcgagga
    16261 tatcaaacag gcagaagacc atatccattt ccaatattat atttaccgca gtgatacatt
    16321 aggtgaagaa gttcgagagg cattgacaga ggcagcaaga cgcggagtaa aagtcagagt
    16381 ccttcttgat gcatggggtt ccacacaagt ttcttctagt ttttttcaga atttgaaaaa
    16441 agcaggtgga gaagtcgcat ttttctttcc tctattcgtt ccgtatatca atcctcgaat
    16501 caattatcgc aatcatcgta aaatcgtagt gattgatggg aaaatcggat atacaggtgg
    16561 gttcaatgtt ggaaacgaat atctaggatt ggtaaagaaa tttggttatt ggcgagacaa
    16621 tcatctgcgt atctatggag aagcggttta caccttgcaa aaccgttttt tgatggactg
    16681 gaattctcag catacaaaag aagtaggata ttcaccggaa tttttcccga ccattcattc
    16741 tacaggtgat atggctgttc aaatcgtgac aagcggacca gataccgaac atgagcaaat
    16801 caaaatgaca tatttgaaaa tgatttcgtt agccaaacgg gagatattga tccaaacgcc
    16861 gtattacata ccggatggat cgattcatga ggcattgaag ttggctcttc tctcaggcgt
    16921 aaaagtccat atccaaatac ctaacaaacc agatcatctt ctcgtttact gggcaacgta
    16981 ttctttcgct gcagagctga tcgagtatgg ggcaagaatc gaaacatatg aaaatggatt
    17041 tatccatgca aagacgatga ttatcgatgg cggcatcgtt tccgttggtt cagctaatat
    17101 cgacgttcgc tcatttagat tggattttga agtcaataca ctgatctatg atgaacgaat
    17161 ggcttctcgc gtaagaaaag cattctttga agattcaaag atttcgacgc atctcacaaa
    17221 agaagtgtat gaaaaccgag ggatgatgat caagatgaaa gaaggattag caagactgat
    17281 ttcgccgctc ctttaaaata caaaaaggac aggagagcca tcaatagatg gaaatctcgc
    17341 gtcctttttt ctcggggcca tttctgagag tacgtcagaa atggagaaag cgtctcgcat
    17401 gagcgaaatt aactttgggg tatctcttgt tttttgggga aagcaagaaa taagctaata
    17461 tatgctatta aaagagacac atttcaaagc cagtatagca tagaaacttt taatgagata
    17521 gctttttttg caattatgag aatttatttt gttatttaat gattttgatt cgttttcatc
    17581 aagtaatgaa aatgatagaa taaacatact ttaggattaa aggagatttc taatgaaaaa
    17641 actaaaatta ttcttaccaa taataggatt agctattctt ttatcaggct gttcaaagtg
    17701 gattgatcga ggagaatcca ttactgcagt tggctcttct gccttacagc ctttagtaga
    17761 aacggttgct gaaacctacc aggcagaaaa tcctggaaaa ttcatcaatg tacaaggggg
    17821 cggaagcgga actgggttgt ctcaagtaca gtcaggagca gtcgatatcg gaaattctga
    17881 tttgttcgcg gaagaaaaat cagggattga tgcttcggca ttagtcgatc atcgagtagc
    17941 agttgttgga atcactccta ttgtgaacaa ggatattggc gtaaaagata tcagtatgga
    18001 aaacctgaaa aaattattta cgggcgagat caaaaactgg agtgaagtcg gtgggaaaga
    18061 tcagccagta gttatattga accgtgcatc aggaagcggg acccgtagta cgtttgaaaa
    18121 atgggtattg gacggagaaa cagctgttca ggcacaagaa caagattcaa gcggaatggt
    18181 gcgccaaatc gttgcggaca cacccggagc aatcagctat gttgcttttt cttacgtttc
    18241 cgatgatgtg gatacgttga acgttaacgg tgtggaacca acagatgaaa atgtgacaac
    18301 gaacaaatgg acgatttggg catatgagca tatgtacaca agagaccatc caaaaaaact
    18361 gacaaaagaa tttttagact atatcttatc tgatgaagta caagataata ttgtcggaga
    18421 gctaggctat atccctgttt cacatatgaa agttgaacgt gattggcaag gaaacattgt
    18481 taaagagtaa tctttacaca aaatttacaa acttagctaa acttttacat gttattaaaa
    18541 ccaaattgat gttcaatttg tacactgagt gagtatgtta aacctgatta agggggagtc
    18601 atctgtggaa gatgtgaaaa aagtattatt aacaaaatca aaacgttcaa aaatggaaca
    18661 gcgcggaaaa ttcattagtt ttttgtgtat cgcgctgatc gtgttggttg tattatctat
    18721 tttttatttt gtcgctagta aaggattggc cacctttttt actgataaga tcaatgtatt
    18781 tgattttctg tttggtacag attggaatcc aagccaaata ggagcaaatg ggaaacccat
    18841 ggtaggggca ttgcctatga tcacaggttc atttattgtg acctttttat ctgctattgt
    18901 tgccacacca tttgctattg gagcagcagt gttcatgacg gagatttctc ctaaaaacgg
    18961 aacgaagatc ttgcaaccgg tcatcgagct acttgtaggt attccgtctg tcgtttacgg
    19021 atttatcggt ttatctgtta tcgtgccttt tgtcagaagt atctttggcg ggacagggtt
    19081 cggtatctta gctggtacat ttgtgttatt cgttatgatc ttaccgactg tcacaacgat
    19141 gactgtggat gcattaaagg cagtgccgcg acactatcgg gaagcttcat tagcgctagg
    19201 tgccacaaga tggcaaacaa tctacaaagt ggtgctgcga gcagcagtgc ccggaatttt
    19261 aacagcagtc gtttttggta tggctcgtgc gtttggcgaa gccttagcta tccaaatggt
    19321 tattggtaat gcggcattaa tgccaacagg acttactact cctgcttcta cattgacgtc
    19381 catcttgaca atgggaatcg gaaatacaat catgggaaca gttgaaaata atgtgttatg
    19441 gtcattagcg ctgatcttgc tattgatgtc attattcttt aatatcgtga tccgtatgat
    19501 tgggaagaaa ggagagctga aataatgaac gcaaaacgct cagataaaat tgctacaggt
    19561 gttctatatg tggtttcagg gatcatcgta ttgatcttag cagctttatt actttatatc
    19621 ttagttcgag gaataccgca catttcatgg tcattcttga ctgaaccttc caaagcctac
    19681 caagaaggcg gtgggatcgg gattcaatta tttaactcga tttatttgct tttgattacc
    19741 atgatcatca gttttcctat ctctttaggt gcgggtatct acctttcgga atatgccgga
    19801 aaaaactggc tgacagacgt gatccgtacg tctatcgaaa tcttgagctc tttgccttct
    19861 gttgttgtcg gtttattcgg tttcttggtt ttcgttattc aattccagta tggtttctca
    19921 atcatttctg gtgcattagc tttgaccgta ttcaacttgc ctcttttgac tcgaacggtc
    19981 gaggaatctt tacaagcggt ccattacaca caacgagaag ctggattggc gttaggtttg
    20041 tcacgctggg aaacagtgat aaaagtggtt gttccagaag cgacaccagg aattttgact
    20101 ggtgtgatcc taagttcagg aagaattttt ggtgaagctg ccgcattgat ttatacagct
    20161 ggtcaaagcg cgccagcatt agattttacg aactggaatc cattaagtgt ttctagtccg
    20221 atcagcattt tccgccaagc tgaaacactg gctgtccata tttggaaaat caacacagaa
    20281 ggtacgatgc ctgatggtgt ttctgtttcg gctggcgctt ctgccgtgtt gatcattgct
    20341 gttttactgt tcaacttcgg cgctcgtgcg attggcaaac gattataccg aaaaatgact
    20401 tcagcctaag gagaagaagt aaaatgaaaa aatataattg gaatgaaaca aatgtcatta
    20461 ccatgccaaa agataaggaa gttgcattat atacgaatga tctgcatgtt tggtatggcg
    20521 acaatgaagc aatcaaggga gtggatctag aattcgaaaa aaataagatc actgcattga
    20581 ttggtccctc tggctgcgga aaatccacgt atcttcgttc tttgaatcgt atgaatgatg
    20641 ggattgctaa tacgaaaatt actggaaaga ttatgtataa agacgtggat atcaatgcgc
    20701 ctcaagtgga tgtttatgaa atgcgtaaac ggatcggcat ggtgttccaa cgccccaacc
    20761 cttttagcaa atcgatttat gacaatataa cttttgcttt gaaacagcat ggtgaaaaaa
    20821 acaaaaagaa attagatgaa atcgttgaaa caagcttgaa acaagcagct ttgtgggatc
    20881 aagtaaaaga tagcttggat aaaagtgcct tggctttatc tggcggacaa cagcaacgtt
    20941 tgtgtatcgc tcgtgcaatc gcgatgaaac ccgatatctt gctgttagac gagccggcaa
    21001 gtgccttgga tcctatttcg acaggaacgg ttgaagaaac acttgtaaac ttgaaagaag
    21061 actacacgat cattatcgtg acgcataata tgcaacaagc agctcgtatc agcgactata
    21121 ctgccttttt ctatctagga aaagtaatgg aatatgacaa aacaaacaag atatttacca
    21181 gaccaaaaat tcaggcaacc gaagattatg tgtccggtca ttttggttaa gacggaggcc
    21241 tcaacatgac aaaagaaatt atctcatcca aagatcttca tctgtattat ggaaagaaag
    21301 aagcactaaa gggaattgat ctaagtttca atcaaggtga aatcacagcg atgatcggtc
    21361 cttctggttg cgggaaatct acctatctac gttcgttgaa tcgaatgaac gatctgattc
    21421 caaatgtaac gatcaccgga agtgttttat ataaagacaa agacatctat ggtccgaaaa
    21481 cagatgttgt tgaactaaga aaagaaatcg gcatggtgtt ccaacagcca aatccatttc
    21541 ctttttctat ttatgaaaat gtcatctatg gattgaagtt aaaaggtgaa aaagataaaa
    21601 aagtccttga tcaagtagtg gaagaaagtc tgaaggcagc gtccgtttgg gaggatgtga
    21661 aagataaact gcataaaagt gccttgtctt tatccggtgg gcagcagcag cgtgtatgta
    21721 tcgcacgtgt tttggcagtg aatcccgaaa tcatcttatt ggacgaacca acaagtgcac
    21781 ttgaccctgt ttctactggg aaaatcgaaa gtatgctgtt agaattaaga gagcggtata
    21841 cgatgatcat cgtgacccat aatatgtctc aagcttcacg tatttcagat aagacggcat
    21901 tctttttaga tggtcacttg atcgaataca acgatacgaa gaagatattc atgaatccag
    21961 aaaaacaaga gacagaagat tatatttcag gtcgatttgg ataaggagga aacggaatgc
    22021 tacgtacaca atttgaagaa gaattgctga atctccataa tcagttctac gaaatgggga
    22081 tgatggtgag cagcgctgtg cacaagtcag ttcgtgcata catcaaacac gataaaatct
    22141 tagctcaaga agttatcgaa aacgatataa atatcaataa tatggaaacg cgtttggaga
    22201 agaaaagttt tgaaatgatt gcattacaac agccagttac aactgacttg cgtatgatta
    22261 tcacggtcat gaaagctagc tctgatttag aacgtatggc cgatcatgcg gtgtccgttg
    22321 ctaaatccac aatccgcgta aaaggacaga cgagaatacc ggaaatcgaa aaagagatct
    22381 ccgatatgtc cgattacgtg aaaaaaatgg tggacaacgt cctagttgcg tatgtcaaaa
    22441 cagacgaaaa agatgcgcgt acaatcgcca atatggatca aagagtcaat gactatttca
    22501 atcgaatcta taatgcaacc atcaaaaaca tgcaagaaaa tcctgaaaca gtcatcagtg
    22561 gcacagacta tttgaatgtt gccagctatc tagaacgaat cggagactat gtgacaaaca
    22621 tctgtgaatg gatcgtctac ttggctacag ggaaaatcac tgaattaaat acgaaccaca
    22681 atgagatttt ctaaactgaa gttgtgattc tgctgtttat ttaggaaaag aggttgtgaa
    22741 atcagcgttt tgtcctgagt attagagaaa caaacggtaa aatagttcct catattttgc
    22801 cgttttttga gctaataccg aaggtcagct gattgaacac cgtttattct ggaaaagggt
    22861 ttgtgacaaa acatgtcaca aacccttttc attattccaa tagaaaggat aaagaatgca
    22921 aaaaaagatt agcatcatca tcactctatt ttttatttgt atacttgctt catggtttat
    22981 tcagcgtcaa gctgctctat atgacgtacc tgtcatcgag atcgaaagag tgagaataga
    23041 agaggatcaa caattgatta caggaagatt gatgaatacg gaccaaaaag gagaagtgat
    23101 ttctttaacg gctccttatc aaaaaaatca aatcgaaaca gcaaatataa caacaagaca
    23161 aatctatctt gtccaaatgc atggagatga atggcaacta gtagcaaaaa aaaatgatgg
    23221 ctggcttttt ttctttacaa gtgtcttcat tggcaccatg ttgcttattg gtggaaaaca
    23281 gggcgtattt gctttaggaa gtctcatcat caacatcgtt ttacttttac ttttgttgct
    23341 cctctttacc aaaacagaag gtatttctct cttaaatata gcaatcttat ttgtcgttat
    23401 agggataatc gttagtatat tggcgttaga cggatggaat agaagcagta taatcaaaat
    23461 ttcggctgca gttacaagtg tttttttagc attttttatt tgtcttttga caatgaatca
    23521 ttggaaagat caaggactgc gctatgaaga actgaattat ctgacacgac cgtatcgttc
    23581 tgtttttctt tccagcgtgc tgattgggac agctggaggg acactcgata ccgtgatcac
    23641 agtcattgcg acattagaag aattgaccaa gcaacaaccg aaaatatcag cgaaacagtt
    23701 atggctttca ggaaggcaag tgggccgaga tgtgattggt tcgatggcta atatcctttt
    23761 attcgtctat atcagcggag cgattccatc gcttctgtta tatttaggaa atcaatggtc
    23821 tttcaaagaa acggtagaac gacatttatc attagaactt ttacgcgtca ttgctggcag
    23881 cttaggaata gtattatcaa tccccatcgc ggtcctcttt tttctcgctg taaggaggtt
    23941 aaaaaaatga atgtacttat tatacttgtt gggatttttg caatcagtgt gctatttgtc
    24001 ggtggtacgc aaggtatgta tattctttta ggattattca ttaatttagg aatctttttc
    24061 ctattattat tcggctatca tcagaagtgg ccgatattgg tattgtcgat tatagggttc
    24121 ctattgatcg ctgtggttat cttatttttt atcaatggtt ataatctgaa aatgcgagcc
    24181 gcttttgcca gtatactgat ctttttgttt tgtttcctac tattgattcc tatcactgat
    24241 tttttagcga tccaaggctt tacttcgata gaactagaag aactatctgg cttagataag
    24301 acgttagcaa ttgattttag actacttacg aggtcgttgc ttctgatcag tcttagcgga
    24361 gctgttttag atgcgagtgt agcgatttct agtggaacat tcgaagtata tcaggcaaat
    24421 ccgcaaatgt cttttaacca attgcgccat gctagctttg ccattgctaa aaaaatattg
    24481 gcatcaacag tgatgacgct tctttttgcg tttatgggga gttctttagc gttgattctt
    24541 tggtttattg acttagacat ttcttttcag caaataatca atgaaaaatc atttgtcttg
    24601 gaatatacga tggctattct taccacactg gccgcactac ttgtcctgcc cttaacttgc
    24661 gtgactacag catatctctt tacaaaaaag aaagaacatc tcaagatgga gtaaaagtta
    24721 caacttaagt cctatgacat ttttactgaa tcagtgcata atatggatgt aaggaaaagc
    24781 ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag aaagagaacg tattttagac
    24841 ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag atttattaga aagcatggca
    24901 actgaaaaag acgaaaaaca aatcaaaaaa gaagcagaca aggtaactgc tagtcataaa
    24961 gaaaaagatc aggcaagtca attgatcgat gaacttgaaa acggtgaaga aaaaatttct
    25021 gagccagttg atccaaaaga aaaagaaaga caagatcaag aaaacttaga aaagatctta
    25081 gatgaactag caactgaagc gaataaaact tcggcgcgtc tagacgaagt aaatgctcaa
    25141 ttcgctgaca ataaggcagc acgtaatgag aaacaggaag cattgatgca gctgaataca
    25201 aaagaagaat taggcgagtt gacagaagaa gaattagcac agcgtcaaac gttagaagca
    25261 gaaatcaaag agttagaagc aaccggcgat acactattag aagaacaatt gaagctggaa
    25321 gcagaattaa aagatatccg caagaatcaa tggtctgaga aaaaagaaac atttacggat
    25381 aagtttgaat tgccagatga ttggaaagat caagcgacag atacgctgaa tcaagtaggc
    25441 gaaaaaatgt cagaagctgg atcacagtta ggcaaattct tgaagaaaac attccaaact
    25501 gtttcagaaa cagtaaatga taacatggaa tggaaagatg tcagcttacg cgtccctgga
    25561 attgcgacaa cgaaatttga acacgaattt tattatgaag ctcctgcagc aagtatcctt
    25621 gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt gggacagtga tgatgtgaaa
    25681 gtggaagcca agatcaaact ttacggaaaa atgggtgctg aaccatttga agcattctct
    25741 gaacggagcc agatcgaggt caatgaagat catatctcct tccaaattcc aaataagcgt
    25801 gtgcgtgcag accttgtttt ctacttgcct aaacgagtat acgaccatgc agctatcaaa
    25861 cttctaaatg gcaacatcat gattgagaca ttggaagcta aagatattta tacaaaatct
    25921 acgaatggga acatcatcgt gaaccaatta actgcaacga tgctggaagt agaaggtgta
    25981 aacggcaata tcgatattcg aaacgggaac atcttagatt ctattataga aacagtaaac
    26041 ggtacagtga catttggtgc cacaccagaa aacctttctg tgtctttagt aaatggtgat
    26101 gtacgtctta cgattaaaga agataatttg aaaaaagtag aagcaagttc agtgaacggg
    26161 aatgtcaaag ttgctttacc agatagcatc ggtttggaag gacacgctaa aacaagtctt
    26221 ggaagcatta acagccgtct gtccaattac gaagtagttc gtgagaaaaa agaacggaca
    26281 aaccaaatgt tgcaattccg tcgtgtttca gatgatgaga tcgcacaagt gcagctttca
    26341 acgacaacag gaagtatcta tttaaaagat acagataaat aaagaggttg tgaaagagat
    26401 gttcagcctt gagcaataag aagcgatttt ttaaaatagc ttcccctatt ttaaaaaaat
    26461 gtgccttatt gccgataggc tatctcttga acaccgatta agaagaaggt tgtcacaacg
    26521 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
    26581 atccaataaa aatgttgttt tgacaggtac tttagcaggg attggggaat atatcgggat
    26641 tgatcccact gttatccgtg tgctttatgt attcttaagc ttagttgctt ttggaagtcc
    26701 aatcatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
    26761 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa caaaaaaacg
    26821 caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcag
    26881 cgtattttgg ttaatacgtt aacattcata tcgttggctg ttctttttcc tgcgatggtc
    26941 catgtgagca gtatttggac ggccgtcctt gccagcttcg tattatctgt gttgaacatg
    27001 tttgtcaaac cgattttgac gatcctgtcc ctgcccttta cattagtcac attgggattg
    27061 tttacgtttg ttatcaatgc agcgattctt aaaatgactt ccttttttgt aggagcagaa
    27121 cattttagtt tttcttcatt tggtgcagcg ttattcgtag ctatcttgat gtcacttgtc
    27181 aatgtaattg ttagtgaaca taacagcaga aaataaaaaa agctgtgccg agatctcggc
    27241 gcagcttttt tgtttagaat aaagaatgcg ttttctatta cattggtaat taaataaaaa
    27301 cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag agaacactct
    27361 aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt agtaaaaaaa
    27421 ttatctttgg aaatcgtcac aggcgacgag aaaagtttag accgcgtcat cgtgactggg
    27481 gacatctcac gcccaggact agagttgacc ggttatttta attattattc ccataatcgt
    27541 ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc agaagagcgt
    27601 ttgatcatta tgagaagatt atgtagtgaa gatatgcccg catttattat ttcaagagga
    27661 ttgccggctc cggaagaaat gatccaagca gccaatgaaa atgggatagc tgtgctgcgt
    27721 tcgcctatct caacgtcccg gctgctaggt gaattatcta gttatttaga tggacgctta
    27781 gcccctcgaa caagtgtcca cggtgtgtta gtcgatgtct acggattagg tgtactaatt
    27841 caaggggaca gtgggatagg gaaaagtgaa acggcgttgg aactgattaa gcgcggacat
    27901 cgattgatcg cagacgatcg agtagatgtc tatcagcaag atgaactgac ggtaatcgga
    27961 gaacctccta aaatcctgga acatctgatt gagattcgag ggatcggtat catagatgta
    28021 atgaatcttt tcggtgcgag tgcggtacga ggctctatgc aagtccagtt agctgtttat
    28081 ctagaagcat gggcgaaaga taagaaatac gaccgtctgg gctctgaaga cacgaccgtt
    28141 gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg acgaaacgtt
    28201 gcaatcatca ttgaagtagc cgcaatgaat ttccgcgcac gtacaatggg ctttgacgca
    28261 acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg caatgactag
    28321 caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt gtaggaattc
    28381 ctatctactg gtatgcattg attatcgtat caggaatcat tattgccatg tggctcagca
    28441 gcagagaagc tgttcgggta ggattaaaag cagatgacgt gacagatttt atgttgtggg
    28501 gattgccgat ttccattatt ggtgcccgct tgtattatgt tttattcgat cttccccaat
    28561 atatagctga tccaatccag atattcaata tccgttcagg aggattagcg atttacgggg
    28621 gattgattgc aggtgcaatc acactgtact tttttaccaa atatcatttt atttctttgt
    28681 ggaccttttt agatatcgct gcaccaagcg tattattggc gcaggcaatc ggacgatggg
    28741 gaaatttcat gaaccacgaa gcgtatggtc cagacacgac acgaagtttc ttagaaagtc
    28801 tgcatttacc gcaattcatc attgataaca tgtatatcga aggaatctat cgacaaccaa
    28861 cctttcttta tgaatcactt tggagtttga ttggatttat tctgcttttg gttttgcgga
    28921 agaaaccaca tcttttgaaa aaaggagaag tccttttatg ttatctaatg tggtatagtt
    28981 tcggacgttt ctttatcgaa gggttacgca cagacagcct ttacttttta ggtgtgatcc
    29041 gcatttctca attattgtct gctgttttat ttgtcggcac attgattctg tttatttggc
    29101 gtagaaaaaa aggaagttta ccggattatg accgctcatt aggaaaaaat caacaaatta
    29161 tttgattttc agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat gaattataga
    29221 gtagcggttt taggaccagg ctcttggggg actgctttag cacaagtcct cgctgaaaac
    29281 ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat caacacccaa
    29341 cacaccaatc aacgttattt accagatcat catctacccg attccatccg agcattcacc
    29401 gatatggaag atgcagtaaa agatgcagat gcggtactat tcgttatccc gacgaaagcg
    29461 atacgttctg ttgcaaaaga actagttgcc aaattgaata cgaagccagt gatcatccac
    29521 gcaagtaagg gattagaaca gggaacgcat aaaagaatct ctgaaatctt gttagaggaa
    29581 atcccgacag aaaaaagaaa agctgttgct gttctttctg ggccaagcca tgcggaagaa
    29641 gtagcaaagc atgacattac gacgatcaca gcggcaagca caaatgtaaa agcagcaaaa
    29701 tatgtacaag agctgttcat gaaccattat tttcgaatct ataccaaccc tgatgtgatt
    29761 ggtgtagaga tgggagcagc tttgaaaaat attattgcca ttggagctgg tgctattcac
    29821 ggcttagggt ttggcgataa tgcaaaagcg gcaatcatga cgcgcggatt agctgagatc
    29881 agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttattggctt aagcggagta
    29941 ggtgatctag tcgttacttg cacaagtgtt cattccagaa actggcgcgc aggcaacttg
    30001 ttaggacaag gtcagcctct agaagaggtg ctagccaata tgggaatggt tgttgaaggt
    30061 gtgaatacta ctcaagcagc gatggaacta gcccaaatga tggaaatcga aatgcccatc
    30121 actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa
    30181 atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt ttgaaggagc
    30241 gaatgtatat gaaagtaaaa aaagcagtta ttccagcagc aggattagga acaagatttt
    30301 taccagcaac aaaggcgatg gcaaaggaaa tgttgccaat cgtggacaaa ccaacgatcc
    30361 aattcatcgt tgaagaagcg ttgaaatcag gaatcgaaga catcttgatc gttacaggaa
    30421 aagctaaacg accaatcgaa gatcactttg attcaaatat cgaattagaa acaaacctaa
    30481 gtgaaaaagg aaaaaccgaa ttattgaaat tagtagaaga aacgacagat gtcaatttgc
    30541 atttcatccg tcaatctcat ccaaaggggt taggacacgc cgttcttcaa gcaagagcct
    30601 ttattggaaa tgaaccgttt gtcgttatgc taggcgatga tatcatggaa gacgaagtac
    30661 cattgacaca gcaattgatc gatgactatg agcgtacaca cgcttctacg attgccgtta
    30721 tgcaagttcc tcacgatgaa acttcaaaat acggaatcat caacccaggg gaagtactgg
    30781 aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa
    30841 gtgatttagc tattattggg cgttacttat tgactccaga aatctttgat gtattatcag
    30901 aacaacaacc aggcgctgga aatgagatcc aactaactga cgcaatcgat acgttgaata
    30961 aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttatgacgtt ggtgacaaat
    31021 ttggttttat gaaaacaagt attgaatatg gtctgacaca tccagaagta ggggcacctt
    31081 tgcgtgaata catcatgtct ttaggtaaaa cattagaagc agaaggaagc aagatcaatc
    31141 ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataatct ttttttctaa
    31201 cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt tgtttaaaaa
    31261 acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat catgtcagga
    31321 ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt tttcatcgtt
    31381 cgcgtattgt tgcaagtttc caaaacttta gaaaaagtag accagactgt cgcagaagca
    31441 aatacgacaa ttgagatcgt cacacaagac gtagatcttt tatctagaca agtagaaggc
    31501 ttgctagtca aaagtaatga gctgctcttg gatatcaatg gaaaagtcgc aacgatcgat
    31561 ccattgttca cagcagttgc tgatttaagt gaaagtgtat cggaacttaa tcattctagt
    31621 aaaaacatcg tgacgaaagt cggcggaatc ggaaaaacga cagctaaagc gactgttgcc
    31681 ggaaaagtcg gtggaacagc gatgaaattt ttaaaaacaa aaaacatact gaaacgacag
    31741 gaggaaaata agaatggcta aaaaaggtgg atttttatta ggtgcagtag ttggaggtac
    31801 agccgcagca atcgccgcat tattgttagc acccaaatct ggtaaagaac ttcgggatga
    31861 attggctgct caagcagatg atttgatgga catggcttca gattacacag atatggcaaa
    31921 agaaaaatca tctgagttaa caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca
    31981 agcaggggat ctagcaggca atgttaagca gaaggtaagt gaatcagcag aggaagcagg
    32041 aagcgaatca gatgatatct tgttaaacct caaagaacaa tcagctgatt tatctaatcg
    32101 tttcaaaaaa acagcagaag atctgaaaaa gccagcagac gaattaggta gtattgctaa
    32161 agatacttca gaagatatca tgatcgatgt aaaagatgct gcagataaag caggagagac
    32221 ggtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gacacaaaag aaatgttgaa
    32281 aatggcaaaa gaagatgcaa aagaagatgc aaaagaagct aaaaaagaaa tgaaagatac
    32341 aatgacagat tcgactacca atcactaaga ttactcattt ctagaataaa aaaggtgaga
    32401 gcggaccagc tctcaccttt tttattttca ttactctaaa acaagtaatt cttttggtgt
    32461 agtcgtgaat ggcacacaac catcacttgt cacatacaca caatcttcga tacgtacccc
    32521 tactttttca ggaatataga ttcctggttc aagagagaag cacatccctt cttcaatcac
    32581 taagtcgttt ccttctacaa gagaagggaa ttcgtggacg gttgttccga ttccgtgacc
    32641 taaacggtga ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg
    32701 atcaagttca cctgctgtca cacctgggcg aactgcctct tgtgcggcaa gctgtgcttc
    32761 aagagtgatg ttatagattt tttcttggaa ttcagttggt ttttgataag caactgtacg
    32821 agtagcatca ctgcaatagc cattccaaat aacgccaaga tcgaataaca ctagttcatt
    32881 tggtgaaacc ttcgtgcttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag
    32941 gactaatgtg tcgaaagaca tttgtgaaac accttgtttt tttaattggt attcgatttc
    33001 tgcgataatt gcttgttcag ttacgccttc ttttactgtt ttgaagccaa tttcaaaggc
    33061 aacatcggcc catttaccag cttcgatcag tttgtctttt tcttctaccg ttttgatcag
    33121 ttgcagacgc tggataatag aagttagatc ggtgtaagaa tctgtacctg tcaagatatc
    33181 agagatagca tcgaagcgat cgacagtcag agcattcttt tcaataccta cattaccagg
    33241 ttgcccatag cgtgcgcgaa gctcttttcc gatgatctcc caaggatttt cgctgtctaa
    33301 gtagccacgt acatcgtacg cccagctgct gttttgagca tcttcaactt ccagagcagg
    33361 agtgaaaagg aaaggatctt gttccagagg gataaaaaga gctagtacac gctcgtgagg
    33421 atcactttca tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc
    33481 taattggtgt tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca
    33541 acctactttc taaattaatt ttgtattcaa taattgtacc actgattgat taaaagacca
    33601 acgttcttaa aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa
    33661 ttgttgagat tatttgatat cctattttag aaatgtaaga cgtattttca gaaaacatat
    33721 aaaatatgaa taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga
    33781 gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca
    33841 aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaatg
    33901 cagtcgcacg tggcttggca agtaagaaaa caacaacagt tggggtaatc attcctgatg
    33961 tcagcaatat gttctttgcc tctttagcac gtggtatcga cgatgtggca acaatgtaca
    34021 aatacaatat catcttagca aactcagatg gcaacgatca aaaagaagtc aatgtattga
    34081 ataatttatt ggctaagcaa gtggacggcg tgatctttat gggtcaccat atcacagacg
    34141 aaatccgtgg agaattttca cgttcaaaaa cgcctgttgt actagctggt tccattgatc
    34201 cagatgaaca agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa
    34261 ataaattagc aaaaagcggc aataaaaaaa tcgcttttgt cagcggtgcg ctgattgatc
    34321 ctatcaatgg tcaaaaccgt ctaaaaggct ataaagaagc attaagcgaa aacaatctac
    34381 catatagcga aggattgatt tttgaggcac aatacaactt taaagatggt ctgtctctag
    34441 ttgaccggat ccataatagc ggtgcaacag ctgtctatgt ttcagacgat gaattagcca
    34501 ttggtatctt ggatggttta ttagaccgcg gcgtaaaagt accagaagaa ttcgaaatca
    34561 tcacaagcaa caattcttta ctgacagaag tagctcgtcc tcgtttgaca agtatcacac
    34621 aaccattata cgatttagga gcggtatcca tgcgcctatt gacgaaaatg atgaataaag
    34681 aagaagtcga agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatcaacta
    34741 aataataaaa gatcaatcat ttaaaggttg ttcccaaagt ggtcaacttc tggaacaacc
    34801 tttatacgag aaaaggggtt atgacaaaat tttgtcataa cccctttttt tgtgacttta
    34861 aaaaaaagag agaatggcgg aaacaaacct tacgtccatc gtatacgaca caatctgtgc
    34921 gtagatgcta cattttgttc actactatca aaattacttt aaaatgaaaa cgtgataaaa
    34981 aagaaagtag aaaagtagaa aagtaactct aaaaaatcca aaaaaatcca ggctcgattg
    35041 cgtacttatc ttaaaggaga aacaatccat catacataac ccgaaaggag acttctattt
    35101 gcctaagaaa agacgaaaga ccatagtcat cattttgacc attcttacta taggaatcgg
    35161 aggaaaattt tacatggaca aaagagaagc gcaaaaacag caagatttac tcgcagtaga
    35221 aaaacagtcc gtcaaagttc tgaaaaatac ctttgcggat atcaaagaag tgaaaatcga
    35281 acaatttgag aaaaacgaca tgacaggaat ctatgaagca ttgataacga tgaccaatac
    35341 agaaggaaaa gccgtttatt ttgattatag ctttacaaaa gaaagcaatg agatagatgg
    35401 ctatggtgtt gaaaatgaag aagtacaaaa agaagggatt acggttgatg agattatagt
    35461 tatctataca gatgggagtg aggaaaaagt atgacaatta ctgataagca gtataaagga
    35521 ataagtaata aagtatattg gctagatcca aaacatcctc aatatagtaa aagttacaaa
    35581 gaaggcgcca ttaaaaactt tggagaaaca aaattccaaa tcctaaaaat caaaaactcc
    35641 ttcgacggca tgcaggcgat ggctgtagcg ccaatcaaga acggtaaagt ggatatttct
    35701 caagtagtga ttgcttttgc aggaacgaac ttaagcgatt ggaaagattt ggaaacggat
    35761 gcaagaagtg ttatgaatct ctatgggaat agaccaggag caagtttttc tgaaaaagca
    35821 acaacaaaca agattttatc cgttagtgga ttgaaaacag cggggcaaat caattcagcg
    35881 attgcatttg ctaatgaagt aaaaaaggaa tatggagaag ctgctatttc tactaccggc
    35941 cattcattag gtggattctt agcattatat gtagcagcag agaaacaatg gaaaaatgtc
    36001 ggcttcaacg ggccggaccc gtatgatctc ttatcaccag aagcaaaaaa atgggtggaa
    36061 gaaaatcccg gtatgctcac caattaccga aatcacgccg attttatcgg taattttggt
    36121 ggtaatggca caggtgctga agttcggatc agtatggaaa cagcatactt gaatctgctt
    36181 gcttcccaca gtttaacctc ttggaagttt gatgaaaatg gaaagctgat tattcctgaa
    36241 aatgaaaaca atgttaaagc tcgaaaacaa cagaaagaaa accaagcgac ccagttgttt
    36301 tcctatcagt tgcaagaact gaacaacata cgtaaaaaac tacgattaag tggcggccgg
    36361 ctgacagtta atgaacaaat ttacttagat gacacacaag ctaggttagt ggtggaaaag
    36421 ttatcggtaa aaatgaaaag cgctatggaa gaagttatcc gcatcaacca aagaggaatt
    36481 tttgagctag agaaaaaatg gcaagaaggc ttgcggcaag cacgtgttgt cgccccttcg
    36541 ttgactgaac aggaaatcat agaagcatta gctagtgtgg gcgccacaaa agagaactta
    36601 gtcgattctg cgaaagaagt ctatctgcta cgaatcaata aggcaagacg tatgggggag
    36661 gcctttgata cattggcgaa ggagatccaa gacaaaatca atgaaatcgt ggcacgagat
    36721 cgagaattag cacagcaatt caattaaagg agaagtagat gaaactagaa gaattggaaa
    36781 gagattatcg aagtgaacga agacaattag aagctcaaga agatgaattg atccacttta
    36841 agaatcaagg attacgattg atggagcaag aggagcagcg actgcatcat gtattaaaga
    36901 acttagcttt agataatgag cctctgtttc aagcaagacg aagttattcg aattatcagg
    36961 agcattatca acaagcccta caagtagaac gacgaaaact ctatcagcag caagacgaac
    37021 tagaacgttc ctatcgtcaa aaaatcagac aaataactat ggagaaatca tcttatgaat
    37081 gaatttttaa tgcaattact aaaagaatta gagaaaaatc tacatcgatc aaatgtacgt
    37141 tcagttggta tggggatatc tcagcaaact tgggcagaaa aacgattgac agatcaaaac
    37201 caccaaatga ctgcagaaga agctactcaa gaattttcta accgacatgc acacttaaaa
    37261 gaatcagtaa aagggcaatt tgcagaaaaa ctaaccgaat tattccaaag acaagaaaag
    37321 aaattaaaac agtttcgtta gtaaagttat atagaaaaat tttgtgaaaa tggtttttca
    37381 catttttaca taaatttgtc ttaatatcgt agatgtaagc ttttgaacac catttattcg
    37441 gaaaaaggga gctgtgacaa gatttttgtc gcagctccct ttaattctga ttaatctatt
    37501 agatcaatca tctttatcgt ctgattcttt ggtctctgga ttcgctctag cataagctga
    37561 agcggtgttc cagtaatctg tatagttttc atcagtaccg ccaacgccga atttaaaggc
    37621 gcttttcttc ggaccatttt ttgcccaaag tgattccact gtttttcctg gcgtttggat
    37681 agatttttta ttgacagtaa cttttccagc tgtttgtcct gtgaacccag atactttttt
    37741 cttcatcaca tcggatgata agtcaaattt ctcatcagtg ccgaaaatct cagggtttgc
    37801 ttggtaagct tgattgatta gattggcaag ataattagcc gaacgtattc ttgcttggct
    37861 gtccattcca gtattgtcat cgtgtcctgc ccaactactg atagtgatag atggagttga
    37921 gacgatcagc catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc
    37981 atttcctaga tttccgttta agcttgagat gacatctttg aatggtgtag tgattttttc
    38041 attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttcgg aataaatacg
    38101 gacagaatta ctttcatgtt tataaatcac attacctgca ttatctgtga tggaatcaat
    38161 gatatagcct tcttcgtaga ctccaccatt tgctaaggcc tggaatccat ttgtctgttg
    38221 aagcgtcgtt acatctactg tccctaaagg agccgattca acgccccaat tatcatttgc
    38281 tgggtagttc atcttcgcca agtagtgctc gtaagcgtat tgtttcgatc caccggaatt
    38341 caacacatct tgatataagt gataagctgg gatattgttg gaccattcca gtgactcacg
    38401 gacggtttgg aaggtgttcg acccttcatt ggtcgcattg acgattttct caccagcatt
    38461 ttttccttct tgccaagtcg ttgcataatc tgatacacgg gattctgatc cgacaagtcc
    38521 catatcgatt gcaggaccgt aaacaagaac cggtttgatg gatgaaccag cttgacgttc
    38581 tgtatcaaag gcgtggttat tttgattctg gctgtaattc cgaccaccca cgaagccata
    38641 gatacgtccg gttctgttat ccatcaatac atttccagtc tccacttggc taccagcacc
    38701 atcatctaac atgtagccat aattttctac gccggtttgc aatgcagcat agatatcttt
    38761 atcaatcgtc gaatggatcg tatatccttt gttttggatc gtttgctgtg ctttttgata
    38821 ataagcttca ctgaccgatg aatctgacat atcagcatta tctttttctg ccaattgttg
    38881 tgcaataatc cttgttgctt cgttcatcac ggtataatac aagaattctc tgtcagtctg
    38941 ttccgcgatt tgttgtggga gaaagtcttt tgtaagatca taggctttcg cttcttcgta
    39001 ttgttctttc gtaatttgtc cttcacggta catactgaat aaaacaaaat cttttctggc
    39061 aagaccggca gaaagatctt cttttagcgc gcctgtattc gtgtatggac tatacgtgat
    39121 agggctttga ggcaaaccag cgatataagc agcttgcggc agagtcacat cctttgcatc
    39181 tacaccaaag attcctctag cagcttcctg gactccagca atattttgcc ccttattatt
    39241 ccgaccaaat ggtgagacat tcaaatacgt agtaatgatc tcatcttttg aaaagtgttt
    39301 ttctacttgt gcagaaagaa cgatctcgtt tgctttacgt ttgaatgttg tttcatcggt
    39361 taggatctgt tgtttgacta attgctgtgt caaagtggat cctccagacg agccgatgcc
    39421 agtcgcttct gaaactagtg ctcgtaaaac agctttaggg acataacctt ggtgtttatc
    39481 gaagtattca tcttctgtac tgataatcgc ggttttcaat aaaggcgaaa tcttatcaga
    39541 tgaaatagtc gtccgcatca gatctgtctg gatttttgat atctctgtat tatctgcata
    39601 aacaatcttt gacgtttccg taatgtttcc tagttctgtc tgcagttctt ttttagtcgg
    39661 caattgtgtg tcttcgacaa gataggcaaa gtagccagca cctattccga tacctagagc
    39721 tgcgaacaac aaaagaagaa cagtaataaa aacagtcaat gattgaaaca ctctaaaaat
    39781 aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt
    39841 gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag
    39901 gacaaaaaaa cagacaaggc tgtttttgac aggaatttct ttttcgaatg agataaaacg
    39961 tgaggaaatg atctttttta tgaaagagtt cttaagcttt tctttaggga aagtttcagt
    40021 tactatggag aacagggcaa agaaaaacaa actattttct ttgtacatca tcttatcaat
    40081 atacaggaat tgttcgaaac aattgccttt caaggaggaa tccatccatg ttattaggct
    40141 cacacgtcag catgagcgga aagaaaatgc tgttaggcgc ggcagaagaa gcagccagtt
    40201 acggatcttc gacctttatg atctatacag gcgctcctca aaatactaga agaaaagcta
    40261 tcgaagagat gaatattgat gccggaaaag catttatggc agaaaacgga ctaacaaata
    40321 tcgtcgttca tgcgccttat atcattaatt tgggaaatac gatcaaacca gaaatgtttc
    40381 catttgctgt acaatttctg agagaagaga ttgtccgtgc ggaagcttta ggtgctactc
    40441 aaatcaccct tcacccaggc gctcatgtag gagcaggggc agaagctggg attgctagaa
    40501 ttattgaggg gttgaatgaa gttttgacaa aagaacagac ggcacagatc gctttggaga
    40561 cgatggcagg gaaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg
    40621 atggagtaaa attgaatgaa aaattatccg ttacttttga tacatgtcat acgaatgatg
    40681 ccggatataa tataaaagaa gatttttcag gtgtaatgga agagtttgat aaggtcatcg
    40741 gattagatcg gcttaaagta ctgcatatca atgattctaa aaatcctcaa ggttctcata
    40801 aagatcggca tgccaatatc ggttttggaa caattggatt tgacacattg aacaaaatcg
    40861 tccatgatcc aatgtttgac aacatttcga aaatattaga aaccccatat gtcggtaaag
    40921 ataaaaagaa agcttacgca ccatatggaa aagaaatttc catgttcaaa gcgcaagcat
    40981 tcgatcccga agtgttccct gagttattag cagaagaagt cacactttaa gttttctacc
    41041 tgacagcaac aaaaaaggat tgggcagtct gccttgtcct tttttgttta agaaacaaag
    41101 gagtgctttt tgcattcgtt ttcgccatgc tccttttgcc ccctcttttt aaattcagac
    41161 aaacagggta tactgataga aaagtaaaaa acgaaaagtt tcacaaacaa gtagcaaaac
    41221 gtgattgaaa ggacgaaaag atgctgaaca ttttaaaccc cttgatcgac caactaaaaa
    41281 agaaacatac atactttatt cttggcatag ccgtattatt tacaattatt cggttgtatt
    41341 tatatagtaa agcaatctat ggaacagata tgtcggcgac ctatgatgat cagctgctga
    41401 ttcactatgc tcaaaatatt ttggatggta aatggctagg ggagtacacg actacgacac
    41461 tttcaaaagg gatttcctac agcttgtttt tagtacttgc acataaactg catctttctt
    41521 atgcagtttt attcggcatg ttcagtattc ttgccagtat attggttgca ctagcgttta
    41581 aaccaatcgt gaagaacaag tatcttcttt taagtatcta tttttatttt ttgttttctc
    41641 cgattagttt tacacatgaa tattccactc gaacatatcg taatgctctc gtgatcccga
    41701 ctgttgagat cattatcgct tgtttactag ctatttatta ccgcaaatat aattccagca
    41761 aaaaattgct gccgtggttt ttagggctag ctgtttcttt tcctttcttt tggttcattc
    41821 gcgaagattc catctggctt cttcctttta ctgtagtcgc gttaggcatc agtttcgttc
    41881 agatcttgct aggacaagac ttttctttca aagaagcaaa gaagttaaaa gaacgaccat
    41941 ttctcctttc gaaacaagaa tggctgaaac tttcattgtt tttactccct ctagtcggtt
    42001 tctccatcgt ctatcagtgg atcgaatata aaaacgagac aacatatggt attgcagcca
    42061 tcaatgaccg ttcgagtggc gaatttggac gtttgatgaa acagctgatc cgaatagatg
    42121 acgaaacaaa cttaaataaa gaaaacagtg agatatgggt ctcgcatgct gcattaaata
    42181 aagctttaga cgcatctcct acatttcata ctgtttctac tcgtattgac gaattgtata
    42241 cgactcatgc gtggacccaa ggcggaaaag taaaagaatt accaggagat atcatttttt
    42301 gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca aaagaaacca
    42361 atgatttttg gaaaaaagtc aatcaagaat tagaagaagc ctatcaaaag ggaactatca
    42421 aaaagaaaaa agaattctat ctgatgggat caggggatgg aaagacgatg aatgatctgc
    42481 caatcgtatt tgattttctg aaatcaggat atgcttatgc atggcgatac aaagatttcc
    42541 atcaaggggg gaattatagt tttgggacgc aagaacaagt cgaacaggca caagaattat
    42601 tgaagcttcc actaatggat aattggatcg atagcagtga tccgagaaca cctgatttcc
    42661 agttgacaaa aacagcaaaa gtcgcgaatg tcatgataaa aatttaccaa gttctggcca
    42721 ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga accgtatttg
    42781 caaaagctga tcgtgcctat tatagttcct ctttgattgt agtcatcgga ttaatactga
    42841 cacaattggt ttttcttttc ggtgtctcgt ggttttgttc ttatgcgcca gaaaaaaagg
    42901 attatttttt aagtgtctac actggcgcag gcgtgccatt aacgcaattc atcgcagtaa
    42961 caagcatgct agtttttagt aaattgaatc tgaaaaaagc tttatcttcc aaataaaaag
    43021 ggaactgtga caaaagtctt gtcgcagttc ctttttccga ataaacggtg
//
LOCUS       DAZKCN010000011        41435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000011 DAZKCN010000000
VERSION     DAZKCN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.011"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACG8K3_000572"
     CDS             214..498
                     /locus_tag="ACG8K3_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP9144476.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACG8K3_000573"
     CDS             complement(779..1198)
                     /locus_tag="ACG8K3_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP9144477.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ACG8K3_000574"
     CDS             complement(1200..1784)
                     /locus_tag="ACG8K3_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP9144478.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACG8K3_000575"
     CDS             complement(1831..3306)
                     /locus_tag="ACG8K3_000575"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP9144479.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACG8K3_000576"
     CDS             3487..3798
                     /locus_tag="ACG8K3_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144480.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACG8K3_000577"
     CDS             complement(3913..6249)
                     /locus_tag="ACG8K3_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9144481.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACG8K3_000578"
     CDS             6391..7059
                     /locus_tag="ACG8K3_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9144482.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACG8K3_000579"
     CDS             7062..8105
                     /locus_tag="ACG8K3_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9144483.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACG8K3_000580"
     CDS             complement(8342..9223)
                     /locus_tag="ACG8K3_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9144484.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACG8K3_000581"
     CDS             9382..10110
                     /locus_tag="ACG8K3_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9144485.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACG8K3_000582"
     CDS             10266..10583
                     /locus_tag="ACG8K3_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9144486.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACG8K3_000583"
     CDS             10576..11184
                     /locus_tag="ACG8K3_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP9144487.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACG8K3_000584"
     CDS             11239..12030
                     /locus_tag="ACG8K3_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP9144488.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACG8K3_000585"
     CDS             12168..12656
                     /locus_tag="ACG8K3_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP9144489.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACG8K3_000586"
     CDS             12707..13579
                     /locus_tag="ACG8K3_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9144490.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACG8K3_000587"
     CDS             13755..14117
                     /locus_tag="ACG8K3_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9144491.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACG8K3_000588"
     CDS             14126..14878
                     /locus_tag="ACG8K3_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144492.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACG8K3_000589"
     CDS             14892..16694
                     /locus_tag="ACG8K3_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144493.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACG8K3_000590"
     CDS             16971..18011
                     /locus_tag="ACG8K3_000590"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9144494.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACG8K3_000591"
     CDS             complement(18063..20207)
                     /locus_tag="ACG8K3_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9144495.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACG8K3_000592"
     CDS             complement(20564..21937)
                     /locus_tag="ACG8K3_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9144496.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG8K3_000593"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG8K3_000593"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9144497.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG8K3_000594"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG8K3_000594"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9144498.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACG8K3_000595"
     CDS             complement(23385..24398)
                     /locus_tag="ACG8K3_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9144499.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACG8K3_000596"
     CDS             complement(24479..25078)
                     /locus_tag="ACG8K3_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9144500.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="ACG8K3_000597"
     CDS             25264..25965
                     /locus_tag="ACG8K3_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144501.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="ACG8K3_000598"
     CDS             25975..28686
                     /locus_tag="ACG8K3_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP9144502.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="ACG8K3_000599"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="ACG8K3_000599"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9144503.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="ACG8K3_000600"
     CDS             complement(30825..32006)
                     /locus_tag="ACG8K3_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9144504.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="ACG8K3_000601"
     CDS             32258..33781
                     /locus_tag="ACG8K3_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP9144505.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="ACG8K3_000602"
     CDS             complement(33850..34578)
                     /locus_tag="ACG8K3_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9144506.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="ACG8K3_000603"
     CDS             complement(34591..35871)
                     /locus_tag="ACG8K3_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9144507.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="ACG8K3_000604"
     CDS             complement(36012..36614)
                     /locus_tag="ACG8K3_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9144508.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="ACG8K3_000605"
     CDS             36859..37065
                     /locus_tag="ACG8K3_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144509.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="ACG8K3_000606"
     CDS             37377..38096
                     /locus_tag="ACG8K3_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9144510.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="ACG8K3_000607"
     CDS             complement(38177..38860)
                     /locus_tag="ACG8K3_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP9144511.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="ACG8K3_000608"
     CDS             39078..41264
                     /locus_tag="ACG8K3_000608"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9144512.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13444 a         7345 c         8312 g        12334 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccatat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagttg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taaaggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatgc tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaagatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggaa cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatatc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgtga caagaatttt gtcacacctc cctta
//
LOCUS       DAZKCN010000012        38021 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000012 DAZKCN010000000
VERSION     DAZKCN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.012"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..278)
                     /locus_tag="ACG8K3_000609"
     CDS             complement(<1..278)
                     /locus_tag="ACG8K3_000609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144513.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVT"
     gene            complement(415..1068)
                     /locus_tag="ACG8K3_000610"
     CDS             complement(415..1068)
                     /locus_tag="ACG8K3_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144514.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1118..1546)
                     /locus_tag="ACG8K3_000611"
     CDS             complement(1118..1546)
                     /locus_tag="ACG8K3_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9144515.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(1551..1925)
                     /locus_tag="ACG8K3_000612"
     CDS             complement(1551..1925)
                     /locus_tag="ACG8K3_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9144516.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2228..2413
                     /locus_tag="ACG8K3_000613"
     CDS             2228..2413
                     /locus_tag="ACG8K3_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144517.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            2426..2764
                     /locus_tag="ACG8K3_000614"
     CDS             2426..2764
                     /locus_tag="ACG8K3_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP9144518.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2751..2930
                     /locus_tag="ACG8K3_000615"
     CDS             2751..2930
                     /locus_tag="ACG8K3_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144519.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2973..3443)
                     /locus_tag="ACG8K3_000616"
     CDS             complement(2973..3443)
                     /locus_tag="ACG8K3_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144520.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3530..4228
                     /locus_tag="ACG8K3_000617"
     CDS             3530..4228
                     /locus_tag="ACG8K3_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP9144521.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4406..4747
                     /locus_tag="ACG8K3_000618"
                     /pseudo
     CDS             4406..4747
                     /locus_tag="ACG8K3_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4740..5411
                     /locus_tag="ACG8K3_000619"
     CDS             4740..5411
                     /locus_tag="ACG8K3_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP9144522.1"
                     /translation="MSENKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5417..6103
                     /locus_tag="ACG8K3_000620"
     CDS             5417..6103
                     /locus_tag="ACG8K3_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP9144523.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            6110..6961
                     /locus_tag="ACG8K3_000621"
     CDS             6110..6961
                     /locus_tag="ACG8K3_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFP9144524.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            6973..7242
                     /locus_tag="ACG8K3_000622"
     CDS             6973..7242
                     /locus_tag="ACG8K3_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144525.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            7247..7411
                     /locus_tag="ACG8K3_000623"
     CDS             7247..7411
                     /locus_tag="ACG8K3_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144526.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            7404..7706
                     /locus_tag="ACG8K3_000624"
     CDS             7404..7706
                     /locus_tag="ACG8K3_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144527.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            7703..8014
                     /locus_tag="ACG8K3_000625"
     CDS             7703..8014
                     /locus_tag="ACG8K3_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP9144528.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            8011..8229
                     /locus_tag="ACG8K3_000626"
     CDS             8011..8229
                     /locus_tag="ACG8K3_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFP9144529.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            8240..9055
                     /locus_tag="ACG8K3_000627"
     CDS             8240..9055
                     /locus_tag="ACG8K3_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFP9144530.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            9057..9182
                     /locus_tag="ACG8K3_000628"
     CDS             9057..9182
                     /locus_tag="ACG8K3_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144531.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            9194..9550
                     /locus_tag="ACG8K3_000629"
     CDS             9194..9550
                     /locus_tag="ACG8K3_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP9144532.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            9510..9755
                     /locus_tag="ACG8K3_000630"
     CDS             9510..9755
                     /locus_tag="ACG8K3_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144533.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            9752..10150
                     /locus_tag="ACG8K3_000631"
     CDS             9752..10150
                     /locus_tag="ACG8K3_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP9144534.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            10147..10485
                     /locus_tag="ACG8K3_000632"
     CDS             10147..10485
                     /locus_tag="ACG8K3_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144535.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            10482..10712
                     /locus_tag="ACG8K3_000633"
     CDS             10482..10712
                     /locus_tag="ACG8K3_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144536.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            10719..10922
                     /locus_tag="ACG8K3_000634"
     CDS             10719..10922
                     /locus_tag="ACG8K3_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP9144537.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            10919..11224
                     /locus_tag="ACG8K3_000635"
     CDS             10919..11224
                     /locus_tag="ACG8K3_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144538.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            11208..11501
                     /locus_tag="ACG8K3_000636"
     CDS             11208..11501
                     /locus_tag="ACG8K3_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144539.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            11761..12177
                     /locus_tag="ACG8K3_000637"
     CDS             11761..12177
                     /locus_tag="ACG8K3_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP9144540.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            12439..12699
                     /locus_tag="ACG8K3_000638"
     CDS             12439..12699
                     /locus_tag="ACG8K3_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144541.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIAIVNKAE
                     SENSSFFVKANWNIDLPEGDINNPVAEPIKYNPSKAKLDKVH"
     gene            complement(12653..12901)
                     /locus_tag="ACG8K3_000639"
     CDS             complement(12653..12901)
                     /locus_tag="ACG8K3_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144542.1"
                     /translation="MKITAAITKEQGVTFTVVVVKNGVISSSHREQVRSSIPRNFPRP
                     IILAEQSHGKMKYHGRTDIVKFLSNVPYQALPWKDYTL"
     gene            13260..13472
                     /locus_tag="ACG8K3_000640"
     CDS             13260..13472
                     /locus_tag="ACG8K3_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144543.1"
                     /translation="MNEKQLSELFKLNESNQTAEATFYEAQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            13479..13706
                     /locus_tag="ACG8K3_000641"
     CDS             13479..13706
                     /locus_tag="ACG8K3_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144544.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            13703..13987
                     /locus_tag="ACG8K3_000642"
     CDS             13703..13987
                     /locus_tag="ACG8K3_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144545.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            14028..14600
                     /locus_tag="ACG8K3_000643"
     CDS             14028..14600
                     /locus_tag="ACG8K3_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144546.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            14601..14912
                     /locus_tag="ACG8K3_000644"
     CDS             14601..14912
                     /locus_tag="ACG8K3_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9144547.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            14903..15163
                     /locus_tag="ACG8K3_000645"
     CDS             14903..15163
                     /locus_tag="ACG8K3_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144548.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            15367..15885
                     /locus_tag="ACG8K3_000646"
     CDS             15367..15885
                     /locus_tag="ACG8K3_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144549.1"
                     /translation="MKQKRIEKGLTQADVATKSGISSRYYGSIENGKNSPSIETLNSI
                     SIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVEGLIIQAARLRVL
                     LDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAIVKQLTDLLPQGT
                     RADKKSKLLGRK"
     gene            15888..17579
                     /locus_tag="ACG8K3_000647"
     CDS             15888..17579
                     /locus_tag="ACG8K3_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9144550.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            17611..18558
                     /locus_tag="ACG8K3_000648"
     CDS             17611..18558
                     /locus_tag="ACG8K3_000648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFP9144551.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLFKSIQDKMNS
                     ELFSTIEKRIKVPINNINTNLDVRENKETTKEVQEDLKEYSASLNKVLSESVKKINEI
                     FEDNYKDIGSYLKVFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILIN
                     SLFTLRGTGMGIELIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKT
                     RKKNRKLFENRKINDNLLSLLRIRYTLQDGNKIIISNGV"
     gene            18607..19779
                     /locus_tag="ACG8K3_000649"
     CDS             18607..19779
                     /locus_tag="ACG8K3_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9144552.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            19766..20470
                     /locus_tag="ACG8K3_000650"
     CDS             19766..20470
                     /locus_tag="ACG8K3_000650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP9144553.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            20475..21635
                     /locus_tag="ACG8K3_000651"
     CDS             20475..21635
                     /locus_tag="ACG8K3_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9144554.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITSSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            21711..21989
                     /locus_tag="ACG8K3_000652"
     CDS             21711..21989
                     /locus_tag="ACG8K3_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFP9144555.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            21970..22341
                     /locus_tag="ACG8K3_000653"
     CDS             21970..22341
                     /locus_tag="ACG8K3_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFP9144556.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            22357..22761
                     /locus_tag="ACG8K3_000654"
     CDS             22357..22761
                     /locus_tag="ACG8K3_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144557.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            22758..23168
                     /locus_tag="ACG8K3_000655"
     CDS             22758..23168
                     /locus_tag="ACG8K3_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144558.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            23229..23816
                     /locus_tag="ACG8K3_000656"
     CDS             23229..23816
                     /locus_tag="ACG8K3_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP9144559.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            23889..24356
                     /locus_tag="ACG8K3_000657"
     CDS             23889..24356
                     /locus_tag="ACG8K3_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFP9144560.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            24402..24710
                     /locus_tag="ACG8K3_000658"
     CDS             24402..24710
                     /locus_tag="ACG8K3_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144561.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            24905..31669
                     /locus_tag="ACG8K3_000659"
     CDS             24905..31669
                     /locus_tag="ACG8K3_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9144562.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            31673..32494
                     /locus_tag="ACG8K3_000660"
     CDS             31673..32494
                     /locus_tag="ACG8K3_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFP9144563.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            32503..33546
                     /locus_tag="ACG8K3_000661"
     CDS             32503..33546
                     /locus_tag="ACG8K3_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9144564.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            33546..34598
                     /locus_tag="ACG8K3_000662"
     CDS             33546..34598
                     /locus_tag="ACG8K3_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144565.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSTNSRI
                     DNLVLHSGGDSPNEVVDARVNHKGETFATLQGRLTDTEKKVSESVSTLEKNQADIKAQ
                     VAQLNNSVETIIGGSADWINLYVSADLGSDQIGDGSEEKPFATIQMAVNQIPLVSIPG
                     IRIWVDDGVYLEDVFIRNVSATTIHIGPKNDTSVIDPSKSDMPVKLRSLAFYQCKGFF
                     KVTGLQFVDTINAPKNSGLIYSLMLLQGGYLSVDKCKFAEDNRNLTSAAIYTEGLSAS
                     NVYNTCYFYRQNIVVYANLMSQVLISQQTSGKENTTGARSKDAIIRGKFSVGFADKNE
                     DVKDLGLIITKGTVLS"
     gene            34598..36781
                     /locus_tag="ACG8K3_000663"
     CDS             34598..36781
                     /locus_tag="ACG8K3_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP9144566.1"
                     /translation="MAFKINESIIVIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMFRSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDAKLKNTNDKITKANQDVTTLNTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLLSKLSYDLIENQNTSAGTLSKGENSFKYNKISAE
                     VEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKI
                     VLDLPINSNSITQEWKEFSITGTTPTFSPQAYHPWIQFRAQDGILGEIEMSYDIKIEE
                     GSTATPFQPNLLAEPYNMCREYPNENIADHTVKFPIESGDHQIYQGYTEEELMIGQTY
                     TITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQY
                     PRSTVGACQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYAEKGLN
                     NTVSLTEPETVLGLKNFKDGVQIDGVDVATVKNLGSVLQSGSGLTTIQSDKQSAFDEF
                     SYEFERIGDRVFFHARVKTNSTTAVASHVNLLEIPLGFQFPIGWTIVGVPLSVVQWTT
                     PQGEISALVITDSTGKQVVKFATNRVGNHYISGEWRTADSYPETK"
     gene            36793..37239
                     /locus_tag="ACG8K3_000664"
     CDS             36793..37239
                     /locus_tag="ACG8K3_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144567.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            37241..37378
                     /locus_tag="ACG8K3_000665"
     CDS             37241..37378
                     /locus_tag="ACG8K3_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP9144568.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            37413..37655
                     /locus_tag="ACG8K3_000666"
     CDS             37413..37655
                     /locus_tag="ACG8K3_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFP9144569.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            37671..37868
                     /locus_tag="ACG8K3_000667"
     CDS             37671..37868
                     /locus_tag="ACG8K3_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9144570.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            37879..>38021
                     /locus_tag="ACG8K3_000668"
     CDS             37879..>38021
                     /locus_tag="ACG8K3_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9144571.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQ"
BASE COUNT        13528 a         5856 c         8053 g        10584 t
ORIGIN      
        1 tttgttacct gttcagtttt tatagaagaa attgtatatt ctaggatacc aacaactggc
       61 aaggtttttt ccttagaagc attgtcgata gattcgctat tttcttcagt gtcatttgac
      121 tgcgatgcta tttgtttttt aagctcttca ttttcttttt ttaaactatt gattgttttt
      181 tgagtttcac tattagttgc attatttgaa ggcgaactac atgcacccaa agttaaaata
      241 ctagtacaaa taaatcctaa tattaagttt tttttcattt ttaattcctc acttctatga
      301 tattattttt atgtagaatc ttagaaatga gattttagtc cgtgttgcag cacgggcttt
      361 tttctttata actttttaga ggttataggc aagatagtgg gacacaaaaa tatattattg
      421 tattccgtat ttagaaaatc ctaattgaac ttcaccggaa gttttttgct gtgttgtacg
      481 caatgcttct tcttcagaca ttccattctg tactttccat gcaacaggcg acatcccgta
      541 tttgttaaca aaatcagtaa gtgataaagt gtcagcgtct tgttgagcgc ttgtttgttg
      601 ggcttctgta ttttgttgag atgcagcttg ttgttctttc tgatcttgac tgataatatt
      661 gccagcatca tctgtagtca atccattttc ataaagggcc acgccgaaag cttcccactc
      721 tttgttggac caatttgcac gatcagctgg agttgactgt aaagtgcgtt gtttcatctg
      781 ttcatatgtt tcttcttgag gtgcggtttg gattgtatcc tgactggagc ttataactgt
      841 tgggctaggt ttcgccgtag cttggttgga gcttgtaact gttgaactag aatctgtttt
      901 agatgtagat ttgctagtag atgaactgat ttcagttgtt tcttttgttt tacttatatt
      961 tgtttcttgg ttagaagtgg catctgttga ttcagctttt ttattatttg aacaagctga
     1021 aagtagcaga gcagtactta acaacaacat aacgctaact tttttcattt tataattcct
     1081 ctttctcgtt gtaatatgtg tgctaacacg ggctttttta tataagaaaa cgataagcgc
     1141 tttctggaag accgtaaaga ttctttaatt cctcgatttt tttaggatat tgatcattat
     1201 cttctttata aagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt
     1261 tgcttctaga tgttcttgtt gatacataat agctggataa gccacgatga aaaatagcgt
     1321 gacctaattc gtgagcgcaa atgtagaatc tttcctcaga gtctcgcagt tcatcattta
     1381 aaaagattat cgcacgatct ctaatttctt gaaactgccc cttgggattc tcgataaaag
     1441 gaacgtattg aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt
     1501 atttccgctt caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt
     1561 ttgccttttt ctttgtcttc tttcacaata tcccagaaag tcgctatcag gatatctttt
     1621 acgcgctgta tttgttcggg tgtcaatgtt tccccaccat aagacatatt aacatttgag
     1681 tctagtagtt tatcaagttc aaccacttcc tcttttgtag cccatttggg aacattatta
     1741 tttcccaata aataatcagt tgtgacacca aaataatcag caactttctt taagttctca
     1801 gattttggcg aggctttatc ccatcttctt atttgtccat tagagatgcc cacctgtctt
     1861 tctacttctg ctatagtcac atgcttttcg tctgctaatt ctttaatctt agtaactaaa
     1921 ctcattatta tcaacctttc aaagctgaaa agaaaataaa tagcttaaaa gttatatttt
     1981 tggttgacaa ttagctttta agctagtata tttaattcgt aagctaaatt gttagctaaa
     2041 taagagcaac aaaaaactct actaatttaa aacattctct cggtcgccaa acttagaaat
     2101 gttattttag aggcttttta taagtcttat ttaactatgt attcataata gcttaaaagc
     2161 taataggtgt caacgattta gctaattttt tagcttacaa attatttttt agaaaggagc
     2221 tatttttatg tctgagaatt tagacttaaa aattcgagca gagatgagaa aaagaagaat
     2281 gactttcaaa gaactagctg cgcttgttgg tatttcagga gcttatttat cagatatcct
     2341 aaacggcaat cgtgatggta aaaaagctca agaacacata gaaactgtaa aaaaaatatt
     2401 aggtattcaa taggaggaac agctaatgca atatctagaa gcgaaaatcc cagttccaga
     2461 aggctatgta attatctctc aagtggatta cgaggagtta aaaaaagctg atgatactgg
     2521 tagatggatg acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac
     2581 ttctagagtt ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa
     2641 tggatttgtc tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac
     2701 acttgaattt ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat
     2761 ttaatagagc gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa
     2821 gcaggcaagc gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg
     2881 aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa aaatgaatag
     2941 aaaagagaaa ctagaatgta tattactatt actcagttta attctttcac taatttctct
     3001 attgggaagt ttgtattttt gatatcaaca attttgtgga tttgggttct atttagagta
     3061 aatatcattt ttgtttggtg attaaggagt ttattaaaat taattttccc tacttcaaat
     3121 gcaaggtaac aagatatagc acttttagga ggaatattta gcggtacagc ttcagagtat
     3181 attctattat catcgactga tcctccttta gcaattaatt tcttaaatgg agtagctgac
     3241 catctgcggt ttaagttagc ggatgaaagt tctaaattaa ctaacatagc tggttctgtt
     3301 gaatagttag aaatgattac ttttgtataa atcatatctg cagcgaagta agaagcatta
     3361 agttcaactt ctagttgagg gcgcattcct tttattttta tccctgttaa aaacgtactc
     3421 aatataaaac ttattattga catccattga aaaattgtta agtgaaaaaa attcaaaata
     3481 aacaccacca gtttttaact aaattatacc aaaaaggaga gaagaaataa tgcaagaatt
     3541 agtaattttg aaaaataaag aagctgtgac tacgagctta caagtggcag aaagcttcga
     3601 gaaaaaacat caacatgttt taagagatat tgatgcacta aaaaaagatg tgtccaattt
     3661 tggacagatg tttgtagaag gtaatgaacc agattcatat ggcagaaatc gacgagtttt
     3721 cttcattagt agagatggat ttttcttgct ggctatgggg tttacaggaa agaaagctat
     3781 ctacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta ttcgcaagaa
     3841 tactgttcct caaacaattg aagatatgat gatctatcaa ttagaagaaa tgaaagatgt
     3901 taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac aagagtttga
     3961 aattaagcaa aaaggaaata tgaaagttat gggagttcta gggggaaaag aaagccgagc
     4021 ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
     4081 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
     4141 tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt gatcaactga acagacaaca
     4201 gagacttttt ggtgatgaca atgaatagag ctgaagcgct aagaataggg acggcaattg
     4261 ctaattgctg gtggaaatac tataaaccaa tcatcctaag ccaacaacat attgacaagc
     4321 aaaaagcatg gcaacaaata aaaaagtgac tccgccggca agcaaagagt cacaaagaaa
     4381 acacatcata aggagatttt agcatatgga aaaagaactt tccactctag atcaatattt
     4441 gattgatcct gattggggca agccgaaaat tgaggaaaca agtggtcgaa aaatcaggcg
     4501 aaatcttttg acgaatgaag aactagcttg tgatcaagat gatttaggta accttgtaac
     4561 tatttgagat catgtttatc ttatccattt atcgaagcat tcgaataaac ctgaatatat
     4621 ttacgtcatc gaagatggct tgattgatgc attagaagag tacgaaaggg ataacttgat
     4681 cgatatctct tattacggac caggtaagaa atacattgct gaattggagg cagaatttga
     4741 tgagtgaaaa caaagggaca acgaattttg aaaaactttt tagtcgcaag ttaaataaaa
     4801 ttctcaagaa aaaaggaaat tttgattatt tatcttgggc tcacgcatgg gagattatga
     4861 aaaagaatga tccacaggca acagtaacta ttaatgagta caaacactac agagttgttt
     4921 ctggaactca tcaagacttt cttgttgagg aatacaaacc ttttcttatg gatgaaacag
     4981 ggacttatgt atctgtctca gtaacggtta aaggacacac ggaaaccgag ttatttcctg
     5041 ttttagatta tcgaaaccaa ccagttgtta aaccaaatgc aatgcaaatc aataactcat
     5101 tgaagcgatg ctttgtgaaa gcattggctc tacacggact gggattatat gtatttcaag
     5161 gggaagatat tccaacacca cctagaatcg atacgaagaa attaaacatg ctagagacga
     5221 ttctagaagc tttcaatgag cagatgggta aagatatgac caaaacctta atcgaatatg
     5281 ttaatgagca gacagataaa ttagggctct tagctgataa cgttgaaact attgaacagt
     5341 taagctatga gcaatgtgcc ttgatggagc gagcaatagc agctaagaga aaagaattag
     5401 ataagaagtg atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa
     5461 gttcaaagga aaagacatag gtgcaaccat caatgaggaa gtgaacattg atcgactaaa
     5521 gacgatgtat gacggctacg atggtgatcg agttattgaa attcgtttta ttgatcctag
     5581 acgtttcaca gtacagcaac gaaacttcat ctatgcactc ataggcgata ttttcatcga
     5641 tacaggcatg ccaacggact tctggaagga attcttttac ttccgctttg aaggtgtcac
     5701 agggcgcaaa ataagcctca aagatgaatc gaatacgact gtgagtgatg ctaacgtctt
     5761 agcaaatatc atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga
     5821 gattttacca gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgttg
     5881 catctgtggc aaaacaggag ctgatatcga tcactttgac aaagcgctag gaagacgaaa
     5941 gcgcaaagaa gttgatcatt cagagtacac atttgcagca ctctgcagaa tccatcacac
     6001 ggagaaacac aagataggtg tgatcaattt caaaaataaa tatcaaatca aagggatcaa
     6061 gttaaaccag gaaacaatta agaaacttag aataggagga taaaaaatat tggctgacaa
     6121 caaacgatac tactatttaa aactaaaaga gaactttttc gatagcgacg agatggttct
     6181 tttagaaagt atgccagatg gatacattta ttctaatatt cttctcaaac tttatttaag
     6241 aagtctgaaa catgaaggca aactaatgtt taatgacagg attccattca attctacaat
     6301 gcttgcgact attacaagac actctgtggg agttgtagaa aaagcagtac aaatatttcg
     6361 tgatttacaa cttattgagg tattagataa cggagcaatt tatatgtctg atatacaaag
     6421 ttttattggg aaatcttcaa ctgaagctga taggaagaga aaatacagaa aagaaattga
     6481 ggaagcaaaa cagaatttaa taacaagtgg acaaatgtcg gacaaatgtc cggacaaaac
     6541 tacaccagag ttagagatag agttagagaa agatatagag ttagagaaag atatagagaa
     6601 tgtaacgcct tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg gagagtataa
     6661 aaatgttctt ttgtcagatg agcagatgga gaaactcaaa atagaattcc ctaatgacta
     6721 tcaagaacga atagaacggc tatctgagta ttgtgaatca tctggtaaga cttataaaaa
     6781 ctatttggca actattcgaa gttgggcaag gaaagaaaaa agtgagccta agaatgcaag
     6841 tggtgcatac aagcgcacag gaagacgaga gaagcttcct gaatgggcaa tcgaccaaga
     6901 agcctatctc aagaaaaaag cgctagaacg agctaataga caatcaaaag caccatttta
     6961 agaggtggaa aattgaaaat cgattatcta gaactaatta atgaaatagc aaagtataaa
     7021 actggtgagg aaatagaaat cctgagagac gtatatgatc aactcgaaga agctggaatc
     7081 gaaggaatta ataatgatcg ttcgagttgg agtaaactca gatactattt cgcactctat
     7141 atcgatacaa cacaattaag aaatttagct tatacaaaat tactatttgt tgattgcgtt
     7201 aaaggattgc aaaaacatct tagtgaactt gagcaggtgt aatcagatgg atctaaaaac
     7261 atttacagca cagatcgaac taatgcatca agaagcttta agacaaagcg cctcgtacga
     7321 agacaagtgg ctcaacacgt tccatggtgg acgtgagagc gcacttgatc aagtactcaa
     7381 attattgaaa ggggaacgtc gggatggata agaaagcggc aatgaaacga atcatcgaac
     7441 tgacacattc tgagaattgg caagaagaca aagaaatagt tacagaagtc caaaagctcg
     7501 gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc gagaaagatt gcaatttggc
     7561 atggtgatcg aattctagta acaggtactg ctgaacagtt atctgaaatt actggtctga
     7621 gcaaaaacat catctgggat agagcaagga gcttatggat tgattcaaaa ggacgacaat
     7681 ttaggtatgt ggaggagaaa aaatgaacga actaatcaca aaagtagagc agtgggctaa
     7741 agacaaggga ctggatcaag cagatccaaa agcacagttt ttgaaagtag ctgaggaatt
     7801 cggggaaatc gcttcagcaa tggcaagaag taatgatgag ctatttaaag atagcgtagg
     7861 agacgtaatc gtcacgttga ttatcctttc catgcaaaaa gggacaaacg tacaagagtg
     7921 tttagaaatg gcatacaacg aaatcaaagg gcgcacagga aaaatggtag atggtgtatt
     7981 cgtgaagtcg agtgatttgg aggacagcaa atgataggtt taacaatatt ttggtgtgcg
     8041 attggtggtt tactagcatt tctaggatac tacctcatct gtgaaagtag aaaaggaaga
     8101 aaatatacag tgacaggtat tgtcctgtta attatcggaa ttctagttgc agttggagca
     8161 gaactgaatc tttatgtaaa tggtacacaa gaagatttgg tgcgatttat gttttggatg
     8221 aagaattagg aggaaaaaaa tgaacgaaaa caaattaatc aaattaggtg tagcaggagc
     8281 agtaatcgta ggtattggag ttatcggagg atttaagttc ttcgaaaaaa tcgataatgg
     8341 atatgtgggt gtgcgctatt caatgaacgg cggtatcaaa gatgaagcac tgacgcaagg
     8401 tgtgaaattt gtagggattg acaaagtgat ccaatatcca attcgcttgc aaactatcca
     8461 atcaaaaaat atttcagtat ctacaagcga cggcaaaaag acaacgattg atatcaaata
     8521 tgactacaaa gttgattcaa ctaaagcagc aaaaatgtac aaggaatttg ggaatatcac
     8581 ttcggaagat atcgaaagtg gatggttaaa atctaagctt caaaaggtcg ctcgtgaagt
     8641 ttatgcgaaa tatagtctgc ttgatgtcct ttcaggagat tcctctaaag ttgaagctga
     8701 ggtattaacg aactttgcta aatcagttga atctaaaggg tttgaagtcg aagacgtaac
     8761 acttggtgtt ccagatgtcg ataaagaaac acaaaaatca atcgatgcga tcattcgagc
     8821 tggtcaagaa aatgaaaaag cgaagctaga tgcagaaact gcaaaaactc aagctgatag
     8881 tgaagcttac aagaaaacaa aagctgcaga agcagaggca gaatctaatc gcaaagtcgc
     8941 cgaatcagta acagacaatt tgattcgtta tgaagaagct caagctcgca aaaagcatgg
     9001 atgggtaaca gtaaatggag cagatactgt agttacggat gaagcaggca aataatatgg
     9061 gattctttat ggctaaaatt ctcttgttct taggtttagt aggagcagca tatctcgtgt
     9121 atgcgctcct ttcccaaact gatgacaaag aagatgacaa caacgatgaa atgaaatttt
     9181 aggaggagaa ataatggatc tcattacaca atacagtgat atcatcctca agaaaatcat
     9241 ggcaaagatt cagaaagata aaaaagcaaa agaacgagcg gaattagtta agttggaaat
     9301 ggctgaaaca ggagtaggag tgcgaagtag caggcattgg aaagcagcag caaacattga
     9361 attttattac aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg atcggcaaac
     9421 aaattggagc aagaaacttc atcaagatcg tttcaaattt gtagagaagt ataaagaaat
     9481 attagaagag tatttgagga ggacagcgaa tgataaaaaa gctcattcaa ttcagcatgg
     9541 atttatatga tatcgaatca ggagcaacag tatctgtgga gtctgatcat ttaatcataa
     9601 gttttgctga cgaacgtcaa attatcatat gggtagttga cgatatgcta tatccagaaa
     9661 ttgtacatga ttttgaagaa tcaaaagcgg ttgagtttga aatagtgaaa aaagtaatgg
     9721 aattgattga aaaatacgag gaggacagca aatgataccg aagtttagag cgtgggataa
     9781 gcgagaaaac acaatgagag atgtagctgt cttacatttt actaaaaacg gcaaaacaaa
     9841 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
     9901 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gagatatttg aaggggatgt
     9961 agtaattaca tcaaaaggag cgattggata tgttgcgtat cttcaacaag aagccggctt
    10021 tgtagttgta ctaaagaaaa gtgattatag attaggccat aggaatactg gcgaaagtta
    10081 tgatgtagca actgctcatg aagtcatcgg aaatatatac gagaatagcg agttattgga
    10141 ggtagagtga ttatcttagc tactgactac atcgattctt taaaagatga aaatgggaat
    10201 gtcccagcat tgctaaattg tggaattaaa agcaagaata gaatcatttt gttacggata
    10261 tcggctgatt tagcaaaata tctttatctg agcaagacag cagtatatta cactgcggct
    10321 acacgttatc agtacaaggg aaaaacaatc agtcaggatt attatgatcg tttaatcagt
    10381 cctgacatgc atggaaaatc aaaatcagcg atcaagttat ttggcgcaat agagatatat
    10441 gccgatgatt ttccaaattt atggttgaag gaggaagcgg aatgaaatac gaaataccac
    10501 taagtgaagc gggcattcaa gcaattatca atggtcggga ggttaataaa aaacttcctg
    10561 atggtactga attagtcatc agacaaagtt atttgaaaga tatggcagct ccagtattaa
    10621 ttgatcgttt taacgtgact gattctgtgg tagagaacca cttgaaagaa tttcgatcaa
    10681 gtataaacga cactttcaga ttagggagtt gattgacaat gaacaccaga caccgcagag
    10741 tagcaaaact aagaaaacag gaactgaatg tactaaagac aaagtttgaa aaagaatatg
    10801 gaatttcagc agaagaaaca tataaagtgg caagtcagtt tgttgctgat gcgagtgaga
    10861 ctattcgtaa gtttgggatt tcgatattaa atgatgatcg taaatgggag gcagaaagat
    10921 gaaactaaaa gacggatttt acactgatgc ttatggaatt ggtggtttga tgatggatct
    10981 accaactaag aatcctataa agcaagagga atcagaaatt aaagtcggtg acatggttcg
    11041 ctgcgaagca gaagggttca tctatccatt tcgtggatat gtagagcatc tctataatca
    11101 ctcagcgatc attcgtattg aaaacacaat ggaatgcgat aagtggttag cgaaaagcaa
    11161 agagaattta gcagtggtga gattggtgga tatggaggtt ataaacaatg gaattaaaaa
    11221 ttgacaaggt aacgtttgaa gaaccagcat ttcaagaaat ggatgaacga attgcagaat
    11281 tggtcgagaa aaaacaagat gaattgattg ctttgctaga aaagaaatac caatttccga
    11341 aatatatgaa caaaatgcaa gcagcaaaat atcttggtgt aagttacaac acgatgatgg
    11401 ataagtacgt accagctggt ttgaaaatgg gtatcgttaa cggcgttatt cgtatcagca
    11461 aagaagaatg tgatcgtttt atgaaagaac atcaaaaata aaataaaaaa acgtttagcc
    11521 ccttagctaa acgcacaatt ataatttacc tgacagttaa ttataacaaa attgcggagg
    11581 ttgaggaatg gcggaatgaa aaagacaata aaaacagaag aagcatatat tgaagtattt
    11641 gacacaccag catgctaagt cgagaagtgt aactcggcaa ataaaaaaag ccggatcgct
    11701 ccgactgatg taataaaccc aacaagttta ttatatcaca taaaggagcg gtttgacttg
    11761 atgcaattgt tacgagaggt agatttcaaa cagacaagat gcaatgcgag agatgtgctg
    11821 aagaattttc gacgcttaga gcggatggca ggtcgctctt tgatagatat taagtcgccg
    11881 attattacgg atatgccgaa ggcaccgaag cacggcaata aggcagagga cgcgattatt
    11941 cagatgatgg atatagaagc agagagagat gcgattctag cggctttgat ggctcttagt
    12001 ctgattagcc gtcagatact ctattatagc ttttgtgacg taaacaagca ctctaattat
    12061 gaaatagggc aattgatacg aggatacgga gagaagaatg tagagaagct gaaatctatc
    12121 gcgctgatcg aatttgctga agcatacaaa aaaggcgtat tagttcagta tcgttgattt
    12181 tgtagggttt ttgtagggat agtgtagggt ttttgagtgg tttaacgtga tattatgata
    12241 gtgtcgaaag attagtgata ggtctaagac aaaataataa taaaaggaac atcgttttat
    12301 tattgtttca caattaagct tcgatagaca gcagcggaaa tattaagaat aaggatgtga
    12361 attttaactc cttctaaatt gttcttatta tctatcatcc gttgctgtct attaatttat
    12421 gtattggagg gaaaacgaat ggataaagaa atcaaagtaa ctgtcaaatt agatttgact
    12481 gaactaaaag aactgctcaa caaggctagt gaccaagtcg aacagttaca agaaacttta
    12541 gatgaaattg ctattgtgaa taaagctgaa agtgaaaatt ctagtttttt tgtaaaagca
    12601 aattggaaca ttgatttacc agaaggggat atcaacaatc cggtagcaga acctataaag
    12661 tataatcctt ccaaggcaaa gcttgataag gtacattaga aagaaatttt acgatatctg
    12721 ttctaccgtg atacttcatt ttcccatgcg actgttcagc taagataata ggtcttggga
    12781 aatttcttgg gatgctactt ctaacttgtt ctctatgaga agaagagatc acaccgtttt
    12841 tcacaacaac aactgtaaaa gttacgccct gttctttggt aatagcagca gttattttca
    12901 tatattcacc acctataatt tatttcagcg gaccactcgc tgataaataa aattatacgc
    12961 ttagtattta ttttcacaat attaatttgt cactgtggcg gaaagggtag acgctaagca
    13021 tgcgtgctag gtcaatgctt cggcaaccat gcaatgttcg attcattgcc agtgactttg
    13081 gcaaccgagg gttggaaatg ggcgctccaa gtacacgagc aaggcgaggt cgatagtaat
    13141 cgatggaatc ggtgtaggtt gcttgataga actataactg catctcgttt atgaggtgta
    13201 gtttttacat attagatcac tctttgagtg gtctttttat tttgaaagga gttttatcta
    13261 tgaatgaaaa acaattaagc gagttattta aattgaatga atcaaatcaa actgcagagg
    13321 caacattcta tgaagctcaa aaaagcttag cgttaatcgc aaaacaagct aagtatttct
    13381 atgaccagct tattctacaa gggtttaatg aaggacaggc tatggaattt atgatgcgaa
    13441 ccttttctgc cagtaaccaa cagaaagagt gatacataat gagaaactac tggtatatat
    13501 cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt gtgcgttctg
    13561 tacaaatcaa aaagaagtac tccatcattg aaatgaccag agaagctaca ccaaatgaga
    13621 tcgataagta caatcttcgt tactgtggcc atggatattg gaaagacgaa tatattcaac
    13681 aaaacattga gaggtacatc aaatgaatga taactatgat tacatcaagt tgattgaaaa
    13741 gataagagca gaaaaagata tggacgaact agctaactta ttcatgaaca tcattagttt
    13801 ggtggggtta aagatggatg aagtagcggc actaaattat ttcatcgctg agcaaacaat
    13861 tagagcagag cacaatgcta agttcttgaa agataggtta gatctagatg taaaaggatt
    13921 aggcgttgaa gggatattca aagtgcaaga agccttagtg aatgtttatg tcgaaaagat
    13981 gcaatagcat taacaagtaa ttattggatg ttttcaggag ggaaactatg aaattaaaag
    14041 attatatcag ggaagggtat aacattgtaa ctacaccaaa tctagcttac aaaattcaag
    14101 aagattatcc caatgtttta gtgtttactg atagagcttt agatgccatt cctataggga
    14161 agttattagt agatcgttat tatagcaaca atccagctgt tcttagtgct aagccacttc
    14221 aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag acggtagtac
    14281 caatgacagg cgagacagga agaaaagtaa ttcaacagat caaaaagagg attgctgaat
    14341 tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga gatactgact
    14401 cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata ggtcttgtcg
    14461 atattactta tcattggcat acggaagaat atgaatgcca agtaccaaaa ggagctaacg
    14521 acatcactat tgaatattat gattcacata ataacaagta cttagataga aaaatcattg
    14581 ggcacaagag agatatgtaa atgattgatg tttcaactaa acaagcaaga gctaggttct
    14641 atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat cattatgagt
    14701 gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt cttgaagtgg
    14761 atcatataaa ggagctggag tatcatccgg atttagctct agatataaac aacctaagaa
    14821 ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt ggccaacaaa
    14881 agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag atgatgtcaa
    14941 acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc tatttaagag
    15001 tcagctcatt gaagaaacaa caactgtaga ggatatatct atcaacaata gactaatagg
    15061 taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt ataccttgag
    15121 tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca gtataaagcg
    15181 aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata aaatttttgg
    15241 gaaccggtgg atggggttaa ttctgcaaat gcaacgtatt tattttaccc ctcccccctc
    15301 cgccgcccct ttagtaaata tagaaggaag gtgattggtt tgaattctaa gttggttggc
    15361 acgagaatga aacaaaaaag aattgaaaaa ggattgacac aggctgatgt agcaacgaaa
    15421 agtggtattt ctagtaggta ttatggaagt attgaaaatg ggaaaaattc ccctagtatc
    15481 gaaacgttaa actcaatttc catcgtcatt ggatgcagtt tacatttctt actcaatgat
    15541 cgcatggacg acatggaaaa acgcgtagag ctagaaaaga aacgtctgca gaagatattc
    15601 gataaaattc ctcatgacaa acttaattta gttgaagggc tgatcattca ggcagcaagg
    15661 ttacgtgtct tgttggatga taactggaag gacatcattg aaaacgggga gtacgagaaa
    15721 tttaaacaaa gcgagaacca aattgcttat gatagaaagc gtcctatagt tgaaaattat
    15781 gataatcgag ataaaactta tcaagccatc gtcaaacaac tcaccgattt attaccgcaa
    15841 ggcacaagag cggacaagaa aagtaagtta ctcggtagga aataaaaatg ctttttaacc
    15901 agcacgttga tcaatatatt cgtaaatgga aatctggtga gttaatcctt aacgaaaaaa
    15961 ggatacaact tttaaaacta atagaaaaag aaatattacc tcgcgacgac atttattact
    16021 tcgacgagga gcaaataaat aattatatcg agtttagtca agcatggtat tttgagttag
    16081 atgaatggga gaaatttatt tctgcattca tttttttgtt ttataaagaa gatgacgaag
    16141 ttgtttttga tgaatttgtt attaatatgg ggcgtggcgg gggtaaaaat ggctttattt
    16201 caacattggc aaattatttt gtcagttcgc ttcacgggat tgattactat gatgtttcaa
    16261 ttgtagctaa tagtgagaaa caagcaaaaa gaagttttca agaatgctat cgagtaatca
    16321 ataaaaaagg aaatgaagat ttaaaagaag agtttgaagc gtataaaagc agtctaaccg
    16381 gtttagagac acagtctgtt tttgagtaca aaactagtaa tgcaagctct caagatggcg
    16441 gtcgagaagg tgcagtaata tacgacgaat accacgaaat ggaaaacaca gatattgtag
    16501 atgtgttttc tggtggactg gggaaagttg atcacggacg gcaatttttc attggtacta
    16561 aggggtttgt tcgtgaaggt tactttgata ttaaatatag agagtgtgaa gatattttaa
    16621 atggtttgtc agagttcaag ggtgtatttc cgtacatatg cgaactggat gccattgaag
    16681 aaatggatga ccccaaaaat tggccgaaag caaacccggc actgcaacca ccgttgaata
    16741 aacgtgggaa acgtctgttt aacgaagtca tgaaaaagta caaaaagtta gctaaagaac
    16801 cttctggacg ctcggcattt gttactaaac gaatgaattt tcttgaagac aatatggaaa
    16861 actctgttgc ttcaagagaa gaaataatgg caacaaatcg tccatttttt gaactagata
    16921 cggtaccgat tggatctctc gatttcggta gtgttcgaga ttttgctact tgcggtttgc
    16981 tatttaaaaa tcgaaagaaa tcggaatacg cgtttaaatc atttacgttt gcaatgaaaa
    17041 atttttgcga cgttcattat ggatactcgt tgaaagagca attggttggc agtgaaaaac
    17101 gggcaccaat aaaaacttgg gaaaaaatgg ggctgatgaa agttattgac gagccatctt
    17161 taaatcctaa gtatattgtt gattggttta ttcaagctag agaagaatat ggagtgagag
    17221 taattgtcgt ggataactat aaggcagata ttttagggcc tatgcttgaa aaagaaggat
    17281 ttgaagtagt tcgactaaaa agaccatcta gtttacatcc attattagct cctcgtatag
    17341 aagatggatt tgcaaatcac aaatttattt ttggagataa tccgttaatg cgttggttta
    17401 ctaataatgt gtacgtaaag gaaacgcaag caggcaaaca attcttaaaa aaagaggaag
    17461 tcaagcgtaa aactgatggg ttccaagcat ttgtacacgc attgtacaaa gctacagaat
    17521 tagatgaaca gattgattac gatgatgctt ttgatatgtt ggatgagatt gaattttaag
    17581 gtatactttt ctttacaaag gggtgctttc atgaggataa aaggaaaaaa catatcttgg
    17641 gtattttaca tgttggggat tttagctttt acatttgtgg caataaaact atcagaaatt
    17701 attattccaa atgacagtgt agtttcaaag aatttggttt tagaaagagc taaactaatt
    17761 atttcaattt taggaccagc attaacacta attgtttttg ttcgaacaat tcaaactcaa
    17821 ttagaatctt caaaaaactt aattaaagat aacgtcagta aagattttta taatttgcta
    17881 aatttattca aaagtataca ggataaaatg aattctgaat tatttagtac aattgaaaaa
    17941 agaattaaag tacctataaa taatataaat acaaatttag atgttagaga aaataaagaa
    18001 actactaaag aagtacagga ggatttaaaa gaatatagtg catcattgaa taaagtttta
    18061 agcgaaagcg ttaaaaaaat taatgaaata tttgaggata actacaaaga cataggtagt
    18121 tatctaaaag tattccatag gattcttaaa atactaaata acaggtatga atcaaaagat
    18181 atttcgcatg aagaatacaa aatgtatgtt ggcatattac gtagtcagat tgaagattac
    18241 gaattttata taattcttat aaattctttg tttactcttc gcggaacggg tatggggatt
    18301 gagttaattg aaacatcatt ctttggtgat gaactagata ttgatttaaa acagcatttc
    18361 gaacatccag agtttttaac tgaaatcatt aaggataatt ttgttgaaaa gaaatcgtat
    18421 cgtgaatcag taaaaacaag aaaaaaaaat agaaaactat ttgaaaatag aaagataaat
    18481 gataacttat tatctctttt gagaataaga tacacgttac aagatggaaa taaaattatt
    18541 ataagtaatg gagtatagtc ctagaaatag ggctttttat tttaccttga aaggtggtga
    18601 aaatatgtga gtttatttga tatctttaag caatcaataa agaacgaaga tccatcggat
    18661 tggattcctg actttgtgtt tggtgatgaa gaatctgcta gagcatattt gaaaatcatg
    18721 gctaaaagta cagttcttga ttttgttgca agaactatgt caacgttaga agttaagttt
    18781 aaaaataaag atgggacatc agattgggat tacattctaa atgtccgacc taattctgat
    18841 atgtcagcag ctacattttg gcaaaaattt ttttatcggc tcttagatga caacgaagtg
    18901 ttagtgatat ttacagatga taatcaattg ctgattgctg atgattttag tcgaacagag
    18961 tatgccgtgt atgatgatgt ttttacaaat gtgatggtga aaaattttgt ctttgaacgt
    19021 tcatgggcca tgtctgacgt catttatatg gaatataata acgaagaatt agaaaggttt
    19081 acacgaggtt tatttgaaga ttacgccgag ttgttcggac ggattctaga agttgcaatg
    19141 cgcaataacc aaattcgtgg gtctgtctcc attgattcaa caggaacggc aaatgatgaa
    19201 aaaggaaaag atggtaagac tagatctcaa agattacaag aatatattga taagatttgg
    19261 aactctttta aaactaactc agttgcaatt gttcccaaag tgaaaggctt tgagtatgag
    19321 gaatacacca ataaacaagg cgtatcgaat caatcactag atgaactgaa taagatgaag
    19381 tcgtctttaa ttgccgatgt tgccaacgcc ataggagtac ctacggcgct tatttatggc
    19441 gaaaaagctg aactagattc aaatattaaa gcttttcgta aattgtgtat tgctccacta
    19501 gttaaaaaac ttcaggacga attggtggca aaaataatta gtcgacaaga gtacaaaaac
    19561 ggggaacgta tagaagttaa aaaagttctc caaaaagatc tattagagta tgctactcaa
    19621 attgacaaaa taacatctgg tggtgcgttt tatattgacg aagttcgtga tgaagttgat
    19681 tatgatgagt tgccaaataa tgaaggaaaa attcgtattc gtacgaaaaa ctatgaagaa
    19741 actgtgaagg gaggtgagaa cgacaatgcc gaaagctaaa aaagtaccat ttcagtttac
    19801 taacgaaatt cagaatggta agcacattct caccttgagt ggaaatgtac agaaaaaata
    19861 ttggcgtgat gatgatgtca taaacgctaa agatattcgt gactctctag atactgtaac
    19921 cgatgatatt gtaatcaagt tgaacagccc aggcggtgat gtgtttgaag ggattgagat
    19981 ttacaattat ttaaaagatc acccatcaaa tattactgta gaagttacag ggcttgccgc
    20041 aagcgcagca acgtttattg tcgctggtgc caatgatgtg attatgaatg ttgggacttc
    20101 tttgatgatt catgaagctt ctacatttgc ttgggggaat aaacaggaca tccaaaaaac
    20161 gttaaacgca ttggaaacga tcgatgattc tatcttagct atttattcag ataagactgg
    20221 ccaatctgtt gataagttgc gtgaatggat gaatgaagaa aaatggttta cagcagatga
    20281 agctgtcaaa tatggatttg ccaattctgt gaagcgtgaa gaaccacaaa acgaacctca
    20341 aaatattgca gcgatgattc aagatgctgt tgccgttgca atagcgaatt taaaggaacc
    20401 tgttactaat aaaatcgaac aagaatcaaa acaaaaatca ttaatcgcac gattgcgaaa
    20461 aggagaataa agatatgtta aaaattacag ataaaactgc agatgcaaaa aaggcattta
    20521 atgctatttc ttcgaaagaa gatgcaacac ccgaacaaat taatgctgct ctagaagaat
    20581 atgtaactgc cattgcagaa gatgccggca aacaggtgcg tgctgaatat gaagaattga
    20641 aaaatgtgac ggacaaccgt gtattagagg ctcgtggaat tcctacacta acagtagaag
    20701 aaacaaagtt ttataatgaa gccgtaaaat ctggaggttt cgattctgac gtagtttggc
    20761 cagaaacgat tttggaacgt gtttttgaaa atcttcaaaa cgatcatcct attttaggcc
    20821 tcattaactt tacaccaact gtaggtcgtg tcaaagtaat tagagcacgt cgtaaaggtg
    20881 ttgctgtttt tggaccattg cataaagacc ttgaaggcca actagatgct gaatttggtg
    20941 caactgaatt tgttcaactt gcgttgaccg catttttctt aatttctaat gataccttag
    21001 atttaggacc acgatggatt gaccgtttta tcaatctttc tttaagtgaa gcagtacgtg
    21061 atgtttgggc taaaaaaatt attacaagtt ctggtaaaga tgagccaatt ggattgctta
    21121 aagatctaga tggtgcggtc acaagtggtg tatatccaga taaagctagc gcagggacat
    21181 taacttttgc gaaagagaag attgttcttg aacttgcagg ggtgatgaaa aaattatcta
    21241 aatacacata caaagtagat ggtgacgatg aaggtgaagt ggaataccgt tctgttgctg
    21301 gacgagtgta tctaattgtt aatcctgttg attactggga tatcgtagct agagttacat
    21361 ttgcaaattt aaacaatgtg tatgggacta cgatgccatt tatcgctgtt gatcatatca
    21421 tcgaatctgt agacgtacca gaaagtaaat gcattgcata cgttgataaa gagtatgaag
    21481 ctactcaatc aagagctgaa aaagtctatg tatataaaga aacttttgca atgaaacgcg
    21541 caactttgta tgctgtcgac atgcttggta atggctatcc aacaaataat gatgcagcac
    21601 aagtatatga cttggacttt acagcggggg agtaacgtcc cctgcaattg gtgagataac
    21661 accgacgact gatggggcag tcattaatct ttcgtaaatg gggttagagt atgaacactg
    21721 aagaatttat aaaagaatat aaggcccgat ttcgtatttt ccatacttcc gaagatgagg
    21781 atatcgggaa acagttagag agcgggtttg ccgatataaa atctctaatc ggcagttttg
    21841 atccttccac ttatgagaaa ggaaaagaat tagtttatga acgtactcgg tatctacgga
    21901 acgaatcact agagtacttt tacgacaatt ttcaaacaat gattatggat gcatcaattg
    21961 atctggcagg tgctggatat gccaattaaa tcaaattata agaagcctaa aattgtagct
    22021 ggtgatctaa atacgccagt tacttttttt gagtttcaac ctaataaggg tccagaacct
    22081 ggtgatgaag aaaaaaagga actgtatttt tgtacagcac tagtttacaa cccatcaatg
    22141 aaagatcgtg acattcttgg cacaaaagaa ggcgttacga taaaaatacg ggatccccat
    22201 caatcttata ttcctactaa caaacataaa gtaaggatat tagattatcg atatggagat
    22261 aaagtttggg aaataatcga tgtctcaccg gatttggaag aaaattcaat tgtaaagata
    22321 gtattagaat taattactta aatttggtga taaacgatga gtaacgtaaa gtttgaaggc
    22381 gttcaagaaa caatccaagc tttagaaaaa aagtttggag aaaagaaaac taagacatta
    22441 acaaaaaaag caatcaatgt tggcgctgaa aaagtagaaa aacagttgca aacggacatg
    22501 accgttttta aagacaaagg ttatacgatt gatgaggttg tacgtaagaa cgctacgtat
    22561 agaaattaca aggcagaagc tgaaatcggt tggaatggtc ctcatcagcg ttatagaatc
    22621 attcacttaa atgaatgggg atatacacga aaaggtaagc aaattaggcc aagaggtttt
    22681 ggcgtaatca ctaaatcgct taaaaattcc gagccactat attttgatgc tgttggggag
    22741 gaggttaaaa agagattatg aaagatatgc tcaatatcat ttatgaggct ctttgtttga
    22801 atgagtatat tcgttcaatg acatataacg agaataccga gcaatatcgt attaaatttt
    22861 atgagcaacc tgaaaccgcc gataaaacag ggccaatgat cacacttcgt cctgtagata
    22921 cacctaacga agcatatcac ggaagtaaca aagagctttc tgtagagcat atggtacaga
    22981 ttgatgttga atcgaaatat agagcaacat gcaagcagat tcaatatgag ataaaaaaag
    23041 agatgaaaaa actaggcttt ggccaaattt ctggtcaagg gttagatgaa tacttttctg
    23101 aaacaaaacg ctttgtagac gcgcgtcgtt acgaaggaaa tacaaaaatt tacgataccg
    23161 aatattagaa attaagacac gaaaataacg tgtctttttt tattacccaa aattaggagg
    23221 aaaattttat gactttagta ggattcaaaa aaatgacaat cgggatcttt gacagcacag
    23281 ggaagattcc agcagcaaat ttatatgtaa ttgaaggtga acaagacaaa ggggcgactg
    23341 tatctgctga aatcagtggg ctatcaaaag aaccatcaaa agtttacgga tcgaacatcg
    23401 cttactatgt ctctcaaaaa ggaacaggcg atgtttcagc tacttttgga ttgttagatt
    23461 taccaacaga agtaaatgac aaaattcttg gttacaaaac cgatacaaac aaaatcagct
    23521 tcttgggaga agatactgag ccaccatatt gtgcaatttt gatggaatcg gaggatttga
    23581 acggcgatac agcaatgctt gcgatgttca aaggaaaatt cagtcgtgaa tctattaatc
    23641 ttaacactac gacaaatgaa gctttcgaac cagaagcaga agaatatgta ttttcagcga
    23701 ttgccaatga tgttgaaggt gatgctaaag gccaaacagt cgcaaaatat gttggagacg
    23761 aagaagcttc tattactgct ttgaaagaga tggtatttcc tgcagcgggg gagtaactag
    23821 ctccgttgtt ggagcaatca ctcccactat taatggggta acagttgaat taagttagga
    23881 ggataactat gccagataca tttaaaatct ataaaaaaaa tggtactgaa tttacaaaag
    23941 tcgcagaggg tgaaagtcca ctttctatta ctggaattgc agcaaatact caagttgcta
    24001 aaggagacta ccaagctacg cgtgtggttg gaggtcaaga atcagtaaaa gtagatattc
    24061 ctgcatttac cactcttcca atttcagtta ctggtgtgac gttggataaa acgacagctg
    24121 aagtcgaaca aggcggaacc ttaaaattaa ctcctactgt aacaccagca aatgcaacag
    24181 acaaaacagg aagctggagc agttcgaata cagctgtagc tactgttact ggaggcaacg
    24241 ttacagtaaa aacagacgct gaagttggcg gaactacaga agtttcattt actactacgg
    24301 atggcggtaa agtagcaaaa tgtactatta cagttattga aaaagcagcg ggctagtctt
    24361 cggactagct tttttatttg gaaaataatg gaggaatcta aatggcaaaa gtaagaattg
    24421 aattgaaaga agaatcagga aagaaagtct atgaaaacat agacacaaca ggtaaagatt
    24481 atcgaaaagc tttagaaaca atcaaaaagc taaatgaaga tggagtaatg atttggaatc
    24541 gtttagacat ttatttggat tttgctgtag gtatcttcag ggatagcaaa ttgacagctg
    24601 aacaaattct tgatggctta ccatctgaga aggtaatgag cacattggac gacatcctag
    24661 gagaagtaat ggggattgaa agtaatccag acccagacgc aaaaaagtaa cacccgaaga
    24721 agcagaagaa atgtacttaa acctatgcag agagttagtg aaagcgggat ggtcactatc
    24781 tgacatagaa gataattcct ttgaaatgat gatgaaaatt gcgtgcactt cgcctaagaa
    24841 agagaaacaa aaagaagttg atcttaaaga cttcttgaaa tccatgtaga gaggaggtaa
    24901 acatatggca aatggaaaac ctttaggcaa tatgattata aaactagact tagacagttc
    24961 agctttttca aaaggtctag ccggagctaa aaacgctgtt aaccatcaaa tgaaagcaat
    25021 gaaatcacag atgcaagtaa tgaactcttc agggaattct ctcggtgctt tacaagcaaa
    25081 atataatggt ttaggcggag ttctacaagc aaatgaaaag caagtagaat tgctcactaa
    25141 ggcttataag gatagttttg actctaatgg aaacgctact gcttcgactg ctaaatacgc
    25201 taatcaattg aatcaagcaa cagctagatc agcaagttac gaatctcaga tgaaaacaac
    25261 cgttggacag attgctcgga tgaaagtgga aacggaaggg attacgggga aactaaaagc
    25321 taattctgat gtattgatta attctgggaa aaaaattgaa gcctttggta aaggagtttc
    25381 tgggatagga tctgccctaa ctgttggagt aacagctcca atagtggcag gtgcaactgc
    25441 tgttactagt gcagctattt cttgggaaag tgcttttgca ggagtaaaaa agacaaatga
    25501 tgaagttgta gattcgaatg ggaacgtagt ttactcctac agtgatttag aaaaaggcct
    25561 tcgtgattta gcgactaaat tgccagcaag ccaccaagaa atagcgaacg ttgctgaagc
    25621 agcaggtcaa ttagggatta agacagaaaa tgtagttagt ttcaccaaga caatgattga
    25681 tatgggcgaa tctactaata tgtcagcaga aacagctgca acttctttag cacgatttgc
    25741 taatataacc caaatgtctc aaaaagactt tgataaatta ggatcagtca ttgttgatct
    25801 aggtaacaat tttgctacaa ccgaatcgga aatcactgaa atgggactgc ggttagcggg
    25861 tgctggtaaa cagattggaa tgagccaagg cgaaattcta ggatttgcta ctgctctatc
    25921 ctctgttggt gtcgaagctg aagcaggtgg gtctgccttt tcgaaggtca tgatccagat
    25981 gcaactagct gtagaaaaag gatttggcgc ttttgatcag ctgaaacaga tggcagaaga
    26041 acaaggtgtt ccgtgggtta atttagtaaa tgctgtgaga gacggtggaa aatcgttaaa
    26101 agcagtatca gagcaaatgg gattcacttc atctgattta aagaaaatgt ataaagaagc
    26161 ggacaattcg aaaagttcgc ttgaaaattt tgcgaatgtg gcaggtgtta caagtgaaca
    26221 attccagaag ttgttcaaga gtgatccttc agaagcaatc atcaagttca ttcaaggact
    26281 gaaagattca gaaaaacatg ggacttcagc aatcaaagtt ctggatgaca tggatataaa
    26341 agaagttcga ctacgcgaca gtttattgag agctgcaaat gctagtgaca tattcaaagg
    26401 agcaattgaa aaaggaaacg gcgcgtggaa acaaaataca gctttaacaa atgaagctaa
    26461 taaaagatac gaaacaactg aatcaaagct aaagatgttg aaaaacgaag tgattgatac
    26521 agcaatcgat cttggaggtc catttgtaga cgcgttgcgt gatggtctaa atgtatcaaa
    26581 accgttgata aaaaacttag ggaatatggc gaaagctttc tcagccatga gtgatgatca
    26641 acaaagaaac attatcaaat gggttggatt agcagcagca gctggtccag cgctgaagct
    26701 tttaggaact ggtacttcag taataggtaa agttacttca actactggaa gattgacgaa
    26761 aagccttgtt gatttagcag cgaaagcagc agaaaagaag gctctggatg cctttgcatc
    26821 gtcggtaaca acagctggtg ctgcagctgc aaagacagca ggagctggag gtattggcgg
    26881 gttagtccct atgatgggaa aaacagctag tgctgcaagt actgctgcgg gcgctagcgg
    26941 aataggcgca atgacaacat cactaggttt gttaggtcct gctctacttg gtattgttgg
    27001 cgttggtggc gcactagcag ttggctatgg agcatggaaa ttatttggtg aagaagcttg
    27061 gaattcctcg caaagagtaa agcaatgggg aacagatgtc ggtgaggaaa tagataatac
    27121 tcttgacgga gtacagaaga atattgaagg tgcaaatggt caattcggtc ttctcaaaga
    27181 gggttttact gttgatgatg cgaataatat ggcggctaat ttcgcaaccg ctggaacatc
    27241 tttggaaaca tctttaacag gtagaataaa ggcattagac gatgcactaa aagggctacc
    27301 tcaatcagtt caggatgcca tgaaagaagt agctgaatca gaagaagaaa ccttagggca
    27361 atctctttct aaaataaaag acaacaatga agaaataaaa aaaatcagag aaagagccgc
    27421 aaatcagaac agagacttga ctgtttcaga actacaacag atacaggatc tgatgaaagg
    27481 aacttctcaa gcgtatgtgg atacgctcga tgtttctgct aaagacagaa aaaagatact
    27541 atcagccatg aatggtgatg ttgcacaggc tacagaagaa gaagcgaaga cgtggttaca
    27601 atctttagcg aaacaaaaag ctgcaactac cgaaaattat agacaaatgc ttgaagaaaa
    27661 aaaggcggct ttaaaggaaa agggagtaag caaagaattc attgatgcat tagaagatga
    27721 aatgaatgat tacatcaaaa ctacaaatca aggattcgat agccaaatag ctgctataac
    27781 aaataaatat ccacaattga ttaacgaagt gagtttatca aatggtcaat tatttagcaa
    27841 aacggcagct gctatggaca atactggtca gtatacaaaa gtttttctcg aaagtaatga
    27901 aaagatttta gcccaagcga agaaaacatc ggatgaaata gcagataatg ccgaaaaaaa
    27961 tgcagaacaa cttggtatgg tagctgatga ggctacgcat gctggatcaa cttggaatgg
    28021 attagtttta gacgaaaaaa caggaaaagt aaaaactaac gtacaagaag tagtgaatga
    28081 agcaacgaag gatttcacta aatggaatga acttaaacct gttgttcatg atgcgaaact
    28141 aacaacgaac gcaaaagatg tcatttccat agcggcagta caaaatggtt attgggatga
    28201 tatggaatgg aaagaaaaag aagcattatt agaagatgag tgttctaaaa atgtatacaa
    28261 ggctttagaa aatgctggta aatggaatga gttagatatt ccttctaaag aagcaatcct
    28321 aacttcaaat actcctgaag ttatgggtga aactctcatg aatttgggac tatgggatac
    28381 ttacaaactt caagtgaaag atttagattt gaatgcgtat aaagtatacg atgctatcga
    28441 tggatcagaa gaaaaattaa atagttgggc tcaattagat aaccccacaa aagaattgtt
    28501 attagataac tcagattttt ctacaaaact ttttaattcc gaagaaatgt ttaaacgttg
    28561 gaatcagtta tcatctaatg caaagtttct ttcagctaat aatactaaat ttacagaggt
    28621 tcttttatca tctgacagaa tgtggaatca atgggtaaac cttcctgata atgagaaaag
    28681 attacttgca gataacgaag atttagctac cactgtttta tcttcagaag aaatatacgg
    28741 aagatggact caattaccag ataatcaaaa atatatgtta gctgacaata ctgacttagc
    28801 aaacaaaata tttgcttcag aagaatcatg gaatgcatgg aaagcaattc cagacatagt
    28861 taaaaacttg cttggtaata atattgattt actggctaaa gtgaaagatg ggactgtaag
    28921 tgtagaagaa tataacaaag ttatgccact tcttaaaact ctatatggag ataacgttaa
    28981 tttaaaagag gcaatcagtg gggctaaaag ctctatagag aattacaaca aaaatatttt
    29041 tcctaatgat aaaactgcag taggacacga tgaggcttca caagctgcta aagatgcatt
    29101 tgaagcgttc aacatcttcc aaacaaaaat accagataaa attactaaaa ctgtatcagc
    29161 tgactttata ggtcctatgc ctaatgctaa aggaacgaac ttccatcctg gcggagcagc
    29221 tatggtaaac gatcaaaaag gacctactta tgaagagctt ataaccttgc caagcggaga
    29281 agcttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt cgaaagtact
    29341 gaacgccact aaaactaaac gtctagtacc taaatatgct gatggtatag gaaacataac
    29401 gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg agctaacgac
    29461 ggtattgaga actcaactga tggcagaaac gactaatgct aaagatacta aactatcaga
    29521 tgaaacaaaa gttgccgatc cgattattcc gggcagtctt tcagaaaaat cagatcagta
    29581 tctaggaatt ggagctcaat ggttaaccaa tctaatgaat ggttggaact cagcggttcc
    29641 tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc taaaacttca
    29701 aaacaatcct aattatacgc aaggagtagc atggaataag aacttactga atggatggaa
    29761 tagtttaact gggacattta tttctacgat caattcattt tgtaatcaag caatggtcac
    29821 gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc aaaataatct
    29881 taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc ttggtaacga
    29941 ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga cattcttgtt
    30001 gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacaa caatgaacaa
    30061 tgtcgctaat acaatggttg gtggtatggg caaaggggtt aatggcgtca tatcgggagt
    30121 taactatgtg cttaaggaag tcgagtcaag taagaatatc ggaaactggc caattccaca
    30181 atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg atcaaaaagg
    30241 cctagttcac gaagaatatg tacagatgcc agatggacgt ggttttattg ccaaaggtag
    30301 agaccttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt taacaaagaa
    30361 attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt tagatattgt
    30421 tgatttgtta gatgatgaaa aaagaatgtt agaattcttg actagcaaag ttgacttttc
    30481 aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa tgtctaaagc
    30541 tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca attttgatgg
    30601 tgcagtaaat gccaatggtg tttatcaata tttagttgat gttgcacaga aagtaatggg
    30661 taagttcccc ggacttacag taacttctgg atatcgagca ggagatgctt actatcacgg
    30721 aaaacggcaa gctatagact tggcttatcc aggtatatct ggagatccga gatatacagc
    30781 agcagctaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca ttacgaatgg
    30841 acgtgtacgt gaccgcatgg gattatctgg aacagggtca agcggacaat ggacgaattg
    30901 gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt ctggcgatat
    30961 attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg tgtctaaagc
    31021 attgaaaatg aatggattac cagctacagc agcttatata aatgcctgga tgtcccaaat
    31081 ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc tcgctgaagg
    31141 caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg cttttcctgg
    31201 tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt atgctaagaa
    31261 acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat atgccaatgg
    31321 tggattgatt accaaagacg gactatatcg agcgggagaa ggaaataaac cagaaatggt
    31381 tattccattg acaagaaaaa caagagctat tgaattgatg ggtcaagcat tagcttttct
    31441 ttctggcgat gacaagaaac gctccaacac tacaaataca gctgataatt cagcagagct
    31501 tgtaactttg attaaacagc aacaaaaaca gcataatgag ttaatgatga ttcttagagc
    31561 gattcttgga aaagatttaa gtctcaagtc ttctgacatt gggcaagctg ctaatcatta
    31621 tatgggctct gatttgaata aacttcgata tacgaatgga ggtgtttgat aattgtttta
    31681 caaattacag ttcaatcaaa atgggaagtt gtttgatccg caaagaaaag aaaaaattgt
    31741 atgtaaagaa atcaaacgtc aagcaccagt gtatgaagtg aattacgaag attttgaagg
    31801 gacgaacggt agcagagaag ctaatgctag ttttcgtcct tttgaattag tgctgacttt
    31861 tgatatcttt tacaaaaata agcatgataa agaactgcta ttaacagagt tctatgagct
    31921 aatttttgtc ggttatcaat attacatttc ctatgattta agcccaggaa aaagatttaa
    31981 agtaaatcca acaaatttcg aaattactga ggaagagaac gattattcta cgatagaaat
    32041 aacctttagt attccatcag ggagtgctga atccagcgca accactttaa cggatttcaa
    32101 tctagaggaa gaatggcaat tctcgcaagg tctagtggcg gaagattaca agtatacgca
    32161 tcagactagt cacttcatta tttacaatgc tggaagcttt gaaattgatc cacgtgaaca
    32221 ttatctacgg atcgcattag aaggagaatc agaaggaaat gtgacgattt tcaataaaac
    32281 aactggcgat cgatttattt actatccatc actttctacg aatctcggac agacattagt
    32341 cttggatggc gtgattccaa aattgaacgg tgtaagttgt ggaatcaata cgaatcacgg
    32401 tctgatcaat ttggttgagg gagtcaatga aatcgagatc caaaatatta ctcgcgtgaa
    32461 atcttcatgg gatttccgtt tcttatataa gtaggtgatt gagtgactga gttaattatt
    32521 cgaaattatg aacaaaccaa agaagaaatc cttgtcggtt atgacaaggg ttctttttat
    32581 gaaaactggc aacaaaatga aacgtgggag attggcttta ccgttaccag caattcgttg
    32641 aatcaagaag tatttgattt agtcgaatac gagtcttctg ttttctacaa cggacaggaa
    32701 tttgtgatca aagaaatgac tcgcaaagca cttggacaat tgctgacgaa acaagtagtt
    32761 gcgacacata tctattacac cgttcaagat ggttatcagt acaacacagt gacaggtgcg
    32821 aaatctatta gccagttact cacgcatgta tttagtgcag ggagtcgcgg ctttacatgg
    32881 gaagtcattg atccaaacaa aaaattcctt accgttgaac aagaaaattt cggtaacgcg
    32941 aattacttga agctgatcaa tgagattcta tctgactata atgcagtcgt gattccgaat
    33001 aataaacatc taactttcta tcctgccagt gagtacggcc agcagacgga agaacagatt
    33061 cgctataaat acaatacaga tgaagtgtcg tttgatattg atacgtattc cttgaagacg
    33121 cagatcaaag gctatggaaa attgaaggat ggtgcaaata ctgagaatcc taaagatagt
    33181 gactatatat ttactcccat cacttataca agccctgaat cacagaagtg gggaatcagg
    33241 atacaagatc ctgttaaaga cgaacggtat accgtatcag ggaacatgct cgagcggtta
    33301 aagacagact tgcaagacta tccaaatatt tcgggatccg taaccttgaa atggaaaatc
    33361 agtcccaaca aaggcgatca cgtcccattc atttatgaac ctttgaatat taatacgtac
    33421 attcaagtgg taggaatcaa gacgtatcca gcgataccaa ataagccacc agaaatcaca
    33481 ttgagcaaca caaagaaaac aatgacgtcg atactcgctg aaatggcgaa gaaaggagtg
    33541 atttgatggg gttattaaaa ttaatcagta accgtatctc tacggaatgg aaagagaaat
    33601 ttaataaaaa cattgactac cttaatgatc ttgaaaagaa attgtctgat caagacaaat
    33661 caacaaacag tcgtattgat aatctcgtgc ttcattcagg aggtgattct cctaacgaag
    33721 tagtggatgc tcgggtgaat cacaaaggcg aaacattcgc tacgttacaa ggaagactga
    33781 cagacaccga aaaaaaagtt tctgaaagtg tctctaccct agaaaaaaat caagctgata
    33841 taaaagcaca agtcgctcaa ttaaacaata gtgtagaaac aatcattggt ggtagtgctg
    33901 actggataaa tctttatgtt tcagctgatt tgggaagcga ccaaattgga gatggatcag
    33961 aagaaaaacc atttgcgaca attcagatgg ctgtaaatca gatcccgtta gtttctattc
    34021 ctggaattag aatttgggta gatgatggtg tttatttgga agatgttttc ataagaaatg
    34081 ttagtgctac tactattcat attggcccta aaaatgatac atctgtaatt gatccatcaa
    34141 aatcagatat gccagtaaaa cttcgcagtc ttgcatttta tcaatgtaaa ggttttttca
    34201 aggtcacagg tcttcaattt gtagatacta taaatgcacc caaaaatagt ggattaatat
    34261 atagtctgat gttgctacaa ggaggatatc tttctgtcga taagtgtaaa tttgctgagg
    34321 ataacagaaa tttaaccagc gcagcgattt atacagaagg gttgtctgct tctaacgtct
    34381 acaatacttg ctatttttat agacaaaata ttgtagttta tgccaattta atgtcacaag
    34441 ttcttatatc acaacaaaca agcggcaaag aaaacacaac aggtgctagg agtaaggatg
    34501 caatcattcg tgggaaattc tcagtgggtt ttgctgataa aaatgaagat gttaaagatc
    34561 ttggcttaat cattactaaa gggacggtgt tgtcctgatg gcgtttaaaa ttaatgaatc
    34621 gattattgta attcaagcag aagccactag tccaaacagg acgaatgttg ttttttggtc
    34681 gcatgatcga ggaacagcta agcttcgaat gaagttagtt cggaaaaacg gcatccctca
    34741 aagcttaccc gaagggacaa ctgttccgat tcgtctgatg ttcagatctg caacggcaga
    34801 aggtggttat ggaaaacatg actatctcgc caccattgaa gatcgtgtaa atggtattgt
    34861 gtccattgtg ctggaagata atattttagg ctatgttgga attgtcgaag gtagtgttta
    34921 tattgatttc ccaaacgacc gctcgttaga tacagctggt cgttttactt ttgacatcaa
    34981 acgcagtcca atcgatgata gtacaccaga attggaagat tattatttca atggtttcag
    35041 tcagactatt gataaaatcg aaaaaattct agctgatgga aagcaagaga ttgaacagaa
    35101 aattacggaa tctgaaacgc agattgatgc gaaattaaaa aatacaaacg acaaaatcac
    35161 gaaagccaat caagatgtca caactctcaa tacgaatatt gataaagcaa atgatcgtat
    35221 tgatcaaacg aaccagcaaa taagtgatct cggaaagctg aaaaagatgt actccaacag
    35281 catcgatttc gggaactatg attatagtgg aaatcctaat ttgttaagta agctatcata
    35341 cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaaaggtg aaaactcgtt
    35401 taaatataat aagatatcag ctgaagtgga aggtggagta gagctatatt ataaacgaag
    35461 aggtatagct aactggctac catctaataa aacgcttgta atgactgtta agcttagagc
    35521 tggagcggac tatagtccag ttgacggaaa acttatactg attagatata ggtatgttga
    35581 cagtggaact ggcaagattg ttttagactt acctattaac agtaattcga taactcagga
    35641 atggaaagag tttagtatta ctggaactac tccaacattt agcccacaag cataccatcc
    35701 ttggatacaa tttagggctc aagatgggat acttggggaa atagaaatga gctatgacat
    35761 caaaatcgaa gaaggctcaa cagctacacc attccaacct aacttattag cagaacctta
    35821 caacatgtgt cgcgaatatc ctaacgaaaa tattgccgat catacagtta agttcccaat
    35881 cgaatctggc gaccaccaaa tatatcaagg ttacacagaa gaagagctta tgataggtca
    35941 aacgtatact atcacgctta aaggaacaaa acccgcaagt caaacctttg tagcgtataa
    36001 tcattggact gctcgtttag gagaactaaa gccggttgat gggttgacag acgtatggtc
    36061 tctaacattc acaccaacga atgttgtggc gatgcctaaa cttttccgtg tttatcagta
    36121 tccacgatca acagtaggcg catgccaaat tgactggctc aagattgaaa aaggcaacac
    36181 ccgaacgccc aatattagtc agtttaaata cttcggtgaa ggcttgaaag atagtaacaa
    36241 tccgaacgac tacagctggg atatcacacc tgaatatgct gaaaaaggct tgaataatac
    36301 ggttagtcta actgaacccg aaaccgtttt aggacttaaa aatttcaaag atggggtcca
    36361 gatcgacgga gtagatgtgg caacagttaa aaacttaggt agtgtgttac agagtggatc
    36421 tggtttgact actattcaat cagataagca aagcgcattt gatgaatttt catacgaatt
    36481 cgaacgtatt ggtgataggg tattctttca cgcacgagta aaaacaaact cgacaacagc
    36541 cgtagcttca catgtgaatc tactagaaat tcctttagga tttcagtttc ctattggatg
    36601 gacaattgta ggggttccgt tgtcggttgt acagtggact acgccacaag gagaaatcag
    36661 cgctttggta atcactgatt caactggtaa acaagtagtt aaatttgcaa ctaatcgagt
    36721 aggaaatcat tatatttcag gagaatggcg aacagctgat agttatccag aaacaaagta
    36781 ggagggaaat aaatgaaaaa catttggaaa tacggacgta ctggcggaga gtatgcagga
    36841 aaagtattgg acgatatgct tgtatccgtt ccttacacgg atcagccacc gctcgaagga
    36901 ttacgtgctg atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaactgaac
    36961 caatggattg ttttagcgaa cacactggat cacaacgatt taaacaatct caaagcgatg
    37021 tacgaggcac tggaacacga gaacggcgat ttaaaacagc tcaacgccaa actcatgcta
    37081 agcgatgtag caattaaaca ggaaaatact gcattgaaag aaaaagcgga tagtttagca
    37141 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
    37201 aaagagcaac taaatccaac tttaaaggga ggtgagtagt atgtttagtt ttagcgatgt
    37261 gaaaatgatg tatgattggg gctgttttac tgacgatcaa gttcgactat tcgttccact
    37321 atgcattaca gacgaagaag cagataaaat cattagcaaa gaagagagcg catcttaagt
    37381 gatgcgtttt tttgttggaa agttggtgga acatgaaaga agaagcgctc caagacgttg
    37441 tggagagatt agtaagaatt gaaacaaaat tagacaacta cgaatcactt agagaaaagg
    37501 ctgatagtgc aaaagattta gcagataaag cctattcagt agcactaaac aatgcagaag
    37561 acatcaagga aatgaagaac aataataaat gggcttgggg ctatatgatt ggcttaggca
    37621 ttacgatcat tggctatttc ttgactaaat tgtaaaggag gtgagaagaa atgattttac
    37681 cagataagta ttatcaagtc attaaatgga cagttttaac agtattgcca gctgcatctg
    37741 ctttagtagc catgttaggg aaagcgtatg gatggaatgg aacagatatg atagtgttga
    37801 ctatcaatgc agtagcaaca tttttaggcg ttatcactgg tgtgtcggct tataatttga
    37861 aaaaatagga ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctattaacg
    37921 gctctttttt tattgccaat taatgggttt gcctatacta tcaacaatga atttaatttg
    37981 ggcgcaaatg aaggtagctc acaaatagca aataatcagt a
//
LOCUS       DAZKCN010000013        37102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000013 DAZKCN010000000
VERSION     DAZKCN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.013"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..444)
                     /locus_tag="ACG8K3_000669"
     CDS             complement(160..444)
                     /locus_tag="ACG8K3_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144572.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="ACG8K3_000670"
     CDS             complement(462..1352)
                     /locus_tag="ACG8K3_000670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9144573.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="ACG8K3_000671"
     CDS             complement(1372..1590)
                     /locus_tag="ACG8K3_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP9144574.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="ACG8K3_000672"
     CDS             complement(1560..3260)
                     /locus_tag="ACG8K3_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9144575.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="ACG8K3_000673"
     CDS             3527..4300
                     /locus_tag="ACG8K3_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144576.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="ACG8K3_000674"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="ACG8K3_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP9144577.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="ACG8K3_000675"
     CDS             complement(5124..6095)
                     /locus_tag="ACG8K3_000675"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9144578.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="ACG8K3_000676"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="ACG8K3_000676"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9144579.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="ACG8K3_000677"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="ACG8K3_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP9144580.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="ACG8K3_000678"
     CDS             complement(9050..9874)
                     /locus_tag="ACG8K3_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP9144581.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="ACG8K3_000679"
     CDS             complement(9847..10527)
                     /locus_tag="ACG8K3_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9144582.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="ACG8K3_000680"
     CDS             complement(10550..11488)
                     /locus_tag="ACG8K3_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9144583.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /gene="ispE"
                     /locus_tag="ACG8K3_000681"
     CDS             complement(11733..12584)
                     /gene="ispE"
                     /locus_tag="ACG8K3_000681"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9144584.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="ACG8K3_000682"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="ACG8K3_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP9144585.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="ACG8K3_000683"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="ACG8K3_000683"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9144586.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="ACG8K3_000684"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="ACG8K3_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP9144587.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="ACG8K3_000685"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="ACG8K3_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9144588.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="ACG8K3_000686"
     CDS             complement(19462..20703)
                     /locus_tag="ACG8K3_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP9144589.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="ACG8K3_000687"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="ACG8K3_000687"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9144590.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22653)
                     /locus_tag="ACG8K3_000688"
     CDS             complement(21730..22653)
                     /locus_tag="ACG8K3_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP9144591.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="ACG8K3_000689"
     CDS             complement(23392..24678)
                     /locus_tag="ACG8K3_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9144592.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="ACG8K3_000690"
     CDS             complement(24671..25939)
                     /locus_tag="ACG8K3_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9144593.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="ACG8K3_000691"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="ACG8K3_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9144594.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="ACG8K3_000692"
     CDS             complement(26711..27157)
                     /locus_tag="ACG8K3_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144595.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="ACG8K3_000693"
     CDS             complement(27250..27495)
                     /locus_tag="ACG8K3_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP9144596.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="ACG8K3_000694"
     CDS             complement(27610..28188)
                     /locus_tag="ACG8K3_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9144597.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="ACG8K3_000695"
     CDS             complement(28265..28795)
                     /locus_tag="ACG8K3_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP9144598.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="ACG8K3_000696"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="ACG8K3_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9144599.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="ACG8K3_000697"
     CDS             complement(29652..30047)
                     /locus_tag="ACG8K3_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP9144600.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /gene="cysS"
                     /locus_tag="ACG8K3_000698"
     CDS             complement(30044..31456)
                     /gene="cysS"
                     /locus_tag="ACG8K3_000698"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9144601.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="epsC"
                     /locus_tag="ACG8K3_000699"
     CDS             complement(31453..31989)
                     /gene="epsC"
                     /locus_tag="ACG8K3_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP9144602.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(32075..32314)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32311..33771)
                     /gene="gltX"
                     /locus_tag="ACG8K3_000700"
     CDS             complement(32311..33771)
                     /gene="gltX"
                     /locus_tag="ACG8K3_000700"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9144603.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="ACG8K3_000701"
     CDS             complement(33941..35098)
                     /locus_tag="ACG8K3_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP9144604.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="ACG8K3_000702"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="ACG8K3_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9144605.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="ACG8K3_000703"
     CDS             complement(36565..37092)
                     /locus_tag="ACG8K3_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9144606.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        10249 a         8086 c         6507 g        12260 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ct
//
LOCUS       DAZKCN010000014        36568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000014 DAZKCN010000000
VERSION     DAZKCN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.014"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8K3_000704"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8K3_000704"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9144607.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8K3_000705"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8K3_000705"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9144608.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8K3_000706"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8K3_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9144609.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8K3_000707"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8K3_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9144610.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8K3_000708"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8K3_000708"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9144611.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8K3_000709"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8K3_000709"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9144612.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8K3_000710"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8K3_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP9144613.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8K3_000711"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8K3_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP9144614.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8K3_000712"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8K3_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP9144615.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8K3_000713"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8K3_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9144616.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8K3_000714"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8K3_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9144617.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8K3_000715"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8K3_000715"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9144618.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8K3_000716"
     CDS             15402..16376
                     /locus_tag="ACG8K3_000716"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP9144619.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8K3_000717"
     CDS             16546..17946
                     /locus_tag="ACG8K3_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP9144620.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8K3_000718"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8K3_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9144621.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8K3_000719"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8K3_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9144622.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8K3_000720"
     CDS             19454..21253
                     /locus_tag="ACG8K3_000720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP9144623.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8K3_000721"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8K3_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9144624.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8K3_000722"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8K3_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9144625.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8K3_000723"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8K3_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9144626.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8K3_000724"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8K3_000724"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP9144627.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8K3_000725"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8K3_000725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9144628.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8K3_000726"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8K3_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP9144629.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8K3_000727"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8K3_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP9144630.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8K3_000728"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8K3_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP9144631.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8K3_000729"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8K3_000729"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9144632.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8K3_000730"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8K3_000730"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9144633.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8K3_000731"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8K3_000731"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9144634.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8K3_000732"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8K3_000732"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9144635.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8K3_000733"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8K3_000733"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9144636.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8K3_000734"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8K3_000734"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9144637.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAZKCN010000015        34798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000015 DAZKCN010000000
VERSION     DAZKCN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.015"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACG8K3_000735"
     CDS             <1..565
                     /locus_tag="ACG8K3_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144638.1"
                     /translation="KFYIDSSKKTIKQISTETGINYTTLTNYYRGERNPRNTETWNIL
                     ADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNLY
                     FSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKALNLNIDERKELES
                     ELFGNLTQVQVNNLKREITARLISKLQ"
     gene            620..1786
                     /locus_tag="ACG8K3_000736"
     CDS             620..1786
                     /locus_tag="ACG8K3_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9144639.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(1875..2267)
                     /gene="rpsI"
                     /locus_tag="ACG8K3_000737"
     CDS             complement(1875..2267)
                     /gene="rpsI"
                     /locus_tag="ACG8K3_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9144640.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2283..2711)
                     /gene="rplM"
                     /locus_tag="ACG8K3_000738"
     CDS             complement(2283..2711)
                     /gene="rplM"
                     /locus_tag="ACG8K3_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9144641.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3245..3712)
                     /locus_tag="ACG8K3_000739"
     CDS             complement(3245..3712)
                     /locus_tag="ACG8K3_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9144642.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3832..5370)
                     /locus_tag="ACG8K3_000740"
     CDS             complement(3832..5370)
                     /locus_tag="ACG8K3_000740"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9144643.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5450..6136
                     /locus_tag="ACG8K3_000741"
     CDS             5450..6136
                     /locus_tag="ACG8K3_000741"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9144644.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6337..6864)
                     /gene="msrA"
                     /locus_tag="ACG8K3_000742"
     CDS             complement(6337..6864)
                     /gene="msrA"
                     /locus_tag="ACG8K3_000742"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9144645.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6948..7625)
                     /gene="pgmB"
                     /locus_tag="ACG8K3_000743"
     CDS             complement(6948..7625)
                     /gene="pgmB"
                     /locus_tag="ACG8K3_000743"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9144646.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7622..8752)
                     /locus_tag="ACG8K3_000744"
     CDS             complement(7622..8752)
                     /locus_tag="ACG8K3_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9144647.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8752..10935)
                     /locus_tag="ACG8K3_000745"
     CDS             complement(8752..10935)
                     /locus_tag="ACG8K3_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP9144648.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10932..11957)
                     /locus_tag="ACG8K3_000746"
     CDS             complement(10932..11957)
                     /locus_tag="ACG8K3_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9144649.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12002..12829)
                     /locus_tag="ACG8K3_000747"
     CDS             complement(12002..12829)
                     /locus_tag="ACG8K3_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9144650.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12826..13893)
                     /locus_tag="ACG8K3_000748"
     CDS             complement(12826..13893)
                     /locus_tag="ACG8K3_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9144651.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13919..14755)
                     /locus_tag="ACG8K3_000749"
     CDS             complement(13919..14755)
                     /locus_tag="ACG8K3_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144652.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14752..15621)
                     /locus_tag="ACG8K3_000750"
     CDS             complement(14752..15621)
                     /locus_tag="ACG8K3_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9144653.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15691..16974)
                     /locus_tag="ACG8K3_000751"
     CDS             complement(15691..16974)
                     /locus_tag="ACG8K3_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9144654.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16988..18673)
                     /locus_tag="ACG8K3_000752"
     CDS             complement(16988..18673)
                     /locus_tag="ACG8K3_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP9144655.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18948..19973
                     /locus_tag="ACG8K3_000753"
     CDS             18948..19973
                     /locus_tag="ACG8K3_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9144656.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20568..24221)
                     /gene="rpoC"
                     /locus_tag="ACG8K3_000754"
     CDS             complement(20568..24221)
                     /gene="rpoC"
                     /locus_tag="ACG8K3_000754"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9144657.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24300..27917)
                     /gene="rpoB"
                     /locus_tag="ACG8K3_000755"
     CDS             complement(24300..27917)
                     /gene="rpoB"
                     /locus_tag="ACG8K3_000755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9144658.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28645..29613
                     /locus_tag="ACG8K3_000756"
     CDS             28645..29613
                     /locus_tag="ACG8K3_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9144659.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29631..30536
                     /locus_tag="ACG8K3_000757"
     CDS             29631..30536
                     /locus_tag="ACG8K3_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144660.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30533..31339
                     /locus_tag="ACG8K3_000758"
     CDS             30533..31339
                     /locus_tag="ACG8K3_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144661.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31496..32464
                     /locus_tag="ACG8K3_000759"
     CDS             31496..32464
                     /locus_tag="ACG8K3_000759"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP9144662.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32781..33305)
                     /locus_tag="ACG8K3_000760"
     CDS             complement(32781..33305)
                     /locus_tag="ACG8K3_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP9144663.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33592..>34798
                     /gene="scm"
                     /locus_tag="ACG8K3_000761"
     CDS             33592..>34798
                     /gene="scm"
                     /locus_tag="ACG8K3_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9144664.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTT"
BASE COUNT         9961 a         7415 c         6506 g        10916 t
ORIGIN      
        1 aaaattttat attgattcca gtaaaaagac cattaaacaa attagtacag aaactggtat
       61 taactataca actcttacga actattaccg tggtgagcga aatcctagaa atactgaaac
      121 atggaatatc cttgctgatt atttccatgt tcctgtaagt tatttgatgg gattagacga
      181 aacaaattcc atagaacaac ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa
      241 ggacaaaata gtcaagaata ttgccagtct tgactcagat tattttactg aattatctaa
      301 tctatacttt tccatgactt ctttacttga agtcaaactt caagaagatg actccgactt
      361 tcttttttta aaaagtattc tagaggactc ctttgaatac acgattctat ttgccaaaca
      421 aaagaagttt attaataaag ctttaaactt aaatatcgat gaaagaaaag agttagaatc
      481 tgagttattt ggcaacttaa cccaagtaca ggttaacaat ctaaaaagag agataacagc
      541 tcgattaata tcaaaacttc aataaaacta attattcaat cagccaactc gccaagttat
      601 cagattggag agattagtta tgtcaataca aaagtacaca acaaaaagcg gtgcacgata
      661 tagtgtccgc ttgtatgttc gtaaagatga aaatggaaag caagaatact atgtaaaaaa
      721 agggtttaaa actgaaaaag aagccaagtt acacgaagca agaaaaaagg tcgagattga
      781 agataccggc tatcataagc cttctaatga cttgtttata acagtgtatg aagaatggtt
      841 gccttcttat aggaatcgtg tacaagaaac gacatatcaa cgtacaaaag accttttccg
      901 cttgcatata ctaccaaaat tcgggaataa gtccatttct aagattactc ctgttcactg
      961 tcagaatgca gtcaatgaat gggctttaaa attcaagaat atcaaacagc ttaagtctta
     1021 cagctctcaa atatttgagt atgccatttt tgcagagcta ttacaccgaa atcctatggc
     1081 aaatacaaat ctaccaaagc gggaaaagaa agaaaatgaa aattactttt cattagaaga
     1141 actaaaagct ttttgggata ttctcaataa agaagagcct ttaaaacata ttttaatctt
     1201 ccagcttttg attactactg gtatacgtaa aggagagcta gctgctcttc attggtctga
     1261 catttatttt gaagaacagc taatgtatgt tagaaagagc tatgcaacta ttcgaaatga
     1321 agagaagaat gcaagaagaa aaactgtcag gattcaaaaa aatacaaaaa atatatcttc
     1381 cgaacgaata ttaccaattg atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga
     1441 acaagctcga gagcttttgc agtttggcat aaatacaaat acaaaaaatc aacttatctt
     1501 tacctatgta aatgcagacg gtgacattaa tcaacctttg catgcggact attcaaataa
     1561 tatattgaaa cgtttagaga agaaatataa atttaagcac gttactattc atggattccg
     1621 gcacactcac gctactcttc tactagaagg tggagcaagt atcaaagaaa cacaagatcg
     1681 attgggtcat aaaaatgctg aaactaccct taacacttat tctcatgtta ctgaaaaagc
     1741 tcaacgaaat gcgttagaaa aattcattgc ttacactggt ttataatatt gtcattacct
     1801 ttctcattac cagaacgaaa aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt
     1861 tggtataaca acgattaacg ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct
     1921 ggttttttac gttcgaccat acgtgcgtca cgagtaagta agccagcacg tttaagagct
     1981 aaacggaagt caggatcaac ttctagcaat gcacgagcga taccatgacg gatagctcct
     2041 gattgtccag cgtatcctcc gccgtttacg ttaacaaata cgtcatatgc gccttttgtt
     2101 tcagtaacag cgaaaggttg attgattact tcacgcaagt cagcatgtgg aatgtattct
     2161 tcaacatctt ttttatttac agtaattttg ccagttcctg gtactagacg tacgcgagca
     2221 actgcgtttt tacgacggcc tgtgccgata tattgaactt gtgccaatga aattccctcc
     2281 tattagatta agtttgtgat gtctaaaact tctggttgtt gtgctgcatg tggatgttct
     2341 gctccaccgt atacgttcaa ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc
     2401 atacctttta cagaagtttc gatcaaacga cgagaatttt tagcacgcaa ttcgccagca
     2461 gtgattgatt tcaatcctcc tgggtagttg ctgtgacggt aataaatttt gtcagtcgct
     2521 tttttacctg ttaatttcac tttgtctgca ttgatgacga tgacgaaatc gccagtatcc
     2581 aaatgaggtg tgaaagttgg tttattttta ccacgtagta cagatgcaac aactgttgaa
     2641 agacgtccca atggaacatc agttgcgtct actacatacc atttacgttc tacttcgcct
     2701 ggtttggcca tatatgttgt acgcacgtta ttttcctcca atttaagttc tgatgtttga
     2761 catacacttc gagtttccgg ggctctttgt ggggcaaaca ataccattta acatattacc
     2821 cgctaatcta gtcaatgtca acgtttttct gcttgattga aaaatttata tgaaacttta
     2881 cttcttttct ttcttttata taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag
     2941 gctgtgacaa aagtcttgtc acaacccctt attttgaata aacggtgttc tcctgccaac
     3001 tcctcggtca caagtcacaa tatccataaa acataagaag ctgtttttta aaattgtttc
     3061 ttgtgactcg gagctgaacg gcaggcttac aacctcttgg taaacggtgt tcaatcagct
     3121 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttga cgtttgtttc
     3181 tctaatactc aggtcaaaac gctgatttca caacctcttt ttcggtcaag ttcaacaaca
     3241 gtttttacgc gtcgatctct ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt
     3301 tttctgtggc tgttttgaat tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc
     3361 cagaaacttc gattcctttt tggtaaagat ctgctagata acgatagcct gcaacaagtg
     3421 tttccaaacg ttctggaata gtacgatccc agcggccaat ttcatcaggg atttttgtgt
     3481 tatctgcaaa ttcacgcaat gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg
     3541 atacttcatc taactgagcg ttcaaatcat ccatgaactc atccatcatt gggtgcaatg
     3601 taaggaaacc tggtcctctc atgtaccaat gtgtttggtg aacaacaaca gacatttggc
     3661 ttagatctgc aactaattga tttaatactt ctttggtttg ttggaatttc atctttcttg
     3721 cctcctaaca ttttcttaca cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa
     3781 atcatttacc tgagaacgat tttcagttcg cttttttatc cgtaaagcca atcatttttc
     3841 agaatcagct aattcttctc gcagttgagc aaattgctga taagtctgtg ctaaatgctt
     3901 ttgtgtcgaa acaaataatg gatagatttt ctgatatttt ttatgatcag tttcttgtac
     3961 tccaacttgc tctttcaatt ctatcaattc ctgtgcttca ctgatacttt ggatctttcc
     4021 gatgcttctc cagcctaaaa tcacagctcc taatgcggat gcttctgtga cagtagggaa
     4081 aacaagcggg taacccagta catcagccat catctgacga aaaatcggag attgagcaaa
     4141 cccgcctgta gcacggatct ctgtgagctg attttcctgg caaactcctt ttaaaatccg
     4201 ataaaggttg agacagatcc cttctagaat cgccttcatc atttcttttt ctgtatgttg
     4261 ccgctgtaaa ccgataaaac tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc
     4321 aagtaaatag ggatgaaaaa tcagaccatt ggcacccgct ggcacaaaag cgatagccga
     4381 aagcagtgca tcatatggat tttctccttt ttctttagca gattgaacga cttcctgtaa
     4441 aaatgtatgc cctgcccaat caaagatgcc agcaccattg ctagtcgctc ctcccactac
     4501 ccaatgtttc tgatccagaa cataacaaaa cgtctccatt tctggatgga actgaggctg
     4561 agtagtaata aaacgaagtg ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc
     4621 aaaagcacct aaccctagat tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat
     4681 atcatttggc aaacctagtt ctgctgctgt ttttgatggt agacctgtta cttgataggt
     4741 ggaaggaaag acttgcggca attgctctat tcgaatgttc aagtagtcaa gcacttcttc
     4801 tgcccattgg aattcatgga tatcaaaata tccagttcct gaagctgttc cgtagtcgct
     4861 gatcagttcg ccaaacaatc tataaaagat atagtctttg ataccgacaa aataagcagt
     4921 ttttttgaat aattcaggtt ggctgttttg cagccacttg attttgttca atggtgtcat
     4981 tggatgaatc ggcgtcccag tctgccgata aaattctttg ccaagcacgg tatatttcag
     5041 tttttctgct tcgtccgctg ctcggttatc tgcccaagtg atcattcgag ataacggtgt
     5101 tttctcttca tccaacaata tcaggccatg catcgcagag gagaaggaga caaattgaat
     5161 gattgtgtct gaaggcaact gtttaattac ttcttttata acggtgcaga caccttgaaa
     5221 gatcaattcc tgatcttgtt ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa
     5281 ataagcggat gctgcttctt gggcttcatc tgtgaaagca actgctttgg tttgtgtggt
     5341 tccgatatct aatccgattg aaacgttcat tttttcacct caaattttat gtatgtacaa
     5401 aaatttagtt taagcttgcg tatcttggtt ttgaaaaggg ggagatggcg tgttacttta
     5461 ttttatcatt ggttgctgta tcggatcatt tttatgttta accgcccagc ggattccgtt
     5521 aggtcactct attatttatc ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg
     5581 gtatgaattg attccgattc tctcgatcat cgtccaacga tttcgctgcc gctattgcag
     5641 gtgtcggttg cccttctact atttgttggc agaagcctta tgtggtggtc tttttgcctg
     5701 gttctttact ttttcttccg caagaaattt ttcgacgttc atatggatgc tttcggcttt
     5761 gctattttcg ttgatggatt tattttactt catggtccat tctcacacgc tattctgttc
     5821 ttgggcaatt ttatggattt tttggttgca ggccggcgtc ttccaatggc aaagtgtgct
     5881 gttaacgttt atcgtaggcg gtgtgtgcct tcgctatggt caatcctatc tcggagctgg
     5941 cgatactctg ttgatgcttt cttggagtgg tggcttgtct ctagaagagt tgcttcagat
     6001 cttgtttctt gctagtctgt ttggaatatc cgttttttct gtttacttta tctgtcataa
     6061 aaaaaagttg gaaaagctgc ctttcattcc ttttttgagc agtgcgttgc tgattgtcct
     6121 tcatcttagg tattgaaaaa aggttgtgac aaacgacttg tcacaacctc tgatatatag
     6181 tgttctctta ccaaatcctt gtgacttgga gctaggcgac aggttcacaa cctctccact
     6241 aaatggtgtt caatcagctg accctcagta ttagctcaaa atgctggttt tacaacctct
     6301 gatatacggt gttccataag tagctttttc ggcttcttat ttcttgacgc cccaatgtcg
     6361 acgaaggaaa cctgcacgtc cagaaccttc tcgataaaga ttgtaatgga gcttgttttt
     6421 cttgtaataa tcctgatggt aatcttctgc aggataaaat tcgcttcttg gttcgatttt
     6481 tgtcacgatt ggcttatcga atcgcccgct gtcttgcaat gcttgttttg aacgctctgc
     6541 aatatttcgt tcttcttcat cattatagta gatcactgga cgataagaat caccgcgatc
     6601 agcgaactgt cctaatgcgt cagtcggatc ggtttgctgc caatagattt ccaccaactg
     6661 atcataagaa ataacttctg gatcatattc gatctctact gcttctgtat gccctgttgt
     6721 gcctgttgtt acttgttcat aagttggatt tggtacatgc ccgccagtat aaccggatgt
     6781 cacagaaatg atccctggct gctcttcaaa tggtttgacc atacaccaaa agcatccgcc
     6841 tgcgaaaact gctttttttg tcatataaaa tccccctttt ttctcatcat accttgattt
     6901 ccaaaaggag gataacgttt tgcttgtgac agaaaaaacg gaaactatta tatacgtgat
     6961 acggcttctg ataaatgcca aaacgaacgt acttgatcaa aaacggttaa atcaggaatc
     7021 acatattcag cagaaggaag atttatgttt tcacctattc ccaatacatg catgttacca
     7081 gccaaagctg cttgacaacc agcttctgaa tcttcaatga ccaagcatgc atttggcggt
     7141 acattcaatt gctgtgctcc tttgagaaaa acttctggat caggttttgc ttttgatact
     7201 tgcgtgccgt caataaggac atcaaaataa cttgtcaaat tcgttttttc taaaatgagg
     7261 cgtgcatttt tgcttgctga tcccaatccg atcttgatgt ttgttttttt caagtactca
     7321 agaacagcta ttgcaccagg caaaacggat gtttcatcca ttttttgaat cgcttgaaga
     7381 tatagattgt ttttttgctc agcaaaggct gatttctctt tttctgagaa tttctgttcc
     7441 atttttccaa atgacagcaa tcgctctagt gactcggttc tggatattcc tttcagttgc
     7501 tcgttttctt tctcagtgaa tggaatggac agctggttag ctaaaaccag ccacgcttcg
     7561 taatgataac gagctgtgtc tacaagaacg ccatctaaat cgaatagtgc tgctttgatt
     7621 ttcatcgatt acctccttga aaatgaacga cttgaagtaa agatgcgatg tttttagctg
     7681 tgcgatgaag attatcagtg gcttgctgta aagcttcttg taaaggcaca gctgcaggaa
     7741 ggatcggaaa aacggccgaa atgccaacat ctgctgcttt ttcgcttcct ttaccaacac
     7801 ttccgcaaat agctatcaca ggaacgccct ctggtactct tctagcaatc ccaactggcg
     7861 cttttccttt tagactttgt ccatctagac gtccttcgcc tacgattacg aggtctgctt
     7921 gctgtacccg tttatcgaac tccagtaaat ccaatacgta gtcgatgcca gagactattt
     7981 tccctgaagc aaatgctaac agtcctgcac ccattccacc accagcacta ctgcccgatg
     8041 cctcgattac ttgaggggca aatctcatat aaaaatcatg catggcttgg tcagccactg
     8101 ctaattcatt ttctttcaag cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc
     8161 cgcataaaag attagtaaca tcagtaatga cagtgatttt ggctttaatc agctcaatag
     8221 gcacgttcgt tttatcaact gtagcaattt tacctagatt ttctcccagt gcttctactt
     8281 tttgattatc ttgatcgtaa aaagcataac ctagaccttc tgccatgccg atgccgccat
     8341 cgttggtact acttccgcca acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt
     8401 gtcgtatcag ttctcctact cctcgcgtgg ataacctaag tggctgttgt tggtttagtg
     8461 ggacatcttt caatccgacg atttctgcca tttcaaaaat cgcagtttga ccgtctgaag
     8521 cgtactggat ttctttgttt ttgccaaatg cattagtaac gactgctgtt tgccgcttca
     8581 accttaaact gtctgctaat gatgaaaccg ttccttctcc tccatcgcct ataggtagca
     8641 gatcatattc agcctgaaca aaaacttctt gaaaacctga ttgaatggct tgtgctgcct
     8701 ctaaggctgt cagactttct ttgaaagaat ctggcgcaat cagaatacgc attattttcc
     8761 cctccgttcc aagatcagtt ctttttccag ctggtattgg tctccattga caaaaatagg
     8821 taatgtttct ttcgtctctt tttttattac tatttgtttt tttgtcaaaa caaatagcaa
     8881 cctgtgattt ttccaaataa aaggaaaaga aattttttcc catgctgaag gaagtcttgg
     8941 agaaatagaa agagttccat tcgttactgt aatacctgaa aaaccaaata taactgctag
     9001 ccatagtgct cctaatgaag cagcatgaat tccgtcatct gatgaatgcg gatctggtcc
     9061 taaatcaatc aggcacgctt cttggaataa atgatacgcc cattccattt cttggatacg
     9121 gcaagcctct atggcgtgaa tcgctttact caacgatgag tcgtggattg ttttgttttc
     9181 atagtaatgc aaattcttag aaacgattgg ttgagaaaaa tactcaggta ataagtataa
     9241 cagcatgaca agatctgctt gcttgctgac ttggagttca tttacttcgc tacgggcata
     9301 atcaagtaaa atagactggc tgccaggacg ttctttgtaa gtggtcaaat caataactgg
     9361 cttactgaga tacgtgtcat cttgcggaat cagctgttct ttgttaggca ctggcaaata
     9421 taacttatct aaaaaatttt gacattcttt caaaaaaagc ggatcgccag cttgatattt
     9481 aacagctaag gccacattat aatgcgccag atagttcgta tatgcattat tgtcgatata
     9541 ttctgtgtat tcatctggac caatgactct gtgtatctct aaatgatcat cgacttttac
     9601 agctctgctc agccaaaaag ctgctgtttc atgaatgagc gtttttccct ttgtttctaa
     9661 aaacgattga tcctctgttg cttggacata ctcttgaacg gcataagcaa ggtctgcact
     9721 gatatgatgt tctgcggcag ctgaagcaac ttcttgtctt gttccggttt tgatattcaa
     9781 tgcagcaaat ttgggtgtct cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc
     9841 accttgatag tgataatctc ttgctttttg ccgtgcgcct tctaatcgat cataacggta
     9901 cagcagcaaa ttctttgcta cttcaggaaa ctgatataaa aagaaaggca ggataaaaat
     9961 ttccgtatcc cagaaaacat gccctttata accttcgcca gttagtccct tcgcccctac
    10021 actaacactt ggatcattgc ggggagtcat aatctgtaaa tggtaagcag cgaagtccat
    10081 cgctaactga tcgaaagaag aatcgccttg gatcgttatc cgatgctgat gccaaaaatc
    10141 tgtccaataa gacatcgact gctctaaaat ttctgtataa ctaacgcttt tctgggcttg
    10201 ggcaatactt ttcttaaacg ggtctgtttg ctgaagagag gtagagagca cgatgatctt
    10261 ctccagcgta aaggacgtat tttttctgat cttttctgaa aaggaagcgg tgatccggcg
    10321 attttttgct tggaatacac ctgctttatt gaatttagct gcaatcccaa tatcaatttt
    10381 actttctgtt gtctggtagg atgcactgat caaatcgtca ccaaatactc gcagttcttt
    10441 ttcaaccaac tgctgtgtac caaaattggt ttgctgtcca tcgatacccg ttttcacatc
    10501 tacttggcag tcttcggtca acggcttgat gaccactttc agggctgcct gatggagatt
    10561 attttgatca gcaacacgac aagtaatgac ttccactttt tttccttgag cagtttcaat
    10621 gagcgcatag cgtttaagct ctcccatttc taaatctagt gcccgctcgt atgcaagaac
    10681 tttcatcgtc tcaagagaaa aatgttcacc gtcgattttt atttcccatc tgatgacatc
    10741 tgggagattc actaactcgg ctgattctgc tcctaaaggt cggttatata caccagctgc
    10801 aaacattccc cttacttgat tccgatagtc ttcctcctgg cttcctctga taccaaaaaa
    10861 tccatttcca atggtcatct gactcgcata cttattgata tactctaaat caaactgttt
    10921 ttcttctact ttcataaggt aataacttct cctgtttctg ccgaacgata taaattttcc
    10981 accagattct ggatgacata tccttgccag ccatctgcaa tcatctgagt ctggtcaccg
    11041 agcgcccgat tcacaaaatg agccatactt agttcatgtg ggtcattaac tggcgtggtc
    11101 ttttgtaagg aaaccagctt tccatctaca tcttcgaata gttcgcctgg atacagatta
    11161 gcacctgcct gatcgccaca aaaagccaca ttcagtttgt cttctctgat attcaaagca
    11221 aacgaagtat tcagttggat gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca
    11281 tcttctactg tgaatttgct tggatcccat ttcccgaatt tccctgaatt ttttttcgta
    11341 ccaatttttt ggaagctgta cgcccgaacg ctttttactt taggaaatcc agtaatatac
    11401 atcgctgtat caagcatgtg aatgccataa tcgatcaaag gcccgcctcc ttgaagttct
    11461 ttgtcgataa acgttcccca gcctggaaca ccagaccgac gaagcgcatt cgcttctacg
    11521 taataaatat ccccgagaac atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa
    11581 cgatgctgga atccataagc caaaatctgc ccgtttttat cggctgtctc tgccatcttt
    11641 cttgcttgat cagctgtcat agctggtggt ttctcgcaca tcactgaagc attagcttcg
    11701 agggcagcca atacatgttc gaagtgaaag cgattgggtg tacagacact cacgatatcg
    11761 ggagaaatct cattcagcat ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt
    11821 cggcaaaagt cttctgctcg ctggctgact ggatcaacca cagcggccaa ttctaagaca
    11881 tctgatcttt tttgataata agcagcatgt actttttctg ctacttgtcc agcgcctatg
    11941 attgctactt tcttcattta tttctctctc ctttgcttat ttacttattg ttattctttc
    12001 tctattcttt ctctaaatca tttaaatgac gcgctgtttc cagacagttt ttcaagtatt
    12061 gcaatgattc ctgataaatt ttcacttcat tttctcctcg caaacgacat tcatatacca
    12121 tataaccttc atactcattt tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc
    12181 cagatcctgg ttggtagcga tgattatctg ctaaatggat atgacccaca tactcttttt
    12241 gcgtagtcaa tgcttttgaa atatcatctt cttcaatatt catatgataa aaatcagcga
    12301 tgatctgcac atgtttcaat tgattagagt caatgtaatg acgcgcatcc gctaacaaat
    12361 tgatcatatg gtcttggtat cggttcaaag gctccaaata aataaccgta cctgtttctt
    12421 cggctacttt atctaaataa cgaagtgatt cgctgactgc tttatagtct ccttctttgc
    12481 ttcggggtga aaccatcggc ggtaatcgat aagtgaacat tccccaagct gctgggataa
    12541 cgattccttt gcctccaacc tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac
    12601 cattaagacg ccgttcttcg ataaagtcac cgatccaccc atcgtagccg ttacaagctg
    12661 tcactactgg gatccctgtt tctttgattg cctgtttgac tgtgtcgata ttttctatta
    12721 acaacttacc gtcgatttcg tattcttcaa atccacagtc tttgataaat ctaaattttt
    12781 ctaaaatgtc tcttgggaaa aatgcttgat cttgcgtacc gaatttcatt gttctcctcc
    12841 tcggacttga acgcccattt tgatactttc ttctgggtgc tggtctacat aacgcatgta
    12901 agcttctgcg ctttctgcaa atggcacaac tggatcaatg atttcttcac aatccaagta
    12961 accgttcatc aataattccc aacatgtgtc ctcgattcgt ttacggttcc atcttgggta
    13021 atctgggttt ggttcactag atgctcgagc aaagacgatt tttgcattat tgaaatgtgc
    13081 ttcgcgacca agattaaacc cagctggaaa aggtttagca aaggcaacat aagaaatgat
    13141 cccgccatag ccgatcccta ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc
    13201 aataactgaa tctactccac gtttatctgt caattccttt agacgaaaac cgacatctgc
    13261 tttcatagga tctagcgtgt aatcggcacc gtgtttttct gctaattctc tacgctgcgc
    13321 tatgggatcg atcccaacga tggtagtagc acctgctttt ttagctaatt gaatcgcaat
    13381 ttgcccgata gcgcctaaac cgaagattgc tacagcatca cctgctcgaa catgtgcttc
    13441 ccggatacca cctaaagcga attgagcagg atcgtaacat actgcatttt tccattgatc
    13501 caaccgatca agtttacgta gtttataatt atcaacaccg tttgtaatca gcgtttcacg
    13561 aatgggtcca taagtgcaga ctaactctcc tttttgatac tctgtcacca aactgcccgt
    13621 ttcttcgatc gtaccgacaa tcatatttcc taattgaaat tcgccaaata ccacaccgcg
    13681 ttggcttcct tcttctcttg gcaagaatag gcgccattct tctgaaaatt cttcatcgat
    13741 aaatggactt aatcctctaa agtcgacaat ttctgttcct ggttttggag tagcgaaaat
    13801 tactcggata cgtacttcgt tcttttcgat ctcacggtct tcgtaatctt tgaatccggc
    13861 tattcttggt tctaatgcaa ctaatttttt catcttttct ttcctctttc ttttcttttt
    13921 atccttttac gccgccttcg actaaatcac ctttaataaa tcgttcagat aacgcataca
    13981 taatgatgac tggtaaagcg gtcaccaatg aagcggccat cattcgtccc caaatgtagt
    14041 ctggtgtact gaataatgtg ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca
    14101 ggaaaatcga agcaaataaa taatcattcc acgaaaccat aaagcaataa acgaagacag
    14161 aaatgattcc agaaatagcc aatggtataa tgatccgaat aatgatctgg atccggctaa
    14221 gaccatcgat cattgctgct tcttcaatat cagtcggaat cgtgtcaaaa taacttttta
    14281 gcatgaatat cgcagtagga agtgtttgaa cgaccatcgt gataatcagt gctgtattgg
    14341 tatcatacaa acctagaccg gaaatgattt taaataatgg aacaacgatc aaaataccgg
    14401 aaaacatata aacggtgtaa aagctagcat tgattttcga gcgagctttg aagcgtagtt
    14461 ttgacaaagc gtatgccccg ctgatcccaa tcactactgc agttacggag gctatcaagg
    14521 agatcactaa actatttttg aaatacatga tgaatggaaa aatctcagca ttgaaaatat
    14581 cctgataatg ttcaaaagtc cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt
    14641 cttgtgaatc cttgaatgat gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta
    14701 atagcaacaa aatcgtcgcg taaaataaga ttttcctgct ctttttattt ttcatttcaa
    14761 gtccaccttc tttcgtgaga gcaagacgac cgctgcaata atgatgaaca atagaatcga
    14821 tatagctgct gcttttccaa gatcattgaa cgcaaaggct gtctgataca aataaacgcc
    14881 taagatattg actttattcg tcagtagata gacatctgta aacatataga acatccagat
    14941 agatcgtaaa acaatcacag ttgctagtac tggtttgatt gccggcagtg tcacgatctt
    15001 gaacttctgc caagcatttg caccgtccat ttcagctgct tcataaagcg ttttgtcgac
    15061 tgtttgcaaa atagccaaaa atgaaataaa cgcatacgga tagtatctcc agatagcaaa
    15121 caaaacgacc ataaagaagc tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa
    15181 aagctgcaac ctatctacta acagatcatt gacgattcca taactattat tgaacatata
    15241 tttccaagca aaaatcaaag aaatagatgg cgtaacataa cttaataaaa tcagtgaacg
    15301 caccgttttc ctaaatctga acttgcggtt aagcaaaata gctacggcta atcctaaagc
    15361 tgtactgcct gctacaacta agaatgtgta agcaactgtt aaacctaaag aacgataaaa
    15421 ttcactatca cttaaaattt caagataatt gcttaatccg ataaaaacgg cgtctgagtt
    15481 aggattcagc ggcatatcga aaaaactgat ctgaatattg gaaagcattg gaatcagcac
    15541 taaaacagct aataaaagca tgcttggtgc taaaagtccc atggctaatt ttaagtcaga
    15601 atgtttcctt ggacgattca ttgaaacctc cccctgataa tctgccaata gctatctact
    15661 tttttcgaac accattggca gaacttcttg ttatttcgta tttttttgtg cgtcagctaa
    15721 agcttgatcg acatcttcgt ttccgacagt tacttggttc actgcttttc cgatcacacc
    15781 tgagcttgtg atatctccca ttttcgtgaa attcttttcc ccgactagtc caaagacttg
    15841 tacttcatca aaagcactag caatctcaga tggcagctcg ccaaaagact tgatgacttc
    15901 attttctttg taaccatcca gttcgactac ttgtttattc actggttgag caccaccagg
    15961 agacatcaag acccactttt ccatgttttt cggttgagat aagaaagctg tgaatgcttc
    16021 agcagcctct ttttgtttct cgtctagacc agaagaaatc gtcaatccag atactgtgcc
    16081 atagacagct tgatcttttt gtgttgggat agcaaaaccg atattttctg agttccccgc
    16141 ttcataaaca gaaggcaaaa tgtaagttga atagattgcc attggtgctg ttccattcat
    16201 gaaagcatct ttgatttctg tcacatcatt agagcctggc atcgtgtatt gggataagtt
    16261 ttgataaaac gacaatgctt ctctcatctt ttccgtatcg atcgttacct cgccttcatc
    16321 atccaaaaca tttgctccat ttgacaaagc aaattgagag aacgcttgct cactcattgt
    16381 tccttcagca gtaggcatag caattccata ttgtttattc tctttatctg tgaaatgttg
    16441 agcaattttt aaaatatcat cccaattttc tggttctgaa aaaccagctt ctgataattt
    16501 ttctttgtca taccaaatac cttgaaccca gccgctaatt ggtgcagcaa tatagctttt
    16561 tccatcttct gaacgaacaa ggtttttggc tccgtcataa taattatctt ctccagcttc
    16621 ctcgataacg ttttggactg ccgtctgatc aatcagttca tctttatcca tcactttagc
    16681 aaaatcctga ctgacttcca ttacttctgg taatttaccg gaacgagcta acgtaacgat
    16741 ttttgtattg aacgctgatt cttctaccgg cacttgcttg attttgatgt ctggattttc
    16801 tttttcaaaa tcagcaacca gttcattgat cacacttaat cgttcctgtt cgacagaaga
    16861 gtgcatgaat tgaatcgttg tctgttgtga ttcgttagag ctttgacttt caccacaagc
    16921 agtaagaaga aatccagtag ctagcagaca ggtagtaaca ataatttttt tcattttttt
    16981 ccacctctta ttttattatt ttcagccaca tatattcata cggctggaat atcaactttc
    17041 gttcttgact gcaatggata atttgtccag aaaacaggtc taggtaatct ccttttggta
    17101 aaagataacc tgctgattga ttggaaaaat tatggataac taagactttc tcttccttcg
    17161 cttcgcgaat aaaactgata aaagaatctg ctgagtccag taagtaaatc aaactattcg
    17221 gatggaaggc tttttctttt tttcgaatag atagcatctg ggtcaattca ccatatactg
    17281 ctgttcttaa tggatctgtc tgcaactctt tctcaatatc tgctaacaaa aatttttgcc
    17341 gattgatttt tcgattctct cctgtttttt ctacgccttt gtaatcattt cgacttccca
    17401 gcacactttg aatataaatt gccggcacgc ctggtaacgt taccaaaaat gaatgagcag
    17461 ctaaaaagcg tttgcttctc cattccgtcg tatctgttgg agaacttaac gcatctagat
    17521 acgtagcgtt tatctcatat ggactggtcg ttccatctgg gtttttcttg tagtttactc
    17581 gggcaccctc tttcttcagc tgttccacta attcaaggat cgttttttcc ggcagaatac
    17641 ctcgtaccgg attcaatccg ataccgtcat gggaggctaa aaaattaaaa aaagttgttt
    17701 ttttcgatgc gaaatcgatg gtttttaacc attctttcaa cttacttgta ttacccgaac
    17761 gaatcgcttg aagggtcaaa ggtggtagcg aaaactgata gaccatctgt gcttcattct
    17821 cgccattccc aaaataactg atattatctt tgtgtggcac atttgtctca gtgatcaata
    17881 ccgttcctgg agctgcttca tcagcgattg cacggaataa ttggatgatt ttatgcgttt
    17941 ctggcaggtg tatacaagaa gttcctagtt ctttccataa aaatcccaca gcgtccaagc
    18001 gaataaatga agcgcctttt tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa
    18061 cttctggatt tttaaaattc aagtctactt gatcttcgct aaaagtcgtc catacgtgtt
    18121 tttctttccc gtttttgtca atgaacgttg taagtaacgg gctggtcctt ggtctagtca
    18181 cgctgcttgt atctgtatcc agtggcaaat caataaaaaa atctttgtac gtatcattca
    18241 agtttaaata ttcctgaaac caagtacttt cggcagacat atgattgcag acaaagtcga
    18301 acattaaccg tgtttcgctt ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt
    18361 tgattgcctg ataatttaca acggagaaac catcatcaga tgtatatggg aaaaatggca
    18421 agaaatgtac aactctaaat gtatctgcca aatacttttg ataaaatttc ttaaatgtac
    18481 gcaaagttgg ttgctgctct tcaaaaaatt gatccccata agtaatcaag aaaatatctt
    18541 cttcgtccca atgcagtttg cctgcctggc ctttcttctg ctgataaaca ctgacttttt
    18601 gttcaatcat agaggcggtt ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa
    18661 gttttgtata catcggattc ccctttcttt tttgggagcg ttcccaacga tttgatcata
    18721 ttatggaaaa cgcttactgt caatagcatt tttttaaata attttgatat acacaaaaat
    18781 acaaataaga aagcttatgt atcaataatt aatagcttct taaaaaaata tggtctctca
    18841 tgatcatgca agctattcct aaattttcaa aatcatagac aaaaaagaat gtctattgta
    18901 ctatggaatt attctaaaac gctcccaaaa aggaggtgca tataaaattg aaacctacta
    18961 tttatgacat tgcgaaaaaa gctaatgtct caaaatcaac tgtctccaga gtattaaata
    19021 accagccaaa tatctctgat aaagcaaaag caagtgtact tcaagcaatc aaagaattga
    19081 attatgaacc gagcaaaatt gcaagaggac tgtcaggaaa tggttttgac gcgattctag
    19141 cattatctca tcgtacttct catacgacct atggcaatcc ttttttttca gatgttattc
    19201 aatccatttc tgccgtttct gaacaaaata acttcgactt gattctacaa acggcaaaaa
    19261 caccagaaga agagctagaa aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa
    19321 ttattatttt aagctctacg atggatgaaa cacttctttt tgaattggat caatacaata
    19381 ttcctattgt ggtaatcgga aaagtcggaa acgactacac gaacatctac tcagtggaca
    19441 cggataattt ccaggatagt tacgatttgg tggaaacact gatcaagaat ggacaccgtc
    19501 atatcggctg tctccatgct ccgacagaag tttatgtatc caatgatcgt ctcaatggtt
    19561 accgtcaatg cttatttgat caccacctag atatccgtaa tgaatggatt atagacggcg
    19621 gctttcgctt agaagatagc atggcagcaa tgaatgattt aatgaagcta cgagaaagac
    19681 caactgcgat tttttctacc gatgcattaa aatcattgag tatttacaaa gtggcagaac
    19741 aatctggaaa gcagatacct gatgatcttt cgatcgcagg attcagtgat cccttattgt
    19801 cctccctatt ttcgccacag ttgactcgga tagacattcc gacaaatcag ttaggaatca
    19861 aagccacaga acttttattc caactaatcc accaccaata ccctgaagaa aaaagaatca
    19921 ttctttcaac cgagattact ctcacagatt cgatccaaca acaagaaatg tagaacagaa
    19981 ttataaaaga acagaaacac tgaggataag tcagttactc tgttcttttt tcaatcactc
    20041 atccctcgtt accccctccc ggtattttag cgaatttttc ttattttcca tctgttttag
    20101 atagctgtgg ataactataa tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac
    20161 tatttttaac cacgttgtgt ataagttttt atagttcgtt tttttaaaca catttaccct
    20221 tatttcaatc catgttattt ggggatactt atctctaaaa cataaataga ataacagaca
    20281 tgtctactgg ttttgtttta ttcgaggaat tattcaataa tagctagcaa aatattaaaa
    20341 ataaccgctc tatcttccca tcaatttatt ttgcattcaa aaaatgatct atctttactt
    20401 ttgttgattg acttctcttg gaagatgatc gttccgatac cttaattgga tacgaaccga
    20461 tgttgttaag atcctttccc acttttcttc tagttagaca aattatttta gacaaaccaa
    20521 aaaagaggtt gtgacaataa tttgtcacaa cctctttttt aaaatatcta ttttgtttga
    20581 ttgttcattg cgtcttctgc agccatttgc gcttcaatat ctgaaatact gtagacattt
    20641 tcgcttgcta caccgacttc ttttggttcc atgtcacgat aacgttgcat accagtacca
    20701 gctgggatga tcttacctat aataacattt tctttcaaac caagaagagg atctttctta
    20761 ccgcggatcg cagcatccgt caagacacga gttgtttctt ggaatgatgc agcagataag
    20821 aaactatttg tttctaatga tgcttttgtg atacctagta agacaggtcg agaagttgca
    20881 ggaacgcctc ctgagactag cgtattgtag ttttgatctt taaagtctgc gatgtctaat
    20941 aatgtacctg gtaagatatc tgtatcacct ggatccatta cacgaacttt acgcagcatt
    21001 tgacgaacca ttacttcgat atgcttgtcg ccgatttcta ccccttgcat acggtaaaca
    21061 cgttgtactt cacgaagcaa gtagttttca acagataata catcgcgaac ttgcaacaat
    21121 tgttttggat cgattgatcc ttctgtcaat ggtgcaccac ggtggatcat atcgccttct
    21181 gctactttca tacgtgctgt ataaggaaca gtatacgtac gtgtatctgt ttttcctttg
    21241 attgttactt ctttggtacg tgttgcagga tcttctgaga tatcgatgac atcccctgtt
    21301 acttctgtga tcactgcttg ccctttcgga ttacgtgctt caaagatttc ttggacacga
    21361 ggaagacctt gagtgatatc gtctccggca accccacctg tatggaatgt acgcatcgtc
    21421 aactgagtac caggttcacc gattgattga gcggcgatcg taccaactgc ttctccgact
    21481 tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat gtttgcagac accatgtttt
    21541 gtattacatg tgaagacaga acggattgtc actgtttcga cacctgcatc aacgatttgt
    21601 tttgcgatgt cttcggaaat caattggttt cctgcaatga tcaattcatt tgtttcagga
    21661 tgacgtactt ctttacgggt gtaacgtccc actaaacgat cttctaatga ttcgatgatt
    21721 tcatttccct cgcggatggc ttggatttca agacctcggt cagtaccgca atcttcttca
    21781 cggatgatta catcttgggc cacgtcaacc agacgacgag tcaagtaacc tgaatcggca
    21841 gtcttcaagg ctgtatcggt catcccttta cgagccccgt gagttgagat aaacatttcc
    21901 aagacggata gtccttcacg gaagttcgaa atgattggca attccatgat tcgaccatta
    21961 ggagcggcca tcaaaccacg cattccggca agctgtgtaa agttggaaat gttaccacga
    22021 gctccagaat cagacatcat gaagattgga tttttcgctt ccaaaccttc catcagtttt
    22081 tgttggatgc tgtcttttgc agcattccaa acagcaatga cacgttcata acgttcgtca
    22141 tctgtgatca gaccacgacg gaattgtttt gtaatagttt caacttgttt atgggcattt
    22201 tcaatgattt cttgtttttc attcagtaca ctgatgtcag cgatccctac tgtgataccg
    22261 gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt caagcatctt agaagtttct
    22321 gttactttga atcgtttgaa gacttctgcg atgatgtttc ctaagttttt cttcttgaac
    22381 ggtaatacta attcttgctc tttgatatgt gcaggaatat cagtaccagc ttcaacaaag
    22441 tatttgtccg gtgtttggac ggtcaagttg aagtctgttg gttcattcag ataagggaat
    22501 tcaggaggca tgatctcgtt gaagatgatc ttaccaactg ttgtgatcat cgtacgttct
    22561 ttttgccatt cagtaaatgg tttttcacct aatgagttag ggttcacacc gatacgtgaa
    22621 tgtaaatgaa catatccgtt gcgccatgcg ataacggctt cgttttgatc tctgaagacc
    22681 attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt agtagttacc tagaaccata
    22741 tcttgagaag gagtaacaac tggtttacca tctttagggt tcaagatgtt ttgagcagct
    22801 aacatcaaca tacgtgcttc tgcttgtgct tcttcattca atggaacgtg gacagccatt
    22861 tggtctccat cgaaatcggc attataagct tcacatacaa gtggatgcaa acggattgcg
    22921 cgtccttgta ctaaaactgg ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc
    22981 cggttcagca atactgggtg ttccttgatt acgtcttcta aaacatccca tacttcgtct
    23041 tcttgacgtt cgattttacg tttcgcattt ttgatgtttg aagctagttc gcgttgaaca
    23101 agctcacgca tcacgaatgg tttgaataat tcaatggcca tttcttttgg aagtccacat
    23161 tggtacatct tcaagaaagg tccaacaacg ataacggaac gacctgagta gtcgacacgt
    23221 ttacctagca agttttgacg gaaacgacct tgtttccctt tcaacatatg agaaagagat
    23281 ttcaatgggc ggttacctgg tccagtaact ggacgtccgc gacgaccgtt atcgatcaat
    23341 gcatctactg cttcttgcag catacgtttt tcattttgaa cgatgattcc aggtgcattc
    23401 aagtctaaca aacgtttcaa acggttatta cggttgatta cacgacggta aagatcattc
    23461 aagtcacttg ttgcaaaacg tccaccttct aattgaacca ttgggcgtaa atctggtgga
    23521 ataacaggga taacgtccat aaccatccag cttggttgat ttcctgaagc gcggaatgct
    23581 tctaaaatat ccaaacggcg gattgcacgt gtacgttttt gtccttgtgc agatttcaat
    23641 tcttctttca attcggctgc ttctgcatct agatcaacat tatcaagtaa ttgtttaatc
    23701 gcttctgcac ccatagcagc ttggaattct tgtccatatt gttcacgttt ttcgcgatat
    23761 tcgcgttctg tcaacaattg ttttttctct aaattcgtat ttcctggttc gatgactaca
    23821 tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc
    23881 atacgtgaag gaatcccttt aaagtaccag atatgtgaaa caggagctgc taattcaata
    23941 tgtcccatgc gttcacggcg aactttcgaa cgagtaactt ccactccgca tcgatcacaa
    24001 acgattcctt tataacggat acgtttgtat tttccgcagg cacattccca gtcttttgta
    24061 ggaccaaaaa tccgctcaca gaacaaccct tcgcgttcag gttttaaggt acgatagtta
    24121 atcgtctcag gtttttttac ttcaccgtaa gaccagcttc tgattttttc gggagaagcc
    24181 aaacctattt gcatactttc gaatttattt acatcgatca aaaggggttc cctccatttc
    24241 atttaatggc aaaccgcaag acggctgatc ttcgaccgcc ttgcagctca tagaccggat
    24301 tagtctaaat tatcttctgc ggtttcaaag ttttggatga catcgtcttt ttcatcttcc
    24361 acttgttcag cagctttctt ttctaattct ttggcagttt gctgttcggc aaattttgtc
    24421 aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct
    24481 gtttcttcga tatccaagac acgcatgtcc agacctagtg attgcaattc tttcaccaat
    24541 acgcggaatg attccggtac acctggtttt ggaattggtt cacccttgac gattgcttcg
    24601 tatgttttca cacgtccgac tacgtcatct gatttgtatg tcaagatttc ttgtaaagta
    24661 taagccgcac cgtaagcttc cagtgcccat acttccattt caccaaaacg ttgtccacca
    24721 aattgagctt tacctcccaa tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt
    24781 gcatgcaatt tgtcatcgac catgtgagca agtttgatca tgtacatcac accgacagac
    24841 acacgtccat caaatggttc acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg
    24901 gccataccgg cttcacgaac tgtttcccat acgtcttcat cacttgcacc atcaaatact
    24961 ggcgttgcta cgtggatacc taactgacgt gcagccatac ccaagtgtaa ttccaatact
    25021 tgcccgatgt tcatacgtga aggcacccct aaaggattca gcatgatatc gattggtgtt
    25081 ccatcaggta ggaatggcat atcttcttcc ggcataatac gggatacaac ccctttattt
    25141 ccgtgacgac cggccatttt atccccttca tggattttac gtttttgaac gatataaaca
    25201 cgaactaaca tgtttacgcc tggtgacaat tcatctccag cttcacgagt aaagattttc
    25261 acatcgtgaa cgataccgcc gccgccatgt ggtacacgta atgatgtatc gcggacttca
    25321 cgagcttttt cgccaaagat cgcatgcaat agacgttctt ctgcagataa ttctgttacc
    25381 ccttttggcg tgactttccc tacaagcaaa tcgccatctt ggacttctgc accaatacgg
    25441 atgataccca tttcgtctaa atcttttaat gcgtcttccc cgacattagg gatttcgcgt
    25501 gtgatttctt caggtcctaa ttttgtatcg cgggcttctg attcatattc ttcaatatgg
    25561 atagaagtgt atacgtcatc ttttaccaaa cgacggctca tgatgatcgc atcttcatag
    25621 ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt ggcctagagc catttcgcct
    25681 tgttccatag atggtccgtc tgccagagta tccccttttt cgactttctc acccaaatga
    25741 acgattggac gttggttgta gcttgttccg gagtttgaac gacggaattt tgttacatcg
    25801 tatttatcta atgcaccgtt gtcgcggcgt acgcggattt cagaagcatc aacgtattca
    25861 acgacaccgt catgtttaca taacaaagca gctcctgagt catgcgctga tttgtattcc
    25921 ataccagtac caacccatgg tgcttgagga ttgatcaacg gcactgcttg acgctgcatg
    25981 ttggcaccca tcaaggcacg gttggagtca tcgttttcca agaaaggaat acatgctgtc
    26041 gcaacggcaa ctacttgttt aggtgatacg tccatgtagt ctactttatc aatagatact
    26101 tctaagtttt cactttgagc acgagccata acaacgtctt gagcaaatgt tccatcttca
    26161 ttcaatgggc tgtttgcttg ggctacgatg taatggtctt cgatatcagc tgtcaagtag
    26221 tcgatttggt cagtaacacg acctgtttca cggtctacac gacgataagg tgtttcgata
    26281 aatccgaatt tatttacttt cgcataactg gacaaactat tgatcaaccc gatgtttggg
    26341 ccttcaggag tttcgatcgg acacatacgg ccatagtggg agtagtgaac gtcacgaact
    26401 tcataaccgg cgcggtcacg agtcaatccg ccaggtccta aggctgaaag acgacgtttg
    26461 tgtgtcaatt cgccaagagg gtttgtttga tccatgaact gtgacaactg agaagaacca
    26521 aagaattctt tgatacttgc cacaactgga cggatattga tcaattgttg aggtgtcaat
    26581 gtttcagtat cttggatcga cattctttca cgaaccacac gttccatacg tgctagcccg
    26641 atacggaatt ggttttgtaa aagttcaccg actgaacgga tacgacgatt tcctaagtgg
    26701 tcgatatcat cgacattgcc gataccttcc atcaagttca agaaatagct cattgaagca
    26761 atgatgtcgg ctggacgaac cgtttttaca gctgcttctg gatagccgtt tccgatcacg
    26821 tttacttcac gttcagggtc tcttggtgaa aatactttga tgacttggac agtcattgga
    26881 tctgtgacca caccgtcttc tgatggatag taagtcactg aatttaggcc attatcaatg
    26941 aaaggagcta gtgtttccat gacttggtga gtcagaactg ttcctttttc aacgatgatc
    27001 tcgcctgttt ctggatcaac taatgtttca gctaatgtca gattcaatag acgtgttttc
    27061 aaatctaatt ttttgtttac tttgtaacga ccaacgtttg ctaagtcgta acgttttgga
    27121 tcgaagaaac gagcattcag caagttacgt gagctgtctg ctgtttttgg ttcgcctgga
    27181 cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc ttgagtcgct tgcgttttta
    27241 tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc
    27301 gtatcatcgg aaccaaatcc taacgcacga actaatacgg ttaaaggaat cttacgtgta
    27361 cggtcgatac gtacataaga gatgtctttt gcatcagttt ccatttctag ccaagctcca
    27421 cggttaggga taacagtaga accgaatcct tcttttccgt ttttgtcgac ttttccatgg
    27481 aaatagacac ctggtgaacg gactaactga gaaacgatta ctcgctctgc cccgttgatg
    27541 atgaatgttc cttgttctgt cattaatggg aaatcaccga agaatacttc ttgtgctttg
    27601 atttcgccag tctcgcggtt tgtcaaacgc aatgttacat gtaatggtgc agagtagttt
    27661 gcatcatgcg cacgagcttc tgctactgta tactttggtt ctttcaattc ataatctaca
    27721 aattccaaag atagattgcc attgaagtca tcgattggca aaatgtcttc aaacatttct
    27781 ctaagtccct catctaagaa ccattggtaa gagtctgttt gaatttcgat caaatttggt
    27841 aattccaaca cttcactgat tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt
    27901 actacgtgtc cagccaagct cttcacccct ctaaaatgtc attcaaaaag actacccgat
    27961 caattgttac aataatattt caaatattat ttttaggtaa caacagacat tttttgcggc
    28021 ttttaggcaa aaaaaaacca aaaatagaac attctcttga aaccttactt cgtgagcttc
    28081 tacttttgtt tccttatatt aaagccgaaa cggacaatat ttcgtttctg ccttttcttc
    28141 atcagatagt ttttgcaact tcatatacta cacttatttt ctataaaagt caacacttcc
    28201 tggcgtttct tctgcttttt cgcaacattt tcttaatgtt ttcaatttta tgttttcaat
    28261 tatctgacaa ttcttaattg acagaccttt ttatctcggt tatactttag aaaattcatt
    28321 ttgtggagga acttctatgc gaaccaacat caaccaaatc gcaccaacac aactgaataa
    28381 cttttacttt agctacttta gataggtttg tattcatcat gatggataca aacgttaaac
    28441 gacagtttgt atctatgatg gctaaagaac tttgtattgc agcaaaaagc cacgtagaca
    28501 aaatgctaag tggctgagca gtgaaaatac aaagtaaaag tatcaagcat tctacttttt
    28561 caatttattt tccgactttt caatccattt ggacattttg tctgaatgga ttttttgttt
    28621 cccaaaatta aggaggaaaa agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc
    28681 ctgctactag ctttaggagg ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga
    28741 caaaaaacaa ttggcgtatt gcaacttgta caacataact cgttagatgc tgcctataaa
    28801 ggattcaaag aaggattagc agaagaaggg tataaagaag gcgataatct aacaatcgat
    28861 tatcaaaatg cccaaaacaa tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa
    28921 gaagatcctg atttgctact tggaatcgcc actccagcag cacaatcttt attaaatgaa
    28981 acaaccacta tcccgatgac tgtcacagcg gtcacagact tggaacaagc ccatttagtc
    29041 acatccaatg aaaaacctgg cggcaatgtg actggcacga cagatatggt gccaatcgaa
    29101 aaacaaatcg gcttactgtt aaatatcgtg ccagacgcca aaacaatcgg catcatgtat
    29161 aacaacggtg aagcaaactc taagatccaa gctgatttgg cagaaaaaga attgaaaaaa
    29221 gcgggcgtca aagtcaaagt actgacagca aattccacaa atgatgtcca acaagtgacg
    29281 aaaagtctag ctaaagatgt agatggaatc tatattccta ctgataacac gttcgcaagt
    29341 gcggcaagtg ttgtcggtga agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca
    29401 gttgatcaag tcaaagacgg cggtctagca acagtaggaa tcgactacga aagcttgggc
    29461 aaacaaacag ggatcatggc agcaaaaatc ttagacggca agaatccaag tgaacttcct
    29521 gtcgaaacag cgaatgattt ggaactgtat gtcaataaag acatggccaa agcattaggc
    29581 atcaatccag acagcatcaa agaagctgaa taaaacacag gaggattctc atgacaaatt
    29641 tgctctctct tttcttatca tctgcatcgc aaggggtctt atgggcccta cttgcgattg
    29701 gtgtatacgt cactttccgt atcttggaca ttgccgactt gactgctgaa ggaagctttc
    29761 ctttaggtgc agggatcgcg gcagtaagta ttacaaatgg ctatcatcca ttgacagctt
    29821 gtcttctagg ctttttaggg ggtgcggcag cagggcttgt ttccggactg cttcacacaa
    29881 aattgaaaat tcccgccctt cttgcaggga tcatcaccat gactggttta tattctgtca
    29941 catcccgtgt gatgggtgcg ccgaacatct ctttattggg tcagccaacg atctttcatt
    30001 gggtcgagtc attcggcata acaaaagaca acgctgtact gatcgtcggt ctgctttttg
    30061 ctttgatcgt tgtatgttta cttgttttgt tcttacgtac agaaacagga cttgctattc
    30121 gtgcaactgg cgataatctg gcaatgagcg aagcaaacgg gatcaacaca gataatatga
    30181 agatcatcgg ttatatgatt tctaatggat gcattgctct ttctggctcc ttacttgcgc
    30241 aaaacaacgg ctttgctgat ttaaattctg gtattggaac gatcgtaatt ggacttgctt
    30301 ctattatcat cgcagaagtt ttgttacgta accagccact atttctccgt ctcttgactg
    30361 tcgttttagg tgcggtcatt tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg
    30421 aaccaagtga ttcgaaactt gcttctgctt tcgtgctagt gatctgtctt tctttcccgc
    30481 agatccagca aaaattccat ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga
    30541 cgtcttaaca ttggaaaatc tccatcatac ctttgaaaaa ggaacgatca acgaaaatca
    30601 tgtcctgaaa ggcattaatc tttccttaga tcccggcgac ttcgtgacga tcatcggtgg
    30661 aaatggggct ggtaaatcta ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca
    30721 gggtcaaatt ttattgaatg gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa
    30781 atccatcagt cgcgtattcc aagatcctaa actagggaca gccgttcgtt taacggtaga
    30841 agaaaatctt gctcttgcta tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa
    30901 accacaagac cgaacatttt tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa
    30961 tcgtttatcc tcagaaatcg gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt
    31021 gatggccaca ttacagcgtc cagaactgat cttattagat gaacacactg cagcgcttga
    31081 ccctgctact tccaaaacgg tgatggaatt gacagaaaag ctgatcactg agcagaatct
    31141 cactgctttt atggtcactc atgacatgga agacgcgatc cgctatggga gtcgactgat
    31201 catgctgcat caaggaagga tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac
    31261 agtgccccaa ttattagcgt tgttcaaaca aaacagcggc acggcattaa aagacgatca
    31321 agtactttta tcttcataat cagaaagagg atgggacaaa aaatgaacgc ccaccctctt
    31381 cttttttctc tttccttctt tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa
    31441 ctattttgtt ctctccttct tcctgataga atggatggga aaggagcttg atgccatgac
    31501 aacgaaagac aaggtacttc aattattgaa aaagacacca gattttcttt ccggagaaaa
    31561 gttggcacag caactggaag tttcccggac aagtatttgg aaagccatca aagaattgga
    31621 aaaggcaggc tatcgttttg agcacgggcc tacagggtat cgttacctgc cctctgacgt
    31681 actagatgca aaagaaatcc agcaagagct acggtcgttt attcctgatt tgaaagtaca
    31741 aacgatgttg acttctgaat ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga
    31801 ttctgtttcg aagttatttg ttgctgatac acaagaacaa gctcgtggac gttttggacg
    31861 gccttttttc gctgaacctg gacgaggaat ctacatgagt ctcttgctcc atccagacaa
    31921 acggtttgat gaattgcctc aatatacgat catcgcagcc agtgcctgtg tgaaagccat
    31981 tgaatctgta accggtcgtt ctgcttcgat caaatgggtc aatgatatct acatggatgg
    32041 aaaaaaagtc tgtgggattt tatctgaagc agtcagtgat atggaaagcg gcaggatcag
    32101 tcatatcatc attgggatcg ggcttaattt ttctgttcct caaaatcatt ttccagctga
    32161 tctccaagaa aaagccatct ctgtctttcc cgacggacaa gctagtgtca caaggaatca
    32221 gctaatcagc aaaatctggc aagaattgtt cacttatctt tctcacccag aaaaagcgtt
    32281 ggacttctat aaagaaaaat cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg
    32341 aacgacttat gtcggtcttg caaaagaaat cggtgatcat ggcgaactga tcgtccaaac
    32401 aaatgaacag aagatgagtt tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca
    32461 ataattatca gatttcacaa aaggacacct gatctcatac agtgtccttt tttctgaatt
    32521 ctcttcaaaa gactctcctt attctatcag ctgacagttt ctctatcctc tagaaaaagg
    32581 tattctaaaa atatctataa agcatggtac actttcttta aatgaatcaa tgcaaaaaac
    32641 aagagagtca tcagtttaca cgatcaaaag agatagcagg ccgcttcctt tttcaatctt
    32701 atagatagac catgggtaac gataaaaaca aaaaagaact tgcgacttat tttgccgtaa
    32761 gttctttttt agctagtgcc ttaatctctt aatcccaaca aacgtctcgc atagatcacc
    32821 tgaccagagt gcatagcagc atcatcaata atagaaacga gacgcattcc tcgtgtgact
    32881 ggtggtgtcc agttctcgtc aattacttca tctagctcgg aaggttccag ccttcttaag
    32941 taagaaatag caaaatcagc agctcctcgc aagtatccta gcagatcttc tatttgatta
    33001 gcttggatct gctttgcttg ttccagacta tgcttccaat cttgttcgtt atctgctatc
    33061 gtcatcggaa accggctttc ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa
    33121 tcagcaatct ggaaatctaa ttctcgagcg gtatgccaag ccagccaagt cattgatttg
    33181 atctgcgggg ctctatcttc cactggaaaa cgattggcct cctcaataga gacgccttca
    33241 aacaaacgtt caaaccgatc gtatgcccga tccacactct cgcatagcat atctaaataa
    33301 tccatttatt ttcctccttt ttatttatcc tacttacttt ttcctaattc taggatacct
    33361 acttatatgt tactacacac ccttattttt tacactttaa cagtcaccat catcaatagt
    33421 ttggagattg aaagaaaaag atagtgtcga actatcaatt gaacaagaag caacaatatg
    33481 aacgtttcat taatattctt taaataaaca atgatttttc aaacgaacag tttaagacta
    33541 ggagaaaaag agtataatag gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa
    33601 agggcaagtt tactagtcct agttgctttg ttgttcaata tcttttcgta tggtgtggcc
    33661 gtttcagcgg aaacatacat tcctaaagac cctgggaata tttataatga tctaactggt
    33721 aactatggct tgttaggaat tgctagtcaa ttccacgtat ttgctaaaaa taagacaact
    33781 gtgaatgcac atacagacgg aaatatagca acacatgaac ttgatgcaaa aaataatttt
    33841 ggtacagagc ttcatgaagg tttattgaac caagaaataa attatataca aaaaactgat
    33901 tctttcgcta attcttcagg catacctaca ggtagcacaa gaatgaataa atttgtcgtt
    33961 ggcagtaacg tggatgtagc acttcaagat ggtcatccta cagtcaatgg atccaggctt
    34021 gatcacttaa ctccagatga tttttataaa gatcgtgctg ggcatgaata tattgatttc
    34081 gagcaggaat ttgctaaatt gaatgtagca gcaaatgatc ttgcaacgat cactccagct
    34141 aaaacatata ctgctgcaga ttttccagac atgaacaatc ggacaatcga tttaactgat
    34201 ttatcagata ccgggttttt attggtaaat atcgatgcag aagtgttaac gatgaatacg
    34261 ccacttcaaa tcattaatcc aaatgatcaa gtagttgtat ttaatgtcat caacagttct
    34321 tctgcattga acgtacagtc accaatcaaa tacaatgatc gttcaaatca tgaaacagaa
    34381 gatttttcgg atgcgaatat ctcttggaat tttggaaatg agatgactga tttgacaatc
    34441 agtgctcctt ttcaaggaac aatccttgcg cctaatgcta ctatccgcat tactcaaaac
    34501 caagacggaa cgattatagg aacaaatgtg attttagatg cagctaccaa tcgttgggat
    34561 cctaacgaaa tatttatcag cgacaatggt acagatacga cagacacaag cgacagtaca
    34621 gatacatcta ccacaagtga tagctcagat tcatcaactt caagcgacag tacagatact
    34681 acctctacca caagtgatag cacggataca tcagcttcaa gcgacagtac agatactacc
    34741 tctaccacaa gtgatagcac ggacacatca gcttcaagtg acagtacaga tactacct
//
LOCUS       DAZKCN010000016        34585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000016 DAZKCN010000000
VERSION     DAZKCN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.016"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8K3_000762"
     CDS             212..604
                     /locus_tag="ACG8K3_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9144665.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8K3_000763"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8K3_000763"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP9144666.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8K3_000764"
     CDS             2698..2943
                     /locus_tag="ACG8K3_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144667.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3164..4774
                     /locus_tag="ACG8K3_000765"
     CDS             3164..4774
                     /locus_tag="ACG8K3_000765"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP9144668.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4751..4963)
                     /locus_tag="ACG8K3_000766"
     CDS             complement(4751..4963)
                     /locus_tag="ACG8K3_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144669.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5066..5935
                     /locus_tag="ACG8K3_000767"
     CDS             5066..5935
                     /locus_tag="ACG8K3_000767"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9144670.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ACG8K3_000768"
     CDS             6100..7371
                     /locus_tag="ACG8K3_000768"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9144671.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ACG8K3_000769"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ACG8K3_000769"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9144672.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ACG8K3_000770"
     CDS             8762..9025
                     /locus_tag="ACG8K3_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9144673.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ACG8K3_000771"
     CDS             9470..9919
                     /locus_tag="ACG8K3_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9144674.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ACG8K3_000772"
     CDS             9976..11394
                     /locus_tag="ACG8K3_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9144675.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ACG8K3_000773"
     CDS             11394..12254
                     /locus_tag="ACG8K3_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9144676.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ACG8K3_000774"
     CDS             12428..13201
                     /locus_tag="ACG8K3_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9144677.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ACG8K3_000775"
     CDS             13348..13713
                     /locus_tag="ACG8K3_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP9144678.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ACG8K3_000776"
     CDS             complement(13861..15069)
                     /locus_tag="ACG8K3_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9144679.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ACG8K3_000777"
     CDS             complement(15088..15366)
                     /locus_tag="ACG8K3_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144680.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="ACG8K3_000778"
     CDS             15559..16215
                     /locus_tag="ACG8K3_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9144681.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="ACG8K3_000779"
     CDS             complement(16657..17454)
                     /locus_tag="ACG8K3_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9144682.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ACG8K3_000780"
     CDS             complement(17451..18635)
                     /locus_tag="ACG8K3_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP9144683.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ACG8K3_000781"
     CDS             complement(18660..19907)
                     /locus_tag="ACG8K3_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9144684.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVCPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ACG8K3_000782"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ACG8K3_000782"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9144685.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ACG8K3_000783"
     CDS             21887..22201
                     /locus_tag="ACG8K3_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9144686.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ACG8K3_000784"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ACG8K3_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9144687.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /gene="tmk"
                     /locus_tag="ACG8K3_000785"
     CDS             22886..23530
                     /gene="tmk"
                     /locus_tag="ACG8K3_000785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9144688.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ACG8K3_000786"
     CDS             23545..23874
                     /locus_tag="ACG8K3_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP9144689.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ACG8K3_000787"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ACG8K3_000787"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9144690.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ACG8K3_000788"
     CDS             24862..25686
                     /locus_tag="ACG8K3_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP9144691.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ACG8K3_000789"
     CDS             25679..26026
                     /locus_tag="ACG8K3_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP9144692.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ACG8K3_000790"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ACG8K3_000790"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9144693.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ACG8K3_000791"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ACG8K3_000791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9144694.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ACG8K3_000792"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ACG8K3_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP9144695.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ACG8K3_000793"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ACG8K3_000793"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9144696.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIAEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31683..32450
                     /locus_tag="ACG8K3_000794"
     CDS             31683..32450
                     /locus_tag="ACG8K3_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144697.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ACG8K3_000795"
     CDS             32440..34482
                     /locus_tag="ACG8K3_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144698.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11116 a         6130 c         7259 g        10080 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgatttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagta gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga cagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccgcgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat ttttt
//
LOCUS       DAZKCN010000017        34397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000017 DAZKCN010000000
VERSION     DAZKCN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.017"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8K3_000796"
     CDS             352..1041
                     /locus_tag="ACG8K3_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9144699.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8K3_000797"
     CDS             1028..2236
                     /locus_tag="ACG8K3_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP9144700.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8K3_000798"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8K3_000798"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9144701.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8K3_000799"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8K3_000799"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9144702.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8K3_000800"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8K3_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP9144703.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8K3_000801"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8K3_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9144704.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8K3_000802"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8K3_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP9144705.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8K3_000803"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8K3_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9144706.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8K3_000804"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8K3_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9144707.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8K3_000805"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8K3_000805"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9144708.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8K3_000806"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8K3_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9144709.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8K3_000807"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8K3_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9144710.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8K3_000808"
     CDS             14660..15043
                     /locus_tag="ACG8K3_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144711.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8K3_000809"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8K3_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP9144712.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8K3_000810"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8K3_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP9144713.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8K3_000811"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8K3_000811"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9144714.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8K3_000812"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8K3_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9144715.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8K3_000813"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8K3_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9144716.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8K3_000814"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8K3_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9144717.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8K3_000815"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8K3_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP9144718.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8K3_000816"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8K3_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9144719.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8K3_000817"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8K3_000817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9144720.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8K3_000818"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8K3_000818"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP9144721.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8K3_000819"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8K3_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9144722.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8K3_000820"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8K3_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9144723.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8K3_000821"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8K3_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9144724.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG8K3_000822"
     CDS             28113..30086
                     /locus_tag="ACG8K3_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP9144725.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8K3_000823"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8K3_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9144726.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8K3_000824"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8K3_000824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9144727.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG8K3_000825"
     CDS             32965..34257
                     /locus_tag="ACG8K3_000825"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9144728.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6692 c         6807 g        10403 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAZKCN010000018        33549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000018 DAZKCN010000000
VERSION     DAZKCN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.018"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..1692
                     /locus_tag="ACG8K3_000826"
     CDS             31..1692
                     /locus_tag="ACG8K3_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9144729.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1762..2109)
                     /locus_tag="ACG8K3_000827"
     CDS             complement(1762..2109)
                     /locus_tag="ACG8K3_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP9144730.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2193..3134
                     /gene="opp3b"
                     /locus_tag="ACG8K3_000828"
     CDS             2193..3134
                     /gene="opp3b"
                     /locus_tag="ACG8K3_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9144731.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3135..4178
                     /locus_tag="ACG8K3_000829"
     CDS             3135..4178
                     /locus_tag="ACG8K3_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144732.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4184..5254
                     /locus_tag="ACG8K3_000830"
     CDS             4184..5254
                     /locus_tag="ACG8K3_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144733.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5244..6197
                     /locus_tag="ACG8K3_000831"
     CDS             5244..6197
                     /locus_tag="ACG8K3_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144734.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6209..7219
                     /locus_tag="ACG8K3_000832"
     CDS             6209..7219
                     /locus_tag="ACG8K3_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9144735.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7504..8325)
                     /locus_tag="ACG8K3_000833"
     CDS             complement(7504..8325)
                     /locus_tag="ACG8K3_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9144736.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8422..8700)
                     /locus_tag="ACG8K3_000834"
     CDS             complement(8422..8700)
                     /locus_tag="ACG8K3_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144737.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8968..10977
                     /gene="metG"
                     /locus_tag="ACG8K3_000835"
     CDS             8968..10977
                     /gene="metG"
                     /locus_tag="ACG8K3_000835"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9144738.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11456..12043)
                     /locus_tag="ACG8K3_000836"
     CDS             complement(11456..12043)
                     /locus_tag="ACG8K3_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144739.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12218..13540
                     /locus_tag="ACG8K3_000837"
     CDS             12218..13540
                     /locus_tag="ACG8K3_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9144740.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13695..14471
                     /locus_tag="ACG8K3_000838"
     CDS             13695..14471
                     /locus_tag="ACG8K3_000838"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9144741.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14464..15033
                     /gene="rnmV"
                     /locus_tag="ACG8K3_000839"
     CDS             14464..15033
                     /gene="rnmV"
                     /locus_tag="ACG8K3_000839"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP9144742.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15026..15910
                     /gene="rsmA"
                     /locus_tag="ACG8K3_000840"
     CDS             15026..15910
                     /gene="rsmA"
                     /locus_tag="ACG8K3_000840"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9144743.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16361..16990
                     /locus_tag="ACG8K3_000841"
     CDS             16361..16990
                     /locus_tag="ACG8K3_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144744.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17114..18223)
                     /locus_tag="ACG8K3_000842"
     CDS             complement(17114..18223)
                     /locus_tag="ACG8K3_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144745.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18432..18854
                     /gene="mgsA"
                     /locus_tag="ACG8K3_000843"
     CDS             18432..18854
                     /gene="mgsA"
                     /locus_tag="ACG8K3_000843"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9144746.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19041..19139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19177..19713
                     /locus_tag="ACG8K3_000844"
     CDS             19177..19713
                     /locus_tag="ACG8K3_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP9144747.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19753..21516)
                     /locus_tag="ACG8K3_000845"
     CDS             complement(19753..21516)
                     /locus_tag="ACG8K3_000845"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144748.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21519..23234)
                     /locus_tag="ACG8K3_000846"
     CDS             complement(21519..23234)
                     /locus_tag="ACG8K3_000846"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144749.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24013..24237)
                     /locus_tag="ACG8K3_000847"
     CDS             complement(24013..24237)
                     /locus_tag="ACG8K3_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144750.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24428..27205
                     /locus_tag="ACG8K3_000848"
     CDS             24428..27205
                     /locus_tag="ACG8K3_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9144751.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27286..27705
                     /locus_tag="ACG8K3_000849"
     CDS             27286..27705
                     /locus_tag="ACG8K3_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9144752.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27717..28187
                     /locus_tag="ACG8K3_000850"
     CDS             27717..28187
                     /locus_tag="ACG8K3_000850"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9144753.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28204..28983
                     /locus_tag="ACG8K3_000851"
     CDS             28204..28983
                     /locus_tag="ACG8K3_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9144754.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            28970..29791
                     /locus_tag="ACG8K3_000852"
     CDS             28970..29791
                     /locus_tag="ACG8K3_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9144755.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29808..30857
                     /locus_tag="ACG8K3_000853"
     CDS             29808..30857
                     /locus_tag="ACG8K3_000853"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9144756.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30870..31874
                     /locus_tag="ACG8K3_000854"
     CDS             30870..31874
                     /locus_tag="ACG8K3_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9144757.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32507..33526
                     /locus_tag="ACG8K3_000855"
     CDS             32507..33526
                     /locus_tag="ACG8K3_000855"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9144758.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        10942 a         5957 c         6874 g         9776 t
ORIGIN      
        1 tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt
       61 gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt
      121 tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa
      181 atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac
      241 gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc
      301 gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca
      361 aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca
      421 gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa
      481 gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat
      541 gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca
      601 tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca
      661 agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca
      721 gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt
      781 tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat
      841 ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca
      901 gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag
      961 aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa
     1021 gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca
     1081 gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt
     1141 tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt
     1201 gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa
     1261 tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt
     1321 ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga
     1381 tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca
     1441 tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca
     1501 acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc
     1561 tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa
     1621 aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac
     1681 gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg
     1741 caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag
     1801 agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga
     1861 gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg
     1921 ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa
     1981 cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat
     2041 gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt
     2101 ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc
     2161 tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa
     2221 aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga
     2281 tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg
     2341 agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc
     2401 tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg
     2461 cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct
     2521 ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg
     2581 cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg
     2641 ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg
     2701 atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact
     2761 ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag
     2821 caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt
     2881 tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg
     2941 tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa
     3001 caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg
     3061 ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag
     3121 ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg
     3181 cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac
     3241 cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta
     3301 tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc
     3361 ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa
     3421 tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg
     3481 ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg
     3541 atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg
     3601 cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac
     3661 gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg
     3721 tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg
     3781 ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag
     3841 atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca
     3901 aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat
     3961 ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag
     4021 cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat
     4081 tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg
     4141 gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga
     4201 agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg
     4261 tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg
     4321 aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc
     4381 tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa
     4441 aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac
     4501 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
     4561 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
     4621 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
     4681 gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga
     4741 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
     4801 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
     4861 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
     4921 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
     4981 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
     5041 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
     5101 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
     5161 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
     5221 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
     5281 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
     5341 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
     5401 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
     5461 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
     5521 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
     5581 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
     5641 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
     5701 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
     5761 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
     5821 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
     5881 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
     5941 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
     6001 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
     6061 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
     6121 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
     6181 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
     6241 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
     6301 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
     6361 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
     6421 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
     6481 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
     6541 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
     6601 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
     6661 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
     6721 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
     6781 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
     6841 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
     6901 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
     6961 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
     7021 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
     7081 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
     7141 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
     7201 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
     7261 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
     7321 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
     7381 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
     7441 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
     7501 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
     7561 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
     7621 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
     7681 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
     7741 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
     7801 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
     7861 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
     7921 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
     7981 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
     8041 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
     8101 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
     8161 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
     8221 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg
     8281 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
     8341 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
     8401 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
     8461 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
     8521 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
     8581 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
     8641 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
     8701 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
     8761 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
     8821 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
     8881 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
     8941 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
     9001 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
     9061 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
     9121 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
     9181 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
     9241 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
     9301 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
     9361 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
     9421 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
     9481 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
     9541 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
     9601 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
     9661 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
     9721 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
     9781 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
     9841 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
     9901 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
     9961 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
    10021 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
    10081 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
    10141 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
    10201 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
    10261 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
    10321 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
    10381 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
    10441 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
    10501 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
    10561 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
    10621 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
    10681 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
    10741 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
    10801 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
    10861 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
    10921 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
    10981 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
    11041 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
    11101 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
    11161 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
    11221 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
    11281 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
    11341 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
    11401 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
    11461 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
    11521 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
    11581 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
    11641 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
    11701 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
    11761 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
    11821 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
    11881 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
    11941 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
    12001 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
    12061 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
    12121 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
    12181 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
    12241 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
    12301 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
    12361 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
    12421 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
    12481 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
    12541 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
    12601 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
    12661 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
    12721 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
    12781 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
    12841 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
    12901 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
    12961 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
    13021 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
    13081 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
    13141 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
    13201 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
    13261 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
    13321 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
    13381 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
    13441 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
    13501 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    13561 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    13621 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    13681 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    13741 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    13801 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    13861 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    13921 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    13981 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    14041 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    14101 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    14161 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    14221 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    14281 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    14341 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    14401 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    14461 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    14521 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    14581 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    14641 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    14701 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    14761 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    14821 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    14881 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    14941 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    15001 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    15061 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    15121 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    15181 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    15241 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    15301 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    15361 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    15421 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    15481 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    15541 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    15601 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    15661 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    15721 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    15781 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    15841 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    15901 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    15961 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
    16021 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    16081 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    16141 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    16201 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
    16261 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
    16321 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
    16381 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
    16441 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
    16501 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
    16561 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
    16621 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
    16681 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
    16741 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
    16801 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
    16861 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
    16921 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
    16981 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
    17041 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
    17101 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
    17161 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
    17221 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
    17281 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
    17341 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
    17401 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
    17461 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
    17521 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
    17581 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
    17641 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
    17701 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
    17761 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
    17821 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
    17881 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
    17941 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
    18001 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
    18061 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
    18121 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
    18181 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
    18241 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
    18301 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
    18361 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
    18421 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
    18481 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
    18541 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
    18601 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
    18661 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
    18721 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
    18781 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
    18841 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
    18901 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
    18961 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
    19021 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
    19081 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
    19141 gatacgatgt agcagctctt aaaggagatg ctaaaaataa agaaacaccg gttagacgca
    19201 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
    19261 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
    19321 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
    19381 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
    19441 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
    19501 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
    19561 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
    19621 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
    19681 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
    19741 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
    19801 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
    19861 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
    19921 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
    19981 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
    20041 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
    20101 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
    20161 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
    20221 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
    20281 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
    20341 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
    20401 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
    20461 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
    20521 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
    20581 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
    20641 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
    20701 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
    20761 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
    20821 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
    20881 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
    20941 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
    21001 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
    21061 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
    21121 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
    21181 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
    21241 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
    21301 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    21361 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    21421 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    21481 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    21541 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    21601 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    21661 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    21721 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    21781 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    21841 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    21901 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    21961 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    22021 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    22081 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    22141 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    22201 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    22261 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    22321 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    22381 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    22441 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    22501 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    22561 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    22621 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    22681 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    22741 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    22801 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    22861 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    22921 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    22981 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    23041 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    23101 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    23161 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    23221 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    23281 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    23341 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    23401 atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta atttttctat
    23461 aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact
    23521 tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt
    23581 gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt
    23641 ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca
    23701 gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg
    23761 agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg
    23821 ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg
    23881 acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat
    23941 aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga
    24001 aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat
    24061 gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc
    24121 tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat
    24181 ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga
    24241 atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa
    24301 ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct ataataagat
    24361 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
    24421 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
    24481 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
    24541 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
    24601 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
    24661 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
    24721 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
    24781 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
    24841 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
    24901 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
    24961 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
    25021 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
    25081 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
    25141 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
    25201 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
    25261 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
    25321 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
    25381 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
    25441 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
    25501 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
    25561 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
    25621 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
    25681 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
    25741 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
    25801 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
    25861 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
    25921 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
    25981 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
    26041 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
    26101 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
    26161 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
    26221 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
    26281 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
    26341 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
    26401 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
    26461 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
    26521 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
    26581 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
    26641 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
    26701 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
    26761 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
    26821 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
    26881 agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
    26941 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
    27001 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
    27061 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
    27121 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
    27181 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
    27241 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
    27301 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
    27361 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
    27421 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
    27481 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
    27541 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
    27601 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
    27661 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
    27721 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
    27781 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
    27841 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
    27901 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
    27961 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
    28021 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
    28081 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
    28141 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
    28201 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
    28261 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
    28321 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
    28381 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
    28441 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
    28501 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
    28561 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
    28621 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
    28681 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
    28741 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
    28801 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
    28861 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
    28921 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
    28981 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
    29041 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
    29101 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
    29161 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
    29221 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
    29281 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
    29341 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
    29401 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
    29461 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
    29521 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
    29581 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
    29641 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
    29701 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
    29761 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
    29821 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
    29881 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
    29941 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
    30001 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
    30061 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
    30121 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
    30181 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
    30241 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
    30301 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
    30361 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
    30421 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
    30481 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
    30541 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
    30601 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
    30661 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
    30721 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
    30781 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
    30841 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
    30901 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
    30961 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
    31021 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
    31081 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
    31141 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
    31201 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
    31261 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
    31321 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
    31381 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
    31441 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
    31501 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
    31561 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
    31621 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
    31681 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
    31741 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
    31801 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
    31861 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
    31921 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
    31981 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
    32041 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
    32101 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
    32161 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
    32221 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
    32281 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
    32341 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
    32401 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
    32461 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
    32521 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
    32581 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
    32641 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
    32701 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
    32761 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
    32821 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
    32881 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
    32941 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
    33001 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
    33061 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
    33121 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
    33181 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
    33241 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
    33301 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
    33361 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
    33421 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
    33481 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
    33541 gttaatcgt
//
LOCUS       DAZKCN010000019        33269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000019 DAZKCN010000000
VERSION     DAZKCN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.019"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..233)
                     /gene="rpmB"
                     /locus_tag="ACG8K3_000856"
     CDS             complement(45..233)
                     /gene="rpmB"
                     /locus_tag="ACG8K3_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9144759.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            503..865
                     /locus_tag="ACG8K3_000857"
     CDS             503..865
                     /locus_tag="ACG8K3_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9144760.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            917..2599
                     /locus_tag="ACG8K3_000858"
     CDS             917..2599
                     /locus_tag="ACG8K3_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP9144761.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2716..4752
                     /gene="recG"
                     /locus_tag="ACG8K3_000859"
     CDS             2716..4752
                     /gene="recG"
                     /locus_tag="ACG8K3_000859"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9144762.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4801..5802
                     /gene="plsX"
                     /locus_tag="ACG8K3_000860"
     CDS             4801..5802
                     /gene="plsX"
                     /locus_tag="ACG8K3_000860"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9144763.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5924..6166
                     /gene="acpP"
                     /locus_tag="ACG8K3_000861"
     CDS             5924..6166
                     /gene="acpP"
                     /locus_tag="ACG8K3_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9144764.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6434..7438
                     /locus_tag="ACG8K3_000862"
     CDS             6434..7438
                     /locus_tag="ACG8K3_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144765.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7439..8383
                     /locus_tag="ACG8K3_000863"
     CDS             7439..8383
                     /locus_tag="ACG8K3_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144766.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8383..9345
                     /gene="opp4B"
                     /locus_tag="ACG8K3_000864"
     CDS             8383..9345
                     /gene="opp4B"
                     /locus_tag="ACG8K3_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP9144767.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9372..10286
                     /locus_tag="ACG8K3_000865"
     CDS             9372..10286
                     /locus_tag="ACG8K3_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144768.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10312..12093
                     /locus_tag="ACG8K3_000866"
     CDS             10312..12093
                     /locus_tag="ACG8K3_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9144769.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12441..13127
                     /gene="rnc"
                     /locus_tag="ACG8K3_000867"
     CDS             12441..13127
                     /gene="rnc"
                     /locus_tag="ACG8K3_000867"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9144770.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13147..16728
                     /gene="smc"
                     /locus_tag="ACG8K3_000868"
     CDS             13147..16728
                     /gene="smc"
                     /locus_tag="ACG8K3_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP9144771.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16737..17546
                     /locus_tag="ACG8K3_000869"
     CDS             16737..17546
                     /locus_tag="ACG8K3_000869"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9144772.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17557..18555
                     /gene="ftsY"
                     /locus_tag="ACG8K3_000870"
     CDS             17557..18555
                     /gene="ftsY"
                     /locus_tag="ACG8K3_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9144773.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18742..21009
                     /gene="gshAB"
                     /locus_tag="ACG8K3_000871"
     CDS             18742..21009
                     /gene="gshAB"
                     /locus_tag="ACG8K3_000871"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP9144774.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21098..21550)
                     /locus_tag="ACG8K3_000872"
     CDS             complement(21098..21550)
                     /locus_tag="ACG8K3_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP9144775.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21700..22650
                     /locus_tag="ACG8K3_000873"
     CDS             21700..22650
                     /locus_tag="ACG8K3_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9144776.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22643..23602
                     /locus_tag="ACG8K3_000874"
     CDS             22643..23602
                     /locus_tag="ACG8K3_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9144777.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23599..24354
                     /locus_tag="ACG8K3_000875"
     CDS             23599..24354
                     /locus_tag="ACG8K3_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144778.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24377..25330
                     /locus_tag="ACG8K3_000876"
     CDS             24377..25330
                     /locus_tag="ACG8K3_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9144779.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25974..26270)
                     /locus_tag="ACG8K3_000877"
     CDS             complement(25974..26270)
                     /locus_tag="ACG8K3_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP9144780.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26625..26981
                     /locus_tag="ACG8K3_000878"
     CDS             26625..26981
                     /locus_tag="ACG8K3_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9144781.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26991..27890
                     /locus_tag="ACG8K3_000879"
     CDS             26991..27890
                     /locus_tag="ACG8K3_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9144782.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28410..29138
                     /locus_tag="ACG8K3_000880"
     CDS             28410..29138
                     /locus_tag="ACG8K3_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9144783.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29283..31310
                     /locus_tag="ACG8K3_000881"
     CDS             29283..31310
                     /locus_tag="ACG8K3_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP9144784.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            31418..32515
                     /locus_tag="ACG8K3_000882"
     CDS             31418..32515
                     /locus_tag="ACG8K3_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP9144785.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32822..>33269
                     /locus_tag="ACG8K3_000883"
     CDS             32822..>33269
                     /locus_tag="ACG8K3_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9144786.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        11143 a         5759 c         7124 g         9243 t
ORIGIN      
        1 aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact
       61 tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata
      121 acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa
      181 cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt
      241 tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc
      301 atatagaatg ggaatttgca agtacatttt catagataca attgacaatt gagaagaata
      361 attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa
      421 ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt
      481 tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga
      541 aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt
      601 tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga
      661 aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta
      721 tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt
      781 gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca
      841 aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg
      901 aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt
      961 ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt
     1021 ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa
     1081 ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg
     1141 attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt
     1201 ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac
     1261 tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac
     1321 agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt
     1381 tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt
     1441 gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt
     1501 ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc
     1561 ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca
     1621 aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga
     1681 aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat
     1741 aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga
     1801 aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt
     1861 cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt
     1921 tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg
     1981 cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat
     2041 gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat
     2101 tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga
     2161 tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc
     2221 attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag
     2281 cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat
     2341 caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat
     2401 cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac
     2461 gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac
     2521 agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta
     2581 tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat
     2641 aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg
     2701 agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga
     2761 gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact
     2821 tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa
     2881 gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat
     2941 aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt
     3001 tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt
     3061 aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg
     3121 gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat
     3181 ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac
     3241 cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca
     3301 aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta
     3361 tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag
     3421 atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca
     3481 aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg
     3541 cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta
     3601 tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca
     3661 caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg
     3721 attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg
     3781 gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat
     3841 ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt
     3901 cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca
     3961 ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga
     4021 cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg
     4081 acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa
     4141 tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt
     4201 gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta
     4261 atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa
     4321 gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga
     4381 ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt
     4441 atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag
     4501 accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta
     4561 tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga
     4621 aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag
     4681 atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt
     4741 tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca
     4801 atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc
     4861 gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca
     4921 gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa
     4981 atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc
     5041 cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca
     5101 ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct
     5161 ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat
     5221 cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct
     5281 ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga
     5341 acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa
     5401 acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat
     5461 gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca
     5521 atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg
     5581 ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa
     5641 catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca
     5701 ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta
     5761 gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc
     5821 gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg
     5881 gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt
     5941 atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac
     6001 ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt
     6061 tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga
     6121 aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc
     6181 aaagaaggat aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac
     6241 gtaaaagtca tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc
     6301 ggttttctat ataattatta ataattcatt acacaaagat aagaataacc tttgttataa
     6361 aacgatttat tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga
     6421 gaggggtatt cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt
     6481 ccgactcaaa gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa
     6541 cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat
     6601 tatgggatta cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct
     6661 gaatttaatc gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat
     6721 gattttccaa gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa
     6781 agaaggactt tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga
     6841 attgctagaa caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga
     6901 actatcaggc ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc
     6961 aatcgtcatt gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct
     7021 tgatttactg aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga
     7081 cttaggagtc gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt
     7141 tgaagtagca ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt
     7201 actgaactct ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt
     7261 cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg
     7321 gataccagca agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt
     7381 ggtacgttgc tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat
     7441 gacagaatta atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt
     7501 caatcgagta acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg
     7561 aaagacatat ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt
     7621 tgtgggatta gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa
     7681 gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc
     7741 aatgtccagt ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa
     7801 ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt
     7861 tgggatgccg gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca
     7921 acgtctagga gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc
     7981 tgtatctgct ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca
     8041 gcaagaatac ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat
     8101 gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga
     8161 catttataat gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat
     8221 cgatgtagaa aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat
     8281 ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac
     8341 aactacacac aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg
     8401 cgacgtgttt tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta
     8461 gcccaagcga tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca
     8521 gtgatcgaac agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc
     8581 cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct
     8641 gttgctgact tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt
     8701 gtccttactt atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca
     8761 atcttggata agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc
     8821 ttcggcttga tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga
     8881 tcacaaacac taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat
     8941 attctgttac catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg
     9001 cgaagtgaag taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg
     9061 gtaccgacga ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct
     9121 tctcaaatgg ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt
     9181 tttggttatc cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc
     9241 gtgatcacat ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca
     9301 gatatcatca tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg
     9361 agaatggatc gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg
     9421 ggatacgaat gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt
     9481 tgctagtggt tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag
     9541 tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac
     9601 aaaagttcat tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag
     9661 gtgctagaaa ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg
     9721 ttggattagg gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga
     9781 tcgttgattt tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta
     9841 tttccagata caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta
     9901 aagcccgatt attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct
     9961 caaaaacaat gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa
    10021 gttcattaat cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa
    10081 ctttgacttt cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg
    10141 gttatgcaag taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa
    10201 tccttatttt agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg
    10261 cagatgcgcg ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa
    10321 acgatttttg gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga
    10381 ggtacagata cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact
    10441 tttccaattg caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg
    10501 gtcgcaacgg acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat
    10561 gatgcagctt ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga
    10621 atcacagacg acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc
    10681 aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt
    10741 ttcccttacg aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc
    10801 cgtaacatcg taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc
    10861 aaaaaaatag acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt
    10921 caatcgggtg gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca
    10981 atcaaagata tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat
    11041 tacatgagta atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg
    11101 aatggtacgc ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact
    11161 gaagcattga actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat
    11221 acctataaag aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat
    11281 ctaggattca agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat
    11341 tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca
    11401 gtaggtgaac gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta
    11461 ttccagtcat accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa
    11521 aaactattag atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa
    11581 gatggaaata aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca
    11641 ttggcagatt actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca
    11701 ggtcgtctga tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt
    11761 gatgtttacc aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga
    11821 ccaaatgctg cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac
    11881 gatatcagtt caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg
    11941 caacaatatg catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg
    12001 ccagtcaacg accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca
    12061 aatgtttctg ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga
    12121 tgtccttttt cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg
    12181 ctgatttcat gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca
    12241 aatttatgca aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta
    12301 aacgaatttt tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt
    12361 gaccgaacca tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat
    12421 ccataaaagg aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata
    12481 acatcgtttt tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga
    12541 atgagcatcg caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg
    12601 ttttagaact gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa
    12661 aattgacaaa aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag
    12721 aatgtcactt tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta
    12781 cgcgaccagc attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc
    12841 agggattcga cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag
    12901 gtgctttttc acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag
    12961 gtgatgtatc aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat
    13021 tttggatcga agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa
    13081 aattagcaga acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg
    13141 aaagctttgt atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca
    13201 gtcattgatt ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc
    13261 aatattacag aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc
    13321 ggaaaaatgc cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca
    13381 gaagtcacag tgattttaga caatagtgat cattatctgc cattggagta caatgagatc
    13441 agtgtaaccc gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc
    13501 tgcagattaa aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc
    13561 tcaatcattt ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt
    13621 gggatctttg aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa
    13681 cagaagcttt ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg
    13741 gaagaacagt taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta
    13801 aaagaaacac tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa
    13861 aaagattggg acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta
    13921 tctgagtcga tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa
    13981 gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag
    14041 cagacagaag gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg
    14101 caagaatatc aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt
    14161 caagaaagct tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca
    14221 aatctcataa aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg
    14281 gcagaattaa gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa
    14341 ctgaagtatc tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc
    14401 aagcaatcgg aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca
    14461 caaaagcagg cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc
    14521 caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa
    14581 ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa
    14641 aactattttg gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg
    14701 ggtattgtcg gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc
    14761 gaaacagctt taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg
    14821 caagccatca cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg
    14881 acaatcaaac caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg
    14941 tttataggga ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa
    15001 aacctgctag gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa
    15061 acgatccgct atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga
    15121 tccatgacag gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa
    15181 gaattgaaac agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa
    15241 aaaaaagttc aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg
    15301 cgtacacgag gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac
    15361 atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt
    15421 gaactgcagc agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa
    15481 acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa
    15541 agcgaccaga tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca
    15601 gatcttgctg tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga
    15661 gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg
    15721 acagcagact tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa
    15781 ttatctgctc agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat
    15841 agaaggcaaa aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa
    15901 aaagcacgac tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt
    15961 ttagataatc atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc
    16021 caagactatc aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa
    16081 gaacagattg aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc
    16141 agtgaaagac atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa
    16201 ttgtttgaaa cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt
    16261 gaagctatcc gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct
    16321 gaattagtac tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa
    16381 cctcctggca aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca
    16441 gccatcgcgt tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat
    16501 gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat
    16561 ttccaagatg acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca
    16621 gatgtactgt acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg
    16681 atggaagata tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga
    16741 tcaaattagt agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag
    16801 atgagaataa acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta
    16861 caggacgccc cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag
    16921 gtgactacag catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca
    16981 tggaaaagaa agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat
    17041 tggatctgcc actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa
    17101 ggaagcaatc cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa
    17161 gcgatattac agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat
    17221 tagatcctaa aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa
    17281 caagaagtat gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt
    17341 tattggcaaa agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa
    17401 atgatttacc gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt
    17461 tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag
    17521 tgatccaaaa atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag
    17581 cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag
    17641 ggtttagaaa agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt
    17701 cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta
    17761 ggttttgaaa cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat
    17821 gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa
    17881 gcggaaggaa tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt
    17941 ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag
    18001 ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca
    18061 atcgaccagt tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca
    18121 ggaggcgatc cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca
    18181 gatgttttac tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa
    18241 ttagaaaaaa taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt
    18301 cttgtattag atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa
    18361 acaacagatg tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc
    18421 gtacttgcta tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga
    18481 atcgatgact tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta
    18541 ctaaaagaag aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt
    18601 gtttgaatga ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt
    18661 gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc
    18721 atatgaattg gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg
    18781 cgccctttta tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt
    18841 gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc
    18901 tatatccaaa cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct
    18961 attcccgaat tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg
    19021 aaagaagaga tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag
    19081 atcattattg caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa
    19141 gaatacggga agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat
    19201 gagttgctaa gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa
    19261 acagaacttt atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat
    19321 ttattcggtg cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa
    19381 gaacctgttc gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa
    19441 gtttcctatg cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg
    19501 aaactttcgg aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc
    19561 gcagatttag caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct
    19621 tatgcaagat tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac
    19681 atgttatgga cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga
    19741 aataacaaag tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga
    19801 gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat
    19861 ttagtagagg aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc
    19921 tatcaagaag cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat
    19981 tataaagaaa gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct
    20041 actcagatat ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag
    20101 tcggatcaat ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac
    20161 atgacaagta aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa
    20221 aaagtcttaa aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa
    20281 gaagcggtaa aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca
    20341 accaattacg ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa
    20401 gcaggtttag ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg
    20461 ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc
    20521 cctgctaatg ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat
    20581 agtgaccctt tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa
    20641 ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat
    20701 caaattgttt atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg
    20761 actgatgaat tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga
    20821 gctaagatta gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat
    20881 aaaaaagcat atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat
    20941 ccttttgcag gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa
    21001 gtattttaaa agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag
    21061 ctttttttgc atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc
    21121 tttttttctt ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt
    21181 tttcgatatc aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt
    21241 cctcaggatt tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg
    21301 ctagctggat atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag
    21361 catctggatg ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt
    21421 cagcaacagt catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt
    21481 cttcagggtt cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat
    21541 cttgtgccat aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt
    21601 cccaataata cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa
    21661 taagaatgaa aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta
    21721 gcattattag ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg
    21781 tcagaactgc tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca
    21841 cgaacgattt gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa
    21901 catttgaccc aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt
    21961 ttaggtattt tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg
    22021 acagcatttt gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc
    22081 ctccagaaaa atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt
    22141 tcagttgcta ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt
    22201 caagggaatt ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca
    22261 ttgttgctct tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg
    22321 tttgcagtaa atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta
    22381 tcattggcta gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc
    22441 attccgaatc ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact
    22501 gctttagcag gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt
    22561 ccttatgaaa taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat
    22621 cttctgataa ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct
    22681 tgttcttgtt ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca
    22741 ttttgcatgt gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt
    22801 cagcagtgcc acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt
    22861 attgggactt gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg
    22921 agtgacgatg cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt
    22981 cgggctgagt ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt
    23041 attactgatg tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca
    23101 agtcatcatg gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg
    23161 caatatccag agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct
    23221 gttatttcga tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa
    23281 cctcgggata gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac
    23341 agggacatca acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat
    23401 cagttatcgt gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt
    23461 agctatgctg tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac
    23521 aacagtgagt gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg
    23581 cgaaaggaag agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa
    23641 tcagtggtta aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt
    23701 ccaaatggtg ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat
    23761 acaggtgaaa tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc
    23821 aaaaaattat cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa
    23881 ttagtagaat ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa
    23941 atcgttcagg aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac
    24001 actctttcag gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg
    24061 gattatattt tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc
    24121 atgcagattt taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat
    24181 gatatcaact ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta
    24241 tttacgcaag gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa
    24301 atggacatac ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa
    24361 cattaggaga gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg
    24421 tttagcagca tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac
    24481 acaaactgaa actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa
    24541 taatccagaa aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg
    24601 tgttggagat aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta
    24661 taaaaaagta gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct
    24721 tcagccagat ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca
    24781 aattgctccg accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca
    24841 aaatgtcact gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga
    24901 ggaattatct agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc
    24961 actagtcaca cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg
    25021 tttcatccat gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca
    25081 cggtcaaagt gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt
    25141 agacagaaca aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact
    25201 ggtagcacaa acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg
    25261 gtatttaagc gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc
    25321 atttaaatag cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct
    25381 tacattctta aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat
    25441 cgtattgatg attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat
    25501 tcattatcta catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc
    25561 cattttttca gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt
    25621 agataaaact taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc
    25681 catctgctgc ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg
    25741 tccagcgttc ttattactca aagcaaatcg cagttgtcac aatctcttct aaacgatgtt
    25801 ccatctgctg cttctcggca ataagcccac tagactagaa aatatgagaa gctattttct
    25861 gtccagcgtt cttattactc aaagcaaatc gcagttgtca caatctcttc taaacaatgt
    25921 tcaatcagct gaccttcggt attagctcaa agcactgatt tcacaacctc tatttaggca
    25981 tgtgtctcag caagcggttg atagttgatg atctgcattt ttcgataaaa atcggcggga
    26041 agcttactcc accgttttaa taattgatag aaataactct cttctgcttt gattttatac
    26101 tcgcttaata tcaatccaaa actattcgtt ttttttgaag ctaatttttt ataaatatcc
    26161 aatgctttgg ttttatcttc attgacaaag agtgttttta cttcaatata agaaccctcg
    26221 atattttgtg tttcttcgat aaaatagatg ttttttatat attccttcat agtgattcct
    26281 ccaaagaagt cttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa
    26341 caaaaagtaa ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt
    26401 gttatttaag aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg
    26461 ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa
    26521 taaacgaatg acttactttc caataaattg agaacagtca gaaccagaca aattagttgc
    26581 atctgatgaa taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga
    26641 caaaagaagc acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag
    26701 atatcgaaaa cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggatacgg
    26761 catttcgatt gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag
    26821 tggctgatac accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg
    26881 atccatccac acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg
    26941 gcttgagaaa aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat
    27001 tggcgatcat cagcgaccta catgtggaca tcaataaatt cggcgaatca gagctgatgc
    27061 agctttggca agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg
    27121 ctaataaagt agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta
    27181 cgacatttca tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc
    27241 attttccaga cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc
    27301 ttttagggat gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa
    27361 tcgtacgaat gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc
    27421 tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa
    27481 aaaaacttga ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc
    27541 aatctgctcc ttatgagcgt tggaacaaat taaatgcatt tttaggatca gaatcattcg
    27601 gagaattact tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc
    27661 gctttgaaga taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt
    27721 atgaatggca gctgacccga gattttgtct tgaaagaaca gttgctagac agctatgatc
    27781 ctatgaaatt acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac
    27841 aacatttgca agaggaattt gaacaagcga tgacaatcgt tccgtattga gacagcatta
    27901 gtaatgtgta aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat
    27961 ttatcgtgga tatgctgctt tttttgcgga aaataatcag cgaatgttta tttgatcgga
    28021 tgatttgtaa ctcgcagagt actagatgca agcgtgtatt ctttttttga acgataatca
    28081 caaaaaaaaa tataatatac attttttatt taattataat taatattgat tgaagtctag
    28141 gcagtcaagt cacattttga tagataggag ggggacttac gaattatttt aaaggctatg
    28201 acagctgtat ttctagattg tgtttgcatc aagttgcaat aaaaactaga aatcaaatcg
    28261 ggctgataat catttataac taatcgatac ataggaccaa atataggtct atgttctttt
    28321 atttcataag tatcgtaaaa cagaattttg ctatggatta atccaatcga ctgatatgtt
    28381 tagtaaataa agaaagagga atgtcaatga tgaaaaaaga aatcgtatta acagcagcaa
    28441 tgatgttatt cagcatggta gcaagtacga cttttgtttc tgcaactgaa gtatacccaa
    28501 aggaatataa cacagaagga acgattacat ttgaagcagg agatgagggt gtaactcctc
    28561 ctgttgatcc agagaatcct gatcccaata aacctgtcga tccatcagat cctccaagcc
    28621 ctggaacagg aggggcatta tcgattgatt atggatcaaa attcaaattt gggactcaaa
    28681 aaatttctac tgcggataaa acgtattatg cagctgctga tgtgatgaac gatgggtcta
    28741 gaaaaccgac atatgtccaa gtgacagata ggagaagtac cttatctggg tggaaattaa
    28801 gtgtctctca accagaacag tttaaaactg ccagtgggga tgaactagtt ggagcacagc
    28861 tgaaatttac gaaagggcag gctgtttctt tggttgatcc aacatatacg ccgcaaacgg
    28921 tgaattctga attaacgttg acaccgggag gaaacaatac gttagcaatt aatgctaaat
    28981 caggaacagg tgtggggact tgggtctatc gatttggtgc aaatgaaaat gaaaatcaag
    29041 atgcggtaca gctttcggta cccggtaaat ctgtcaagtt agcgcaacaa tattccacta
    29101 aattagtttg gacattagaa gatacaccta acaactagtt tactgaaaag aactgttaaa
    29161 aataaatacg tagtcatatt gtcttgcgag gtaatcgagc aaaaattgtc aaaggctagg
    29221 ctaaatttgt ttggatacga atagttcatc taggaattta acgatcagga ggaaaagata
    29281 tattgaaaaa gaggaggatc gtgctcattt taagcagtct cttagtgact aatatctttc
    29341 cgatcatagg gtactctttg gaattgaatc agttggagca tggaaataga agagacgaag
    29401 aaagcaaact cacttcgaca ttcgacagtt taatagaatc caaccagaca caagaaagca
    29461 gtgaggattt aagtatttcg agtagtggag agaccactga caacgtagaa gctgagggtt
    29521 cggacaaaca aggactagaa gatgcaccaa tttctgcggt tgagctgcct gacgatccca
    29581 atattatttc tatcgataaa atttttcaaa cacctatcgg tactagcacg tccatattgg
    29641 aaaatgggaa gttactgcag ttgaacccag cagaaaggtc tcaaagagga gctatttggt
    29701 caaataagaa gattagttta ctctcggatt ttacatttaa atcttattta tacttaggaa
    29761 atcaacgaga tagagcggga gatggcatga cctttacatt gactggcgat cctagaatgg
    29821 ctagtaatcc cagtcaagtt attggatcgc caggtatggg aattggctca tattctactc
    29881 gttctggcca accttacatt gcgaatgcgc tctcgattga gtttgatacg tataaaaata
    29941 gtggttcttc tgatcgaatg gatcgagaga taactgctga taatagaggg catggacatg
    30001 tcgcttttgt cacgcctaaa ccaaataata ataattattc tggagagcat actggcgtga
    30061 cggtagctcc tacatttctt tctaatggca catggcgctt gttaataatt aaatgggacg
    30121 cggagcaaag aagtcttact tatgatttgg aaggtgtcgg tcactcttct cggattatac
    30181 ctgatctcat taggcagttt ggttctacag aagtaaattg ggggtttact tcttcaacag
    30241 gtactttttt tcaagaaaat gctgtcgctg tcacgcgact tccatcgaat gtcacatcaa
    30301 cagctgatgt aaaagtcaac gatagtgatt atggggccat cgctgaagct ggaggaaacg
    30361 ataagataac tatcagaaat acattgaagg tcgaggggga ttttcttgaa tataagaaaa
    30421 tgcagactag tatcgacctc ccacaagaat taactattcc cgagcatatc ttgatcaatg
    30481 gaaatcaaat tgatgccaaa gatatcttga taaccggaaa tcgagtcacg ctaaatctga
    30541 cagattattt tattgctgga gatacgttaa taatcgaatt ggagacaact ttgcaggaag
    30601 taattcctga aaaagtactc actatgcatt ttgaatacct tgaaaacggg aatttgattg
    30661 agcaatcgaa tgaaataaac ctaaagatcg caaagctgaa ggagatagat atccatgttt
    30721 attataaaga taaagacagt cagattgata ttgctgaaat gaaaacaatc actgggaaaa
    30781 taggagagaa gtataaagaa actcctattg atattgaagg gtatgttttt gactcggatt
    30841 cagcgaatac agaaggcatc ttctccgaaa atacagagga tatctatttt tattacagaa
    30901 aaggcaaact ttatctctca actgctcctc aatatgtcaa ttttggaaaa cataaaatat
    30961 ccagtagacc attaagtgaa ttcggacagt tttcaggtgg gttgaaaatt atagatgaac
    31021 gggcttttgg aagttggaaa ttacaattga aacaagagaa agttcttaca aatggagatt
    31081 ttgagcttcc agaagctctt tcctttgtta cctctgaaaa tacaacggtt atagggacat
    31141 ccgctgttac gattttcgaa agtgatcaaa agggtgaaag cgatttgtcc tctcttttag
    31201 attctacagc tcatcgtgga atcagaatag atattcctgt agaaaaccaa agaatcggaa
    31261 cctttgaggg gaaactatct tggatacttg cagatgtccc aggaaattaa caggaagaga
    31321 agaatataac aaaattattg agtttcaaga agggcacagg taccataggc tcttgtgtcc
    31381 cacagagaca agattttgtt atagtcttct cttacatatg attaaaggag cgattttctt
    31441 gagaccaaaa caacaattat ttataacact cctagttatt ttaggggcac tttttaacaa
    31501 tatatcagtg tcagctagtg catttaattt ttcagtgacg ccgataactt cagagaatca
    31561 aatcgacaag agaaaaactt acttcgatct tcaacttgat ccggatcagg aagtagaagt
    31621 aaaagcggaa ttgagaaatg atacagaaaa agaagtgaaa atcgatattt ctgtgaatag
    31681 cgcaaccacc aattcaaatg tgatggttga atacggaaaa aacgagattg aaaaagacat
    31741 gagcctgatc tttgatctgg tagattatgt gagctatcca cagatagtga cgctaaaacc
    31801 taaaagtgtc caaaccgttg cttttcatgt caggatgccg aatgagcgat ttgatggtgt
    31861 attggctggt gggatcacat tccaagaaat ccagacggaa aaggaccaaa cagaaactaa
    31921 ggatcaaagc ttatcgattg agaatgaata cacctatatt attgctttgt tgatgcaaca
    31981 gacactgaac gaagttgctc cgaaccttac tcttcatgaa gttaaaccag atcaaatcaa
    32041 tgcaagaaat gtcatcttag caaacgttca aaatgatcaa aaaacctata tcaatcaagt
    32101 agttattgaa acaaaaatta caaaaaaagg acattctgaa gttttatatc aagaggaaaa
    32161 agaaggattg cagattgcgc cgaatacaaa tttttctttt ccaactgctt taaatggtca
    32221 accgctaacc ccaggtgaat atcaccttac aatgactgtt ttaggaaatg aaaatgaaaa
    32281 tggaaaattt tcgagaaaaa aaggaaatac tacgatcaac tataccaatc aatgggtatt
    32341 tgaaaaagat tttacaatag acggaaaagt agccaaggaa ttgaacacga cggatgttac
    32401 gattaaaaaa gacaataatt ggatttatat cttagtagct tcacttttct tattgtttat
    32461 tttattatgc atacttttgt ttgttttgag aaaaaaagaa gagaaagaaa agtaaggtta
    32521 agataacaaa tagtaaattt ttattattgt aggatggttg ggctcttttt ataacaatat
    32581 aaaatatacc attttattaa taaaaatgca tactagtaaa tgatataatt ctaataacag
    32641 gtttgatcgc gagaaaggcc aaataaaacg ctctccaaaa tgttaaaaaa aagacaggac
    32701 gatgtatatt atacaaacaa gaatcgctga caacagtgac tcggatccag gaaatcagtt
    32761 ttatccccag ctagttggtg aatgcatgga aagtaatata cgtagaaaga gctgatgact
    32821 aatgaattat gagttttttt taagcaaaga agatcacaga agttattcat tacttaaata
    32881 tttagaagca agctcaaatt tatcagagtc aatatcgaat gttcaagaag agttgtcgct
    32941 atcgacattt ctcttgaaaa aaacgatcga taagctgaaa catgatttac aaaagttcga
    33001 tctagagaaa aatttgcgag taaccgtcag tgaattggat atatgtctgg aaattaatgg
    33061 aaaatgttct agcaaagctc tgttatctcg ttatataacg gattctttat ctatgaaaat
    33121 ggtattggct ttctttcagg aaaaatatat ctcagtagaa aattttgcta aacagcatca
    33181 cgtgagttat tctgtggctt ataaagtatt acaaagacta aaaagaaatc taaaaaaata
    33241 tcaaattttt tttgaaaaga gaaagttga
//
LOCUS       DAZKCN010000020        33071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000020 DAZKCN010000000
VERSION     DAZKCN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.020"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..185)
                     /locus_tag="ACG8K3_000884"
     CDS             complement(<1..185)
                     /locus_tag="ACG8K3_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144787.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(332..667)
                     /locus_tag="ACG8K3_000885"
                     /pseudo
     CDS             complement(332..667)
                     /locus_tag="ACG8K3_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(679..2271)
                     /gene="mobP2"
                     /locus_tag="ACG8K3_000886"
     CDS             complement(679..2271)
                     /gene="mobP2"
                     /locus_tag="ACG8K3_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFP9144788.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYVVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKNLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2291..2713)
                     /locus_tag="ACG8K3_000887"
     CDS             complement(2291..2713)
                     /locus_tag="ACG8K3_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144789.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            complement(2755..3030)
                     /locus_tag="ACG8K3_000888"
     CDS             complement(2755..3030)
                     /locus_tag="ACG8K3_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144790.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            complement(3793..4911)
                     /locus_tag="ACG8K3_000889"
     CDS             complement(3793..4911)
                     /locus_tag="ACG8K3_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144791.1"
                     /translation="MKKVYQKCLWISYITVGIVGFFFIVAVTLGTIFLWFTNEDLLLK
                     GLMSVISCVLLILLGTEINKLLPSRWQEKKKEMREEIEIKSTDWIKHTWLPLKRNSKR
                     KEFWILQLWPLGKLCSYLARFILDLIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTL
                     IFVMLLSIFTQIVMQFTRQKLTAGKLFKSLFDLIIVNAILATLIKQIEQFGRTTKGVK
                     EFLMTFVHFVNGHMYLFEVGIILFLVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYYVD
                     FGRYDELINTDIVHTSIHEATVKGIRTTLKDGQETKAIDRRKEVYNFYEVEANLRYGI
                     GKLSLNGRNIPQNKPYIGWKSDALEVTKKLPMEEEEIQ"
     gene            complement(4929..5159)
                     /locus_tag="ACG8K3_000890"
     CDS             complement(4929..5159)
                     /locus_tag="ACG8K3_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144792.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            complement(5182..5988)
                     /locus_tag="ACG8K3_000891"
     CDS             complement(5182..5988)
                     /locus_tag="ACG8K3_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144793.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEISTNAFVNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            complement(5999..6643)
                     /locus_tag="ACG8K3_000892"
     CDS             complement(5999..6643)
                     /locus_tag="ACG8K3_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144794.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNLVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(6659..7864)
                     /locus_tag="ACG8K3_000893"
     CDS             complement(6659..7864)
                     /locus_tag="ACG8K3_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9144795.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKNSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            complement(7864..9888)
                     /locus_tag="ACG8K3_000894"
     CDS             complement(7864..9888)
                     /locus_tag="ACG8K3_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFP9144796.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(10051..10644)
                     /locus_tag="ACG8K3_000895"
     CDS             complement(10051..10644)
                     /locus_tag="ACG8K3_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144797.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(10645..10977)
                     /locus_tag="ACG8K3_000896"
     CDS             complement(10645..10977)
                     /locus_tag="ACG8K3_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144798.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(11068..11541)
                     /locus_tag="ACG8K3_000897"
     CDS             complement(11068..11541)
                     /locus_tag="ACG8K3_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144799.1"
                     /translation="MEDILIATFITALGAFLTIFVATIKGFKDTESLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTELFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENLNRLNDEMQRVHLENEELRAQNYELIQMKRNLEQKIFELKEEN"
     gene            complement(11602..13962)
                     /locus_tag="ACG8K3_000898"
     CDS             complement(11602..13962)
                     /locus_tag="ACG8K3_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9144800.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKIIGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQKVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLKDIKP"
     gene            complement(13959..16568)
                     /locus_tag="ACG8K3_000899"
     CDS             complement(13959..16568)
                     /locus_tag="ACG8K3_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFP9144801.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNTAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(16568..18631)
                     /locus_tag="ACG8K3_000900"
     CDS             complement(16568..18631)
                     /locus_tag="ACG8K3_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144802.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGQLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            complement(18701..19003)
                     /locus_tag="ACG8K3_000901"
     CDS             complement(18701..19003)
                     /locus_tag="ACG8K3_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144803.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            complement(19021..19263)
                     /locus_tag="ACG8K3_000902"
     CDS             complement(19021..19263)
                     /locus_tag="ACG8K3_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFP9144804.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(19260..19724)
                     /locus_tag="ACG8K3_000903"
     CDS             complement(19260..19724)
                     /locus_tag="ACG8K3_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144805.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            complement(19751..21841)
                     /locus_tag="ACG8K3_000904"
     CDS             complement(19751..21841)
                     /locus_tag="ACG8K3_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFP9144806.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            complement(21838..22098)
                     /locus_tag="ACG8K3_000905"
     CDS             complement(21838..22098)
                     /locus_tag="ACG8K3_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144807.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(22111..22872)
                     /locus_tag="ACG8K3_000906"
     CDS             complement(22111..22872)
                     /locus_tag="ACG8K3_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9144808.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(22888..23640)
                     /locus_tag="ACG8K3_000907"
     CDS             complement(22888..23640)
                     /locus_tag="ACG8K3_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9144809.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIVAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(23655..25631)
                     /locus_tag="ACG8K3_000908"
     CDS             complement(23655..25631)
                     /locus_tag="ACG8K3_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP9144810.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGAGGITVNGVKSETSPYSVFKWGDIA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(25684..26354)
                     /locus_tag="ACG8K3_000909"
                     /pseudo
     CDS             complement(25684..26354)
                     /locus_tag="ACG8K3_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(26436..27452)
                     /locus_tag="ACG8K3_000910"
     CDS             complement(26436..27452)
                     /locus_tag="ACG8K3_000910"
                     /inference="COORDINATES: protein motif:HMM:NF037933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9144811.1"
                     /translation="MTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRVDT
                     FYGDLIVPTIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIW
                     NPSNKLSDIWINTNTIGSSLMTLALLITGFASSFVKWYMRLLSLPALILAGATIWVCQ
                     SKAALYALVIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGR
                     EEIWRKFYETLGEKTGQIFVGMKPFFFHIGNQILGNHNTYHALVNHYGLIGFALSIIP
                     LLSFLWLLVRKCELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQY
                     KTTR"
     gene            complement(27604..28293)
                     /locus_tag="ACG8K3_000911"
     CDS             complement(27604..28293)
                     /locus_tag="ACG8K3_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HFP9144812.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(28420..28658)
                     /locus_tag="ACG8K3_000912"
                     /pseudo
     CDS             complement(28420..28658)
                     /locus_tag="ACG8K3_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28724..28963)
                     /locus_tag="ACG8K3_000913"
     CDS             complement(28724..28963)
                     /locus_tag="ACG8K3_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144813.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            complement(28989..29111)
                     /locus_tag="ACG8K3_000914"
     CDS             complement(28989..29111)
                     /locus_tag="ACG8K3_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144814.1"
                     /translation="MKFFEVKSPCYYALIAENNEEECAEITTNNKKLVLSLDIE"
     gene            complement(29130..29471)
                     /locus_tag="ACG8K3_000915"
                     /pseudo
     CDS             complement(29130..29471)
                     /locus_tag="ACG8K3_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            complement(30090..30422)
                     /gene="traE"
                     /locus_tag="ACG8K3_000916"
     CDS             complement(30090..30422)
                     /gene="traE"
                     /locus_tag="ACG8K3_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9144815.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(31247..32287)
                     /locus_tag="ACG8K3_000917"
     CDS             complement(31247..32287)
                     /locus_tag="ACG8K3_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9144816.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         9308 a         6565 c         5012 g        12186 t
ORIGIN      
        1 ggataatctt ctgccgcatc gacgaacgtc ttcgtagtca gatttaacgt taaaatatcg
       61 ccactttgat aagcttttgc agctttgtct gtgactacaa aagctctgcc tgtttgaatt
      121 tctgtcacaa aaacgagatt gtctctgctt ttttcaacaa caactccaat agttgaataa
      181 gccatttctg cttcctccct tctctttctt ttgatataat gtagtcggaa atcgcaccac
      241 tcaatgaatt ggcggaaaac aattcagtga aaaaaaagag tcctggacaa tcacgttcag
      301 gactctttta cttcttttat tcacttaata actaacactc tgtgagtgaa atagactctt
      361 ttatgctttt acatggaaag ttattgattc cctcttcatt catttcccaa gtagtataat
      421 aaggtgttaa cttggcctca tattcttgaa tgataaaata tttttcaccg ttcacatgat
      481 tacaacaata cacaccagta gctgggtact caacgactaa aaattaattt ggatcaatct
      541 gttcgcttct caacatttct cttatctgaa acaaatgctc tttttccaaa caatcgggaa
      601 gttgttcgat tcgttctttt tttagtgatc cttctatgac tttccattga ttcaaatatc
      661 gtttcatttc tcttcctcct actgtctttc ttgttccatt tgaattcttc gttgttgctc
      721 ttcgtatgct ttacttgcgt ggtatttttc ttcatgggca ttaatcgttc gatttaattg
      781 acgcaaactc cgctgcgtga caactctttg ttggaattgt ttcctgcgct tcatgaattc
      841 ttcgtaacta tatttgtatt cattcgttgg caattgttta agcgttcgaa aggatcgttc
      901 attgtaaata gattgcatct gatcaacact ttctattaat ggttttattt gactatcctg
      961 tctcttcagc tctgttagga tggcatttcc tgtgcgcttt tttaaatcaa agagagcatt
     1021 tttctctgca tcctttgatt ctctcttgct ttccccataa agattttttg cttcttcttt
     1081 caatgctttt gaaatctgta tatattcttg gtattcaggt tcttttgtta acacatattg
     1141 ggttaactcg tcgagtttct ctcttgtgta tggtgaaagt ttggttcgat tttcatcccc
     1201 ataattccat tccgaacgtt tttcaggtaa gtttctatat acttcattta ataagttcat
     1261 tgcgtgtcga tcataacgta ctggctcttt taccactttt gttaagctgt tacgcaaatc
     1321 actttttcgt gtcaataaat ttcgtttctc cataccaatc attcgatcct tttcataaga
     1381 gaaacgttct aactcatgca cataattggt tttcattgcg tctaatgttg cttgccttct
     1441 ctttcctttc ggtacaacat aactcacacc attttcaacc tcagtgtaag gtttacgcgt
     1501 attttctttt tcaacaactg caaaatgaat atggatatgt ttagtgttac ggtgaatcgt
     1561 tgccatccag tagcctgttt cgttcagttg ctctttctcg aataatgtct ccatcatttt
     1621 tcttccagct gcttttaacc cttcttcatc taaagtatct gttttgggat cgtacaatcc
     1681 tattttttca agatacgaat tatcatgaga gacaacatct tgataaatga ttgagccttt
     1741 ttgttgtcct ctttggaaga tttgcttaat ctccttcaga tcacttcctt tcacttcgtc
     1801 tgaagccata gaaaaaactc caggaaggtt tcctgaagct tttttgtgtg gctctgtttg
     1861 atattttttt attgcttggt tcaagcgttg gtattctttt tgttcccttt gcgtcagttc
     1921 tttcttttgt tctaacgcat agtgacgggt catataagaa agatatttgg taaaatcttg
     1981 ttcctctaac ttgtcaaagt tgattccatt tcccatcaaa gattgagctt cattcgacgt
     2041 ttcgctgaat tcttgatccg tcttactctc cgaaaaagtt tctccttcag gaatataaaa
     2101 atcctctaaa gcattcttga ttcgttgcag ttcctgttct tcttcaggcg tacgtcgttc
     2161 catttcttcc agcgcttttt ctcttgtcat ataatcaatg tatgttgaaa aagatttttt
     2221 attgggcaag gtaaattgac aagtgagaat gactgcaggc gatttattca tcatgttccc
     2281 cttctacttt ttatcttttt aaaaactttg ttggatcaaa cgggtctact ctggcatgaa
     2341 cagattcatt tattgatttc ttgattttag gagaatactt cttcgtttga ttcgctttta
     2401 ttttcccttc cacacgctct tttgcttctt ggtaaacagt tgaatttgta ccaattaaaa
     2461 tccctgtatc tttcaaatga agatccgcaa actctgaaac catctcggtt aaaatagaaa
     2521 tttcttttcc cattgcattc aatttaattt gaattggatt tattttttct tcgatttttg
     2581 gatcatacat catacacaaa tgtctgactg cttcactata attagatata tcttcatgag
     2641 tagaaattat ttcatcaagt acttcgatat gcatatcgta aagatataca tgttttcttg
     2701 ttgcttttgc caaaattgat ttcctccttt tataaattca caatactatt tttattattc
     2761 aattaacaaa ccaattaatt gattatttgt tgcaatatat tcttctaaag tttttgtatt
     2821 ataagtttga atatccacaa atttttctaa caaccgatca aattggtctt ttttataatt
     2881 cagaagagcg tgatcaaaat cattttcgat caaaactttt aggtattgat ttcgactcca
     2941 ttttttgcca gtcttgaccg taagttcttc acaacgtttg tcaattttat taatgtactt
     3001 ttcttcaata tttctaatta ataagtccat attttcctcc aaatttatta tgtgtaggga
     3061 tatgtgtagc tacacatatc cctacacatt ttttgatttt gctacacaca aacgcttatt
     3121 ttactgccca cattcgcttt catcctaccc acaaatctac acacaaatcc aaaattttga
     3181 tatcatttat ctttcttaga aacgtggtag gcacagccta cctagcacct ttcgtaagaa
     3241 agtgatatca cgtttcctta tgctctttaa aaattaaggt tatttgtgta tttagacaat
     3301 aaaaaatcct tctaactttg tcgattagaa ggaaatattt tgtcatatga aaaatgaatt
     3361 taagaaaaag tgtatgctat aattgatacg ctgtcttcct gtttggttga aatcttcagc
     3421 cagaaaggag gagtgttaaa tgttttatgt gcttgatgtt tgcgttagaa tcgtggttaa
     3481 tgttttctcc catctcatta acaaatggtt agattctaaa catgataaag acagctagcc
     3541 tgttcattat ttggttaata gtgaataaag aaaagagcct tacgcagctg caactgctag
     3601 ggctcttttt gagtattaaa tgttttatgt gtttgcatta tcagtatagc atacactttt
     3661 ttcacagaca actttctaca ttttttcttc ttttttcaat caaaataaac taatgaataa
     3721 tataaactct tattgagaac tgttggaaat gattactgat atcttcaaac aaatgattca
     3781 aattgactag actcattgaa tctcctcttc ttccatagga agctttttag ttacttctaa
     3841 agcatcactt ttccagccaa tgtatggttt gttttgtgga atatttcttc catttaacga
     3901 caattttcca atgccatatc ttagatttgc ttcgacttca taaaaattgt agacctcttt
     3961 tcttcggtcg attgctttcg tctcctggcc atcttttaac gtagttctga tgcccttaac
     4021 ggtagcttca tggatactcg tgtgaacaat atctgtattg atcaattcgt catatcttcc
     4081 aaaatcaaca tagtactcat aaccaagaag tgtcggaatc aagtcaatct caatttcttt
     4141 ttcaattgaa cgtttaataa gtttcgtgga tacatacccg actaaaaaga gaataatacc
     4201 cacttcaaac aaatacatgt gcccatttac aaagtgaaca aacgtcatta aaaattcctt
     4261 cactcctttt gttgttcttc caaattgttc gatttgtttt attaaagttg ctagaattgc
     4321 atttacaata attaaatcga atagactttt gaaaagtttt cctgctgtta atttttgcct
     4381 ggtaaattgc atgactattt gagtaaaaat agacaatagc atgacaaaaa tcaacgtcat
     4441 tagcactgta aaaatgtctg atgctgcaat cctattggga tcatttaata agtaataagc
     4501 cgttccaaat actacaatcg agtaaatgat taaatcaagt ataaaacgag ccaaatagct
     4561 acaaagttta cctaatggcc ataattgtag aatccaaaat tctttacgtt tgctatttct
     4621 tttcaaagga agccaagtgt gtttgatcca gtcggtcgat tttatttcaa tttcttctct
     4681 catttctttc ttcttttctt gccaacgaga aggcagaagt ttgttgattt ctgtccccaa
     4741 caaaatcaat aggacgcatg agattacact catgagtccc ttcaataata aatcttcatt
     4801 agtaaaccat agaaaaatcg ttcccaaggt cacggctaca ataaaaaaga agccaacgat
     4861 acctacggtg atatagctga tccataaaca tttttgatac acttttttca cataaaatcc
     4921 tcctgttttt attttctttc agccgtaaaa tattttttag cagctaaata caaagcttca
     4981 aatgatctga attttaaggt atctaaactc tttctaccat tcttcaactc agatattgtt
     5041 gtgttagata aattcacttc ttgacatatt tctttagctt ttacaacttt gagataggct
     5101 ttaatcaata ctttctgctc agcgaaagta tctttgccga ccaagttcgc tcgcttcatt
     5161 tcttttactc cacctattct tctaaatatt ttcttttgtc atacgatcaa ttttttcttg
     5221 aagtttttga atgatctctt cttgatcttt catttgcgtt ttcgatgttt caatttcttg
     5281 ctggtaacta ttaatcttat tttcagtatt ttcctgatcg tctgcaactt gataaggcaa
     5341 ttgatctttt agtttcttca ctaattcttc attcaaatca atggttgctt ggaaggattc
     5401 aatctgtttt tgtgctgctt tttgttcttt tttgtatcct ttcactttgt aattgatcgc
     5461 atgttgttca taatcttcat ctttcacttg atctttaacc ttatcttcgt gaacgtaata
     5521 aatcagatct tgcggttctt gcatatccac ttttggatca attttttcag gaataatggt
     5581 taaacgcatg gcaatatatt cttctggtac atgagatagc ttcactgcaa aataatcatc
     5641 agacactttg atcactttgg cttttattgc ttgataatcc cctttagctg tctttacttc
     5701 tacgcgataa ttaagattag ccagttcttt taataagctt tgatcttcat cgtcgattgc
     5761 aaattcaaaa actaattgct ggttcgctcc tttttctttt gaaaccatcg tcagttcctt
     5821 cgtgtttaca aatgcgttcg tgcttatctc atctggtatg tctaccttct tatgcgtata
     5881 aattggacta gcaagaatca aaaagccgag aatcataaag atccccagcc atctaagata
     5941 actttttcgt tttttgaaac gttgtcttgc tccttttttc ttactcattt tcttcctcct
     6001 aacgagtgaa ataactaaag tctgtgactt tccattgacc atctacttgt ttttcagtga
     6061 cagttaatcc accttttatt ttttgcggtt tgacatcttt gaattgagga ttgccgattg
     6121 tataatcaac aaatacgaca agattttgtt cctcttttac actgcttgtt tgagcgtaag
     6181 tttgcacgtc acctaatgta aaggagtaac ttccgtaatc atcatgttgg tgatcatcgt
     6241 ctgcaggact taatttgtcg atcacttctt gggttgcgac atcttttagt ttttccgtta
     6301 attcatttct gcttaatgat ttatcaatgg ccaattgacc gaactcttct gccgtctttc
     6361 tttgtgcttc agttacttcc tcttttgctt ttgtgccatc ttgttgcttc agctgatcga
     6421 tttcttggtt tttctcattg atggtttgat ttaacttgtc gatcttcgcc tgtgcctcag
     6481 aggaattttt tttgagcttc tcagacgcgt ttttagccac ttcttcaatt ttaaaggtat
     6541 ttgtctcttg ttcttctttc agctgccaaa acaacgcgat attgccgatt aaactaacgc
     6601 taattgcagc tgttaagacg atttttaaag ttttattttc catatttttt ctcctgatct
     6661 attttttgct gctattcaaa aatggtaatg gatcaatcca tgtaccatcg tctgtgaatg
     6721 cgtgtccttg tgcttgacgc caatccattc gagtgaaacc taaatgcaag tgtgctgtat
     6781 ctcgtatccc gattacttga ccaactttca cttgatcgcc aaccttcaca cgagaatttc
     6841 ctgtactatt cgcaaattct tgatatacca tattcaaacc gtcgtaatta atcacgatca
     6901 cacatgcacc taaacctgaa ataccaggat tgccaacgta cacgactttt cctccatgca
     6961 ccgcgtgaat ttcacttcct ggatgatcga ctgaaccaaa gtctaatcca tcatggaagc
     7021 cattcggacg gaattcaccg cctggatttg ttccaaataa ttgaccacca ctgaaggaat
     7081 ttttatctaa actgctgcca gggaatggat tcgtccattc accagtgatc gtgccgttgt
     7141 tattgccacc ttcaccccaa tgtttgatgg cgaattcatc atattcggtt aatttattag
     7201 aagtgataat ggacatacat cctgtataat acggactatc ttccgtaatt cctgcagcgg
     7261 cataaccgcc tcgtcctaag atcaataaac ttgcgccagg gcttttattt cctacacatg
     7321 cattaaaatt ggattgtgtg gctaccataa agccgtaata tgagaaggca gcttttgagt
     7381 tgggaaactt catgtaccag ccaccttcaa tgccaccaat tcctcgtgca gttcctttag
     7441 gaaacagaca accatcaaac caagtaatcc caaaatagtt gagatcattt cttgcggacg
     7501 ccgacgtacc aaacgctgat tcataaccgt attgcgaaag gatcacactt ggtaataatt
     7561 tatatttcac tgctgcacct acagcatatt gcgcaatctc atcagaaaaa gggcctcttc
     7621 ctttgatttc tttataaatc ggtaagcctt ccgaatattc tcctgtaaaa gcttcgccac
     7681 cactacttga atctgaatca gaattatcaa tcgaacccgc cgcaagtatt agaaaaaggg
     7741 tcacgaacac agtaatcaaa atagtaatca aacctgcaga agtaccaaac aaccacaaaa
     7801 ataagcgtcg ctttgcacga cgcttaacat actgcttgat taaactttca ttttggtttt
     7861 ccattatttt cctccatcaa acaattttaa ttgacgtttg ttcggactaa aatgaacacg
     7921 aatggtttct tttgagccaa aattgatcac agcgtctcct tgattttgtt gaggaatgga
     7981 ttctaattct gagtttgtca ttgatttacc aactaatcct tctatatgac gtaaaataga
     8041 ttcatccata cgcataaaga tcttcgtaga agaaaattca aagactttct tcagtttcga
     8101 gaagttatca gaagacatat tttctggcac catttcttgt ggggattgtg tagctaaaac
     8161 agtcattgct tctactttac gcatttctcg ttgaaagttt gtgacataat ttaccgtttc
     8221 aataaaatct ggattaatcg cattatggca ctcatcaatc atcgccaaga agcatttgaa
     8281 gtcttgttct tccatttgtt tttctttaat taaacgtcgt tgtcttcttc cttgaatcaa
     8341 tgcctgattc caaatcaacg tcatagccat gtacatttga cattgttgta ccccacgatc
     8401 taaacctgta atagagtcaa tgtcaaaaaa gaccaactgt tgttggtcaa ggtgtcgaat
     8461 cgtactatga ccatcaaaaa tcatgccata cgtctcacat aaggattcta cgatggattc
     8521 catttcttct tttcgattct ttttctcttg ggaagcacct ggtaattcca gttgtcctaa
     8581 aaagtctaaa aattctttga acgtcggata agcttctgga tctttccctg tgacttgcgt
     8641 ctccgtattt gaacgcgagt ttgaggaagt gaaaccgcgt ggtactgttc catactcttc
     8701 ataaaaacga tttaaatagg tcttaaattc tgctttcatc gttcctttta gctgaggact
     8761 cactaactgt acctgataga ccactttgtc taagtgcgtt tgataggacg ctgcttcatt
     8821 gatcttcaaa tctttgactg atccatcgtc atataaactg gaagcttcat catacgtacg
     8881 tgtggcaaat atttctaaag gattgatcat cccgtcagat ccgtctaaac gcaccataat
     8941 gccgttatat tcctttaaaa agggaataaa gtcttttccc ttttcaaagc cccaaataaa
     9001 gtctcctgca gctaaatggg cttccgcgat cattttaaga acggtcgatt taccagctcc
     9061 catcattcct agaagcaagg tattggtaga caaccgcaca ctatttttat acgtggcatt
     9121 ccaaacaaac gggccgcctg tcatggtttc cccatattgt gttccacctg gatcttttag
     9181 cgtttggtgg tggaacggga aacctccgcc aatgtttagc gatggtagtg attgcccttt
     9241 gatatttcca aaccattcag cttgttcatc gaacatggtg aacatcgatt gccagtctct
     9301 tttcgttttg tgcagatagg tcactgcacg ataatccatc cctttgagat tttcgatcag
     9361 ttcctgtgct cgttcatcta cttcttgaat cgtgtttccg taaacatgta ctctcacttt
     9421 gacttgttta gaaatttccc cattagaagt caattttcgg gcatattccg ctagttctag
     9481 gtattcttcc gcgcttttat ttcggtcagt cgcttttcgt tcattatctg accgatcatt
     9541 taattcatct aaggcacgat tgatttgatc aatcactttt tgcttttcat ctgtgctaac
     9601 atctagtgtt gccactgtat gaggattgtt catgagattg gccaaccaaa aaagcgtagg
     9661 attttttgtg tagtgataaa ctgtcacaca tcgtgaataa ccatttccat aggtcacata
     9721 ctcttcatcg aaatttaatc caccttgtgg catcacttgt tttaaaaagt ctaaatcata
     9781 ccctttggat tttaaaattt ctatttcccg atttcctagt tgctttaatg gatcaagtac
     9841 cttttgatca ttttgtggtg aaaaagctgt tttaattgct tctaacatgt acagtcacct
     9901 cttcttgttt gattctttac ttggttaaaa gattttcctt ttcccaactt acccaggctt
     9961 aaagtgtgag gaaattttct agtggtctct tctttcggaa catctttttc taaccatatc
    10021 aacggatatt tcattctctc ttccccattc ctactcatca ttcatattat tcattcgata
    10081 aagaatcttg atcaattctt ttttgtagag tggttttaac cctaaaatgc cactagatcg
    10141 cattaactga cggtttctgg tttgcaaatc cttgattgtc tttccttcaa tgacgaaaaa
    10201 gaagcttaaa tcagggcgtt cctgttctgc tcgtttcaat tcctggattt cctctactaa
    10261 tttcttttca tattttctta atcgctgtag atttcttcct aaggactgtt ccatacgatt
    10321 tttatctaat aactgccgct tttgcacgat ttgctccgtt agatccgttt ctgtggtcaa
    10381 actaactaac ttaatcgttg gcacatacgt acgcatgaca gtggtcaact gattcattcg
    10441 ttccagtttc tcttgttcat ttaatccttc cacatctgta gaaactacac gataataacg
    10501 ttgaaaatga tcttctttag taattaagac tccctcttct gtacaggatt tataaggaaa
    10561 taagtccaag atagagcgct cttctggttt tccaaactgc aaaatggatt ttctgtcaac
    10621 tgaattttga cctttgactc gcatttattc atcctcctta tacgtaccat gataatttcg
    10681 gttaggatct aacgcatgat aatggttctt gtccgtaaaa aaaatagact cgataatcgc
    10741 aaaaatctgg cgttgacctg ggttattata gggtcgccaa acaatcacta aagctatcgc
    10801 aatccataag ccataattca agagtgcaaa aaagagtggt aagtggacaa gattattgac
    10861 tattttcgcc caaaaaaagc aaccaccaat cgttaataga tctaaaaaga aaaaggcgaa
    10921 aaacttccat tccccactaa atcttggtgg atttttcata tgaatgtatt tatccataat
    10981 gtaaccatac tccttatgtt ctttattttt agaaacaaaa aacactgtga tttctcacag
    11041 cgtttttcta aatattataa ctctaattca attttcttct tttaattcaa aaattttttg
    11101 ttctaggttt cttttcattt gaatcaactc ataattttgt gcgcgtaatt cttcgttttc
    11161 caaatgaact cgttgcattt catcgtttaa tcgatttaaa ttctccaatg attcttgtat
    11221 cgtttcttgc ctaaaattaa gtgagccaag ttgacgatct ttttgttgtc tttgctcttt
    11281 caattctgac gatatttgct caacagaatg aaatagctcc gttaatttta ctgtgaatgt
    11341 gggcatagca tctttcaaat aagtgaatcc ttctgaaagg tgtacaataa tgctttcggt
    11401 atgtgttttt tgaccatcta aaatagactg atgatctttt tgtaaactct ccgtatcttt
    11461 aaatccttta atagtcgcca caaatatcgt taaaaatgct cccagtgcag ttataaacgt
    11521 agctattaaa atatcctcca caattttctc ctttccattt taaacttgtt ttataaagaa
    11581 aaaggccctt aaataggcct tttatggttt gatatccttc aatagttgtt ctttcaatgc
    11641 attgttcctg tttttggtat ctcttgtcac atttattttt gtcggtgcag taaatgccac
    11701 atttgctggt ttgtaacctg tgtgaacttt ttgaataggt tctggtgcag cttctcttgt
    11761 cacaacattt tgaacggacg cattccctgt ctccccactt tcagataaat tgtaagaaac
    11821 agttgtcaga tttccatttt caggcaaacg acttggtttc atttgaacat tacctgcagt
    11881 tgattggtga acaaagcttt gatcattctc cttgaattga ttcaaacgac tatctttcat
    11941 tggtaaattt tgtgccttag gtaaaccatt tttcgttgat ttttcagttc caacttcgac
    12001 cccattatca tttggcagat cgatggaatc atcatccgtt tgtacttctt gatttcgttt
    12061 tggtaaatct gaaatgcgct tttcattttc agaattaatg ttagatttat aacgatattg
    12121 ttctggatct gtttttggta attgaccatt ttgtccttct tctgaatttt cgttagaatg
    12181 caactcatct tggtgatctt tggctgaagg aatcgctgca ccttcttgat tttctttttc
    12241 ggttgttgta ttagctttta ctgctgcatt gacttgtcct tcaccatacg catcttttag
    12301 tccttctttg acttctgaag ctttttcttt ttgtttttct agtgtttcgg atggattctt
    12361 cacagcttca gccaattctt tagctctcgt gccgtctaat ttctcttcta aggtgtcttt
    12421 ggctttttct gtaagatctt cagctgttcc ttttaaccca cgttcattga cgtaaccaag
    12481 accttctgcg gctttagaaa caccattttt cgctttcccg aacatttcgg aaactgtttt
    12541 cttactatta ggtacagttg tagctttatc gtctgattgt tcttccccgt tatcaggggt
    12601 aacatctttt cgattaccaa atccattttt acgaatatca tttaaattgc ttaaaccact
    12661 cttaccagca cttgcaaggt ttttagcccc gctacccaca tttttcgcaa catttcctaa
    12721 ggcaaacatg ctaagaagag aattgctgcc gtttttcaga ctcgtatcaa cgccgaaaat
    12781 tttcgtaact gattgagagc ctttgaatag cgcaatggtt aatacaatta aagcgacaac
    12841 atacaagaat ccaatgatct ttcctgccgc atgcaaatca ccgatacctg aaagcattaa
    12901 tgtatagatt cgtaattcta aaatcgtaaa tgccaataac aagaaggatt gagaaatatc
    12961 ttctatagca cttttcatcc gttgtcctgt ctcaaagtcc gttgacgcgg caatcactaa
    13021 atagaatttt ttaaatacca gctcaataat gcaagtaaca atcgtaaaca agataaataa
    13081 ataggctacc gacaaagaag attcaccagc taaaataata cctggactac taatgtatcg
    13141 gcgatagcct gaagtataca catattttgc taaaccttca tcactaattt gaacagggat
    13201 ttctttccca ttatcgtcta actctagtcg atactgtaat gcctttaacc gatctttttg
    13261 tactgtacta atatcacttg attcagacat tttcttagct tgatcaggtg taataatccc
    13321 attgatattc gctgttttaa aatttttcgc cgtgagagca tttcttttgt ctgttggtat
    13381 gtgttctact ttgttcggat cattttctag taacgtactc acatcaatca cattattttg
    13441 aataatttga aaagcaggtg aaccagtttt atctgcttta tttgctgcag tcacgtctcc
    13501 gtaaaagttt ttactaatat ttagcccttc atcgattaaa gaactaccac caaaaatcaa
    13561 aatcatggcc ataaatagat taaccaagat atttttgatt tttggtggat gtttactcat
    13621 aaaaaatttt aagcctaaat aaattaatgt gacgacacaa aaaagagcca ataccttact
    13681 aatcaaactt tgaaacagtt cattaacacc agcggcgttc aataagtctt ttaaatcaaa
    13741 gctttgataa attgtttttt ctagtaaagt attgagatta tataaccctt taatcaaacc
    13801 ccataaaaaa gtattacaaa tccaactgaa tacatcttga tactgcagat aattaccata
    13861 atcttgtaag actttcgtta cggttttggg atctgcatcc ccatccccaa ataagcctga
    13921 ccaccagtcg gcaatgccgg aaaatattcc tccaatgatc attctttcac cgccttttca
    13981 gcttctagca aaagtcgttt ccttataacc tctgtaattt cttcattttg acgcaatttt
    14041 tcaaggtatt cttgcaaacg atctatttgc ttctcttcat aaaattgaat aagtaacttt
    14101 tgtgccgttg agttgtctaa agattgaacg attttaataa aaatgttttc tttagcttcc
    14161 tcttctattt gatcgtgcat attgtgataa agtcgacgtg catagatatc aggtagaaaa
    14221 taatcgtaat caatttgtaa agccttaaga tccaagtccg catgtgtaca atgtatgtct
    14281 atatcattga tatcccacga tggattgaaa tcttcaatat attgataagc ataaggaaga
    14341 ttcgtttccc catgattaaa aataggatac ggccgtattt tatgaccttt caaatcttgt
    14401 cgttttaaag gagcgagtac aatcgtttca ccttctaaaa aagtagaaat tctttcagga
    14461 gttaagatgc gatctgaatc aacattgcga ttgatatgag tattcatacc taatgaattg
    14521 atggtactgt tctcttgttc aaccgtctta ttccccactt ttttggaaaa ttcttctatg
    14581 gtattatcgt ctgtcgacat aatataaata tgtgtctgac aattttcttt gataatcgct
    14641 gcgttttgtt ccccatactt cgcataaagt tgctgatagg attggataaa caaatcccac
    14701 aacatgtttc taccagctgt gactgtcatg attccttcca tattatccat tggtggcata
    14761 ttcccaaatt catctaaaat aaaatgaatc ctacggtgac atttatctcc tttagtctta
    14821 acacattgcg tagataattc tttatacaac tgactaagaa aaatactaac aatgatatgg
    14881 ttagatggat cataatcagg tgtgagcata aaaatagcca ttggtttatc ggtatagtgc
    14941 atccgaatag actctactaa taagttactt ttatcctcat ctaatgttag aacggcttct
    15001 ttttctaatt ctggcaaatg ggtttctcct cgtttttttc gataaacgat atttccctct
    15061 tcgtctttca atactctttg agaaaactca taccaagcaa catattcttt tcctcgatga
    15121 tcgtatttta agcaaacatg atctccctgt tctaaatgac aatcaaagtt aatttcacaa
    15181 aaacctctat agcctacttt gacacgttcc tcatgaaatt tttttccttg atgataaaac
    15241 tctacaacaa ttcgctggtt taacagactc tcgtgtagtt tgagatccaa atacttcgga
    15301 aaaccaatgg acttataatc aatggttgat tgagaagtca ttttcgcaat tttcgtcagt
    15361 gtaaatggat ttagtcctgt gatcgctgtc gatagaatgt tgcctttcgc tttatcagaa
    15421 ccaatttttg ttgccccata ttgttttttt gctgtgtttc caggtggtaa ctgattcata
    15481 aattcatcta aagcattgag ctgcttgaat tcattatcag gatcttggta attaaaacca
    15541 ccaatctcag aaagaagttc gacgagatta ttcggtgtta atttggagta atcccctgtc
    15601 tgatccgctt gttccaataa cgcaaaaata agcgcattga cagctttttg cgcattttcg
    15661 ttaaagtatt tattgtctga agcgttggca tcataataaa tcgtattcgt gaaagtattg
    15721 gttaactgtg tcgctgtgtc tatgtctcct cgcttccagt atttcacgat gagtgccaat
    15781 ggattatcac tcattgattg cagtggatcg gtgatattaa atgcatacac atcataccct
    15841 ctagcttcta gttcatcttt actagcaaca tataattctg tttttggatc attaacaacc
    15901 attgaacttt gtctttctgc acgagacaaa ttgtcaatgg ttgctaaaac ttctatctgt
    15961 cctttccctg aacgggaagt accaacaata caagtattta ctgtatccgt ctgaatatac
    16021 cagtactttt tataatgagt aattggtatt ccgccataac caggaaaacc tccctttgat
    16081 tctggtattt gcggataaat ttgttggatt tcctttaacg tttttaatcg gttatctcct
    16141 ttttgcccat ctgctagagg acgaaaccac atgtccattt tcaatcccat aagtacagct
    16201 ggaacaacac aaatcgtaga aacaactaaa cgtgtgccta aacttgttgt tggggtcaaa
    16261 ggattccaaa aaccagttga atcaaaatgg aataactgcg gcatttcttg taactgaaaa
    16321 atattcactc cttgtaaact tcctggaata atctcaaatc gatgaaatgc ttctggtatt
    16381 cttgtaacaa catagtttaa aggaagataa gctaagccac tataaaaaat cttttgagaa
    16441 atacgtttgt catgtttttc tggtagctct tctcctgaag gagtatagcg atagaaatga
    16501 gaaagatacc tattcttttc taaattgaat cgtttgtctt ttcgtctcgt tgtttctcta
    16561 acacgcatta tttcacctca tttgattctg aactttcgga agtgtttgat tgtacagttg
    16621 aatcgcttga tccttcttta gccttactag gatcactctc tttgctattt tccatacttg
    16681 attctgttgt tgactgattg gtacgattca ataattcttc taattgttgt tcatactctt
    16741 ttcttgcttg ttcttgggct tcttccttga ttgacgtcgt tgtggccaca gaatcagcct
    16801 gtgaagattg attgatatga ttaaaagcaa atactgttcc tccacctaga agcaagctta
    16861 aacttgcaat acatcccacc atccatccgt taaaggaacg ctgtggtgaa ggagttagat
    16921 ttgtttggtt gttcattggg gtttgaacag cagctactga aggcgttggt tgaggttgtg
    16981 ctagataatt taactgagct tgtcgcaatg tatccatttg ttcttgacgt tccttacgag
    17041 cagctgcttc tttttccttt tcttggttta accgttgttt ttctattgcc atttgttgtt
    17101 ccgcaagatc ctgttcacgt ttttttagtt ctaattgttc gcgctctttt tctttttgcc
    17161 gttgtttttc ttcttgttct ttcaattctt gttgttgaat ttcatactct tttttccatt
    17221 ttggaatttg cacttgtaat tcttgctgga tcttttcgtg tttttccata agaacttcag
    17281 tcgttgtttg atatagctta ttcagttgtt catttaattc tttgctaatc tttagcttat
    17341 ctgtacgcaa acctcggtaa aattcttctt ttcgttcttt aatacgttct tttaacaagt
    17401 tttctttttc tttcgataac gcttcttgat tcgtctgagt aaaattattg atctttgcta
    17461 cacacaagga atacgcattg tcgcgtttct tttcgtatcg ctctttaatt ttttgtactt
    17521 cttcttcaat cactcgctgt tcttctttat ttagttcctc ttttgctttc tcccaattag
    17581 acgactgttc cgtcgcatac tcattaattt cttgatctgt ttcttgttga cgttcttcaa
    17641 aggtggctaa taaatcttct tgtgcaagct catgccaatt tgttgattgg acagactcta
    17701 actgttctaa aagtgtgcct ttaccctgtg agattaattc ttctttactc tctaaaaatg
    17761 cttgataggt ttggggtaat tgatatttcg aataaagctc atttaaaaat aatttgtttt
    17821 tttctttcat ccattcatgc tctggcggat attgatccaa aatataatca aatacagttg
    17881 ccttttgtat ctctatttcg tttttttcta cttcttcact gataagtggt tcaatatatt
    17941 cctgagaatc cccttcagtc catgttgact cttcattagc ttcaactgga tcaacttcta
    18001 cttcggccgt ctcttcatcc tccattgttt cttcaaaggg atctacctcc aattgaccca
    18061 tcacatcgtt ctcttcctca aatatcgatt tctgagtcgg aatatcagct tgttcgtcaa
    18121 catagactgt ctctttttct tttgactcct tctttggaaa tattggtcgt tcccatggta
    18181 cttcttcatt tacttcgact tcactccctt gattcacttt tctttggtcg ctctctaact
    18241 cttcttctaa aacttgtaaa agtttttcgt ttcgataaga ctcttccaca ctcatagttt
    18301 catccgtttc tttaaacaca tcaaggcgga ttaaatccat caatgtagga tagaaacctt
    18361 ctgaaatttg ataatccagt tgcccaaaaa cgtattctgt accagctttc tctatttttc
    18421 cttctgtgtc atgtaacgtg agagtcaact taacagaaga tcgactttcg tgttccagta
    18481 ctttccctaa cacatgtagc tgttcttccg tttcaatgac agaatattta attgttttcg
    18541 gtaaaaaaga agtacgcttt cctacttcct tcgttgcttt ttttattaac tctttatctg
    18601 ttttggtatt gcctgcaatc gtaaagtaca tgagtattct cctttcataa aaaaggagta
    18661 aaaagtatcg actacttctt actcctactt ttttactttc ttataatcct tgaggtgttt
    18721 gtccaaacaa tccaaataac gaaaccacaa tagaagctgc tgctaaaata actcctaccc
    18781 ccatcaatac atagcctacg tgcatcttcc cattttcagc tcctcttcgt ccaaatagga
    18841 agaaaagacc cccaataaca atggaagcaa cggccgcagc agcagcaaaa atcatcagat
    18901 tgttttttaa actagttgcg gcagtttgta atgctctaaa aatatcaacc gcagctacta
    18961 ctttcggttg tgttaaaaaa ttcattgtaa ttgattcaaa cataaaattg ttcgctcctt
    19021 ttagtgttta ataatttcgt cgaaaacaag cttttcacgt tcacgttgat agatatatcc
    19081 ttctaagtcc ttgtcttttc cccgtaatag gtcaaattgt gctttttcaa ggacttcttg
    19141 tttccactgt gtttcagata caccgcggcg ttctaagatt ttagacgcgg ctttttcaaa
    19201 tggcgtttta gcttttgcca ctgtgattcc tccttcagga caaataaaaa gactagcaat
    19261 cactcgctag tcttttgtaa cttatattta ttggattgtg atccatcgtg aatcaatttc
    19321 tctttcgttc gcttttcatc cttgctagat ccaagaaaaa ttattttttt tacgccattc
    19381 ttctcatagg gaacaacaat aaataaagaa tcagatttgt ttataaattc cctaacacca
    19441 gctgaatcta ctattttgta tctttctaaa cctttatagt ctttttgcct cttgactgtt
    19501 atttgtacag gatctggatg ccatacactc tcagagtatt cccaggtagt ctcgtctata
    19561 attttcaaat tataaacaag accacctact tcttctttcc acatagttcc tgatttcaat
    19621 acgccattat cttgattagt ttgtttattt ttttcattac tacatccgac taaaataaca
    19681 atcatggcaa tcaaggaagt aactactatt attaactttt tcattttatt cattctttcg
    19741 tttttttatt ttaaatgtat ctcagaaccg cataactaaa agttgctaac acaagtaata
    19801 gtaagctgac cgctgaaatc gttactaaat gtgtagaacc tgttttagga acaatgacct
    19861 gtggcaagtt aggatcaacc ccaattgtca caacatttgt tttattagaa acattatcta
    19921 ctgctactac ggcttggtta tcaatatttt tttctgattg taatggagtt ttagctgtaa
    19981 atatcagctg aatttctcgt ccatctaaaa gattgttact taagaagtca gcggaagctt
    20041 cccacttcag cgttcttgaa ttgctatcat aggttagtgt gccttcagaa gtaatatctt
    20101 ttcctttact cttatctatt acttgcgcag aaccttcttt atatgtcaaa cgagcttcaa
    20161 ttggatcttg aatactaaat tgtttgtagt gatctgacgc agcgtctacg ccttttgtgt
    20221 gccatttttg tgtgactaga tattgtactt cagatcctgc gtttaaagga gctgtcggcg
    20281 tacccttctt accgtcattt cgtccgttta catcttttcc attttgttgt acttctttca
    20341 ctggaatttt agggaaataa acggtaatga tatttgtatc cttttctact ttattaatga
    20401 tcgacgttcc ctgatttttg gctacactat tttgattggc caattcattg acttttacat
    20461 tgatcactaa ttcgtaagtt tcgccgttat acttcattcg attttttaat gtatcagctt
    20521 tcgctgcata ttttacctgg tgttttgttt tgtcataggt cacttcacca gatacatcga
    20581 attttttatt tgtaccactt tcaacgtgtg catcgacgta atttacttct tttggtaaat
    20641 tatcgattaa ctcaaatcga tcgtaaaccg ctagtaaatc cactcctaat cgattcacct
    20701 tctgtttgat tcgatattga atcgtgtctc ctacttgcaa cattttatcg ttcacgttat
    20761 ttccattttt atcaacccct gttttttcag gttgatcagg aacaacacta aagattgtcg
    20821 cagaagataa agtaaaccag gtttgaacat tagaagaacc aatcacaaac gtcggatttg
    20881 cttcagatat tttaaaggac acagtacctt gaccaaactt cggatctcct aatttatcat
    20941 gcaaattatt gaaatcatta tccgcttgtg tcagcttaga gcttcctcct gggtggccac
    21001 ctaccacgtt cagattgttg tagaaagatt taaattctga aagaaccgta tcttttctga
    21061 cataccaatc tgtcgtattc attttgtcat agtgggcata ttctccttta agatccgtgg
    21121 tttttcctcc tgcagggttc aaagagttaa aactgataaa tgtatctcct gaaaattgaa
    21181 cggggctacc gtcctcatat gtggctacta gctgcacatt catttgtgtt gaatggtaaa
    21241 caattcctga gaaaagattg tctgagatcc aaagtactgt tcttttttta gatttactca
    21301 ttgcaccatt cgtattttta tagccatcgt ctcgaaaatg agtttttgta tagttgttat
    21361 ctagtagatt ccacggcaac gtatccgagt ataagtgaat attagataga actagcttga
    21421 tattgacttt tttcccgtta tacgtaccga tttttccata gtcaatattg actttggtgt
    21481 tggttgttac gccttctaaa acaaggtttg tttcaccgta ttctttttgg aaagaaggat
    21541 agcgtatatt tttgtttccc tccactaatc ctacgtagtc gtctgtaaaa ccacctttgt
    21601 tggcatcaac atgatatttg taaacttttg acgctccgtt tacagtaata tttgtttttg
    21661 cattaggaac atcttttgga ataataaaat tattctttac gttctggatt ggatttgacg
    21721 gatcgacaat aactttttca gcaaagacat tggctgtttt taacccaaac aatgtaccta
    21781 acatcatcat tatgggaaaa acatatttta accgtcttgt ccacgagtgt ttaaaattca
    21841 tcccttcatc ccctcttttt tataaatgac tagtgaaatt ccgacaataa cgatcaaatt
    21901 gatcgtagta aataatggat tcctaatgag cttttccata atatctagcg gcatggaagc
    21961 agctggtatg tttaaaatcc tcattaggta tagttggtag cttggcaaaa tagatagttt
    22021 agaaaataat aaaccgcagt acaaaaagac tgcggttaaa caagaaaaat tgaataataa
    22081 taatttgatc agtttcattt tatttcttcc ttaatctcta gaaaacaagt gagtaacaaa
    22141 atcccaacaa ttctcaaaga agtttttctt tacgccagta gaaacgattt tttttacttc
    22201 ttctttaggt tgcgaagctt ctgatttagg atagatccac acatccttta agaattcacc
    22261 gtttttatca gaaacaggtg ttaatagcaa aatatcctcg ctattagaaa tgttttcagg
    22321 acttgattca ttgacaatat aataagcttg tttctgattt gcctgtactt ctatcgagaa
    22381 ttcaaagaca cctgtttgtt gatcaattgt ctttgttttt ccattagaaa ctagcttcaa
    22441 ttgatcactg aatagttttt tcactcgatc ttttaattgg gattcaattt ggtcagtagt
    22501 gagatcctta acatccatct tttgtaaaat gtccgataca tcataaacag aaaacttcgc
    22561 gccatttact ccttcttgtt tttcaagttt ttgcccctgg ttgtctaatt gttcggaaga
    22621 aataattttt tgaacgtgaa tggtctgttt gactatttga tcgtcttcag caattttcgg
    22681 ttcttctgaa gaatcaactg ttgacgaatc gacggtttca tcgctgcttt gaatcgtgct
    22741 gctatctgac gtttggattg tttgttctgt gctatccgtg ctttcttgtc tgtctgcaaa
    22801 cgtcaactgt gtgtaggcag tgaaagaaaa aagagcggct gcacaaacaa atagccattt
    22861 tctatttctc atgttggtta ccctccttta ttttttcttt ttataaacaa atagcgaacc
    22921 aatgatcatg actgaaagaa aaacagtact tcctaaaagc catgtccaag ataatttttg
    22981 tttcttattt tcttcatctt ttaattctga ttttgtaaag gttactcgct tcccatacac
    23041 tagaaaacgg tgtgtgttga tccccttagg cgtacatgtc attagcgtta ctcgatcttc
    23101 attgggattt ggttctaagt aagtgacaaa gtttctttgt agttcgtctt tttgcacttc
    23161 ctcaatccga tcgatttgat aagcgtgctg ctcaccatca actttgatat aaaacttttc
    23221 tccctttttt actgaaggta aatcatcaaa aagattatct ttataaagtc cactatgacc
    23281 tgcgattaaa ggatttttac cgtttcctcc tgtaatatca ccagttcctt ccgtaatacc
    23341 tacaccattt tctaaaattt tgtcggacgt tccgtcataa attggtagtt ttaaagaaat
    23401 acttggaatt gtaagaatac caacaggatc gcctaactca atcttagcaa catccaaaga
    23461 agaatccgtt tctgctgccg ttcctgttcg atcgattgcg acaatctgct gattttgttg
    23521 gtatttcgcc acgctcgctt cagcaggcat caaaagtaag acacctacag ctgaaagtaa
    23581 ccagaaagca atgatccatt tacccatgat tcgctttttc ttaggcttcc tgataatcat
    23641 tggttttgat ccttctaggc tttcttctta aagtaaacaa ctgcagaaag tgcccctgct
    23701 cctagcaaca agcaagagat taatacatac gtttgaaaac cgttgctacc ggtcattggt
    23761 acggaagctt tttgattgtt tttgatcgtg attcggtttt cttctttgta tgaatctttg
    23821 tctactttga aagagatctc tttcgttaat ttttgatagt tttcaggcgc ttttgtttct
    23881 tccagtgaat attcaccttc agttaaacca gtgatttcaa agtgaccttc tttatctgat
    23941 tttaggatcg ctgcatttgc tacatcgtct acccatactg cttctttgac accgttttta
    24001 tccgtatcaa ctaatccacc ataatatttt ccttctgcgt ttttagcagc aaattctgcg
    24061 ccttcaagcg gtgttgcttt gattcctatt ccaaatagtc cgctatcgac ttttaagaat
    24121 tttgcaccat aagtcacaac gtcagctttc gttttttcgt ggtgttcttc ctgattaaat
    24181 ggattgttgt tataaacgaa atccacgcta tttgttaatg gattggcaac agctgcgcta
    24241 tcatttacat acatgttata agtcatttga agatcttgtc cgtgtaaact tgctacagta
    24301 tcacttactt ggccattatt cacgataaaa tcgatcacaa agccattttc tgaattttgg
    24361 attttataat ctgtattttc ttttaatacc acagatccat ctttatttgt tactttgata
    24421 tttgctttag cagcgacata agaaagtcct tgtcctgcta tatcccccca tttaaataca
    24481 ctgtaaggag aagtttcgct tttcactccg tttactgtga taccgcctgc tccgcctgca
    24541 atgtctgttg ggatatgaat cgttccttga taatttactg cgtcaccaat ttggaaagaa
    24601 tgttcttgtc ctgttccatt tgttactgtt ttatcgatca ctggtgcttt atagtttacg
    24661 tatgaagctt ttaagtcact tgtcacgcca tcattcgtaa tttcaaaggt taatttattg
    24721 tgcgcatcgt tgcgagcgtc tgcgcctaat aaatattgat ctgctgttgg ttctttatct
    24781 gatcccacta caacttctga aggaacttcc acaaaatagt attttcctaa cgtcaaatca
    24841 gtagcagtca gctttccttg atcatccgat acaagatctc ctaattttgt tccttttcca
    24901 ggttcgccct tgtataactc aaattttgcc ccttttaaag gtgtttgttt gctttgagct
    24961 gtgccatcgt caccaaattt cgttaattca aaactcaact tagaaacaat atttttagga
    25021 tataagtgaa tgtcttttaa aaaacctgaa cctgtattat cagtcattgg cgcaatcaca
    25081 accattggtt ttgctttttg tgtgactaat ccagcagcgg atttactttc ataaaccaca
    25141 tatacattcc ctttggaatt gacataggct ggttgatcag caaaagtaat ctcaccattt
    25201 tcatctacag ctgaagtaat tttattggtt gcattcttga cgtactcact gttagatccc
    25261 ttgtcttcta catctttaac gatttcgtcc actttcgcgt tggttagatc agaatcttca
    25321 gtcggtaata cttgatccgt aatatttcct aacgtaaact caacatcacc ataatctgcc
    25381 ttgttgtatt tcgttacacc tgtaggatat tgatcgtgcg tataaccatc gtttttgatc
    25441 ccgtcaatat caacattcaa ttgcgttcct tgatcgtaca ttaatttatg aaccgtgata
    25501 ttgaccttct ctggtggcgt tttttgtgtg acatcttctg catatgcttg atacccctga
    25561 aagcagcttg caatgattcc taacattcca acaagcattc cagctttttt tacataccga
    25621 tttattgcca tttttttatc tcctcatttc atatttcaac gactcgcgtt gattaaaacc
    25681 ttgttattgt agctctccct gtaccacgta tctttctttt ccttgtgacg tataaacaca
    25741 agtaaacaac gttaataagg gtttctctcc tttctttggc aaagacaaga aagagttctc
    25801 ttcaggaacg ccatcgatta atttaaagta ctcaggtacg ataaatttac gcgttacctt
    25861 gtaacgatag acgccatcgt tactaactaa gtccatcacc tcccctaatg acaacttttg
    25921 aatatcagaa aataaaacac ctggcatttc catgttatga cctgctagga catagtttcc
    25981 tttgcccatt tcgccatctt cataaaaata agtagccgct ccaagactca aagtgtattg
    26041 atttgtccct ttaaaaattg gcaatgctaa accaagtgca ggaatctgaa tttgtgcaaa
    26101 tacttgttta ttgacgccca tttcgattgc ttctttttgt atggacaaca tttgacccaa
    26161 agacatatca ttattgacgt tcgttgcatc ctccttataa ctctttgcgt gtgcattgat
    26221 ttgatctacg ggtatctttt tttctatatt ttgcttaaac acctctcctt catgtttttg
    26281 gtagctaaca tagccaaaca cactgcaaaa gacgattaaa gcgactccta aaaaaaaaat
    26341 aaccattttt tcatccatct gcctcctttc agataaaaca aaaaacactt tttcacaacg
    26401 aaaaagtgtt ttctcttatt actattctat ttcatttatc tggtggtttt atattggtgt
    26461 gtagccattg ctaacattaa gaatacaatt ggcacccaac tgattgtagt taatgtttct
    26521 tccatgaatg attgacacat gatcgctaaa aaagcccaca agaatgttag ttgtccgttt
    26581 gtcagctcac attttcttac gagcaaccat aaaaaactaa gcaaaggaat aatcgataga
    26641 gcaaaaccaa tcaatccata gtgattgacg agtgcgtgat acgtattatg gttacctaga
    26701 atctgatttc ctatatggaa aaagaaaggt ttcatcccta caaatatttg tcctgttttt
    26761 tcgcctaatg tttcatagaa tttgcgccaa atttcttctc gtcctgtaaa taaatgaact
    26821 ttgtctgaca ttgcagcaaa ataagaaatc ggtaaagcta gaaccgaaac tgctgcatac
    26881 atccaaaagg acaagtgtgt gtttcgaaaa atcttcttag gtaaaatttc taggataacg
    26941 aagataacaa gcgcgtataa tgctgctttc gattggcaaa cccaaatcgt tgccccagca
    27001 agaatcaacg ctggtaatga tagcaatcgc atgtaccatt ttacaaatga actagcaaat
    27061 cctgtgataa gaagggctaa tgtcattaaa gaactaccta tcgtatttgt attgatccaa
    27121 atatctgata atttgttcga tggattccaa atactttgtc cttctggtac tggtatctcg
    27181 gtaaatactc gataaaccgt tacgagtaaa aagacataga atactacaag atacaagcta
    27241 cgatcaaaca cagtaaattc cgtccatttt acaatcaaaa taatggcaat aattgtcgga
    27301 acgatcagat caccatagaa agtatctact cggtagcttc taaaaaaaga aaagaaaagg
    27361 taaaaaagaa tgcctgctgc aatgaagaat aaatcgtttc tctttacctt gtacagattc
    27421 aaaagaaaca aacagcctac gaaaaacgtc actaactgtg cagcattatc ataaataata
    27481 tcgtaggagg atttaacccc accgtccata ttaaatatag aatggatacc aacagtaaca
    27541 gaaaaatggc actcatttta ttgatcaatt tttctacaag ttcttgaatg tgactcactt
    27601 cccttattta ttgatttttt ctacattgac taattcgcac tggatcacat cgcacttttt
    27661 catattattt gcttcaaagt ctaacagtgt gagtagtggt tttttacctg cttttggcaa
    27721 ggataaaaag gaattgtttt ccttagattg aactacctgg ctgtaatcat aaatttttcc
    27781 tcttttggag acttcatact gatacttgta atgagcatcg attagatcaa tttcttctcc
    27841 ttcagataaa tcaatcatat cggataataa aacgcttggt gtatctacgt tataaccagc
    27901 aagaatataa tttccatttc ccatttcagc atctggataa taagtagaag ctactcctaa
    27961 actaagcgta tcgctattcg ctccttgaaa aacagggaga ataaatcctg tagaagaaaa
    28021 aacaagtttt ccagcaattt cacctttgag atccttttct aatgcctgtt tttgattagt
    28081 aataagttgt ctcttagaaa tatccgttaa actggttaaa ttatcttgat aggttttatc
    28141 ctgttcctga atatggattg tttttgttac accatctact ttttcagcga ttttgtcctt
    28201 ttgatgacct tgataagcaa aataactaaa aaggagaaaa acaatgatta cgcccacgta
    28261 aggattaaat aaagttttat tgtttttatt catctatcta ccttctatat ttgaattaat
    28321 ttacctacat ctaaccaaga taaaccgcgt taaattttac tattcttttt attaaataac
    28381 aatgccacat cataattgat cattttcata tcataatcgt caagaaactt ctaataatag
    28441 ttggcaatta gatcttattt tcacataagt tcgtctcaac tgttcaaaat ttttcattga
    28501 tcccaataga caggctttct cctttcctgt tatctgcatg tcactgatat attgcatgac
    28561 agataacctt ttaaggtgtt tttgtttcat cgtttttagt aacttttctt ccatttcttt
    28621 cgacactttt ttctattttc gatttcttta caattcatga gttttcctac cttccttgtt
    28681 ttgataaaaa taaaaaagcc tatgatcaca tacgctctac acttcactct tgggttatct
    28741 ttttctttct tttaactact ggccttgctg gtttcaaatc cttgattcca ttatagattc
    28801 tccaataatc aaactctttt cgtaatcttt ctttttccat atctgttcct ttggataagg
    28861 ctttaaatac atctttaggt gaataactac gagctaacag ctgtacatac cttccttttg
    28921 tcaaagtatc catatccatc gcatctaaaa ctggtcgcgc catctaattt gcctcctaaa
    28981 taaattcttt actcgatgtc taatgataat actagctttt tattattcgt cgtaatttct
    29041 gcacattctt cttcattatt ttctgcgatt aacgcataat agcatggtga ttttacttcg
    29101 aaaaatttca tgatctattc ctccaatttt tattcttctt tatccgctac aaagtcaatg
    29161 atttcaacac tgacaatata atcactcaaa tatgaccagg tatcttctac agacaattcg
    29221 tctcctttgt cgttcgtaag cgtcatttta aaccactcat cgccttcatc atcttcaggt
    29281 agcaagccga atccaattgg attatcagtc aatgtttaat cgtataaatc atcctcgtct
    29341 aatgctaaaa catctaatcc tttacaattt ccgcctcttc taaacgtcac aaatcctttg
    29401 taatcccaac gttggaaaga aaccttaata gtatgtgtgc ccaatcgtat attcggatca
    29461 taatttttca tttgtcattc ctccattacg taaatttcat tttctctatg ttttatatca
    29521 attttttcac tttattcagg acttgttcat attttatcgt ttttcgttta cttaaaaata
    29581 cataaaaaat ttcgactcgt ttatggcgtc cgaaacgaga aaccctagta gacagcggta
    29641 aatgctcgat tgatccaata atcaacgtcc tcgcaagtat ctacatgtac tgcagcacac
    29701 gacacaattt cttgtgcgtc ctccccatta cgagaagacc tactccttat tccatgagtt
    29761 tgcattaaag tctagatccc ttagtagctg ctcatgatta atctagacgc tattctaact
    29821 tgtctaactt ctaaattccc tcttgtttac tctgcgctta ttatttacac aaattcattt
    29881 caccactatc caaacaagcg catttactcg attgaccaat tttttacgtt accaatcgtt
    29941 aaaacctacc ccgtcactcc ccgaagggaa ttgttgatag atcaagtcta ctccttattt
    30001 ttctagtgac tctaagttcc tttagaagtc tcgacaatat tttcaagcaa gcgaaaaacg
    30061 ataaaataca agttatgaga atgatcaatt cagctgatca aacgccccat cccttttgcg
    30121 gtacaacgca agactccatt ttttcttcga ccaatcagta caccgttttg gtcataaaca
    30181 tgtttgattg ttggatatag ctcttttaag gggataggat cttttaaaat tatttgcttt
    30241 tgtttcccaa tgtaataaat catttcctct gagactttgt ctttctggat tatatatttc
    30301 ttacactgca caacggtttt actttggcca gttgatagtt tcttcgtgat tttctctgta
    30361 gcggtacacc gtttaccgta gtgacaaata tctttgagac tcaaaattcc tgtatatgac
    30421 ataaaataaa ccacccaatt tctttaaata tttttaacaa gtttttcttg ttatttttca
    30481 aaaatagaga attgattttc gttcatgaca aacaaaaaag agggaacgca tccaaacatg
    30541 cgttccctct aatttcagcg ttcgtcatac ggcaaagagc cacacggaca tttttataac
    30601 attatgctta ggcatagatt catcgtaata tcacgtattt ttaaccttaa cacagccaaa
    30661 aaaagtggta tcacttttga atatctgtta tactaaatcc gttgcgggta ataccgtaac
    30721 actgaaaggt taacgcatta agggccaaat cttaatgccg tgtatgaacg ctgacgaaga
    30781 ttaccagcct tccgagttca taccgccttt cttttttttg tattttcaat tgtccaaatt
    30841 tatcgtccaa aagtgcagaa cgaaaatcaa ttcatcacag gcatagcaga caattaaatt
    30901 tatacaaaaa aatgtaagcg aataatattc gtattgcctg aaaagaagta atatcaagat
    30961 ttttagcttg tttaaataat gaaattatct ctcttcgcgt attattataa ctcaaaaaaa
    31021 aggagtatgc aaacatacgt tcccttttct atatttgaaa ttaattattt cttttaacac
    31081 ttatgacgta aaaggctacg tttgtgattt ttatcataat attttaactt ataaattcta
    31141 ttaaaatagc tattattttg taatattcgt aatataatta gaataataaa aaatttttaa
    31201 ttttaatgtt actccatgaa gcaacaatca ctttgtcagt aaagtttcac tctaaccagt
    31261 ctttcgtaat agtgcttggg gagctttcat aattcaaacg tttttcactt gatactttag
    31321 cagctattgg tttttcgata aaccaatttt ttacatagac aaaaatcagg ttctggatat
    31381 tttcgacttt ttcggttttt aatttttgat aggcttttaa cagtgtcgtg tataattctt
    31441 gttctgcatt gacataataa ctatcgagtt cttcaaaaac tatcgaaata ccactttcta
    31501 tttcagcttt tttctttgca ttatgaatag tctggacggt tttttgagct tcactgtaac
    31561 ttgaagaaaa tgtttttatc aaatttaata ctcgttcagg gacaaacgta gcaatgctgt
    31621 cgtttgtcat aattgtctca aaattgtcta acaacaattg atcttgtagg cggtcttttt
    31681 cagtgtctat attgtgtcta ttagtgtcta atatattatt attaagttct ggtatgattt
    31741 tcataccctc tgatttatca acgttttggc acggtatgat tttcataccc tcttgagccc
    31801 caagggattc agcgtctgat ttttcgtcga gggtgggtat gattttcata ccctctgatt
    31861 tatcaacgtt ttccctattc tctctatcaa atttttcgag taagtagata tcattttctg
    31921 agacttttaa ttctgctaga tatagtctat taggattatt tttccctaac tgtttgttaa
    31981 atcccatctg cttttgtaat aataaaccaa aggattccaa ctcttttttg attgctatcg
    32041 ctttttgttt ttggcagttt aatacgctac atagttcatt tgtcgtataa gtaaaataaa
    32101 cattaccttc ttcatcgatt tgccgtttgc tgattgcgat ctctaatctt gcttttaaaa
    32161 gcatataggc aatcttagtt gaatctttca tttttttgta ttcgtcagaa acaataaaga
    32221 cttttggaaa ttgataaaat aattcgttat aaactctatc cgccttaaaa aaattaaaat
    32281 cagtcattgt agatcctcta ctttcacgtt gttttattta tgtaaaatta cctactcttt
    32341 tctaaaatag caaagataaa taaaaaatgc aaacgcatta aaactgtttt ttaattaaat
    32401 actacctaaa atcagaaata aaatgaatta tgtactacct ttaaagtcat taagaactac
    32461 tttaaataaa tattatttat aattaaatac taccagaaga acgtatgaat aaaatttagt
    32521 tttaaaatta aagataatta agtactacta gtgtcaaaac aaaaaataaa ttaagaacta
    32581 ccttatgcat ataaaacata ctaccattac tagtgatata tactacctta tttcagtagt
    32641 atatattata ttattcttat aagacaaggc tttaggctag aaaacaatag attatatact
    32701 actaaaaaat acaataagta ctaccaatgt cagatatata taataattat gtactaccat
    32761 taaaattaac ttatttaata agatactact gtgttaatct gaaaataatt actttaaagt
    32821 agttacatac tacctgttaa attagatact accattaata cttaaattag atactaccta
    32881 tatttatatt aagtactacc gttctaggaa taagataata aaaaaatact accaaattgc
    32941 aattaaatac tacttaaaat gtaaaaaata agaactaccc aaattaggta gttcttaata
    33001 agagaatatc agataaattt gtataagtca taaaaaaatt aatcttaaat ttttaaatat
    33061 atatacttgt t
//
LOCUS       DAZKCN010000021        30673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000021 DAZKCN010000000
VERSION     DAZKCN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.021"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..317)
                     /locus_tag="ACG8K3_000918"
     CDS             complement(<1..317)
                     /locus_tag="ACG8K3_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9144817.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKI"
     gene            complement(317..547)
                     /locus_tag="ACG8K3_000919"
     CDS             complement(317..547)
                     /locus_tag="ACG8K3_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9144818.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            834..1172
                     /locus_tag="ACG8K3_000920"
     CDS             834..1172
                     /locus_tag="ACG8K3_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144819.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1377..1939)
                     /locus_tag="ACG8K3_000921"
                     /pseudo
     CDS             complement(1377..1939)
                     /locus_tag="ACG8K3_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2671..3252)
                     /locus_tag="ACG8K3_000922"
     CDS             complement(2671..3252)
                     /locus_tag="ACG8K3_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9144820.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3435..4061)
                     /locus_tag="ACG8K3_000923"
     CDS             complement(3435..4061)
                     /locus_tag="ACG8K3_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9144821.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4083..5414)
                     /locus_tag="ACG8K3_000924"
     CDS             complement(4083..5414)
                     /locus_tag="ACG8K3_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP9144822.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5569..5970)
                     /locus_tag="ACG8K3_000925"
     CDS             complement(5569..5970)
                     /locus_tag="ACG8K3_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9144823.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6056..6262)
                     /locus_tag="ACG8K3_000926"
     CDS             complement(6056..6262)
                     /locus_tag="ACG8K3_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144824.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6357..7859)
                     /locus_tag="ACG8K3_000927"
     CDS             complement(6357..7859)
                     /locus_tag="ACG8K3_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP9144825.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7877..8332)
                     /gene="argR"
                     /locus_tag="ACG8K3_000928"
     CDS             complement(7877..8332)
                     /gene="argR"
                     /locus_tag="ACG8K3_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9144826.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8430..9047)
                     /locus_tag="ACG8K3_000929"
     CDS             complement(8430..9047)
                     /locus_tag="ACG8K3_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9144827.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9268..10641
                     /locus_tag="ACG8K3_000930"
     CDS             9268..10641
                     /locus_tag="ACG8K3_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9144828.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10716..11828
                     /locus_tag="ACG8K3_000931"
     CDS             10716..11828
                     /locus_tag="ACG8K3_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9144829.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11900..13537)
                     /locus_tag="ACG8K3_000932"
     CDS             complement(11900..13537)
                     /locus_tag="ACG8K3_000932"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9144830.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13705..14313)
                     /locus_tag="ACG8K3_000933"
     CDS             complement(13705..14313)
                     /locus_tag="ACG8K3_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9144831.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14697..15848)
                     /gene="queG"
                     /locus_tag="ACG8K3_000934"
     CDS             complement(14697..15848)
                     /gene="queG"
                     /locus_tag="ACG8K3_000934"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9144832.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15867..16619)
                     /locus_tag="ACG8K3_000935"
     CDS             complement(15867..16619)
                     /locus_tag="ACG8K3_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144833.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16760..17710)
                     /locus_tag="ACG8K3_000936"
     CDS             complement(16760..17710)
                     /locus_tag="ACG8K3_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP9144834.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17711..18550)
                     /locus_tag="ACG8K3_000937"
     CDS             complement(17711..18550)
                     /locus_tag="ACG8K3_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP9144835.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18547..19293)
                     /locus_tag="ACG8K3_000938"
     CDS             complement(18547..19293)
                     /locus_tag="ACG8K3_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP9144836.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19531..20901
                     /locus_tag="ACG8K3_000939"
     CDS             19531..20901
                     /locus_tag="ACG8K3_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9144837.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20956..21852)
                     /locus_tag="ACG8K3_000940"
     CDS             complement(20956..21852)
                     /locus_tag="ACG8K3_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9144838.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21966..22136)
                     /locus_tag="ACG8K3_000941"
     CDS             complement(21966..22136)
                     /locus_tag="ACG8K3_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144839.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22133..22810)
                     /locus_tag="ACG8K3_000942"
     CDS             complement(22133..22810)
                     /locus_tag="ACG8K3_000942"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP9144840.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22901..23806)
                     /locus_tag="ACG8K3_000943"
     CDS             complement(22901..23806)
                     /locus_tag="ACG8K3_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP9144841.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23981..25435
                     /locus_tag="ACG8K3_000944"
     CDS             23981..25435
                     /locus_tag="ACG8K3_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP9144842.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            25451..26482
                     /locus_tag="ACG8K3_000945"
     CDS             25451..26482
                     /locus_tag="ACG8K3_000945"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9144843.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26489..27676
                     /locus_tag="ACG8K3_000946"
     CDS             26489..27676
                     /locus_tag="ACG8K3_000946"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9144844.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27832..29214
                     /locus_tag="ACG8K3_000947"
     CDS             27832..29214
                     /locus_tag="ACG8K3_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9144845.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29186..29572
                     /locus_tag="ACG8K3_000948"
     CDS             29186..29572
                     /locus_tag="ACG8K3_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144846.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29772..30440)
                     /locus_tag="ACG8K3_000949"
     CDS             complement(29772..30440)
                     /locus_tag="ACG8K3_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9144847.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8618 a         6265 c         5569 g        10221 t
ORIGIN      
        1 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
       61 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
      121 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
      181 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
      241 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
      301 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
      361 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
      421 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
      481 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
      541 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
      601 tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa
      661 tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat
      721 tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt
      781 ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg
      841 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
      901 cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag
      961 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
     1021 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
     1081 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
     1141 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
     1201 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
     1261 ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga
     1321 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
     1381 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
     1441 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
     1501 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
     1561 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
     1621 ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat
     1681 gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt
     1741 taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc
     1801 ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct
     1861 tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa
     1921 aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac
     1981 aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag
     2041 tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat
     2101 tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca
     2161 ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt
     2221 tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga
     2281 ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
     2341 aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg
     2401 gcagcttttt cacaacctct ccactaaacg atgttcaatc agctaacctt cggtattagc
     2461 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
     2521 aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata
     2581 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
     2641 gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct
     2701 ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa
     2761 taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc
     2821 ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt
     2881 gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa
     2941 attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc
     3001 caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa
     3061 ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga
     3121 agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat
     3181 tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc
     3241 ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat
     3301 tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc
     3361 tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa
     3421 aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct
     3481 gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt
     3541 gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg
     3601 ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat
     3661 tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta
     3721 ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact
     3781 gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca
     3841 ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg
     3901 tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt
     3961 tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc
     4021 gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt
     4081 atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct
     4141 ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca
     4201 tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa
     4261 actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata
     4321 tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca
     4381 ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt
     4441 cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa
     4501 gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga
     4561 aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat
     4621 ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt
     4681 gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact
     4741 tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc
     4801 atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca
     4861 actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg
     4921 atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag
     4981 tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg
     5041 aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg
     5101 ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata
     5161 tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta
     5221 aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg
     5281 atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt
     5341 gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga
     5401 tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga
     5461 taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa
     5521 aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt
     5581 tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc
     5641 ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac
     5701 accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga
     5761 ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc
     5821 ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt
     5881 gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt
     5941 gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct
     6001 acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt
     6061 cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga
     6121 acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat
     6181 gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa
     6241 aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga
     6301 tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat
     6361 tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac
     6421 gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt
     6481 aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata
     6541 ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt
     6601 tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt
     6661 tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt
     6721 ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga
     6781 aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc
     6841 ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga aatataacga
     6901 acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc
     6961 gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa
     7021 ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta
     7081 tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc
     7141 gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat
     7201 tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca
     7261 gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt
     7321 ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct
     7381 ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca
     7441 atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag
     7501 ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg
     7561 gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct
     7621 ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca
     7681 atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt
     7741 gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca
     7801 actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata
     7861 taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt
     7921 cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt
     7981 cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca
     8041 atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac
     8101 gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt
     8161 tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt
     8221 gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag
     8281 gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca
     8341 cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt
     8401 acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc
     8461 gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct
     8521 ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct
     8581 ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt
     8641 ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct
     8701 tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt
     8761 tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt
     8821 ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt
     8881 attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg
     8941 tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt
     9001 tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg
     9061 cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt
     9121 tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac
     9181 gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt
     9241 ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct
     9301 gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat
     9361 gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta
     9421 acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc
     9481 aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg
     9541 atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca
     9601 gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat
     9661 cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc
     9721 cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt
     9781 gtgcccgttt tgattctttc tttgattatc ggctggtttg ctattcccga gatggcagta
     9841 caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga
     9901 agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc
     9961 ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga
    10021 ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag
    10081 tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt
    10141 agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct
    10201 cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg
    10261 attctttctt tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt
    10321 gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg
    10381 atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc
    10441 aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat
    10501 tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca
    10561 gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg
    10621 ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg
    10681 tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg
    10741 gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt
    10801 tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt
    10861 tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc
    10921 ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt
    10981 tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag
    11041 aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc
    11101 cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag
    11161 catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa
    11221 aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca
    11281 cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag
    11341 gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata
    11401 agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca
    11461 tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc
    11521 ctactagcga agcttataag ggattgacta agagtacaga acagttcttg actgatccag
    11581 atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt
    11641 tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag
    11701 gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata
    11761 ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc
    11821 catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact
    11881 tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg
    11941 cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa
    12001 gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta
    12061 taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca
    12121 cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc
    12181 cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat
    12241 agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat
    12301 ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac
    12361 caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt
    12421 tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg
    12481 cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt
    12541 tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg
    12601 ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag
    12661 caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac
    12721 cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag
    12781 ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa
    12841 agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa
    12901 agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat
    12961 ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa
    13021 aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca
    13081 aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa
    13141 attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat
    13201 acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga
    13261 tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa
    13321 catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc
    13381 ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat
    13441 taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg
    13501 tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca
    13561 aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg
    13621 aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg
    13681 tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc
    13741 agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc
    13801 agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc
    13861 gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct
    13921 tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg
    13981 tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt
    14041 tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac
    14101 ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt
    14161 gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac
    14221 ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt
    14281 tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt
    14341 tattctttcg acgctattaa ctttatcatt tagaaatatt tcataggtaa aagaaacatg
    14401 acatttaatg acaaaataac gaattattta atatttgtaa catttaaaca aatatttttt
    14461 tgatacaaaa ccataaaagc cttgttgtat aagcaaagta atcgtttttc tatgtttttt
    14521 tatttttgaa tgaaaaacgc agattctgta acattcactg caataatcca taaaaaaaag
    14581 caaaaaaatt tttgattttt atcaaattct cttaaaaaaa tgtaaaaaat acaaaaaaac
    14641 cagtaattgt tacaacaatt actggacaaa aactttgaat aatgttaact atttttttat
    14701 tttaactttc tccgcaataa tttctgggca tgttccaatt cgtttataac gacttcttct
    14761 gtttctttag ctaatatttc atctaataca tcaagccatt tttccggttc ttttgttcct
    14821 agacgtccaa tagcccaagc tgctgttcct ctgatgacag ggcggacatc tgttaaacat
    14881 tctgaaatct caggcaatgc cgttcgatca cctagattag ctaaagcaat caatgcgttt
    14941 ctctgcaaag gtttcttccc acgccatgaa ccagctaaat gcccaaattg ctgtttgaag
    15001 tctttattag acatcgaaag cattggcttt aattttggat atatttcgtc tacttttggt
    15061 tccatctctt catgaaaatg aaaatctttt ccttgattgt aagggcagac taactggcag
    15121 atatcacatc cataaatgac atttcgcatt tttttacgat attcttccgg catcatccct
    15181 tttgtctgag tttgataaga caaacatcgc tgggcattca ttcttccgtc accaagcaat
    15241 gcttttgtcg gacaagcagt cacgcagcgc atacagtcac cgcaaccaaa ttctcctggt
    15301 ttatcaggct caaatacaat attagtaacg atctcaccaa gatagacaaa tgaaccgaac
    15361 tcttcagtga tcaacaatcc gtttcgtcca ataaatccta aaccagctcg ctgtgctaca
    15421 gcgacatcga tcagttctcc agtatctact tgcggagcta gacgccattc ctcttctttt
    15481 tgccattttt ctgctcttgt ttggatgaat tcgatcaatc ggttcaaacg gtctctgagt
    15541 atatcatggt aatcgatccc ccaagaagca cgagcaaact gcccgcgttt ttcatcccta
    15601 ggcacttctt catggatcct agtaggatac gcaagtgcaa tggaaatgat cgatttcggc
    15661 tgatcaaatg tcatttctgg ataaagacgc tcatcgatat ttggatgttc aaagccagaa
    15721 gtatggccag ctgctttttg ttcttccaac gattctttta atgaatcaaa aggttccgcc
    15781 gtggtgaacc cgattttgtc aattcctaat cgtttgcttt cttgtatgat ttcttctttt
    15841 aatgtcatgg tcttcctcgc ttgctgttaa cggctgtata acccataata tagtttttct
    15901 tttttcagct ggacaaactg gatctgctga tcggcaatct tcttttcttc catccatttt
    15961 tttcttgcag caaatacttc ccaataattt gttgaaatta attttctttt gttatctaat
    16021 gcattaaaaa aattcaatga aactggtttt gtacttaaca gttcatggag ccttacctct
    16081 tcccatggcg aaaactcaga cagatcgcgg gaatacaata cttcttcata gagatttttt
    16141 tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc tagcaatttc tactagacga
    16201 ttataaaaca tattcattaa tttgctgttg gcaataaatt gacttttata ataggccgac
    16261 tggatctttt tgattactcc ttctgctttg atgactgaaa ataatgcagg ccatgctttg
    16321 acggttattt cgtcgttttg tttaactaat acgccaggtg tgaagccgac tggagaaagg
    16381 aacacaaaat aaccgatacc ggtattttca tctaaaggct gaaattcatc aaacaaccat
    16441 ttgcctatgt acagctcttc atctggttct gttgtctcat tttgatttgc tatgaataat
    16501 ttagctaatt ctgcttcaag aaattgaagg tcttgatcct tgtcggtatt ttcctgctgg
    16561 ctgatcaatc gaaagacttc ttctttaatc gtatatttat ctttacgtgt aaaattcata
    16621 gccacacctt ccttccttac tctaactata acggaatttc ttcaaaaaag gtatctgtac
    16681 gacgaaaaac agcataattt tttttcatat ctcttataaa aaatgcaagg tctgctcctc
    16741 aagctagacc ttgcactttt cagctttctt ttgtttgtga caaaccttca tggatcgttt
    16801 caatattcca tttcatcatc tgatagtaag aatctccttg ttctccttct tttgctatgg
    16861 aatcagtgaa cagtttatca tagattttca aacccgtttc ttttgctacc cgttccatgc
    16921 ttcttgagtc tacactggtt tccacgaata aaaccggtac ttcttttgct cttatctgat
    16981 caatgatcgc tttcatttga tcaggcgtac cttgactttc tgtattgatc tcccatatat
    17041 aagctgctgg caaatcatat gctcgtgaaa aatacttgaa tgccccttca cttgttacta
    17101 ataatttttt gttctcttcg atagaagcaa aactttcctt cgcttgtgta tctaattctt
    17161 ttagtttttc cacatatgct tctgcatttt ttttatagag tgtcgcattt tctgcatctt
    17221 tttcagagaa tatacgtgcg atttcctcca catattttat tccgttagat aggtctagcc
    17281 aagcatgggg atctgctttt gtatgttctt caccgctagt taaataaaga ggttctacat
    17341 ttttgctaac tgcaaaataa tcttttcctt cttctttttt agccgtttcc atcaagttat
    17401 cgaaccagct gttacctgtt tcaagattca aaccgttgta taaaataaca tctgcatcgc
    17461 ttgccttttt gatgtcttct ggtagtactt catattcatg cggatctgtt ccgactggta
    17521 cgatactgtg gatatctatt tgatctgttc cgacctcttt tgccatgtcc gccaagatcg
    17581 aattggtagc cacgactgac aacttttctt tacttccttc atccgcttgg tcagaagcct
    17641 gctttccgca accggctaat accaagaaac taactgccaa gattaaaaag agtgattttt
    17701 ttgttttcat ctatctgctt cctttctgaa ataaaattcc ttttgttgga gaaaataaaa
    17761 aagcaaggat aaaaaatgcg gtacatgcta acacgatcgt agctcctgat ggccaattgt
    17821 aactataact gaaaaacaca ccgacaactg cacttagcat accgattcct gcagctgtaa
    17881 tgatcatttt cagtaaatgg ttcgttagca agtaagccgt ggcggctggt gtgatcaaca
    17941 tggcaatcac caagattgtt cctactgtct gtaaactgac tacagccact aatgtcaaaa
    18001 agaacatcaa aagataatgg atcagcgatg tgttcagccc ataagctttt gccattgtcg
    18061 gatcgaatga tgtgatcttc aattctttat aaaagaaaaa gacaaatatc aagacgattc
    18121 cactaacgag tgctgtcagc aacaaatcgc tttccctaac agctaagaca ttcccaaata
    18181 aaatatgata caaatctgtc gcactctggg catatgaaat caagataatc cctaatgcaa
    18241 aaaaggagct aaagacaatt ccaatcgctg tatcattttt caatcggctc cgctgagtca
    18301 cgaaaccaat cactgctgct gttagcatcc caaaagcagt ggctccaaag atgtaactaa
    18361 aaccaaacat ataagaaatg gctacacctg gaagaactgc gtgagagata gcgtctccca
    18421 tcaaagacat ccctcgtaaa ataataaaag aaccgatgac tccagaagca agtcctacaa
    18481 gcatggaagt gaccaaagcg tattgcagga attgataatt cattaatcca tcaataaagg
    18541 attggatcat tttttctctc ctccttttac taaatgtctg atgatctcac cataggccaa
    18601 ttgaaggttc ttcgatgtaa agacttcttg tactggaccg gaagcgatca gctgtttatt
    18661 taaaagaata acctcatcga aatattcttc tactttatga agatcatgat ggacgatcaa
    18721 aatcgttttt cctgaattct tcaattcctg caagatgtca aagatctttc gttcacttaa
    18781 cgcatcaatc cctacgaatg gttcatctaa aaagatccat tctgctcctt gggctagagc
    18841 tcttgcaata aaaactctct gaagctgtcc acccgatagt tcgctgatct gtcttttggc
    18901 atattcttcc atccctactt ttttcaaagc ttgtcttgca cgttcttttt cttttttccc
    18961 aggtctttgt ccaattcgta aagatggata tgttcctaaa agtacaacgc ctaatacatc
    19021 aattggaaag gaaagatcca attcacttcg ctgttccacg taggctattt ttttacggac
    19081 tgagtttacc gactgtccag aaaaggtgac tttaccagta gtagctggta tcaactctag
    19141 caaagatttc ataaatgttg attttcctgc accgtttggt ccgataatac cggtaatttt
    19201 ttgtggattt atcgtcaagt tgatagtttt caaaacagtt ttcccttgat aagcggctga
    19261 aaggtcttga attacaatgg ccgtacgttc caaatcttac actccttttt aggtttacct
    19321 aactttttat ttaaatatag tttaactaaa atggaaagca ttttcaagta gaatgatgaa
    19381 aaagtttttt tcgaattttc ctcgcatttt atgaaaattt tctgtagaat cacatcaaga
    19441 gttcaataag gctaacctaa taatgaaaaa catgtgttga aacgtttgat tattttttct
    19501 tgttgactca acaacgaaag gagtttttgt atggaagaag aaaagttaac tcgaagtctt
    19561 agtgcccgtc atatccaaat gatcgcacta ggtggaacga tcggtgtcgg tctgtttatg
    19621 ggtgcctcct caaccatacg ttggacaggg ccctcagtga tgggagccta tgcgattgca
    19681 ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa tgctttacgt tgatccttcg
    19741 actggttctt ttgcaaatta cgcgagcgag tacatccatc ctgtggctgg ctacttgacc
    19801 gcttggagta atatttttca atatattgtc gtaggaataa gtgaagtgat tgctgtagga
    19861 tcttatatga attattggtg gcctgatttg ccggcaatta ttcccggaat cattgtcgtc
    19921 ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt ttggcgaatt agaattttgg
    19981 ttttcgatca tcaaagtcat aacgatcatt ttgatgatca ttgcaggtct tggggtaatc
    20041 ttatttggat ttggtaatca tggtcaggca gtagggattt caaatctttg gaaaaatggt
    20101 ggattcttca ctggaggcgt caaaggattt ttctttgcat tatcgatcgt tgttgcttcc
    20161 tatcaaggaa tcgaactgat cggaatgact gctggtgaag cagagaatcc taaaaagacg
    20221 atcattgaag ctgtccagtc cactatcggt cgaatcttga ttttttatat tggtgctatt
    20281 tttatcattg tgagtatcta tccgtggaat caattgagtg agattggttc accttttgta
    20341 caaacttttt ctaaagtcgg cattacattt gctgcaggac tgattaattt tgtcgtgatt
    20401 acagcggcat tatcaggttg caattcaggg attttcagcg caagtcgtat ggtctataca
    20461 cttgccataa atggaaagat gtctaaaaaa tttctgaaac tatctcgtca cggtgcgcct
    20521 ttttatcctg tcgtagcaat ctcaggtgga atcttaattg gtctgatctt aaattatctt
    20581 ctaccaatgt tcatcaacca gtcagaaaat attttcgttt tcgtatacag ctcaagtatt
    20641 ttgcctggta tggttccttg ggtagtgatt ttgatcagcg agattcgctt caaaaagaag
    20701 cacgcagata aaatgcatga tcatcctttt aaaatgccac tatctcctta tacgaattat
    20761 gtgacacttg cttttttagc tatcgtattg attttcatgt ttatcaatcc tgaaacgaga
    20821 atctcattga ttattggact cgttttcttg ctttacatga cgatccatta ctttgtacgc
    20881 gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac cttagtggaa gcttcgttct
    20941 tttttattgc ttttatcaat tcaattttag cgtctcaccg ggagtcagga aataattctc
    21001 catgctcatc ccattcagcg acaagatcgt ttctactggt acgccagttc gttcggctat
    21061 ttgatttgga ccttctcctt ccaaaactac ggttgtcgca gcctgtgaag tcgcgctgtt
    21121 ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt cgagcttgca tttcagaagc
    21181 ttctaccgac tctgctgtct cagaatcaga agatgttgtg tcttgtgtgc ttgttgactc
    21241 tgcttgaaga gaggcgctag tctgcaaata aatatcggaa atcttagcga catgattcgt
    21301 ataattagcc gataattgat tgatcaaagt atctaagtct acaccgtctt tattttctgc
    21361 tgcctgatac gctgcacgta attttctgat cccttcgtcg gagattgctt gaagctcacg
    21421 tgctttttgg ttagcaactg ctgataaacc agctattccc tcttgatttc cctgaatttc
    21481 tgtctggtat tcttcaatca atgcaggcgt cttttcttcg atctttttcg aataatcatt
    21541 tacgattgct tgaagttgct ctggcaaacc atttgctgtt ttttcttcag aagtttgtgt
    21601 ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc tctgttttct ttgtcgttgt
    21661 ttccgttatg gtcgtacttt tcgcttctga cgaatttttt ccatgaaaat atagacccgc
    21721 aactcctcct gctatgatca aaaacacggc aactgctgcc ccaatcatga tcatccgctt
    21781 acgagctgcc tgtttttgct gtcttcttcg ggtcatccga acattttctc cccactcatt
    21841 ttcatcatac atttttttca ccaccaattt ttatgtataa tagcgttatc aaacgcttca
    21901 tattcttatt gtagcaaata attgtcgctg tgcacatttt tatctttgtt atttataaga
    21961 aaatttcacc agttgagatg attccaagat tgtccgtcga tttctccttg cagctgcatt
    22021 gttcgttcgt ctatctcagc catcagtttc tttatttcat ataagagcgc acggtcttga
    22081 tattctgatt tttcgcttaa ttcatctaat tgttggatca gccaatcatt tgtcataatg
    22141 aagttccttt catttcttgc agtgtaaagg tcaagtcttc gatctgctct tttgcctcag
    22201 tcagtgcttc tacgatatat gcttgttttt cagattcttc ttcctgcatt ttctgatttt
    22261 cctgtactct ttgaataaac cagtcaatcc gctgagtcag cgatggtttg ccttccaatg
    22321 agagatactg ctgataagca attagcttct cccaacgttt ttcctgggat aaatacataa
    22381 attgtgctaa aacgaatggc ggaagatagg tcatatggaa tttatttgcg attcgctgat
    22441 acggcctcaa aaattcggaa atcgtatatt cttcttttcc tccagcttgg tattcttttt
    22501 ccgccacacc tgttgtcaat acgattccta gttctttccc ttttaataca tgtgctgctc
    22561 gttccaagac attatcttgc caaattttta aatgagcagg agaactatac cagtaaaacg
    22621 gaaactggaa aatgatccga tcatgctggg ccaagagctg tagttcagtc tctatatcta
    22681 tttttccgtc tggataactt gattccaaat gatgccaagt agctggtaaa ccagcagaag
    22741 cttctttgaa aaattgctga tttaaagatt cttcgatcgt tggatggcta atgatgataa
    22801 gtgtacgcat cccttttcct cctttttagt cttcgcttta cagtgtagca taaccgccac
    22861 aagtttcttt tgccgatgct tgtcatttta accagactaa tcaggttgtc caagaaactg
    22921 cggtttgttt ttccaactgt tttttcaagg tatccaaagc aatattctta aaattttcgt
    22981 ttttcatacg tttacgatag ttcgtagccg tttctccggt actctttttg aacattcggc
    23041 taaatacgac tagactattg aaccctactt gataggcaat actttccacg gattcatccg
    23101 tcagagaaag aagaaacgcc cctcgattga tccgtacccc gatcaaatat tccttgatac
    23161 tggtacccat atgatttttg aatactcgcg acaaatggct ccgattgacc gctaaatgct
    23221 gggcgatttc ctcaacagac aatttttcag cataatgtgc ctcgatgtat ttgactgttt
    23281 caacagctaa agaggaatga accttttgta caccaccgct gacctttccg ccatctttta
    23341 aaaggactga taaaaaacgg taggttaatg ctgttaactg taattcattt tccaaggttt
    23401 cttccgtatc tttcatgcat tctaaaataa tgtcgatata tttttgtatg tcagaagaga
    23461 ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat gatttcttct gcagcactcc
    23521 caccaaatga aagccagcaa tacatccacg gatcttctcc atctgataag taaaacgtcg
    23581 agtctcctgg acgaatcaag aacagatcac cttttttcaa ttggaattgt tgatttttca
    23641 catgataaga tcctttgcct tccatcacga catgcaggat gtaatcattg cgaatagcag
    23701 gaccaaaaga atgaaacggc atcgttttag agaaaccaca aaaatcaaaa tacaattctt
    23761 tttctacttg ttgatgttcc ttttttaaaa aaatcgattg ctccacgtct atccctcctt
    23821 actcctttag tataatgcaa caaaaagtta aataaaagtc acagaaatat attaaaaagg
    23881 aagcgatttc ttgttaagtt taagtcatta aaagatcttt gttattcgtt gtaaccggtt
    23941 tacttagtat tatatattga acaggagagg aatacagatt atgaaaagca aaatgatcca
    24001 acgtgtgtcc tacgctttag gcgcttttgg acacgacaca ttctatgtta cattaagcac
    24061 ttattttatg gtattcgtga caagccagat gttcgcagga acagataagg caacgaatgc
    24121 aaaaatgatt gctacagtta caagcttagt tgtaggtatc cgcttagttg aaattatttt
    24181 tgatcctttg attggtggca tcatcgataa tactcgtaca aaatacggta aattcaaacc
    24241 atggttagtt atcggcggcg ttgttagttc cgtactcttg gtagcaatct ttaccaattt
    24301 ctttggctta gctactcata atacgacttt atttatcata ttatttgcaa tcgttttcat
    24361 cttattggat agtttttatt catttaagga tatcgctttt tggtcaatga ttccagcttt
    24421 gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt gctcgtttcg gctcttctct
    24481 aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt actttcttta cctatcttgt
    24541 tactcataaa catattgaag gcgcttcagg ctggttctgg ttcggtgtga ttgctgctgt
    24601 tatttcaggc ggtactgcga ttatcactgc cttgggcaca aaagaagtcg atactgcgat
    24661 ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc aaagcagttg ctcaaaatga
    24721 tcagttgatg tggcttgccc ttgcttatat cgcttatgcg attgccaatg tagcaacaac
    24781 tgccgtatta ttctacttct tcaaatatgt acttggccaa ccaactgaat tttggatcgt
    24841 tggtctagtt gctgccttac ttggtttagt agctgttcca ctattcccaa tgcttacacg
    24901 cgtcatctct cgtcgctacg tttaccttgg agggatcgca atgatgattt cttcttactt
    24961 tttctttacg atcgcaggtt caagtttgcc aatcgttatc atcgggctag tattgttcta
    25021 cttccctcaa caattgattt tcttatccgc tttgatgacg atcacagact ctgttgaata
    25081 tggtcaatgg aaaaatggta tccgtaacga agctgttact ctatcaattc gccctttact
    25141 agataaaatc gctggtgctt tgtctaacgg gatcgtaggg tttgtcgcta tcgcttctgg
    25201 gatgactggt aatgctactg ctgctgatat cactgctcat ggcgtaacga ctttcaaagc
    25261 ttatgcattc tatgctcctg ctgctttgat gattttctcc gctattattt ttgcttggaa
    25321 gattacattg acagagaaaa aacatgcaca aatcgttgag aaattggaaa acaaattggc
    25381 tccagctgat acaatggaag atgaacttca cgatgcagta gttccttccc attaagaaag
    25441 gatcttgaaa atgaagatta cagaaaaaga ttttggccaa ggttatcact tgataacctt
    25501 ggagaacaaa aacaagctag cactttctat ctctgattta ggtgctagaa tcgtcagttt
    25561 aaaatcaaat gatcgagaat tagttttggg atttgacacc gctgaagaat atatcgaaaa
    25621 agatccgtat atcggtgctt ctatcggtcg caccgcaggg agaatcgaaa atggccactt
    25681 ttctctcaat ggaaaaactt accaattagc cacagatcca aaaactggac ataatctgca
    25741 tggtggtgct cctggatttg aattaaaaaa atggtcttat gtcattttga atggcgaaaa
    25801 tgaagcttct gtcattttta ctactacaag tcccgacgga gaacatggtt ttccaggtac
    25861 aatggatgtg gaaatccgct atactttgac aaaagataat atctggcgag tgacctctcg
    25921 cggcaccagt gaccaagata ctttattcaa tccgacaaat catgtttact tcaacttgac
    25981 gggagatgct tctcagtcaa tcgatcaaca tgagctttgg ctcaatagcg aagcttatgc
    26041 tcctttgcga acagatagta ttcctatagg ggtaaaagaa aatgctgcag gaagtgcttt
    26101 cgattttcaa atacctaaaa aattagcgtc cgtttttgct agtgatttgg atcaaaaaaa
    26161 tcttgttgac ggaatcgatc atccattttt cttaaaagaa accggcttgg gtaaagaagc
    26221 cgctcgcttg actagccccg ataaaagaat ccaagtagat attgctacag atgcttctag
    26281 tgtggttatt ttcactgcaa atttcggcac agaaacacca gaaatgcgca accgaaaact
    26341 ggcacatcac ggtggcatca catttgagac tcaaacagct ccaggtgcag agcgcttctc
    26401 agcatttggt tcgattcatt taaaagccgg atcagtattt gaaacagtga cagaattcaa
    26461 aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa agaagcttaa acacaaaatt
    26521 tgccgaaaca ttcggaccta aaaaaactac acaatacttc tcaccaggac gcatcaattt
    26581 gattggtgaa cacacagatt acaatggagg ccatgtcttc cccgcttcca tcacttatgg
    26641 aacgcaaggt gtagcagcac ctcgagacga taataaaata ttagtttatt ccacaaactt
    26701 tgacgatatc ggtgtcatta gttttacttt agatgagcta gaatatgata aaaaagcaaa
    26761 ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa gaagctggac actcaatcga
    26821 tcatgggttt gagttattag tggaaggaac aatccctaat ggtgcaggac tttccagtag
    26881 cgcttcatta gaattattag taggtgttgt attagaagat ctgtttgatt taaaaacaga
    26941 tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat gaattcatcg gtgtcaattc
    27001 aggtatcatg gatcagtttg ccatcggctt cggtgagcta gatcatgcaa tcttattaga
    27061 tacaaatacg ctgaaatacg aaatggttcc agtgaaacta gatggttatg caatcgtcat
    27121 catgaatacg aataagcggc gggaattggc agattcaaaa tacaatgaac gccgtagcga
    27181 atgcgaagaa gccttggcac gtttgcaaaa aactttgcct atctcttctt taggagactt
    27241 agatgaagaa gaatttgaat caactattga ccaaatcggc gatgatacat tgatacgcag
    27301 agctcgccat gccgtttctg aaaatcagcg gactttgaag gcaaaagctg aattagaagc
    27361 tgggaactta gaagcatttg gtcaactgtt aaatgattct caccactctc ttcgctatga
    27421 ttatgaagta actgggatcg aattagatac actagtagat gctgctcaaa aacaagaagg
    27481 tgtcttaggt gcacgaatga caggagctgg atttggtggc tgtgcgattg ctcttgtcaa
    27541 agaagaaaat atacctgcct tcaaaaacaa tgtctatgat gaatacatga aagttgtcgg
    27601 ctatgctccg gaattttatg ttgcacacat tggaaatggg acaacaaaga tcgatccaga
    27661 agctgttcat gtttgataaa aaaatcaacg atctcagcta aaaacaaata gataaaaata
    27721 acctctcagc aatttccaaa tgtgagcctt ttgctcgtat ttgaaaattg ctgtttttta
    27781 ttgacacgga aagagaacgt gtgttctaat tgtgttgaag aggtgattgt tatgtgggcg
    27841 aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa gagatatttt atgtattgac
    27901 tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg atcttgatcc ccttacaact
    27961 aaattagtag tcatgtccta tccgtccgat tcgacagaaa cacggggtag cggattaatc
    28021 ttggcttcca gtcctgcagc aaaaaaagct tacggcatca ccaatatcag ccgtgccaga
    28081 gatcttccct tcccctatcc aagtgattta tatattgttg ccccaaggat ggcctattat
    28141 atggaaaaga acagagaaat caattctatc tataaaaaat atgcagacga agcgaaccat
    28201 catgtctatt ccgtcgatga aagcttcctt gatgtaacaa atagcatggc actctttggt
    28261 aaaacaaatg cgtacgacat ggctaaactg atccataaag atgtgtttga gcagaccgga
    28321 atctacacga ctataggaat tggcgataac ccgctacttg ctaaactagc cttagacaat
    28381 ggctcaaaag acggaaagca aatgtttgct gaatggcgtt atgaagatgt accagaaaag
    28441 ttatggaaaa tctcagatat cactgacttt tggggaatcg gcaaacgaac agcaaaacgt
    28501 ctgaaacaaa tgggtataca ttctgtttat gatttagctc acgcggatta ttatcacttg
    28561 aaaagcaagc taggggtcat cggtactcag ctttatgccc atgcttgggg catcgatcga
    28621 agttttttgg gacaagaata tacaccaaaa tcaaaaagta tcggaaatag tcaaatccta
    28681 gatagagatt atacaagacg agaagaaatc gagatcgtta tcaaagaaat ggccgaccaa
    28741 gtcggcactc gcttgagacg atctgcagca aaaacacaag tagtcagctt atgggtagga
    28801 tactctttag gctatcttga tccaaatggc aagaccgggt ttcaccagca aatgaaaatc
    28861 gaagcgacaa atgctagcag agatctagca gatgctttat tgaccatttt ccaccagcat
    28921 taccactacc aggatattcg gcgtgtaggc gtgaactgtt ccgacctcgt atacgcgaca
    28981 ggtttacagc ttaacctttt tgaagatcct gaaaagcaga taaatgaagc gaaagttgat
    29041 tttgttgtcg atacgattag aaaaaaatac ggctttcaat caatcatcca tgcgcatagt
    29101 ctcttgcaag gaggacgcgc catcgcacgt agttctctcg ttggcgggca tgcaggtggt
    29161 atgagtggat tagaaggtgg aaaagatgag acggacagca aaaaaattca ttgactataa
    29221 cgagtatcga gatcggccat ttggattaaa atgggggact gcttttgcga tggaagaact
    29281 agtcaacggt atcaaaaaaa atgaagaaga agcacgcaaa cagcctcttc ctaaacaaca
    29341 aatgacattg caagaaatcg atagtatact gacagaagcc tttttatctc gtaaagcagt
    29401 aatcattcag ctcaatttaa aagatgaatt tggaagactc ttggatgagg tcgaagggca
    29461 cttcattggt gaagcctatg aagattactt tatgattgac catcagccga tattgtggga
    29521 agacgtacgg tatattgagt taaaggaaac aaaaaaatgg tttgtgacat gagaggttgt
    29581 gacagaagtg atcaacttca aaaaataaga aaggattttt cgaaaattgc ttcttaaatt
    29641 tttgtggaat tttggcttat ttttcgaaga agcaacttct ggaacaacgt atattagata
    29701 tcagaggttg tgaaaaagtc gcttagtttt gagtaacaag ccaaggcgct gatttcacaa
    29761 cctctgtcta tctatccatc tattcggtaa gattcatgca tgacgtcttc tttatttagt
    29821 atgtagagcc ctttttcttc tgaccattca acataaatca gctgttttac atctgcatag
    29881 ccttctttct tcaagttctc cataagccat gcttcagtca attgattgga ctgaagcact
    29941 ttatgctgaa tttgcccttc atctacaagc aggatcgata aagctttgtc ttcatctgat
    30001 tttttcaata cactcatttg accggcattc tctaggataa catgtttggc attttcaaga
    30061 aaaaagcatt cttgctgtct taagattgct cgcaactgct ccatctcgat atggttattt
    30121 gtcaactctg tcatgttgat gacgccgtct ttgattagaa cagatggctc gcctttgacc
    30181 caacgattga ccttttcatt tttttggacg atacgttcga taccataaat cattactgcc
    30241 caaagcgcaa tggcaaatag gacatgccct acatggacat tgtcatcata ggttgattct
    30301 tctaagatcc cacctaaaac aagcgtatag actaaatcaa acggtgtgat ttctgacatg
    30361 gatttcttcc caagcaaacg taccactaat aataaaccga tcaacccgaa caccagtttc
    30421 attgtaattg aaaaaatcat tttttctccc cctaactatc tgttaattaa attatatatg
    30481 ctttctcacg aacacgcatg gaatatacaa ggaagaaatg tatcctttta ctaaaaaaag
    30541 gactgtgaca ctgtagtgac ccccaaaagt tagacttttt tggagtggag aaatccactc
    30601 ctttttttta tgctctaaaa tgatattttt acacaaccgt tacggttgtt ttacgaaatt
    30661 ggacgagact ttg
//
LOCUS       DAZKCN010000022        30386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000022 DAZKCN010000000
VERSION     DAZKCN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.022"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..2191)
                     /gene="glyS"
                     /locus_tag="ACG8K3_000950"
     CDS             complement(116..2191)
                     /gene="glyS"
                     /locus_tag="ACG8K3_000950"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9144848.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2193..3110)
                     /gene="glyQ"
                     /locus_tag="ACG8K3_000951"
     CDS             complement(2193..3110)
                     /gene="glyQ"
                     /locus_tag="ACG8K3_000951"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9144849.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3496..4308)
                     /gene="recO"
                     /locus_tag="ACG8K3_000952"
     CDS             complement(3496..4308)
                     /gene="recO"
                     /locus_tag="ACG8K3_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9144850.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4451..5350)
                     /gene="era"
                     /locus_tag="ACG8K3_000953"
     CDS             complement(4451..5350)
                     /gene="era"
                     /locus_tag="ACG8K3_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9144851.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5365..5760)
                     /locus_tag="ACG8K3_000954"
     CDS             complement(5365..5760)
                     /locus_tag="ACG8K3_000954"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9144852.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5744..6220)
                     /gene="ybeY"
                     /locus_tag="ACG8K3_000955"
     CDS             complement(5744..6220)
                     /gene="ybeY"
                     /locus_tag="ACG8K3_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9144853.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6237..8429)
                     /locus_tag="ACG8K3_000956"
     CDS             complement(6237..8429)
                     /locus_tag="ACG8K3_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP9144854.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8453..9424)
                     /locus_tag="ACG8K3_000957"
     CDS             complement(8453..9424)
                     /locus_tag="ACG8K3_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9144855.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10130..11509
                     /gene="brnQ"
                     /locus_tag="ACG8K3_000958"
     CDS             10130..11509
                     /gene="brnQ"
                     /locus_tag="ACG8K3_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9144856.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11665..12111)
                     /locus_tag="ACG8K3_000959"
     CDS             complement(11665..12111)
                     /locus_tag="ACG8K3_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP9144857.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12138..12314)
                     /gene="rpsU"
                     /locus_tag="ACG8K3_000960"
     CDS             complement(12138..12314)
                     /gene="rpsU"
                     /locus_tag="ACG8K3_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9144858.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12476..12904)
                     /locus_tag="ACG8K3_000961"
     CDS             complement(12476..12904)
                     /locus_tag="ACG8K3_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9144859.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12996..13856
                     /locus_tag="ACG8K3_000962"
     CDS             12996..13856
                     /locus_tag="ACG8K3_000962"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP9144860.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13950..14300)
                     /locus_tag="ACG8K3_000963"
     CDS             complement(13950..14300)
                     /locus_tag="ACG8K3_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9144861.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14316..16043)
                     /locus_tag="ACG8K3_000964"
     CDS             complement(14316..16043)
                     /locus_tag="ACG8K3_000964"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9144862.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(16257..17183)
                     /gene="trxB"
                     /locus_tag="ACG8K3_000965"
     CDS             complement(16257..17183)
                     /gene="trxB"
                     /locus_tag="ACG8K3_000965"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9144863.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17328..18761)
                     /locus_tag="ACG8K3_000966"
     CDS             complement(17328..18761)
                     /locus_tag="ACG8K3_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9144864.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18761..20164)
                     /locus_tag="ACG8K3_000967"
     CDS             complement(18761..20164)
                     /locus_tag="ACG8K3_000967"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9144865.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20394..21296
                     /locus_tag="ACG8K3_000968"
     CDS             20394..21296
                     /locus_tag="ACG8K3_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9144866.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21337..23223)
                     /locus_tag="ACG8K3_000969"
     CDS             complement(21337..23223)
                     /locus_tag="ACG8K3_000969"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9144867.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23320..24171)
                     /gene="licT"
                     /locus_tag="ACG8K3_000970"
     CDS             complement(23320..24171)
                     /gene="licT"
                     /locus_tag="ACG8K3_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9144868.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24506..26290)
                     /locus_tag="ACG8K3_000971"
     CDS             complement(24506..26290)
                     /locus_tag="ACG8K3_000971"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144869.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26290..28041)
                     /locus_tag="ACG8K3_000972"
     CDS             complement(26290..28041)
                     /locus_tag="ACG8K3_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP9144870.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28183..28407)
                     /locus_tag="ACG8K3_000973"
     CDS             complement(28183..28407)
                     /locus_tag="ACG8K3_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP9144871.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28658..30244
                     /locus_tag="ACG8K3_000974"
     CDS             28658..30244
                     /locus_tag="ACG8K3_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9144872.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8470 a         6566 c         5440 g         9910 t
ORIGIN      
        1 agaataaaat aacaaaaatt attaaaatag aattaataac agctatttat atttataaca
       61 aaaaaacaga gacacagcac gtttcgtgca tgctctctgt ttttcaaaca aaagactatt
      121 ttgtgatgat ttgatccaag cttgctaatg acaaagccat ggcagctatt tgactaagtt
      181 gtgttagtcg gttcttacgt actgctggtt catcagccat gaccatcgtt tgatcaaaat
      241 aattttcgat cagtggacga agagcgacaa gctggtcgta gttttcgtgg atcgtttggt
      301 cagcaaaact tgctttcatt gcttctactg ctttgtgtaa ttctttctct gcgtcgtttt
      361 cgaacagctc aggatctact tctgattgtt tttcaagctg cgcttttttc gctaagttca
      421 cgacacgtgt caaagcttcc attgaaggtt tgaagtcttc atctgcaaga tgttgtttga
      481 acaattgtgc tgctgcaaat acttttgtca gatctttttg ttccgcattc aaaacagcat
      541 cgatgacatc atgacgaata ttcttcgttg tcaataattg gcgcagacgg cctttgataa
      601 agtcgatcac ttgttgttgc ccttctgata aaccaatgcc gaaacgatcc gtatctttac
      661 tgataacatc gctaatcgtt tcttgcagaa cagacaaagg aaaggcccat tttttcgatt
      721 cgatgatccg aacgacaccg tatgcttggc gacgtaaggc ataaggatcg tttgaaccgc
      781 ttgggatcat cccaacagca aagaaagaaa agatgctatc taatttatct gcaatcgcta
      841 aaacagaacc tactgtagac tgaggtagct ctccttcact ggatataggc atgtaatgct
      901 cacgaatcgc tgtcgctaca gcaggtttct cgcctttcag cagagcatat ttttcgccca
      961 tgatcccttg cagttcagga aattccccta ccatgttggt tactagatca aatttataga
     1021 tttcagcagc tcgttgcaga tccatcaatt cttcctcaga tagtcctact tgttcgccaa
     1081 tgatacgggc aatgacttgt acccgttgca ttttctcata gatactaccg attttttcat
     1141 ggaaagtaac agtttttagt ttttcaacgt attggtcgat tgaagctttc ttgtcttcat
     1201 tataaaagaa ttccgcatct tctaaacgag ccgtcaatac tttttgattg cctcgaacca
     1261 cattttccaa ttttacccga tcaccattac gagcagaaat aaaatgagga agcagcatac
     1321 cttgatcatt ccgtacctcg aaataacgtt gatgttcttt catagaagtg actagtactt
     1381 cttcaggaac agaaagatat ttttcttcga agtcgccaga aaagacagtt ggatattcga
     1441 ctaaattgtt gacttcttct agtaaatctt cgtctagatc aataacccaa tgattttttt
     1501 cggctaattc attggcttgt gtaacgatca tttcttgtct tttttgcgga gacgcaatga
     1561 cgaattgttc attcaatttt tcttcatatt cttttgcatg cgcaaatgtc acatcttcgc
     1621 ctaagaaacg atgacctcga gaggctctgc ctgttttgac atccaagatt tcgaatggga
     1681 ttacttcgtc atctaacaat gccacgatcc agtgaatagg gcggatatat tcaaaatcgt
     1741 attctgccca atgcatcgtt acagggaaag tcaggctttt aatgacatct gctaaacctg
     1801 tcaatacttc tttggattcc ttaccatgag tgtatttcgt tacatagaca tattccacac
     1861 cttttaattc tctaaaagtg atagcgtctg tcgtcagacc ttgtccgcgg acaaatcctt
     1921 ccgctgcttt cgtccagttt ccatctgcgt ccaaagcaat ttttttggct ggtcctttga
     1981 cttcttcttc actgtccgct tgtcgttctg gaatgtttgt tactttgatt gctaaacggc
     2041 gcggtgtgga aaatgtttcg atcgtatcat aagtcaattg gttttcatct aaaaattttg
     2101 ctgttttttc ttctaattgt ttcatgcttg gtgtgacaac atgagcaggc atttcttcca
     2161 gcccgatttc aagtaataag tcttttgcca tcttattcag cctccttcac gttttctgga
     2221 tttgctgtgt ttttattcaa caaagggaat cctaattttt cgcgttctgc cacaaagatt
     2281 ttggctacag cacgagccat gttgcggatg cgagaaagat aaccggcacg ctctgttacg
     2341 gatactgcac cgcgggcgtc taataagttg aatgtatggc tacatttcaa gatataatca
     2401 tacgccggat gaacaagatt ttcatcgata cagcgtttag cttctttttc aaaacgatca
     2461 aagttgctta gtagcaagtc ttggtcgctt acttcaaaag aatatttaga atgttcatat
     2521 tctggttgtt tgaagatctc gccatatttc acgccatcag cccattctaa atcatagaca
     2581 ctttcaactt cttggatata agaagcaaga cgttctaatc cataagtgat ctcagaagtt
     2641 actggtttac aaggcagtcc accaacttgt tggaagtacg tgaattgtgt gatttccatt
     2701 ccgtcaagcc atacttccca acccacaccg gcacagccca tggatgggtt ctcccagttg
     2761 tcttcaacaa aacggatgtc gtgttctagt ggatcgattc ctaaaaggcg caagctttct
     2821 aaataaagct cttggatatt ctcaggtgaa ggtttcatca cgacttggaa ttgatgatgc
     2881 tgatacaaac ggtttgggtt ttcgccataa cgtccatcag ctggtcggcg agatggctct
     2941 acgtaagcag cattccatgg ttctggtccg attgcccgta aaaaggtata tgggctcatc
     3001 gtacctgctc ctttttctgt gtcatacgct tgcatcagca tgcagccgtt tgatgaccaa
     3061 aatttttgta atgttaaaat catatcttgg acggtcaatt ttttactcat ctgttttgcc
     3121 cctttcaatt ttaaagcgtt gctgtcaaat aagaatacag gaaagaaaac gacaaaaaat
     3181 ccctatgtct gattacagac atagggacga ataattcgcg gttccaccct acttccgaat
     3241 aaagattcgg cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc
     3301 cggctcccac tctcccgaac tcgctaaacc acgtgttttt tactgatctt ggtcaatgta
     3361 cgtattcatt ttgcagtttt cttgtttcta taatagatcc tctcagtgaa cgatctattt
     3421 gatctacggt taataataga cggaactccc tgtgtttgtc aaatggtttt gattttttgt
     3481 tgtatgtttc atttactatt cttctttttt aggtagatct ttttcagaca tgtctttctc
     3541 tgacgtcttt aatacgtgtg cccaagactt catttgatca atgaactttt tgcttttcaa
     3601 atggataccg acatattcat catacagctg gtcgatgagt tcacggattg cttgtttggt
     3661 ttcttctttc agatggattc cactgatttt atcataagaa atagctgaaa acatccgaac
     3721 gaaataaaca gctcgaggat ctgcatgata acggtgatca tccatcgacc agtgtctttc
     3781 gcatatcaca ccaccatatt tggaagaata gtcaaatttt ccttttgttt ccccacatac
     3841 gacgcagtgt gtccagattg gtgcaattcc gaagcgttgc agtatttgga cttcaaaaat
     3901 attcgtgatg atttctgcat catctccttg gtccatccgt tgcagtgctt gttgcgtaaa
     3961 ctggaacaaa tgcgggtcgt actgatgatc ttcgattgcc gcatctacta gattcaataa
     4021 atatgtgcca tagccgctca aaaagatgtc ttcttggatg tgtcgaaacg cgtggacatc
     4081 tttgacactg cttaaaaatg acaaaccttc acttcgaaaa ttcccgatat aaactgcttc
     4141 agtaaaaggg agcaaagccg gagctaaggg ctgattttgc cgatggatcc ctttgatata
     4201 aaacatcatt ttgccagaag attccgtaaa gattttgact aatttatctt tttctttgta
     4261 attacggcta gataaaatca agccttttga ttctgcaagc tggctcatat tctccctcct
     4321 aaaaaataaa tctggaaaag cagattctgc cgatccagat catttatgtt gcgatcttcc
     4381 gcagcttttc tcaacacgcg gatttttatc cagtttattt ctatacctaa cttacaagaa
     4441 gctcagaata tcaatattcg tcttttcggt agccgaagtc ttgcagatag acttttttgt
     4501 ctcgccagtc tttttgaact ttgacccaaa gttctagaaa gactttgtct cctaaaagat
     4561 tttcgatatc ttttctcgct ttcgttccta cttgcttcag catttttccg cctttgccga
     4621 taatgatccc tttttggcta tctctttcga caataatcgt agcctggata tggattttat
     4681 cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac agaatgaggc acttcgtctc
     4741 gtgttaaaaa caagactttc tcacggatca attcagaaac gatgaagtat tcaggatgat
     4801 cagtcacttg atcatctgga aaatattgtg ggccctctgg catttcactg actaatacat
     4861 ccatcaaacg ttcgacgttg tttccttctg tagcagaaat tggaatcact tgtgcaaacg
     4921 gcatctgcgt tgaataatct tcgatgattc ccaacaactc gtctggatgg attttatcga
     4981 ttttattgat aatcagataa acaggagaat gcacgttttt tagacgttca ataataaaat
     5041 cgtctccccg accgcgtttt tgatcagcgc tgatcataaa taagaccgcg tccacttctt
     5101 tcaacgcact gtaagctgct tctaccataa aatctcctaa acgatgcttt ggtttatgga
     5161 ttcctggtgt gtcaataaaa atcaactgag cttctggtgt agtatatact ccttggattt
     5221 tatttctagt tgtctgcgcc ttatcactca tgatggcaat tttttgaccc acgattcgat
     5281 tcaataatgt ggattttcct acattcgggc ggccgacgat tgcgacaaac cctgatttat
     5341 gttctggcat atacgttctc ctttttaatt aaacaacaaa tgatatattt tagggacaaa
     5401 taaaatcaac ccaacgatga cggaaaaaca agcggtcact aaaactgctc cagctgccat
     5461 atctttgatt tttttcccaa tcggatgaaa atgaaaatcc gtaaacatgt ccacgacatt
     5521 ttcaaaaaca gtattgatga tctctactat ccatacgaga aaaacagcta aaagcagcca
     5581 taaccattct atccgatcaa gctggaaaat cacgcccata aacaaagcga ggattccgaa
     5641 cgcaacatgt ttcctcatgt tgcgttcttc ctcaaaaacg gttttgactc cttggacggc
     5701 aaattccacg gacatcagga aatgtttgtt tttctctacg tttttatctt ttaagtccat
     5761 aggcatctaa gatctctttc tgtaaaccaa acatttcttt ctcatcctct ggtgtcatat
     5821 ggtcataccc gttgatatgc aagaatccat gaaccgctaa aaagccaagt tcccgatcat
     5881 acgaatggcc gtaagcttct gcttgctctg cagcacgttc agtagagatc atgatgtctc
     5941 ccagtacacg aggcaatgga ttctctccat cttcaaaaat cactgggatt tcgtcttcgc
     6001 cctcttcttc caaggcaaaa ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac
     6061 gattgattac ttggatcgct gcattgtcca tgaaagtaac agacatttct gtttcttctg
     6121 gcaactctaa atatgatgct gcaaattgca gcaagtcgtc gatttctttc attttctctt
     6181 ctgagacttg gtttgtttca tccatgaaag taatatccat tatacatcgc ttctttctat
     6241 tcttcttgtt cttcttttat cttctcaact tccgatttca ttttaggata tttgatccga
     6301 gaatggaagg tactgcttaa accgctgatc aatgattttt ctactttacg gatttctttt
     6361 aacgtcaatc cactgtcgtc caattgtcca tcggaaatac gctcttcaat aagattatgg
     6421 acaaaacttt cgattttttc gctcgttgga tggtccattg ctcgtactgc cgcttcacaa
     6481 ctgtcagcta tattgacgat tcctgcttca cggctttgag gttttggtcc aggataacgg
     6541 aattgctctt ccgtgacttc cgggttgcgt tcctttgcct taacgtagaa ataacgcatc
     6601 agcgtggttc catgatgctg tctgcaaata tcgatcacca tttgcggcat attataatct
     6661 tccaatattt tggcaccatc tgtcacatgt ccaaagataa tatgtttact gtcttctggc
     6721 aataagaaat tgtgcggatt ttccgctcct gaaggcaagt tctcaacgaa aaagctggcg
     6781 tgtttgatct tgccgatatc atgataatag caagctactc gagttagtaa agaacgtcca
     6841 ccgatttctg ctacagcatt agcacttaga ttggctacca tcatgctatg gtgatacgtg
     6901 cctggtgctt cttccagcaa ctgtttcaat aaaggatgat taggattgct gagttcattt
     6961 aatacgatca cactatcatc attgaccatc aattcaatat aggggtgcag acctatcgtc
     7021 agcaagaatg agaacagtgc acctgtgaat ccgcatacta gcgttaacca agtcgttgca
     7081 tccccaaagc tcattccttg gtagataatc aaaatgacat ccatcatcac aggaaaaata
     7141 atgacccaca tagcagcgga taacccttgt tctgagatcc gtttgcgtct gacgatcgtt
     7201 gccatgagac cagaaaatac ataggacatc aaaataactg tcagaaagtt cgtgccaatc
     7261 gaatcataaa aaatgaaaag tgcggatact gcttgaaaaa taccagccat gatccctgct
     7321 cttcgattca agaaaaagct taaaacaagt ggagcaaaag ccgcggggta aaacagcgga
     7381 atataagcag cttgttctgt ttggaagagt tggaaaaatt tcattagtac gacgcttact
     7441 gacattgccg tcacgtagaa aagtacgtat tttgttcttt ctcctgcatt ttggaattga
     7501 agtgaattat aaatcagtac accgatttga agcaaaacag ctaaaaccat ggcaatgagt
     7561 gggaaaatag aagtcgtttg attcgtcaat cctagtaatt ccaatttttt catcgctgaa
     7621 gcatcaattt gatttccttc gcgaacaata atttctcctt ggaagatcat cactggttgt
     7681 acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa tacatcatta
     7741 actacaatgc cttgatccac taagtagcga atcatttgtt gctgctctgt tgtgacattc
     7801 aaatattgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct ttcacgaact
     7861 tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaaccgt gtgaatttcc
     7921 gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg gtaaaacgca
     7981 acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg ctcttctgta
     8041 gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgtcatt ataagatttg
     8101 ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg attgttctgg
     8161 tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc tgccagtttt
     8221 cttctttgtt cggtttcttc cgtgttctca atcgttttat tcgcacggat actctcttct
     8281 gctacttggc cttctctgaa atcattggcc ttttgtttga cactactaaa tgtaacgccg
     8341 cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc gagttttttt
     8401 tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa gcttattctt
     8461 gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg tgccgaacaa
     8521 cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttaat gttcgttctg
     8581 catgaacaag accacttgtc gtcccacgag gcaagtctat ctgacttgta tccccattga
     8641 caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga gcgactgtcg
     8701 tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc cgcatgtaag
     8761 ctaaaggtgc gatttcgatc acgcctcgtt ccatcaatcg gttcgtatga tccaatccaa
     8821 aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt tctttcagat
     8881 ccccgggtag aaagcctagg ttttcccctg cctccacagc gggccgcgtc aaaatgattt
     8941 tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga aacgttttcc
     9001 ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat tcaacgtatt
     9061 tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg acgatctctt
     9121 cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccatcttc aatgccgtca
     9181 caacatctgg cgtacttacg tggattcctc gtttgatcaa ttcctgtaat gtccgaatga
     9241 ctgaggcagc caaggaaatc tgggattgct caccaatgat ctgaatgatt tctccacgtg
     9301 tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg tgtgcaccta
     9361 aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta gactcttccg
     9421 tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca tactcagaag
     9481 aaactatata gcaaataaga aaaggttgta aaagagtcgt ttaaccatac gtaccaagaa
     9541 acgttaaaaa atagccgttt ccattcgcta aaggtctcgt tttgataaaa agaagctgag
     9601 aatgtaagtt atatgaaagt ggagaacaaa atattccgtt tcattcagca ggtattctct
     9661 aggtattttt cttgtttcaa aaaaagaaaa caaaatagtt aaaatattct tgcatttcaa
     9721 aaaaaacagg tgtattatta cgtcaacaaa taaacataca gcaaagcgtc gaggaaaaca
     9781 gtaggctatt caacgattta ttcccagtga gtcatgtaag tgagaaatga caataaatcg
     9841 aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca
     9901 ttatcaggct agcggataac tttatcaaaa agcgtccgtg aatgaagaag cttttttaag
     9961 cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga aaagggcttt
    10021 ttttatattt taaggtggtg gttttcatgg aatctgatat tagtggcatt actaattgct
    10081 gcaacgttta tatcgtcaat caaaatgata attattggag gaaaaattta tggaagaaac
    10141 aaaaacttat tcatggaaac aactattgat ggtcagttca cttatcttcg ggatgttctt
    10201 cgggtcaggt aatctaatct ttcctgtcca cttaggtcaa atggctggcg caaactgggt
    10261 cagtgctggc gtaggatttg ccatctcggg cgctcttttc ccattactag ctatcttagc
    10321 tgtagtagtg acaaaaagtg atggtctgta tgacttagct aaacctgtcg gaaaatggta
    10381 tgctgccttg ttcttaatcc ttgttcattt gacgatcgga ccatttttcg gtacaccacg
    10441 gacagctgct actgcttttg aaatggctgc taaaccattt ttgcctgaaa aatataattc
    10501 attaggtatg tttttattct ctgctctttt ctttttactt gcttatttcc ttactgtcca
    10561 tcccacacgg ttggtcaaat acgtcggaaa gtatctaaac tccttgtttc ttgcattgtt
    10621 agcaatcatt tttgtcgttt ctttattcca cccaatggga aatctaaatc aatctgctgc
    10681 agatacttac caaacaaacg ctttaacaaa tggtgtccta gaaggttata acacggttga
    10741 tgcagtagcc ttgcttgcct taagtgtcac ttttgtccat gcagtccgtg accttggttt
    10801 caaagataaa aaagtcacag aattaacagc aaaagctggt agcctgagcg ttgcgctaga
    10861 agtcatgatc tattttggtc ttgttcttct cggtgcgatg agtttgaata agatgtcttt
    10921 atcagaaaat ggcggaactg cactttctca gatcactaat ttttatttag gtaacttcgg
    10981 cgctagcttc ttaggagtga tggtcacatt aggtgtcttc actaccgcta tgggactcgt
    11041 tgtttctttt gcgcaagatt tccataaact gttccctaaa gtcagttata tgacatggct
    11101 gcggttaacg actttcgtct cttttgtcgt tgcaaatgct ggtttagaca atatcatcca
    11161 atggtcttta ccagtattga tgcttttgta tccattgtcg cttgcgttga tcttgctttc
    11221 cttaacagca aaatttttcc aaaagacacc atttgtctat caagtaacga tgttgtttgc
    11281 tgctgttcct gccgtattgg atatgctggc aaattcgcct gctttagtga gtcaacaaag
    11341 agttgtagca agcatgctcg aattttatca tcatcacgtt ccttttgctg cattaggact
    11401 tggctggatg gtcccaacgt tacttggtta cgctggcagc ctactgtttt attacgccta
    11461 tcgcctatca gggtataaac aagaagcaaa tgaactacca gaagaataac caaatcaaac
    11521 acaatttaac ataagaaata ttaaacagga aaaaaccagc tagatgaaag aatcctattc
    11581 tttcgtctag ctattttttt gttctctttt ttacgcgcac aaaaaaactg cggacaatga
    11641 ttgtccgcag tttaaatgat aaaattaaga ttgtaatagt tctttcacaa tggcatttac
    11701 ttggtttcca tctgcttttc ctttgacttt aggcatgatc gcacccatga ctttgccaaa
    11761 ttctttcatt gatgaagcac cagtttgtgt gattgcttct tgaacaagtt gtcgaatttc
    11821 ttcgtcggaa agttgttccg gcatgtattt ttcgactatc acaatttcac tttttacttt
    11881 ttcagctaaa tcgtcacgtc ctgcttcttc aaactcatgc aaagaatcac gtcgctgttt
    11941 catttcgcga gacaacaccg ttaattcttc ttcttctgtc aagtcgtgac cttctttaat
    12001 ctgctcgttt tgtatagatg acttgatcat tcggatgact tgtaaagatt ccttatcttt
    12061 cgctttcatc gctgttttca tgtcatcgtt taatctactt agtagtgaca cactaccaac
    12121 tccaattcat cacagaacta gaattttttg cgttttctag ctgcttcaga ttttttcttg
    12181 cgtcttacac ttggtttttc gtagaattca cgtttacgag attcttgtaa agtacccgct
    12241 tttgacacgg aacgtttgaa gcgacgaaga gcatcgtcaa gtgattcgtt tttgcgaacg
    12301 actgtttttg acatgttaat tccctccctc cgagcttaaa aagtaaccgt aattgttatt
    12361 gaaatatatc tcaaaaaaca agactgtcct gtaaatatta tatctaatgc tgttttgacc
    12421 gtcaaggaaa tcttgctttt ttttaaaatt atcaaatgtt attttattta aatccttatt
    12481 gacaattttc acatcttccg aaaatctcga atctatgccc ttcgatcgta catccaccga
    12541 gctgttcttc gaaataattc attggacaca tatggatttc tttcgttgtt ccgcacaccg
    12601 tacagataaa gtgatggtga tggccgattt ccccttgaca gcaatgaaaa cgaaacttca
    12661 tctctccatt taaatcggtt tcttccagta aatcgatttc tgcaaaatca tggagatttc
    12721 gataaatcgt atcgtaactt actcctttga attcctgatt catatagtca aaaacttctt
    12781 tagcggatac gtagcgattg cgcttcacta aataagttaa caatgcttct ctttttttag
    12841 tatatttaaa accgttattt ttcaatgttt ctagtgattg ttcaatcaat gtctgttttt
    12901 tcatgactta ttcctccaaa tcaaaatgat tacgaagtac acattctaga gtatggtata
    12961 ataaacataa aaaaaatgca agtgaggttt tatccatgtc ttccgattct acagaaatcg
    13021 ttactttttt tgtcatctct gactctgctg gagaaactgc gacaaaatta gcgcaagcca
    13081 ctatggccca atatcctacc gttgagttca atttattccg cagaacattt gtgacagatg
    13141 agaaaacatt gatgcaggca ctgcaagatg cactaaaaga aaaagccctt gtcctgcaca
    13201 cattgatcaa tcaagaattg atcgagatga cgcgttcttt ttgtgaagaa catcaattat
    13261 ttagttttga tgtgatgact ccgccgatat cagaaatcga gcgcttaact ggtatcaagc
    13321 ccattcggca acctggcgcc cttcatttat tgaatgagaa ctatttcaaa cggatcaaag
    13381 caatggaatt tgctgtaaaa tacgatgacg ggaaagatcc tcgtgggttt ctagaagcag
    13441 atgtcgtttt gctaggcgta tccagaactt ccaaaacgcc tttgagcttg tttttagcta
    13501 acaaaaattt aaaggttgcc aatctacctt tgatcccaca agcacatatt cccaaacagc
    13561 tttgggagat cgatcctaaa aaaatcgtgg gactgacgaa taacccagat attttaaaca
    13621 atatccgtaa agaacggatg cgctcttacg gattaaatcc tgatactgct tattccgata
    13681 tcgaaaaaat acgagctgaa ctcgattttg cgaatgatct ttatgaaaaa ttgggctgcg
    13741 tagtaatcaa tgtcgcttct ttatctatcg aagaaacagc ttcacttatt ttgaatgctt
    13801 tagacctaga agatcatagt tattatggaa ctgaaaccac cgaagaaaca gactaacagt
    13861 ttttcttgtc tttttcttaa aaaaactgga ctgtgtcagc gaagaagatt cgttgataca
    13921 gtccagtttg tctctatttg cacctatcgt tattgatgtt ccacatgttt gagcgtctgg
    13981 ttcaaaatat ccagtacgtg agaatcatca agactataca aaatcgcttt tccctcacga
    14041 tgggacttga cgacatggtt ttctcttaat aaacgcagtt gatgcgatac agcagattgt
    14101 tccatttcta ctgcttgggt gattgctgtc acattttgtt ccccgtcttt cagatacagc
    14161 agaattttga gacgagtggg gtcactcaac attttgaata gttggctgac tttctctact
    14221 tttgtttttt gcgtgtctgg caatccattc acctccaact tgaaaaaaga ctgacttcta
    14281 aagtttacca ctttttccat cagtctcaag tttttttatt cttctgtcat ttttttgata
    14341 atcgcttcat aagcggctac tttttcagct gcgtcttctg aagagtttcc ttttacaccg
    14401 atataaaatt tgattttcgg ctcagttcct gaaggacgga tagcgatcca gccattttct
    14461 tccagcgtat acttcaatac gtcagatggc ggtgtcgtta gtttttctac gtttccttca
    14521 cggtctgttc tcgtcaactc tttgaagtct tctgtttgga tgacttttgc tccggcaaat
    14581 tctgtcggtg cttcttcacg gaatttttcc atcaaagcag agatttttgc agaaccttct
    14641 tgtccgctca ttgtcacaga aatcgttttt tctgcgtagt agccatatgt ttcaaaaatc
    14701 gtttgcaagc cgtcatacaa cgttttgcct tgttttttat agtaagctgc tacttctgct
    14761 aataagagca atgcttgaat cgcgtctttg tcgcgaacga aaggtttaac taaataaccg
    14821 taactttctt cgaatccgaa cataaaagta tgggaatgat cttcttcaaa ttgttgaatt
    14881 ttttcagcga tgaatttgaa tcctgtcaat acattgaaca tggtagcccc atagtgttcc
    14941 gcaattgctg taggcaattc gctagaaacg attgatttca ataccgctgc attttcaggt
    15001 aaagtgcctc gcgattggtg tgcttccaaa atatattgag caaacagggc tccgatttgg
    15061 ttccctgtca atacttcata ggagccattt ggcaaacgaa cggctgcgcc taaacggtct
    15121 gcatcaggat ctgttgcaat cagtaagtca gcgccttctt tctcgccaag tcgcatcgca
    15181 tactcgaaag cagagtgttc ttctggattc ggtgatttga ctgtagtgaa atccgggtct
    15241 gcgacagctt gttctggaac tagtacgaat tgttcgaatc cagcttgttt caacgcacgt
    15301 tcgcccaaca tttttcctgt tccgtgaagt ggtgtgtaca caagtttcaa atctttaccc
    15361 attgtttcca ccaattcatg atcaatcgtt actgctttga tttcttctag ataagcggca
    15421 tccacttctt ctccgatgat cgtgatcaat ccgctatgtt cagcttcgtc gtcagataat
    15481 acttcaactt ttaacggatt gctgacttca cggacatatt tcgtcaatgc atctgcgtct
    15541 gcaggaggca tttgtccccc atcttctccg tagactttat atccgttgta agctgctgga
    15601 ttgtgggatg cagttaccat gattcccgca aacgtcttca aatgacggac tgcaaaagaa
    15661 agctctggtg ttggacgtaa actttcgaat acataagaag ggatattgtg atgtgctaat
    15721 gttttggctg cttccatggc aaattctggt gattgatggc gtgagtcata agcgatagcg
    15781 acacctcggc gttttgtttc ttcaccttgt gtatccatga aacgtgcaag tccttctgtt
    15841 gcttgtctta ccgtatagat gttcattcga ttgatcccag gtcctaagat tccccgcatg
    15901 cctgcagtac cgaattccaa cggtgcataa aaagcatctt caagtttttc aggatcttca
    15961 cccagatcaa ttaattgttt ttttaagttt tcttctaaat tttcttcatg tacccattgt
    16021 tcatacactt gttcccaaga catgaaaata acacctcttt ctgaaatatg gttcttaata
    16081 agtatagcat tcacttatta gatatgaaag tacgaggtct aaaaaacgct taaatcaaga
    16141 aaggattctt ttatcaagca cggttgcaag tattcgcata aatcggattc agtagatttc
    16201 acgcgaaaaa aaagagtaaa agaacaattg aaaccgttct tccactcttt ttaaacttat
    16261 gcagttgttt cttctaaaga ttccaagtat ttatacactt gttgtccagc gatcccacca
    16321 tcaccaacag cagtagtgat ttgtcgcaaa tctttttcac gaacatctcc gatggcaaaa
    16381 acgcctggta tattcgtctt catttcgttg tctgttggaa tccagccagc ttcgttcgtc
    16441 aaccctgctt ctctaaatgg tgcagttaat ggatccagtc caacatagat gaatactccg
    16501 tctgctgcca aatcaaactc attgccggtt ttgacattac gtgctttcac acctgtcact
    16561 accatttcat tcccaacgat ttcgtcaacg actgtatccc acataaagga aattttttca
    16621 ttcgcaaatg cacggtcttg gatgatcttt tgtgcacgta attcatctct acggtggacg
    16681 atgactactt cagaagcaaa ttgtgtcaaa tagatggctt cttctactgc tgagtctcct
    16741 cctccgatca ctactagacg tttgttacgg aaaaacgcac catcacaaac tgcacagtaa
    16801 gaaacgccac gtccagcaaa ttcttcctcg ccaggtacgc ctaatttacg gtggatacag
    16861 cctgttgcga taatgactgt ttttccttcg taagttttat cttcacaaat aacttctttg
    16921 tggctgccgt gatccttgat atcttgcacg attccataag cattttctgt cccaaatctt
    16981 gaaacacctt catacatctt ataagctaga tcaggaccca tgattgaatc gaaaccagga
    17041 tagttttcga tttctgccgt attgttcatt tggccgccag gggcgccttg ttcgatcatc
    17101 aatacggata agtttgaacg tgaagcatat aaagcagcag tcatcccagc aggacctgcg
    17161 ccaataacga ttacatcata catatgtaga ttctccttta cttaatcaag taagcatact
    17221 ttacagccaa tgcgaaaaaa agtctattgt tctgcttgcg tttctcgaat agctttatta
    17281 tactcgaaaa atccgctgaa aaacaataga cttttacaaa tcattgatca atcattcgaa
    17341 agattttctc catttgaatc aattactttt ttataccagt tgaatgattt cttcttcgaa
    17401 cgggctaatg tgccatttcc ttcgttgtca cgatcgacat aaatgaatcc gtaccttttc
    17461 ttcatctcac ctgtaccagc tgatactaaa tcaatacatc cccaagtcgt atagcctaat
    17521 agatcgactc catctaattc tactgtatct ttcattgcct ggatatgtgc tgccagatag
    17581 tcgattcgat agtcatcttc cacataacca ttctcatcgg gcgtatcgac tgcacctaag
    17641 ccgttttcta caataaatag tggtttttga taacgatcat aaatatcatt catcgttatt
    17701 ctcaagccta atggatcaat ttgccaaccc cattcacttg cttccagata tggatttttg
    17761 acagaagcaa agatatttcc tgcagtctgt tccaagagtt cgttgtcagt agtcgaaact
    17821 cgagaagaat aataagaaaa ggagataaaa tcaacagtat gttctttcag caatgcggca
    17881 tccccaggtt ccattttgat ctgaatgcca tttcgttcca tttctttcaa tgcataagcg
    17941 ggatattctc ctcgtgactg gacatcaatg aagaaataat tttctcgatc tgattttcta
    18001 gaagcccaaa catcttcagg attgcaagtg tatggataat atgctcctgc tgccagcata
    18061 cagcctactt tgttctccgg atctacttca tgagcaatct tcgttgcgat ggcacttgca
    18121 accaactcat gatgtgccgc ttgatatttg atttgttctt cattttctcc ttcttcaaaa
    18181 tacagccctg cgcccatgaa tggtgcatga aggatcatat tgatctcatt gaatgtcaac
    18241 caatatttga caagtccttt atagcgattg aagatgactc ggcacagatt ttcataaaaa
    18301 ccaacgagtt ctctagaacg ccacgcacca tattctttga tcaaatgcat cggacaatca
    18361 aaatgtgtaa tcgtcacaag cggctcgatc ccgtactttt gacattcttt aaagacatcc
    18421 tcataaaatt tcaaaccaga ttcatttggc tcattttcat cgcccattgg aaaaatacga
    18481 ctccaagcaa tggacatccg ataagtcttg aaccccattt ctgcaaacaa agcaatatct
    18541 tccttataat gatgatacat atcgatcgca atttttgctg gataaaaatg atctttatca
    18601 aattcaaaca ttttcttttt tccagtaatg acaggaaaac ggtcttctcc gattggcaca
    18661 acatctacgt tcgctaaccc acgtccgcct tcatcataac caccctcgca ttgattggct
    18721 gctgtagcac cgccccataa aaaatctttt ctgaatccca tcatacttcc tccattacat
    18781 gtgtattgct tgacaattct cttgccagtt catcttggac ttcgattcct tctgctttta
    18841 accggctgat tgtttccttg aattgcggta aatactgctg atgtgcaata aacaactcat
    18901 ccatgatccg ttttgctgtc tttcctgatc gtataagcgg attgatcgta aacgcttgta
    18961 aagctgttcc ataatcacca gtcacggctg cttcgatcgt cagcaattcc atattcttca
    19021 ttacttgcaa ccagcctctt tcagcagcag gaagggaacc aaacgctaca tttcgtgcgc
    19081 cttgtgcgcc aacataggca gtgacttcta ctacacaatc tgccggcaga tcaggaacag
    19141 ctccatcatt ctttgtagac accacgatct gtgtttcttt atttccatag atcgaagcaa
    19201 tcgtttcaca ggcagcatct gaataataag caccacctcg tttttgcaat tgttcaggtt
    19261 tatgatccaa ttctggatct ttgtataatt caaacagctc tgcttctgtt tctttcactt
    19321 gttgcgctct tgtaccaatc gtttgatatt cttctaaagc atgttccaac atttcttctt
    19381 cacggtagta atagcggtgg tagccacaag ggatcatgtt catttgtttt aattgttctt
    19441 tgaaaaacgg gatgtcaaag atatttttcg gcaatccatt gtcttcatcg tataaacgat
    19501 caatcaaatc tgctgtgcgg tcatttcctt tattatctgc tactttgtgc caatggaagt
    19561 gatttagacc ggcaaacttg tagatcagct cttctttttt cttgccaagc attgctggtt
    19621 ctgtcatcgt cgccattact ggcacattgc ataatccgat cactttatcc catttgccat
    19681 atctgataac tgcttctgtc accatcccgc ttgggttcgc gaaattgatc agccaagcat
    19741 ttggacataa acgcttcatg tcttcaacga ttgccaaaac cactggaacg gtacgaaaag
    19801 ctttgaatat cccgccagca ccatttgttt cttgcccaat catcccataa taactaggga
    19861 tccgctcgtc tttgattcgt gcatctaatt gaccgacacg aaactgagtc gtaacaaaat
    19921 ccgcatcttt caatgcttct tcccgatcca aagttgcata gatcttgact tcatacggag
    19981 aagcatccca catacgctga gacatctgac tgacgatctc aagcttttct tttccttctt
    20041 ctacatctac gagccagatt tctcgtatcg gtaattcctc atatcttttg ataaatcctt
    20101 ccatcaactc gggggtgtaa ctgctgcctc caccaatcgt agcgattttg attccttttg
    20161 ccatgttctc actcctttgc tttttacact tttattatct tttttgtaaa caaaacaaac
    20221 aatagaaatt cgtttctttc cagtaacatt tacaaaagtt agtgttttct actggatttt
    20281 tgtaaatatc ttgtcaaaaa atgactttat taagctatat aacataaatc cgtttacaaa
    20341 tcattttcaa aaaagttata atttacgtaa aaaaactaga agaaggtatc tacatgttat
    20401 tacgtgaaaa aatcaaagaa atctcctttt ctccttcaga aaatgaagtg attcagtttc
    20461 tcttaagcga ttctccagca cttggcgaaa tgaccatcca agagattgct gagcgatgtt
    20521 atgtccaccc gtccacactc atccgtgtag caaaaaaaat gggttttgat ggctgggtca
    20581 gttttcgtaa tgctttttta gctgaaacag cctatcttga agggaatttt caagacgtag
    20641 atgcgaatct tcctttttcc accacagacg gtattatgac gatcgcaaat aaaatggctt
    20701 ctttagaaca gatgacgatt caagatacac tttctctcat tcatcatgac caactgcaaa
    20761 aagccaaaca actaatgctt caagcaaaaa aaatcgtgat tttcgccagt aactcgaata
    20821 ctttgatttc tcaagatttc atgctgaaaa tgaagcgcat caaaatggat gtcacgattg
    20881 tcaccacttt tggtgaatcg ttctatgaag cgtacaattg cacgagtgat atgtgcggga
    20941 ttctgatttc ttatacgggt gaaaacaatg tgatccttcg taccgcagaa atattgaaac
    21001 aaaatcatgt tccaatttta gcgttgacca gtatcggtga ttcaacgcta tcctcaatga
    21061 gtacagccgc tttgcgactt acgacacgcg aacgactgta ttcaaaaatt ggtaatttta
    21121 cgatcaatac atcgatttgt tatcttctag acgtgttata tagttgtgtt tttgcagaaa
    21181 attatcaaca gaatttagtc catctaattt ctgtaggaca aaaatttgat aaacgcccta
    21241 tcagctcagc catcatgaaa gagcctttca attttgaaaa taaagaagtg gactaaaaag
    21301 aatagttaag tctattccat tctttcacct aaccgttcag gtcagtgcgg tgataagtgc
    21361 atcgtctggc ccgaccgtct ccccttgatt ttccaaaata tccaaataat ccatgtaatt
    21421 ggtgacgatt acaggtgttt ctaaactgta tccttccttt tcgattttct ttttgtcgaa
    21481 gcggagcaac agatcgcctt gtttcactct ttctccttgc tgcaccaagc tttcaaaata
    21541 tttcccttcc aactgaacag tatccaatcc gacatgaatc agcaattcca gacctgtttc
    21601 agaaaccatt ccgattgcat gtttcgtggg gaacagggtc atgactgttc cgtcaaaagg
    21661 ggcacgtact tctccttttt cagggtagat caagattcct cgacctagtg ttccttgtgc
    21721 aaaagctgca tcctttgctg ctttcagtgg agcgatttgt ccttgtatcg gtgtttttac
    21781 aatttcttta cctgatcttt tctgtctgtc atctgtttca cctgcttcct ccttccaaaa
    21841 gaaaaaagtc agcgcaaagg ataccaccat cgcaatggcg atcccaatca atacattaat
    21901 cacatctgtt aaatcaccat ctggtctaat atagttggga attgtaaaga tgcctagtcc
    21961 accttgattc caagaagtta aattcattaa cccactatac gcgcccccta acgcaccacc
    22021 gacacaagta aaaataaata tttttttacg tggtaatgta aacccataaa tagctggctc
    22081 tgtaatcccc acaataccag aaacaatggc tggtattgca attgctttgt cttttggatt
    22141 tctaagctta aagtagagtg cggtaagtgc tgcagtttgt ccaaaagaca ctactgcaac
    22201 aggtgtcata atattttgcc aaccattcac agaaaattgc atcaatccca ttggtacgat
    22261 tgcccaatgg agaccaaaca tcaccagaat ttgccagaag aagcctacga ttgctccata
    22321 aataactggc gaaagtgcta aaagtgtttc aaatccagcc attaaaatat cagttgcgaa
    22381 tgtgaatact ggtccaacga gtaaaaaacc taaaggcatt gtaataagaa cggtgaagaa
    22441 tggaacgaag aaattcgcga tcatctcagg tactaacttt ttaattggtt tctgaatttg
    22501 gctagctaac aacacaataa agataatggg aatgacagaa gaagcgtagt tacgcgcaat
    22561 ccagggaatg ccgaatactg tttgataaat aggtgcttca aaaatagtgc cagaaaatat
    22621 ggtcgttagt ggtgcttctg ctgcaccact caaagcttcc agcgacaagc taggattcat
    22681 taatgctgcg ccaagcaaca tccctacgaa ttcatttact ttaaatttac gggcagctgt
    22741 taaaccgata ataacaggca taaattggaa tacagaatca ccgatattat caaacataac
    22801 aaatgtgcca gaaccgcggt caaacgcgcc taagaagaca aggaatgctg tcagtccacg
    22861 aatcatcccg ccagcagcta agatagcaag gaatggttgg aaacagcccg atattccatc
    22921 aataagcata tcaaacggtc tcattttttc tgttgaaaca gcctcatcaa aagtaaggtt
    22981 tcctgctttt ataacctctt caaacacgac tggcacatga ttcccgatga caacttgata
    23041 ctgccctccc gctcgcatta ccgttacaat accgtccata tttttcaaga tctcatcatt
    23101 tgctttttct tcgtttttta atttgaaacg aagccttgtg atacaatgtg tcaaaccatt
    23161 gatattttcc ttcccaccga cattttctac gatttttctc gctaattctt ggtattttcc
    23221 catttctatt ccctccaata tattagtgga aggtttagcc aactgaatgt cactaccctt
    23281 atttcatacg ttcttgttaa tttatcctcc tttttttatt tatttacaac gattctcatg
    23341 cactaaacgc gcagtatgga tcgttaaata cattttttcg tccgtggaca tctggtagtt
    23401 gaacgtctcg gctaaaaagt ctgctatttt ctcaacgcaa ccaaacgtat ccttatattt
    23461 tttttgaata acctgaaaca attcctcaga aacttctccc acttctgatt tttctccatc
    23521 taggacgcgt gtcagaaaaa aacgtaagtg tgtgacgaat cgaaaaaaat ggactgaatc
    23581 ttcatcaaat gtaatcttat agtaatagcg gacgatattt agaatatcct gtattgttat
    23641 agtaaacagg taggtattgt tttgttggct ttgaatgcct tgtgcattga ctaaatgcaa
    23701 tgcgataaat ccggcttcat cttctgataa cttgatgtgg tatttatccg agattttctt
    23761 cactgttttt ttcccgatgg catactcttt aggaaatagt tgtttgatat catacagcaa
    23821 tacatttggt acaaatattc ccattttgtg acgttcgata gccgtataca aatgatccgt
    23881 caatgcaata tacacagaat cattcaactg gacttgtaga gcctcttttg tatattcaat
    23941 gatttcacaa gaaatttcta agtactcaag aggaatcgct tgtgccaatt cttggaactg
    24001 ttgtgaaagc ccgtcatttg tcaactgaaa ttttttgtcg atgcatgagt caaaaatcgg
    24061 gtcacccact ttctttttga atgccaaacc ttttcccatc acgatcattt cctgatttct
    24121 ttcatccctg gttattacga cgttgttatt cagtactttc tcaatcagca tgttttgctc
    24181 ctttccatga tatatagggc tgtattcaaa acaaaaagaa aaacccaaaa agccgtccat
    24241 ggaaccatgg gagactcatc aggtttagct tgcatcaggc aatcactatc caagttgtat
    24301 cgttttcttt atattatcga aagcaaacgc ttttgtcaac gctttcttag tggctattag
    24361 gcacttttga ctagctgtcg tcgtcttttt taaatcgggt gactacgcca ctaacgctca
    24421 cagatgaaga atctgggaag ctctgtttta ttttcactct agcgcttagg acaaaatgct
    24481 actatgctac tgtcacaacc tctttttaca caccactgct ttgcagtttg tacatatctg
    24541 catacaaacc attttgcgct agaagtgttt catgattccc acgctctact atatgacctt
    24601 tgtctaaaac taaaatcaat tctgcatccc gaatcgtgga cagccgatgg gcaatcgcaa
    24661 tcgttgtccg tccttgtcgc atttttgcca atccttcttg gatcaatcct tcggtttcag
    24721 tatcgatatt tgctgttgct tcatccaata ctagtatttt aggatctgtc acgattgttc
    24781 gcgcaaaagt gatcagctgt cgttgtccac tagaatagct ggcaccgtgt tcgatcactt
    24841 ttgagtgata tccttttggt aattcatcga tgaacttgtt tgcttgaaca aactcagctg
    24901 catggcgaat ctgttcgtct gtgatagctg gattcatcat ccggatattt cctgcaatat
    24961 ctccataaaa caagaaggca tcttgcaaga ccaatcccat tttcttacgt aactcttcca
    25021 ttggatattc tcgaatgtca tgatcatcaa tcaagatttg gccttcataa aattcgtaaa
    25081 accgcatcaa tacgttgatg atcgagcttt ttccgcttcc ggtgtgcccc actaatgcaa
    25141 cggtctcacc cggattagcg acaaaagaga tattgttcaa tacattgtgc tctccatcat
    25201 aagaaaagct tacattgcga aactcgatct tacctgaagt aatcgcttga tctgctccag
    25261 gattttgttg cggtgcatat tcttctgtat ccatgatttt caaaatacga ctaccggcaa
    25321 ccatcccatc tgtaaaggtg ctcaaaaagt ccatcatttg agacattgga ttaaaaaatc
    25381 cctggacata ggtcgtaaat gcatagatca gtccaacttc tacgggagaa tgcaacgcat
    25441 cgatcccaaa caaagtcaac gccaacgcaa tcgccaatgc atagagaaaa ttgatgatcg
    25501 gacttaacag aatcgaattc gttttgatca tcgcatagcg cgtgtctaaa tactcgttgt
    25561 tcacttgttc aaattcttta gctaaacgag cttcttggcg aaactgctgg ataatacgca
    25621 tgccggaaat cgattcattc aatttcgtgt tcagctgact taatttttcc cgcatatttc
    25681 ggtaaatacg agaactgaat ttctggtaat accagatgac gattcctaaa atcggcaaga
    25741 acaccaaagt ccacaaggca atttctccat tgatttgaaa catggcaacg aaagaagaaa
    25801 cgactgcaaa gatcccagtc aataccatca aaaagacata ccaaaattca aatagcgttt
    25861 ctgtgtcatt cgtcactcga gaaacggtcg atcctgccgg tgtctggtca aaataacgca
    25921 tacctagtgt atgcagcttt tcaaataatc ttacccgaac atactgatag gtcttcaatg
    25981 aagccatgga atacaaatac cattggaaaa accaaatgat acttttgact atgactccaa
    26041 acaagtacac accagcaaaa aacaaaataa cttgtgtcgt agctgactgt ttagccagat
    26101 gatcatccat aaaagtttgg atgatccttg gcaataggac attgatcaca gacagggcaa
    26161 acgcaaataa gatagcaacg atgaacgttc ttctaaaagg tttggcgaac ttcatcagtc
    26221 gtttgatgat cttcttctgt tctttaagtg gaatcgattt tgtccattcc gattgataat
    26281 tttcatccat caggcctcgc ctccttctat cttcgcttct aattgctgtt tttcccacat
    26341 tcgttcatac cagccaccta atgcaagcag ctcgtggtgg gttcctcgtt caatgatttt
    26401 cccttgatcg acaacaatga tttcttttgc atgcatcacg ctgcttaaac ggtgagctgc
    26461 aatgatggtt gttttctctt ttcttatttc ttttaaatta tgcaagatag cttcttctgt
    26521 tttggcatct actgcagaaa gtgcatcatc taagatcagc aattcaggat ttttgatcaa
    26581 tgctcgagca atcgaaatcc tttgtttctg tccgccagat aaagagactc cacgttctcc
    26641 aactaaggta tcataacctt ttggcaattc cttgatttca ttgttgataa atgctaaaga
    26701 agccgcatgc tctacttctt cttctgaaag ttttggatta gaaaaacgga tattatctcg
    26761 aatggtcatc gaaaacagga agtgatcctg aggcacataa ccgattgatc ctaataaagc
    26821 attcaatgaa tattctttga tcgaacgtcc gccaaattgg attttccctt gataatcatc
    26881 gtattcacgc atcagtaatt tgatgattgt cgtttttccc gcaccagttt tcccaacaat
    26941 acctaatgtt tctccttctt ggatcgcaaa tttgatttgt tctaatgctg gtgattcttc
    27001 tttcggatag ttaaaagagg aaatagctac attcagctcg ccttttgccg gggtctgcac
    27061 tgcacccagt tgttccacga tatgcgtctt ttcatgcaaa agttcattaa cgcgatcata
    27121 agaagcattt cctctttcca atacgttgaa caatcggcca atcgcaaaca tcggccaaac
    27181 aagcataccg atataactaa taaatgagac catctgccca atcgagatcg tgccttccaa
    27241 gacatacttt ccacctaaaa taattgttgc aacataagac agaccgataa tcaaggtaat
    27301 gaatggatca aataaggagt ctaaaaaatt gactcgtttg tttttaataa ttgcatcttc
    27361 aatcttttct tcaaaatccg cgagatcttc tttttcttgt ccaaatgttt taatgacttt
    27421 gatcccagtg atactttctt gtgtcttatc attgatacta gaaaaagctt cttgcgaatc
    27481 acgaaaagca tcgtggagtt ttgaccccag cactcgtgaa gtaacggcta gcaaaggcag
    27541 aggaatcagc gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg caatgatcgt
    27601 tgttccgccc gtaatcactg aatctgcaaa ggtcaagatt ccagctcctg ctacattctg
    27661 gattgcattc aaatcattcg ttgcatgagc catcaaatct ccagtccgat atttttggta
    27721 aaagagattg tccatttctg taaaatgttt gaatagctgc tgccgaagtt ctttctccaa
    27781 ccgagctgca cttccccaaa tattcgtccg ccagatataa cgaaaaagat attgtaagaa
    27841 agctgcaagg atcaatagta agatccacca agaaatgatc tttattgata aattcttatc
    27901 ggcgatctca tcaatgatga tcccaatcac ttttggagga actaactgta ccaaggccac
    27961 tgcgaatagg gagaaaatcc caattaaata atgtttcttt tcctgacgaa aaaaccaacc
    28021 taattttcta aaaatcgaca ttttactcct cctttgtctt tatcaagagc ataggattgt
    28081 ctgtccttgt cttttttgct gaatagtctg aatagtctaa caaattacga aataaaaaac
    28141 ggactgaaaa aatcagcccg caaaaagtct ttgggtcaga acctattttt tcccttggtt
    28201 cttcatttgc tgcatcatct ggcgaatttt cttctcagat ggtttttgcc ccattgacat
    28261 catcattgat cgcaacatgt cctcattaac gggaggattt ttttctaaat agtctttcat
    28321 atatttacgc gctaggaaaa agccacctac tgcgccgatt agcaaagcaa taatagcaat
    28381 aagtacgaca atacctgttg ataccatata aatctctcct ttctatttcc gaattgctct
    28441 caatgattta ttttactaaa gtttattgaa aaaagaaagg cgtattcttt agtttcatgc
    28501 aaaaaatagt ggttttttta gagaagaaca aaatttcttt cattaaccag caagacacgc
    28561 cacgaaaaaa aattaggcat accgaatctt tttctttaat tttttctttc tgaggtgtag
    28621 aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat ccaacaaaca
    28681 atttcataag catcgactta ttgaacatac aaatggttta tctttagctg aagtaaatgg
    28741 aacagtggat gtaccaaagg gaaaaagttt ttttaaaaca cttcttgctt actctggacc
    28801 aggtgcatta gtagcagttg gttatatgga tccaggtaac tggtccactt cgatcactgg
    28861 tggacaaaat ttccaatatt tattgatgtc agttattttg atgtctagtc taattgctat
    28921 gcttttacag tatatggcag caaaattagg aatcgtgacc caaatggatt tggcccaagc
    28981 aatccgagca cggacaagta agacattggg tgtcatttta tggatcttga ctgaattagc
    29041 cattatggca acagacatcg cagaagtcat tggtgcagca atcgccctct acttactttt
    29101 tgatattcca ttggtaattg ccgtatttat cactgtgtta gatgttttcg tccttttatt
    29161 gctgacaaaa gtcgggtttc gtaaaatcga agctttggtt gtctgcttga ttttcgtcat
    29221 tttattcgtt ttcgtctatc aagttggctt gtctaaccct gattggcaca gtttatttgc
    29281 tggctttatc cctaacagtc atacattctc agactcacct gtcataggtg gtgaaactcc
    29341 gctgactgga gcgttaggga ttattggtgc aacagtcatg ccgcataact tatatcttca
    29401 ttcagcagtt tctcaaactc ggaagatcga tcaccaagac gaagaagatg tagcacaagc
    29461 ggtacgcttt tctacttggg actctaatat ccagttgtca atggcttttt tagtcaatgc
    29521 actgctattg atcatgggtg tcgctgtatt caaaaccggt tctgtccaag atccttcctt
    29581 ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta tcttgattgc
    29641 tgtagcaaaa tctggtatct tgtctacttt atttgctgta gcacttcttg cttctggcca
    29701 gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggat tcatccatat
    29761 gcgtatcccg atttggctaa gaagactagt cacacgattg ctttctgtga ttcctgtatt
    29821 gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg ctttgaataa
    29881 cttgatgaat gaatcccaag tttttctggc ttttgcattg ccattctcga tgattccttt
    29941 actgatgatg accaatagcc aagcggagat ggggagccga ttcaaaaatt catttgttgt
    30001 aaaacttctt ggctggttct ctgtgattag tctgacttac ttgaacttac gaggattacc
    30061 aggtcaaatc gaaagtttct tcggcgagca agccacctca gctcaactag cattggctga
    30121 tcaaatcgcc tatgtaatca ttgttcttgt ccttctcctg ttggcatgga cgattggtga
    30181 tttatataaa ggaaacaaaa aatatgaaca gcgtttgcaa gaaacgatag aagaaatcga
    30241 atagaaactg attcttttac acaaaggagg ttgtgacaga agtgggcagc ttcaagaaat
    30301 aagaaggaat ttaagaaaat tgcttttcaa atttttgtga aatttcggct tatttccgaa
    30361 gaagttactt ctggaacacc gtgtat
//
LOCUS       DAZKCN010000023        30282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000023 DAZKCN010000000
VERSION     DAZKCN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.023"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..1754)
                     /locus_tag="ACG8K3_000975"
     CDS             complement(174..1754)
                     /locus_tag="ACG8K3_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9144873.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2155..3531)
                     /locus_tag="ACG8K3_000976"
     CDS             complement(2155..3531)
                     /locus_tag="ACG8K3_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9144874.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3659..4747)
                     /locus_tag="ACG8K3_000977"
     CDS             complement(3659..4747)
                     /locus_tag="ACG8K3_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9144875.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4919..5353
                     /locus_tag="ACG8K3_000978"
     CDS             4919..5353
                     /locus_tag="ACG8K3_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9144876.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5431..5964)
                     /locus_tag="ACG8K3_000979"
     CDS             complement(5431..5964)
                     /locus_tag="ACG8K3_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP9144877.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="ACG8K3_000980"
     CDS             complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="ACG8K3_000980"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9144878.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7229..8026)
                     /gene="recX"
                     /locus_tag="ACG8K3_000981"
     CDS             complement(7229..8026)
                     /gene="recX"
                     /locus_tag="ACG8K3_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9144879.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8133..9515
                     /gene="rlmD"
                     /locus_tag="ACG8K3_000982"
     CDS             8133..9515
                     /gene="rlmD"
                     /locus_tag="ACG8K3_000982"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9144880.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9689..10267)
                     /locus_tag="ACG8K3_000983"
     CDS             complement(9689..10267)
                     /locus_tag="ACG8K3_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP9144881.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10589..11059
                     /locus_tag="ACG8K3_000984"
     CDS             10589..11059
                     /locus_tag="ACG8K3_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP9144882.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11167..12393)
                     /locus_tag="ACG8K3_000985"
     CDS             complement(11167..12393)
                     /locus_tag="ACG8K3_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9144883.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13147..13347)
                     /locus_tag="ACG8K3_000986"
     CDS             complement(13147..13347)
                     /locus_tag="ACG8K3_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9144884.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13481..14089)
                     /locus_tag="ACG8K3_000987"
     CDS             complement(13481..14089)
                     /locus_tag="ACG8K3_000987"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP9144885.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14174..14644)
                     /locus_tag="ACG8K3_000988"
     CDS             complement(14174..14644)
                     /locus_tag="ACG8K3_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP9144886.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14692..15606)
                     /locus_tag="ACG8K3_000989"
     CDS             complement(14692..15606)
                     /locus_tag="ACG8K3_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9144887.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15752..16555
                     /locus_tag="ACG8K3_000990"
     CDS             15752..16555
                     /locus_tag="ACG8K3_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP9144888.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16733..17680
                     /locus_tag="ACG8K3_000991"
     CDS             16733..17680
                     /locus_tag="ACG8K3_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9144889.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17748..18041)
                     /locus_tag="ACG8K3_000992"
     CDS             complement(17748..18041)
                     /locus_tag="ACG8K3_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP9144890.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18069..18734)
                     /locus_tag="ACG8K3_000993"
     CDS             complement(18069..18734)
                     /locus_tag="ACG8K3_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP9144891.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(18874..19506)
                     /locus_tag="ACG8K3_000994"
     CDS             complement(18874..19506)
                     /locus_tag="ACG8K3_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9144892.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19513..20580)
                     /gene="liaS"
                     /locus_tag="ACG8K3_000995"
     CDS             complement(19513..20580)
                     /gene="liaS"
                     /locus_tag="ACG8K3_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP9144893.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20577..21308)
                     /gene="liaF"
                     /locus_tag="ACG8K3_000996"
     CDS             complement(20577..21308)
                     /gene="liaF"
                     /locus_tag="ACG8K3_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP9144894.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(21476..21955)
                     /gene="greA"
                     /locus_tag="ACG8K3_000997"
     CDS             complement(21476..21955)
                     /gene="greA"
                     /locus_tag="ACG8K3_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9144895.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="ACG8K3_000998"
     CDS             complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="ACG8K3_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP9144896.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            23431..24591
                     /locus_tag="ACG8K3_000999"
     CDS             23431..24591
                     /locus_tag="ACG8K3_000999"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP9144897.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(24631..25578)
                     /locus_tag="ACG8K3_001000"
     CDS             complement(24631..25578)
                     /locus_tag="ACG8K3_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9144898.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(25711..27102)
                     /locus_tag="ACG8K3_001001"
     CDS             complement(25711..27102)
                     /locus_tag="ACG8K3_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9144899.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            27228..27638
                     /gene="tagD"
                     /locus_tag="ACG8K3_001002"
     CDS             27228..27638
                     /gene="tagD"
                     /locus_tag="ACG8K3_001002"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFP9144900.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            28052..28852
                     /locus_tag="ACG8K3_001003"
     CDS             28052..28852
                     /locus_tag="ACG8K3_001003"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9144901.1"
                     /translation="MRLRKQHYHYQSCGKYFTAHTYLVVPSCFISKQVHYTILEELTE
                     RQALKTLTKRCDLSVTTFQRTLSALSAMLRQMLDYLPHCLLFDEFRSLSISHGKFSFS
                     CMNRETGKLFDILSSRRKKDLIAYFMRFTRKAHMGVRYIVTNMNAPYFSLVKESFPNA
                     QIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKDTSQAKYYRQLKPLYKLLLKSDDELDY
                     TTFKKWQNFQWRYLTESEVVDRLPSISYELKIAKQFIKSCLAIITRNS"
     gene            28961..29107
                     /locus_tag="ACG8K3_001004"
     CDS             28961..29107
                     /locus_tag="ACG8K3_001004"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9144902.1"
                     /translation="MTKLENLRTHIKTLKRFAYGFRSFTNMRLWIFLINRLISYALKA
                     SNSY"
     gene            complement(29174..>30282)
                     /locus_tag="ACG8K3_001005"
     CDS             complement(29174..>30282)
                     /locus_tag="ACG8K3_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9144903.1"
                     /translation="VKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLS
                     SVLLIIIFVSTKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPA
                     FIILTPQLFMQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKI
                     SKAQAEGKSENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINML
                     ISCLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATI
                     VLVVVELLVFFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFL
                     DMAIKSIMIFIIYLPFVYFLFPDIRNEIRKITRK"
BASE COUNT         9449 a         6007 c         4805 g        10021 t
ORIGIN      
        1 taactagcac cacgggtatt tttatatttt tttgattaga catttctatg attttaatat
       61 agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agaggctgtt
      121 tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc
      181 aaacttttta attttacatt aggatattta tccgcaaacc agcgttctgc aaattggttc
      241 tcgaataaga acaatggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc
      301 atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac
      361 atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc
      421 tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct
      481 ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt
      541 gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt
      601 agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg
      661 atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca
      721 ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat
      781 gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct
      841 tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct
      901 tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt
      961 acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt
     1021 ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt
     1081 tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat
     1141 tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag
     1201 atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat
     1261 tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg accatcacga
     1321 tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat
     1381 ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg
     1441 gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata
     1501 tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt
     1561 tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg
     1621 gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc
     1681 ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat
     1741 tctggattat tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg
     1801 cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt
     1861 gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc
     1921 acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg
     1981 atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca tggattcatc
     2041 gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa
     2101 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
     2161 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
     2221 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
     2281 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
     2341 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
     2401 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
     2461 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
     2521 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
     2581 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
     2641 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
     2701 accgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
     2761 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
     2821 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
     2881 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
     2941 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
     3001 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
     3061 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
     3121 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
     3181 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
     3241 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
     3301 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
     3361 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
     3421 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
     3481 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
     3541 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
     3601 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
     3661 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
     3721 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
     3781 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
     3841 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
     3901 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
     3961 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
     4021 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
     4081 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
     4141 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
     4201 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
     4261 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
     4321 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
     4381 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
     4441 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
     4501 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
     4561 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
     4621 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
     4681 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
     4741 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
     4801 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
     4861 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
     4921 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
     4981 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
     5041 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
     5101 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
     5161 cagacaccaa gggttaggaa aaatactgat tgaagaagca gaaaactttg ctaagaatca
     5221 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
     5281 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
     5341 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
     5401 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
     5461 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
     5521 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcggataat tcatcatttt
     5581 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
     5641 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
     5701 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
     5761 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
     5821 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
     5881 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
     5941 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
     6001 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
     6061 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
     6121 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
     6181 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
     6241 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
     6301 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
     6361 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     6421 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     6481 tccagaatga ttttgaagag ccgcactgat ataataaacg tcttttggct ttgctttttt
     6541 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     6601 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     6661 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     6721 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     6781 tcgcatcacg ttgccgtcaa ccgcgggttc tggaagtcca aatgcgatac ttgcaatcgc
     6841 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     6901 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     6961 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     7021 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     7081 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     7141 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     7201 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     7261 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     7321 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     7381 aataatcata ttctatttct tgatctgctt cttgcggaag atcagccatc gcttgttgga
     7441 tcacttcttg agaaaaacct ttcgtcatca atgtttgttg gatttttctc aataattctt
     7501 tttggctttt tccgcgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     7561 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     7621 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     7681 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     7741 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     7801 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     7861 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     7921 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     7981 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     8041 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     8101 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     8161 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     8221 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     8281 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     8341 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     8401 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     8461 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     8521 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     8581 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     8641 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     8701 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     8761 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     8821 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     8881 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     8941 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     9001 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     9061 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     9121 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     9181 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     9241 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     9301 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
     9361 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     9421 aaaagttcaa gattgaatat ttacagtctg tcgacatgtt tcctcagact gcacgttgtg
     9481 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     9541 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatacc
     9601 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     9661 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     9721 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     9781 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     9841 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     9901 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     9961 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
    10021 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
    10081 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
    10141 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
    10201 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
    10261 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
    10321 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
    10381 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaac caaatcatat
    10441 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatc
    10501 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
    10561 actactatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
    10621 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
    10681 agaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
    10741 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
    10801 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
    10861 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
    10921 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctacatc atcgcctggc
    10981 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
    11041 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
    11101 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
    11161 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
    11221 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
    11281 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
    11341 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
    11401 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
    11461 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
    11521 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
    11581 ctggtcttgt ttgattgttt caaacgcagg ggtgtagttc gtcgctacat caagcatgtc
    11641 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
    11701 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
    11761 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
    11821 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
    11881 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
    11941 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
    12001 catttcgaca ccaccaaatg cataggcatg atttaattta tctaccgttc cataacctac
    12061 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    12121 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    12181 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    12241 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    12301 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacaa atacccctaa
    12361 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    12421 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    12481 aaaaaacatt tttgtgtaac gattactatt ttttaaaatt tctcaaaatt cttaactatc
    12541 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    12601 ttttttacgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    12661 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    12721 atcattcgat ttcacgaaaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    12781 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgatttcat
    12841 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    12901 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    12961 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    13021 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
    13081 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
    13141 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
    13201 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
    13261 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
    13321 gaaccattta accgttccgt gttccataaa tgtatagcct ccaaaaaagt tcttgcaagt
    13381 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
    13441 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
    13501 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
    13561 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
    13621 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
    13681 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
    13741 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
    13801 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
    13861 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
    13921 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgca tttaaattcg
    13981 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
    14041 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
    14101 tcctcaatgt tttctttata atgtctacat tacaaagagt aacaaagaga aggaaattct
    14161 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
    14221 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc taggataaca ataatttgag
    14281 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
    14341 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
    14401 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
    14461 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
    14521 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
    14581 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
    14641 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
    14701 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
    14761 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
    14821 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
    14881 ttcgctatac tgccaaatat ggacatgatg aacattcttt accccatcga ttgcttcaat
    14941 ttcttgttcc attttttcat agtcaatagc aggtgcactt tgtatcaaaa tagcggcagc
    15001 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    15061 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    15121 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    15181 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    15241 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    15301 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    15361 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    15421 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    15481 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    15541 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    15601 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    15661 caatcattca gatgtttccc taaagcactt gtatttcact tgatacttcg ctagaattgc
    15721 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    15781 ctttatacga ttcaaagacc taaaggagac gagaaatgtc ccattttgga aagccattgt
    15841 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    15901 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    15961 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    16021 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    16081 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    16141 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    16201 tttaaaaaac ttaacaggta aggagctccg ttctttttta agtaaagcat ctataccact
    16261 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    16321 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    16381 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    16441 aacaatcttg atggtctttc ttccttcttt tgacacaagc gccttcattg cttttgcagg
    16501 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    16561 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
    16621 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    16681 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    16741 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    16801 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    16861 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    16921 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    16981 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    17041 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaagcacaga gtgacatgaa
    17101 atggttaggt tcatcggtcc attaccaatc tatccaagga aaaaaaatac taatcattgg
    17161 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    17221 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    17281 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    17341 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    17401 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    17461 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    17521 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    17581 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    17641 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    17701 aaaagaacaa agagaaggtg acaaaacttg tgtcaccctc tcttttttta ttgtttttta
    17761 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
    17821 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt
    17881 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
    17941 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
    18001 tcaaaccact tttgtgcttt tggcgttacc gttaattcca tcccaacaac ctactttctc
    18061 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaatc
    18121 accagtagat tatcattttc gcgaacatat tcatctgctg caggagaagc gataaccgtt
    18181 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
    18241 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
    18301 tctgacagct ctaagtagtc tagaatattc tttgatacta agttatgtgc aatccgtatc
    18361 cccatgtctc gctcagggtg tacgacacga tctgctccga tcttctctag tacacgggca
    18421 tggtactcat tttgggcttt tgctaaaata ttcggtacgc ccatttcctt taccatcaga
    18481 gtaactaaga tactggcttg gatatcttct ccaatagcga caattacatg atcgaaattt
    18541 cttataccta atgaacgaag tgtcatctca tcctgtgcat ttgccacaac agcatgagtg
    18601 gctatattca tatattcatt gactcgatct tcattactgt caatagccag tacttcttgt
    18661 ccagcttcga tcagtgtacg gcagatgctt ccgccaaatc gtccaaggcc tacaattgca
    18721 aaattttgtt tcaccatttt cctcctttag tatcttcttt tctcagtata acaaagcctt
    18781 tccaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
    18841 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttaaacgc
    18901 atagatagct gcttgagttc ggtcttctac ctctaactta gctaatatat tcgacacatg
    18961 cgttttaact gttttcagtg tgataaacaa ttcatccgcg atttcctgat tacttttccc
    19021 ttcagaaatc agcatcaaaa tttcattttc tcgattcgtt aattcttcat gcaatactgg
    19081 atcatttcgc cgactcattt tttccatcat ttttgttgtc acttcaggtt ccaatactcg
    19141 ctcgccgcgt tgcgtagccc ttatagcatc agctatttca tgtgcggtag acgtcttcaa
    19201 caaatatcct gctgctcctg cttcaattgc aggataaact ttttcatcat caataaaact
    19261 tgtgacgata atgattttgg cttttggaca atcttttaag attgcttttg ttgattcgat
    19321 gccatccatt tcttccatga ctagatccat taaaatgaca tctggtcgaa gtgccatcgc
    19381 cttttcatag ccttgacgtc cattttcagc ttctccaatc acttcaatgt cttcttggat
    19441 agataaataa gaagagacgc ctaagcgtac catttcatgg tcatctacta ataaaacttt
    19501 tatcatcttt atctatgcct ccttcatcaa agggaccttg atctccacgc tcgtaccttg
    19561 ccctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
    19621 gttcaatcca taacttcccg ttttggtttc attcatatta aatcctgtcc cgtcgtcaat
    19681 gatacgtaat aaaagattgt tatctatttt atgcaaatag acttctaatt cattagcctt
    19741 agcatgtctt aatgtgtttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
    19801 ggaactagtt aattttacat cttctacatc ccattttagc gaaatctgga ttttattctg
    19861 caattccttc aataactgct caatgccttg ttttaaactt ttttcttcta aattgactgg
    19921 acgcagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgccac
    19981 cattttcaac tgcttacgga acatttctgg tgtctcactt ttttctgctt gctccgtcaa
    20041 tgccgacatc atcatcatcg ctgcaaataa ttgctgtgaa actgaatcat gaagctcacg
    20101 agctaatcga tgcctttcta attctaaaat ctcttctttg gtttgtccat ctacatattg
    20161 aggacggcta gtcataatct gtaattcact ggatacttcg atcatttttt cttttatttt
    20221 agcaatttcc atatcaatat ctttgatgta cagatcatcc gacgcgcttg ggatcggctg
    20281 atgcaaaagt ttggattcat aattgccaga agaaagtgca cgaagctttt cttcgatttt
    20341 cccgtatttt gccttttgaa taagtgataa caatagaaat acgatcaatc ctacacctaa
    20401 tgaaattgcc atcaaatgga agataagcgg aacgtataat aaacgcgcac gcagcaattc
    20461 tccccaccaa tgactttggt tactggcata aaaataagtg aaaagtgaaa atagaataat
    20521 aaggaaagcc gcaatccccg aataaacagc tagcattgct ttggatattc ttcccatcat
    20581 attcgtatca cctcgacatc tccaaccaag gtattggtaa tgatcttcaa tctgcgagga
    20641 ttttcatcat aatcactgct gcagattttg atctgttcgt tttttaagga aaactgctct
    20701 tcttcaaaca agacatttcc tactaatgtc gcatgctcta aacgaatagc cactcccagg
    20761 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
    20821 ggcaaaagtg tattacccaa atcgatgata gtatcaccag aaatgatggc aatgttgata
    20881 tcatcccatt catagacgtc gtttccaata cgttggttac caaataattg ctgtctttta
    20941 cgctcattat tatgtgtttt taattgctct gtttggacca tcatgatctg tttttttcgc
    21001 caaaaagcat tttttgtcaa atctattcct gaaatctcta cacctttcag cccgataaat
    21061 aaaacggcca atacaaccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
    21121 accaagctcc ctaagatcaa ttgaaaattt tgaaatttag tacgaggata tttgcgcata
    21181 gctagatata tattgaaaat accgaatatt actaaaagca aaagtccagt attgtttacg
    21241 atttgccaaa cagcaaataa cagcagcagt gcttcgacta ccacaaaaaa acgccaagaa
    21301 ctattcatcg ctttttctcc ctcctgacac attctcattt catcatacct atttttttga
    21361 ctgttggcta taggacttta gatgtaagat cgttcagtga aacctgcaat actcaaagag
    21421 aaaagatcta gagtgcgtca tcagtaagat gaactctcta gatctttcag tcagactacg
    21481 cttgttctac tttgacgatt ttaactaaca tatcgccacc tggtgtagca atggttactt
    21541 catcattttt acgtttgcca atcaatgctt gcgcgatagg agaatcattg gaaatctttc
    21601 cagaaaacgg gtctgcttca gcacttccga caatcgtgta ctcttcttca tctccatcag
    21661 gcaactcgat gaacgtaact gttttaccga tcgatacctc atctgcatct acgccgtcat
    21721 tatcgatgat ttgtgcaaaa cggatcatat tttcaattgt tgtgattctt ccttcaacaa
    21781 atgcttgttc atctttagct gattcgtatt cggagttttc ggaaagatct ccgaaaccgc
    21841 gagcgatttt gattcgttca atgatttctc cgcgtttaac tgtttttagt tcttctaatt
    21901 ctttttctaa tttttcttta ccttctagag tcattggata aactttctct accatgtgta
    21961 acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat atagtccaaa
    22021 aaagattacc acgcttttct taaaatgtaa atgtttttct aaaaatgatc aatgactagc
    22081 ccctgttttt ctaagaatta tttacatatt tttgcactag ttccatatgt tcttcatacg
    22141 ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa tagtcatttt
    22201 ctgctggttc taatactgcc ttgattgcct tttcgcttgg attgtcaaaa ggaccaggcc
    22261 catagccggt attggtatac aaattgtatg gtgaatctac agctgtatct tcatacgtca
    22321 ccaattcttt atgttctcct aatgcatata aaacagagat atctgactga agtggcatat
    22381 ttgccttgat tcggttaaag aagacttgtg caatattttt acgatcattt tcttttacgc
    22441 cttctttttc aactagagaa gccaaagtta aaacttcttg tacagtcaaa tttttctgtt
    22501 tgatagctga ataatagttt tgcatgaccg tatttgtctt gttcaccatc tcaatcacaa
    22561 gatccttaag cgaagtactc gtgtagtaat cataagtagc tgggaataag tagccttcca
    22621 aacggtattt tgtgttctca gctttagagg cactttccaa caattctggg aatttttgat
    22681 acaactcgtt aaagaaagtc tcatcattta ctaaatctaa aaattctttc ttgtctttac
    22741 ctgttacctt cgctacccgc tcagcaatct gatcgatgtc gtagccttct ggaatggcaa
    22801 ttttcccatc tgcaactttt gttggctcag atgtgccgcc ttcttgcaac tgcttgccaa
    22861 tctcatcaag agtcatatta ggcgctaact gataataacc agcttgaaag ccagttaagt
    22921 ttttaaattt tgtataataa ttgaaaacaa tcccactttt aataatattg tctttctcca
    22981 aaatttcgcc aatctgctta ttagaagagc cgtttgggat ttccacttgg actaattggt
    23041 cgtccgattt atctaaaggc ttcaacccgc tatctacata acgatatacg gtaaacccga
    23101 gaaatccgcc aataatcaat aatgtaagag cgatcactaa aatgatcttc ctcacgatct
    23161 tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta gctgccttac
    23221 tggcttcttt tttgtctttg cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt
    23281 actatcatct tcgtaactct gtttattata catgaaattt ctactgccgt gaattatttt
    23341 accaaatatt atgtcaaaat tgtaagtggc aaatgctctt tttgaggatt acaatgaaaa
    23401 atagttaaaa atggatagaa aggatttaat atggcaaaag gaaaacatca aaaaaaacag
    23461 cagatctctc tttcttttct cgcagttttg ttgctggctg tcagcttctt ttcaacaggc
    23521 atcattacag gatggcaatt aacaaaacaa cctcaaccac aaatttataa aacggaaccc
    23581 gcacaacttg cttccaattt aacatctaat gagcagatgc ttgccagcaa aattgatcaa
    23641 ttactgcaaa aatcagatta tatcggttcg atttatgtac gcaaaaataa tcgagtgata
    23701 ttagaaaaag gatatgggta cgcaaatcta cgcataaaag aatcgaattc tccttctctt
    23761 tactatcaaa tcggttcgat ccaaaaagcg atgactgctt tacttatcct aaaacaaatt
    23821 caatctggca agataacttt tgacacaaag ctatctgaat tttatcctca gattcctgga
    23881 agtcagcaaa tctcaatcaa aaatatgctt tacatgcgct ctggtcttca tcgaaccgct
    23941 tctccaaaaa aacctatgtc agatagtgag atcattcagt ttgcccttca tcatttgaaa
    24001 tttactggtt atgatacgta tcattatgaa ccattaaatt atacattgct tacaggggta
    24061 ttgatcaaac taatgaatca gccttatgaa actcttttaa aaaaagaaat cattcggcca
    24121 ctccatctag aacatacaga tttttatgaa aatgtaaaaa attcccctca gcatgctgta
    24181 tcttaccaaa tgtctgcaac tgacgattat tcaaaagcat tggtcgagcc tgaaacagac
    24241 atccgaaatg aattaggcac aggaaatata agtatgtctg tttatgactt gaacaaattc
    24301 tttaccagtg ttctgtctgg cgatattata ccaaaagaac tacttttctc attgtggcaa
    24361 aataatggtg ataaacatcc ttacagcggc ggtgtttaca gtggaaatga ttatatcctt
    24421 gctcagggaa atatcaatcg attccatgca gtagcggcga tgaaaaaaga cacaaaagat
    24481 gcaatcgtca tggaaagtaa tatccaatct gataaaaaca tcaaattgcc ggccactgat
    24541 ttaagaaatc agatttacga actgatggaa ggtgttaatt taaaaagcta agtcgagaca
    24601 aaaaaaccag caggatgctg gttttttgtt tcaatcgatt tctgtcttta aataacgttc
    24661 cattccgttc aatatatcaa atttgggttg ataacctaaa gaacggatct ttgaaatatc
    24721 cgctaaagaa tcacgaatat cccctgaacg ctccgcttca tatttaagcg ctagctctgt
    24781 tcctaagatt tgatcaattg aatgaatcaa ttcaagtagc gtagtgcttt ttcctgtacc
    24841 gacattgaat tgctgtccta aagctttttc ttccttagca acaagtaaaa gtgcttgtat
    24901 aacatcttct atataaacga agtctctaga ttggctacca tcaccaaata aagtaaattc
    24961 tgttttttct cctgccaatt gttttttgta tcggtccact agaatcgaaa taactccgct
    25021 atatggagag ttaggatttt gatttggacc ataaacgtta aaaaatcgga cagcactagt
    25081 cggaacatca tataaatgac agtaatccaa aacatattgt tctgatgcaa acttatcgat
    25141 ggcatatggt gttaaaggac ggatgaccga ttcttctttt tttggcaacg ttggttcatc
    25201 gccataaact gcagctgatg aactaaatac caatcgtttt aaaccagatt gatattttcg
    25261 taccaattca agcaataaaa gcgcactgtc aaaatttaca cgatgagttt ctagaggacg
    25321 ttctacagaa tctgccacac tagctacagc agctaaatga aaaatatagg caaactgata
    25381 ctgtttcatg attctttcca tcaattgagg atctgataca tccccctcaa tgaaagtcac
    25441 ttgtttctcc atatccaagt tttctttttt tcccattgat aaatcgtcga ctactacgat
    25501 tttgttttct tttcccaaat aattcgctag cgttgaaccg ataaaaccag ctccaccagt
    25561 aatcaatata gtatccatca aattcactcc aattatgcat tctcatactt aataaagtta
    25621 gtttaccatt tttatattgt aaaaactata aaatttgttg aagttgggat aactagtttt
    25681 cttttctact ttatatacct aaaaatagtt tcaattagtt actccttctt ctttagatat
    25741 agttttttgg atagaaccca gatatcccca aaaaataaat gaacctatcg tactaaccaa
    25801 aatgagttct gtgataaata atgccgaaac tgctattgta agaatcatta aaatagcaaa
    25861 aaagtgctta tcttttacta gatttggttg tgtgaaacaa taacgcaaag ttttaaagca
    25921 ttggataaat aagaaggtta acaatgtcaa tagacctaat cctcctgtag cagccataac
    25981 taagaaaata aaattatgag cagttaaagt atcttttccc attaatgtat atggtagttt
    26041 atcatgtgcg tattcaatat agtatcctgg agaaccgccg gatatcggct tactcttgaa
    26101 tatttcaatt gcacttttcc aaagtccaaa ccttaaatta gaaacatccc cgccatcagt
    26161 gtcagggcgt tttaaatcaa ctttattgct gctagttgtt cctgatgaat cagatatatc
    26221 tttttttatt tctgtaatat gcggaacatg tgcccatgct tgagcagtta tatctgttac
    26281 ataatacatc gcaattatac ttagcaatcc tactactaat gcagttagcc atctttttaa
    26341 agtattagtt ttaagaaaac gactactata acttgtaaag aaactaaaaa caaaagttgc
    26401 tattaataaa caaactaatg tggtgcgtga gcccgataaa gcaataaacg agaattgaat
    26461 aaaaatatta attgcaaaaa atattttaaa tagaagattt aaatgcttgt aagtgaccaa
    26521 ataatatata gcaataatga tgacaattaa tgatataacc gcaccaaaat ttggatctgt
    26581 atatacacca aataaacggt tgcctaacca ccctaaacga actgggtaat acttatattt
    26641 tattggaatt tttatgccat attgaatgac aaaccagtat aatgatacta aattagctaa
    26701 aaaccatacc aatgaaagta tccaagtaaa tactttgaaa aaaaagttac tactgtagtc
    26761 tttgccaaac tgataaatca taaagaaaaa gattaattgc cacatgatta acttgatatt
    26821 ttcgctatat ccatatttta aattcattaa actgcttact agcatcgcgc caataaaagc
    26881 aattaatagc caatcatatt ttttaagatt tttttctcta aatgcaataa ttgcatctat
    26941 aatgatcaag acaactcccg ctattccagc aggccaaaaa agtaaagtat catatctagt
    27001 aggtaatagt aaatacgggc ctattgctcg taacattaga ataaaaccaa aataaataaa
    27061 ataccaacct ttcacatatt ttaaaaattt ttcattcaac atttttcctc catccaccta
    27121 tttctcataa acatttctta tcctttttag gataaactgg ttctttcaat tctaacaaaa
    27181 aaatgctaaa ctaacaatgt atattaatga atttcgagag gtgaaagatg aaaagagtaa
    27241 ttacttacgg tacctttgat ttgcttcact acgggcatat caatttactg aaacgagcaa
    27301 aacaatatgg agattatctt attgttgccc tttctacaga tgaattcaat tggaatgcga
    27361 aacaaaaaaa atgttatttt agttatgaaa aaagaaaaca gttacttgaa gctattcgct
    27421 acgttgattt agttattcct gaagaaaatt gggaacaaaa agtaaaagat ataaaagaat
    27481 accatgcaga ttatttcgtg atgggcgatg attgggctgg acaatttgat gaacttgaac
    27541 aactaacaga tgcacaagta atttatttac caagaacacc tgagatctct acaactcaaa
    27601 taaagaacga acttaattct ttaaatagtg ataaataata ttttttaaac aatgaaagaa
    27661 caatatatct ggataagacg taaaaaaatg gctctataat ttctaggact gatagaattt
    27721 tctgtcgatc ctagattttc ttgtaaaaaa aacagacata caaaaatttc gataaaatag
    27781 agtcgtctaa aacatcaatc tgaaaggaag attttgtata tccatttctc gttctatcaa
    27841 agagcacttc actattaaag ataaaaatat tgaaattaat tcttttcaaa aaacttttta
    27901 taaggacaag aaagccatcg taatcgaagg tacattagtt cgcactttta gacattgtct
    27961 attttgtaaa gaaactgcga agcagctcgt gaaaaacggc aagaagattt caatgattat
    28021 cttaaatcgc agtgggaata aataaaccta tttgcgtctt agaaaacaac attaccacta
    28081 ccaatcatgc ggaaaatatt ttactgctca tacgtatctt gtcgttcctt catgttttat
    28141 ttcgaaacaa gttcattaca cgatattaga agagttgacc gagcgtcaag cgttaaagac
    28201 acttacgaaa cgttgtgatc tttcagtaac gacatttcaa cggacattat ctgctctatc
    28261 ggcaatgctg cgacagatgc tagactattt acctcattgt ttactattcg acgagtttcg
    28321 ctctctttca atttcccatg gaaagttctc cttttcttgt atgaatagag agacagggaa
    28381 gctctttgac attttgtcca gccggagaaa aaaagatttg attgcttatt tcatgcgttt
    28441 tacaagaaaa gctcatatgg gggttcgata cattgtcaca aatatgaatg ccccttattt
    28501 ttcattagtc aaagagtcct ttccaaacgc ccaaatcatc attgattgct ttcacattgg
    28561 acaacacttg aacaagtttt tcgatcgtgt tcgaaaacgt gtgatgaagc agttgaatca
    28621 aaaagacact tcacaagcta agtactatcg acaattgaaa cctttgtata aattacttct
    28681 aaaatcagat gacgagttgg attacacaac attcaagaaa tggcagaatt ttcaatggcg
    28741 ctatttaaca gaaagtgaag tagttgatcg tctaccctca atctcttatg aacttaaaat
    28801 tgctaagcag tttatcaaaa gctgcttagc aattatcaca agaaacagct agctacttcg
    28861 agaaacgacc aaagaagttc cctcgaaact tcactatctc aaaaagcctt tttcatatct
    28921 gaaaaaggct tttaactagc attttcgaaa aagtattcta ataacaaaat tagagaatct
    28981 tcgcacacat atcaaaacat tgaaacgatt tgcttatggg tttcgcagtt ttaccaatat
    29041 gcgcttgtgg atttttctaa tcaacaggtt aatcagctat gcattaaaag ctagtaactc
    29101 ttactaactt tgttgttcat aaatgacctt atcagcccga tttgacaaag aactactttt
    29161 ttacaacatt attttatttt cgagtaatct ttcttatttc atttcgtata tctggaaaaa
    29221 gaaaataaac aaacggaaga taaataatga aaatcattat tgatttaatt gccatatcta
    29281 aaaatgaaga agagaataat tgtggaagaa agtaataggc ccaagtagtc aaagtaagat
    29341 tgactataaa aaataagata acgttcttcc caaagaaata ggatattttt atttcttttc
    29401 gtacaaaaaa tactctaaag aaaaagacta ataattctac taccacgagt acgatcgtag
    29461 ccgctacagc gccataagta ggcacaaaag ctacatttcc aataatactt acaatcgcac
    29521 caatgatcat tggataagca tactccttat ctttttctat agcaattgca aattggttag
    29581 caaaaactcc accaattgga ctaacaacta taattagaca agaaataagc atgtttattg
    29641 tcatcggttt gaaattctcc ccaaaaaacc atggaacaaa ttcttttgta ttgataaaca
    29701 ccacactaaa aagaattaat gcaatgataa tcgtgtaatc aaaagatttt tttgtgtaaa
    29761 ctaaaatatt ttctgatttc ccctctgcct gtgctttaga tattttaggc aacataacaa
    29821 tagtaattga tgttaagact gccaatataa tacgagcaat tttttgagac tgatcataat
    29881 aagataattc cgttggatcc acaaaatacc caactatcgt tttgtctaaa gttgtgtaca
    29941 cttgcataaa caactgagga gtcaaaataa taaaagctgg tttccacaac tgtttaaaaa
    30001 cattagagtt aaagattggt ttacctactt ttccgtggat acttaaccag aaaactaaac
    30061 ttcctaaaaa gttagtaatt gaagtaatga acacgtaaat ccacaaatca tctttagttg
    30121 aaacaaaaat gattattaac aataccgatg ataatttgat aactgtattt ctaataacca
    30181 ctttcgaaaa ttcttcaatt ccttgaaaaa accaagaaat atctaatgtt agccctacaa
    30241 taaatatcaa ttgaattaag tataaatttt tatactttac at
//
LOCUS       DAZKCN010000024        30204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000024 DAZKCN010000000
VERSION     DAZKCN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.024"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..1039)
                     /locus_tag="ACG8K3_001006"
     CDS             complement(164..1039)
                     /locus_tag="ACG8K3_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9144904.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1148..2917)
                     /gene="aspS"
                     /locus_tag="ACG8K3_001007"
     CDS             complement(1148..2917)
                     /gene="aspS"
                     /locus_tag="ACG8K3_001007"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9144905.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2932..4224)
                     /gene="hisS"
                     /locus_tag="ACG8K3_001008"
     CDS             complement(2932..4224)
                     /gene="hisS"
                     /locus_tag="ACG8K3_001008"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9144906.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4618..5565
                     /locus_tag="ACG8K3_001009"
     CDS             4618..5565
                     /locus_tag="ACG8K3_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9144907.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5654..6913)
                     /locus_tag="ACG8K3_001010"
     CDS             complement(5654..6913)
                     /locus_tag="ACG8K3_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP9144908.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7084..7530)
                     /gene="dtd"
                     /locus_tag="ACG8K3_001011"
     CDS             complement(7084..7530)
                     /gene="dtd"
                     /locus_tag="ACG8K3_001011"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9144909.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7541..9754)
                     /locus_tag="ACG8K3_001012"
     CDS             complement(7541..9754)
                     /locus_tag="ACG8K3_001012"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9144910.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9987..10751)
                     /locus_tag="ACG8K3_001013"
     CDS             complement(9987..10751)
                     /locus_tag="ACG8K3_001013"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9144911.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10752..11699)
                     /gene="prmA"
                     /locus_tag="ACG8K3_001014"
     CDS             complement(10752..11699)
                     /gene="prmA"
                     /locus_tag="ACG8K3_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9144912.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11712..12200)
                     /locus_tag="ACG8K3_001015"
     CDS             complement(11712..12200)
                     /locus_tag="ACG8K3_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP9144913.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12166..12837)
                     /locus_tag="ACG8K3_001016"
     CDS             complement(12166..12837)
                     /locus_tag="ACG8K3_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP9144914.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12975..14261
                     /locus_tag="ACG8K3_001017"
     CDS             12975..14261
                     /locus_tag="ACG8K3_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP9144915.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14333..14527
                     /gene="ssrS"
                     /locus_tag="ACG8K3_001018"
     ncRNA           14333..14527
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8K3_001018"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14605..14889
                     /locus_tag="ACG8K3_001019"
     CDS             14605..14889
                     /locus_tag="ACG8K3_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144916.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14962..15441
                     /locus_tag="ACG8K3_001020"
     CDS             14962..15441
                     /locus_tag="ACG8K3_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9144917.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15664..15807
                     /locus_tag="ACG8K3_001021"
     CDS             15664..15807
                     /locus_tag="ACG8K3_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9144918.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16152..16280
                     /locus_tag="ACG8K3_001022"
     CDS             16152..16280
                     /locus_tag="ACG8K3_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144919.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            16385..17968
                     /gene="abc-f"
                     /locus_tag="ACG8K3_001023"
     CDS             16385..17968
                     /gene="abc-f"
                     /locus_tag="ACG8K3_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP9144920.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18405..19589)
                     /locus_tag="ACG8K3_001024"
     CDS             complement(18405..19589)
                     /locus_tag="ACG8K3_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP9144921.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19614..20621)
                     /locus_tag="ACG8K3_001025"
     CDS             complement(19614..20621)
                     /locus_tag="ACG8K3_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9144922.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20793..21152)
                     /gene="comGG"
                     /locus_tag="ACG8K3_001026"
     CDS             complement(20793..21152)
                     /gene="comGG"
                     /locus_tag="ACG8K3_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP9144923.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21124..21549)
                     /gene="comGF"
                     /locus_tag="ACG8K3_001027"
     CDS             complement(21124..21549)
                     /gene="comGF"
                     /locus_tag="ACG8K3_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP9144924.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21512..21859)
                     /locus_tag="ACG8K3_001028"
     CDS             complement(21512..21859)
                     /locus_tag="ACG8K3_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144925.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(21831..22301)
                     /gene="comGD"
                     /locus_tag="ACG8K3_001029"
     CDS             complement(21831..22301)
                     /gene="comGD"
                     /locus_tag="ACG8K3_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP9144926.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22276..22554)
                     /gene="comGC"
                     /locus_tag="ACG8K3_001030"
     CDS             complement(22276..22554)
                     /gene="comGC"
                     /locus_tag="ACG8K3_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP9144927.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22551..23600)
                     /gene="comGB"
                     /locus_tag="ACG8K3_001031"
     CDS             complement(22551..23600)
                     /gene="comGB"
                     /locus_tag="ACG8K3_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP9144928.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23557..24486)
                     /gene="comGA"
                     /locus_tag="ACG8K3_001032"
     CDS             complement(23557..24486)
                     /gene="comGA"
                     /locus_tag="ACG8K3_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP9144929.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24618..26372
                     /gene="ade"
                     /locus_tag="ACG8K3_001033"
     CDS             24618..26372
                     /gene="ade"
                     /locus_tag="ACG8K3_001033"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9144930.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26470..27132)
                     /gene="trmB"
                     /locus_tag="ACG8K3_001034"
     CDS             complement(26470..27132)
                     /gene="trmB"
                     /locus_tag="ACG8K3_001034"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9144931.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27202..27984)
                     /locus_tag="ACG8K3_001035"
     CDS             complement(27202..27984)
                     /locus_tag="ACG8K3_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP9144932.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28188..29399)
                     /locus_tag="ACG8K3_001036"
     CDS             complement(28188..29399)
                     /locus_tag="ACG8K3_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144933.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29392..30129)
                     /locus_tag="ACG8K3_001037"
     CDS             complement(29392..30129)
                     /locus_tag="ACG8K3_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144934.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
BASE COUNT         8858 a         6424 c         5380 g         9542 t
ORIGIN      
        1 catcccataa atttaaaaaa ataaacatgc tttttttcaa ctttaagaaa atttcaaata
       61 aaaaagcagt ttttctttgc tgattctact atggtagaaa aaaactgcag acaaacgaac
      121 gtctgtctac agcagtattt catgaatttt tgtcaaactc gatctaaatg ccatcctcat
      181 agaaacgacc aagtatctgg acgttttctg caatacttac aaaagcttgt ggatcagaag
      241 ctttcattgc ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc atcaatactg
      301 tctgcttatc atgcttataa gcaccttctg cttcattgat gatcgtgatc cctctacgca
      361 tacagttttg gatttcattt actacttttt ctggatgttt cgttacgatc attacctgca
      421 tcttcttttg tttagtataa acgacatcag ttacttttcc actgacgaaa atagacaacg
      481 tactgtaaaa catatattgc cagccaaata gaattccggc aacaaaaacg atcaatgcat
      541 tgaagtagat cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc gtgatactca
      601 cgatatctag accgcccgat gataggccgt ttctcaacgc tagcccaata cctagtccca
      661 tcacagcccc cccaaacaac gcacagatga ttggatcggt agataggact gtttccggta
      721 aaatatggat acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt gtaaacttct
      781 tcccgatttt taaccacgcc aatacaaaca aaggtatatt cagtaaatac aaagttgttg
      841 ataccggtac ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc tgagctagac
      901 cagtaatacc acttgaataa atatgtcctg gctgataaaa gaaattcaat gcgactgatg
      961 cgatcaacgc ataaacgata gataccgaaa gcttggtcgt gtaatcatga taagcccaat
     1021 tttcgaatgt ttttttcatg gaaaagcccc gctttatatg ttatggcttc attataaagt
     1081 gtttttcaat taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct ttttaccttt
     1141 ttatcggcta ttcttctata ttcgttacat caatattcaa ttcgaacaat tgtaaaggcg
     1201 aaaccatact tggtgcatca ctcatcacat ctgctgcttt tccgttttta ggaaaggcaa
     1261 tgacttcacg aatattatct tctccggcta ataacattgc aaaacggtcc aatcctaatg
     1321 caataccgcc atgtggaggg aatccgtaat ctaaagctgt taacaagaaa ccaaattgtt
     1381 cttccatttg ttcttttgta aagcctagag tttctagtac tttttcttga agctcacgag
     1441 tatgaatacg gatagaacct ccgcctagtt cataaccgtt caagacgata tcataagctt
     1501 ctgcatagac ttttgcaggg tcttcagcca ataacgggat atcttcttct tttggcatcg
     1561 tgaatggatg gtgagcagag acatatcgac cttcttcttc tgaaaattca aattgtggcc
     1621 aatcagttac ccataagaaa ttgaatttcg attcatcaat tagtcctaat tcttttccta
     1681 aacgtgtccg gatagcacct aatgcagcag cgacgatttc acttttatct gcgccaaaca
     1741 tcagcaagtc acctggtttt gcatctgttg ctttaatgat tgcttctgtt gcttctccca
     1801 tgaatttagc aattggacct ttcaatccgt cttcttctac tttcagccat gcaagcccct
     1861 tagctccgaa ttgtcctaca tattgaccta actgatccat gtcttttcgt gagtagcgat
     1921 ctgcagcgcc tttagcattc aatgctttca cgacaccgcc gttttcaaga gccatttgga
     1981 agactttgaa ttctacttct ttgactgtat cgcttagatc gatcaattcc atatcaaaac
     2041 gtgtatccgg tttgtcgctg ccgtagcgag ccattgcttc atcataggtc atacgaggaa
     2101 atggcaatgt gacttcgata ccgcgaactt ctttcattac cttcgcaatc aatccctctg
     2161 tgtaagtttg gatttcttct gctgttaaga acgttgtttc aatatctact tgcgtaaatt
     2221 caggctgacg gtctccacgt aaatcttcat cgcggaaaca acgaacgatt tgataataac
     2281 ggtcgaatcc agcattcatc agcaattgtt taaacaattg aggagattgc ggcaacgcat
     2341 agaaatgtcc agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct tctggtgtag
     2401 atttccctag gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa taatgacgaa
     2461 tcgcttttgt tgtcgcattt cttaatttga tgtttttagt catttttgga cgacgcagat
     2521 ccaaataacg atatttcaaa cgtatttcgt cactgattgc ttgatcatct tcaatttgaa
     2581 atggcggggt ttttgcttga ttcaaaatca cgatttcact tgcaatcact tcgaattcac
     2641 cagtggccat tttaggattt actgcatttt ctccgcgtct tctaacttcc ccagtcactt
     2701 cgatcacgta ttcactgcga catttatccg cgatttccca tgcttctttg ttgatttcag
     2761 gattaaagac gatttgaacg attccttcac gatcacgtag gtcgataaag attactcccc
     2821 caaggtctcg tcttttttgt acccatcctt ttaatgtaac tgtttgttct aattgttgtt
     2881 cggaaactaa tccgcaatag atcgttcttt gtgccattta tttttttcct cctactcgaa
     2941 taaagtcatt tcatcataga cttcatcaaa atgttcatat atctcttcta atggaaaact
     3001 tttttctttt cggcttgcca ttgctttgac attaatcgtt tgactagcaa gctcatcttc
     3061 accgatcacc aatactaatt tggcattcgc tttatctgct gtcttaaatt gcgcttttgc
     3121 tttacggttc atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg cttggaccac
     3181 tttcaatgct tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat ccaactcatt
     3241 aaatgcaggg ataactactt cctctgcctc catagtcaag agtaatcgct cgatacccat
     3301 tgcaaatccg aagccaggtg ttgaaggacc acctagttct tccactagat tgtcataacg
     3361 tccgcctgcg caaatcgttg cttgtgcccc catttttggt gcgtcactca tgatttcaaa
     3421 aatcgtatgt gtgtaataat ctagtccgcg aaccatatta ctgtcgattt cataaggaac
     3481 agccaatgca tccagcatgg aaacaacggt atcaaaatgt ttttgagcag gctcgcttag
     3541 ataatctaag atagatggag cttctgccac aaatttttga tctcgtttgt ctttactgtc
     3601 caatacgcgt aatggatttt catgaaggcg gcgtttagag tcttcactca gctctgattc
     3661 aaaaggtaaa agatagtcga tcaatgcttg acgataggct tgacgcgttt ctttatcacc
     3721 taatgaattg atgactagcc ggatttgcgt gatccccaac tgtttgaaga aatcaagtgc
     3781 catcaccatg ctctccacat cagttgcagg gttttctgat ccaaatgctt ctactccgat
     3841 ttgatggaat tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca ttggtcccat
     3901 ataaaaagtt ttgaacggtt tcgcatactc tgggccgaac aatttatgct caacaaaaga
     3961 acgaacgatt ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc ctttgtcata
     4021 aaaatcatac atctcttttg aaacgatatc cgttgtatcc cctacacttc gggatatcac
     4081 ttcatagtgt tcaaaaatcg gtgtacgcat ttcattatac tgataatctc tgaataataa
     4141 acgtgctgtt tcttcgacaa actgccattt ttctgattcg cctggcaaaa tatcatttgt
     4201 tccttttggt ctttggaaac tcatagtttt ctctcccttt cttagctatg taaataaaaa
     4261 aatcaccctt gttcatcatg aacaagggtg aatgaaattc atacacggta ccacctaatt
     4321 ttgaaagatt tctctttcct cttacgaata acgctcgtac gcgggacggc ttttcactcg
     4381 tccatctcca gagtgtcttt aggctaatga aactttagat cattttcagc ctcgatgatc
     4441 ctctcttgta aagtttcaaa aacttactcg ctctttcatt gacattcgat attacttata
     4501 acctactaaa aaccagtctc aaagtcaaga gatatctgac tatttcccct cacaaaaacg
     4561 ttttagttca gctttttggt attttgtttt agactagaca aaaaggagga atcagctatg
     4621 gcagaaataa gaatcggaca ttcacaagtc tacgcagaac agcttggatt aggagcaaat
     4681 gccgttggag gacataatct ttttgacggt ttggaagatg agacaggaaa acaagtagtt
     4741 cgtacagctt tgaatagcgg gatcaatttg atcgataccg cttatgctta cggaaacggc
     4801 cgttccgaag aactgattgg cgaggtactg aaggaaaaag aatatgatcg ctcacgtgtc
     4861 gtcatcgcta caaaagctgc tcatgtacca aataaagggc gaacatttga taattctcct
     4921 gagtttctca aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga ttacatcgac
     4981 attttttata tccactttcc ggatgaaagc acaccaaaaa atgaatcagt tgctacattg
     5041 cacgaattaa aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt cacgttagaa
     5101 caattgaaag aagcgaatgc agatggatat gtcgatgtag tagaagataa atacagtttg
     5161 atccatcggc aggctgaaaa agaattattc ccttatcttg aaaaaaacaa aatcagtttt
     5221 gttccctatt ttcctcttgc ttccggtttg ctgacaggaa aatatgaact tggtgaggaa
     5281 aaacaatttg gtgaaggaga tcctcgaaaa agaaatccag atttccaagg agaacgtttc
     5341 agagaaattt taaccgctgt cgatgtactt cgccccattg ctaaaagata ccaagccact
     5401 cctgcgcaac ttgttttagc ttggtacatg aagaatccac gagtgtctgt agttatacct
     5461 ggagctaagc gtccagagca agtatctgac aatgtccaag ctttagattt gcatttatcc
     5521 aatgaagatt atcaaaccat tgatgaagca tttcgtggat tttgatcaat tgaccttatc
     5581 aatttatagt aatatgaaaa aaggaagatc agcactgttt gatcttcctt tttttcgata
     5641 agtttatgct aatctaattc ttgtaaagta gcgctcggct attcggataa gtcggaaact
     5701 gattgaatgg aatcgtccaa tcttgttcag gtacagagaa ctcaaactta tttactagct
     5761 gatcggaaaa cacacgtaga ctatgaagag tgatccattc tcctgcacag cgatgcattt
     5821 gtcgaaaatt tcccccgcct tgtgcaatca tctcatattc ttctttatag gaaatatctt
     5881 ttgctttccc tacataccgt ttgatcataa aggaatctgg cgcttcaact gttcgttcat
     5941 catggtctgt tccatataaa tctaaaataa cccaactgcc ttctggtata cgataaccat
     6001 ctacttctac ttctttcagc gaaatcgcag gtagcattgg gaagaaagga taatagcgtc
     6061 tcatttcttg aataaacgga tcttgtaacg tcgaaaagtc attttttaat tgctgataca
     6121 aatctggtct actgaaaagt gcatgtccca tcaatgccgc ccacacagtt agagcaaccg
     6181 ttggacggat gatgttcaat aattcaacag ctgccacttc cagaggtaaa agctgaccat
     6241 ctaaatctgt ggcattggca aaagcatata atgccacatt ttcttttcca gccacaggat
     6301 tttgtcgagc ttcttcaatc aagccttgcg cccatttttc tgattttttg cgattttcga
     6361 caccttttat gtgatcaatc ggagaagtaa acgttccgct gatcatagag atttgatatt
     6421 ctgctaattt ttcgacctca tctttagaaa tcgctgataa attgatacct gcccactcgc
     6481 aaatagatgt aaagaaaacc atcttactta aatcaaatag ttcaaattga ccatgctgtg
     6541 cttcaagtgc ttgtgtcaga ttcttatcta agatacgatg ataatcttcc attctttccg
     6601 gtgtcattag atccataaaa atatttttcc gatggtgatg agcagctcca tccaaagtct
     6661 gtactccacc ttgaccaaaa agtgtcttca atactaattt tggcatagca ccttttcgtt
     6721 tgaaattcct tggatcataa aatacttttg ctgctgatga tccgtagatc gtgatagctt
     6781 ctttattgaa aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt aactcctcca
     6841 acatgttgta tcctttttga tacaattttt ttaaatctgt gattttgata tctactactg
     6901 gtacttcttt catcttcagc aacctcctat aaaaaataac ttgtataatt taagcttaac
     6961 tttttttccg actgctgaca aaagaagcgc tttttgcaga caaaaaagcc acttaagtta
     7021 gagtttatca gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt
     7081 actttatttg tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac
     7141 cgacatatct gctccgaatt gtccggtagc gactgtgatg ccgcgacttc tgattccttc
     7201 attgaatgat tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg
     7261 tcgatttcct tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc
     7321 tcccacatct tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg
     7381 tgcaattttt tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa
     7441 taaaacaagt aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc
     7501 tttacttacc cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta
     7561 cactgtagac atcaggtact gctttgattt tatccacgat actctttagg tgggataaat
     7621 tctggatacc aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa
     7681 cgctgttgag atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg
     7741 aacgattata gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat
     7801 cttcccattc gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg
     7861 gacaatctcg acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat
     7921 caccaggaat cggattacag cagcgactga tacgaattag taagttttct accccttgaa
     7981 tgacaacgcc accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca
     8041 tgatttcttc tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag
     8101 tcagacgatt ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat
     8161 catcttctgt ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa
     8221 tatctttagg cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga
     8281 tcacattttc ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc
     8341 tagtagcaac taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga
     8401 tctcaatgat atctccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt
     8461 ttgcaccagt ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc
     8521 cggatccttt tggtaactca gtaacgtctc cttttggtgt gaagacgtag actttatcac
     8581 taaagatatc tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa
     8641 gttcgagtat ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt
     8701 cattttttcc ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca
     8761 tttcctgtgt tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat
     8821 gcaaagattg gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca
     8881 ttggtttcca ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta
     8941 cgatcactcg aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca
     9001 ttttacgata gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca
     9061 gctcttctgt cgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt
     9121 ctctcttcgt ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa
     9181 gtgctgtatc ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg
     9241 catagatttc taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca
     9301 acgtacgcat gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag
     9361 ccattgctag cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt
     9421 ttattttccc taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt
     9481 ctctcagatc ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag
     9541 ccactgtatg gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa
     9601 taatgtaggg ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc
     9661 atgctttctc aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga
     9721 caccgggtcc cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc
     9781 ggtaaaacgg gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta
     9841 tacgtttttt tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc
     9901 attgtataaa taattgtttc tattatacaa attgttgact attttttcaa ttctttctgt
     9961 cgatactctt ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga
    10021 tagctagcag cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct
    10081 aatccacaaa gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca
    10141 ccttctggtc caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg
    10201 gctgcatttg atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca
    10261 taatcatcta gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg
    10321 atctgccgat gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt
    10381 ttcatacgtt tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa
    10441 gcttgcgctc ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga
    10501 tagccactcg ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt
    10561 tctttcagat aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga
    10621 tcattgaatg ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg
    10681 tgatagtttt ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa
    10741 taccgctgca tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc
    10801 tggaaaattt gatccacgat aaacccttgt tctaacatgg catccagaac catttgcttc
    10861 ttctcttcaa tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca
    10921 tctgggatca tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca
    10981 atctcgattc ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc
    11041 atatccatat tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca
    11101 tcttttgcac caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct
    11161 agcaatgttt caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg
    11221 ggatgagtgc ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga
    11281 tcttctgtct ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc
    11341 ggatgatagt atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt
    11401 actttatttt ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata
    11461 aaaggcaaga tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct
    11521 tctttcagcg atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa
    11581 tgttcaacat ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata
    11641 ttagcaactg cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata
    11701 tctgtcactc cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt
    11761 acttctacta attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca
    11821 tcgtcttcat ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga
    11881 tattccacta aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt
    11941 actgctagat aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg
    12001 tcttcaaatt cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat
    12061 tgtgctttat tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc
    12121 caatctaaag ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt
    12181 aatagatcct tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt
    12241 tctttgtaaa gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca
    12301 tacacctttg tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt
    12361 tttctcaaaa actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg
    12421 aattgctaat gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc
    12481 gccacgattt ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac
    12541 tccttgagga atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt
    12601 gtaaaggtag atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa
    12661 accatagcta tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata
    12721 tccagctaat cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc
    12781 cacttctggc gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt
    12841 tctcacctac tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga
    12901 aggctgttct ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga
    12961 aggaggaaaa tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg
    13021 aagtcgttgg tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg
    13081 ctcgcatgct ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg
    13141 caagtgcgat cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt
    13201 caaaaaagga tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct
    13261 tattggatga agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag
    13321 aaagtggccg aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc
    13381 cagcgatccg cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc
    13441 agttggcagt cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc
    13501 agttagacga agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg
    13561 cattgaatgg gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc
    13621 atcttacact gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa
    13681 atggagatgc tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg
    13741 tggatgctgc acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt
    13801 gccggcgtct gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg
    13861 ctcgtacagt gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc
    13921 cactagcaga cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg
    13981 cattagatgc agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt
    14041 tgagagatag ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc
    14101 ctcatcattt tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg
    14161 ctcagtatta tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt
    14221 accgtataaa agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt
    14281 tctgcgagct ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct
    14341 gtgatgttcg aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac
    14401 ccgtcttgtg tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat
    14461 gggacccacc tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg
    14521 agattaccat cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg
    14581 ttgcttattt tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta
    14641 ttattattcg ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac
    14701 aaaaatgata aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc
    14761 gtaatgggta ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg
    14821 atcgtaatta ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca
    14881 aagcagtaaa taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat
    14941 tgacttgagg agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat
    15001 ggttctgatg aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac
    15061 ggagaacttc tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt
    15121 gatggaatgc ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa
    15181 agcaatgcga agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca
    15241 aaacaaatta ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga
    15301 gccacaggat tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt
    15361 agaaacgcag cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct
    15421 aaagaccaca ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc
    15481 acaaaaaacc gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt
    15541 aaaataaagg gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc
    15601 gattagtcgc cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc
    15661 aatgtggaag tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc
    15721 gattttatcg ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct
    15781 acattcggta aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac
    15841 gatactcctg aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg
    15901 tattaagcaa atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga
    15961 tttcgcctga acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct
    16021 cagtcagata aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc
    16081 agcgctagtt gaactttctg acactttcag gtacactgga aaagacaact acacgaagga
    16141 gggaaaatac tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc
    16201 atatcgcatt tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca
    16261 aaacagttga attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct
    16321 gagtttaaat aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga
    16381 gatcatgatc atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga
    16441 ggatttatcc tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac
    16501 tggcaaatca acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat
    16561 cagtcgcaaa aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc
    16621 gaccgtcaac cactacttgc tccattctgc cgctgagata caggacctca aacagcaaat
    16681 gagtcgctac gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg
    16741 ttacggtgag ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat
    16801 tgcagcgatc ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact
    16861 tagcggaggc gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt
    16921 acttttactc gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa
    16981 ctatttacag aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga
    17041 tcaagtcacc acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa
    17101 ctacagccgt tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga
    17161 actgcagcaa aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc
    17221 acatgaagga gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc
    17281 gaaactgaca ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa
    17341 tggtggaaaa tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga
    17401 tcaagtacta ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat
    17461 cggcgacaat ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc
    17521 ttacggtggc accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct
    17581 gcagtttgcc gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga
    17641 agaagtcgct cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg
    17701 gatcaaagat ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga
    17761 aaaaatcaac gtcctgattt tggatgaacc aaccaatcac ctcgacagct atgctcgaga
    17821 agaaattgaa gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg
    17881 ctactttttg cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg
    17941 ccagccgctc ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc
    18001 atacctgtgc tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg
    18061 atcaatgaat gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca
    18121 gcccacgagg ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag
    18181 atgaatgaaa ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt
    18241 tcccttttga ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga
    18301 aattgaagtt aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc
    18361 cctcttgcat gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc
    18421 tccacatcac gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt
    18481 gattcttttg tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt
    18541 tcacaaccaa accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg
    18601 ccgataccag cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg
    18661 atgtattttc ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca
    18721 tagttctttt caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat
    18781 ttcttgttca aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat
    18841 gggagaacag ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt
    18901 gtgaatccca tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca
    18961 tttcctaagt ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc
    19021 gcacgttccg acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa
    19081 tcttcataat aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg
    19141 aaagatgtat cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg
    19201 atccccatta agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg
    19261 tccattactt tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca
    19321 cggtgcccta ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga
    19381 tctaacagca tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt
    19441 tgattatcgc cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc
    19501 cctttcgcaa ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc
    19561 ccagcattga ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga
    19621 gcagatgatt tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt
    19681 aatgaagcaa gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc
    19741 tgtaagatca aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga
    19801 atcattcctt gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc
    19861 gtttctaaaa atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc
    19921 attgattgct ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa
    19981 aactcttttg ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa
    20041 tcgacaggat ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct
    20101 tgtgtcaaat cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca
    20161 attccttctg tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg
    20221 cccattcctg ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt
    20281 tcaataagaa aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc
    20341 aaatgttctg tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct
    20401 ggctcgaaag aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct
    20461 tttgtcggca ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt
    20521 tcgacatagg cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc
    20581 aggttttgat taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga
    20641 tatattataa caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc
    20701 gttttcactg ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata
    20761 acagaaaaga gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt
    20821 tttacttctt ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac
    20881 tgttgtaccc actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt
    20941 tcggcaagcg tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa
    21001 tttttgattc gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat
    21061 aaaaacgtac ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat
    21121 tttctactga tcgttttttg tccatatggc atatccatct tctccatttt caaagtgtac
    21181 ataaaaagaa attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat
    21241 aggttgatgt ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat
    21301 atactgttta tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact
    21361 gctcttgagt tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg
    21421 ctgcaaaaat tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag
    21481 actgctcaat atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg
    21541 ttttttcaat actgatcgct tctttctcca tattgacaaa cactttttcg attactccat
    21601 tattatcgac aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt
    21661 gatacatccg ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc
    21721 cacttttctt ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga
    21781 cagaagcgat cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt
    21841 ctttttgtat agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct
    21901 tcccaataaa attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta
    21961 atagaagcat ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt
    22021 ttatctggat tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct
    22081 gtttggatct gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg
    22141 ccgaaaaatt gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa
    22201 gaaaggatcg ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat
    22261 gctaaaacct taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc
    22321 ttcattcaac aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc
    22381 tttttgtgtg tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct
    22441 ataacgcgac aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat
    22501 tagcatttcg atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa
    22561 acctcctcta tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa
    22621 gccgctacgc ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg
    22681 aaaagctgtt ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt
    22741 gcctctccct gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac
    22801 tgctcagcca atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga
    22861 atcagacttt cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct
    22921 tcatgaaaaa gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc
    22981 ggtccaatca ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc
    23041 agacgatacc ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac
    23101 caatgaaaga cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca
    23161 ataagctgag gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc
    23221 aatagcatcg gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga
    23281 aactgtttgg caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg
    23341 atctgtacca gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc
    23401 ccttccttta gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg
    23461 agtaataaat aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt
    23521 tgtgcaaact ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat
    23581 attttcctga gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat
    23641 aaattgataa ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata
    23701 aatgacacca gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct
    23761 gaccaatgta ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag
    23821 tgctgctctt accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa
    23881 gtctggacga tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg ctattttcgg
    23941 attgatctgt agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat
    24001 cacttgcaat ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac
    24061 tggtccgctg aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc
    24121 tcgagaacta aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa
    24181 actctctcgt tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc
    24241 taaagaataa gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc
    24301 caaatactta aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa
    24361 tgttcgtttt tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata
    24421 tacgtcttgg gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat
    24481 ttccattatt ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa
    24541 aatgaattta caaaaaaata ttggcaaaaa ccgaatttat gttatactta taaaaattta
    24601 tcttgggagg cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa
    24661 aaaagcctgc tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa
    24721 tcatggaaaa agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag
    24781 gaaaaagcgt acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg
    24841 tgcatatcga aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg
    24901 gagttactac tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc
    24961 ttgagtttat gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt
    25021 cttgtgtacc tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag
    25081 cccctttttt acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag
    25141 tagcaaataa tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa
    25201 aaattgacgg gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag
    25261 gtattcggac ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg
    25321 gtatgtattt aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg
    25381 cgatcacacc tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg
    25441 atttagtaaa agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg
    25501 atccattaca agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa
    25561 cactaggtgc gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca
    25621 agctgccaat tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc
    25681 aagaccattt atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg
    25741 ctcaaccatt aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga
    25801 tcgaaattat cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag
    25861 gcgaacgatt cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc
    25921 accatcaaac gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag
    25981 cgatagcgac aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg
    26041 aggatatgct gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca
    26101 acaaacagca ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac
    26161 ctttttcgat cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag
    26221 cagctaaaac ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg
    26281 aattgaagat caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt
    26341 gtggaaaaac cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac
    26401 atctgtaatg ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga
    26461 aagttagttt cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata
    26521 aatacgttga ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa
    26581 ttgactttga tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact
    26641 tgccagagaa tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg
    26701 acgaagaatc tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg
    26761 acgttttttt ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc
    26821 gaaatactct gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg
    26881 ttcgatcaac ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata
    26941 attgatttca ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat
    27001 ctcaatgtgg attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt
    27061 tggatcacta attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt
    27121 tcggacacgc attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaatct
    27181 ccgcagagtt tgggccaaac ctcagccact gaaaattcgt tttaattgta aaatttcttc
    27241 gttcatccgt cgatgatctt cacgtttgca atatctggtg atttcttgga gcaaattcat
    27301 ggccgcatac caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc cataactgat
    27361 cagccactga ttccagctag acagtggcac ataatttcct aaaatcgttg caatatccac
    27421 cgcagggtct gcaaacatga ttgaatccca atcgactaga tataaataat ttttacatac
    27481 taaccaatta cgatggttta catctccgtg tactactgat aagttttcct tatctattga
    27541 cgggatctgc tcacgaagaa aacgatggac aatttgcaga aattgatttt tctggagttc
    27601 ttccggtaat tctttttcat atttttgcag catcttctcc ggcgtcatga cacgaccacc
    27661 gatacgggtc agcatggttt taagagaatt tgaatgatgt aaatggtaca aaacatcaat
    27721 cacgtcgttt ctttgaccaa tctcattagg agtcagcaag cgaccttcca gccactcttg
    27781 tgctgtcaaa gtatcgccat tccccgttcg ttttgtccaa acaagttttg gtgtaattcc
    27841 ttcccttgaa agagcagcca acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc
    27901 atcacgcatg cctatgtatg tttttcctgt gtcgcctttg atgggacgta accgccactc
    27961 ttggtctaac tgaaactcca tcatgcattc tcccgtcctc ttactctttg ttttgcagta
    28021 cttattattc tagtcgcctg aacactacac gtcaagcaca tatacgttct ttttcattta
    28081 attgccattc aaaaaaccag cttttctcaa aaaaaataga acgaacagca ttcttactgt
    28141 caacgctcta tttttcattc tcatttttac cctacacagc tttgttttca gtctgacatc
    28201 ttcttcaaac gagttggtac atatagttta acaaagagca tagtaacaat caaataagcc
    28261 gctagcggaa cccatcgctg ctgcccatct aatacgactg cagcaatcaa accaaaaata
    28321 accgacgcga taattaatac ccatgtgagt agatactgaa tcgcctgctt cttctgattc
    28381 ttatggattg gataaagctg aacgagaacc atatagcgga actggttcgc taaaggtaac
    28441 agttggaaac cgatcaaata taaaaataag aaaccgataa tcagactaaa ataaaagtcg
    28501 actactccgg caatcaataa cccacctata attactaatc ggaaataaag accactaaaa
    28561 tcagaacctc ttgccaatct tcttgcaaac aagtaaagat aagtgttttt tgattctctt
    28621 ttaatatgag tcaatatagg atccaaatag cgtcttcttt tgatctttgc cgtaatttca
    28681 ggaacatctg tgaataaatt gataaattga tagatctgat gcaacctatg ttcttctaca
    28741 tgcaccattt tctcccaatc caatcctttt tcttgttgct tccacaaaaa ccagtaatat
    28801 agacttgcct gtactaatgc acctgctatt cctgcccata ttagccaata aaatgataga
    28861 aacaaaaaga tcagagaaga aaaaaaccag cctaatttcc aataacgaat aacttgttga
    28921 tctagtcgaa acaattctgc tctcttgatt tgcagatgac tgaatttcaa actccataat
    28981 atgatcagat agacgaaaaa catcgaaaag gattgctccg tactaaccac cactaatggc
    29041 atggccaatc cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa
    29101 gctcgttcca aataggtttg catttctttt tctttaggaa gtaaaaaaac aatatctgct
    29161 gcctctgcaa aagtagccat cttacccacg aataaacaga gcatccaaaa tacagagatt
    29221 atgagccctc caattgcaaa cggtggctgc agggttttca accaattaga ataatataat
    29281 cccacaccgc caatcaagaa aagacaaact aaaacaaaat gatcgttcaa tacataacgc
    29341 atatatttca tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg
    29401 tgcctgctcc tttgttaaag caatatagat ttcgtccaaa gaagaatccg gcagctgaaa
    29461 ttcttttctc aacatctcaa gagaaccgtc tgctcgtatc tctccgttat gtaagaccac
    29521 aaaacgatca caatattttt ctgcagtagc taaaatatgc gtagacatca aaataccggc
    29581 tccttctttt ttcatttcta ccatcaattc aagcaatgca ttgatagcta gtggatccaa
    29641 gcctaaaaat ggttcgtcga tgatatataa agaaggctcg atcaaaaagg cgcataagat
    29701 catcactttc tgtttcattc cctttgaaaa attagctggg aaccagtcaa gacgattaga
    29761 taaccgaaaa gtctccaaca acttttctgc tcttttcatt gctatatcca taggtatatc
    29821 gtaggccatc gcagtgactt cgatatgttc tctcaatgtc aattcttcat ataaagaagg
    29881 agtctctgga atatacccaa tttgtttgcg atattcttcc gtatttttct ttaaggatag
    29941 atgattgatt tctatcgttc cttttgaagc ttccagcaga ccgataatgt gcttgatggt
    30001 cgtacttttc cctgctccgt tcagtccaat caaccctact atctctccag gctgtacatc
    30061 gaaactgata tcttttaaca cagggagatg tccgtacccc cctgtgacat gatcgataat
    30121 taaactcatt ctttttcctc cgccatttat ttttatttat tataccacga agaaaatgac
    30181 ggtattttgt ttttttcctt acaa
//
LOCUS       DAZKCN010000025        29109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000025 DAZKCN010000000
VERSION     DAZKCN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.025"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1030)
                     /locus_tag="ACG8K3_001038"
     CDS             complement(<1..1030)
                     /locus_tag="ACG8K3_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9144935.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ACG8K3_001039"
     CDS             complement(1112..1801)
                     /locus_tag="ACG8K3_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9144936.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG8K3_001040"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG8K3_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9144937.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG8K3_001041"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG8K3_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9144938.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG8K3_001042"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG8K3_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9144939.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG8K3_001043"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG8K3_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9144940.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG8K3_001044"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG8K3_001044"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP9144941.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG8K3_001045"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG8K3_001045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP9144942.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="ACG8K3_001046"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="ACG8K3_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="ACG8K3_001047"
     CDS             complement(7635..8522)
                     /locus_tag="ACG8K3_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9144943.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG8K3_001048"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG8K3_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9144944.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG8K3_001049"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG8K3_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9144945.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG8K3_001050"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG8K3_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9144946.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG8K3_001051"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG8K3_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP9144947.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ACG8K3_001052"
     CDS             complement(10453..10956)
                     /locus_tag="ACG8K3_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9144948.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ACG8K3_001053"
     CDS             11553..12518
                     /locus_tag="ACG8K3_001053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9144949.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ACG8K3_001054"
     CDS             complement(12617..13789)
                     /locus_tag="ACG8K3_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144950.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ACG8K3_001055"
     CDS             complement(13776..14921)
                     /locus_tag="ACG8K3_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9144951.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ACG8K3_001056"
     CDS             complement(14935..15867)
                     /locus_tag="ACG8K3_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9144952.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ACG8K3_001057"
     CDS             15984..17063
                     /locus_tag="ACG8K3_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9144953.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ACG8K3_001058"
     CDS             17060..17689
                     /locus_tag="ACG8K3_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9144954.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ACG8K3_001059"
     CDS             complement(17799..18026)
                     /locus_tag="ACG8K3_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9144955.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ACG8K3_001060"
     CDS             complement(18129..20717)
                     /locus_tag="ACG8K3_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP9144956.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ACG8K3_001061"
     CDS             complement(20737..21396)
                     /locus_tag="ACG8K3_001061"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9144957.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG8K3_001062"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG8K3_001062"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9144958.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ACG8K3_001063"
     CDS             complement(22169..23035)
                     /locus_tag="ACG8K3_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP9144959.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ACG8K3_001064"
     CDS             23144..23785
                     /locus_tag="ACG8K3_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9144960.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG8K3_001065"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG8K3_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP9144961.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ACG8K3_001066"
     CDS             complement(25226..26125)
                     /locus_tag="ACG8K3_001066"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9144962.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ACG8K3_001067"
     CDS             complement(26127..26924)
                     /locus_tag="ACG8K3_001067"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP9144963.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ACG8K3_001068"
     CDS             complement(26902..27606)
                     /locus_tag="ACG8K3_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP9144964.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ACG8K3_001069"
     CDS             27737..28312
                     /locus_tag="ACG8K3_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP9144965.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="ACG8K3_001070"
     CDS             28430..29107
                     /locus_tag="ACG8K3_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP9144966.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DAZKCN010000026        28043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000026 DAZKCN010000000
VERSION     DAZKCN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.026"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..694
                     /locus_tag="ACG8K3_001071"
     CDS             23..694
                     /locus_tag="ACG8K3_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9144967.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="ACG8K3_001072"
     CDS             753..1193
                     /locus_tag="ACG8K3_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144968.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="ACG8K3_001073"
     CDS             1205..1873
                     /locus_tag="ACG8K3_001073"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9144969.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="ACG8K3_001074"
     CDS             1937..2560
                     /locus_tag="ACG8K3_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP9144970.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="ACG8K3_001075"
     CDS             2616..3002
                     /locus_tag="ACG8K3_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP9144971.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="ACG8K3_001076"
     CDS             3215..4606
                     /locus_tag="ACG8K3_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP9144972.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5217..5585
                     /locus_tag="ACG8K3_001077"
     CDS             5217..5585
                     /locus_tag="ACG8K3_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9144973.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5765..6706
                     /locus_tag="ACG8K3_001078"
     CDS             5765..6706
                     /locus_tag="ACG8K3_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9144974.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6775..7506
                     /locus_tag="ACG8K3_001079"
     CDS             6775..7506
                     /locus_tag="ACG8K3_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9144975.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7650..9830
                     /locus_tag="ACG8K3_001080"
     CDS             7650..9830
                     /locus_tag="ACG8K3_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9144976.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9850..10332
                     /locus_tag="ACG8K3_001081"
     CDS             9850..10332
                     /locus_tag="ACG8K3_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP9144977.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10348..11109
                     /locus_tag="ACG8K3_001082"
     CDS             10348..11109
                     /locus_tag="ACG8K3_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9144978.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11240..12574
                     /gene="murC"
                     /locus_tag="ACG8K3_001083"
     CDS             11240..12574
                     /gene="murC"
                     /locus_tag="ACG8K3_001083"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9144979.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12881..13366
                     /locus_tag="ACG8K3_001084"
     CDS             12881..13366
                     /locus_tag="ACG8K3_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP9144980.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13359..14042
                     /locus_tag="ACG8K3_001085"
     CDS             13359..14042
                     /locus_tag="ACG8K3_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9144981.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14168..16813
                     /gene="polA"
                     /locus_tag="ACG8K3_001086"
     CDS             14168..16813
                     /gene="polA"
                     /locus_tag="ACG8K3_001086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9144982.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16858..17694
                     /gene="mutM"
                     /locus_tag="ACG8K3_001087"
     CDS             16858..17694
                     /gene="mutM"
                     /locus_tag="ACG8K3_001087"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP9144983.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17658..18287
                     /gene="coaE"
                     /locus_tag="ACG8K3_001088"
     CDS             17658..18287
                     /gene="coaE"
                     /locus_tag="ACG8K3_001088"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9144984.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18339..18671
                     /locus_tag="ACG8K3_001089"
     CDS             18339..18671
                     /locus_tag="ACG8K3_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9144985.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18983..19474
                     /gene="nrdR"
                     /locus_tag="ACG8K3_001090"
     CDS             18983..19474
                     /gene="nrdR"
                     /locus_tag="ACG8K3_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9144986.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19497..20870
                     /locus_tag="ACG8K3_001091"
     CDS             19497..20870
                     /locus_tag="ACG8K3_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9144987.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20870..21796
                     /gene="dnaI"
                     /locus_tag="ACG8K3_001092"
     CDS             20870..21796
                     /gene="dnaI"
                     /locus_tag="ACG8K3_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP9144988.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21925..22365
                     /locus_tag="ACG8K3_001093"
     CDS             21925..22365
                     /locus_tag="ACG8K3_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9144989.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22509..23210
                     /locus_tag="ACG8K3_001094"
     CDS             22509..23210
                     /locus_tag="ACG8K3_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP9144990.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23207..24328
                     /locus_tag="ACG8K3_001095"
     CDS             23207..24328
                     /locus_tag="ACG8K3_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9144991.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24343..25575
                     /gene="pepT"
                     /locus_tag="ACG8K3_001096"
     CDS             24343..25575
                     /gene="pepT"
                     /locus_tag="ACG8K3_001096"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9144992.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25707..26615
                     /locus_tag="ACG8K3_001097"
     CDS             25707..26615
                     /locus_tag="ACG8K3_001097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9144993.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26650..26922
                     /locus_tag="ACG8K3_001098"
     CDS             26650..26922
                     /locus_tag="ACG8K3_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP9144994.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26933..27979
                     /locus_tag="ACG8K3_001099"
     CDS             26933..27979
                     /locus_tag="ACG8K3_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP9144995.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9431 a         4843 c         6111 g         7658 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttaggagg aaacagatgt
     5221 ttacgaatga aataaaaatc atgctttatg tggacgatgt agaaaaaaat gcaacgtttt
     5281 ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat ggatgggacg cttataattg
     5341 aagtaggagt tgaggacagt caaacctcat ttctgttata tgatcgagca tttgttgagt
     5401 cccattcacc tggcacagct actagtgccc catctttgat gttttacagt aaagatatct
     5461 ttggactata taaaaaaatg cagacgcaat cagtcacatt aggtgatatg gtacagcttg
     5521 gagaagaata tgtctttaac tttgctgatc ccgatggcaa ctactttgcg gttacaggta
     5581 aataatagaa ataaagactt tgtttttctg tgcgaaacag gaaagcgaag tctttttaat
     5641 tcaaataata atattatgta aacaaaaaac tttccatgcg taattataca caagcaaata
     5701 aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa
     5761 atacatggat tatcaagtac tgctttatta caattataca aaaatcgaag aaccagagga
     5821 atttgccaaa aatcatttag ccttttgcaa atctttggat cttaaaggtc gtattctagt
     5881 agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat
     5941 gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg ttcaagatcg atccaagcga
     6001 aggacatgcc tttaagaaga tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct
     6061 agaagaggac attgatccaa accaagtgac aggtgcctat ctttcaccgc aagaatttaa
     6121 agaagctatt ttagatgaac agacagtggt gattgatgca cggaatgatt atgaatatga
     6181 cttgggacat tttcgagggg ctgtacgccc agatatacgc agttttcgag agcttccaga
     6241 ctggatccgc gagcacaaag aagaatttat ggaaaaacgg gtagtgacct actgtactgg
     6301 tggcattcgt tgcgagaagt tttctggttg gcttgtccgt gaaggattca aagatgtcgg
     6361 acagctgcat ggcggtattg ctacttatgg taaggatcct gaagtccaag gagaattatg
     6421 ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc gatatcaacc aagtagataa
     6481 acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt gaacgatata tcaattgtag
     6541 caatcctgag tgcaatcgtc agattcttgc ttccattgaa aatgaagaaa aacatttagg
     6601 agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc tatgttcaag accatggaat
     6661 ctctccagaa gaagcagaac tgcggtttac agctttaatt aaatagaaaa caatagtttt
     6721 gattgtcgac catttccaat atgcgctata ctagcgttga ggatggtgat ccacatgaaa
     6781 tcattgattt atgctattgg ggatgtccat ggcaaacatg agctttttga aaaacttatt
     6841 cactattatg atccaaagat ccatcagctt gttttattag gggatctcaa tgatcgaggt
     6901 cctcgtacaa aagaatgttt tttattagga aagcaattag tcgaagaaac aggagccatt
     6961 tacctccggg gaaaccatga agaatatttt ttgcaatttc tgcaatcacc ggaggactgg
     7021 tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg
     7081 gcatcggagg aatactcccc aactgaaatc tcgatgatga tccaatcgag atattcagaa
     7141 ctgattaaat ttttagtcca gcgccccttg tattatgaat gggaaaatta tctttttgtc
     7201 catgccggtg tggatttgac aaagaaagac tggcgtaaga caagtctaag agactttatt
     7261 tggataaggg aaccatttca tgaagggaaa aacaatacag gtaagacaat tgtatttgga
     7321 catacgatta caccaatgct ccatggagat atgcagacaa cagacctctg gattcaggat
     7381 cataaaatcg gaatggacgg tggggctgta ttcggagggt cattgcatgg ggttgttttt
     7441 gacaaggaag gtattcttca agatattgaa tatcagaaca acgaaggacc ttggcatgga
     7501 atctgactaa tggaaagaat gaaggagctg tgactttgtt gcggctcttt ttatttttat
     7561 agagaatatt gtttctttca gtagtttaac ccaggataaa aagtatgata gaataaagaa
     7621 gcaattttta gcagaaaaga ggcattttaa tgacggaaaa tttgaaccag acgatcattc
     7681 agcttgtaca aaaagaactt tcagactatc gtcctaagca gttgactact gtacttaatt
     7741 tgttaaatga aggaaacact gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa
     7801 gtcttgatga agtccagatt cgtgaaatcg aagagcggta tgcctatctt gaaaacttag
     7861 aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca agggaaactg actcccgagt
     7921 tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat
     7981 acaaacaaaa acgacggaca aaagcaacga ttgctaaaga aaaaggactt gaaccactcg
     8041 cgaagtggct gatgcagctt acagatggag aggtgcaatc tgaagcagaa aaatatattg
     8101 acaaagaaaa agaagtctct tctgccgaag aagcgttgca cggtgctcat gagattattg
     8161 ctgaacaagt cagcgataat gcaaaatttc ggacttggat tcggtcttat acgtataaca
     8221 aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa
     8281 tgtattatga ctttgcagaa cccgtccata aaatggtttc tcatcgaata ttagctacta
     8341 atcgtggaga aaaagaagat attctgaaag tattctttca agtagatgaa gcagcaattt
     8401 tagcttactt ggatcgccaa atagtcaaaa atcctgcctc tccttcttct tcattcgtca
     8461 gagaagccta tcaggatagt tataaacgct ttattcagcc agcaatcgag cgagaactgc
     8521 gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc tattttcggc gaaaacctcc
     8581 gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt cctgggattt gatccggctt
     8641 atcgtactgg atgtaaacta gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag
     8701 ttatttatcc tcataaacct gcagcgcaag caaaaagaga agctgctgga ccagctttta
     8761 tccaattaat taataaatat caagtagata tggtagctat cggaaatggt acagccagtc
     8821 gggaatctga actttttgtg gctgagcagt tgaaatccgc cgaccataaa acctattacg
     8881 ctatcgtcaa cgaagcaggc gcttctgttt actccgctag cgaaattgca cgaaaagaat
     8941 ttccgcatct acaagttgaa gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc
     9001 ctttagctga attagtcaaa atcgatccta aagctgtcgg agtaggccag taccaacatg
     9061 acgtttctca aaaacgtttg gctgaacaat tggattttgt tgtggaaaca gcggttaacc
     9121 aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt gcagcatata tcaggattga
     9181 ataaaacaac tgcccaaaac attgtcattt atcgtgaaga aaacggagaa ttcaccgcac
     9241 gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc atatgaacaa gcaatcggat
     9301 tcttgcgagt ccctggtggg aaaaacattt tggacaacac aggtatccat cccgaaagtt
     9361 attctattgc aaaagaaata ttaacttcag tccaactttc agaaaaagag cttggtacag
     9421 aagaagcagt agaaaaattg acacgattat ccgtagagaa actggctgaa agcctatcag
     9481 tcggagaaga aacgttagcc gatattttag ctgggctgac acaacctgga cgagatatgc
     9541 gtgatgagat gccagcgccc ttattacgta cagatgtatt gagtatggaa gacttgaaac
     9601 cagggatgga attgactgga acggtccgta atgtcattga cttcggcgca tttgttgata
     9661 tcggtgtgaa gcaagacgga cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc
     9721 atcctacaga tgtcgtgtca gtaggagata tcgtaacagt ttggatagaa caagtcgata
     9781 cgaaaaaagg tagaatcagt ttgacgatgc tttcccctta tgaagagtaa caatgaggag
     9841 taacaaaaat tgacacaaga agaactgcag cagttagtag aagagatatc attaaaaagc
     9901 ttccagcttc ctttcaagca ccaagcaatg ttcaatccac gcttacgtac aactggcggg
     9961 cgctatcatt taaacgatca tcatttagat tttaatgcga agctatttga agagtcagac
    10021 gaagagacga ttgcaggaat catcaaacat gaactttgcc attatcatct tcatctagct
    10081 ggaagagggt atcagcataa agatatggat ttcaagtatc ttttgaaaaa aacaggaggg
    10141 ttgcgctatg caccagcctt gacgaagaaa cagataaaga tggagtatta tcgatgccaa
    10201 gattgttctg ccacgatctg ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga
    10261 agatgtcatg gagaaatcaa atggattgag acaaaaagag aagactttaa tgaagcacaa
    10321 aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac
    10381 tggaatcagc tattaaattt tttagccgca cctgaagagg gaaattttga ggaactgacg
    10441 gcacctacac ttattttagc tggaaactgt cttcctatct tagctgataa agcagcacaa
    10501 atgtatttaa atggccaagt agatcaattg tttttggtcg gaggggtggg gcatgcgact
    10561 agaatccttt atgagaattt tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa
    10621 agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt tgcccgataa ggcttttctg
    10681 atagaatcga agtctacaaa ttctggagag aacgccactt tttcactgga gattcttcat
    10741 tccttaggag ctgtaccaga aaaaatctta ttgatgaatg atccgacact gcaacgcagg
    10801 actcgagcaa cttttgagaa agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg
    10861 ccatttgtgc cggaaatcct tcacttttct gaagagatca tctttacagc aaaagaactg
    10921 aatcatcaat ggcctaagga atatttccat gcgctagtat taggagaaat ggaacgtctc
    10981 catgacgatg agaacggcta tggtccgaaa ggaaaagatt ttattcctca cattgatata
    11041 tcagaggaag tttggtcttc ttacaccaga attaaagagt caataaaaac ggatttttct
    11101 cgtacttgac cattttagca agcgaatcct cctttccaaa taaatttggt tgaaattttc
    11161 aatcgtttat aagataatga ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag
    11221 ataagagaag aggattcaaa tggaaaatca aaataaattg tatcattttg ttggtatcaa
    11281 aggttcagga atgagctcct tggctcttgt tttacatgaa caaggtctaa atgtccaagg
    11341 atcagacatt gaaaaatatt tctttacaca aagagattta gaaaaagcaa atatcacgat
    11401 tctgccattt aacgcagata atgtaaaacc agggatgacg atcattgcag gaaatgcatt
    11461 tcctgattca catgaagaaa tccagcgagc aaaagaatta ggtttggaag tgattcgcta
    11521 tcacgacttt attggtcatt ttatccaaaa ttatacgagt atcgctgtaa caggctctca
    11581 cggaaaaaca agtacgactg gactgctctc tcatgtattg agtggtgtcc gtccaacaag
    11641 ttatctaatt ggagatggaa caggacatgg tgatccgcaa gcggaattct tttcatttga
    11701 ggcctgtgaa tatcgccgtc atttccttgc ctattcacca gactatgcaa tcatgaccaa
    11761 tatcgatttt gatcatccgg attattatac aagtatcgac gatgtattta cagctttcca
    11821 aacaatggct ggacaagtga aaaaagctat ttttgcgtat ggtgacgatg cttatttacg
    11881 caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc acagaaaacg atgatatcca
    11941 agcacgaaac atcgaacgca caacttctgg ttctgctttt gatgtctatc atggggatga
    12001 atttgttggg cacttcactg taccagcatt cggtaaacac aatatcttaa atgctttagg
    12061 tgttattgca gtagcttatt tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct
    12121 gactttccca ggagtaaaac gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt
    12181 cgatgattat gcgcatcacc ctgcagagat caaagcaacg attgatggcg cacgccaaaa
    12241 atatccagat aaagaaataa ttgcagtttt ccaaccccat acgtttacgc gtacgattgc
    12301 attgatggat gaatttgcag aagcacttga tttagctgat aaagtttact tatgcgatat
    12361 ttttggttcg gctcgggaag aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat
    12421 caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct ccattgttag attatcatga
    12481 tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa
    12541 acttttaagc agcacgacga aaaatgtact gtaatacaat tactgcagtg tccttataaa
    12601 aatagaaaga gggggagtgg caaaaatctt gccaagcccc cttttttgca acttatgata
    12661 aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaatcaaa aacatgagaa
    12721 gctgtttttt cttggctgtt cttattattt gatgcaggac agctttttca cgacctctgt
    12781 aaactatagg aatctgttaa atcaagggag cagaacttgc cttttacgct acttttggta
    12841 caataatgta tgattaaaaa taaactggag ggtttctatc ttgaacatag gtaagccgag
    12901 aaaactagga aaaacaataa atgaaatcga ggaaggcgac tcgttatcct taacagaatc
    12961 tattgaagat aaccagcttt tgctgtattt gggattgacg aatgatgcaa atcctttgta
    13021 tatccaacat gactttgcac aaaaaacaga atatcaacag ccaatcgttc catcagtgat
    13081 gctgatgggg atcatcacaa gtgcaatttc aaagcatctt cctggaccag gatcacatgt
    13141 tgtcaatttt tctgtcaatt ttattgaacc ggttttccat tatgaaacac tgacttttca
    13201 gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg acgatttctg tagaagcgga
    13261 gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc
    13321 tgaattggaa atagcaatcg aaagtgaagg tgaattgaat gaataatcca agtacatcaa
    13381 tgtcaggtat caatcgtttt tatgcaaaag tttatggatt tttaggtatg ggacttgccc
    13441 ttagtgcagt cacttcttac ttggctcttt gggtatttcc tttgcaatta gcaactttta
    13501 tcaataattt tccactaggc tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg
    13561 ttctaggcgt aaaagcacaa aagaatccga gcttagcaat cggtggattt attgcctact
    13621 ctcttttgaa tggcataaca ttagcggtta ctcttgcata ctacgatatt ggaacagtaa
    13681 cgcaagcttt cttgacagct actttgatgt tcatggggat gtctgttgtc ggagtaacaa
    13741 caaaaagaga tcttagcggg gttggaagag caggacaggc agctttaatc ggagtgatca
    13801 ttgcactagt cttgaacatc ttcattttga gaagtagcgt agttgagctg ttcatctccc
    13861 tactgttagt cgtcatcttt gcagggatta cagcttatga taaccaaagc atccgtaata
    13921 tctatgtcca gacaaacgga acagcacata gtggaattgc tgtattcatg gcattgaagc
    13981 tgtatttgga ctttatcaac ttgttcttgg cgtttctgag aatctttgga cggaacaact
    14041 aaaaaagaaa aaaattcatc agttggggct ataccgaatt atttggtatg gtcccgcttt
    14101 ttcttttcgc attccgcagg attctttgtt agaatgaata agaactttac agtaaggagt
    14161 taagaagatg acaaaaaata aattattact cgttgatggg aacagtgtgg cttttcgcgc
    14221 attctttgcg cttcacaatt ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa
    14281 tgcaatctat gcattcaata cgatgtttga aaatgttatg caaaaagagc aaccgactca
    14341 tgttctggtt gcgttcgatg caggaaaaac gacttttcgg acagaaatgt atgcggagta
    14401 caaaggtgga agatccaaaa cacctggcga attcaaagaa caaatgccat atatacgtga
    14461 tttgttgaca ggactagggg tacagtatta cgaattgccc aattatgaag cagatgatat
    14521 tatcggtact ttggcagaaa aagtagacaa agatcaattt gatgtcgtcg tgctttcagg
    14581 tgatcgggat ttgactcaat tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg
    14641 tgtcagcgat atcgaaagct atacaccaga acatgtagcg gaaaaatatg atgggttgac
    14701 gccaaaacag attattgaca tgaagggact agccggagat gcctctgaca atatccccgg
    14761 tgtgactaaa atcggtgaaa aaacagctat caagctattg aaacaatatg gctcagtcga
    14821 aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa
    14881 tgacaaagag caggctttct tatcgaaaaa attggcgacc atcaatactt cagcgcctgt
    14941 ggatgtctct atcgactcat tgaagtatga agggaaaaat ctcgataaac tcgttccgtt
    15001 ttataaagaa atggatttca atcagttcct ttctaaattg aatatcgtag aagaagaagt
    15061 aaaaatggat gatattttgt ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac
    15121 ggatatggcg ttgtacgtgg aaatgatggg cgacaattat catacagagg aaatcgtcgg
    15181 agtagcgtgg gggacagaaa agaaaattta tgtcacgaac gatctatctg tattcgaaaa
    15241 ccgagcgttc cattcttgga tcactgatcc gactcggctg aaaaaagtat atgatgcgaa
    15301 acgaacttat gttgctttaa accgctatac tggaaaaacg aaaggaatcg attttgatgt
    15361 cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc
    15421 tgctcattac ggatacaacg atatccaatc agatgaagct gtatacggca aaggggcgaa
    15481 aaaaggttta ccagaagaag aggaactctt ttttgctcat ttagcgcgaa aagtagctgc
    15541 aatcaatgcg ttgacaccaa aattgagcca agaactggca gaaaaaaacc aagaagatct
    15601 tttccggaaa atggaactgc cattgtctat cattttggca gagatggaaa taagcgggat
    15661 caaagtagat gcgactcgct tacaagaaat gaagggcgag ttctctgctc gcttaagaga
    15721 gatcgaacaa aaaatctacg aagaagccgg cgaagaattc aatttgaatt caccaaagca
    15781 attaggagtg atcttatttg aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg
    15841 atattccaca gccgtagatg tactggaaca gcttcgtgaa caagcaccaa tcgtagagga
    15901 tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa
    15961 agtgatccaa tcagatggaa aagtgcatac ccgttatgtc cagacattga cccaaacagg
    16021 acgattaagc tctgttgatc ctaatttgca gaacatcccg attcgattag atgaagggag
    16081 aaagatacgt caggcatttg ttcctagaga aaaagactgg ctgatttatt cttctgacta
    16141 ttcacaaatc gagttacgcg tacttgcgca tatctcaaat gatgaacatt tgaaagctgc
    16201 cttcctagaa ggtcaagata tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa
    16261 ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt
    16321 gtatggcatc tctgattacg gactttcaca aaatctagga atcagccgaa aagcagcaca
    16381 gcaatacatc gatacgtact ttgaaaaata cccaggtgtc aaagaatata tggaaagaat
    16441 cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct
    16501 gccagacatc aattcaagaa attacaatat ccgttctttt gcggaacgaa cagctatcaa
    16561 tacaccaatc caaggaagtg ctgctgatat tttgaaaatc gcgatgatcg aattggataa
    16621 acgattaaaa gaaacaggtc ttcaagctac gatgctgtta caagttcatg atgaacttgt
    16681 cttcgaggta ccggaaaaag aacttgaatc attagataaa ttggtgaagg aagttatgga
    16741 acaagctgtt tctcttcatg taccactgat cactgacagc agttggggaa aaacgtggta
    16801 cgaagcaaaa taaacattgt ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg
    16861 ccagaattac cagaagtaga aaccgttcgt aaaggattgg aacggttagt agtaggaaaa
    16921 acgattcaaa aagttcaggt cttgtggccc aagatcatcg agcaaccaga gacacccatc
    16981 tttgaggctt ctcttgttgg cgaaaccatc caatccattg gacgtagagg gaagttttta
    17041 atttttcatc tcgatcattg tgaactgatc tcacacttgc gaatggaagg aaaatatcaa
    17101 tttacgaaag aaaatacacc aatcgataaa catacgcatg tacttttctt ttttgaggat
    17161 ggaagccaac ttcgctacaa cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa
    17221 ggagcaagtg caacttaccg aggaatcatg aaattgggac ctgagccttt gccagatagc
    17281 ttcttattag cagatttcgc aaatggactg aaaaagtccc acaaagcaat caagccattg
    17341 ctgctagacc aacgtctagt gactggtcta ggtaatattt atgtagatga agcattatgg
    17401 gaagcgaaga tccatccaga acaaccggcg aacacactgc gccccaaaga ggtagaacaa
    17461 ttacgtctat cgatcattga tgttttggat cgtgcaatag aagccggagg taccacgatt
    17521 cgcagttatt tgaatgcatt aggagaatca ggcggatttc aagtagcact gcatgtctat
    17581 cagcaaactg gcaaaccatg tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca
    17641 caaaggggga cgcactattg tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg
    17701 ttttaggttt gacgggtgga atagctaccg gtaaaagtac ggcagccaaa gtctttcagt
    17761 ctcatggatt tcctttgatc gatggcgatg tgattgccag agaaactgtt gagcctggaa
    17821 cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga ggtattgcag cctgatgggc
    17881 aactagatcg agggaaactt gggatgattg tttttccatc aaaggaaaaa cggcaaaaac
    17941 ttgatcagct attagatccg tttatccgaa aagcgataaa agatcaaatc gcctctctaa
    18001 gttccaatca tcctttagta attgtagata tcccattgct ctacgaaggt cattatgacc
    18061 actatatgga tgcagtagct gtcgtttata ctacgccaga aactcagctt cagcgtttga
    18121 tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag aattagtagt caacttccaa
    18181 tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa caatggaaca aaagaaaaac
    18241 ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt tctttgagta tctgtgtcaa
    18301 ttttgctatc ctgagtatat agaaagtgag ggatagcgat gggatcatta aaaaatcaaa
    18361 aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc
    18421 taatcgatga aggtcgtacg caatcagaaa cagcagaaga aaaagaaaag cagtaccaag
    18481 caagaatcga tgaaatagaa gaccagcagg cagaagaaga agcgaagcgg caagatttga
    18541 ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac accgctccca gaaagtgacc
    18601 acattcccga tgagcaaaaa atcgaggaac agcgaatgaa acactataca ggaatcgaag
    18661 agaagttata aaatagtaaa aaatcgagat gtgacttgaa gcagatgaga aggtgttcat
    18721 ctgcttcaag ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt atttctcagt
    18781 aatcaaggtg atcagtattg gaaaattgaa atttagtaag gacaggggac acaattacgc
    18841 atgaaagtag aaaaaattgt tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt
    18901 gatttgtttc gtgttataat acaactagtt gtaaagaaag acacatcctt tatataaaat
    18961 gcgatgcaag aggtgagaca aaatgagatg tcccagatgt caacataata attcacgagt
    19021 aatcgacagc cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa
    19081 ttgttcgttc cgattcacta catttgaacg aattgaagct gctccgttat tagtaatcaa
    19141 gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt ttaagaggac tcatccgttc
    19201 agcagaaaaa agacctgtag ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa
    19261 ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat ctaattggtg aatatgtgat
    19321 ggaagattta gttgatttag acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca
    19381 gttcaaagat atgtctgttt tcttaaaaga gttgcaagat atcgtggata aagcaaaaga
    19441 aaataataaa gagaataata aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg
    19501 aaaatgcttg gaaagagctt caaccaaaaa atatttacca aggaagaaaa gcggctccgc
    19561 tgagagaaga cagcaagaac gccttgctgt atctttatca gccactaatc ggcggagaag
    19621 ccttgagtat ctatttcacc ctgttatcag aaatctctca agaatctggc aaaggtcctg
    19681 aagggatgca tgcagatctg cttagtagtc taggctgcgg tatcccgcaa ttttatgatg
    19741 caagaaaaaa gctagaagga atcggcttac tggatgttta ttttaaagaa gatagtgatc
    19801 tgggacctcg ttttatttat gagctgttag aaccactcat tcctaaagca tttttcaaag
    19861 actctgttct ggcttttctt cttctggaaa aagttggtga acgacgattc cagaagctta
    19921 cccaattctt tgaacctaaa aggcatacag cagagggata tcaaaaaatc acaaaaaaat
    19981 ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa aacatcgaat
    20041 cgctgaataa atcttttgaa gaatctgaat cagtagcatt acttgaagca gattcgacat
    20101 tcgattggca gtttttgaat gattggctgg caaaaaaaca tattgcaagt cttgatgatc
    20161 caatcatcaa acaagtcgac atgtatcatc agctttacgg tttcgatgaa ttgacacttg
    20221 gcgaactgat cagtcaatct tatgacttta gtgaacaaaa agtatctgaa aaagaactgc
    20281 aaaaagccat tttggcaatg cagagacgtc cggtgaagca aacaaaagaa acagttgcgg
    20341 tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc
    20401 tgataaaaga agcacagagc attccaccga tgaaatatct ggaagcaatc aagtcagaaa
    20461 aaggcggata tgtttctaaa caagaaaact ggttactgca agatcttgtt tctcaatcag
    20521 gtctttccag tagcgtgatc aatgttttgc tgaattatgt actagtgatc aagaataatg
    20581 ctagcttaaa tgccagttat gtaaatacag tggcaaatga atgggcacaa aagaaaatcg
    20641 ccacagcaga agaagcgatc cagcacattc ggcaaataag taatcaagca aaacaatcaa
    20701 aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc
    20761 ctgattgggt caaccagcca aaagatgaaa aacagatcag cagcgagaaa aaagcagaga
    20821 ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg tgatatctag tggaagacgt
    20881 aggcaaagaa ttgaagagga tcatcagcaa acgcaattat caagaaaagt atgctgaaat
    20941 gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac accaagaaga
    21001 attgacacaa gcggatattg aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa
    21061 gcgtaaatat caaaaaaacg atccaacgat gatcgctccc ggctatgagc cgaagctgac
    21121 gttgaatttt cattatgtgg atgtcactta tgtaccaacg gaagcattgt tagcacggca
    21181 aagacaagaa gaaatcagaa gccgagtaaa agcaatggat atgccaaaag atattcaatc
    21241 ggcttctttt gaaaactttg aacgtacaga tggacgagga tatgcgagct tagaagccct
    21301 agattttgta gagaaatata ctgctgatcc aaaagggtat cataaaggac tgtatctagt
    21361 aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat
    21421 tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc
    21481 aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat
    21541 gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt
    21601 tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat
    21661 ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc
    21721 gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa
    21781 ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca
    21841 ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc
    21901 agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt
    21961 ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg
    22021 attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt
    22081 gtcggaacga ttgaaagtat cactcaagta gaagcagaag aagaaacgct tcagttgaaa
    22141 ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac
    22201 ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc
    22261 ggtgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg
    22321 caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt
    22381 caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg
    22441 aattttttta ttcctagtct ccttatttta tttggaaaat gctataatta tctagaagga
    22501 gtgaagctat gaaccattta gatttatctc agcgcttagc cgcagtgggt gactttgtcc
    22561 ctgaatccgc tcgtttagca gacattggat cagatcacgc ttacttaccc gttgcattga
    22621 tattaagagg aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat
    22681 cagcacgtaa gcaagtcaaa aaagatggat tagaagaccg tatcaccgta cgccttgcaa
    22741 atggtctaga tgctatccag ccacaagatg gtattacagc tgtcacgatt gcgggtatgg
    22801 gaggttcact gatccgagat atcctagaag ctggcaagtt gaatcaacgc ttaagcggac
    22861 aagaacgttt gatccttcag ccaaacatcg gagagcgtac attacgatct tggctgcaag
    22921 aaaatcagta taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa
    22981 tcattgtagc cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag ctgatgttcg
    23041 ggccatttct gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca agagagcttc
    23101 aacaaagaaa agctatccgt gaacagctca aacaagcagc taatcaagag gcacgtcaaa
    23161 aacaaatcga gcaggaaatc caaatgattg aggaggtttt aaagccatga caatcagcgg
    23221 acgcactttc atccaaaagt ttgaaagcta ttgtcctcag tggctagctg aagaaggaga
    23281 cccagtcgga ctgcatatcg gaacattgga taagccaatc caacgagtaa tgatgacatt
    23341 agacgttcgt ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc
    23401 taaacatcct ccaatctttc gtcctgtaaa acggctggtt actgatcagc cacaagaaaa
    23461 aatgtatgca gatttgttga aacatgatat tgccgtctat gcagcacaca cgaacatgga
    23521 tattatttgg gatggactga atgactggtt ttgtgaactt ctcggtattg aagtggagag
    23581 ttatttagta aagactcatg agattcatta taaaaaactg gccgtttatg tacctgtgga
    23641 tcatgcacag aaaatgcgtg aagtattagc tgcagctggt gctggtacac aaggtgatta
    23701 taccggtaca agttttacct cgattggaca cggtcgattt actccggaag ctggtgcaca
    23761 accagcgatt gggaaagtag ggaagacaga acaagttcaa gaggcaaaag ttgaggttat
    23821 tttgccggaa actatcgaaa aacaagtgat tcaagccatg cggtcagctc atccttatga
    23881 ggaacctgct tatgatcttt tcgctataga tgaaccagtc gaaatgtttg ggttaggacg
    23941 agtaggtgaa ttgccgcaag aaatttctat agaggcattt gtagaacaag taaaagaagc
    24001 gttccaacta gatggactga gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg
    24061 cattgcgatt tgcggtggaa gcggtgaaaa attttatccg caagcaatcg cccaaagagc
    24121 agatgtctat atcacgggag atatctatta tcatacggct catgatatgc aaagcgctgg
    24181 gttgattgca attgatcccg gtcattatat tgaatcgtta tgcaagcaac gatttatcga
    24241 gaaatttgaa tcttggaaac aagaagaaaa ttgggatatt aactttttcg tttctgaaac
    24301 aaatacaaac ccatttcaat ttaattaaaa ggagagaata ctatgtacga aaatcttttg
    24361 ccacgttttt tacgatatgt caaaacagaa acacgctccg atgcaacaag caaaacgact
    24421 ccatcgactc agacacaagt tgcttttgcg caagaattga aaaaagaatt agaagagtta
    24481 ggcttagctg atgttttcta taatgaagat aacggattcg ttatgggaac attaccaagc
    24541 aatgttgatc atgaagtgcg ttccattgga tttatttccc acatggatac agcagatttt
    24601 aatgcagtag gagtcaatcc acaaatcatt gaaaactatg atggcgagtc tacgatcgta
    24661 ctagatgcgg aagaacgcta tacattgaat acaaaagatt ttccaaattt gaaaaattat
    24721 gaaggccaca cattgatcac aacagatggc agtacattac tgggagcaga tgacaaatca
    24781 gggattgcgg aaatcatgac tgcaatggag atcttgatca ataatccttc tatccctcac
    24841 ggtaccatca aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc ggataaattc
    24901 gatgttgagc aattcgatgt agattttgct tatacagtcg atggtggtcc tttaggtgag
    24961 ttgcagtatg aaacgtttag tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta
    25021 catccaggga cggcaaaaga tacaatgatc aatgctctac agttagcaat cgattttcat
    25081 aatcagcttc cagccgatga agttccagaa aaaacggaag gatacgaagg tttcttccat
    25141 ttgatggcat tggatgggaa tcctgaagaa gcttctatga gttatatcat tcgcgatcat
    25201 gatcgtgaaa aattcgaagc acgcaaagca caaatcacac atatccaaga agagttgaat
    25261 cagcgatttg atcaagaacg aatcaaagta gatatgtatg atcaatatta caacatgaaa
    25321 gaaatcattg aaaaagatat gagcatcgtg gaattagcga aacaagcaat gatcgagctt
    25381 ggtatctctc cagtgatcga accagttcgt ggaggaacag atggttcaaa aatctcttac
    25441 atgggtattc cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg acgattcgaa
    25501 tttgtttctt tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg
    25561 aacacaaaga gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg
    25621 gaatgtcatc tgtctaaggc taaagggcag gacaatggaa ttggttgtcc cgccctcttt
    25681 tttgaagagt aaataggagg taaagaatgg aaaagctgct agttttttat aacaaaagtt
    25741 cagggaaaga tgagggtgag aaattagcac gctggtttca agattatgta aaagaaaagc
    25801 agccagaatt atatgtagaa ctagctgaaa ctggcccatc gattgatgat gcggaattag
    25861 tcaagaaagc agaagccata gaagctgata ctttagtaat tataggtgga gacggcacga
    25921 ttcatcatat cgtccaagcg ttccaaaaaa cattatctca ataccatgtc ggattacttc
    25981 caggtggaac tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag
    26041 ctgctgacgt gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg
    26101 aagtcattat ctctacactg acaatcggaa tcttagcaga tacagcagtt tggatcagtc
    26161 aagaagaaaa acagcgttac ggctcgtgga tctttatcaa gaagtttttt aagttattgc
    26221 tgaagaagaa aaaatatcat ttggatttag aaacagacga ggagcattgg aaaggaaaat
    26281 cccaactgtt gacagtcacg atgtctaact ccgtgggcgg ttttacaaat tttgacgact
    26341 cagcgaccct agatgatgga aaatttcatg taacgatcgt accttcctta gatattttcc
    26401 gatttatttt ttatttgcct agaatcatga gaggcaaatt ctatacgatc cccggcatta
    26461 cttatttcac agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa
    26521 cggatggaga cacgacagat gatcttccag ttgaattatc tgttgtatca aaaggattaa
    26581 cagtttattc accaaagaaa gaagaaaaag cttagaaata gtagttaaca gaaagttgga
    26641 agaagagtaa tggatgtatt gttacacaat cctttgtggt tttacctttt cattgtgaat
    26701 ttttacttat tttgtctgat ggggtatgat aaacatcagg ctagaactag cggctggcgt
    26761 gttcctgaga ataatttgat atttgtcagc ctgttaggcg gtggtttggg aggattgatc
    26821 tctcgaaaag ttttccatca taaaactcgg aaaaagaagt ttactatagg attcgctgca
    26881 ggtgtacttg ttgatttgat tctcatttat tttttccatt aaggagcagg agatgtcgtc
    26941 taaaggaacg aaaactaaaa tcattctgaa tctgcttttg ctgattgtga tttttgccat
    27001 tatttattat ttaatcaatc aatcatttgc agatatcttt gccgaattgc tgtctaccag
    27061 tgttgttgtt ttggcagcgg ctgttatatt agggacagtt taccaaatcg ctgaagggcg
    27121 ctcaatcaaa gaaattgctg aacctttcgc gccgaatttt acaacgaaag atgggttctt
    27181 gacctctagc tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc
    27241 ggagatttat ttttataaca aaaaaggaat gaagctatcc cagggagcag gggtgacagc
    27301 actccatatg attatgtaca aactggcagt gatcacttat gcattagctg gcttggtcat
    27361 ccaattctct cttttttatt ctaatggtcc atcaatgatc tggtttatca tcgctggtat
    27421 ccttttgacg atcgtgattg ttgccttttt attggcgatg tccgtcagct tgaatgttca
    27481 aatatttttt gtcagcttat cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt
    27541 ggatacatgc aatcagcaga tctactcttt aagggaaacg gtccaaacca ttatccaaga
    27601 caggactgcg ctattgcgga tttatctatg gaatatgtta aaacttgtat tttggtatgt
    27661 acttccttat ttgattctga ttgaaaacca tcctaaaata gattttctac tgaccatttc
    27721 gtttatcagc tttgcagtag tgctttcagg ggttattcct acgcctgctg gtattggttc
    27781 ctttgaattt gtctatttgt tattattcag accagttgtt ggaacagttg acgcagtttc
    27841 ttcgctttta ctgtatcgtt tcagttcctt tatcttgcca tttctctatg gatttgtgta
    27901 tgtgattgct gaacggcgaa gagtgatcaa agaagaaata atagaagtaa aaaaagagaa
    27961 taaagaaaat caagggtagg tcttttataa gatctaccct ttttatcaaa tcaaagaaaa
    28021 acgttatgga taagataatt tta
//
LOCUS       DAZKCN010000027        27596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000027 DAZKCN010000000
VERSION     DAZKCN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.027"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..398)
                     /locus_tag="ACG8K3_001100"
                     /pseudo
     CDS             complement(1..398)
                     /locus_tag="ACG8K3_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            477..653
                     /locus_tag="ACG8K3_001101"
                     /pseudo
     CDS             477..653
                     /locus_tag="ACG8K3_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(829..1398)
                     /locus_tag="ACG8K3_001102"
     CDS             complement(829..1398)
                     /locus_tag="ACG8K3_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9144996.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1472..2761)
                     /locus_tag="ACG8K3_001103"
     CDS             complement(1472..2761)
                     /locus_tag="ACG8K3_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144997.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3088..3963
                     /locus_tag="ACG8K3_001104"
     CDS             3088..3963
                     /locus_tag="ACG8K3_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9144998.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            3977..4300
                     /locus_tag="ACG8K3_001105"
     CDS             3977..4300
                     /locus_tag="ACG8K3_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9144999.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4297..5049
                     /locus_tag="ACG8K3_001106"
     CDS             4297..5049
                     /locus_tag="ACG8K3_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP9145000.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5039..7210
                     /locus_tag="ACG8K3_001107"
                     /pseudo
     CDS             5039..7210
                     /locus_tag="ACG8K3_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7322..9493)
                     /gene="copB"
                     /locus_tag="ACG8K3_001108"
     CDS             complement(7322..9493)
                     /gene="copB"
                     /locus_tag="ACG8K3_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP9145001.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9513..11699)
                     /locus_tag="ACG8K3_001109"
     CDS             complement(9513..11699)
                     /locus_tag="ACG8K3_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9145002.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11699..11908)
                     /gene="copZ"
                     /locus_tag="ACG8K3_001110"
     CDS             complement(11699..11908)
                     /gene="copZ"
                     /locus_tag="ACG8K3_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP9145003.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11921..12361)
                     /locus_tag="ACG8K3_001111"
     CDS             complement(11921..12361)
                     /locus_tag="ACG8K3_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP9145004.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12435..12974)
                     /locus_tag="ACG8K3_001112"
     CDS             complement(12435..12974)
                     /locus_tag="ACG8K3_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFP9145005.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13589..15055)
                     /locus_tag="ACG8K3_001113"
     CDS             complement(13589..15055)
                     /locus_tag="ACG8K3_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9145006.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15366..17447)
                     /locus_tag="ACG8K3_001114"
     CDS             complement(15366..17447)
                     /locus_tag="ACG8K3_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9145007.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17589..17711)
                     /locus_tag="ACG8K3_001115"
     CDS             complement(17589..17711)
                     /locus_tag="ACG8K3_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145008.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            complement(17971..18330)
                     /locus_tag="ACG8K3_001116"
     CDS             complement(17971..18330)
                     /locus_tag="ACG8K3_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9145009.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18360..18701)
                     /locus_tag="ACG8K3_001117"
     CDS             complement(18360..18701)
                     /locus_tag="ACG8K3_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145010.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(18698..19372)
                     /locus_tag="ACG8K3_001118"
     CDS             complement(18698..19372)
                     /locus_tag="ACG8K3_001118"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9145011.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20425..21723)
                     /gene="asnS"
                     /locus_tag="ACG8K3_001119"
     CDS             complement(20425..21723)
                     /gene="asnS"
                     /locus_tag="ACG8K3_001119"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9145012.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21763..22953)
                     /locus_tag="ACG8K3_001120"
     CDS             complement(21763..22953)
                     /locus_tag="ACG8K3_001120"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9145013.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22974..23501)
                     /locus_tag="ACG8K3_001121"
     CDS             complement(22974..23501)
                     /locus_tag="ACG8K3_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP9145014.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23548..26337)
                     /locus_tag="ACG8K3_001122"
     CDS             complement(23548..26337)
                     /locus_tag="ACG8K3_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP9145015.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26484..26684)
                     /locus_tag="ACG8K3_001123"
     CDS             complement(26484..26684)
                     /locus_tag="ACG8K3_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9145016.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27136..27456)
                     /locus_tag="ACG8K3_001124"
     CDS             complement(27136..27456)
                     /locus_tag="ACG8K3_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145017.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7753 a         5856 c         4850 g         9137 t
ORIGIN      
        1 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc
       61 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
      121 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc
      181 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg tgttacccat
      241 aaatgcgatt tgattcctgg ttggttataa ggagtgtcta atgttaatgg aataccatat
      301 ttcattgctg aatctcttgc caattcaacg tatactttat agttcttttc aaaagtttct
      361 ttatcatgtg tgtgttgtaa ctcgatttgc acaggacttt tggctcacga ggttgtccta
      421 ctctacatct tgctgataga tatgctgaaa ggaaaacacc aacattatgt tttgcttttt
      481 cagctagaac gagacaagca ggaatacctt tagaagatat caaagatttt ttaggtcata
      541 aagatgtttc cactacacaa gtctatgccc atatttctcc cgaagtgaaa aaaagatcaa
      601 tgaatcaact agaaaactat atagaagagc aaataaaaaa gcactcaaat tgagtgcttt
      661 tttaagctca ctatcactaa aactatcact aattttattt ataagcgaac atgtttctat
      721 cactgatttt ttaaatctag cgaaaaaccc ttatcttaaa ggcgttcgtt caattctttg
      781 gcaaggtctt caaagcctgg cttgcctaga agtgcgaaca tggttttctt atagctcatg
      841 tagaaaaatt ttcttgatga tgattttatc catcaattga gattgactgt tttgctctcc
      901 tatgatttca gctatgattg tccgattttg cggttttctt cttaactggt agttgatttt
      961 cccagtttcc atcaactcgc taagcatatc cacgactccg ccaaatccgg tctttttcgt
     1021 aataaaacct agtgctacca ttcgttcatc tagatttttt gctgtaatcg tcacaccagt
     1081 aatgagcgag tgctgtaatg tttgggccag ctcttccatc ccttcagcat tggatacgac
     1141 ggaagctgtt ttgttaatag gttcttcgtt tatttctttt ggttctttcc tctccacttt
     1201 cactgtttga cgaaggacat ctagttcttg caagagcgtt ttgtattgtt cttgggtcag
     1261 catcacgcca gctacttttt cacgattaaa tatatatacg ccagttcccg cgtctcgcgc
     1321 ttgagcaaat acatccatcg gcgatcgttt cacttctgtg atcgaagatg tgggaacttc
     1381 taatttcttt aattccaatg gatttcctcc tttactaatc gctttttatt atagcacaga
     1441 ctgacttgtt ttgatctttt ttccaaaggt tttatcggtt tttgcctaac cagtaattga
     1501 tatcggtaag aagtctttcg ataccaaatg aaggatggta cagatacgtt ttttgatctt
     1561 cgttatggtc tagtggaaaa aaatgatcgg tgatcactag atctgcatac cgaggcggct
     1621 tatttcgagt acatacatgt atagagatcc gttttcgatg tcttagcgtt ttgttcagta
     1681 ccccagcaag tcttcttgct tcttggtgtt catagcgaaa aaaaaagacg attttcactt
     1741 caggagggtg gacatctaaa taccctcgtt cctggagtgt tttcagtaaa agcatccccc
     1801 actctactgc ggggtgtgca agtgcttgtt ggatcgctag ccgaaattga tatagtttat
     1861 ctttggaatt gggaaatttt tctaatgttt cctgataata acgaacttct tctatcagcc
     1921 ggtcatggat accaattacg taacgaagcc attcttggta aagtactttt atcagctttg
     1981 atttgctttc gctctcaaat tcgctattca gccaaaacag cgaatcaagc ttttcagtca
     2041 caatcgtcaa tttccgatag tcttgtgtct cttctatccg tttccaccgc ttttcagtaa
     2101 tctggttaca gctcaagcgc aaaattgcta gataattttt ttcttttatt ggcaaaatct
     2161 gctcctctct aaatagcaat tcaggaaaca attcaaagaa tagtgttcga ctgtctccta
     2221 aaatctcgat ttctgccgga tcctttcttt gcttcaataa ttggatgaac atctcagaaa
     2281 aggactgctc tccatcatct gcaaaaaaat gcggaaaatc tttcaaaaag taaaacagca
     2341 gatatcttac ttttctttct gaaccaaaga tctgtgcttt tttataagaa aaagaaaggt
     2401 ggtactcgcc aaaccacttt ctcaatcgct cttgcgtaga acgatcgtac ttctgctcgt
     2461 aaggttcatt taatagcaga tttttcagta aacaaaacgg gggtgattgg atcagaaaaa
     2521 caaacaagag ataaggttcg ctatatcctt gatcataaac taactgaacg gttttcttat
     2581 gaagaatcag ctgaatggga cagtcgcttt ccgccaaacg tttcagatgc cgatccactg
     2641 ttttaacatc gattttcagt tcttcagcta aatcagcttt tcgccagatc aaatctttca
     2701 ttagattgat tagtgaaaat aaattctgtg cattttttga taatagtttt tgccatctca
     2761 tcgatcattt ctcacctgat ttctaaacat attgtttgat caaaatgagt tcactcctaa
     2821 aaagataaaa aactcccttt ctatttattt aaatacgcaa atcttcctca ttatttttta
     2881 ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa aaacataata
     2941 agatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat agcgatttta
     3001 tgagaaggtg atgtgaaaat ggaagaaaaa atagaagaat ccaaacaaaa aatcgatcag
     3061 ttgatataag aattagaaaa agcggtcatc ggcaaacgtt caactatcca attgatgatc
     3121 gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt aggtaaaacg
     3181 ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt ccaatgtact
     3241 cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca atcaaaagag
     3301 tttgaatttc gtccgggacc gatctttacc acgatcttgt tggcagatga gatcaatcgt
     3361 acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca tgcaacgatc
     3421 gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca aaatccaatc
     3481 gagtacgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt attccgacta
     3541 caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttggataa attcgaaaaa
     3601 gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat aaaaacaaat
     3661 gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt agtgtcagca
     3721 acaagaagtc atgctgccat ccagcttggc atcagtccac gaggaagcct ttcctttatt
     3781 caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc caaagatttg
     3841 caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg ttcattgaag
     3901 gaagacgaaa agaaacaaat cattcatact cttgttgcac aaattcctat tcctgtgagg
     3961 tagattgagg tagacaatgg aaaaaatcaa agattatttc attaattttg tgttatttct
     4021 ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag gctggttctt
     4081 gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca gctttctaaa
     4141 gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa gtatgcaggt
     4201 tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt tacaaatcgg
     4261 cttcccagca atctttgcag aaaaaaacaa gagattttga ttcttacaaa taaaccgcaa
     4321 gaagtccaat tgctttggca gccaaaagaa agaggatgcc atgaaacatt aactgtcatt
     4381 tttacagcct atgatagtct aggacttttc agaaaaagtg caaaaagaga gatctctttt
     4441 cctgtgactg ttctgcctgc tttttgtaag attcaagcag aaaaattaca gagagttctg
     4501 gaaaaaaaac agcagctgaa cgcgtaccaa agaggtcttg attttcggga acatcgcgct
     4561 tatcgtccag gtgattcttt caaccgaatc gattggaaat tgtctgctca tactcaagag
     4621 tggttatacc gtgaatacga ttatcaagaa gaagaatgtt ctcctctttt ttgcttttgg
     4681 ggttcgccca gtccaaaatt tgaacctctg ctagacttgt atttttcttt atggcatttg
     4741 agaaaagaaa agcagccgga acttctgata ttaggcaacc gcgcctttca tggcaaagac
     4801 cccggtcata cctattttgc ttctttggaa gctggcgatg agaaagcttt tttcgcacac
     4861 ttgaaaaaat atgcaaaaaa acaaatcgtc ctctttgttc ctgagaattc tcctgaagtc
     4921 tcagaagcta ttgaaacatt gtcaaaagat cgaacggctt atcttgttta tttcgtagaa
     4981 aaacaactca tggtccaaga aaaagacaag atatgttcat tgccaggagg tgaatggatt
     5041 gacgattaaa cgggaaaaat ggctgcgtgg cgtttactcc tttgtgatac taagtgtagg
     5101 tgctccgata tttgtagtag ctaatcaatt gcaaaacagc taccttctta tcctaactat
     5161 tggtgtccta tgccttattg tctgctgtat tcctaaaaaa tgggtacgtt tgctgctttc
     5221 tttgccagta atgataggtt gtttgttttt atattttcct tcgaagacat tttccatatt
     5281 atggatctta cagttttttt cgtcagttac agaggaattc ttccatctat cccaaggtaa
     5341 actttattat ttgccagaaa agacagcttt ttttctcata atgttgctga tctatttatt
     5401 tattacattg accgtggatt acgactattg gtacgcaccc tttctttctt tgatggctta
     5461 cttcatgatg cttaccgttt ttcggaaaaa agatttactg tacgaaatga ttgcggtagt
     5521 aactgttttg ttcgtgtatc tggcagcaag gcaatttttc tccatccggt tgttgtcggg
     5581 aaacactcga ttccagtcat tgactacagt tctgcttatt tgtttcttaa cttttagcgc
     5641 tatcttgcct ttatatcttc cgaaagtaca ccaatctctc gaggaaacga gtgaaccgat
     5701 ccgagaatat cttaatgatg aagggcttta tgacacactc catgagtatc aattaaccgg
     5761 aagtgcaaga acaggcttca gtgagaatga tgaacagctt ggtggacctt tatatgataa
     5821 tggcgaagtt gtttttacag cgaaccaaaa aggaaatggc tattggaaga tcgaaaataa
     5881 aaatcattac accggttctg gatggataga agatcaggac gcacccatcc aaccaatcca
     5941 agaattccct gttgacatca cgagctacca aaccgttata gaagacaaag aaaacattac
     6001 attgacccta aaaaaacaag ctttcttacc ttatccttat ggaaatacta ccttgactga
     6061 tttttcatta gctagtggac aaacgcttta ctatttgcct gaaagccagc gattcattcc
     6121 tgtcccgaat gaatcgacac gttctgcaac tttacaaaca gaaaggttag tttttacgag
     6181 agaagaatta aaaagcagtc agttaaaagc tgatgattta gaaaatcagc agacaaatct
     6241 tcaattgcca aaaagtttgc ctaaacgtat tgaggacttg gcggtcgaat tgaccaaaaa
     6301 tgaagcaact ttaattcgta aagtcgaagc tgtctaacag tatttaaaaa caagcggaaa
     6361 tttccgctat tccaaaacgg ataccgggca tactccgaaa gaacacgatt atgtggatta
     6421 tttcctcttt gattcaaaag tcggctattg tgacaacttt tcaacttcta tggtcgtcct
     6481 tcttcgaaca cttggtatcc cagctcgctg gacaaaaggt tttacaaatg ggctcagaac
     6541 aggcacagat aatcaaggga atgaggaata cagcattctc aatagtcatg ctcattcttg
     6601 gccagaggta tactttcctg gatttggctg gcttccattt gaaccaaccc cttcatttgc
     6661 caatccaatg ggttcacaac cagttgctga aacaaccgaa accgtccagc ctgctatcga
     6721 acaaccaaat gaaagcgaaa atatggcaga aactatttcc agtgagagat ctgaggtcat
     6781 tccatcagaa gaaacaacac tcacaccagc ttataaaatc gtcttattca gccttggagt
     6841 gtgtttatcc gtcttgttgt tctttctatt gttattcaga aaatctctgc gctggacaag
     6901 ttatcttatc tatcttcatt tttttgcttc tactaatacg attgcatatc aaaaacttct
     6961 taaccctttg gaaacattgg taccgcgcaa acccgcagaa tctttgcagg aatatagcct
     7021 gcgaatagaa caagaattca agctagatct gcgtttcatt cgattaacta ctcaatatga
     7081 agcagaaata tatggacgac aagcagatcc ggtaaaattc gataaaaaaa cctatttaga
     7141 aataacgcgt ataataagaa aaacggtata caaaaaaatt ctgacaaaag ggagccgctt
     7201 caaaaaataa gataaaattt ctggagaatc tacttctgga atgccgagaa ccagctgatt
     7261 gagtatcgat tactcgaaaa aaaggttgtg acacgttttt tgtcacaacc ttttttcatt
     7321 attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt aacgacatca
     7381 agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt ccggctgcta
     7441 gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc attttctttc
     7501 gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg tttgtcaata
     7561 cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc ccgattgaag
     7621 cacgggctaa acttggcgca tcattgatcc catcacccac catgattact tttttccctt
     7681 ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg ccataatatt
     7741 cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca ccagtaagca
     7801 tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct tctgttttta
     7861 ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg aagctgatgg
     7921 tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat ccgagacgct
     7981 caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc tctagcccga
     8041 ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt tctttcaaat
     8101 aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct cctagatatt
     8161 gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt acagtaaatt
     8221 ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca ttggcttgct
     8281 ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat gtggagcgag
     8341 caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata aagactgtta
     8401 ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg aatgccagga
     8461 tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat aatgactcta
     8521 gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct agataaccat
     8581 tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata gaaccgccaa
     8641 tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca gctgattcgt
     8701 caacaattgt ttcacctttc aagattacac catctgttgg cattttatca ccagaccgga
     8761 cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct tttccatctt
     8821 tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa gcattgctgg
     8881 cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg atcaaagtag
     8941 ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt gcgataaacg
     9001 aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc atcatagcag
     9061 gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg ccatagataa
     9121 atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt acttgaaatg
     9181 gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg ataagcgata
     9241 gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg ccgtgttcgt
     9301 gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca tggttatgtc
     9361 ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc tgtttttcat
     9421 gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt ttctccatat
     9481 cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg atttaagctc
     9541 agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc aatgattgga
     9601 ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa agcccagaaa
     9661 agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg tgcaatggat
     9721 gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc cgttccactt
     9781 cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat tccatcgcct
     9841 gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc tttatcctct
     9901 ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc aatcgtttcg
     9961 gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt ctggagctgc
    10021 ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca cccgatcact
    10081 tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc taacgcgtgt
    10141 tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt acccgcaaaa
    10201 tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt aaaatcaatc
    10261 atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt acctagtgga
    10321 tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga accgactaga
    10381 tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac aatgctgtct
    10441 aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt tcgtgcacca
    10501 acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca tggacacgca
    10561 atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt gatcaaacct
    10621 gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat ccctgagatc
    10681 cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac catttgaatg
    10741 atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag cagaccattc
    10801 gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg aagattttca
    10861 ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc atctgctgga
    10921 atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc aatcgctaac
    10981 gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga catcaattgt
    11041 ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc gagcaaaatc
    11101 agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc acggaaaaat
    11161 ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa tacatccata
    11221 tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc accgacataa
    11281 aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa gtgaacaatc
    11341 gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa agtcaaaacc
    11401 gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc ttctgcaatt
    11461 ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc tacgctttgg
    11521 attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt ggccaagttg
    11581 actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct agcagaacaa
    11641 tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc tgttgccatc
    11701 aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt tcggattgaa
    11761 cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact ttcacttttt
    11821 gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga ttgcagctca
    11881 taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt tgtttacatt
    11941 cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt tgttccagtt
    12001 gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact agatccgcaa
    12061 tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc gcactgcgaa
    12121 ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct tgttctgtcc
    12181 agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct ggtttccaat
    12241 ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca agtgtccaaa
    12301 taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg actttttcca
    12361 tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca gattacgctt
    12421 gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa aaattcttgt
    12481 ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac ttggatcagc
    12541 tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa aaattctaag
    12601 tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga ttttttgttt
    12661 atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg cggcacttta
    12721 agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt ataatttcct
    12781 tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa aaaccacttt
    12841 tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag tagcggaaag
    12901 tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact ttgtccaatc
    12961 acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat cataccactt
    13021 ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc cttgtttcga
    13081 ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca aaatatgagg
    13141 agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt acaacctctt
    13201 ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc aacaaacatg
    13261 agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg ctcactacct
    13321 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca aaacgaaaaa
    13381 tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca
    13441 acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga aacttcacaa
    13501 aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc
    13561 acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct ggttcagtga
    13621 tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat aagatcaaga
    13681 caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga tcagcagtat
    13741 aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca aagcaccaag
    13801 caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa ttgaatttct
    13861 tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc attggcatga
    13921 caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg ataccaaaga
    13981 tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca ttcaccaatg
    14041 tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc gcatctgcta
    14101 acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt ccaatcatgt
    14161 ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta cctaaaattt
    14221 gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg ctagcaaaca
    14281 gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct gctaagaaga
    14341 tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa ggggatattt
    14401 tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa ctgaaatcaa
    14461 atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc atcgttggat
    14521 tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg aacatcgctg
    14581 ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat aaatataaaa
    14641 aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc gtatttacga
    14701 tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga ggcttttgtg
    14761 ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc agtcgtttat
    14821 tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg aaggctgacc
    14881 ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc aatagccaag
    14941 aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc atgccccaaa
    15001 ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg ttcattcgtt
    15061 ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt ttaacaaact
    15121 tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg ttttttcata
    15181 ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat tgaatttcaa
    15241 gctattttga attttctgca aaattaaaaa cattagttat tttataatcc tatctatttt
    15301 ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt cgtcctcttc
    15361 attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac agctaatact
    15421 gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa agcacctaag
    15481 gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac gattttcaat
    15541 gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt catgatgact
    15601 acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat tcctacatct
    15661 gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt ctttttattg
    15721 tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa ttcgctatat
    15781 atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga atcgcccgtc
    15841 aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc tacggcatct
    15901 tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat cgctagataa
    15961 agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc catcaatttg
    16021 tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc acctgcaaat
    16081 tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac agattgagca
    16141 atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat ctttggatcg
    16201 atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc ggttttatcg
    16261 aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt tactaataca
    16321 ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga gatcactaat
    16381 gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc agaaaacgcc
    16441 gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc tacaacgact
    16501 ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt tttactgctg
    16561 gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga tttttctact
    16621 tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc accaacatat
    16681 acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga ttctccttcg
    16741 atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa ctgaccgatc
    16801 tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg agctgtttct
    16861 ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc caatccttgg
    16921 aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata ttcaccgatc
    16981 aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat ttttcctttg
    17041 acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata aatcaataaa
    17101 taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc gatacctgca
    17161 agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg ttcagatact
    17221 ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac tacttttttt
    17281 atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag ttttccagaa
    17341 gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt ttcgattttc
    17401 attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt aatgacctcc
    17461 tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt attcatatgt
    17521 caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt ccatacagat
    17581 gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat ccaacccgca
    17641 cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc tggcaatttg
    17701 tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc aaaatgtcct
    17761 tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc tttaatgaaa
    17821 attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa aagtcacatg
    17881 gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc cacctgactc
    17941 tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga atggcatcaa
    18001 cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga tcgtgataca
    18061 tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa cgatcagcca
    18121 gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt tgccaaaagg
    18181 cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac ctatgatcgt
    18241 gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc cgccgaatat
    18301 aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac tgtttttgat
    18361 taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat caaatattct
    18421 gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg ttggattagt
    18481 tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc ttgcgcaaat
    18541 ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt atcatttagg
    18601 tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag ctgattgatt
    18661 tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt ttctttcgct
    18721 catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag ctttgtgcaa
    18781 tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat ttcgaaaaac
    18841 cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat gcgccatatt
    18901 ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc cagccatgac
    18961 ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag attcccactg
    19021 tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca gatttctcag
    19081 ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg atcaaaatcg
    19141 atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc agatctgcaa
    19201 ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga tattgggaga
    19261 ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct tctgctcggt
    19321 ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc atttgtcaaa
    19381 aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa gccctatcaa
    19441 gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc aaacaattga
    19501 atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt gccggattcc
    19561 ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt ccgtcaatca
    19621 gaccactgat gacgccatca gaacatatat ttataggctc atctaagact tctagtaaat
    19681 gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa ctttatctgg
    19741 tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct gaaaatacgg
    19801 acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat gctgctttat
    19861 ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct ttgtaaatgt
    19921 ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt cttttgcttt
    19981 cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt
    20041 tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc acaagtcaca
    20101 atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc ggagctgaac
    20161 ggcaggcgca caacctcttc cttaaacggt gttcaaaagc cagtttctcg gtattaagcc
    20221 aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt tttcttaata ctcgaaacta
    20281 aacagctttt tcacaacctc tccactaaac ggtattcaaa agcttacatc tgcggtaata
    20341 agtcccgcca attcaaaaac atgaacagct gtttttgctt ggctgttcta attacttgat
    20401 gcagaacagc tttttcatca cctcttatgg ataaattcgg ttcaacaaac gtgggaatgg
    20461 aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt caagacctag
    20521 accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc atgaatattc
    20581 tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt ctgttgcccg
    20641 ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg cacagatcac
    20701 cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt ttggatagtt
    20761 taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag gtgaaccaaa
    20821 gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga tcgcatcatc
    20881 ataagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt tgtctcgtcc
    20941 tagtacatct aatgcatatt cgcaatgatc taaaacattt tgtactaaga atgctacata
    21001 ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg gttcgatcat
    21061 ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag tcggaccgaa
    21121 tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc cactttgaga
    21181 taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc cttctggtgc
    21241 agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa agaattcata
    21301 cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg aagaacgcag
    21361 ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg tgatcggata
    21421 atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac caaattttga
    21481 acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt gtagattttt
    21541 agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt ggaaaaaggc
    21601 tgttccgtct cttaattgta aaaatgcgat ttttccgctt gatcgcttat ttgcgaccca
    21661 agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt gaatttgttc
    21721 cacgtatgac tctcctttta tattccttga atttctttag gattaatttt gacttttact
    21781 ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat cagtagcata
    21841 actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg ctacccctgt
    21901 ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca tgcgtagcgt
    21961 ttcttttaca ttaggaaaaa gataaaaagc tccttgtggc ttttcaagag aaacacccgg
    22021 cagttctgcc acaagcgaat ataacgtatt caggcgttct tcaaaggcgt tccgcatttc
    22081 ttccactgta tcttgctcgc ctttcaatgc ttcaacagct gcatattgac tgactgctgt
    22141 cggattactg gttgactggg aagcaacagc gatcattcca ttgatgatca caggatttcc
    22201 caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc cgttgatgat
    22261 gatagtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact cattgccatt
    22321 ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt ttactgccca
    22381 ttcaccgatt gcctgcagtt cattcttgct ataaatcatt ccagtcggat tcgatggaga
    22441 attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt caacagtaac
    22501 tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg ccagttttac
    22561 ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt gcgggtcaag
    22621 gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga ctgtttgtga
    22681 aggttcatag acaagtccat agtttttctt cagataatca acgattgcct gtcgaagctc
    22741 tgggattcct gctgttggtg tataatagct agctttcccg ttgcggattg cttcgattgc
    22801 tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca aactcagcac
    22861 atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag tgacagacgg
    22921 ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac cttttaaatt
    22981 cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata attcagtttc
    23041 ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat ccctaaagca
    23101 gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc ttcgttttca
    23161 gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg aatgataacg
    23221 gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt ctttcgagta
    23281 aaccaataaa aatgatcggc ttcctcaaga tttgcatatt tctttgcgat ttctactgct
    23341 tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga gctgatcacg
    23401 atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt tttttccttt
    23461 gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca ttatagcaga
    23521 ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct ttaagaattc
    23581 atggatgtcc tgatagattt cctccatagc tagctctttg atcggtactt ttttcggtaa
    23641 tgcatggatg atccgtgcac catattttgc agtgactagt cttcgatcca aaataagcaa
    23701 tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta acttcaaagc
    23761 tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta aataagcgtt
    23821 cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga ctacaacgat
    23881 ctgcaaagca tccccaggca aatctacacc ttcccaaaag ctgtcagcgc caaacaatac
    23941 gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccgc caaacccttg
    24001 agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat agaccttctg
    24061 caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt cgatcagacc
    24121 tgtaagtgct gtctctaaat aatcgatata tgcttgattg ctgacttgat ggacggcagg
    24181 tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag aggcaatcac
    24241 tttaagaggt gctgcttcaa tgccaagccg cttagagaga tagtgtcgat tagggccgat
    24301 cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa cagtcgacgg
    24361 caacatagcc gcttgaaaat catggagatg aaaaatcatc tgctgcgctg tgttcccagg
    24421 aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca tcagcaagta
    24481 ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc gcatctggtt
    24541 tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta gctgctggat
    24601 ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg gcagctgatc
    24661 caacatctct tttgtcatca tgacttcttc tggatactgt ccgttaggtt gcttgccgct
    24721 cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg cagaaagttc
    24781 ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat agctttgtat
    24841 tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga aatagttaat
    24901 acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta cttcatcgat
    24961 caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt gtattaaaaa
    25021 cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga aacgaacaaa
    25081 gtcttgttcg taaaatgact gatttcttgc taaaaatcca atacctctgt gcgcgatatc
    25141 tgcaaagaat gaatggttca aatttgttaa atgtaattca tccaaatcac cggtcttcgt
    25201 ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt gttctggcgg
    25261 ttccacaagt gtgcctttaa aacgctgtaa atcaatatag tggcgatagc ttttgataat
    25321 tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatgtctt tttctatgat
    25381 ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag gtgtagccaa
    25441 ataactcaat ggcagcagat aaccgattgt tttacccatt cctgtagctg cttcgatcat
    25501 caaatctttt gattcatctt ttgtgaaatg gtcgtagacc gcattcatca gtctgttctg
    25561 ttcttttcga tacatcagtg ttttaccaaa cattttttct tttgcttgtt tttttcttgg
    25621 atatgtccgc tcgccataat ggacggatgt gaatagttcc acttcttttt tctgcaaagc
    25681 tagcccatcg acgatttcta atgagggatc aagtggctct ggtttttctt tcatttcttc
    25741 aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta atcgcgcaat
    25801 ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca aaagaagttg
    25861 acccgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa atccaaggct
    25921 ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt gtgcaagttc
    25981 tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga gttcatggtt
    26041 caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg ccaaaagatt
    26101 gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt gattcgtgat
    26161 ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat cgatcgaaaa
    26221 acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga tccgatcttc
    26281 ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg gtttcaaaat
    26341 atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag tgcagaaaaa
    26401 tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa atgtctgggc
    26461 gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg aggtccgcgt
    26521 tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt gaatccgtcg
    26581 ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt gatgaatcca
    26641 aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa aatcctcctt
    26701 gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga tgtttatttg
    26761 aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca taccgtttac
    26821 aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc gattacataa
    26881 agtgaaaaac cgaacttttt ttaaaataaa cgacatattg ttcagttggt gtttttttgc
    26941 tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc ggttctgttt
    27001 caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg tatttctgat
    27061 agcctatgac agttaccaga agaacaaggc aaaccaatac gatcaacgag attagttttt
    27121 cttttttgct catatctata cctcataaat aacaggatga tgtccatcat atttctgagt
    27181 atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac tttcataatc
    27241 acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc
    27301 aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca gacgtgtgcc
    27361 tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta aacttaggga
    27421 tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat cgataagttt
    27481 ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc ataaatgatg
    27541 ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc tttttt
//
LOCUS       DAZKCN010000028        27260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000028 DAZKCN010000000
VERSION     DAZKCN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.028"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..547
                     /gene="nrdI"
                     /locus_tag="ACG8K3_001125"
     CDS             101..547
                     /gene="nrdI"
                     /locus_tag="ACG8K3_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9145018.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(612..2594)
                     /locus_tag="ACG8K3_001126"
     CDS             complement(612..2594)
                     /locus_tag="ACG8K3_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9145019.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2608..3504)
                     /gene="murQ"
                     /locus_tag="ACG8K3_001127"
     CDS             complement(2608..3504)
                     /gene="murQ"
                     /locus_tag="ACG8K3_001127"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9145020.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3507..4568)
                     /locus_tag="ACG8K3_001128"
     CDS             complement(3507..4568)
                     /locus_tag="ACG8K3_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9145021.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4779..5306)
                     /locus_tag="ACG8K3_001129"
     CDS             complement(4779..5306)
                     /locus_tag="ACG8K3_001129"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9145022.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(5373..6875)
                     /gene="eat(A)"
                     /locus_tag="ACG8K3_001130"
     CDS             complement(5373..6875)
                     /gene="eat(A)"
                     /locus_tag="ACG8K3_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9145023.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7133..8254)
                     /locus_tag="ACG8K3_001131"
     CDS             complement(7133..8254)
                     /locus_tag="ACG8K3_001131"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9145024.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8379..9320
                     /locus_tag="ACG8K3_001132"
     CDS             8379..9320
                     /locus_tag="ACG8K3_001132"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9145025.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9470..10366)
                     /locus_tag="ACG8K3_001133"
     CDS             complement(9470..10366)
                     /locus_tag="ACG8K3_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9145026.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10635..11975
                     /locus_tag="ACG8K3_001134"
     CDS             10635..11975
                     /locus_tag="ACG8K3_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9145027.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12193..12636
                     /locus_tag="ACG8K3_001135"
     CDS             12193..12636
                     /locus_tag="ACG8K3_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9145028.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(12901..14319)
                     /gene="nhaC"
                     /locus_tag="ACG8K3_001136"
     CDS             complement(12901..14319)
                     /gene="nhaC"
                     /locus_tag="ACG8K3_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9145029.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14722..15945)
                     /locus_tag="ACG8K3_001137"
     CDS             complement(14722..15945)
                     /locus_tag="ACG8K3_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9145030.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(15976..16815)
                     /locus_tag="ACG8K3_001138"
     CDS             complement(15976..16815)
                     /locus_tag="ACG8K3_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9145031.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(16832..17512)
                     /locus_tag="ACG8K3_001139"
     CDS             complement(16832..17512)
                     /locus_tag="ACG8K3_001139"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9145032.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17514..18587)
                     /locus_tag="ACG8K3_001140"
     CDS             complement(17514..18587)
                     /locus_tag="ACG8K3_001140"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9145033.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(18643..18881)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(18923..19156)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19233..19904)
                     /locus_tag="ACG8K3_001141"
     CDS             complement(19233..19904)
                     /locus_tag="ACG8K3_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9145034.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(19991..22639)
                     /gene="mgtA"
                     /locus_tag="ACG8K3_001142"
     CDS             complement(19991..22639)
                     /gene="mgtA"
                     /locus_tag="ACG8K3_001142"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9145035.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIHRFQELL"
     regulatory      complement(22981..23155)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            23328..23765
                     /gene="ndk"
                     /locus_tag="ACG8K3_001143"
     CDS             23328..23765
                     /gene="ndk"
                     /locus_tag="ACG8K3_001143"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9145036.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(23787..24653)
                     /locus_tag="ACG8K3_001144"
     CDS             complement(23787..24653)
                     /locus_tag="ACG8K3_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9145037.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(24813..26906)
                     /locus_tag="ACG8K3_001145"
     CDS             complement(24813..26906)
                     /locus_tag="ACG8K3_001145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9145038.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT         7791 a         5734 c         4802 g         8933 t
ORIGIN      
        1 tctcattata caagcaaagc gcctagtccc tttccaaatt acaccaccaa aaaacttaaa
       61 aaagatgcta tactaaaagg aaagaattgg aggatgatcc atgaacattt tatatatatc
      121 gatttcagga aatacgcgtg cctttgccaa acatctagca gaatatgctg aaaaaatgca
      181 tgcagaagat cctgtcaatc ctgaagttac tttaaaagaa atccatgaaa atagtgattt
      241 tacgaaagaa acagaaccat tttttacttt cgttcctact tatttagacg gcggaaatgg
      301 tttagataac ggtgatacag aaatcttgac cgaaacgatg cgtgaatatc tggaacatga
      361 agacaatcat aaactttgtc ttggtgtagt cggtagcgga aataaaaact tcaataatca
      421 gtattgtcta acagccaaac aatatgcaca aacatttggc tttcctttct tggcagatta
      481 tgaattacgg ggaacaccat ctgatgtgac acgaatttat cagattttag cagaaaacaa
      541 taaataataa aaaagaagaa gctgaaaaat agatttcagt ttcttctttc gtttctattg
      601 tttctatcat attaagcttt gacatccatc acaggtgctt ttgcttttgc tttacctgtt
      661 ttttccaaga caaagttttt tacacgatcc atattggtta ccaagacgat catggatgtt
      721 tctttgcctg ctgctttgat cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc
      781 acttgctgcc cttctgttac tttcacatca aatggcgtac cgcctaagtc aactgtgttg
      841 attcccatat gaagcaagat ttctaaacca tttggcgttg tgatcccaat agcgtgtttc
      901 gttgggaaga cagagctgac taccccagta actggagaat agatatgtcc gttttctggt
      961 tctacaaagt agccttcacc catcatcttt tgtgcaaaaa ctggatcact tgctttatct
     1021 aaagaaacaa ctgttccgtc agcaactgat gagaggatag tcacactttc agctgaagtt
     1081 tgaggtacta ctttttcagg cacttcattt actgtggctg tttcagtcgc taattcttct
     1141 ttttctaaac ggtctttagg aataccgaag aagaatgtag caacgaagcc accagcataa
     1201 gctgccaata atccaaggat gtagcctaac catttaccgt cagagatcaa tgggatcaaa
     1261 gcggctcctg aaggtccaat agcgatagcc ccgacatttc cgatcattcc gatcactgcc
     1321 ccgccgatac ctccaccgat acaagcagta ataaatgggc gtcctagagg taatgttact
     1381 ccgtaaatca atggttcgcc gattcctagg atcccgactg gtaaagctcc tttgatcatt
     1441 tctgttaatt ctttatcttt tttgcagcga atccataatg ccaatgctgc acccacttgt
     1501 cctgcaccag ccatcgcaag aattggaagc aatagcgtac gaccagtttc atcaatcatt
     1561 tgcacatgga ttggagtcaa aatttgatgc agtccgaaca taaccatcgg caagaataat
     1621 gtgcctagaa cgaagccggc aaatgctcca ccaacgccaa gtacccagtt gatcccaccg
     1681 accatgccat cagaaatgaa acctgctaaa ggcatgatga agaaaatttc aatcagaccg
     1741 atggcaatca atgaaagagt tggtgtaacg atgatatcta ccgcatcagg tactacttta
     1801 tgcagttttt tctcaacgat tgacaatagc caaacagcga agatgacacc taggatccca
     1861 ccttgtccag ctgcaagagc ttcaccagta aataagtttt tcagtggtgc ttccggattc
     1921 atccctgtca gcatcacaac ggccccgata actccaccaa gagttggtgt tgcaccaaaa
     1981 acttgtgcag cattgatacc tgtatagata actaggtagc taaacatccc gtttttcaag
     2041 atgttcatca catcgatata ttgttgccat gttgctgcat caagtgttcc ggctgtgact
     2101 aagtttgcta aaactgcggc aatccctgcc acgatcccag taccgacaaa tgcaggaatc
     2161 attggcacaa agatattaga aatgtctttt aataccgatt tgattttaga tggtttttgt
     2221 ttcgattttt gtgccgcttt catttcttgt gcccgttcgt ttacacggtc ttttccagat
     2281 gctgatgaat ttgctgtagc tgcacttaca gaagaaagtt tttctcctaa tttcacgcct
     2341 gccaaatcaa ccatttgctg tgctactttg ttcactttcc caggtccgat gatgacttga
     2401 agctgttcat cttctactac accaagcact cctggaatcg cttttaattc agagacattt
     2461 acaagatcat cattgatgat cttcatacgc acacgagtca tgcagtggac aatgctttct
     2521 acattgccca tgccgcctgc ccctttataa atttgttttg ccagttcttc caaagtcagt
     2581 tcgttttttg ccatggtctt ttccccctca taaggtccct tttacaaatc cgtttgcttc
     2641 agctaatttt gcttgagctt cctctttatc actatttgtc aaaatcatga cgatagccag
     2701 cttaacattt tgatctgctt cttcaaagta cttttcagct gtttcgtaat cagcttctgt
     2761 cgcttgcatg atgattcgtt ttgagcgttc gattagtttt tcatttgttg gtttgacatc
     2821 gaccatcaag ttattgaata ctttacctat accaatcata ctgatagtag aaatcatatt
     2881 caagataagt ttttgtgctg ttcctgactt caatcgagta gagcctgtca aaaattctgg
     2941 acctgcatcg acttcgattg ggaaatccgc gtgtttactg atttcggctt gtttattgca
     3001 agaaatcgtt cctgtgacag caccgatcga ttttgcgtaa tctaatccac cgatcacata
     3061 tggtgtacga ccgctagctg cgataccaat aactatatca ttttttgtca aattcagctt
     3121 gattagatct tcttgcccta gctcataaga atcttcggcc ccttcgacag ctacagtcat
     3181 tgcttcttct ccaccagcaa ttagacctat gaccatttcc ggttctacac cgaatgtcgg
     3241 tacgcattca gcagcatcta acacacctag tcttccactt gttccggctc ccatataaat
     3301 cagacgtcca ccttttttaa aggactcaat cgtgttagca atcactggtt cgatacttgt
     3361 cagttctttt tcaaccgcag cggccacttt ctgatcttct ttgttcatta gttgaacggc
     3421 ttgagaaata ctcatctcat ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag
     3481 gttctctaaa ttgattttgc tcaattttat cttccttctt ttcttataaa ttgatagttc
     3541 ataccagctt tgatttggtt gatcaatggt agctcttctt tgataacttt agctactaca
     3601 ttcacttttt catccgctgg caaatcatat ttggttaatt ggatctcacc catatagcgc
     3661 aaatactttt cgttatctaa agtaatagac cctttcatac gcgtagctgt atttcttgct
     3721 gggataggcg gaatctctcg aaaacgtgca tctgcgcttc gtatgacatc tcttgcatcg
     3781 tcttgccgat tcgtatgctt ccctaatata tattcgtaaa attcttcatc tacaggttcc
     3841 gtatggagca agactgtctg ttctttttgg aaagaagcaa attgctcctg aacgttttct
     3901 gacagacctg catctccgat gtaaacaata tccgtgttac ttgctaatag atctagagcg
     3961 cctgcaagag gatggatccc tcgatgttct tccaaagttg gcaatccttg atatagtggg
     4021 cctctcaact ctgtatctcc tggtacaaaa ccttggacaa ggagaccttg aattttgagc
     4081 cattgatttt ttttctgata ccattctttg tccagccctg tttcgggacg tggataataa
     4141 ttatgccaag cttcaatctg tgagaaatca gcttctgctt ctttcaattc atcgatatct
     4201 ttaggcgtga tcgtgctagc attcaaactg attttcaatt gatgtgacca gtcggcaatc
     4261 tgctgattgg aaatatgata atccattctt aaacctgtta caccaattgc tttcaaaggc
     4321 gttagatctt ctaatgaaaa acccgctcgt tccaatgcct ctcctgaaat atcgaccatc
     4381 aattctagtt tattcttttt cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc
     4441 gaaacatcat cttctggtat atgcatggaa gtgaaaatac cgacaaagcc attggatgcc
     4501 atttggcgga tatactgttt cttctcttct gacaaagaat cgttcataaa tattgaaaag
     4561 ccaaacatat tatccctctt tttctgtatc cgtcttcatt tctatgacta gagtatatat
     4621 ctaaagaaac aaaatttcaa tacttttatt aaaataatta aatttcattt ctaaaggttg
     4681 agtttatatt atcaaaagaa ttgtcataac ctaaaaaagg cactgctaag caaaagcagt
     4741 gccttgaaat attttattta aataacacaa aaacataatt actcataaga aaccaattct
     4801 gtattatcta ccatgaatgt aaagaacccc tcattaggca tcggatgaaa ataagcattg
     4861 atcactctta tcaaggcatt atggcaaact aacagataga cattttcttc gtcttcgatg
     4921 cattctttca ataaaggaac gatccgtgca tacacatcta agactgactc cccatttggg
     4981 aatcgtacag cgaacgctaa acgtgctttt tgaaagttct catctttgct tggcagtcca
     5041 tcataatccc caaaatccat ttctaccaat cgttcatcaa cggtcagcgg caagctgaga
     5101 cgatcagcaa ctgcttgtgc tgtatctctt gctcgttgaa gaggggaatg gatgattttc
     5161 gtgattggaa caggcaaatc tactaccttt tctgctagtt tctgtgcctg catctgtcct
     5221 ttttcagtca gcggcacatc tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt
     5281 tctccgtgtc tcgttacata aagcatgtga ttctctcctt cgttggcaaa gttcccctta
     5341 accatacctt gttgttcttg agattacaag ggttagattc tctcaagaga aataatctca
     5401 gtagatactt tatccaggaa ggtttgatca tgttccacta gtaacatggc aggtttcacg
     5461 ttcaagatca gttgttctaa ttgttcttga ttgaagacat ccaaataatt caatggttca
     5521 tcccatgtat atagttcagc tggctgtgat aaagatttag ccaattccac ttttttccgt
     5581 tggcccatac tcatctgctc gatcttgtta tgaaaaacat ctctttccat cccaagcttt
     5641 cggaggttgt tcaaaaatgc ttggtagtct acttggtttt tctctgcaaa ttccgccaac
     5701 gttcctcgat tgtcttcata attttggcgt acataactaa tgctcagatg ttttggctgg
     5761 gatttttctc ctataacctt gccgttgaat gcccccagaa gataatggat aatggatgac
     5821 tttcctgcac cgttaggacc tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga
     5881 attggctcga ataacagatt ttcataccct aattgaagat cttctacgct taaaagtcgc
     5941 ttatggtgag acgcttggct attcatcgtc aaagaatcga tatattcgat atcttttaat
     6001 agtttttctt tctcgctgat ctgggtctcc atccgattca cgattgcttt ggatcgtttc
     6061 atcgtccgtg cagcatcagc acccactgct cctttattca ctcgtctaga ttcagtatcg
     6121 atgaatccga cttgtttctt tgttttatct ccttctcggg aacgagacca ttcggctttt
     6181 tcagctgctg ttttctttag cctactgact tcttttttca atttttcatt ttgagccatt
     6241 tcaaattcat cacgaagttt tttctgttct tcatagatag agaaattccc ttgataaagt
     6301 tccagttgac ttttttcgat tgctaaaaca tggtccacta cttcatcgat aaatccccgg
     6361 tcatggctga ccacgatgaa gccttgtttt ttctttttca aataagccgc tacttgtttt
     6421 ctaccagaga tatccaaatg attcgttggt tcatcgatta acgggaaatg agtgtcatcc
     6481 acaaataaaa gtgccagtag gacttttgtc ttctctcccc ccgatagtgt gctgaattct
     6541 ctccataaga tttcaggatc tgtttgcatc aattggagct ctctttcgat ttcccatatc
     6601 tcaaaatccg taatatcatt taacacgtaa taggttaaac gttctttatc ttttgtctgt
     6661 tgcgggaaat aggcaaattc ttgctgatgg attacttgcc cttgataagg tagtttgttt
     6721 tgtagaatat tcagtaaagt tgtctttcct cgaccgtttc gtccgataag tcctagtttc
     6781 cattgtgtgt caaaatttag atttgcttgt tcaaataata atgtgccttg gctgtcgtag
     6841 ccgaatgtca gattttttat ttcgatttta gacattttcc atcacctcgt aaaaaaagac
     6901 cagtcctatt ttcagaactg gctaaagagg atgcaagggt tggatacaca caaaaagacc
     6961 catacatcga aaaaaccaag ccaatcccga aaatctgcac actgcttcct tgtctccaca
     7021 aggtacagtc tgaatgcagc tttcatcaat cgacttaatt tttcaatgca cggatccctc
     7081 actttgtcct atatttgaaa ttacgatagc ataaagaaaa tgattttgtc aactacttcg
     7141 ggaggatggt gaaaaatctg ctaagtgtct gtcctaagat ttttgttttt tctaatgttt
     7201 gcctgtgttc cattgcttct tctaaaccta ttggacccaa atgaatactt aagacaattg
     7261 ggagaaaatc agagagtcct tctagctctt ggtctctact tccgcaaata gcgattattg
     7321 gttttcgata tttttttgca agcactgcta caccgtgtgg aacttttccg tggatcgttt
     7381 gctgatcgat ccgcccttct cctgtaatga tgacatctgc ttgttcgatc tttttttcca
     7441 atccagtgat ttggctgact aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa
     7501 gggctccacc aataccgcct gctgctccac ttcctggaat atcatctaaa tccacctccc
     7561 agaaatcttt tatttgctga gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg
     7621 tatctattcc tcctttttgt cttccaaatt gccaagcagc tccttgttga cctgtatatg
     7681 gattggtaac atctgcagcg ataatcaatt ttactttaga aacaagaacg tttcttcgat
     7741 caaaatgaag ttttttagta gataataaag gatttccttc tgtgtaccct tctaaagaag
     7801 cacccaagga ttggagtaat cctagaccac catcgctaca cccgcttcct cctaaagtga
     7861 caataatttg ttctacttct tgaagcagtg catctttgat aagttctcct aaggcataac
     7921 tggaagcaag tcggatagtt tgatccgttg gtttatcaag tagatctaac cccaatgctt
     7981 gtgcagattc gatgactgcg gtattttttc cttgccaagt tgtcaataaa tagctgattt
     8041 ttcgctgacg gaataaaaga tccatcccat caatggtgcg ccatgttccc ccgagcgcgt
     8101 aatagattgc ttctgcagta ttttctccgc catctgcaat cggcacattg atttttcttc
     8161 ctttacgatc ttcccagcca tttagaactg cctgatttag ttcaaatgaa gaagcacttc
     8221 ctttaaatga atcaattgcc gccaaaatat ccatctgtat ccctcacttt cttcttaagt
     8281 aagacacttt ttttcatttt tttcaaacaa aacaatgcta ttttcttcca tttttctata
     8341 tactaaaagg gaactcattg ataaaggaga gaaaaaacat gaaaatcgca atagctggag
     8401 ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat gaggtcctct
     8461 tgatcgatcg ttggcctgca cacattgaag ccattcgtac aaatgggttg attgctgact
     8521 ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa atcatcgaaa
     8581 gcaacgaaca cgtggacttg atcgtagcat taacaaaagc gaatcaatta gatgatatgt
     8641 ttcgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta ctaaatggat
     8701 taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta tttgggatca
     8761 cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt gatggagaaa
     8821 tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt gtggaagtct
     8881 ttcaagaagc aaaattaaat ccgatctata gccataatgt tcgttattcc atttggcgaa
     8941 aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt aatattgctg
     9001 aactaggcgc gcaaaaaacg gcagagagca tggttcgaac aatcgtatct gaatttgctt
     9061 ctatcgcagc aaaagaagga atcatattag atcaagaaga agtgtaccaa catattgctt
     9121 ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa gacctgatca
     9181 aaaaccatcg tttgactgaa atcgactaca tcaatggtgc cgtttggcga aaaggacaaa
     9241 aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat gcaaaagaag
     9301 gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca gcagctggtt
     9361 ctcttttttt aagcccaatg aaagatcaaa aagaaaacac aactctcatg atgccataag
     9421 aattgtgctt gtttgtttcg gtacataatc tcttcaaaat gtaggttttt tatttatttt
     9481 caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta gtgataaatc
     9541 gttttgccca attttctgca tcataaaatt ccaatagctc tgatgggatt ggcttaaaag
     9601 gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa aaaatatttt
     9661 caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt ttcttttgaa
     9721 aatacataga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc aacagttcca
     9781 gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagcaaga tatccaggtt
     9841 gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt tttataaatt
     9901 cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca aaaaatagat
     9961 ccgtctttgg ttcgctagaa gaaaaatcca tatgtttcga ataaaatgaa ctattatgtt
    10021 tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg attgccggtt
    10081 catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc caaaaaaata
    10141 acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata ttaaatccat
    10201 aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat ttaatgaatt
    10261 ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat
    10321 aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt tccgcctcac
    10381 tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca gtaaaacgct
    10441 tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata aatcattctt
    10501 ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa tttgttatca
    10561 ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt gaatttaaaa
    10621 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
    10681 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
    10741 ttgggatcgc cggcccttct gtattgattg cttttttgat tggcgggctg attttatata
    10801 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
    10861 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
    10921 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
    10981 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
    11041 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
    11101 aaatcagtgt cattatctta ctaatcattt ttgctactta tcttgtcggt cagcaaatgt
    11161 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
    11221 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
    11281 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcca
    11341 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
    11401 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg
    11461 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgttattgtc cttgctttgt
    11521 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
    11581 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
    11641 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
    11701 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
    11761 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
    11821 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
    11881 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
    11941 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
    12001 tatgtggaat agatggaact attgaagaaa ggagttgtga caaaagtctt gttgcaactc
    12061 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
    12121 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
    12181 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
    12241 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
    12301 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
    12361 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
    12421 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
    12481 atgaaggcca tgattcctca tcattctatc gctatcctga caagtaaacg agctgattta
    12541 aaagatcctg aagtaaaaaa attagcggaa gaaatcatcc aggcacaaga agaagagatc
    12601 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
    12661 gaggatgttc cagaagtgat cagcttcaag aaataagaag aagctacttc tggaacaccg
    12721 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
    12781 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
    12841 cgtttattcg taaaagggat tgtgacaaaa aaagtcttgt cacaatccct ttttatgttt
    12901 ttattgttct aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttttttga
    12961 gattccccaa cctgtgaagg cactgaccat cgaaaacaca ggagaaagca aactgaaaaa
    13021 tacgaatggc aagtaagtga gtgtaggaat accaaatgta cttgctacga aacttccggc
    13081 aattccccaa ggcactaagt agttgattac tgttccacca tcttctagcg ttctacctaa
    13141 tacagttgga tcaaatcctg ctttttcata agtttcttta aatgcattac caggcagaat
    13201 aacagatagg aattgttcac caataaaaat atttactccg atacttgtca tcaatgttgt
    13261 aaaaatcaat tttggtgtgt tcgtcatctt tcttgatact cgttccatca cggttgagat
    13321 caagccaaat tcgatcaaca atccacctaa tgacaaagtt agaataatca atgacacagt
    13381 aggcatcata ctttcgattc ctccgcgact aagcagtgaa tcgatttgtt ggtttccagt
    13441 atttgaaaca aaccctgatt cgatgacccc agccaacttc gttaaggaag tttgtgggtt
    13501 ttgaataaaa atcattccta ctgctacagc gatattcaac aaaatcgtca aaattgccgg
    13561 cacctttttc catgcacaca gtaacattaa aaataaagga atcagtgacc agattgaaat
    13621 agtaaagttt gcttctagaa catcagtcac ttcacgaata gatgaaagac ttgcggcttt
    13681 acttgtgtgc cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg gaacagttga
    13741 ccacatcata tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctagatt
    13801 tgttgattcg gataatggag acatcttatc tccaaaaacg gcacctgaaa caatagctcc
    13861 aacaaccagc gctggattta ggcctagtgt tgtcccaata ccgaaaaatg caatcccaat
    13921 cgttgacata acagtaaagg cactccctac agcgctacct acgattgcac acacaacaaa
    13981 tacagaaggg acaaaccact ggacactgat caatttgaac ccaatgacca ttagagttgg
    14041 aatgatccct gcttgtatcc atacagcaat caatgaacct actaaaataa aaataaagat
    14101 tgggatgatt cctggtttga tgccattgat gattccttcg tgcagccaat ctgtcgggta
    14161 tctttttatc actgcataca acatgaccat tccgatcgct gctaagatcg tgatattagg
    14221 tgagaccttt aatccgacaa cgccaatcgc aatgcctaaa acaataacca ttaatacaag
    14281 tgttgcttct aaaaaacttg gtgttccttt ttttcccatg ataatctctc cttttttagt
    14341 tgttcttatg ttctaaaaaa gttaagatat cggattactc cgccgcaatt catccgctat
    14401 tcctccttta taaaaataaa aagacctgtt ttaaactaag gacgaatgac tccgcggtac
    14461 caccttaatt aaaaacagat ctgtttttca ctttcaaact tttattcaaa tgtatggact
    14521 ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca
    14581 aaacacctaa ccgctactgc gtgaaaagtt gacttgtatg aagtttacct tccataagat
    14641 catttgtcaa tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt
    14701 cacacacaag tttatttttt actagaaagt agcaccctct tgtttttttc ttgctactat
    14761 ttcttgatgt tcttgcttga ttctttctaa tagctttggc tctttcaaca gatcaagagc
    14821 tgtattggcg agtagttctg ctgcaatcgc aatagaagca aggcctttgt cacttttagc
    14881 agcagctttg aattcttctg tgtgtccggc aatatatgtc tcagagattg ctaccgtagg
    14941 ctggattgtt ggtatgactt ggctgacatt tcctacatca gaagaaccga gacttgttct
    15001 ttgttccgtt tctatttctt catctggaac acctgcttct tttgcatgct ggaagaataa
    15061 gtcatcaaaa gaaggagtaa cgatgacatc atcaactgca ttttggaaca atccaaactc
    15121 aaaagtcgtg cccgtactta tcgcagcacc tcgtacgata ttttccactt tttcatatac
    15181 tttatctaat gtttgccgat ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg
    15241 aatgacgttg gcagctactc ctccgtctgt aatgatccca tggatattga catcttttgg
    15301 caattgcaag cgtagagcgt tgattccatt aaacacttgg atcaatgctt ccaaagcatt
    15361 tactcctttt tcaggtgctg ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac
    15421 tggatcattc gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacgcataa
    15481 cgctacatcg acatcctcaa aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt
    15541 ctcgccccct tcttcccctg gggtgccata tacacggatt tctccaccag tttcatcaat
    15601 aaattgctcc actatgctgg cggctaatac ggagtaagta ccaaacaaat tatgcccgca
    15661 tgcatgacca atacctggta aagcatcgaa ttctgccaaa aaggcaattg tgggtccggg
    15721 tttattggaa atataacgtg catcaaaggc tgttcggtgt ccagctactt ctttttctac
    15781 ccaaaaccct tctttttcta attgtttaat caacgctgct gaggaataaa actcatagtt
    15841 acttacttca ggatgttcgt ggatatctag agcaagtgct tgatattctg gtaaacgata
    15901 ttggattaga tccttcaatt ggtttgttga gattgttgtt gtcataaaaa attcctccta
    15961 tgctagtcat ttttcttatt ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt
    16021 tgatgttttc tcgattactt tgactgtgtc ttctgtttga taagcgtcta ctattttttg
    16081 atacgtttta tttttttcat cttcttttct tgcaacgatg atattcacat aaggacgtgc
    16141 atggtcatcc actggttcta agaagatggc atcagaagca ggattaaatc cagcatctac
    16201 agctacaccg ctattgatca ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga
    16261 agcatctaat tcagaaatgt ctagattttt tctattctcg gtaatatcag aaacagtggg
    16321 cgcttgtcct tttgccttat ccactttgat caatccagca gtttgcaata acagaagagc
    16381 tctgccgcca tttgtaacat cattagggat tgctactttt gctccgtctt tgatatcagc
    16441 tacatccttc aaagtgttag aataaattcc cagcggtgca ttgacagtat ttccgatcga
    16501 tactaaatca gttccgtgtt ctttattgta attgtctaaa aagaattgat gttggaaaga
    16561 attcaaatca atcgatccat ctgctaaagc cgcatttggt tgattgtaat ctgtaaattt
    16621 cacgtattcc aaattaatgt tctccttttt caaacggtct ttcacgttgt cccatacatc
    16681 tgtatcttct ccaataattc ctagttttac ggtagtttct tttttgtcag ttgtatctgc
    16741 ttttccttgg ctacaccctg ccaaccaaat aattgctaca aatgtaatga ctacaaaacg
    16801 aataattttt ttcatttttt ctcctccact ttcaatgtgt aattttttta atcagttgat
    16861 tactgataaa ttgacttaaa aaaactaaga ttagaatcaa taatgtagcc acaatcgtta
    16921 catctgtttg gaatcgatta taccctctcg atatagcaag attccctaat ccgcctgcac
    16981 ctactgctcc agccatggta gtcagtccga tcacgttgat gatagtcaac gccgagaccc
    17041 gaatgatacc aggcaaacct tctaacaaat acacacgtaa aataataccg attggactgg
    17101 ttcccatcgc ttctgccgct tcgatcaccc caggatcaac ttctagcagt gcgttctcga
    17161 tttgacgagc aaaaaacgga acaactccaa aaatcaatgg cactaaagct gctttcgcac
    17221 caatcgtcgt tccaactaag aaacgagtag tcaatgctaa aagagaaagc aagataatga
    17281 aagggatgga acgaatgata ttgatcagct tatcaagtat ttcaaatagc agacggttag
    17341 ctagtaatcc attagggcgt gtcactacta acgtcacacc taagatgatt ccgatcatcc
    17401 cgccaatgaa tgctgtccaa aaagacatat atagtgtttg cttcgtcgct tcgacaaatt
    17461 catccggaat caaataaaca ttgggcaaat atgttttaaa gaaatcagtc attttatctt
    17521 cctccttcga tcactttgat acgactatct aattcgtttt tttgtaaaat atttattttt
    17581 acaccagatt tgatcaaata atccaatgct ttttgccgct gtttggtttc tccagctaaa
    17641 gtaacgatca aactccctaa cggcgtttct tgtaataact ctacattgcc atataaaata
    17701 ttcgctgtta cttgatattt gctgtacaga tgagcgatta gaggttcgtt cgtctgattt
    17761 ccaatataac ttaattccac cagccattta ttggcgatct gatcggcaaa agcagaatgg
    17821 gcaatgatcg tttctaatgc ttggtcaaga tgagtagctg ttcgaataaa atctttggtc
    17881 aattcttctt caggattgct gaagatctgg atgctactac ctttttcaat gattcttcca
    17941 tcttccataa ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc
    18001 aggacgattg ttaaacctaa ctggcgattc aattttttta acaatgcaag gatctgaagg
    18061 gtcgttttcg gatcaagtgc actggttgct tcatcacata ataatacctt aggttcattc
    18121 gccaaagctc gtgcgattgc tactcgttgt ttttgtcccc ccgacagttg tttaggataa
    18181 gcagatgctt tttcttccaa accgacaagt tccagtagtt cattcacttt ttgacgacgt
    18241 tcctgtttcg attttccaga atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg
    18301 ctttccatta gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat
    18361 tctttgggag agagctgact caaattcgtc tgattgacaa tgacttctcc tgctgttggt
    18421 ttctgcagca aattcatcac acgaactaac gtacttttcc ctgcacctga gtaaccgacg
    18481 attccataaa catcgccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct
    18541 ttttcttttt gtttgaaaca tacagaaaca tctcgcaatt caatcattaa aattcctcct
    18601 tatctaaaaa aagcgtcctc aagaaaatga aaatgtcatt ttcttgagga cgcttttttg
    18661 cgtgttacca cctcatgttc aagctgtgtt cacacacaac tcctcttcaa gtacgatcat
    18721 actctagcaa gatatcggtc gcagccgtca tcgccttaca tctatgctcg ggatgaattg
    18781 ctcgatggcc attttccaat agtcgttcct tgcttcttct cagctaccga agctctctgt
    18841 caaaggttct tctatcgtac ttaccatttc aatgcaaata atattcaaaa aaaaatcctt
    18901 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
    18961 agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc
    19021 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
    19081 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
    19141 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actaaaaagt
    19201 caagcacgaa attcaaatta ttttttctat atttattttt ctgttttttt atctaacatt
    19261 tcaataacag ttcgaagatc ttttccttca gcaagaagtt tcaatttttc cttttcttcg
    19321 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
    19381 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
    19441 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
    19501 tctccgaatg taaacgtgtc aaagtaatca gctaatttaa agtgatccaa gatatctgtt
    19561 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
    19621 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
    19681 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
    19741 actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt
    19801 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
    19861 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
    19921 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa ataacagaag caaagaacca
    19981 gccttctcgt ttataagagt tcttggaatc gatggatata gattttcttc acagctgtca
    20041 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
    20101 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
    20161 ctaatgtagt gataacaaac atggcaaatg ttacgttgct ttggataaat ggtactttcg
    20221 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
    20281 catggaataa tgcgatgaaa atcacttgct gtgaactatc caatgtatgg aaacttccgc
    20341 caactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
    20401 aacttgttgg gccaaagtaa accatgaatt tcccaatact agatgcattc catttttttg
    20461 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
    20521 agattagatt caaaaacaga agttgtaaag gcagcattgg taaaaatggc aggaacatac
    20581 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    20641 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    20701 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    20761 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    20821 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    20881 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    20941 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    21001 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    21061 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagtaaga taaccaatca
    21121 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    21181 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    21241 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    21301 tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag
    21361 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    21421 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    21481 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    21541 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    21601 ccgttttatc cgtgcataac acatcgattg caccaaagtt ttggatggca ttcaaatttt
    21661 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    21721 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    21781 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    21841 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    21901 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    21961 tgattccttc cgcacttcca cttatgacat tacttcccat aaagacaaga ttttcgtaac
    22021 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    22081 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    22141 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    22201 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    22261 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    22321 tgaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    22381 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    22441 tgtatgcttt caatatttct acgatgaatg gtgttttttt gccatatgaa atcgcatttt
    22501 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcgt
    22561 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    22621 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    22681 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    22741 agatagataa tagccttggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    22801 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    22861 agttattttc ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    22921 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    22981 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    23041 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    23101 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    23161 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    23221 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    23281 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    23341 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc attcaacgtt
    23401 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    23461 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    23521 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    23581 gtaagatggt tggggcaaca aaagctgtcg atgctgttcc aggtacgatc cgaggagact
    23641 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    23701 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    23761 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    23821 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    23881 tggtagtctt tcgtcttttt ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    23941 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    24001 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    24061 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    24121 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    24181 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    24241 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    24301 accatgtgaa gactcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    24361 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    24421 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    24481 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    24541 gaatttgtct caatcccgac tagagaagtc ttagaacaga ttccttccgc taattgggac
    24601 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt
    24661 ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata
    24721 cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat
    24781 gttgtctaac tactgaaggt tggtttgatt gtttattgat tatctttttg ctgttcaacg
    24841 tctgttcttg ttactggata taagttggtt gttgttttat tgccattttt tgaaaagaca
    24901 cttgtcgaat gttcgccttt ttgcttttcg atggctatgt tacgttctgc ttcattttta
    24961 tagtcatatt ttttcggatc gatcgtttca aatccgtttg gcacataaaa acgcaagaga
    25021 ttttcttgat tcagcttatc tgaaagactt aaagcagatt tgactttttc ctgatctttg
    25081 gaaatttcct cgtcgattcc tgctgctttc gtatcgatga tttctcctgt ttggttattg
    25141 taagcttttc ctcctaagat ggtgtatttg ggtgtcacga agttcccatt tcgaaaagcc
    25201 actacttgat catgctgagg agataataaa tccgtaccaa aatggagata gtctttatca
    25261 tcgattccca agagatgaag taatgtcggc aaaacatcga tttctccacc gtattcgtga
    25321 ttgactgacc ctttagtata accaggtata tgaatcatta atggcacacg ctgcatttgt
    25381 gcgttgtcaa attcattcca tgtatctggg tcttttccaa gagcacttgc taagtcttta
    25441 ttatctgtat ttgagatgcc aaaatgatcc ccataaatca caaagatcga attttggtaa
    25501 atagcggatt ttttcaggta cgtaaaaaat tgttccaatg actgatccaa ataatgtgcc
    25561 gttcgaacat agttatctac tgtgctattt ccagtgttca aagatggaaa atcgaaattc
    25621 ttatcatccg tataataagg cgtgtgattg gttacggaaa ggaatttcgt gtagaacggc
    25681 tgttgtagat gttcaagata ttgggcagat tcactgaata aatctttatc taatattcca
    25741 taacctagcg tttcatctga ttcatcaaaa taagatcgat caaagaaatt gtcataacct
    25801 aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg gaaaacagcg
    25861 cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac attatctgac
    25921 ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatattt
    25981 tcagcatcac ttgtctttcc ctgtcctact tcatgaaaaa aattatcaaa gctgatcgtt
    26041 gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc taccttcata
    26101 ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt ccctttagcg
    26161 atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc taaaacttca
    26221 tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattcca
    26281 tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg gtcaaatgtt
    26341 cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga aaaaataagc
    26401 cctgaaagac aggtcacagc gatagccatc ggtttgctga caggttgact tttgataggt
    26461 attttcttga tcgtcaacca aaccagtatc ccaatgaacg tagcaatatc cagccagtag
    26521 aggacatcat ggggtttcat caaagaaaag gagcttcctg ataacccttg cgacactttc
    26581 gaaaatccca atacagattt gactgtaatg aaatctgtga attcccgata aaaaataatg
    26641 ttcaagtaca acaaaagcgt gtttaaaata tcaatgatca aaagaatggc taatgctggc
    26701 tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagttgc aatcgggttg
    26761 atccacaaga taaagtactg gatcgtacct tccacaccta acgaaaaatc tgcatagtaa
    26821 gcaataatcg tcttcatcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt
    26881 tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca tttttcctta
    26941 ttttgctcct gaaaaatgaa aattccctaa aaaagttgtt atcttaagat ttttttattc
    27001 tttctaaaga ttattccgtg cacttttcac agcataaaac cgctgtgata taagcgattg
    27061 aaacagtaag gtgataaaag tcttgtcgca gctcctgttt agttgatgca ggacagcttt
    27121 ttcacaactt cttctttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    27181 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    27241 aagctcaaga cctcttgata
//
LOCUS       DAZKCN010000029        26964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000029 DAZKCN010000000
VERSION     DAZKCN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.029"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..2290)
                     /locus_tag="ACG8K3_001146"
     CDS             complement(137..2290)
                     /locus_tag="ACG8K3_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP9145039.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTNNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="ACG8K3_001147"
     CDS             complement(2583..3431)
                     /locus_tag="ACG8K3_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9145040.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3653..5596)
                     /gene="thrS"
                     /locus_tag="ACG8K3_001148"
     CDS             complement(3653..5596)
                     /gene="thrS"
                     /locus_tag="ACG8K3_001148"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9145041.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSRENK"
     gene            complement(5933..6112)
                     /locus_tag="ACG8K3_001149"
     CDS             complement(5933..6112)
                     /locus_tag="ACG8K3_001149"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP9145042.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6159..7982)
                     /locus_tag="ACG8K3_001150"
     CDS             complement(6159..7982)
                     /locus_tag="ACG8K3_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9145043.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHAHSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8260..10539
                     /gene="helD"
                     /locus_tag="ACG8K3_001151"
     CDS             8260..10539
                     /gene="helD"
                     /locus_tag="ACG8K3_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP9145044.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10632..11279)
                     /locus_tag="ACG8K3_001152"
     CDS             complement(10632..11279)
                     /locus_tag="ACG8K3_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145045.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11458..12177)
                     /locus_tag="ACG8K3_001153"
     CDS             complement(11458..12177)
                     /locus_tag="ACG8K3_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9145046.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12308..13459)
                     /locus_tag="ACG8K3_001154"
     CDS             complement(12308..13459)
                     /locus_tag="ACG8K3_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP9145047.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13676..14416)
                     /locus_tag="ACG8K3_001155"
     CDS             complement(13676..14416)
                     /locus_tag="ACG8K3_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9145048.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14420..14776)
                     /gene="rsfS"
                     /locus_tag="ACG8K3_001156"
     CDS             complement(14420..14776)
                     /gene="rsfS"
                     /locus_tag="ACG8K3_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9145049.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(14800..15393)
                     /gene="yqeK"
                     /locus_tag="ACG8K3_001157"
     CDS             complement(14800..15393)
                     /gene="yqeK"
                     /locus_tag="ACG8K3_001157"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP9145050.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15377..16027)
                     /locus_tag="ACG8K3_001158"
     CDS             complement(15377..16027)
                     /locus_tag="ACG8K3_001158"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9145051.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16037..16348)
                     /gene="yhbY"
                     /locus_tag="ACG8K3_001159"
     CDS             complement(16037..16348)
                     /gene="yhbY"
                     /locus_tag="ACG8K3_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9145052.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16364..17473)
                     /gene="yqeH"
                     /locus_tag="ACG8K3_001160"
     CDS             complement(16364..17473)
                     /gene="yqeH"
                     /locus_tag="ACG8K3_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP9145053.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17470..17997)
                     /locus_tag="ACG8K3_001161"
     CDS             complement(17470..17997)
                     /locus_tag="ACG8K3_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP9145054.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18224..19009)
                     /locus_tag="ACG8K3_001162"
     CDS             complement(18224..19009)
                     /locus_tag="ACG8K3_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9145055.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(19002..19871)
                     /gene="accD"
                     /locus_tag="ACG8K3_001163"
     CDS             complement(19002..19871)
                     /gene="accD"
                     /locus_tag="ACG8K3_001163"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9145056.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19872..21251)
                     /locus_tag="ACG8K3_001164"
     CDS             complement(19872..21251)
                     /locus_tag="ACG8K3_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9145057.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFFSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21259..21678)
                     /gene="fabZ"
                     /locus_tag="ACG8K3_001165"
     CDS             complement(21259..21678)
                     /gene="fabZ"
                     /locus_tag="ACG8K3_001165"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9145058.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21703..22179)
                     /gene="accB"
                     /locus_tag="ACG8K3_001166"
     CDS             complement(21703..22179)
                     /gene="accB"
                     /locus_tag="ACG8K3_001166"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9145059.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22182..23417)
                     /gene="fabF"
                     /locus_tag="ACG8K3_001167"
     CDS             complement(22182..23417)
                     /gene="fabF"
                     /locus_tag="ACG8K3_001167"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9145060.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23436..24173)
                     /gene="fabG"
                     /locus_tag="ACG8K3_001168"
     CDS             complement(23436..24173)
                     /gene="fabG"
                     /locus_tag="ACG8K3_001168"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP9145061.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24185..25102)
                     /gene="fabD"
                     /locus_tag="ACG8K3_001169"
     CDS             complement(24185..25102)
                     /gene="fabD"
                     /locus_tag="ACG8K3_001169"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9145062.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25209..25433)
                     /locus_tag="ACG8K3_001170"
     CDS             complement(25209..25433)
                     /locus_tag="ACG8K3_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9145063.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25486..26448)
                     /locus_tag="ACG8K3_001171"
     CDS             complement(25486..26448)
                     /locus_tag="ACG8K3_001171"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9145064.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26458..26898)
                     /locus_tag="ACG8K3_001172"
     CDS             complement(26458..26898)
                     /locus_tag="ACG8K3_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9145065.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7362 a         5975 c         4693 g         8934 t
ORIGIN      
        1 ccttttttgc cgagtaaacg gtgttcaaaa gccagtttct cggtatgaag tcaaatttaa
       61 gcaaaaatgt gagaagctat tttcacaaaa tttttcttca tactcgaaac taagcggctt
      121 tttcacgacc tcttttttat tttgccatga acatatcata atacgcgtcc attatttctt
      181 tagcaatcgt caagttcatg tggtcttgtt cgtctaataa gttcggcaag acaacactga
      241 tggcaatttc cggattgttt gttggtccgt atgccacgat attactattg actgtcgtaa
      301 tatctggatg accttccgca accgtttctg cagtccctgt ttttgcggaa aggtccattt
      361 tcgcactggc taatggtttg gctgttgtgt aaggatcggt accatggaca acttggtgga
      421 atccttcacg gaggatcgcc atattttctg cagaaatatt gaccgtattt tctactgttg
      481 tgccaatttc tttcttcact tcacccaagt ttccattttc gtcattgcca tagatcgctt
      541 tgactaggtg cggctgtact cgtttcccgc cattagcgac agttgaagca tattgagcta
      601 attggattgg tgtatatgtg tcaaactgtc caaatgccaa atcggtaaag ttacctggag
      661 tataccattc tttgccatta tccgcattga acttcttcat ataatcaaca gaacgtgaga
      721 tcccaggaga ttcattcggc aaatcgatcc cagtcgttgt accaagacca aactctttga
      781 aagagtctct caattgctga taagctttag cttcttcatc taaactaggt agtcccattc
      841 caggtgtata gtctagtcct aacatcttta acgccacttt gatcatatac gtattagaag
      901 acaattccaa tgctttgact gcatcaagag caacttgtcc gcttcgatta aagacagagc
      961 ttttttctga agcaccttgt agacgaatcg gctcatcaat caaaacttga tttccgctga
     1021 tcgcgttact tgcccagcct gctgtcaaag taccagcctt gactacagac cctggagcaa
     1081 aggcgctagt aatcgtgcct aaagcattct cggtgatttc tttggaacct tgttcatgac
     1141 tgaaaccagt cattgctagc acttctcctg tctgaggatt catagccact gcataagcac
     1201 caggcgaata ctgtgctttg ccgttgttga tcaatgtttg ataattcctt ttcaaaattt
     1261 cttctacttt tgattggaat tctgcattca tagataacat taaattagaa cctttttccc
     1321 cagagaagat ttctttttga ttcgaaacat ttccttcatt atcaagggaa acttcatatt
     1381 gggttttggt tccttgcagg acatcttcat attgtttttc caaataactt tgtcctacac
     1441 ggtcattccg agaataacct tttgctaggt atttctctgc ttcatctgct ggaagtcctt
     1501 gcttttcagt cgtcacactg ccaagaatac ttttcagtga acttgctgca ttatattctc
     1561 gtgtccagtc tgtgccagtc gaaactcctg gaagttcaga agcgcgttct gccacaacag
     1621 ctaactcttg atcagtaaca ccagagtttt tgatatagac agtgtttaat tcatatgcac
     1681 tattcatccg tttaaagatg gtagcaattt ttaattgctc ctcgtttaaa ttaatctcat
     1741 cagctgtgac tttattgacc atcgcactat attgttcgct gttccctaat tgcttttctt
     1801 ttgccgaaag tctttttgtg gcttctttca aatgtttggg atcagccagc caaaaatctt
     1861 ttttatctct atctgtcaga ttttcatcta ccggaacatc gatcagcgag ttcagcttcg
     1921 ttgcgatttc aagcaaatct ttagccgtaa cttgtgttcc tctcgtatat gtgatggcct
     1981 gattcgcttt attcgtaact aaagcttttc ctgtggagtc gtagatcatt ccgcgaggag
     2041 tactttcttg gaccgtaatc gtagaagtag acttcactcg cgcctccatc tcttcgccgt
     2101 tcacgatctg caaataaccc aatctagcaa tcaacgcaac aaataacgta aatataatga
     2161 aaaaaagaaa atttaagcga aaaggaacat gggaccggcg aaacgttttg ttcttaggtt
     2221 cttgtgcacc ttttcttatt ttttctaacc aagaagtatt taatattttt ttcataaaat
     2281 ctttcatcat ttcctatcct tccgcctatt tacattcatg cttcattgta cccaaaaact
     2341 tcttaataaa aaagaaaaaa tgggcggagc aagaaattct ttacctaatt tcaagataaa
     2401 atcaactttt tgtttcacag caatcactta aagttacata tccaactatt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttgtta acggtcgtgt actggataaa aaaacaaaaa aacaatccgc acaataggat
     3541 atcttactcc atcaatccca caaattctat tgtccatctg tgggattgtg gaatatctgt
     3601 tctctattgt ttggattgtt ttttatttag tgctttttta taggcagctt atctatttgt
     3661 tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata ttcaaatctt
     3721 ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct tccaattcct
     3781 tgtctccaac aaccaattgg taagggatct tttgtgtttg agaggcacgt atcttatagc
     3841 ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc aaacgttctt
     3901 tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt gttgcttgta
     3961 ttggggccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa gcaacaaaac
     4021 gttccattgt agaaacgatt ccgcggtgga taactactgg acgatgcgta ttttccccgt
     4081 cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat tgtatagttg
     4141 ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt ggtccgtaaa
     4201 aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata gcggctttca
     4261 gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct gtattgtttg
     4321 gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca tatactgcca
     4381 ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga acaaatgtat
     4441 gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca cctgattttt
     4501 catagcgatg catcattcct aattcagcga tacgaattgg taattcacga taagaatgga
     4561 tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tggacgaaga acaagcattt
     4621 cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag
     4681 atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg taacctagac
     4741 taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa
     4801 gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca agttcttttc
     4861 ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat tctttcaaag
     4921 ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg tttgagtttc
     4981 cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg ccagtggatg
     5041 gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca gtaatcactt
     5101 cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca aaaatttcta
     5161 atgcttcctg ttttgaaaga acacggcgtt cgattggcag attttctttg acgattttca
     5221 tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt ccattatctg
     5281 tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata tttggataga
     5341 ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag ccaagtgcat
     5401 cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga cgagtcaaat
     5461 caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt tttgaaatgc
     5521 tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca ccatctggaa
     5581 aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca gatcatcaag
     5641 ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga aaagttcgtg
     5701 gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc
     5761 ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga aaagtttcag
     5821 cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc atcaatcaat
     5881 tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa acttattttt
     5941 tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg acatggatgt
     6001 gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc atcatatttt
     6061 ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc ataatgctct
     6121 ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt tgtcttgggt
     6181 actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa aatagagacc
     6241 gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc cgtacttgca
     6301 attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt agctattctc
     6361 atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc tttcaagatg
     6421 accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc aataccgata
     6481 tctgcagcag caagtgctgg agcgtcattc acgccgtctc ctaccatcgc aacaggtctt
     6541 tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct atgggcatgt
     6601 acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc atccccggtg
     6661 atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt tgctgcttct
     6721 gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat tgttacatag
     6781 attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg tccaatcttt
     6841 acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt cagttgacta
     6901 acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa gatatggcta
     6961 gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat tccattgact
     7021 ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt atcaaaagca
     7081 atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac gatcccgttt
     7141 ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat cagtggacaa
     7201 ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa
     7261 ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc agctaaacgg
     7321 acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat ttgatactgg
     7381 ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt taaagaccct
     7441 gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt caaagaagat
     7501 tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag ttcgccaggc
     7561 ttgactagta actgatcatc aatatttatt tcatcgatta ctatatcctg caattttcct
     7621 tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag ttctttaccc
     7681 gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat caatatcatc
     7741 aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat cgctaataca
     7801 tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg
     7861 agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat
     7921 tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt gaaatgtgtc
     7981 attgtttttt ctcctctttt atgtaactta gagaggttag taataaattt ccagctgcct
     8041 ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc ttaattgaaa
     8101 acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa atattcagca
     8161 aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac tatgctatac
     8221 ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa acaactagaa
     8281 caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat gctggataca
     8341 aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc taatcacaag
     8401 attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg ccaacatgag
     8461 caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct gcaaacgttg
     8521 tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca
     8581 gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac aatcgttatt
     8641 gactggcgtg caccaattgc caatctttat tatgaaggtg agattggtcc cgcattttat
     8701 gaaactgaaa tagatagaat agatgtcgat ctgcagttga aacgacaatt taaaatccaa
     8761 gaaggtcgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga ctttttgcta
     8821 gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa
     8881 gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga aggaatcgcc
     8941 ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta tcataatcgc
     9001 aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt ttctgattac
     9061 atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac attcaaaaat
     9121 tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca agaaagtggt
     9181 tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac cttagtcgat
     9241 cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg tgatatgaaa
     9301 ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa agaaacaaat
     9361 gaacaattgc ctttacatca tagactctct ctattacaaa cgaagctcct gaaaaaatta
     9421 ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc ggaagaacag
     9481 ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa ggagcgttca
     9541 ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg
     9601 atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct acaaactgtg
     9661 ccactttctc ttctaacacg acataaaatc agcgagacca gatggcagga acatttgaca
     9721 actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt actattcttt
     9781 ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta tatctttatt
     9841 gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt gttcccaaaa
     9901 gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga aacgatcgtt
     9961 ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt aacgacaagc
    10021 tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa agaacatcaa
    10081 gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac aacagatgat
    10141 atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc ccggtattgg
    10201 cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc tcaactgcca
    10261 gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat gaaacgttcc
    10321 attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg
    10381 gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta caccatgtgt
    10441 acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc tttgttaaac
    10501 aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca actgctatca
    10561 ttaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt aattcaatca
    10621 tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt taatttgaca
    10681 agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc aaataccttg
    10741 ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat
    10801 tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat ctctgataca
    10861 cgaaatgcta ggtatgcact atcgatcaca ttattttttt tcgtgtctat cttctcattt
    10921 ctttcgataa gaattttttc aggacgatgc ttcaagctcc actctaatag tagaaagaaa
    10981 gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc
    11041 agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac catacactct
    11101 aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc ttcaacgccg
    11161 aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc taactcttct
    11221 actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt tgcttccata
    11281 ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt atgctctcat
    11341 cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt
    11401 agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac tggttgctta
    11461 gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt ccagtgcatc
    11521 aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt tttgagggat
    11581 catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg cttcttgtat
    11641 actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt ccattacatc
    11701 gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc
    11761 aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt
    11821 cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca cattgttcgt
    11881 caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc gcaattcaga
    11941 gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa tatgttttgg
    12001 tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg catgcggatc
    12061 aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat aaatccggct
    12121 attattagca tctttagcag ttttctttgc tacgatattt gcccatttac gtcccatatt
    12181 ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt ccattatact
    12241 tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct catttttagt
    12301 caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata ttgcctccca
    12361 attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct ttttttccga
    12421 ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca acatcggtaa
    12481 atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc tttttgatat
    12541 tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt tttgttttta
    12601 cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc atgcgcgatt
    12661 ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa gataacaacc
    12721 gataacgcaa atacggccaa taagattccc aagtctgcag cgaataagtt gaaagatctt
    12781 cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac gcatggcgaa
    12841 tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag cctgcacttt
    12901 tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc tttgcataac
    12961 ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga tacagactgc
    13021 gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt tcttgaaatg
    13081 cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg taatcaaacg
    13141 aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc aagattttta
    13201 ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga agctccacta
    13261 ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg atgatagccg
    13321 gttcgcctcg ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct gctcctgttg
    13381 tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg tactctacga
    13441 taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa agaaatagag
    13501 acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact attctcgcaa
    13561 acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat cgttcggata
    13621 tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa aaccgctagt
    13681 ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg tctgtaaaat
    13741 cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa taactttcca
    13801 atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca gacccttctt
    13861 gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt cctggataac
    13921 tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct ggaaaaattt
    13981 catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct tctaacgctt
    14041 gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag cacagcgaat
    14101 cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc atattgcctt
    14161 ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc atcaacatct
    14221 cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca actgctaatg
    14281 ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg
    14341 aaaaagctag ccattgctga taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa
    14401 atgtttcata agccatatgt taatccaacc attcactaag atcgactagc ggagcatctg
    14461 accatagttt ttccagatta tagaaacttc gttctggtgc atggaaaaca tgtacgatca
    14521 catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata cgttttactt
    14581 catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg atttgacgtt
    14641 cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt cgaatgtcta
    14701 aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aaccgcaatt tttaattgtt
    14761 ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg ctacccagta
    14821 attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca aatgggccaa
    14881 tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa tggctcgtgc
    14941 atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat aaatgatttt
    15001 gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat gaagaatttc
    15061 tacatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac catgccagat
    15121 tgcattatta taattcaata actctggatc aaaaccgtct cttacgatta ccattttgaa
    15181 ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac tagctttttc
    15241 aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc caagcacatg
    15301 cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt ctctagaata
    15361 agcagtatat tcattactat agtccatatt catacaaccc ttcattttga atataatcaa
    15421 ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt tgacgtattt
    15481 tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat ggagtatccg
    15541 ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc tcatcgattt
    15601 tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa taatctgtat
    15661 ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc ccaccacgag
    15721 ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc cacagctaat tccaacatgt
    15781 tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc tgataagaag
    15841 gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc atcactaaat
    15901 gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt ttcttctcta
    15961 agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat
    16021 tcatcatggt cactccttat attgctttta cttcttttga tagcctttga tacttttctt
    16081 ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt tcgcaatcta
    16141 tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt tgaagtaacg
    16201 atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc attgctgaat
    16261 tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa
    16321 aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc aatgctttgc
    16381 gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc gctgttacag
    16441 tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa aactcaaaac
    16501 gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat aatccaccta
    16561 catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata tttacatcat
    16621 ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc
    16681 cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg atttctttta
    16741 acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga tggcgatgga
    16801 taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg attttatcca
    16861 aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct gtatttttga
    16921 tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata tagacatccc
    16981 gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga gatttcttag
    17041 cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt tccttcatcc
    17101 attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg ttcccaacta
    17161 aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa ccattgaagt
    17221 caaaaatatc caccacattg acgatcaatg catctttctg ccctaattca ttcagtaagc
    17281 gcaaaaagtc gtcatccgtc aatgagacat cttggatctc attataatgg cgtaatctga
    17341 aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg
    17401 tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg atacattgta
    17461 attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg ctaatagata
    17521 ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat
    17581 aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta tatcagtcat
    17641 aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt tctttgctat
    17701 gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat caaatttttc
    17761 aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag ggattcctgc
    17821 atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat tattccatgc
    17881 aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct ttaattgggc
    17941 aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg aaaacatgct
    18001 gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc cgtattatac
    18061 tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa agcatttctt
    18121 tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa aaacaactaa
    18181 gcgagcaaga ccattcttta ctctttgctt agttgttaaa cacctaatat tggcggaacc
    18241 tcttgtatcg tttttccagt aattcatgct ggtcgaatcg aacaagttcc gtcaatcttt
    18301 caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga
    18361 acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt
    18421 tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc
    18481 cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac
    18541 ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct
    18601 ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg
    18661 ctcctacacc gcaatatgca cctgctgtat tgatcaatgt aatcaccggt ctattgaact
    18721 tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa
    18781 aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg
    18841 gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca aacaaacgat
    18901 caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc
    18961 gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat
    19021 cctttctgtg tatgcaattc aagaagcgta tggattttct gcttcaattc catgcgggga
    19081 acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga
    19141 agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg
    19201 ggttctgcta gaatgatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc
    19261 gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca
    19321 gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa
    19381 gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc
    19441 ttttctccga caatcgttcc catacttccc atgataaaat taggatccat cactccaata
    19501 atgaagggaa tcccttgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt
    19561 ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt
    19621 acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc
    19681 catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct
    19741 ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat
    19801 ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta
    19861 aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct
    19921 tcttgtaaaa atgctgtgct gtaatcacct gctatgactg cagggtgact aatcaaatcc
    19981 atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc
    20041 atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa
    20101 tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctacccg cagtcccatc
    20161 ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca
    20221 ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga
    20281 cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct
    20341 gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa
    20401 aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct
    20461 ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca
    20521 gaaggagatt cttccagtac tttttgatta ttacgttgca aagaacaatc tcgttctcct
    20581 aaatgaatga cattgccaaa atgatcacct aaaatctgaa cttctatatg gcgagcagga
    20641 taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga
    20701 gctgaaaaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg
    20761 ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat
    20821 gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct
    20881 tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga
    20941 ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa
    21001 aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc
    21061 tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct
    21121 aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct
    21181 agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact
    21241 ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt
    21301 accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc
    21361 aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa
    21421 tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc
    21481 tgcttgagcc atcgcttcaa caattaatac ccctggcata acaggttctt ctggaaaatg
    21541 tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc
    21601 aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt
    21661 tatttcttgt atgttcattg atttccctct ttcctgcttt gcttattttt gctgaatcct
    21721 aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac
    21781 tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac
    21841 ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgccggttg
    21901 cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc
    21961 tttttctttc attgtttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc
    22021 cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt
    22081 attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc
    22141 aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc
    22201 atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa
    22261 cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct
    22321 acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg
    22381 atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta
    22441 tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct
    22501 ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct
    22561 tccatcgcaa gcttcattgc tttagacgca ccagaaccat ctggagtagg agctgtcata
    22621 tggtaagcgt cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga
    22681 gcttgtgcat gttctaaaga ttcaagaact agaacacctg ctccttcacc catcacaaaa
    22741 ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt
    22801 gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca
    22861 gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc
    22921 gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc tacgcgcaaa
    22981 gcaatatttc cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt
    23041 tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc
    23101 ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc
    23161 atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg
    23221 ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta
    23281 atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt
    23341 aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta
    23401 attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca
    23461 tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt
    23521 gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg
    23581 actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta
    23641 catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca
    23701 gctttactag ctgcataatt tgcctgacca atatttccaa tcaacccaga tacactagag
    23761 agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata
    23821 ttgaaggttc caatcaaatt gatatcaaga cattttttga aatcttctgc atccattcgc
    23881 atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc
    23941 ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa
    24001 actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct
    24061 ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg
    24121 ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc
    24181 ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg
    24241 gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga
    24301 tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt
    24361 gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg
    24421 tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca
    24481 gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga
    24541 tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt
    24601 cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact
    24661 tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca
    24721 ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg
    24781 caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac
    24841 tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga taatacgcga
    24901 tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg
    24961 taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct
    25021 ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac
    25081 taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct
    25141 tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat
    25201 tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt
    25261 ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat
    25321 aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca
    25381 gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata
    25441 tattcctccg atattgtttg ctttttattt ttataattta acagtctaca actccatgag
    25501 aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc
    25561 caaggtgatc tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga
    25621 tgtattccca tatttatcca tattggtcag aaacttttct tgcggcatct ttacttttcg
    25681 ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt
    25741 gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc
    25801 tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt
    25861 attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc
    25921 tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa
    25981 caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat
    26041 cactagtccc ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac
    26101 aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc
    26161 ttgtacttgt gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat
    26221 aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat
    26281 gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga
    26341 cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt
    26401 ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca
    26461 cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc
    26521 ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac
    26581 ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc
    26641 actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt
    26701 cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc
    26761 gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag
    26821 ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt
    26881 ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga
    26941 tgttcaaact atatcttaac tttt
//
LOCUS       DAZKCN010000030        26631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000030 DAZKCN010000000
VERSION     DAZKCN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.030"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACG8K3_001173"
     CDS             complement(19..729)
                     /locus_tag="ACG8K3_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP9145066.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACG8K3_001174"
     CDS             complement(726..2198)
                     /locus_tag="ACG8K3_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9145067.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACG8K3_001175"
     CDS             2336..2809
                     /locus_tag="ACG8K3_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9145068.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACG8K3_001176"
     CDS             complement(2962..3513)
                     /locus_tag="ACG8K3_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9145069.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACG8K3_001177"
     CDS             3618..4148
                     /locus_tag="ACG8K3_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP9145070.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACG8K3_001178"
     CDS             complement(4631..5074)
                     /locus_tag="ACG8K3_001178"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9145071.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACG8K3_001179"
     CDS             complement(5090..6250)
                     /locus_tag="ACG8K3_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9145072.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACG8K3_001180"
     CDS             6757..8457
                     /locus_tag="ACG8K3_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145073.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACG8K3_001181"
     CDS             complement(8559..9392)
                     /locus_tag="ACG8K3_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9145074.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACG8K3_001182"
     CDS             complement(9696..11861)
                     /locus_tag="ACG8K3_001182"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP9145075.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACG8K3_001183"
     CDS             12121..14415
                     /locus_tag="ACG8K3_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9145076.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG8K3_001184"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG8K3_001184"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9145077.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACG8K3_001185"
     CDS             15066..16073
                     /locus_tag="ACG8K3_001185"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP9145078.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACG8K3_001186"
     CDS             16095..17114
                     /locus_tag="ACG8K3_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9145079.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG8K3_001187"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG8K3_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP9145080.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG8K3_001188"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG8K3_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP9145081.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG8K3_001189"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG8K3_001189"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9145082.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG8K3_001190"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG8K3_001190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9145083.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACG8K3_001191"
     CDS             24539..25840
                     /locus_tag="ACG8K3_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9145084.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACG8K3_001192"
     CDS             complement(25909..26508)
                     /locus_tag="ACG8K3_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9145085.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8081 a         5482 c         4770 g         8298 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt g
//
LOCUS       DAZKCN010000031        26291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000031 DAZKCN010000000
VERSION     DAZKCN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.031"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8K3_001193"
     CDS             complement(<1..339)
                     /locus_tag="ACG8K3_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFP9145086.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKV"
     gene            complement(464..547)
                     /locus_tag="ACG8K3_001194"
     CDS             complement(464..547)
                     /locus_tag="ACG8K3_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9145087.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(596..835)
                     /locus_tag="ACG8K3_001195"
     CDS             complement(596..835)
                     /locus_tag="ACG8K3_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9145088.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDMMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(955..2481)
                     /locus_tag="ACG8K3_001196"
     CDS             complement(955..2481)
                     /locus_tag="ACG8K3_001196"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFP9145089.1"
                     /translation="MAICSKKQVKFRAMNYSGFFEVEASIKVNQGSFKGVLSPTQRFK
                     SQEELLSFVSSKQAKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKATASQ
                     TLKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPRKRY
                     VDGSKVQEHHAIIPTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEETKIQ
                     TKVADLLFQSIGKTPKQEGWKILFKQQTKEEKEDVQTLPLVIIGEHAEVDVKSAEKET
                     QPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQV
                     IKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFINNIKKFI
                     VHLLEAVPNDIEKLNFSDYQEQKEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECK
                     FTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK
                     "
     gene            complement(2633..3952)
                     /locus_tag="ACG8K3_001197"
     CDS             complement(2633..3952)
                     /locus_tag="ACG8K3_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HFP9145090.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
     gene            complement(4180..4707)
                     /locus_tag="ACG8K3_001198"
     CDS             complement(4180..4707)
                     /locus_tag="ACG8K3_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFP9145091.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            complement(4751..5614)
                     /locus_tag="ACG8K3_001199"
     CDS             complement(4751..5614)
                     /locus_tag="ACG8K3_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9145092.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            complement(5647..6381)
                     /locus_tag="ACG8K3_001200"
     CDS             complement(5647..6381)
                     /locus_tag="ACG8K3_001200"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9145093.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(6362..7231)
                     /locus_tag="ACG8K3_001201"
     CDS             complement(6362..7231)
                     /locus_tag="ACG8K3_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9145094.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(7246..7470)
                     /locus_tag="ACG8K3_001202"
     CDS             complement(7246..7470)
                     /locus_tag="ACG8K3_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145095.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(7707..10694)
                     /locus_tag="ACG8K3_001203"
     CDS             complement(7707..10694)
                     /locus_tag="ACG8K3_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP9145096.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            complement(11008..11493)
                     /gene="vanZ-A"
                     /locus_tag="ACG8K3_001204"
     CDS             complement(11008..11493)
                     /gene="vanZ-A"
                     /locus_tag="ACG8K3_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9145097.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(11646..12557)
                     /gene="vanY"
                     /locus_tag="ACG8K3_001205"
     CDS             complement(11646..12557)
                     /gene="vanY"
                     /locus_tag="ACG8K3_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP9145098.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYETWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(12985..13593)
                     /locus_tag="ACG8K3_001206"
     CDS             complement(12985..13593)
                     /locus_tag="ACG8K3_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9145099.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(13599..14630)
                     /gene="vanA"
                     /locus_tag="ACG8K3_001207"
     CDS             complement(13599..14630)
                     /gene="vanA"
                     /locus_tag="ACG8K3_001207"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9145100.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(14623..15591)
                     /locus_tag="ACG8K3_001208"
     CDS             complement(14623..15591)
                     /locus_tag="ACG8K3_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9145101.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            complement(15806..16960)
                     /locus_tag="ACG8K3_001209"
     CDS             complement(15806..16960)
                     /locus_tag="ACG8K3_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9145102.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(16938..17633)
                     /gene="vanR-A"
                     /locus_tag="ACG8K3_001210"
     CDS             complement(16938..17633)
                     /gene="vanR-A"
                     /locus_tag="ACG8K3_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9145103.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(17847..18422)
                     /locus_tag="ACG8K3_001211"
     CDS             complement(17847..18422)
                     /locus_tag="ACG8K3_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9145104.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            18568..21534
                     /locus_tag="ACG8K3_001212"
     CDS             18568..21534
                     /locus_tag="ACG8K3_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP9145105.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            21675..22280
                     /locus_tag="ACG8K3_001213"
                     /pseudo
     CDS             21675..22280
                     /locus_tag="ACG8K3_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(22325..22492)
                     /locus_tag="ACG8K3_001214"
     CDS             complement(22325..22492)
                     /locus_tag="ACG8K3_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9145106.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(22526..22825)
                     /locus_tag="ACG8K3_001215"
     CDS             complement(22526..22825)
                     /locus_tag="ACG8K3_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9145107.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(22866..23483)
                     /locus_tag="ACG8K3_001216"
     CDS             complement(22866..23483)
                     /locus_tag="ACG8K3_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9145108.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(23808..24203)
                     /locus_tag="ACG8K3_001217"
     CDS             complement(23808..24203)
                     /locus_tag="ACG8K3_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145109.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            complement(24283..26115)
                     /locus_tag="ACG8K3_001218"
     CDS             complement(24283..26115)
                     /locus_tag="ACG8K3_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP9145110.1"
                     /translation="MQQNRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEG
                     NSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIK
                     GLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLT
                     TEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFA
                     VDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDM
                     STGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDN
                     YIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDR
                     KIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGW
                     LGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDS
                     TALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFF
                     IDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            26177..>26291
                     /locus_tag="ACG8K3_001219"
     CDS             26177..>26291
                     /locus_tag="ACG8K3_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9145111.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
BASE COUNT         7951 a         5199 c         4339 g         8802 t
ORIGIN      
        1 cactttttta ataatttgtg tgcttaaatg gtaaggaata ttcccaacaa ttttatacct
       61 ctgtttgtta gggaattgaa actgtagaat atcttggtga attaaagtga cacgaatgtt
      121 cagttttaat ttttctgacg ataagttgaa tagatgactg tctaattcaa tagacgttac
      181 ctgtttactt attttagcca gtttcgtcgt taaatgccct ttacctgttc caatttcgta
      241 aacggtatcg gtttctttta aattcaattg ttttattatt tggttgagta ctttttcact
      301 cgttaaaaag ttttgagaat attttatatt tttgttcatg taatcactcc tgaagtgatt
      361 acatctataa ataaatacag aagttaaacg atttgtttgt aattttagtt atctgtttaa
      421 aaagtcataa gattagtcac tggtaggaat taatctaacg tatttattta tctgcgtaat
      481 cactgttttt agtctgtttc aaaacagtag atgttttatc tacattacgc atttggaata
      541 ccaacatgac gaatccctcc ttcttaatta caaattttta gcatctaatt taacttcaat
      601 tcctattata caaaatttta agataatgca ctatcaacac actcttaagt ttgcttctaa
      661 gtcttatttc cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga
      721 tatggtgcaa gtcagcacga acacgaaccg tcttatctcc catcatatct ttttttgcac
      781 tgattggtgt atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact
      841 tctttctatt tcttcttact cttattttat catcacaaat cgcaagtgat ttgtgatttg
      901 tgataggtga tttgtgatta atatataaaa gccctgttta aagggctttt atgtttattt
      961 tgagaaagag ataaaatcaa tatatccctt ttctccgatt tttacaacgg cattgtaggg
     1021 ctttttctct ttgtttttga ttccttttac cagggtttct tttccctcca gtaattcttt
     1081 tacattcgtt ttggttagtt ttttctttct aaaatgttca gctaaagtaa acttacattc
     1141 aggataattt gaacaaccat aaaacgattt ttttaataca atattgttgc cacacttagg
     1201 acattttcct acaatacttt tttcagcttc tttttctttc tgttcctggt aatcagaaaa
     1261 atttagtttt tctatatcgt taggtacagc ttccagtaaa tgaacgataa acttcttgat
     1321 attattaata aagttttctt gattgccttc tcttttaccg atttttttta aatacgtttc
     1381 ccatttagcc gtcatttcag cactcgttaa aaggtgctga ctttcaactg cctggcacaa
     1441 taattttcct ttttcagtta caacaagctt attcttaatc acttggatat attctttttg
     1501 ttttaacgct tcaataatac ttgctcttgt cgcttctgtt ccgatcccct cgacttcttg
     1561 taaaatcttg attgcttctt catcatcaac cgttttatta gcggttttca tagcagttaa
     1621 taatgtaccc tctgtaaaag ctttcggtgg ttgtgtttct ttttcggcac tcttaacgtc
     1681 aacctcggca tgttcgccaa tgataaccaa gggtaacgtt tgaacgtcct ctttttcttc
     1741 tttggtttgt tgtttgaaaa gaattttcca accttcttgt tttggtgtct ttccgatcga
     1801 ttgaaacagc aaatcagcta ctttcgtttg tatcttggtt tcttcataca agtaatcagg
     1861 taaaaacatg gcaacggtcg ttttaacgac taaagcatat atttttcgtt gtaaatcgtc
     1921 catttttgct aatgcagatt cggtaggtac ttgttttgtt gggataatgg cgtggtgttc
     1981 ctgtacctta ctaccgtcca cataacgctt tctaggctct gtttgaacca tttcgagatc
     2041 aagtcctaaa aaaccgctat atttgccaaa atttgctttt aaataagcaa attcgttctc
     2101 tgtaataaat ggtgtatctg ttcttggata actcaataat tttgcttcat acagtccttg
     2161 catagctttt aaagtttggc tcgctgtcgc tttataaagc tgattgactt ttgattgtaa
     2221 actacttaaa gaaaacaaac tcggactatt cgttttcttc tctttggttt gaacatcagc
     2281 aattatcccc tcttgattgc ctattttagc ttgtttagaa gaaacaaagg ataaaagttc
     2341 ctcttggctc ttaaaacgct gtgtggggct tagaacgccc ttaaacgacc cttggtttac
     2401 ttttatacta gcttccacct cgaaaaaacc tgaataattc atagctctaa atttaacctg
     2461 tttttttgaa caaattgcca atttttaaat taggcagata ctttcttcca aaataatgtg
     2521 ttttcgaaca ggatgcctac ggaagaagat cataataaaa attatttgtc tatttaaggg
     2581 cagacgaatc ccttgaaaaa gaatcggtta aaaaatttgt gtgataacta gtctaaatcc
     2641 attgtttgga tcgccgcagg cgttcaaaaa ccttataaaa ttcagtttga tacacatgat
     2701 aacttgacag tttgagatag acctgtctcc cagtctgaac aagtttaccg gctactttaa
     2761 gcaatatcag ccggatggaa tgaatcgtca tgccctggtt cactttatca aaaccaatgc
     2821 tttttaaaaa attaacgagg ttgtatgcaa ggacactgat catcattctg acatgatttt
     2881 ctaagaaacg cgggctatct gttttatcga agtaaaagcc acctttcgct tctttgatga
     2941 aattttccat tgttcctcgc ttgccgtaaa gagaaaatat gacttcaggc gagacattgt
     3001 cggatagatt agtcacaata aacgcatgat gaaatagcaa ttctcctcct tcacggacgg
     3061 agcgtataca gacacgacga ggtttcgacc aggattgtgc ttgataaggt agtgaaaaat
     3121 actgcacttc tcgctcttcc cattcttgat tgtccccgta aagaacagaa tgttcagcta
     3181 actgacttaa cctccgattg ctctttaatc ggataacgta ctggctttca gttgcttcac
     3241 aagactcata cacctctggt gtggcgaagc cgctgtcacc ccgaaccaat atctcggtat
     3301 gaggtaacgt gctcttgtag tggtgtaaaa gcgggttgat aaaggctttt acccctttag
     3361 atgtgtactg attgcctgaa cgcagttcag cttttaagaa atctccggtc agtccatcaa
     3421 aggcaactaa tgggtgatag ccgtaggttt gataatgggc attataatct gtttgttctt
     3481 ggcgtccaaa ggtatccgaa tgtgtggaat cgacatcaat gattaactcg gtgtcattgc
     3541 gaattaaacg tgctttatca atcaatgact gatttaatgc ttggagttga tcgatgttct
     3601 cctcggtgaa acgatccaga aaccgtgaga ttgaggattg tgatgccaac tgttttttac
     3661 cgagtacagc ttgaaagaca ggatcccgtc ttaatagatt agctgatgag tctgccgagt
     3721 aaccagcaat taattgcata ataagctgtt ccaatatcgc taagttatca tgggtaaaat
     3781 aagcccgatg atctgtaata tggagccatt gtttagctaa gtgcgaaaag ccgaaggtat
     3841 tcattaattc tttaaccagg actaaccccg aatcacttga tagacgacca ccggtatgtg
     3901 aaatagtgat atttgaattg aatttaactt gatttttgtg taagctagtc atgagaagaa
     3961 ctcctttctt atggttggtt tcgtcacctt taccatagca gaatggggtt cttttttcat
     4021 cacttaaggg gtgaaactaa aaaagtaatt atcagactgc cgttaggcag tttcagacgg
     4081 tttcaattaa aaagtatgaa ttattcaggg taaacatctt tcgttgtatt gtaaattaac
     4141 gccatccaaa attttgattc aagattttct ttcggtttat tatatgtttg agccaatata
     4201 agctacaaaa ctgctgtcta tccatttttg agtaacctca tttatatcaa tcccaatagt
     4261 agccaaccca ataatgctgc aatccaattt ctctaacaaa gctattaaag ccttcatttg
     4321 agaacctgtc tctatccaat catctacaat caaaactctt ttgttcctca caatagaact
     4381 cttagatatt tcaaagcttt tatcttgacc tgaatagtcg gtaaaatgtg tcgaaataat
     4441 ctcttctttt gcgtatggca gcttatcacc ttttcttact ccaataaacc ccactccaag
     4501 ttctttggct atggcagtgc ctagtatcca tccaattgct tctggggcag ccacataatc
     4561 tactttattt tgaaaatcca atgatagtac ttttacaatt ttatcaaaaa cggttttatg
     4621 tgtaaaaata gttagtaaat catattttcc aattgagtta cgaggtaata tacctctaat
     4681 ttcatttata acatcagtac tttccataca tttacacccc ttgcttatgg ttaataatct
     4741 ttgctaatct ttaaaaatag tcattttcaa catatttttt atacatgtcc tcggtatatc
     4801 ttgttatgtt cttaccatac tctggataat caaaccccag tagttctgct acctctttgg
     4861 acacttccct gaacaattgg tggcatataa ataatgactt ccaaatattt tcataggaat
     4921 ccatgcgata tgtagataat aatctattcc atagatcttc atcaatgtat ttgttaatat
     4981 acttataatt tttcccaaca cttaatgaaa attctgtctt tatcccaacc ttccacgaca
     5041 tcatcctaag tagttcaaac cgtagaatct ggttcagatg atcgattgca aacagtatct
     5101 ctttgcggca caatccttta ataacataag gtgttacatt ccaaaattca ttgcagcaat
     5161 catcatactc ccttgcgctt ggctttctta catgataatc tatatcagtc ggaactatgt
     5221 cccttttaat tctacaatct ttatcaatta gaacctttat taatttatcg ccctttaggt
     5281 aattatctaa ctcttccaag ggcaataagg taagatcaat tttattgtaa tcatcaaata
     5341 gcataagata ggaaaatccc ttttcttcag gtgggaataa ttccatatcc tccggctttt
     5401 gcatcattat tatattccca aattgattaa gccagtcatc attagatata aacggttcta
     5461 tatcacttac aaaatatgta atatcataat cctgaaattc atctttaggt atattaatat
     5521 ttgcgcgtga cccctcaagg gtcacaattc gaatacgttc atcctgttct gctaaagaaa
     5581 gtactaaatc catcatttct ttttctgatc tcatttacat actcctcgtt tattttttct
     5641 atattattac atcttctttt ttgccgatac aatcagcatc attgggcgtc gcatttcatc
     5701 cgccatcccc ggaatatcca tcatgttctc tggcggctgt ggctccacaa tctgatttat
     5761 tataaaacta tttgaaagca gtgtatttag atatgtggtc agtgttctat gatattttgt
     5821 aaccttttct tccaaaaaca tagctgtccg tttgccctca taataataat tgtccaccgg
     5881 gaaatgcagt atttctcctt tttcgttata ataccagtct tgtgttccat gagcagtaaa
     5941 aacaggatgt tcaactgtaa aaactaaatt gccaccagcc ttcagcatcc tatatatctt
     6001 ttttattaaa ttctcatagt ctgctacata atgaaacgca agcgaactta gtattacatc
     6061 aaagctctcc tctgggaaat ccacatcttc tatggcacag cattcatatt caatctgtgg
     6121 aaaatgggtt tttccttttg ctacttcgag cattttatga gaaatatcaa cacctactac
     6181 agaggaagca ccgttttcca tcgcatatat acagtgccat ccatagccgc atcctaaatc
     6241 aagcacacgc ttacccttaa aatcaggtag catctttttc aaagtctccc attctcccgc
     6301 accagccagt cctttctgcg agcgactcat ttgactgtat ttttgaaaaa atatattatc
     6361 atcatatttg ttttctttca tctgaactct cctcgtttaa aaagttattt atctccgata
     6421 caatttcatt cacttcttta taaagtttct cggtcatatc gtagcattca aaaagagaaa
     6481 gaccgagtac ttcaaaaata tgattaactc tttcggcata tttttcaggt ttatattcaa
     6541 aagtttcaag cagttttata gctttctttt cgttaatgca ataagcatta ttacatgcaa
     6601 atagtacttg atttaagcat gaaataatac gaaaaacatg gcctgcaata taatatttat
     6661 cctctacccc cgcatttgct tttacaaaca ttaaagagaa ttcagcttca aatataaaaa
     6721 agttcatcaa gcttttcttt agagcagtag ggtaaatttc tgcctgcttt tttaattcgc
     6781 ataagctttc gttcttagca tatagtattt tactaatcgc taattctcca cgatacattg
     6841 cacttatata accatgggga tgcccggtct gataattggc agtaacaatt ccttgctccg
     6901 tatctttgat tatttgttct acccgtttta tatcacgtaa aatcaagtca acatgatacc
     6961 catttataaa taaccatccg ccgccattaa tccaatcacc ccacgctccg ggaggcacaa
     7021 caaggttgtt tctattttta tcatccaatt ctgtagcaat ttgattaatc gctgtcaggt
     7081 caaatgagtc tgaattgtaa tagattccga tatctatatc agaattttct gtatgggtgc
     7141 ctcttgcacg tgagccccct aatacgatgc cttctataaa aggcaaagat gataatttct
     7201 ctgttactat ttgaataaca ttacctacca tataaacatc ctcctttatt cgttagatat
     7261 ttttatttct tctccatctg gtaaaataaa tgcctgcaca aacttgacac ctagcacatc
     7321 agcaatggcc ttcaactcat ccaaggttac tgtttctcgt tgtaattttt tattgaaatt
     7381 ctgtggagtc tggccaatac gtctagcaag ttcggaaaca cttatattca tttgctcaca
     7441 cagttcttta atcatttctg ccgtagtcat acagcacctc caagtttacg ctaagaatat
     7501 tataaaccat ttggttgata aacacaatag ttattaaaat atggggtaca tggaacatag
     7561 aaacgaaata gtgcggtagc acaaaagtaa aaacacaaga aacgttgatt taacgctgtt
     7621 ctcgtgtttt tctctttttc tattcgaagt gattcgctgg aacagtcata cgaaatagtg
     7681 cggtagcatg caaaacccga acattgctag aattcttcta cattcaatgg acgtaactta
     7741 ccgttggata attcagcttc gtatctaaac gtatacttac caacaaaatt gatgtgatca
     7801 ctaattagtg gagattgcct gcgtatcatt tcttcggaac agtcgaatcc ttcagctttc
     7861 atttgttcaa atactttttc taaatacagt gagttccaat atatgatggc attagtaact
     7921 aatcctaatg cattgagctg ctcttccatc ccatcataat aagtctggta gagttttcct
     7981 tttcgtccat aaaaaatatt acggcataag gcatgccgag cttccccctt attcaattgt
     8041 tctaaaattt gccgtgcata tatttcgtca gaaatataac gaagctgatg tttcgtctta
     8101 taaacttttc catactcagt tattgctttt cctaaaggcg tgggttgccc attccgttga
     8161 agagatttcg ttaattctgt tgcattcact tttccagatt tcaacgaacc tgcaacccta
     8221 agaatgtcct cccaatgggt ataaatcagt tccatattaa ttgaattttg agacaacgta
     8281 tcaagttccc cgtagtgagc gttagcgtcg attcgccaaa gtttggttcc tttatcattg
     8341 gcaattcttg gactaaattg aaaccctaat aagccaaaga gaccaaaaat catatcgcta
     8401 taaccagctg tatcagtcat gatctgatta ggagttaatt cgctgggttg attgaggaat
     8461 ccttctagaa ggtaaagcga atctcgcagt gtccctgaaa cgaccattcc gtggaaccct
     8521 atatattgat cagagacaaa attgtagaat gtgatcccac gaccacggcc aaaatatttt
     8581 ggattacttc tagaatatag ggaacgttgt ggtgtgatat aacgaatacc gtcagctgaa
     8641 gccatatgcc catcacccca tatcttagag gtggcaagtt tcctgtgtgc atgtattatt
     8701 ttttgattag ctcctgaaag tgtatccact cgaatataat gatgattgac atagacaaga
     8761 cgatcatact ttaaagaatc aaatccttct tttgaaaccg gggaaaaacc aatattgcag
     8821 gactctgata aaagaactgc taataaacta atatccaagt ctttcatttt ggtgtccttg
     8881 tcacttaagt ggttaaatga ttgagtaaga tttaactgtt ggtttacttc caataacaaa
     8941 tctgacaaat caattttagg aagtaactgc tgaatgcgac gtctaaaaga cttatcttcc
     9001 tttttttcat atgtcttttt aagtctagag acaaccaggc gatctgcatt ttctattctg
     9061 gccattttag aagtaggcca acgatttagc gcttctttat aagacagttc caaatcgttc
     9121 tttaaccata aagacatttc atttgctgaa gaaggaagat ctagttgttc taataaaacg
     9181 tatttttgtt gaacccaagt tttctcatca atcaagcaat ccataggatt gttatataag
     9241 acactctgag gaatataaat atcatggcgt tttaaagatt gaattaacaa ttcgacagct
     9301 gcaatcaata cacatttatt agtttgttca gggtattcat gaatgtaata aatccatttc
     9361 tttggaagag tatgtttaat agctccaaac attttgtaag taatcggctt aggaaatcta
     9421 ttattaatga aggtccagag ttccttacag ttgttaccgt agtcattgct gtcaaatgtt
     9481 agtgtagtta aaatatccgg aataaatttt ctgaactttc gataagtgtt cagaagttca
     9541 gaaatggcta ttggttcacg atcattttta accaatagcc gaacttgcga tacagcttcg
     9601 gtaacatttt cctcagaata tttttctaag atacgatcgc gaagggatag ttcattagta
     9661 tcagaatgac tggacattac taaatctaca atatctgaaa gtgtcgatgc ggctcgatct
     9721 agatctttta tggtacgaag acgttcttta gattccttat ttttggcgcg tctaaaaaca
     9781 gtttcaaaaa aattagatag tgctaaaatt tgttcatcca tagccttacg tttgtatatg
     9841 aaaataaatg ccactaacaa cgccagtttt ttctgttcac tcattcgttg aattaattga
     9901 gctttagctt taaatgcata atttgcaaga acttttattt tcccttcagg aatagattcc
     9961 atcttccatt tttcagtaga gaaagcttga aattgcttta gtcggtcaaa acctctggct
    10021 atttctttat gactatcgtc agctaacggg gttctcaaaa tgtccatttt gattattgcc
    10081 ccataaactg gagttccaat taattctaaa tgattgagaa gccgctttct ctcatcttca
    10141 gttggaacca aagacaattg tcggtataaa tgctcttcag ctttttcaat tgcactagca
    10201 acaaaacgag aaaaagtagt aattccaggt aaaacaatct tttcatccag acattttttt
    10261 agaagcatat caaacaacat atctgaagat tctgttgtat acagcgctct atttaaaagc
    10321 cagttagcta ggtatgtttc tatttccggt tggttgaaat cttgatatcc ataatgtgat
    10381 tttatcaatt ttgtgtgctg atacctggtc tgtctcctgc tatagtagat aatttctcca
    10441 ggatcaatat ttagttgagt tgctacataa ctcaatacat tttggggaat attggtaaga
    10501 tctaatataa aagttcctaa aaaacgtagt gagactaact gtagtgcaaa acctaatcta
    10561 gtagctggtc gtcgtatagt ctccatcaat tctaaatctt tttcatgcaa ataaaaaaat
    10621 tggtttaatt gttctgtact aggttctcct aaataaatgc caaatgcttt tttgttactt
    10681 ttgatttcat ccatttaaac cctccatgaa gtaaatttta agtctacata aaatttattt
    10741 ttaaaaatgc tatgataggg gtaccgtcag gaaaatgcgg atttacaacg ttaaggaaat
    10801 ttattaaaga gaccaattcc tcagcactaa aattgataat tgggctttct ttgttactat
    10861 agtgttaggt aagcttttta ctttatcacc ggttttcccc tcacttcaca cctacgggcg
    10921 agtttcccca catcccgttt tccatctaat ccaattcatt caatatcttt atgtgtatga
    10981 aagatcagat tgcttgattt agacatctta cacgtaattt attcttaaat gggtacggta
    11041 aacgagcaat aatacgagca aaagtatacc tacaaaaata ataactctgt ctaatttttt
    11101 ttgattcata tgcttattgc ttaaaccata taatttcagt ccaagaaagc ctccaacagt
    11161 atttgtaatt acatctgtta tgtctgtcgc tccaatagcg aagataaatt gaattatttc
    11221 aaaagtaaga cttaaaacca gtacaaaagc aaacttaggt aaaaatccga tttctttaaa
    11281 attgacattc aaaagcaagc caaatggaat aaagattata acattatcta tcatctctct
    11341 gaaattccca gtagcagtaa atggagtcaa gttaagactt ctttgatgat aattaaatac
    11401 tgataaaata ttgtattgta atttgaataa cactaaccag attagtgtca ctaaatataa
    11461 agctagcaat cctctagata atatttttcc caatataatt tcctctaatt cttttgttta
    11521 agttgtatca ttagtatagc aatgtcctag ttaacgtggg ttttaaatta aaaagctaac
    11581 gttaccccaa aagtgctagt acaataatgc tgttcattaa tttcgttggt ttcattccgc
    11641 catccttacc tccttgaatt agtatgtgtt gatccgggaa acactgtcac aattacaccg
    11701 tctatattgt ttcctgatat ctcataacga agattagtcg gcacatgaat ggtggtattt
    11761 ttagtaacag gataataaaa gatctcatat ttttccccat ttacactaac agaaatggtt
    11821 ttttcttctt ttaggtaatc catatattcc tcgagaacga aattcttttc tttcataatc
    11881 gcactatgtg gtaaaccaac atagcgaata tgccatgttt catattgaat tcctgttaac
    11941 tctgttttgt cctctggata acgtaaaatg aacccgtatt tccaagcatt ttcttctatc
    12001 cactttcctt caggggctcg ttccattttc gtcaagcttg atcctacatc tagtgataaa
    12061 cctgaattat gctcactata acctgctggt aaggcatact cagcccccat ttcttggtaa
    12121 agcacacttt gctcatcaaa gtctcgatag ccactattaa taataaaatg actaacgcca
    12181 ccctttacag catcattgac catctctgaa aatttttgtg ctatttcttt tgacatataa
    12241 atattactat caagcaaccc gtatccattt attaattcgt catgtttaga taaattcacg
    12301 atatctgact tcacactttc ttggcgaaca ggatatttac tattgattaa tagcagattt
    12361 ccttgataaa cctgttcttc tgtaattgtt ttctcttggg tattttcaga agtcccacta
    12421 ttttctaaat gttctttggg attttgatca taattttgaa attcgacttt ttcctgagaa
    12481 aacagtgctt cattaacgta gtcataacct aagtatatta agaataacaa taacaataaa
    12541 aaaaacaact tcttcatttt cagtctcctc cttaactata tagttggaag tatgaaatat
    12601 ttctagaatc ttccccttaa acggcacaat ctcgtttgag attgtgccgt tttgccccgt
    12661 aaattacagt caattatttc gccctactgg atttactatt atcaccaatg tagatgaatt
    12721 ttcataagtg atacccatgc tgaatttaac gaggctgaac tgcctgttga caatgggcgg
    12781 tgtgaaatac cacccctcta tgcgatcagt cttattatca ggctatcttg ccgcagcgca
    12841 cgcagataat gaccgcaata tcgctctgcc ttacatcttc aatactatct actatatata
    12901 aattccctaa gaaccagtta catacgtcgg gtttcctgcc gaaagagtta gcttatatag
    12961 tttgtccgtg caacggttaa aagtttattt aacggggaaa tcaaaatagc tattggggta
    13021 tggttcgtct cttaatacat agtgccacca ttcgaggcta tatgcttcaa acccactgtt
    13081 ttccatgatg gagcgcaaac gtctgcgatt ttgcgcttca ttgcatgata ttccatttgc
    13141 cgcatgatga gagcgttcat ccataaaatc aaatcggctc cccattggta caagctcacc
    13201 cgtgtctaat cgataaagcg taagatcaat ggcactgccg cggctatggc ttgattttga
    13261 agccacgtat ccttttgaaa tcatctcagt tcggtcaata ttgggataat aactttcctt
    13321 tgtcaggtta ttttccggct gtgcagccca ttgcataaaa cagtttacag cacgcttagg
    13381 acggtaaccg tcccatagaa gcaatccgta cccttgggta gcagccagtt cttttgcctt
    13441 caaaagcgat tcagccaact cgtatgtccc tacaatgcga tttacttcat aaccgtcaac
    13501 cggttttccg gtgaaattat cccaagtggc atatttagcg tcccaacgaa caccgtgtac
    13561 tatttcatct aaaaaagtaa atcctatttc catgcttatc acccctttaa cgctaatacg
    13621 atcaagcggt caatcagttc gggaagtgca atacctgcag cggccatcat acggggataa
    13681 cgactgtatg acgtgaaacc gggcagagta ttgacttcgt tcagtacaat gcggccgtta
    13741 tcttgtaaaa acatatccac acgggctaga cctctacagc cgagcgcttt atatattttt
    13801 tttgccgttt cctgtatccg tcctcgctcc tctgctgaaa ggtctgcggg aacggttata
    13861 actgcgtttt cagagccttt ttccggctcg acttcctgat gaatacgaaa gattccgtac
    13921 tgcagcctga tttggtccac ctcgccaaca actaacgcgg cactgtttcc caataccgca
    13981 caaccgacct cacagcccga aacagcctgc tcaattaaga ttttgctgtc atattgtctt
    14041 gccgattcaa ttgcgtagtc caattcgtcc gcgctattga cttttttcac accgaaggat
    14101 gagcctgaac gcgccggctt aacaaaaaca ggataggtaa acgtagctgc caccggccta
    14161 tcatctttat taataaccca aaaggcggga gtagctatcc cagcattttt cgcaacgatg
    14221 tatgtcaacg atttgtccat acaaattgct gagctttgaa tatcgcagcc tacaaaaggg
    14281 ataccggaca attcaaacag accttgtatg gatccatctt cacctgactt gccatgcaaa
    14341 gctgaaaatg ctacatcaac atggttgatt tcatattcat ggttcttttt aacaagtaat
    14401 ccgtgcattt ttttatccgg cgagagtaca gctgaatagc aattgtcgtt ttcccattcc
    14461 gcgcaaggtt tttcgcacat tttccataca ccagatttcg taattccaat gtataacggc
    14521 tcgtattttt ctttattaat gttagcggct atctctattg cagattttac cgatacgtca
    14581 tgctcctctg agcaaccccc aaacagtatt gcaactttta ttctattcat gctcctgtct
    14641 cctttcaaaa tccaaacagt ttttaatggt tttttcaacg gtatcacgca acgcttgctc
    14701 ggtataatag gccgtatgcg gtgtgattat cacgttaggc attctttgaa gtttaagtaa
    14761 aaattgatta tcaattggtt tttgggtgca atcagagtag aaaaactctt cctctccttc
    14821 caatacatcc aatgcggcac cgcccagttt cccgttttct aatgctttaa ccaactcata
    14881 ggtatctaca agtggaccgc gcccagtatt gataagaaat gctccttgct tcattctctg
    14941 tatttgttcg tggctgataa tatagtgcgt atccgtattg agcggcacat gaagcgtaac
    15001 gatatcgcta ttttgcagca actcatcaaa cggtacatag tttacctcta tacttcggct
    15061 gcgactataa gccaacactt tacatccaaa tcctcgcagc cgctcaataa ccgctttgcc
    15121 tatctggccc gttcccacca caccaactgt catgtcgctg agtaccttgc cacggtcgct
    15181 gtccaacctg aaatcatgtt tttccacaga gcgcacaatc gattttacgt tgcgtactgc
    15241 cataagaatt agcatcatag tataatcggc aacgctatcc ggcgagtacg ccacattgtc
    15301 gacagtgatg cccattctct tagcagcagt tgtatctata tgattgcagc cgatgcttcg
    15361 ggtagaaata tatttcacac cggctctctt cagcgcaaga agaatagagg cggaaatctc
    15421 tgatttatgt cccacactga tacattgatt gaaaggcgcg gatttggcgt tggattccga
    15481 cacgttggcg ttaattatcg ttgccataac gccaaagcga ggcgaaagag catggaatgc
    15541 atctgcctca tcctgctcac atccataaac agtaatgccg atgttattca taattaagac
    15601 caaccctttc tgtgaaaggc accaaataaa acggggatgc caatggtaat cttgtcaaat
    15661 ctttctctga agaacgaaaa cggctcgttc ttacggtatt aagtctaccg tgtaagaacg
    15721 agccgtttct taatattaaa ttgttaattt cctaagaaaa agtaaagata accttgagat
    15781 tttcctaaaa aattatatac atctcttagg acctcctttt atcaaccaag tctggcatcg
    15841 ctggaagctc taccctaaac gtcgtatagt tatcattgct ttccgcgtaa atctgccctc
    15901 catgctgaac aataatttct tttgcaatcg ccaatccaag tcccgcgcca cccgtatcgg
    15961 aagaacgagc attgtccagc ctatagaact tttcaaatat ggcagctagc ttatcttttg
    16021 ggatgcttcc agtgttcttg aattcgattg acaccacatc cccggagagg cccgcggtaa
    16081 tgtcaatgat gctgttatcc tcactgtatg cagcggcgtt tttcaaaatg ttgttaaaga
    16141 ctctcgcgag tttatcaggg tcgccggaca cggtcagatc ctcgggggcg tgaataaccg
    16201 cctgttttcc atgtgcggaa agctgaggat aaaattcatc ggtcatctgc accagcatat
    16261 agtataggtc tatgtgcgtt tttgttagcg ttatcgtttg taggttatac cgtgtaatct
    16321 caaaaaactc gtcgattagc tgttcgagtc gatacgcttt gtccaacgtg atatgcacat
    16381 actttgcctt ttgatctacc ggcatgtctg gagcctcgtc aagcaggctc aaataaccga
    16441 taatggatgt aaggggcgtt ttaatatcgt gcgccaagta cataacaacg tcattttttc
    16501 tttgttcggc cagctttgca tcctgctctc gcttttccag agtccgtttt aatgtgttga
    16561 gcttttgttc cataacatcc atttccgcag aaagctcaat ttgtttatct tcgttctgaa
    16621 taagtacatc aatgccggta tttatctcgt caaagtattt tgcgaatttt gaaagcatga
    16681 cgcgacatag aataagaata ctaatgacaa tcgccacata aataaagata tctatattgt
    16741 tccgtatgga atattgatat aatttcatcg cgtccaggtg atttaagtca tatttgtttt
    16801 ccaaaatact taagatccaa tccccaagtt tccctcggat cattgaacga atatacaaca
    16861 cgaatacaat tgctaccaca acaattgcaa cgatatacat gtaaagtttt cgttctagtt
    16921 tggaatagtc gtttttttta tttttcaatt ttataaccaa ccccccatac cgtttttata
    16981 tatttcggat tatcaatggt gtcgttcatt ttttcgcgca aatgccggat atgcacggtg
    17041 atggtgttgt tgctcttgct gaaatattcg tcgccccata tctcatgaaa tagcagctcg
    17101 gagctaacca cattcccctt gttttcacag aggattcgca gtattgaaaa ctcggtggga
    17161 gtaagggata actgcttctc gttcagataa cactcatggg tgttaacatt aatgacaagg
    17221 ccggagtgga cgataacatt ttcgttctgc tcctttactc cactgaattt tttgtatcgg
    17281 cgcaactggg cctttacccg agcaattaac tccagtgggc gaaagggctt cgttatataa
    17341 tcatccgcgc cgattgttaa ccctgtaatt ttatctacct ctgtatcttt cccggtcagc
    17401 atgataatcg gataggtgtg cttgtccctt attttttgac agatagtaag gccgcttgtg
    17461 ccgggaagca tgatgtccaa tatggcaagg tcaatctcag acttgtctat acattccaat
    17521 gcttctttgg cggtatagta tttgaaaacc gtataattct cgttttttaa gtataattca
    17581 accaaatcgg caatttcatg ttcatcatcc acaataagta ttttatcgct catagttatc
    17641 accctttcac ataagtttgc ccttaataat ttatcagatt atagggccga atttataagc
    17701 atttacataa taaatgacta agaatttctt aagatgatga ttcttttttt atcaaagggc
    17761 ttttcggcat cgctaggagg ctgttaatat tttatagttt ccttttcttc tttaaaaata
    17821 ttgcccatta gcggaataca gaatggctaa ttattcactt ctgataattt cctgtataat
    17881 gaagccctag atacattagt aatttcacaa atttgattta cagtcatatt tccttcttta
    17941 tatagcttta ccgcataatt cattcctgcg tgatttttat gatacttctt taatcgacct
    18001 ttaaactttc cttctttctt agccaattca atcccttcac gttgtctcat ccgaataaga
    18061 tctcgctcta attggttaac accagccatt acagtaatta agaattggct gtatggatta
    18121 tcttctgata aatcaagcca tgtatctttt agtgatttta aacttgcctt tttatctcgt
    18181 atgttatcga ttaattcaaa tagatcttgt gtactacgag tgattcgagt taagtctgta
    18241 acataaatga tgtcatcttc ctgtaaatcg tctaacactt tttgaagttg ctcgcgatcc
    18301 tttgttgctc ctgaaacttt ctcttcatat ataatatcca ttccgatctc gttcaactgc
    18361 tgaaattgtc ttgaaggatt ctggttagtc gaactgacac gaatataacc gattttccgc
    18421 aaaatatcac ctcatttttg agacaagtct tatgagacgc tcttaactat gattttatca
    18481 gtctactaca tttgtatcaa tagagtacac tctattgata tataattgaa ctaataaatt
    18541 gaaaatacag aaatggaatg atactgaatg aaaattgcga gaggtagaga attgcttaca
    18601 ccggaacaga gacaggcttt tatgcaaatc cctgaagatg aatggatact ggggacctac
    18661 ttcacttttt ccaaacggga tttagaaata gttaataagc gaaggaggga agaaaaccgt
    18721 ttaggatttg ctgttcaatt agctgttctt cggtatcccg gttggccata cactcatatc
    18781 aaaagcatcc cagattcggt catacaatat atatcgaaac agattggtgt tagtccatcc
    18841 tcgcttgatc attatcctca aagggaaaat acactttggg atcatttgaa agaaattcga
    18901 agtgaatacg actttgtaac ttttaccctg agtgaatatc gaatgacatt taagtacctt
    18961 catcaattag ctttggaaaa tggtgatgcc attcatctac tgcatgaatg catagatttt
    19021 ctaagaaaaa acaaaattat actgcctgct atcactacac ttgaaagaat ggtgtgggaa
    19081 gcaagggcaa tggctgaaaa gaagctattt aatacggtta gtaaatctct aacaaatgag
    19141 caaaaagaaa agcttgaagg gattattacc tcgcagcatc catccgaatc caataaaacg
    19201 atattgggtt ggttaaaaga gccaccgggt catccttcac ccgaaacttt tctaaaaata
    19261 atagaacgac tcgaatacat acgaggaatg gatttagaaa cagtgcaaat tagtcatttg
    19321 caccgtaacc gcctgttgca gctgtctcgc ttaggctcaa gatacgagcc gtatgcattc
    19381 cgtgactttc aagaaaataa acgttattcg atattaacca tctatttatt acaacttact
    19441 caggagctaa cggataaagc gtttgaaatt catgataggc aaatacttag tttgttatca
    19501 aaaggtcgta aggctcaaga ggaaatccag aaacaaaacg gtaaaaagct aaatgagaaa
    19561 gttatacact ttacgaacat cggacaagca ttaattaaag caagagagga aaaattagac
    19621 gtttttaagg ttttagaatc ggttattgaa tggaatacct ttgtctcttc agtagaagag
    19681 gctcaggaac ttgcacgtcc tgccgactat gattatttag acttactgca aaaacggttt
    19741 tattcactaa gaaaatatac gccaacgcta ttaagagtat tggaatttca ttctacaaag
    19801 gcaaatgagc cacttttaca agctgttgag attatccgag gaatgaacga atctggaaag
    19861 cgaaaagtgc ctgatgactc acctgtggat tttatttcaa aacgatggaa aagacattta
    19921 tacgaggatg atggtacaac aattaatcgt cattactatg aaatggctgt tttaacagaa
    19981 cttcgggagc atgttcgggc aggagatgtt tccattgttg gcagcagaca atatagggat
    20041 tttgaggaat atttgttttc ggaagataca tggaatcaat cgaaggggaa tacgagatta
    20101 tcagttagtt tatcattcga agattatata acggagagaa ccagcagctt taatgaaagg
    20161 ttaaagtggt tagctgccaa ttccaataag ttagatgggg tttctcttga aaaaggaaag
    20221 ctatcacttg cacgcttaga aaaagatgtt ccagaagaag caaaaaaatt tagtgcaagc
    20281 ctttatcaga tgctaccaag aataaaatta actgatttac tcatggatgt ggcccatata
    20341 acaggatttc atgagcaatt cactcatgct tccaataatc gaaaaccaga taaggaagaa
    20401 acaatcatta tcatggctgc ccttttagga atgggaatga atattggctt gagcaagatg
    20461 gccgaagcca cacccggact tacatataag caactagcca atgtatctca atggcgcatg
    20521 tatgaagatg ccatgaataa agcccaagcc atattagtaa actttcatca taaattacaa
    20581 ttgcctttct attggggcga cggtacaaca tcttcgtcag atggtatgag aatgcagcta
    20641 ggtgtttcat cactacatgc agatgcaaat ccacattatg gaactggaaa aggagccacc
    20701 atctaccgat ttacaagtga tcaattctct tcttactaca caaagattat tcatactaat
    20761 tcaagagatg cgattcatgt tttggatggt ttgttacatc atgagacgga tctaaacata
    20821 gaggaacatt atacagacac tgccggttac actgaccaaa tattcggact gactcattta
    20881 ttaggattta aatttgcccc aagaataagg gatttatcgg actcaaaatt atttacgata
    20941 gataaagcaa gtgagtatcc aaaactagaa gccattttac gtggacaaat aaatacaaag
    21001 gtcattaaag aaaattatga ggatgttttg cgattagctc attctataag ggagggaaca
    21061 gtttcagcat cccttattat ggggaagcta ggttcctatt caagacaaaa cagcttagct
    21121 acagccttac gtgagatggg ccgaatagaa aaaacgatct ttattttgaa ttatatatcg
    21181 gatgaatcat taagaagaaa aatacaaaga ggattgaata aaggagaagc catgaatgga
    21241 ttggcaagag ctattttctt cggaaaacaa ggtgagctta gagaacgcac catacagcat
    21301 caattgcaaa gagccagtgc tttaaacata attatcaatg ctataagtat ttggaatact
    21361 ctccacctaa caacagcagt tgaatataaa aaacggacag gtagctttaa tgaagatttg
    21421 ttacaccata tgtcgccctt aggttgggaa catattaatt tactaggaga ataccatttt
    21481 aactcagaga aagtagtctc attaaattct ttaagaccac taaaactttc ttaacgttgt
    21541 taaaaacgag ggattcgtca ggaaaatagg cttagcgttg taaatccgca ttttcctgac
    21601 gctacccctg atagcagtaa aactacaagt aaattttaca tgtaaattat atcactttta
    21661 aaaaggagtt ttttatggga aaaattggct atattcgagt atcttcacaa gatcaaaatt
    21721 tggatcgaca attggcaatg atggaaggac ttaacattga taaattattc caagaaaaag
    21781 ccagtggtaa agatactgag cgtcctgagt ttcaaaaatt actaaattat atccgtgaag
    21841 gtgattgcgt catcgttaca tcactagatc gtttaggaag agattatgaa gatataaaaa
    21901 atacagttgc ttttatgaaa cagaaaaaag tagcgttaaa aatattagat gcacagtttt
    21961 tagattttaa tactggaaat gaactcctgg ataccgctat gttcgatatg tttttatcac
    22021 tgctaagtta tattgcacaa aacgagcgtg agaaaattcg tgaacgtcag cggtaaggag
    22081 tattattggc tcaaaaagcc ggccgatata agggacgacc aatcgagtat tctattaact
    22141 caacagatcc gcaaaaacgc ttagttttta aaaccgtggt ggacatgtta aagcaaggca
    22201 ttccagttgc agagattgca aaagaaaacg ggatctctcg accaactatt tataaaataa
    22261 agaaagcgaa tgatttgtag aaatatacta cattcattcg cttttctacc gcactatttc
    22321 gtttctatgt tccatgtacc ccgtatcggc gatggcattt aggacagtaa aagtaatttt
    22381 tttgataaac actttttgct aattccccac agtcacactc cacgtgaaaa gcaatatttc
    22441 gattaaaact agttatttca atgccccaac gatcataaga ttttcgcttc ataaaaattt
    22501 tcctccttgt aaattttact aacgattata aataccgttg ccattctttc aactgttgac
    22561 taattaacgc ataaaattcg acttctgata aaatggtaat ctcatgacct aacgcaatca
    22621 agtcccgcgc ggtttgttcc ttttttgtat agagagaatc atcaaacaac gacttagaat
    22681 actgaccaca aaccaggaaa tttgtttttg aagtaacact tgattgacaa tggccgttta
    22741 aagagtgaac caaaccgatg gcttgggttc gtgtcatttg ctgcagcgtt ccagtaaaaa
    22801 cgaccctttg attcgttaag tccattttca aactctccct tcccgtctaa tagctgttat
    22861 atctatcaag aaaagtggtg caaggtatgt tcatattctt tcacccgtcg ataaaaggta
    22921 tttcgtttca tgcccaattg atccatagca tagactgcag ttatctttcc tgctttccag
    22981 tcagaataga tcgcaggaaa ttcaggaggt aaagcaattt tctttctgcc gaattttaca
    23041 cccttttgtt tcgcagcttc tacgccttct ctttgccggc tattaattcg tttccgctcg
    23101 tcttcggcca tatagctgag taattgcaga attaagtcag agataagcgt ctctaaccca
    23161 tctagttgct tgtaactagc agtatctaac agaggcatgt ctaacacaga aatatcaatc
    23221 tgcttttcct tagttaattc tcgccattct tctaaaatca tttgtttatt tctgcctagt
    23281 ctatctaatg attgtagata gagtaaatct ccttttcgaa acatccgttt cattaattga
    23341 tattcagggc gtttaaaatc tttgccgctg gctttttcta caaatatata ttcttcaggg
    23401 attcctaatt ccttcgcgga aatcagttgt cgttcgatcg attgatcata actactgacc
    23461 cggatataaa aaaattttct catcgttttt atctaccctt ttttcactaa aattgtttca
    23521 aaacaatcag attaaatcag aaacgtttca aattgtatta tgtggttatt atgccataca
    23581 taaacaaaga aaatcaaggc atgtcaaaaa gtatactttt tgaaacagta agtttacact
    23641 cccgcatgat tatatttatt ccgctaaaat acataaaaat ggcaggcctt aacggtctgc
    23701 cattaaataa actagttatc cagtttttct tttatgtctt ccatggtata ttttattttt
    23761 cacatcagaa tttgacttct ttaactttta caattttttt aacactatta ttctattaca
    23821 acactatata tttctaattt agggttaaaa ataaatcttt ttgatacttt tgcctttaca
    23881 atataattgt attctttctt tttttctttg aagtcgcttg attcaactgt aacttcaaac
    23941 cacatttcat ttggagactc ttttaatttt tttacttgaa atgaattatc tcttggatca
    24001 taaggtggta ctctttcaaa ctcaggaagt tttctctgta tttgttcttc aggagtattc
    24061 gcaaagtaat ctactatctt gacagttatt aaaagtacta ctattcccaa aactataaca
    24121 aacataaaga taattccaca tccagaatta tttttattat tttgagtcag attattccat
    24181 tctatttctt ccataatcat catgtccttt tacaattcat atacttattt tatcgtatat
    24241 atttaaaaag gaaaaggaca ctgtttaaat agtaaacgat tcttaattaa gtttacttct
    24301 attttcttga aattgaataa ttttttcaga aaatccattt gcaactttcc cctcatctcc
    24361 aataaaatca agataagtaa aatctgtccc aagtgaatta ttaactattt tatcaatgaa
    24421 aaatgaaatt atccctacac ctaagatatc aactgtttcg tcattttcta ataaactatt
    24481 tgttactgaa gcgattttat ctgaagaacc aaatctgttc ttaacaaatt gattaaatct
    24541 atcaatagca cccttatcac aatagtacgt ttttaaagcc gataaagctg ttgaatcaga
    24601 atttcttttt gctaaggtat aaacatttac cgcgtcaaca tcttggtata aatcttctag
    24661 actaaatgtt ccacctgcag ccgtacctac taatgcaaat acaccttgct ccatttgtat
    24721 tcctaatttc ttaacttcac cccagcaagt cattaaatct cctaaccaac ctgcaaagtc
    24781 agaaacacta tcattgattg tgtcggctag aaacatctgt gattgtaacg tcacaattaa
    24841 gtgaggcaaa cctattattg aaccagtatt aggatctata aaataattat acaggccctc
    24901 gctattagtt aatttattcc tatattcatc ataaattttt ctatcatgct ctgttacagg
    24961 agaagaaatg atgtcccatg acaaaccaga ataatttaca tatctataaa atgtcaaatg
    25021 agggttccaa ggattatcaa gaccagcttg tgatagatat tgatacgcac gtaccatctg
    25081 atttataacc ttctgattgt atttggcaat gtaattatca ttattagtat catttgataa
    25141 ctcattaaat ccgctatctc tacccgatgt agcgattaaa tcgataccaa cagctccgtt
    25201 gccacttcct atggtaaatt caacaaattg atcaaaagac cattgtctag gcataggaaa
    25261 acctaaattc ccactataac cagtcgacat atctgaaaca aatgcaaacg aagcataccc
    25321 gttattgatt atttgagaac aaacatttct tgttccgtaa acacccgttt gatacccgct
    25381 atatgacatt acagcagata cccccttaaa atattctcca gcatatgcaa gaatttcttc
    25441 ccctaacatg tctagatcaa cagcaaaata aataactgtt gttaatggta tatttagatt
    25501 ttttgctgca tctatagcct tcttaccatc tgataagcca cgatcataag taaaataagc
    25561 taatgctgca cctccatctt gatagatagg aaacacagat aaatttttag aaaaaatatt
    25621 attcaattcc tctgtagtta gatacttatt tcgttcatct tttccaattc caacagtacc
    25681 agtaagatat ctaccaacta tttttatatc attatcaacc aatgtttgga tttgagatct
    25741 agttaattgc gtagaagtat ctgcgccatc tgcagatctt ctaatatctc cagcgctaga
    25801 caatagtcct tttattacaa caacatctgc tatttcagta tattcaggca ataccataaa
    25861 ttttcggaat gccagtactt ctaaacccat atatttagtg aaaataccat cgaatgtacc
    25921 gtcttcaaaa taaccattaa catataatcc atattgaatt attcttacat agtttcccga
    25981 gttaccttca gacacaggat aagttttact agttgtccca ttaccataag accctgttgc
    26041 ttcactggga gaatatccta gttcagcttg taatgcaaaa attaatgctt gatttgaatt
    26101 tcggttctgt tgcaaagttt taaatctact atcaaataag atagaataat agaaaaagat
    26161 agcaggagga ataacgatga atcattttaa aggaaagcaa tttcagcagg atgtgattat
    26221 tgtagccgtg ggctactatc ttcgttataa ccttagctat cgtgaagttc aagaaatctt
    26281 atatgatcgt g
//
LOCUS       DAZKCN010000032        25361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000032 DAZKCN010000000
VERSION     DAZKCN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.032"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..512)
                     /locus_tag="ACG8K3_001220"
     CDS             complement(135..512)
                     /locus_tag="ACG8K3_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145112.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /gene="purC"
                     /locus_tag="ACG8K3_001221"
     CDS             768..1496
                     /gene="purC"
                     /locus_tag="ACG8K3_001221"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9145113.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="ACG8K3_001222"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="ACG8K3_001222"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP9145114.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="ACG8K3_001223"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="ACG8K3_001223"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP9145115.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="ACG8K3_001224"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="ACG8K3_001224"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP9145116.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /gene="purF"
                     /locus_tag="ACG8K3_001225"
     CDS             4631..6070
                     /gene="purF"
                     /locus_tag="ACG8K3_001225"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9145117.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6093..7145
                     /gene="purM"
                     /locus_tag="ACG8K3_001226"
     CDS             6093..7145
                     /gene="purM"
                     /locus_tag="ACG8K3_001226"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9145118.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="ACG8K3_001227"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="ACG8K3_001227"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9145119.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="ACG8K3_001228"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="ACG8K3_001228"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9145120.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="ACG8K3_001229"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="ACG8K3_001229"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9145121.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="ACG8K3_001230"
     CDS             10664..11578
                     /locus_tag="ACG8K3_001230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9145122.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="ACG8K3_001231"
     CDS             complement(11711..13276)
                     /locus_tag="ACG8K3_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145123.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="ACG8K3_001232"
     CDS             complement(13269..14720)
                     /locus_tag="ACG8K3_001232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9145124.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="ACG8K3_001233"
     CDS             complement(14734..15750)
                     /locus_tag="ACG8K3_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9145125.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /gene="chvE"
                     /locus_tag="ACG8K3_001234"
     CDS             15970..17058
                     /gene="chvE"
                     /locus_tag="ACG8K3_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9145126.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /gene="mmsA"
                     /locus_tag="ACG8K3_001235"
     CDS             17112..18638
                     /gene="mmsA"
                     /locus_tag="ACG8K3_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9145127.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /gene="mmsB"
                     /locus_tag="ACG8K3_001236"
     CDS             18640..19845
                     /gene="mmsB"
                     /locus_tag="ACG8K3_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP9145128.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="ACG8K3_001237"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="ACG8K3_001237"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9145129.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="ACG8K3_001238"
     CDS             21343..22767
                     /locus_tag="ACG8K3_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9145130.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22809..23066)
                     /locus_tag="ACG8K3_001239"
     CDS             complement(22809..23066)
                     /locus_tag="ACG8K3_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145131.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="ACG8K3_001240"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="ACG8K3_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP9145132.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="ACG8K3_001241"
     CDS             24340..25317
                     /locus_tag="ACG8K3_001241"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP9145133.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8204 a         4317 c         5696 g         7144 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat t
//
LOCUS       DAZKCN010000033        24968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000033 DAZKCN010000000
VERSION     DAZKCN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.033"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(311..1093)
                     /locus_tag="ACG8K3_001242"
                     /pseudo
     CDS             complement(311..1093)
                     /locus_tag="ACG8K3_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1158..1994)
                     /locus_tag="ACG8K3_001243"
     CDS             complement(1158..1994)
                     /locus_tag="ACG8K3_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9145134.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2012..3421)
                     /locus_tag="ACG8K3_001244"
     CDS             complement(2012..3421)
                     /locus_tag="ACG8K3_001244"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9145135.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYRKVIARNGLA"
     gene            complement(3435..5258)
                     /locus_tag="ACG8K3_001245"
     CDS             complement(3435..5258)
                     /locus_tag="ACG8K3_001245"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9145136.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6101..6427)
                     /locus_tag="ACG8K3_001246"
     CDS             complement(6101..6427)
                     /locus_tag="ACG8K3_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145137.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6473..6835)
                     /locus_tag="ACG8K3_001247"
     CDS             complement(6473..6835)
                     /locus_tag="ACG8K3_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9145138.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6872..7087)
                     /locus_tag="ACG8K3_001248"
     CDS             complement(6872..7087)
                     /locus_tag="ACG8K3_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9145139.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7214..7549
                     /locus_tag="ACG8K3_001249"
     CDS             7214..7549
                     /locus_tag="ACG8K3_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9145140.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7625..9622)
                     /locus_tag="ACG8K3_001250"
     CDS             complement(7625..9622)
                     /locus_tag="ACG8K3_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145141.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9720..9995)
                     /locus_tag="ACG8K3_001251"
     CDS             complement(9720..9995)
                     /locus_tag="ACG8K3_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145142.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10227..10961
                     /locus_tag="ACG8K3_001252"
     CDS             10227..10961
                     /locus_tag="ACG8K3_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9145143.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11000..12142
                     /locus_tag="ACG8K3_001253"
     CDS             11000..12142
                     /locus_tag="ACG8K3_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9145144.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12237..13628)
                     /gene="sufB"
                     /locus_tag="ACG8K3_001254"
     CDS             complement(12237..13628)
                     /gene="sufB"
                     /locus_tag="ACG8K3_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9145145.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13633..14103)
                     /gene="sufU"
                     /locus_tag="ACG8K3_001255"
     CDS             complement(13633..14103)
                     /gene="sufU"
                     /locus_tag="ACG8K3_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP9145146.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14090..15325)
                     /locus_tag="ACG8K3_001256"
     CDS             complement(14090..15325)
                     /locus_tag="ACG8K3_001256"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9145147.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15322..16608)
                     /gene="sufD"
                     /locus_tag="ACG8K3_001257"
     CDS             complement(15322..16608)
                     /gene="sufD"
                     /locus_tag="ACG8K3_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9145148.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16621..17394)
                     /gene="sufC"
                     /locus_tag="ACG8K3_001258"
     CDS             complement(16621..17394)
                     /gene="sufC"
                     /locus_tag="ACG8K3_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9145149.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17591..18439)
                     /locus_tag="ACG8K3_001259"
     CDS             complement(17591..18439)
                     /locus_tag="ACG8K3_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9145150.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18463..19149)
                     /locus_tag="ACG8K3_001260"
     CDS             complement(18463..19149)
                     /locus_tag="ACG8K3_001260"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP9145151.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19142..20176)
                     /locus_tag="ACG8K3_001261"
     CDS             complement(19142..20176)
                     /locus_tag="ACG8K3_001261"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP9145152.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20239..20495)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20571..20909)
                     /locus_tag="ACG8K3_001262"
     CDS             complement(20571..20909)
                     /locus_tag="ACG8K3_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP9145153.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20911..21267)
                     /locus_tag="ACG8K3_001263"
     CDS             complement(20911..21267)
                     /locus_tag="ACG8K3_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP9145154.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21273..22460)
                     /locus_tag="ACG8K3_001264"
     CDS             complement(21273..22460)
                     /locus_tag="ACG8K3_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9145155.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22651..23496)
                     /locus_tag="ACG8K3_001265"
     CDS             complement(22651..23496)
                     /locus_tag="ACG8K3_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9145156.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23730..24695
                     /locus_tag="ACG8K3_001266"
     CDS             23730..24695
                     /locus_tag="ACG8K3_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP9145157.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7309 a         5251 c         4244 g         8164 t
ORIGIN      
        1 ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa cagtactgtc
       61 tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac tacctgaaac
      121 agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat ttcagataca
      181 aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt atcctaagct
      241 actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa tgaaaaagat
      301 ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt acctgtccgt
      361 tcttaaatac cacagaataa tctttttacc cattttcaag taaccgaagt ggaatgtctt
      421 taccaacttg ctctgtcact gcattactga tacctgttag attagctaat aaatctgata
      481 aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct ttatcttcat
      541 ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt
      601 catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa tcgattcttt
      661 catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa actctcttca
      721 cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc acgcttttac
      781 taataaattc actataacca gttctttcaa tagagacttc aatgtatttc acactatcat
      841 actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca taataatcct
      901 ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat atctctatac
      961 cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag ctagtagttt
     1021 cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc gcactgttag
     1081 aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat atattgtgca
     1141 gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata aagcagttcg
     1201 tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt caaggccgcc
     1261 cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc cttctccggc
     1321 aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac aaatcttaag
     1381 taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag catagccaag
     1441 atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg ttcactttgg
     1501 gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg aacattcatt
     1561 tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg gtaatacttt
     1621 tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg ttcaatggca
     1681 tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg
     1741 gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg agcagcctca
     1801 aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt aaatactttt
     1861 tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat ccctttcccc
     1921 atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt
     1981 ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt gcgattactt
     2041 ttcgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga ttctcatcgg
     2101 ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta ctaacaacat
     2161 caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa gctgcttgaa
     2221 tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt cgtctatctt
     2281 cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt aaatcatagc
     2341 gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc cagccatatg
     2401 gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct cccaacatga
     2461 tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg gcagcgatgg
     2521 tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat aaaattgcct
     2581 gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt tcaaacaaat
     2641 aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat gtttcccaat
     2701 tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc gttaaattga
     2761 ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt actctagctt
     2821 gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc agctctccac
     2881 ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca ttaattgtca
     2941 gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag tccacaaaat
     3001 aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga
     3061 aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca attacttgat
     3121 gataaaaaga aattcccttt tgattggttt tattattttc aatatccata attcgcgtcc
     3181 aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt atatcttctt
     3241 tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct gtatgctgat
     3301 acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct gcctggtagg
     3361 cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca
     3421 caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa gcttccgctt
     3481 gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac tgtatcatcg
     3541 tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc tgctgacacc
     3601 tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc
     3661 cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc atgtttagat
     3721 tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc ttctggatga
     3781 atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac ttcagaaata
     3841 ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat tgccgtttgc
     3901 tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa ggttgcaaca
     3961 aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc tccaataaag
     4021 gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc gctcaagcca
     4081 gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt tttcagcttc
     4141 aaggttactc catagagtga aggctcagta attcccatta aagcagtgat cccagtggac
     4201 acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa ggtagcacct
     4261 gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga acccgtactg
     4321 ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac caccaaaaat
     4381 ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt aatccattca
     4441 gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac aaccaacgct
     4501 aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat aaacgaaggc
     4561 gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa gataatcggt
     4621 aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga aacggcatcg
     4681 ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc
     4741 aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac tggcataaaa
     4801 taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact gtctgatagt
     4861 acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc tgttatagct
     4921 ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat aatactttct
     4981 tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg aatttccttg
     5041 aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct atccaccaca
     5101 ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt atctttgagt
     5161 tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt tcccccaacc
     5221 tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc ttaaagcagt
     5281 aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt gtcttggtct
     5341 tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa taatagcaca
     5401 ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga ccgtataggg
     5461 taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac tgctaaaata
     5521 agtatatcta atattaacta taccagtaaa accattccta cataaccagc gggaaattcc
     5581 caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa caattctgct
     5641 cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat tccaacaata
     5701 taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata gccaatttca
     5761 atcattccga gatataaagg tatcaataga atgatttggg ctagtattct taaaaattaa
     5821 ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt tcaattccac
     5881 caattatcaa taaaaatacc aaacatatcc ataataattt tttctttttc atttctttta
     5941 tattgttata acagtgaata acataaaaat tcccgacaat cattgtaata tttttttgtc
     6001 acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa tttacacctt
     6061 tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac attaacacct
     6121 cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca atatttatgt
     6181 tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct ccattttcat
     6241 gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt aaaatctgtg
     6301 ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt gaaaaaataa
     6361 tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc atatttactt
     6421 tattcaactt tttatctcct atctccaaaa taatttgttc ttatccctct tatcagtaca
     6481 ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc atcaaaatcg
     6541 tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca acagcaccaa
     6601 atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata atcaaataca
     6661 gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt gtctgctcag
     6721 tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt gctccacaaa
     6781 actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa tacatgtctt
     6841 ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta aatacatcag
     6901 aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt tgatagggtt
     6961 taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca ttcatttttt
     7021 ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga ttacttactt
     7081 ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat ttcaactcat
     7141 aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac tatactgaga
     7201 tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa agcgaaaagc
     7261 tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac aggcagtagc
     7321 aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc tttctagagc
     7381 cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg aggaagagtt
     7441 tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg actatatgaa
     7501 gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa aaataaataa
     7561 cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc tatggataac
     7621 ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca tcatctcttg
     7681 ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc aattctttta
     7741 ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta cttgctttca
     7801 cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct tgtttaacaa
     7861 agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca ttacgtaggc
     7921 gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct tgccacgata
     7981 agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg ctgagttctg
     8041 atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat atcttaaccc
     8101 attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga aggatatctt
     8161 cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag acattgcttc
     8221 tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg tattcgagca
     8281 tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa gtcataagtg
     8341 atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa ctcatttttt
     8401 tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca tcaaaaaaat
     8461 taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa ttccttattg
     8521 tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt gattcctctt
     8581 tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa ttaaatgttt
     8641 ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt tcactgatat
     8701 cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta aaaatactag
     8761 cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac ttaagtaatt
     8821 tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg tcggaggaat
     8881 caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt tttatcaact
     8941 taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt tgacaatttt
     9001 tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg ttgtcttcat
     9061 taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc gaagcattta
     9121 cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca accattttac
     9181 catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct agattttcag
     9241 agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg atagataaaa
     9301 ccttttctat gcttaagtat ctactatcat ctttactcaa atctactacc ttccccttct
     9361 catctttaac agtgttatcg acatccagaa taattactct ggtttccaca aaactctcct
     9421 tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa tttagtatct
     9481 ctacaaactc atctcttgta acttctctat agttatctaa acacctatct cttacatcag
     9541 ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca taaattggaa
     9601 tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt aaagactaaa
     9661 acatacagat aataaaaaca aactctttat tactatatgt tttagtctta tttgtttact
     9721 caacctttat accgttccat gaaactatca atatcagatt ttttaaatct aatgaccgta
     9781 tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt tcctactgat
     9841 acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac tccagaaata
     9901 atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg ttcaacttgt
     9961 atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt cgtgattgct
    10021 tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag tattttgtta
    10081 ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca taatacctcg
    10141 ataaaaaagg ggtgtcaaca gctttgtaaa atttatttac aacacccctt ttttgaggta
    10201 tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat tatttagaac
    10261 gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac
    10321 cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact atctacgaat
    10381 gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct gatttgggaa
    10441 atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg ggaattattc
    10501 tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac ctgttccagc
    10561 atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg gatagatatt
    10621 ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat aaagttaaaa
    10681 atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc aatgctattg
    10741 tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa ctactagaaa
    10801 gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc gaagaagctg
    10861 tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca attgggaaaa
    10921 aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta ctccttgtaa
    10981 actaagagga gatgatttaa tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt
    11041 ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa aaataaatac
    11101 tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg agtacagaca
    11161 tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag acctgtataa
    11221 tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa tctcagtaag
    11281 atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga atatctctgt
    11341 cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt acgattatat
    11401 gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata ttgacaataa
    11461 tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta
    11521 ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta acatgaaaca
    11581 atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag tactcgcttt
    11641 acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa ctcttgctgt
    11701 agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta gacgagttat
    11761 ctctttagat actgagactc tttctatcct caacaactgg aaattacagc aaaaagaaga
    11821 gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta caacagttaa
    11881 aaacactcta tacattccaa acactgttaa tgattggtta agatatattt tgaaaaaata
    11941 taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt tattattaga
    12001 agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata gtaaagtgac
    12061 attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc aagactttgc
    12121 tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct agtatacagg
    12181 caaaacaaaa aagctagaac ccttatatat caagggttct agctcctata acaggtttac
    12241 ccaacactgc cttccatctc ataactgatc aatcgattga gttcgactgc atattccatt
    12301 ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt tgcttcagat
    12361 tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt tgagactttg
    12421 gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat cgtatcggat
    12481 ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga acccgcactg
    12541 tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt tgaaattgat
    12601 ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc acccgtatct
    12661 tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc accttttcca
    12721 tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat
    12781 tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta cacattgtct
    12841 gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg
    12901 atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc gacatagtgg
    12961 atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt gttttccgca
    13021 ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg cacgtaaata
    13081 aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc ggttggcggt
    13141 acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc agagtcagtg
    13201 tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta cacgacttct
    13261 gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg tataccaatc
    13321 ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc gggtttgtca
    13381 cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat
    13441 gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc aagcatccat
    13501 tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa gccttctcct
    13561 gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc taattctggt
    13621 acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt
    13681 ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc gagcgggaaa
    13741 tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt tgacttcttt
    13801 tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta ttgattttcc
    13861 gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc cactgccgtc
    13921 aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga tcacatctcc
    13981 acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg
    14041 cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt tagatagtcc
    14101 catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg
    14161 ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg tacttcaata
    14221 gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct aaagctgtcg
    14281 cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg
    14341 ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc aacacataag
    14401 cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc aaataatcga
    14461 ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg aatttccaag
    14521 gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt
    14581 caacaggttc catttcttct aaccattttc gtttaccata taaaacacca atacctgttg
    14641 gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa
    14701 tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg ttttcatgga
    14761 caagttcagc taattcttga acaggattga agacacctaa gacattggat acatgagcaa
    14821 tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata tccaggaagc
    14881 cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc aactgctgcc
    14941 acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct cctgctttga
    15001 tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa
    15061 acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct cttgcttgtt
    15121 cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca ttggcgttat
    15181 cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg
    15241 cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata ggaaaatcat
    15301 tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt cgactaattc
    15361 tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc ctcgaatcac
    15421 taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca tttcttctgg
    15481 atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat cgatcaaaag
    15541 aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac tttcttgttg
    15601 ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg tcaatgttcc
    15661 tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg ctttattggt
    15721 tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga ttttgacttc
    15781 tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt
    15841 catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac ggcgattcat
    15901 ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt ttgaaccagc
    15961 tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt ctttggttgt
    16021 ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca acacatgctt
    16081 gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg gttcatcgat
    16141 cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta catggagtgc
    16201 agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt ggactagttc
    16261 tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt ctgctaattc
    16321 cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag gattgtcttt
    16381 cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc
    16441 aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca gttcatctgc
    16501 tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt tacttaaaga
    16561 aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc ttgctccccc
    16621 ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag cgtagccttc
    16681 tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc cttccatcat
    16741 gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg tgatgatcaa
    16801 tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt tcaaagcatc
    16861 gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta acatcaacaa
    16921 ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca gataacgttc
    16981 agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt tcaaaaatgc
    17041 cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac gcatgaattc
    17101 cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata agccaagtcg
    17161 cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa tttcgccttc
    17221 tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt ttcctgtgcc
    17281 gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta cgcctttaag
    17341 gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag acatgcatta
    17401 tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg aagcgcaatg
    17461 accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg atttctaaat
    17521 tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct catctttttt
    17581 gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc catttgtctg
    17641 tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg attttgtcac
    17701 tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg tccttttcaa
    17761 gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt gcgttgattg
    17821 caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg tcgatcgtat
    17881 ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct gtttccagat
    17941 ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg atgcgtcccc
    18001 agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg gaaacatctt
    18061 tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca ttgacaaagt
    18121 catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag taattggcat
    18181 caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat ttgacgattt
    18241 caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg
    18301 gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc
    18361 tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct gctgctaatc
    18421 caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt tgtctgtttt
    18481 cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa taattaaagt
    18541 cgcaacgaat gtcactgtca gattaccacg ctggtagcct tcctgccaag caagacctcc
    18601 aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg agatcgttgt
    18661 aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct tccagatgat
    18721 ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc tgacttcacg
    18781 aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa gagctggaat
    18841 ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa tgatcaaaac
    18901 catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata cgattccata
    18961 aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca atcccaaaac
    19021 taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg tatcaaggat
    19081 agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt ctgaaaagct
    19141 tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac acttctgctt
    19201 gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag gaaccttctt
    19261 tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt cgaatcgctt
    19321 gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc aaacggccgc
    19381 ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct ggttcgatgt
    19441 cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca gtcaatacgt
    19501 ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag attttgcgga
    19561 tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta ttgatcgcta
    19621 acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta gcctcatcac
    19681 aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt tgtttctgcc
    19741 cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact aaacgcaaca
    19801 gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc agcggcagct
    19861 ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag atcataccga
    19921 ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct tgatcgttta
    19981 cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact aatgtacttt
    20041 ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg tttagtgaaa
    20101 cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg acattcgtta
    20161 gttggattaa tgacatgcca ttcacatctt tctcttatga tttttcaaac aaaaaagcct
    20221 tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac caccttgatt
    20281 taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct ttccgtatac
    20341 tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta ctctgctcta
    20401 agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc tcttcaaacg
    20461 tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt gcttatgatt
    20521 atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt ttataattcg
    20581 tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat ttcatctgaa
    20641 tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt
    20701 agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag aggttcatct
    20761 ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc aagttcttct
    20821 tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat ccaaaggttg
    20881 tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat aaattttttc
    20941 attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggccgct tgatcagcat
    21001 accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta cttgatcttt
    21061 cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga ttggtacttg
    21121 actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga tcatatcaac
    21181 agtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc atgttgaaca
    21241 tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct tgcaggtgct
    21301 tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc taaaatcgaa
    21361 gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa tccgatattt
    21421 gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct tgcgatatag
    21481 gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat taaaataaaa
    21541 tacaagaaaa tgacaaatgc accaccgata aagccgaagt tttcaccgac aacggtaaaa
    21601 atcatatcgg actcacgaac aggcacataa atattgctta cgttgaatcc tttaccaaac
    21661 aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat tcctgacgta
    21721 tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt gaagccaact
    21781 ttgtaaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc tgctccaacg
    21841 actactgcca agattactac aggtgcgatg atgcgccaag agattccaga catcaagaaa
    21901 atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg ggccatgatc
    21961 aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa gtccgatttc
    22021 atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat catcaagatg
    22081 tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg aaaccagttt
    22141 ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag tcccattacc
    22201 actaacccca aaccataaaa gtaaggagtc agtttccaga tccatttgga attcaaatgc
    22261 atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat ccctacgcct
    22321 ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga gagcagacca
    22381 atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt atcgttcgaa
    22441 gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg ctgattatta
    22501 tagcggtcca attagataat ggaaagtctt tccatagaca tttgggaaaa attaactttc
    22561 cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc tttagtgtat
    22621 catagaactt tttttgatac atgcaattat ctaccaactg agattctcct cgctgtgaga
    22681 agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca gctgatcaga
    22741 aaccagattg gttcgttgga tgaaataaaa ataacgatgg aaattcaacg gagtcattgg
    22801 cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt ccttcagcaa
    22861 cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat tcatctcttc
    22921 aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa gccacaattt
    22981 gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc tttgtacgcc
    23041 ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg ctacttgttc
    23101 taatgattca tgagaattcg cgatttcgat ctcaaaatgc actttaggaa accttttgat
    23161 cagatgtttc atcaactgag gcaaaggata gacagcaaag gtgtgggaag caacgattcg
    23221 acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt cccattcttc
    23281 taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa gcatctcttt
    23341 tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt tgatttgatt
    23401 cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc
    23461 atatactgcc cgaaaagtcg ttaataactt aaacatggca ccacttccat aactttttat
    23521 gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat aatagaaatg
    23581 tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca gctgtctgct
    23641 tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt ggtactaaaa
    23701 tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt attccgatcc
    23761 cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa gactatcacc
    23821 atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata ttcatttttg
    23881 ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca atcatcgcct
    23941 ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt gtttcatatg
    24001 aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc caagtgatcc
    24061 ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt gaagcaattt
    24121 atccaagctg gtttatcatt tatgttggat ttggggtcat cactgtaact gccgggaact
    24181 tcttcccgct tattggaaaa attttctttt ggatttcatt gatctgctat gcgttcttat
    24241 tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg acacgtccgc
    24301 tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt aaaaactttg
    24361 ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg
    24421 ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc tatgcagcct
    24481 ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat tacttcaaca
    24541 gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa
    24601 gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa gaacatagca
    24661 aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa
    24721 agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca tgtttttgaa
    24781 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
    24841 aagaagtggc cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt
    24901 gtgaaatttc ggcttatttc cgaaaaagct acttctggaa caccgtttac caagaggtcg
    24961 tgaaaaag
//
LOCUS       DAZKCN010000034        24780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000034 DAZKCN010000000
VERSION     DAZKCN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.034"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1950
                     /locus_tag="ACG8K3_001267"
     CDS             52..1950
                     /locus_tag="ACG8K3_001267"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP9145158.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2249..2968)
                     /locus_tag="ACG8K3_001268"
     CDS             complement(2249..2968)
                     /locus_tag="ACG8K3_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP9145159.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3158..3205
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3305..3637
                     /locus_tag="ACG8K3_001269"
     CDS             3305..3637
                     /locus_tag="ACG8K3_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145160.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3624..5627
                     /locus_tag="ACG8K3_001270"
     CDS             3624..5627
                     /locus_tag="ACG8K3_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP9145161.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5646..6116
                     /locus_tag="ACG8K3_001271"
     CDS             5646..6116
                     /locus_tag="ACG8K3_001271"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP9145162.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6137..6721
                     /locus_tag="ACG8K3_001272"
     CDS             6137..6721
                     /locus_tag="ACG8K3_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFP9145163.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6736..7722
                     /locus_tag="ACG8K3_001273"
     CDS             6736..7722
                     /locus_tag="ACG8K3_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP9145164.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7712..8023
                     /locus_tag="ACG8K3_001274"
     CDS             7712..8023
                     /locus_tag="ACG8K3_001274"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP9145165.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8045..9826
                     /locus_tag="ACG8K3_001275"
     CDS             8045..9826
                     /locus_tag="ACG8K3_001275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP9145166.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9819..11195
                     /locus_tag="ACG8K3_001276"
     CDS             9819..11195
                     /locus_tag="ACG8K3_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP9145167.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11202..11837
                     /locus_tag="ACG8K3_001277"
     CDS             11202..11837
                     /locus_tag="ACG8K3_001277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP9145168.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11867..13222
                     /locus_tag="ACG8K3_001278"
     CDS             11867..13222
                     /locus_tag="ACG8K3_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP9145169.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13414..14808)
                     /locus_tag="ACG8K3_001279"
     CDS             complement(13414..14808)
                     /locus_tag="ACG8K3_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9145170.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14900..15871
                     /locus_tag="ACG8K3_001280"
     CDS             14900..15871
                     /locus_tag="ACG8K3_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP9145171.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15868..16649
                     /locus_tag="ACG8K3_001281"
                     /pseudo
     CDS             15868..16649
                     /locus_tag="ACG8K3_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(16818..17189)
                     /locus_tag="ACG8K3_001282"
     CDS             complement(16818..17189)
                     /locus_tag="ACG8K3_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9145172.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            17323..17766
                     /locus_tag="ACG8K3_001283"
     CDS             17323..17766
                     /locus_tag="ACG8K3_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP9145173.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17809..18618)
                     /gene="yidA"
                     /locus_tag="ACG8K3_001284"
     CDS             complement(17809..18618)
                     /gene="yidA"
                     /locus_tag="ACG8K3_001284"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9145174.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18637..20004)
                     /locus_tag="ACG8K3_001285"
     CDS             complement(18637..20004)
                     /locus_tag="ACG8K3_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9145175.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19988..20818)
                     /locus_tag="ACG8K3_001286"
     CDS             complement(19988..20818)
                     /locus_tag="ACG8K3_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP9145176.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20981..21418
                     /locus_tag="ACG8K3_001287"
     CDS             20981..21418
                     /locus_tag="ACG8K3_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP9145177.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21494..22114
                     /gene="rpoE"
                     /locus_tag="ACG8K3_001288"
     CDS             21494..22114
                     /gene="rpoE"
                     /locus_tag="ACG8K3_001288"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP9145178.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22303..22809
                     /locus_tag="ACG8K3_001289"
     CDS             22303..22809
                     /locus_tag="ACG8K3_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP9145179.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22826..23725
                     /locus_tag="ACG8K3_001290"
     CDS             22826..23725
                     /locus_tag="ACG8K3_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP9145180.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23812..24697
                     /locus_tag="ACG8K3_001291"
                     /pseudo
     CDS             23812..24697
                     /locus_tag="ACG8K3_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT         8065 a         4332 c         5289 g         7094 t
ORIGIN      
        1 gagcataaca actttataat gttgagtata ccaatttttg gaggcgtttt aatggaccgc
       61 tatttagatt tactttcaga gaagttccct acaacggaag atgtcatcac ggaaatcatc
      121 aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag cgatatacat
      181 ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag
      241 atcaaagacc ttttctctga acaaatgacg gaaacggaga tcagccaatt cactattttt
      301 acggcttatc cagaatatgt gttgacgact gattggtaca aaaaacaaga taaatcccag
      361 ctgatccatc gtttgatcga tcttttacgg tttacgacag tcaaatacac tcgatcaaaa
      421 gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat
      481 gatcgtatca acgggaaaaa agactatgta aaaaaaatca ttgaacaact tgtcttgatg
      541 aaggaagaag aaaaattttt aacaaaactt gcgcagacga tccaaaaatc tgtcatcgat
      601 catttgcata tcgtgggtga tatttttgat cgaggaactc aagcagccaa agtaatggat
      661 cgaatcatgg atttttcctc tgtagatatc cagtggggga atcatgatat tttgtggatt
      721 ggtgcgtatt gcggctctga ggcatgttta ttgacgcttt tacggattgc cgcacgctac
      781 aactatttat ttgaattaga gaaagaatat ggattaaatt tacgtccatt attttctttt
      841 gcccatcaaa cctatcagaa gaatccagta tttaccccta aaaacaggaa agatctatct
      901 gaaagagagc aagaagaatt agaaaagatc catcaagcac tttctatcct ccaattcaaa
      961 tcagaacatc agttgttgaa tcgacgacct gaatttaaaa tggacgatcg taaattgttg
     1021 gaaaaaatca actatgagga atcaacgatc gttttagaag gtcagctcta tgagttgaaa
     1081 gggacttgtt ttcaaacgat ccagccagat gatcctaaaa aattcttgga agaggaacaa
     1141 aaagtgatcg atagtttgat gtattcattc cagcactcgt taagaatgca aaaacacatg
     1201 acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt actgttccat
     1261 ggctgtatac ctgtagatga aaaggggcag ctcttagcat ttgaattaga ccaatcttat
     1321 cgcggaaaag aactgctggg attttttgaa aaacagataa ctgaaagcgc aaaagatttg
     1381 actgcaaatg aagatttggc gaccgatttg atttggtatg cgtggagcgg gccattttcg
     1441 cctttattcg gcaagagtaa aatggcaact tttgagcgct attttctcac agagcctcat
     1501 acgcacgtcg aaaaaagcaa tccctattat cacttgcgag atgaagagtg gttcagtgaa
     1561 aaaattttgg cagaattcga tgcaaaagaa gaaggttcag caatcatcaa tggacacacg
     1621 ccagtaaaag taaaaaaagg agagtcaccg atcaaagcaa acggaaaagc atttgttatt
     1681 gacggaggtc tatccaaagc ttatcaaaaa acaaccggga ttgctggcta ctcattacta
     1741 tgcaattctt acggtttcca aatcgtgaca cattatccgt tcttgcctat tgaacaacaa
     1801 tttgcaaaaa aatctgatca aacgaatgta aaaaaagtga tcgagcagca gctcccacgc
     1861 aaacttaatc ggaatacgac aaaaggaatg gaattacagc aacagattac tcaactccaa
     1921 aggctgttgg attatcgaaa ggacgattga gaaaaatgct agccaacatt gctcaataga
     1981 ctaaaaagcg actgcaacaa aaggcgtgtc gcagtctcct tcggaattag ctcaaaaaac
     2041 ggtaaaatat tagaagctat ttttctgttt atttctctaa tactcaggtc agggcgctga
     2101 tttcacgacc tcttccttaa acggtgttct cctgccaact cctcggtcac aagtcacaat
     2161 atccaacaaa catgagaagc tgttttttga aattgctcct tgtgactcag agttgaacgg
     2221 caggctcacg acctaatcag aaaaacgatt aaaattcatt cacaaagaaa tcgaataaac
     2281 gctgttcaga tgaatctaca cgatgagaaa gttccggatg ccattggaca ccaaggattt
     2341 tgtgcatatt atctttactt tggactgctt caatgagtcc atcgttggaa aaggcgatgg
     2401 cttttaacaa aggagaaaga tctttgactg cctgatgatg ataggaattc acaaagtatg
     2461 aatcagggag aaggtcactt aataaactgt ccgaaacgat tttgacttcg tgagttgcaa
     2521 actgcggggc agtgggctgt tgttcgtgtt ttacggacca ttttggatat aatgaaagat
     2581 cttgataaag tgttccctcc aacacgacat tcaatagttg aagtcctcgg cagactgcaa
     2641 aaatcggttt tctttgtttc aatgcttcca agatcaatgc ctgttcgaat tggtctcgtt
     2701 gaatatttgt ttccgtcagt tttggatgag ggtcttccat gtaaagttgc ggactaacat
     2761 cctgaccgcc tgcaagaatg agtttgtcga ttttctcaat tagttgaggc gctgaatctg
     2821 gagacatgac tggcagaata agaggtaatc cttgagcatc taaaactgca tccacaaatc
     2881 cctgcggtgt atagcttact tgatttcctt ggaaagtatc tgtcgctcga atcagttgat
     2941 tcccggcaat gccgatgatt ggtttcattt ttattccccc taaagtagac taattgtaac
     3001 aaggatttaa aaaaatttaa actatgtaag acttgcccct atacttatct gttcttgctg
     3061 tgttactatc tgtaaagaga attttcgtgt gaaattcatg acgatagttg aaaatattgc
     3121 ttaatttgtt ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat
     3181 ctagttcttc aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat
     3241 ctttagaggt acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa
     3301 ggaagtgaag caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga
     3361 gaagcgtgct cgaaccctac ggcagcagtt gaagacctat gcagaagaaa aagagcaaga
     3421 attggagata atccaaacga acagtcggca acagctgaaa cagttggtag aagaaaaaga
     3481 acaattcgaa aagcaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa
     3541 ggtgcaagca actttgaaag accaatatca aaagcatcag agtcaagcaa tcggtgcaat
     3601 cattgaaagg gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt
     3661 cagataaaag aaatcaggag aggattttac aagcagtaca aggaatccaa aatgttgaaa
     3721 tccgggatct gtatcaaaat agaacaaaca accagtgggt agaaaaatat tttccagata
     3781 ccacgattat tgataaagaa gcaaaattaa gcgctttagg caatcggctg aaagacatcc
     3841 aagaagctat ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga
     3901 aacgtcaaga actttcctta caagacttgg aagcaaatta ttccgaagaa aatttcatga
     3961 ataaattaga agaggtcctt gatttaaaag aaaagtggga acagctaact gaaaaacgtg
     4021 atcgtttaga agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa
     4081 gtatgtatgc aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg
     4141 aaagtttcaa ggaagaacta agtaagcttg atgacgttta tatggaagaa atagattata
     4201 cgccaaaaca agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gctatagaag
     4261 aaattgctgg gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag
     4321 aaaaattgca agaaaccaaa cagcaattat ccattgttca cgagcagcaa aaaaagataa
     4381 cgacagcgtt aggtcagtgc agcggctata tccaagaatt tgaatgggta gaagaagtca
     4441 cacttgccgt tgcagaacgg gaaaaagtaa aagaacggtt tatccatgcc acttatctga
     4501 tcgttttgca aggttgggta gatgcagaag aaaaacagaa tttgatgcat gtgttaaacg
     4561 aaactgtatc agaagaagat atatacgtta gtttcgaaac accaaactca gaagagattc
     4621 agcaggaagt ccctactaaa ttgaaaaacc atccgcttat cgaacctttt gaattgctga
     4681 ctgaaatgta cagtctgcca aaatatgaag aagtagatcc aacgccttgg atgacaccat
     4741 tttatcttgt cttcttcggg atgatggtcg cagatgttgg ttacggattg ctgatgctca
     4801 ttgggacaat actagctcaa aaactatttg ttttaccccg cgggatgaaa cgtttcgtaa
     4861 aattctttga gattctctcg gtaccatcga tcatctgggg attgatctac agttcatttt
     4921 tcggacaggc attaccaaaa gaactatttg gtatccgctt gcctttccca atcctatcta
     4981 cgacggatga cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagatct
     5041 tggttggatt attcgtggca gcaaaggaaa atatcaggag gaaagattac ttgagtgctg
     5101 tcagtgatgg ttttgcctgg caagggatcc tgattgggat cgttattgca ttgattggtg
     5161 tcatgttgat aaaaaataca gcatttgttt atcttggcgc ggggctatcg atcattgctg
     5221 ctttatgtat cttaatcgtg ccgattatcc aatcgccgtc taaagtcaag ggtgctgcca
     5281 aaggagcgta taatctttac ggattgactg gttatatcgg tgatttagtc agttatacac
     5341 gtttgatggc actaggtatt tcaggcggaa gtattggcgc ggcattcaat atgctagttg
     5401 ctttcatgcc gccagccgct cgattcagtg tagggatcct tttgatcatc gctttacacg
     5461 cactgaatat gtttttgaca ctgttaagtg cctatgtcca tggtgcacga ttgcagtatg
     5521 tcgaattttt tggaaaattt tatacaggag gcggacgtgc ctttgacccg ctgaaaacgg
     5581 cggaaaaata cgtcaacatc aatcataaga aaaaaaacaa taaataattg gaggattttt
     5641 atacaatgat ggattactta attagtcaaa atggtggaat ggtatttgca gtattagcga
     5701 tggcaacagc gacgattttt tcagggattg gttcagctaa aggtgttgga atgacaggtg
     5761 aggcagcagc agcgttaaca acaagtcaac ccgaaaaatt cggacaggct ttgattctgc
     5821 aattgcttcc aggtacgcaa gggttgtatg gattcgttat cgcctttttg atttttatca
     5881 acttaggaaa tgatatgtca gttgtacaag gattgaactt tctaggcgct tctctgccga
     5941 ttgcttttac aggtttgttt tcaggtatcg cccaagggaa agttgcagca gcaggtatcc
     6001 aaattttagc gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg
     6061 aaacatatgc gattttaggt ttcgttattt ccttcctatt agtattgaat gcctaagcat
     6121 aaaggaggaa aagcagatga acgccattga aaaaatcatc tctcagatga atgaagcagc
     6181 agagcaagaa agagcggcgt tagagcaaga agagcgaatg aagatcgatc aaaactttga
     6241 acaaaaacgt acgcaaatcg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt
     6301 acttgaaaaa aaataccgtc agcttaggaa tcgccaacaa gtagaagtac gacaagaaaa
     6361 tttgaatgcg aagcaggaat ttttacgacg attgtttgca gatgcaatag ctgaaatgga
     6421 gaattgggat gaatctgaac aaatccagtt tatcaaaaat tcattgtatt cattaccttt
     6481 gacaggcaaa gtaacattca ttgctggaga aaaatcagca gcttatcttt ctcagacatt
     6541 gctggatgaa tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc
     6601 tgatcaagcg ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag
     6661 ccttgtacaa gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata
     6721 aggagggatc agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt
     6781 gaacttttgt ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt
     6841 ggtgaaatat tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac
     6901 ttcgaacaga atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca
     6961 ccggagcctg aaatcgtttg gatctatacg atgcgctata ctttccataa tctgaaggtt
     7021 ttgacaaaag ccgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt
     7081 tattcattag aaaccttgaa ggatgccatt catacgcaag tttcaaatga actccctgaa
     7141 agcattatgg acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag
     7201 ggaattgacg tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag
     7261 ctaggatatc aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca
     7321 acgatggctc gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc
     7381 agctcgggaa gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat
     7441 tacacgaatt ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag
     7501 ggaaatcttg atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat
     7561 caagaagcac aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa
     7621 gaggtagaaa gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaacca
     7681 gaaacaatca gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga
     7741 caaagattct gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa
     7801 gcaaaccgtt cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt
     7861 cacagagcaa tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat
     7921 acctgctatc gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat
     7981 tcaaaaaaac gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga
     8041 aattttgcaa attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat
     8101 gtctgatgcc agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat
     8161 tatcgagatg cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg
     8221 tccaggcgaa ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat
     8281 catttcacaa atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca
     8341 aagtaatttc ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg
     8401 gttcgaacca acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt
     8461 agaagaaaca aaagttataa aacataaaat catggtgcca aatgggataa aaggtacgat
     8521 caaaacaatc gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga
     8581 aaatggtgac aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat
     8641 caaagaaaaa ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt
     8701 cttcccagtg acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac
     8761 agtcgttcag catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg
     8821 tggcgagcgt ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc
     8881 taatacagga gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc
     8941 agtagcagct cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga
     9001 catgggttat gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg
     9061 tgagatgagc ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg
     9121 ttcacggctt gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg
     9181 tcgcgaagga agtataaccg caatcagtgc tgtatcgccg tctggagggg atatttcgga
     9241 accagtcaca caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt
     9301 agcccaaaaa agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac
     9361 agaagtaggg aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga
     9421 gggcatgcgt attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat
     9481 tgattccctt tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga
     9541 ttatttgcag caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt
     9601 caaaatgttg aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg
     9661 tgcttattta agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag
     9721 caaatatatc ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca
     9781 aacgatgcaa acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga
     9841 tcggtgaagt cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag
     9901 aattaatcga agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc
     9961 aagaagataa agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact
    10021 cttctgtacg atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc
    10081 gagtattcga tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc
    10141 atttggatat taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta
    10201 tccaaacagg tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac
    10261 cagtattttc tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag
    10321 caacggtact cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt
    10381 ttgaagaagc agaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg
    10441 tcatgtttat gaatttagca aacgaccctg caatcgagcg tatcgctact cctcgtatgg
    10501 ctttgactgc tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga
    10561 cagacatgac caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc
    10621 caggaagaag aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag
    10681 caggaagaat ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag
    10741 aagacgataa aactcatcca atccctgact tgactggtta tattacagaa ggacagatca
    10801 tcttgactag agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc
    10861 tttcgcggtt aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta
    10921 cgatgaatca gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttggccgtag
    10981 ttttaggtga atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt
    11041 ttgaaaaaga atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg
    11101 atctagggtg ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg
    11161 acatgctgga caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga
    11221 atccgacccg catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc
    11281 ataaattatt aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa
    11341 agaacaacca gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg
    11401 tattggcaaa tgcttcgatc acggagcctt tcatcgaaga attatttgtt ttgccggcgg
    11461 aaaacgttga tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga
    11521 attttgaata tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca
    11581 gtgccgaact ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac
    11641 tgacagaaat cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc
    11701 gagtcaatgc attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc
    11761 gtatgaagct ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata
    11821 tgggtaccac tgactaagtg gtatcctttt ttaaaagagg aaaacgatga ttaaacaaag
    11881 caaacaaata aagaaacgct tgccggctgt tcagcttctc gcattaggat tttttctcct
    11941 gattttagtt ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac
    12001 aaattttatc gatgcgcttt tcactgcaac ttctgctgtc tgtgtgacgg gattgacaac
    12061 attgaataca gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga
    12121 gatcggcggt cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa
    12181 agtcagtctc agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg
    12241 tgtgatgaag ctcatgcttt acatcctgaa attggccttt gtgatccaac tgattggtac
    12301 ggttgcttta tcttttgtat tcgtgcctga atttggctgg ttgaaaggta tctggtacag
    12361 tttgttccat gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt
    12421 agccaatcac cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg
    12481 tgggttaggc ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagataac
    12541 gattcattca aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt
    12601 gttctttttc acagagcgca atggggaaac gctagtggaa gggaatatgt ttcaacgatt
    12661 ggcaaacacc ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta
    12721 cctccatatg agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggtggaac
    12781 atctgggtcg acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat
    12841 gcgttcgatg cttaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg
    12901 cgcagtgttg cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat
    12961 catgatttta tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt
    13021 tgaggtcttc tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac
    13081 tgcaataggg aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac
    13141 tgtggtcttc tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga
    13201 agaaagtgtc atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat
    13261 aagaaaataa aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat
    13321 tttttgaggc actatccgtt gttgaacctc gggtagtgcc tctttcttat tgaaaggaat
    13381 caataagatt tgattccttt atttatcaat aggttataat tcatattttt ttattttttc
    13441 ttttacgata cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg
    13501 aacgaatgca tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc
    13561 gttagcaaga aagtcattct caattatctt aggatcaatc tcaggatcaa ctggaagaat
    13621 tcgatagtaa gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg
    13681 attggctctt ttcgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg
    13741 gaggatttga gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat
    13801 tgcttgttct atcttgtttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc
    13861 agaacaaata aattttgtca ttccagctaa gtagattgtt tggttaggta ataatttttg
    13921 gaaaggagga atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc
    13981 aataacccat agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga
    14041 gatttgaaac cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt
    14101 ttttctacat tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg
    14161 aacgatctct aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc
    14221 tgttgctatt ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg
    14281 aacaccggaa acaatagata gagattctaa ggacggaatg actccttgag tttttaacca
    14341 atttgctgca gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc
    14401 aacaagattt gggttattac tagttgattg aataaacggg acaacgaatt tattacattc
    14461 ttcaaaagaa ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat
    14521 ggtgatggac tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag
    14581 caaacctttc tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa
    14641 ataatcagct agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa
    14701 aatatcttgt tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt
    14761 gtctggtcgc caagttaacg ggtaattgtc ataagaatta ataggcattt tctacactcc
    14821 ttaaaattgt ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt
    14881 tgtgaggagg gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag
    14941 ttttgaagaa gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat
    15001 cagccaaaat cctcgattgg tggaaatttt acctgacgga aaagacaaaa atatgtttgc
    15061 gatgatgcct gcttatttgg gggagaacag atattttgga acaaaagtca ttaccgcttt
    15121 tccggataat catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc
    15181 tcaaaacgga ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc
    15241 tgctgtatct gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt
    15301 aattggagct ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat
    15361 cactacagtg tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc
    15421 taaaaaacag tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg
    15481 tccagcggaa atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg
    15541 ggtaagggaa gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat
    15601 aagtacatcg ttgatggaaa cttctcaaat ctatgtagat gattactcag ctaccttaac
    15661 agaaagcggg gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt
    15721 tggtacatta ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc
    15781 gacgattttc gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta
    15841 taacaaatta gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt
    15901 gcagatgagg tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca
    15961 gaaggtacga tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg
    16021 aagaaaaacc aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt
    16081 ggtcacgcga cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa
    16141 acgattaaat tttctactca aagcggtaat ctatttgtga caagaattaa agattattat
    16201 tacatggatt ttcctagtat tatgccgaaa aaagtgccga tattgactga atatgaagaa
    16261 gcaattgggg ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa
    16321 gatgaaaaaa cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta
    16381 ggaatagagt catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct
    16441 ttccaaaatt aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc
    16501 cttattgggc agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg
    16561 gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt
    16621 tgttttctaa aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt
    16681 ggagagtggc agccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt
    16741 taatgaaaag tttgcgtctt tttttaatta ttggtgtttg ttttttattc aatcaagagt
    16801 aaaatgcccc atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg
    16861 acaaagtagt aagaaggcgt agtgccaggt aatcctttta agtcagtaat ctcaagacct
    16921 gcagctttat gttgctcgtg taatttttct aaatcatcag ctgcaatagc gatatgacca
    16981 tagccgtctc ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag
    17041 tcatctccag gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg
    17101 cggctttctt caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc
    17161 acccgaacac atgtgtgagc cattttcata aattcatctc tccttttcct tttactcaat
    17221 cataacaaaa agaagtaaga aaatagttga tttgtgcttg ttcatgagaa atttctcatg
    17281 taaaatggat agaaaataat actgttaagg tggaaacaat tcatggataa accaattttt
    17341 ggtgaaaaaa agcaatctgc taattatcaa acccgttatg cagcatatat tgtcatagaa
    17401 agagacaagc ggattgcggt gatcgaggct ccaaatggtg cttttttcct tcctggtggg
    17461 gaaatcgaag gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg
    17521 ggaattgtgg cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat
    17581 tatcgtgcaa cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt
    17641 ataggagaac caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt
    17701 gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa
    17761 aactgaaaga aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata
    17821 ttttttgatt gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc
    17881 ttctttgaca agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat
    17941 ggataagtcg ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg
    18001 ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta
    18061 atagggcgca cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat
    18121 tgctgcatct aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg
    18181 tgtcatttct tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat
    18241 cgtataagcg ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag
    18301 atgactgccg acacggcgtg ccatcacttc gatctctagg aaatcttcat gagtcagtcc
    18361 gtgacgggcg atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat
    18421 aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa
    18481 gggacggcca gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc
    18541 ttttacttct tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc
    18601 gactagtttg atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc
    18661 attacggata tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg
    18721 caaggaggga tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc
    18781 cgctaatgca gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg
    18841 caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggcag
    18901 atcgtagcta gaattcacag cagtgtaata aacagggtta tagccaacct tttctactaa
    18961 caaagtgagt tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag
    19021 tggtttacga ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga
    19081 ccattgagta aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt
    19141 aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc
    19201 ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc aaaatcactt ccataccacg
    19261 ggaaacagga tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc
    19321 cactgcatgc atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg
    19381 taatattcgg gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag
    19441 caaatagtcc attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt
    19501 ggggtaagtt ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt
    19561 aagaatctga tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg
    19621 attcgtatgg aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa
    19681 cagtgccgca cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt
    19741 ttcttttgaa aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc
    19801 taatgaatgg gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa
    19861 ttgtttaatg cgtcgtaaac gttggacttc tttcgaattg atcaaatcta agattacttt
    19921 atgctggaca tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat
    19981 ttgatattta taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc
    20041 tctggatttg gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat
    20101 cctgtaaatc acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt
    20161 cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca
    20221 ctgcaggata gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca
    20281 tgacttgccc gcgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc
    20341 catttttgat ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat
    20401 aagttccagg acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag
    20461 cgtatttagt caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt
    20521 cactttgagg gaaaatcaaa gaaatattta aaattccttc atctgatacg atccctaatc
    20581 cacctgaatt tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac
    20641 ttagaaaagg agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga
    20701 aatgtaaaaa gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa
    20761 aaggggaaaa gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc
    20821 aaccggctcc tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt
    20881 tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac
    20941 taggaacaat gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac
    21001 accgatttca atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta
    21061 ttttgatttg aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga
    21121 gattcaggag gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca
    21181 tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg
    21241 gttagaaaca tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga
    21301 actgcatgtt agtttgaaag accgtcccac agcagggaag gttcgaatcg attatgatct
    21361 gttcatggga ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat
    21421 ataaaagaaa agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa
    21481 ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat
    21541 gatcgaagta gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt
    21601 agctaaccag atccaaaact atcttgagaa atcagacagt gaaattcgtg attcactcgc
    21661 acaattttat actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg
    21721 gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga
    21781 cgaagaggat gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa
    21841 tgatgatgaa gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga
    21901 agaagatgac gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta
    21961 taattctgac ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc
    22021 aggaaatatc gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga
    22081 agaggaagaa ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg
    22141 acagagacga tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt
    22201 ttttgtatat aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg
    22261 caccttgtac gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat
    22321 aagaaaaaac ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat
    22381 tatcaaaaaa tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat
    22441 gtgatgcaag atagttttca taaagccttg aactattttg aaaaagaaaa agagatcgag
    22501 cattttagtg catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga
    22561 caaaaaagga cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta
    22621 gaaacttccg ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag
    22681 attctgatcc tctacttttt tgaaggattt cgtttgaaag aaattgcaac tatcttggat
    22741 atgaatgaaa acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc
    22801 ctagaatgaa ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa
    22861 gaaaacttat aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt
    22921 tattatcgaa gagcaatcat ttataaaagc aaggcggaga aagagacgtc tccaagggtt
    22981 cggagtatct ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt
    23041 tcgaagcttt gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga
    23101 acgctttact gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa
    23161 tgagaaagaa aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc
    23221 agattttgaa aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt
    23281 gggagattat cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac
    23341 ccaaacagca gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag
    23401 tgtccagctc tcacttccac ttttattcaa agatgacact tatctatctg ttttgacaaa
    23461 agaagtcaaa cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac
    23521 tgagcaagat attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag
    23581 tttttatttg aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc
    23641 tggttatatg ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc
    23701 atcggaagat tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc
    23761 aagtaagtac aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga
    23821 aattatttag tcatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg
    23881 gtggcctcct tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa
    23941 tctttcaaac aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc
    24001 aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc aatcatcaca atcaacacaa
    24061 gacagtacag aagcaatcca agacccgact gtcaaaagcg tcgcaatcag ctttgatgat
    24121 ggaccaggag cgaccacaac gccgcagctt ctgcgaatct taaaagaaaa aaacgtgcat
    24181 gtcacttttt tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca
    24241 gctgaagcag ggcacgagat tggcaatcac acatacgatc atcagaatct ggccactctt
    24301 tctgcccagt cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaaagtcac
    24361 tggcaaaacc cccgcttttg ttcgtccgcc ctatggttct gtcacgaatg taggagcaac
    24421 gatcatccag cgaccgatca tcgaatggtc tgttgattca gaagattgga agacaaggaa
    24481 tcctgatttg attttgcaga agatccaagc aacagtatac gatgggtcaa ttattttatt
    24541 ccatgatatc tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga
    24601 gcaaggctat cgcatcacga cagttagcga tttattaggg catccaacgg ctgtcgagaa
    24661 ctattatggc agaaacgatc atcggccagt tcagtaagtg agacaagtat ctttaataaa
    24721 taacacattt taataagtag ttgaacacat aactacctta cttgcaaagg cttaacaata
//
LOCUS       DAZKCN010000035        24570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000035 DAZKCN010000000
VERSION     DAZKCN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.035"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG8K3_001292"
     CDS             complement(152..550)
                     /locus_tag="ACG8K3_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9145181.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG8K3_001293"
     CDS             complement(547..1320)
                     /locus_tag="ACG8K3_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9145182.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG8K3_001294"
     CDS             complement(1317..2000)
                     /locus_tag="ACG8K3_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP9145183.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG8K3_001295"
     CDS             complement(2167..3054)
                     /locus_tag="ACG8K3_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145184.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG8K3_001296"
     CDS             complement(3051..3920)
                     /locus_tag="ACG8K3_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145185.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG8K3_001297"
     CDS             complement(4057..5343)
                     /locus_tag="ACG8K3_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9145186.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG8K3_001298"
     CDS             5616..6194
                     /locus_tag="ACG8K3_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9145187.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG8K3_001299"
     CDS             6194..7123
                     /locus_tag="ACG8K3_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145188.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG8K3_001300"
     CDS             7120..8856
                     /locus_tag="ACG8K3_001300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9145189.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8K3_001301"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8K3_001301"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP9145190.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG8K3_001302"
     CDS             complement(9752..10261)
                     /locus_tag="ACG8K3_001302"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP9145191.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8K3_001303"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8K3_001303"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9145192.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG8K3_001304"
     CDS             complement(11562..12653)
                     /locus_tag="ACG8K3_001304"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP9145193.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8K3_001305"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8K3_001305"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9145194.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8K3_001306"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8K3_001306"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9145195.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8K3_001307"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8K3_001307"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9145196.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ACG8K3_001308"
     CDS             complement(16616..17590)
                     /locus_tag="ACG8K3_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP9145197.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ACG8K3_001309"
     CDS             complement(17571..19454)
                     /locus_tag="ACG8K3_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFP9145198.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ACG8K3_001310"
     CDS             complement(19472..21697)
                     /locus_tag="ACG8K3_001310"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9145199.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ACG8K3_001311"
     CDS             21812..22846
                     /locus_tag="ACG8K3_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9145200.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ACG8K3_001312"
     CDS             complement(22913..23926)
                     /locus_tag="ACG8K3_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9145201.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ACG8K3_001313"
     CDS             complement(24004..24192)
                     /locus_tag="ACG8K3_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9145202.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7058 a         5340 c         4397 g         7775 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact
//
LOCUS       DAZKCN010000036        24337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000036 DAZKCN010000000
VERSION     DAZKCN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.036"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..863
                     /locus_tag="ACG8K3_001314"
     CDS             <1..863
                     /locus_tag="ACG8K3_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP9145203.1"
                     /translation="TSVKDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQRILVNIL
                     ARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLA
                     FARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVIL
                     EKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQN
                     DFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMD
                     QSKKENQVTS"
     gene            complement(971..2053)
                     /gene="uxuA"
                     /locus_tag="ACG8K3_001315"
     CDS             complement(971..2053)
                     /gene="uxuA"
                     /locus_tag="ACG8K3_001315"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9145204.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2044..3690)
                     /locus_tag="ACG8K3_001316"
     CDS             complement(2044..3690)
                     /locus_tag="ACG8K3_001316"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9145205.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            3874..4536
                     /locus_tag="ACG8K3_001317"
     CDS             3874..4536
                     /locus_tag="ACG8K3_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9145206.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4680..5243
                     /gene="ahpC"
                     /locus_tag="ACG8K3_001318"
     CDS             4680..5243
                     /gene="ahpC"
                     /locus_tag="ACG8K3_001318"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9145207.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            5253..6782
                     /gene="ahpF"
                     /locus_tag="ACG8K3_001319"
     CDS             5253..6782
                     /gene="ahpF"
                     /locus_tag="ACG8K3_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9145208.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            6939..7289
                     /locus_tag="ACG8K3_001320"
     CDS             6939..7289
                     /locus_tag="ACG8K3_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP9145209.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            7388..7918
                     /locus_tag="ACG8K3_001321"
     CDS             7388..7918
                     /locus_tag="ACG8K3_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9145210.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            8072..9223
                     /locus_tag="ACG8K3_001322"
     CDS             8072..9223
                     /locus_tag="ACG8K3_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP9145211.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9332..11371)
                     /locus_tag="ACG8K3_001323"
     CDS             complement(9332..11371)
                     /locus_tag="ACG8K3_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP9145212.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(11429..11986)
                     /locus_tag="ACG8K3_001324"
                     /pseudo
     CDS             complement(11429..11986)
                     /locus_tag="ACG8K3_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(12134..12300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(12525..12731)
                     /locus_tag="ACG8K3_001325"
     CDS             complement(12525..12731)
                     /locus_tag="ACG8K3_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145213.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(12747..13361)
                     /locus_tag="ACG8K3_001326"
     CDS             complement(12747..13361)
                     /locus_tag="ACG8K3_001326"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9145214.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(13508..14191)
                     /locus_tag="ACG8K3_001327"
     CDS             complement(13508..14191)
                     /locus_tag="ACG8K3_001327"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9145215.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(14300..14758)
                     /locus_tag="ACG8K3_001328"
     CDS             complement(14300..14758)
                     /locus_tag="ACG8K3_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9145216.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(14842..16605)
                     /locus_tag="ACG8K3_001329"
     CDS             complement(14842..16605)
                     /locus_tag="ACG8K3_001329"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9145217.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(16826..18286)
                     /locus_tag="ACG8K3_001330"
     CDS             complement(16826..18286)
                     /locus_tag="ACG8K3_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145218.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(18494..19420)
                     /gene="glsA"
                     /locus_tag="ACG8K3_001331"
     CDS             complement(18494..19420)
                     /gene="glsA"
                     /locus_tag="ACG8K3_001331"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9145219.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(19443..20870)
                     /locus_tag="ACG8K3_001332"
     CDS             complement(19443..20870)
                     /locus_tag="ACG8K3_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9145220.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(20901..22115)
                     /locus_tag="ACG8K3_001333"
     CDS             complement(20901..22115)
                     /locus_tag="ACG8K3_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP9145221.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(22201..22428)
                     /locus_tag="ACG8K3_001334"
     CDS             complement(22201..22428)
                     /locus_tag="ACG8K3_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145222.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(22456..22698)
                     /locus_tag="ACG8K3_001335"
     CDS             complement(22456..22698)
                     /locus_tag="ACG8K3_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9145223.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(22730..23287)
                     /locus_tag="ACG8K3_001336"
     CDS             complement(22730..23287)
                     /locus_tag="ACG8K3_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9145224.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(23300..23488)
                     /locus_tag="ACG8K3_001337"
     CDS             complement(23300..23488)
                     /locus_tag="ACG8K3_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9145225.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(23501..24067)
                     /gene="amaP"
                     /locus_tag="ACG8K3_001338"
     CDS             complement(23501..24067)
                     /gene="amaP"
                     /locus_tag="ACG8K3_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9145226.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         7149 a         4789 c         4240 g         8159 t
ORIGIN      
        1 caacttctgt gaaagacgta caaaaagaat tcacagagta tgaatatatt gactttttta
       61 ttgatctctc tgtagatatt ttgcgaccta atctgccaat ttgtcgcgaa caacgcatct
      121 tagtgaatat tttagctcga ctttctatca cgaaaatcga atcggaagaa acaagcttgg
      181 agcaatacaa acttttaaaa aaagcaaacg ttcctcatgt tctttattca agagaaatgg
      241 tggagtccct ttgtactgta tttgatttgt ttatacctga aaaagaaaag attctttact
      301 cattggcatt cgcacgaaat aaactttata atggatttct taacttcaat caaccaaata
      361 cgccccttcc tactttcatg ctttatcaag aacatagcgt attgaaagaa gtccaaaccg
      421 ccattgaaca attttatatg gatttcagag aacaaaatca acacttgtta ccagttattt
      481 tagaaaagga aaatgtgcaa tggatggttg aagacctagt acatttgtat gatcgctaca
      541 aaaaacagcc gcgaattgtg attggtgtga actatacgcg tgatttttat atcagtaaag
      601 atctgatcgt taagatagaa caaatttttt caaatgaatc gatcaaaata caaaatgatt
      661 ttatggagaa ctgcaacatc gttatcagcg actgcccttt gaaacaattg ccttctcata
      721 tcaaaaaaat ctatatttta aaaggaatca tcacaccaga agactggaaa aaagtgatta
      781 tgaggatctc agaatatatc tttgaattaa aagaagaagc actcatggat caatcgaaaa
      841 aagaaaatca agttacttcc tagaccaaat aagatacata aatccgaatg cttattaaca
      901 acctccggat ttatgtatca agaatttgaa catccttctt atttacataa taaaattaca
      961 gtttttttca ctaattatct ttacttttgt ttcctttttc gattgcttcc cataatccat
     1021 ttagataaac agcacctaat gctcgatcat ataagccgta tcctggtctt cctgtctcat
     1081 tccagatcat tcttccatga tcaggacgga tgtatccgtt aaatccgttg tcatacattg
     1141 tttttagtat gcggtacatg tctaatgaac cgtattcgga tttatgtggt gcctcataga
     1201 aatctcgatt agctaaaaat ttaatatttc gaacatgtgc aaatgggatt cgatctcttt
     1261 ggcaaaattc tgctaaaatc ccatatacat cattttctgg atcttctgca atactccctg
     1321 tacaaagagt gattccgtta taagggctgt ctactatgcg acacaaccaa tctaaatctt
     1381 ctcgattctt tacgattcga gctaatccaa aaatcgagta tggcggatca tctggatgta
     1441 cagccatttt gacccctacc tcttcacatg ttgggatgat tgattggaga aaataggcaa
     1501 aattttctcg aagtttttct tcgtctactg atgcataggc ttcaagcaag gatttgatag
     1561 gagctaagcg ctcaggttcc catcccggca atccaaattc attgctggaa tgttcgatgc
     1621 gcgcgacgat ttctcttgga tcatctggaa tatcatcaga tataaaagct aacgtggatg
     1681 agccgtcttc taagcggtag tcaaggtcag atttcaccca gtcaaataca ggcataaaat
     1741 tatagcagat gacttctatt ccatattctg ccaagtttcg gattgtctgc ttatagtttt
     1801 cgatatactg ctcacgggaa ggtaatccaa ttttaatggc atcatgcaca ttgacgcttt
     1861 caattacttt tagtgttaac ccacgagact caatttcttc ttttagttct ttgatcctct
     1921 ccctaggcca tacctctcct actggtatat ccatcaatgt gcctacaatc cctttgaccg
     1981 caggaatctg tctgatttca tctaaggtaa tcttttcttc ctgtgatccg taccatctaa
     2041 aagtcatttc catgataaat ttcctccatg ttcttgtaag taatcctgta atgtttttcg
     2101 tacggcattt ggtcctgcta ccatcttgat gaacatttct tctatttttc tgccaagctc
     2161 tgcttggtaa agatctacac caaaaatttc tttatttgac aaaataggag ccattttctc
     2221 atgaatatgt gatgtatctt gcgtacccag ttgaatatcg gaaagcttat gctgaaggtc
     2281 tgtcaatagt gggtctggac tcgggatgaa tggttttcct tgatcatcaa tgccaataag
     2341 gtatcgaagc caagcagcga gagtcaatgg tatgaataca agtgtctgcg ggtctttaac
     2401 ttctatttcc atatattttt taatcgtctc accataacga atcgccacct tttgagaggt
     2461 atccgtggcg attcgctgag gcatattagg aatatttgga ttagaaaagc gcacagtaat
     2521 tacttcttcc agaaagatct ttggatcaat gatacctgga gactccacaa ctgggaggcc
     2581 ttcattgtac ccgatttcag aaactagcag cgataaatct ggatctttca tctcctcagc
     2641 tatgctccta taccccagta aacaacccat aatagctaaa gacgtatgca aaggattaag
     2701 gcaagtcgtc accttcatcg tatctgcttt atctaccgtt gcacgatcag tcaagaatac
     2761 cccagctttt tcgaatttcg gacgtccatt gggaaaatga tcttccatga ttaaataatg
     2821 gatgtcctct gtattgacaa atggagcaat attatttcct tttgcggttt ggaaaatcga
     2881 catatttgaa aagccctctt ttgccaacat tttgctaact gacaaggaag gattcggggt
     2941 gatacgatca atcatcgtcc atggaaacgc aacaatttct tcattcgata agtattggat
     3001 aaatgtattt tgtacataac cttccttttg ccattctttt gcgatcgtta gcaaagaagt
     3061 acgtaattga ctaccgtttt gtgaaaaatt atctgtagag acaagtgcaa tcggccaagc
     3121 tccagataaa aaacgtttat aaagtagcac agcaagaata ctcattgtat gtgatggctt
     3181 ctttggacca ttttttatat cgactgcaat cggtcctata tatttattat ttgtatcttt
     3241 caatcgataa cctttttcag taatagcaaa agtaactaat tgtaaactag gattttcaaa
     3301 gtgtcgaatc atcttatcaa agtgttcaga acaatctgga tgacaataca gactgtcagc
     3361 tgtcgcagct aatatacgtt tgacgaactc tccactgctt ggcatgacca cttcaagaat
     3421 caaatcatca aaatcttggt aagccctttc aattatttct tcatcgaatg tttcacaaac
     3481 aatgacacca gtggacaatt cgcctagatc cgctaaacgt tgagcgattt ctgcatgaaa
     3541 tcctcgataa agatttccgc caccgaaatg gatccagaca ggattttttt gtccattttc
     3601 tcttaatgat gcacggtcaa aatgaggtat ttctacttct ttttgagata attcagggat
     3661 aaagttcctc cagttgttta aatcaatcat ttttcccctc ctaaactaac atattagttt
     3721 ttgtattcaa gtgaatttta tgcaataaaa atcgctatgt caaccaaaat tttactattt
     3781 atacaaattt actagttaat gtgacatacc agattgtttt tcagacctct tctgttatac
     3841 tacgttcata gaaatgattt tggaggaata aagatgaaaa gcagcttgca atcacaagct
     3901 tatcaagcga ttcgtaaaca aattatctat gctgacttag aacctggaag gaaaatatct
     3961 gaaaaggcac tggaggaaca tcttgctatc ggccggactc ccattcgcga agcattgatt
     4021 caactacgtc agcaagaact cgtgtatacg atacctcaaa gtggtacgta tgtttccaaa
     4081 attaatcttg tttctgcaga aaatgctcgc tttgtccgag aacagttgga agagcaaatc
     4141 atgattgaat gttgttccaa attggacaca caaacacgaa tagtgctaga aacgatttta
     4201 gaagaacaag aaaaagcagt caaaataaat gacgaacgcg cttttttccg tgcagataat
     4261 ttatttcatg aagcttgttt cgaaattgca ggaagaaaag aagtttggaa ctggttagat
     4321 gaccataata cccatttaga acgtttccgc tggttacgag tgaccattca ggggcttcca
     4381 tgggaaacga tcatggaaca acattatgat ttactcaagg ctttagttga taaaaaacca
     4441 gaagaagctc gatttcaaac gggattgcac ttacacatga tgctgaaaga acaggaaact
     4501 gttactcaac gttatcccga gtatttccag cagtagcatt ttcttgcgac ctcttctatt
     4561 tttataatta accttcgttt ttgagtaatc tttattaaat aacaaaatat ccattgactt
     4621 tgcaactcca actaaattac aataattata aataaataat tattattgga ggttttacta
     4681 tgtcattgat tgggaaaaaa atcgaagaat ttaatgcaca tgcttatcat caaggagaat
     4741 ttttagaagt cagtgaaaaa gatcttatgg ggaactggag tattctttgc ttctatccag
     4801 ctgattttac ttttgtttgt cctactgaat tagaagactt acaagaacaa tacagtacat
     4861 tgaaatcttt aggtgttgaa gtcttttctt gctctaccga tacccacttt actcacaaag
     4921 catggcatga tacatcagat gctatcggta aaatcgaata cgtgatgatc ggtgatccgt
     4981 ctcatcagat ttctcgtatt tttgatgttt tggatgaaga gcaaggatta gctcagcgag
     5041 gaacatttat tattgatcca gacggaatcg ttcaagctat ggaaatcaat gcagatggaa
     5101 tcggtcgtga cgctagtgca ctgattgata agattcgcgc tgctcaatat gtacgtaccc
     5161 acccaggtga agtttgtccg gcaaaatgga aagaaagtgg cgaaacattg aaaccaagtt
     5221 ttgatcttgt tggaaagatt taggtggtgg atatgttaga caaagaaact aaacaaaatc
     5281 tcgaacaata tttagcactg attgaatctc ctattgtttt ttctgttagt ttggataata
     5341 gtgaaaatag tcaaaaacta gctgaattta cgaaggaaat agcagaaatg tctcctaaaa
     5401 tctcttggga aaaaagagat tcgatgcgca cacctagttt ttctatcaat ccagttggca
     5461 aagaaagtgg aatcgttttt gctggtatcc ccctcggtca tgaattttcc tcttttttac
     5521 tggctatgtt acaaattagt ggacgggcac cgaaaatcag tgaatctttg agtcttaaaa
     5581 tcaaaaaaat aacacaaaca ttgcattttg acagttatgt tagcttgact tgtcataatt
     5641 gccccgacgt tgtacaggca ttgaatatat tagctgtatt aaatccaaat attactcata
     5701 cgatgattga aggtgggatg ttccaaaaag aaatcgatga taaaaaaatc atggcagtgc
     5761 caactgtgtt tttaaatgga gaagaattta gtagcggacg catgacgatc gaacaaattg
     5821 ttgaaaaaat cactggccct ttgattgaag aagagttgtt tgaaaaagaa ccttatgatg
     5881 tattagtgat tggcggtgga ccagcagcta gtagtgcagc tatctatgca gcacgtaaag
     5941 gaatccgtac aggacttgtc gctgattctt tcggcggaca agtcgttgaa acactaggga
     6001 ttgaaaatat gattggtacc tcttacacag aaggtccaaa attgatgcag caagtagaaa
     6061 gtcatgtgcg tagttaccct atcgacatta tgatgaatca acaggccgtt tccttaaata
     6121 aagagcaaca ttttattaat attggcttag ctaacggtat atcacttaaa gcaaaaaccg
     6181 ccatcatcgc gacaggtgcg cactggcgtt ctatcaatgt accaggcgaa aaagagttta
     6241 aaaataaagg aattgcttat tgcccacact gtgacggccc tttatttaaa ggaaaagaga
     6301 ttgctgttat cggtggtggt aattctggtg ttgaagcagc aatcgatttg gctgggttag
     6361 caaaacatgt atatgttcta gaattcttac cagaattaaa agcagatcaa atcttacagg
     6421 ataaactagt ctctcttgcg aatgtcactg ttatcaaaca tgcagcgacg aaagaaatca
     6481 gcggtacgaa tcaggtagaa tctctctcct atatcgatcg ccgtacgatg gagatgcata
     6541 cactagctgt ttcaggagta ttcattctag tcggcttact ccctaatacg gattggctag
     6601 atagaacgat cgaaatgaat ccaagaaatg aaatagtgac cgagaaaaat ggcgcaacga
     6661 atatccccgg cgttttcgca gctggtgatt gtacagatag tccatacaaa caaatcattg
     6721 tttcaatggg ttcaggagct actgcagctt taggagcatt cgattatctg atgcgcactt
     6781 gagctatgaa gattgtaaaa agtcagagag cggaaaaaag gatggtgaca agaattttgt
     6841 cacaatcctt tttgtttttt tgaataatcg ctttttcttc tctttttctt atatactata
     6901 agtagtaaat aatttgaaaa aaagaaaggt gactgattat ggcgcaaaat cgtattttta
     6961 cgacaagttt tgcaagcgtt tatcctatgt atgtagcaaa agcggagaga aaagatcgta
     7021 taaaagagga agtagatgaa atcatttgtt ggctgacggg ttattcccaa acagaactag
     7081 aatatcaact acaaaaacaa gtgactttcg aacaattttt tgcggaagcc cctgtcccca
     7141 atccttcacg ttccttgatt acaggtgttg tttgtggtgt acgagtagaa gacatcgagg
     7201 ataacttgat gaaagaaatt cgttatttag ataaaatgat cgatgagcta gcaaaaggca
     7261 aaaaaatgga aaagatcctc agaagttgat ttttccagag atattcttct ccttcgctca
     7321 ttttgtaaca tttaatttgg taaaatgact agttcacgtt aaaataagtc aaaggaggtt
     7381 ttcttatatg tttgagacga ttaaacaaaa tattcaaaaa tacgccttat taagagctgc
     7441 tgtttatatt atttctggga ttgcgattgt gctaaaccct tctgctgttt ttcattttat
     7501 cggttatctg atcaccgcct atcttgtttt gcttgggttg ttgaatcttt ttgaggctta
     7561 ccgttttaaa aggaaaacag gttcttggag tatcggtctg ttcagtggtc tttttttcct
     7621 gatcttagct ctgatcgtct atgcttttgc acctgcaatc gtttctatcc tgccgattct
     7681 tcttggatta gctgttatca tcaacagctt gttccagttg ttcttcagca tgaatacaaa
     7741 agctaaaagt tggagtgttt atagtagtat tctgttgttg attggcggat tcgtcttatt
     7801 gttcaatcca ttcaaaagtt tgatgcttct attccaaata ttcggcttta ttttgatttt
     7861 catgggcatc caggagatta tcaatttcat cagaataaga aaaatgacta tacgttaatc
     7921 atttttagtt attaaaatag accgtataaa aaccaacaat ccaaaaaaat cttttggatt
     7981 gttggttttt ttgtgagtat attttcctat tttttcaata atgtatgtta tgctaagttc
     8041 gggctatatt ttatacaaag gaatgatgag aatggaattt attggtattt tatgcctgat
     8101 attgatttca acaacgattg gctcacatat ctcgcggaga ttaggtatcc cagcagtgat
     8161 tggacaatta cttgttggtg ttttgctagg ccaagctgga ttgaattggg tcaaacctga
     8221 tattctagtc catgactttt cggagattgg tgtgatctta ttaatgtttt tagctggtct
     8281 ggaaagtgat ttatctcttt tgaaaaaata tttccgacct ggtatgttcg ttgctttgct
     8341 tgggatcatc ttcccggttg tttttggctg gttgacaggc aaagcattcc aagtttcggg
     8401 aaatgaagcg attttctttg ggattattct tgcggcaact tctgtcagta tttcagtaga
     8461 agtattaaaa gaactaaacg tagtgaatac aaaagaagga tcaacaattt tgggtgcttc
     8521 tgtggtagat gacattttag tcgttttagt tctaagtttc agcctatctt ttctaacggg
     8581 ggaagcttct agcaatgctt ctttaccgct tgttttgatt gaacagctaa tttattttgt
     8641 ttttattttc ttattagtca aatggattgc gcctttccta ctttcatttg cagaaaaagt
     8701 gtacgcaaat tcttctatta tcatcgtatc gctagttatt tgtttaggaa tgtcttacgt
     8761 agcggatctt gttgggctta gctctgtcat tggtgctttt tttgctggga tagctgtcag
     8821 tcagacaaag gttaaagaag aagtatacca caatgtagag gctttaggat atgcagtatt
     8881 tataccagta ttttttgtca gtgtaggatt agaagttgat ttttctgact ttagtgaaca
     8941 actactcttt atttctatat tgacgcttgt agcgattttg accaaattgc ttggaggtta
     9001 ttttggtgct cagattgccg gtttttctca acatagttca ttgatggtcg gtgctggtat
     9061 gatttcgcgt ggagaaatgg ctttgatcat tttacaaatt ggacagcaaa gtcaattgat
     9121 cgaaccacag tactattcac ctttagttat cgtgatttta ttaagtacac ttatttctcc
     9181 acttgttctc aaatatttta caaaaaaagt ttatgaagtg tgagtaaatc aaaaagaagt
     9241 tgtgaaagag acatcgtgaa aaccgtttat ttgaaaaaag gactgtgacg ataacgccac
     9301 agtcctttta ctgtacttaa aaagcaattt tttattgata attttggttg aggtattgca
     9361 atagttccga tgctcgtttg gttgctgaat catcatcttc tttattttcc aacatgctga
     9421 ccatcatata accttggtta tcaagattga aagcaaacaa gaaactgttc tcttgcccct
     9481 ttacatcctg tttttctttg atttccgctg taccagtttt cgctgccaac ggaatcccta
     9541 aagcagaaag agaatgtgct gtaccattta catcttgcac aacttctctc agatctggca
     9601 cgatcgtttg tactgctgtt tcgctgatga cattcttctt atcttttgtc tctttatctg
     9661 caatcaattt aggatagaca agtgtgccat tgttggtaaa aacagaatac attgctgctt
     9721 gctggatagg agtaattaga agttcgccct gtccatatcc agtatcagct agcaagatat
     9781 ctgagttaaa gctatcttca ttagaaattt gtgctggatt catactaatt ggcaaatcaa
     9841 ggtcttcacc aaaaatgaat ttatctaagc ctgtacgaaa ttttttctca cccattttca
     9901 acgtttcttg tgccgcatat atattatcgg aatagatcaa agcagttttt aagtctactt
     9961 gtgatacatc actactgaca cgcgttgttt taaaagatcc ccaagaactg tctttttgcc
    10021 atttaagccc gttgatcgtc aacacttcat ttggatcgat agtgccgttg tcgagaccga
    10081 ttgctgctgt aatcatttta aacgtagatc caggagcata acctgtcgca aatcggctga
    10141 tgaatggatg ttcaggattt tcttcataag ctttgtaatc ttcttgtgag atcccgtttg
    10201 tcattttgtt tggatcatag cttggagagc tagcaagcgc aagaagatca ccggtttttg
    10261 gcgttgtcgc aacagttgat gcagcttttc ctcctagact gtcaaaagct attttttgtg
    10321 ccttcgcatc gattgtcaat ttgatatctt tgccattttg aacttcatgt tcgatcagaa
    10381 cttttttctc tacaccgtct gcatctgtaa tgcttaattt cccacctgtt gtcccacgaa
    10441 gatccttatc aaaagccatc tccaaacctg agcgaccgat ttttccatta ctgcttaatt
    10501 ctggattctt atcaatatct tctgctgtga tatctcccac gtaaccaatc agctgagcag
    10561 ctgcttcacc caaaggataa tatctgccgt ctacttcttg aatggtagct ccagctggaa
    10621 gttctggcgt tgctccatca atgattttca atgggacaaa gtaatcgggt tgtacccagc
    10681 tttgtgaaat cgcctgattg atagcatctt ctgttaagtc gaaggaggaa gcaatcgctt
    10741 tgatattggc tgttttttcg cctccatctc caagtttact tggaacgact cctaattgtt
    10801 ttagtttgcc tgttgttgct aatggttcac catttcgatc tataatattc cctcttgctg
    10861 cttcttgtgt ggtcaggctt actttgtcat ttccttccat ttctggaaaa accaagttgg
    10921 gttgccagtt gatcgtggtt tgaccatcat ttctgtctaa tgttccttta taagaaagat
    10981 ctttcaattc acctaatgag gtattcatct ttgctttata tgaaaaactg taagtagaat
    11041 cgtctttttt atctactttt agattcgata tctgaagtcc tttgacatca gcagcaccgt
    11101 atatattctg gtatttatct aagatctctt tttcagataa tgcactttta tttgccgcct
    11161 tttttgatgt catttctgca gctttgttat aatctccctt gttcaaagct tggacaaatt
    11221 gctcaactgt cttttctcca gcttctactg cttgggtttc ttgatagtgc tggtagtaaa
    11281 aataaccgcc acccgcagct gctattaaga tcactgcgcc ggcaatataa gcgcctgttc
    11341 gatttttgcc gtgcttgtca cttcttttca ttttttcctc cttctcttgc ttgtcattcc
    11401 tttatttcac tatttatacc tgtttattct aacatatgtc tttgaggaaa tccataccac
    11461 aatccagaag cacactttct ggaaggttcg tttggatact accaagtaat ttcccaaccg
    11521 ctttgggtag cttgaatatt gaagttacgt ctttcagctg ctgagagata gctttgatca
    11581 cttgctgctg ccttctgatc ctcccgaagt ctccttcttt gtcttcccga aatcgagcat
    11641 actggagtaa agtattacca tccatcactt gctctccttt gtttatatat acgccatcta
    11701 aatctagaga tttttctgca tcgatcttga ctccaccagg aaacagttca ttaacgcaat
    11761 cgataaaatc ttgaaatgtg atacttgcat aatagggagc ttcaaatttg aaattttcct
    11821 ttaatgtttg atttaataaa tcaactcccc cataagagta agctgcattt atcttattca
    11881 ttccgtaacc tggaatatcg acatatgaat ctctcatgat agagatcagt tttgcttgtt
    11941 gtgttttctg gttgtattgt gcaaccagaa gtgaatccga gcgacctcca ccaatcgatc
    12001 ttttttcacc gcatctcctc ctcacacagc aatccacttg tcaggtgagg agcgagtttg
    12061 tctcatcaaa taagtcagtc acaaaaatat aaagaaaagg tcagaaacct tttcgaacaa
    12121 gaattctgac ctagcatcat ctcatcgtcc gagttttagc actatacaac gtaaagaaaa
    12181 cattcttagc tattggaaaa agccttaatc caacagtttc tgttttaccc attggcgact
    12241 ttcgacgttt ctgggcaaca gcctatgttt gtataggagc ctcacctaac aaggttatta
    12301 ctatattcta tttacgagtt catgatagac ttttcctaat gattaggcaa tggaatttga
    12361 aatgaaatag tgaaatttaa caatctttaa tattttttag aaaaaaataa caaataagtt
    12421 gaaagaatcc cgttgcaaat tctttcagtt gcaccaggat tctttgataa ttttttagtt
    12481 ttatgtgttt ttttatatgt tttttcactt ttcggaataa atatttattt gttaaagtaa
    12541 ttaaacaatc cgctaatcgt ttgatctggc atttctattt ccttaccatc taaagtaaca
    12601 gtagtaacga caaaatggtc ttgcaatggc ggaacttcta agtactgccc ttgcagaaaa
    12661 tcattcactg tttcatattc ttttttcatt tcttttgaat ctgtcgatgg tttatagctt
    12721 atttttaaca taactagtgc tcctttttat tcgggacgtg caatcgtgaa aatttctcct
    12781 attgctgcat aatcatttcc tgccaaacgg cttacagcat tcaattttct tggatcgatc
    12841 cttcctgtct tatcatcata gactgtttca tttaaatgat atttctgtac ttctagcaaa
    12901 aataaatcag ctgttacttt atttttattc cgtatctcga catgttcata taaggatgtt
    12961 tcaaaacgaa cttttgcttc tgtcaatgct ggtactgaaa tgattactga atcagttaaa
    13021 gtcaagtcag tcaggtacag ttcgctttcc tcattaggca gattggctgc tgttttattt
    13081 acttcttcta cgttgtcctc atcaaggata tggataacag cttctcttgt ctcaagaata
    13141 ttctttgctg tgtcttttac tgcatcttgt tttctctgga tcgccaagga taagatgggt
    13201 cgattcgaac tgacgatatt gaagtaactg aagggtgcga tattcactat atcttttgct
    13261 gatttagtcg ttactactgc tactggtcta gggatgactg aaccaatcag caacttgtaa
    13321 ttttcacgtt ctgtcaattc tgaaggactc cgttcaaaca tggtttcgcc ttctttctat
    13381 aaaattcgtt aaaaagcaga tcttgaagac aaaatgaaaa gtaatgcttt tttcatcttc
    13441 ttagaatctg ctttaaacta tctatcaatt tttctttttt gtcaagaatc tgtgccgtgc
    13501 gtgtttttta gttatttgct ttacttgcgg cttcgacata tcttttagtg atcaattgcg
    13561 gacgggtgat aatagatcca actacgactg aaaaagcacc taattcatat gctttctcta
    13621 cttgttcagg agagtcagta tgtccttcca taattactgg gatatcgact tgttctatga
    13681 tttgattcaa taaatcaaaa tcaggtgtct gcgtctttcg tgaatattct gtatagcctg
    13741 ctaaagtcgt agacaaaaga tcaaaaccat cttcttgcgc ttgaagcgct tcctcaaaag
    13801 tcgaaatatc tgccatagct aaccgaccgt gttcgtgaat catttggaca agttcagcag
    13861 ttgttttttc tgtcggacgt ttacgagaag ttgcatccag tgcaatgatt tctgcttgcg
    13921 tctccaaaag ttcttccact tctttttctg tcgcagtaat aaacacttct gacccatcgt
    13981 aatctctttt gataatccct attaccggta aatcaacgat tcgcttgatt tcattgatat
    14041 cttctgtcgt atttgctcga ataccaactg ctccgccttc ttttgcagcc aaagccattc
    14101 tccccataat aaaagaactg tgtagcggct cgttttccaa agcttgacaa gaaacgacga
    14161 ctccgccttt gatttgttcc agtgtatcca caatatctcc tccagtaatg ttatataaaa
    14221 atacagcttc atataaaaat gtaacccttt tctattcagg agacaataat gagctatttc
    14281 tttcagcaga cctttcttac taaaagaaaa ttacttcaaa ttcttcgtat acgaccagct
    14341 tctcttctgt aaattctatg ttcaacgctt ctttataagc atgtttaaaa aactcagcat
    14401 ggactttccg ccaataagct aatgtccgat ccccttctcc ttctttccaa gcatgctctt
    14461 tcgtgacttc actaaaaggc aagatggtca catttatatt tttgatgata caaactggtt
    14521 cattttttcc attcaggacc acgtctaatt gaccaacttt tggtaaaggc tcattctctt
    14581 gtttatacag atcgtaacca cttgatgtac cagtctttat cttttttaca actagttcgc
    14641 tcaactcgtc tgccattttt tctgtatccc caaatgaaaa gaaacctaat ttttctgatt
    14701 gataactagg atatttttct tggaactttt tgacatattc ttctacttgc tgatacataa
    14761 catccacccc tttgcttttt ataatgaaaa aaccatccag atactagtat aaacaattat
    14821 ctggatggtg agcataatga attattgaat attcaagaca cgcagactat cttcaacgat
    14881 ttgttttggt gttaaatgat aacgttcata aagctgccct acaggaacgc tatccgtgaa
    14941 ttctttctct gcaccaaaat ttaatacgtt cattgaagaa tcaccataat aacgactgat
    15001 attttcgcca aatcctcctg acaaactacc atcttctaat gtgatgacta attcgtgtgt
    15061 ttcttttaat ttatctaatg tttctacgtc attttctgtg atcgttcctg gattgatcaa
    15121 cgttgcttcg attccttttt ttctaaaagc ttttactgtt tcttttccta attcaagaaa
    15181 accgcctaaa gctaaaattg ctacttttcc tccattattt actgtttgat aggctggaat
    15241 actataatca tttgcgatag ctgtaccatg ttctacacca tgtgtaggaa ttcggattgc
    15301 tacaggctgc gctgtttgtt cgaacgccca atctagcata gagatcatat cctcttttgt
    15361 tgctggagca agatatttga tgttcgggat acttgataag taaggaatgt caaatgttcc
    15421 ttggtgtgtt cggtcactag ctgagatcgt tccgccaccg actaaaataa tcactggatt
    15481 ctcgttgatc gctaaatcat gtgataattg atcatatgcc cgttgcaaga aagtacttga
    15541 ttggaagacg actggacgta ctccctgaga agccaaagca gaagcgaaag tgatagagtg
    15601 ctgttcagcg atccccacgt caaaataatg atctggatgt ttttcttgga atgttttcag
    15661 tccaaaggcg cctggtatcg ctgcattgac tgcggcaata ggagctgtac ttgtttgcat
    15721 tttcttatcc aaataatcca tgatgatcat attaaatgtt tctccagaag ttacttgctt
    15781 tgcctccccg gtggacagat caaatggtac atgccaatga tatttcattt tattatcgat
    15841 tgccggctga taaccgcgac ctttttccgt atggacgtga agtacaatcg gatgatcaat
    15901 atcttttact tcttcaaata aacggatcac cgtttccaga tcgtttccat tatccacgta
    15961 acgataatca aatcccattg ctttaaatag gttattgctg cttttaccgt tggtttgacg
    16021 aagctcagct aagtttttat ataaactgcc atgattctca tcaatagaca tctcattatc
    16081 gtttactaat acgattagat tggattgtaa aacaccacca ttgttcaatc cttcaaaagc
    16141 taagccacca cttagggagc cgtcaccgat aaatgctacc acatttcctg attctttctt
    16201 cagatctctc gctttagcta aacctgttgc taagctgata gatgtagatg tgtgccccac
    16261 tgtaaaatag tcatgtttac tttcatgttg gttggaatat ccgctcactt cacggtaatg
    16321 tcctttttca aaaccatttt ttcgaccagt taaaagctta tgaccataag actgatggga
    16381 aacatcccaa acgattttat cctttggtga atcaaataca taatgaaacg caatcgttaa
    16441 ttctactaca cctagattcg gaccaacgtg cccacctaca tcgctgtcct tttgcaagat
    16501 tttttggcgc atctcttctg ccaactgttt catttcctca acagataatt ttttgacatc
    16561 tgcggggcta ttgattgcat ccaatacatg taatactgta ctcataatac attctctcct
    16621 ccgtactatc ttcttttaat gaaaatagat ttgttacact ttatctattc tactacttag
    16681 agttgactct aagtcaatag cattttaaaa aaatttcaga gaaagcttgt tatataagta
    16741 gatcttcgca cgaatcagga ctcaaataaa aaccttctac agacaattaa aaagctttgt
    16801 agaaggtctc ctaattaagg tctcatcaag aaatatgttt ttttaatagg tgaagttcct
    16861 gagcaatatg atacaaatgg atgtttgcac cttttaatga gattggatag atctttgtct
    16921 tttcagtttc taatccattt tgatagctag cagcatcgca caaaaccact ctttttttcg
    16981 cacatagaca ttcatcaggc aattcttcta atgaacgaaa taaaatagga tttatttcag
    17041 cgatgccttc ttccgcatac aaatgaaatt gggttcgata aaaaaattgt ttgactgaac
    17101 tttctgaaac aacaaaaatt tctatttcct gtctccccgc ttgttcactg ttgatcaaag
    17161 aaagactttt gacgaatttt cgaatgaaat tttcattcca tcttagttgg atttgatttt
    17221 tctttttcca ctcttccaat atttctacaa tttttccgta cagaagttgt tctttttctg
    17281 atagaagata gatcctttcc ggtaaaaata cttgtccatc tgcccacaac gtacggacaa
    17341 attgtaaaaa ggattgcttg aaaataaaat tttctgcatc gtcgatttca gcgatactaa
    17401 taatctgtaa aatcaattca ttcagtgata ggtcattgag aacaaaattt ttataaacaa
    17461 cttcgaaatc ttttcttaac agctccatgt tctcattggt ataatttcta ctgttgaaca
    17521 aagaaaaaat ataatagaca tcttcttctt gtaagtctag aattcctttt tgctcttcca
    17581 atccttgaca gactaattga aataacggct tcgtttctgc gcttttctta tcttcttctt
    17641 tcagtccaag gggaaatgcc tttcttccga ttgcgatctc tattcctcgt atgataaagg
    17701 acttttcatc ttccgaatat cttcttttaa aaaaatgagt ctctacataa tcaatcaact
    17761 cgttacaagc ttcttctaca tacggatttt tattttcata gccttcccaa ttgacataac
    17821 gtactaaagc tagcaagatg ttccgcgagt caaattctgg gataatgatt tctttatttt
    17881 tcaaataatc attttcttta aaaaaatcca atacgatttt tttgattgta tatactttgc
    17941 tcaaagaaat aaattcaaga tcagccagtt ctgtactcgt gaatatacca ttaaaataat
    18001 aacttaaaaa ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc tcagaaagag
    18061 caataccatg tttgaatacg agtttatact tatgcttctc tcttacttgt tcttttatac
    18121 aatattctag acactcggct agactttcta gatcgtgaag gatcgttggt gtagtcaccc
    18181 ttagagaaat agctgtttgt tcgatgtcta tttcttggaa ttcaaataat tgttcacaca
    18241 gatacacttt tctaaatata tttcgctcga tgtaattttc cagcattcct gatacacgcc
    18301 tccagttttt cttatgattt tatttaatca tatctgtaaa tcgtattttt gtttcctttt
    18361 tatttagaaa agccctttca aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa
    18421 gctcacaatt tcagcctcat tgtaggtagc tttgagccgt ttggactttt accccagcct
    18481 ctttcaagaa tggttattca aagatgtcag cgtctagttc tttgactaca tctttcaaca
    18541 attggatacc agctgcgctg ttaccaaatg gatctagtgc cgggctgaag acacctatgc
    18601 cgtaacgatt tggcacagct gccatcaacc cgccgcctac accgcttttt gcaggtacac
    18661 caacatggac ggaaaactct cctgattcat catacaaacc agcagttgtc ataattgatt
    18721 tgacgattgt agcactttct tcagtaatga tttgtttgcc attccaagga gcaattcctt
    18781 tatttgccag cacagcagct aattttgcta tgcctgtagc ggtgacattg agggagcatt
    18841 gtctgaaata agcatcgagt atctccggta cgtctccttc gatcatttga ttacctttca
    18901 tatagtaagc aagagagcga ttgatatcac ctgttcttga ttcagaatga tagatttctt
    18961 cattcaatgt gatattagga tcatcacaga tctctttcat aaatgacaag attcgctcaa
    19021 atttttcatc tgcatcttta ccggcaatca acgaggttgt tgcaatcgct cctgcattaa
    19081 caaaaggatt cattggatgt ttgcgatgat tgatttccat attcatgata gaattgaagg
    19141 caaaaccggt tggttccgtg ttgattgttt gaaagacttt ttctattccg ttgtctttga
    19201 ttgctaataa caatacggga acttttgaca tgctttcgat ggcaaagcgc acatcggcgt
    19261 ctcctccctc aatcagttgt tttgtatcca aatcataaat cgccatacct aattgtttgg
    19321 gatccacttc agctaatgct ggaatgtaat ccgccacttg tcctgtttga taatagtgga
    19381 aattttttcc aatcaattgt tctaattctt ttacttccat ttgaataacc agctttcatt
    19441 ttttattttt ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa tggaataact
    19501 aagacgataa gataagaaac aatcagaatc gtttcataaa tcccatgctg tccagctgcg
    19561 atactagcag gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa
    19621 caagcaacga ttacttttac aacttttccg cctggaacct gataggctct ttcaagactg
    19681 tcatctttaa aaaccagctt caaatagccc gcgaaaaaca taaagtacaa catcaggtag
    19741 attacaacag tcaatgacat cgctgcaaga aatgaaagat tattcccccc gccgcttaat
    19801 gtaaagacgg ctgcccagat tgttgcaacg atcccatttg cgatcatcaa atgagcagga
    19861 acaccgtgtg tgttttcttt tgcaaaatat tcaggaagta atccttcttc tgctgctacg
    19921 tgaagtgctt ttgaaggaga ggttacccaa gaagcaattt ctccgatgac acctaaacaa
    19981 acaagaatcg caagaatttt cgcaatccat gcaagatttg gatcaatgtg tcttaatagg
    20041 aattctaacg attgaatcac ccctgtatta agagacaaac catgttgagg tactgtagta
    20101 gcgacggtca atccgccaaa agtatctaaa atgattgcca gaataaccaa tacaaacatt
    20161 gctaatggat aatccttttt agggttttcc atttcatttg catgactggc agatgtttct
    20221 acgcccatat atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa
    20281 tcaggaatca atgttttcat agacagtatc gtttgtagtg gagcgcctga agcgacataa
    20341 tggatcccta agccaaacaa gactaatgct ggcaacaaga taccagctac aaaaccgatc
    20401 cgagcaatca atgaagtgta tttggttcct tttaactgtg agagtaaaat agcccagaaa
    20461 ataatcaata ggattgccaa tttcagatat ttattgtttt gtatagcacc aatacccgtt
    20521 gcataggata atgcaccagt taagaaatac agcatggtca agtagccaac tgttatctca
    20581 aaccattgga aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa
    20641 gtatagacac cgcctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc
    20701 acagggataa accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag
    20761 gtcgcgaatg tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag
    20821 ccaaagaacg ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct
    20881 cttttctatt tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct
    20941 tcttcttgtt cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc
    21001 actgttgtca gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct
    21061 aagaagtacc agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc
    21121 agaatcgtac ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc
    21181 cattgtttct ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca
    21241 atcagcatgg cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca
    21301 gcaacggatc ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg
    21361 atcagccaag taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact
    21421 gcttgttcat ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc
    21481 agtcctgtac cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga
    21541 atctttcttt gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg
    21601 atgactgctc caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat
    21661 atccagtggc aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag
    21721 ccttttaaat tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata
    21781 aacatcagtt ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg
    21841 gggaagcttg taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg
    21901 atatcccgtc caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca
    21961 acggagagaa aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg
    22021 taaaaaaaag ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat
    22081 gctgtatctc ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta
    22141 cgagtataaa agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat
    22201 ttataaaaga cagacaatta ataaaagctt gatgtgttgt gctctttttc gtttttcaca
    22261 caaataaaga caaaaaataa acggtcttct ttttatagaa aaccgtttat tttcgtcttt
    22321 ttttattttt aaagatattt tttcataagg aataaagaat acttcatata aaatatataa
    22381 aaaagaaccg cagcgagtgt cgctacggtt ctcaaatttg ttcttcattt caacataagt
    22441 ttttcttcaa tttgcttata atttacgtgc aaagaatgaa acgactaata ctaaaatgat
    22501 tgcaccgatc aatgatggaa tgatagccat tcctgcaatt gttggtcccc aagttcctag
    22561 taaagcttgt ccaattgctg aacctactaa accagcaatg atgtttgcga tgatccccat
    22621 tgatgatcct ttattcgtga ttgctcctgc aattgctcca atgataccgc cgacgattaa
    22681 tgcccaaata aatcccattt taaattcctc ctaactgact tcttacttct tattgtacac
    22741 gtggttcgtt attttctttg actttttgtg cacctttgcc tacagcagat tttgctttat
    22801 ctgtttggcg tgaagcaaat tgtcctgtgg attttgctgc ttctgttact ttgtcttgta
    22861 ctgtttcttg atctttttgg tattcttctt gtgttttgat gtctgcaaca ttcacattca
    22921 cttcgataac atctaaatgt gtcatattgt ttacttcagt actgatcaat tctttgattt
    22981 ggttgtaaat atcttcgatg tctttaccgt attctacgat cacatctaga tccacagcta
    23041 cttgtttctt acctacttct gtattgatac ctgctgttac gttatctgtg ttgactaatt
    23101 tttcagccat atttgagaaa aatccgccat ctaatgtcaa tagaccatct acatctttca
    23161 atgcataacc gatgattttt tggatgactt tgtcttcata agtcaattca ccattcatca
    23221 tgcggttttc tcctttttca tttgccaaaa ctgatccatt tgttgtattc atgtttttgt
    23281 tttccatctt tgtttcctcc tatgatttaa aaaattgatc aactattcct gactgtttca
    23341 aataaaaacc gattcctgcg ccaattgctg tcatgattat gatcaacaat gtcttgaaaa
    23401 atccaatcgt taaccataaa acagctagca gtagtccaag acccccaaag ataataggga
    23461 gcttgtatgc tgaaaatagt tctttcatta tttggccctc ctattccact cgagagtgct
    23521 tgtagtctct tgtatcttct tgttcttcat aaccgctgta tgtgactgcc actttgaccg
    23581 tttcttttgt tccgagtact ttagttactt cttcttcgat atcttgcatc aatacacctg
    23641 ttttccctac taatgaagaa gttcgtttca agtctccgtt gacatggata tggattcgat
    23701 ttttcgttga acgtatccgt actttaggtg aatgaatgaa ctcttcttcg tgtaaatggg
    23761 aacgtaccaa cccttcgatc gcttttttgt cgattgttag tttgccatct tcccgtttca
    23821 taacgaaagt tcctcttgac tttggataga aaagaacgac tagcaagatg ataatagcaa
    23881 gaacggcaaa tactacagct gcccagaaaa tatattgttg catgtagtaa ttcgttaaag
    23941 taaagaagcg aaatcgttcc aattgttcag gcataatata cggcgcattg atcaacgcaa
    24001 tcgctagaaa tactgaaagt agcagtaaac tcatgataac accgattatt tttaacccct
    24061 tattcatttt ccacctccta taaacttatt tttaccctgt aaataactct accgacaatc
    24121 acagtataga tagcgaacag ttagcatagc atcagtaaat aaagcgctac caaacattgc
    24181 tggatcataa cggcgtttta ttgccagtct gatactgaaa taacaattgc ttacttattc
    24241 tctatttact tttgattgct ggctatactt aaaggatatt attcaagatt aaaaattgca
    24301 aagattatgc tcagttatta ttacaagatt ctattta
//
LOCUS       DAZKCN010000037        24271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000037 DAZKCN010000000
VERSION     DAZKCN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.037"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACG8K3_001339"
     CDS             <1..572
                     /locus_tag="ACG8K3_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9145227.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACG8K3_001340"
     CDS             576..1217
                     /locus_tag="ACG8K3_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145228.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACG8K3_001341"
     CDS             1214..2131
                     /locus_tag="ACG8K3_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9145229.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACG8K3_001342"
     CDS             2128..2799
                     /locus_tag="ACG8K3_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145230.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACG8K3_001343"
     CDS             complement(3340..3828)
                     /locus_tag="ACG8K3_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP9145231.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACG8K3_001344"
     CDS             complement(4029..4295)
                     /locus_tag="ACG8K3_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP9145232.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACG8K3_001345"
     CDS             complement(4295..4558)
                     /locus_tag="ACG8K3_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9145233.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACG8K3_001346"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACG8K3_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACG8K3_001347"
     CDS             complement(5361..7184)
                     /locus_tag="ACG8K3_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9145234.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACG8K3_001348"
     CDS             complement(7782..10487)
                     /locus_tag="ACG8K3_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP9145235.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACG8K3_001349"
     CDS             complement(10793..11740)
                     /locus_tag="ACG8K3_001349"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP9145236.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8K3_001350"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8K3_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9145237.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACG8K3_001351"
     CDS             complement(12375..12737)
                     /locus_tag="ACG8K3_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9145238.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8K3_001352"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8K3_001352"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9145239.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8K3_001353"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8K3_001353"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9145240.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACG8K3_001354"
     CDS             complement(14365..15876)
                     /locus_tag="ACG8K3_001354"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9145241.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8K3_001355"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8K3_001355"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9145242.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACG8K3_001356"
     CDS             complement(17703..18779)
                     /locus_tag="ACG8K3_001356"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9145243.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACG8K3_001357"
     CDS             18975..20720
                     /locus_tag="ACG8K3_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP9145244.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACG8K3_001358"
     CDS             complement(21000..21437)
                     /locus_tag="ACG8K3_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9145245.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACG8K3_001359"
     CDS             complement(21421..22794)
                     /locus_tag="ACG8K3_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145246.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="ACG8K3_001360"
     CDS             complement(23116..23604)
                     /locus_tag="ACG8K3_001360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9145247.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="ACG8K3_001361"
     CDS             complement(23626..24228)
                     /locus_tag="ACG8K3_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9145248.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAZKCN010000038        24187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000038 DAZKCN010000000
VERSION     DAZKCN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.038"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="ACG8K3_001362"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="ACG8K3_001362"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9145249.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="ACG8K3_001363"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="ACG8K3_001363"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9145250.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="ACG8K3_001364"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="ACG8K3_001364"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9145251.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="ACG8K3_001365"
     CDS             complement(3376..4683)
                     /locus_tag="ACG8K3_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP9145252.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="ACG8K3_001366"
     CDS             complement(4686..5267)
                     /locus_tag="ACG8K3_001366"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9145253.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="ACG8K3_001367"
     CDS             complement(5839..7131)
                     /locus_tag="ACG8K3_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9145254.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="ACG8K3_001368"
     CDS             complement(7128..7751)
                     /locus_tag="ACG8K3_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145255.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="ACG8K3_001369"
     CDS             complement(7754..8590)
                     /locus_tag="ACG8K3_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145256.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="ACG8K3_001370"
     CDS             complement(8607..9494)
                     /locus_tag="ACG8K3_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145257.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="ACG8K3_001371"
     CDS             complement(9510..10793)
                     /locus_tag="ACG8K3_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9145258.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="ACG8K3_001372"
     CDS             complement(11476..12159)
                     /locus_tag="ACG8K3_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9145259.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="ACG8K3_001373"
     CDS             complement(12177..12794)
                     /locus_tag="ACG8K3_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9145260.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="ACG8K3_001374"
     CDS             complement(12804..13628)
                     /locus_tag="ACG8K3_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP9145261.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="ACG8K3_001375"
     CDS             complement(13621..14181)
                     /locus_tag="ACG8K3_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145262.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="ACG8K3_001376"
     CDS             14355..15311
                     /locus_tag="ACG8K3_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9145263.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="ACG8K3_001377"
     CDS             complement(15616..16572)
                     /locus_tag="ACG8K3_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP9145264.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="ACG8K3_001378"
     CDS             16676..17278
                     /locus_tag="ACG8K3_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9145265.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="ACG8K3_001379"
     CDS             complement(17366..18865)
                     /locus_tag="ACG8K3_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9145266.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="ACG8K3_001380"
     CDS             complement(18881..19405)
                     /locus_tag="ACG8K3_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9145267.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="ACG8K3_001381"
     CDS             19703..20452
                     /locus_tag="ACG8K3_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9145268.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="ACG8K3_001382"
     CDS             20443..21228
                     /locus_tag="ACG8K3_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9145269.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="ACG8K3_001383"
     CDS             21197..21913
                     /locus_tag="ACG8K3_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP9145270.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="ACG8K3_001384"
     CDS             complement(22015..23034)
                     /locus_tag="ACG8K3_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP9145271.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24054)
                     /locus_tag="ACG8K3_001385"
     CDS             complement(23053..24054)
                     /locus_tag="ACG8K3_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP9145272.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7160 a         5216 c         4217 g         7594 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaaagctttt attttgctaa tacgtattaa
    24181 cattttt
//
LOCUS       DAZKCN010000039        24154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000039 DAZKCN010000000
VERSION     DAZKCN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.039"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..995
                     /locus_tag="ACG8K3_001386"
     CDS             105..995
                     /locus_tag="ACG8K3_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFP9145273.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1321..3795
                     /locus_tag="ACG8K3_001387"
     CDS             1321..3795
                     /locus_tag="ACG8K3_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9145274.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(3898..5634)
                     /locus_tag="ACG8K3_001388"
     CDS             complement(3898..5634)
                     /locus_tag="ACG8K3_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9145275.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5631..6335)
                     /locus_tag="ACG8K3_001389"
     CDS             complement(5631..6335)
                     /locus_tag="ACG8K3_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9145276.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6466..6969
                     /locus_tag="ACG8K3_001390"
     CDS             6466..6969
                     /locus_tag="ACG8K3_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP9145277.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6929..7267
                     /locus_tag="ACG8K3_001391"
     CDS             6929..7267
                     /locus_tag="ACG8K3_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP9145278.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7288..8706
                     /gene="ffh"
                     /locus_tag="ACG8K3_001392"
     CDS             7288..8706
                     /gene="ffh"
                     /locus_tag="ACG8K3_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9145279.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8818..9396
                     /locus_tag="ACG8K3_001393"
     CDS             8818..9396
                     /locus_tag="ACG8K3_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9145280.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9400..9921
                     /locus_tag="ACG8K3_001394"
     CDS             9400..9921
                     /locus_tag="ACG8K3_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP9145281.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10000..10554)
                     /locus_tag="ACG8K3_001395"
     CDS             complement(10000..10554)
                     /locus_tag="ACG8K3_001395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9145282.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10807..11082
                     /gene="rpsP"
                     /locus_tag="ACG8K3_001396"
     CDS             10807..11082
                     /gene="rpsP"
                     /locus_tag="ACG8K3_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9145283.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11094..11345
                     /locus_tag="ACG8K3_001397"
     CDS             11094..11345
                     /locus_tag="ACG8K3_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP9145284.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11469..12260
                     /locus_tag="ACG8K3_001398"
     CDS             11469..12260
                     /locus_tag="ACG8K3_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145285.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12430..12951
                     /gene="rimM"
                     /locus_tag="ACG8K3_001399"
     CDS             12430..12951
                     /gene="rimM"
                     /locus_tag="ACG8K3_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9145286.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12941..13702
                     /gene="trmD"
                     /locus_tag="ACG8K3_001400"
     CDS             12941..13702
                     /gene="trmD"
                     /locus_tag="ACG8K3_001400"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9145287.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13838..14185
                     /gene="rplS"
                     /locus_tag="ACG8K3_001401"
     CDS             13838..14185
                     /gene="rplS"
                     /locus_tag="ACG8K3_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9145288.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14495..14737
                     /locus_tag="ACG8K3_001402"
     CDS             14495..14737
                     /locus_tag="ACG8K3_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP9145289.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(14825..14971)
                     /locus_tag="ACG8K3_001403"
                     /pseudo
     CDS             complement(14825..14971)
                     /locus_tag="ACG8K3_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            15028..15342
                     /locus_tag="ACG8K3_001404"
                     /pseudo
     CDS             15028..15342
                     /locus_tag="ACG8K3_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            15371..16630
                     /locus_tag="ACG8K3_001405"
     CDS             15371..16630
                     /locus_tag="ACG8K3_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9145290.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            16683..17579
                     /locus_tag="ACG8K3_001406"
     CDS             16683..17579
                     /locus_tag="ACG8K3_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145291.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            17590..18459
                     /locus_tag="ACG8K3_001407"
     CDS             17590..18459
                     /locus_tag="ACG8K3_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145292.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            18469..19144
                     /locus_tag="ACG8K3_001408"
                     /pseudo
     CDS             18469..19144
                     /locus_tag="ACG8K3_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            19350..21014
                     /gene="alsS"
                     /locus_tag="ACG8K3_001409"
     CDS             19350..21014
                     /gene="alsS"
                     /locus_tag="ACG8K3_001409"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP9145293.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            21027..21737
                     /gene="budA"
                     /locus_tag="ACG8K3_001410"
     CDS             21027..21737
                     /gene="budA"
                     /locus_tag="ACG8K3_001410"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP9145294.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            21867..22364
                     /locus_tag="ACG8K3_001411"
     CDS             21867..22364
                     /locus_tag="ACG8K3_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP9145295.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            22549..22809
                     /locus_tag="ACG8K3_001412"
     CDS             22549..22809
                     /locus_tag="ACG8K3_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145296.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(23171..23410)
                     /locus_tag="ACG8K3_001413"
     CDS             complement(23171..23410)
                     /locus_tag="ACG8K3_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145297.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            23696..24010
                     /locus_tag="ACG8K3_001414"
     CDS             23696..24010
                     /locus_tag="ACG8K3_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145298.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8012 a         4120 c         4994 g         7028 t
ORIGIN      
        1 tttacatgat ttttacataa cttaactata tttttacagc aaccatacga gggatttaca
       61 gtcgactgat aaactttagt tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt
      121 ttactctagg tatgatggga attcttttag ccggatgcgg ctcaggatca ggaaatacag
      181 atggctctgc cacacaaaat aattcaagtg actcaaatga acaagtaaaa atcgttgcgg
      241 taggttcgac agcattacag ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa
      301 atccaaatta tcaaatctct gtacaaggag gaggaagcgg aactggtttg agccaagtcg
      361 aagctggagc agttactatc ggaaactctg atgtatttgc agaagaaaga gacggagttg
      421 atgcttcaaa attagtggat cataaagtag cagtggtcgg gatggcacca atcgtcaata
      481 aagatacaga tgtcaaagat atcaccaaac aggaattgat tgatatcttt acaggaaaaa
      541 tcactaactg gaaggaagtt ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa
      601 acggcagcgg tacccgcgcg acattcgaaa aatggggact ggatggtgct acccctgtgc
      661 agtcccaaga acaagattca tcaggaacag ttcggcagct ggtaagtcaa acaccaggag
      721 caatcagcta tttagctttc tcatatctcg atgattccac acaagcacta agtatcgacg
      781 gtgtagaacc aaaagaagag aatgtagcag ataacagctg gggaatctgg tcatatgaac
      841 atatgtacac gaacggaaaa ccttcccctg aagtccaaaa attcttagac tacatgatga
      901 cagaagaaat ccaagaagga cctgtcaaag aattgggcta tttaccaatc acaatgatgg
      961 aagtagaacg tgatcacgaa ggaaatatca aataactagc agataaacat gccacggtag
     1021 tcgttactat taaggaaaaa tcaactatgt ttccccacat atacattttt cctttaattg
     1081 tgaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
     1141 aacaccgttt attcgaaaaa aagagctacg acatagaact gtcgtagctt tttttttgct
     1201 atatgacagg aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt
     1261 ctatactgga taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac
     1321 atgaagaata aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat
     1381 aaagtcatcc gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa
     1441 cgaatagaaa aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa
     1501 atcgtattgg aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg
     1561 ctttgtttag tagaatccta taagaacttg acttttttac cagttatcat agccaataca
     1621 ggaatcgcca tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta
     1681 aatacagcaa aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac
     1741 cttgtaatag atgacattgt tattttagag actggacatc aaattccagc agatgcagag
     1801 gtcgtggaag ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata
     1861 accaaagaaa taggcgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac
     1921 gcccgattag acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag
     1981 acaatgggag aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg
     2041 attgggatca tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat
     2101 ggaaacacga tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct
     2161 gaaggactgt acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag
     2221 caaagagttc ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt
     2281 tgtgtagata aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa agtgatcgtc
     2341 tctaaaaaac aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat
     2401 gcaaaagcga tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa
     2461 accactgatc aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt
     2521 gtagcgttgg cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa
     2581 ttgccggaat atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc
     2641 tttggtgtgt acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta
     2701 ggctatattc tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc
     2761 aaaaaacagc aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat
     2821 gtagctgtcc aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca
     2881 gaagaagaat atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa
     2941 caaaagaaaa tcttcgttca attgctaaag cgtcgtaaca atacagtagc tatgacaggt
     3001 gatggcgtga atgatattct agccatgaaa gaagcagact gcagtatagc gatggcttcc
     3061 ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac ttgattctga cttttcacgc
     3121 atgccagaag ttgttgcaga aggacggcgc gttgtgaaca atattgagcg atcagccagc
     3181 ttgtttttag tgaagaatat tttctccttt ttgctctcac tgttttcagt gatctttgct
     3241 ctgacctatc ctttagaacc atcacaaatc acattgatta gtttgtttac tatcggactt
     3301 ccatctttct tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa attcatcatg
     3361 aatgtgatgg aaaaagctgt gccaggtgga ctaacagata tgatcgtagt cggtgcgttg
     3421 gtgatctgcg gggtgacttt gaatctaaat aaaacagatg tttctacagc atctaccatg
     3481 cttttgattg cagtagggtt cctcgttctc tataaaatct gttcaccgct gaataaattc
     3541 agaagtcaga tcattctttt ctgtgctagc ggtaactttt tttcggtcat ctttttgcat
     3601 aagctgtttt caatcaccgg tatctcagct gtatcacttt tgctgttgag cattctattt
     3661 ttctcggcag attcgatttt taggcatttg accacattgg tagaatattt gtttgaacgt
     3721 aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagtta aaaaatttct atcgatattt
     3781 aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt
     3841 atctttcgaa gcaggatgag tcatccaact ttaaaacgca cgagcagttg ctctttttta
     3901 cattttcgga atggtaacgg tgaaagtcga acctgttcca agatgactgt ctacagtaat
     3961 cgtaccatga aggagttgca cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc
     4021 tgaatgccgg cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc
     4081 ctcaatgtcg atacctatac caaagtcttg gacagaaaac gatagatcat ctgttgcctg
     4141 atagcggata ataatctcgc tgtcgctttt tgaatattgg acagcatttt caatcagatt
     4201 tttgataatt ggataaagga tatctgtttt agtagtaaat ggttcgttct ttccaatcaa
     4261 tcgaatcgtc aaccgtttct tttcgatgat tggctgataa ttttgaacga tttgctggaa
     4321 ataaggttct gtataaattg tctggatctc gtaagaattc tcgccatctt ttgacagctg
     4381 gatgatttcc cgaatcagtt tatctaaccg gatcgcatct ttttgcatga tctcaagaaa
     4441 agaagtcaga gtttgcgggt catcttttgc accatccagt aaagtctcgg taaaaccaat
     4501 cagtgaagtc acaggagttt ttaattcgtg agagacgttg ccaacgaaat ccttttgtag
     4561 cttttctaaa cgtcgcactt tcgttaagtc atacgccacc cctagaatct gtccggtacc
     4621 ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt
     4681 gatctcctga tggatcagcg gatgctcagg agtcacttga tgaatcagct gaatgagttt
     4741 tggctcttga atgacttctg tgtaccgctg ttcaggttga tagcttacca cgtttaaatg
     4801 tagctgcatc tttggattaa gcagtagtaa acgagaatca gcatcatcaa tcaggaatac
     4861 gccaatcatc agttcgttca gtaacgtata taattgctct tctgtggtag tatacgcccg
     4921 atacatctta ctcatttgtt cgctcaacgc attgatcgtc tgatacaatt cttcccattg
     4981 atcgggtgtt tgcatgatta cttccgcctg attaggattt tgcaccattt tttttagtac
     5041 gggcagcacg gttttcaatg gctggttttt ttgatagatc agataataga tcattcctgc
     5101 aagaacaagg aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac tctcagttct
     5161 cggtaaaaaa ccgcttgtcg gttctgcgat acgtaaaatg gcttctaact ggccactttt
     5221 tttgactggt aatgcgacat ataaaagatc cccattcaaa gtagtacttt tgcgcaaagc
     5281 gctacctagg ttcccaccat tcaatacggc tttgatttcg ggacgggagt ttcgctgttc
     5341 attcaatgtt tcattgtttg tgtcaaataa gatctttcca gtagcatcca ttaaagtaat
     5401 ccgttcgttc gactgatgga cgaattcttc caaagcagtt ttgttttgcg ctgcttggag
     5461 gttttcgaca tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt agctttcttg
     5521 ctgttccacg atctgctgtt gaaaataatg ggaaatcaat tgccagccac caaaaaacaa
     5581 actaactaaa agaataagaa agaagagacg ttcataaatt tttttccatt tcatcgtttg
     5641 ggttcctgaa acttatagcc aaatcctcta atagtcacca agtactttgg gtgttttgga
     5701 tctcgctcga ttttttctcg caaatgacta acgtgtacat ctacgattcg gctttgtcct
     5761 gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc ggtcgatcac acggtctttt
     5821 cgtttcatga aataaacaag taattcaaat tctttcggtg ttagttcaat cagatggtcg
     5881 tctacggtca cttgataatt ggtcaaatca gcagtgattt gtccaatcga tagatattca
     5941 ggctcagctt catcttttga ttgcttacga ggttcaattc gtctgaatat tgctttcata
     6001 cgggctaata cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc acctatttct
     6061 aggccaatga tccgatccac ttggtcatct ttagccgtca gcatcaaaat cggtgtttca
     6121 tttttttctt gacgcagccg ctgagtgatc tccatgccat ccatcgttgg aagcatcaca
     6181 tctaaaatga tgaaatcaaa tgattgttct aatgctaatt ctaatccttc gcctccatct
     6241 gtggcactgg ttaccttgta gccttctttt tccaaattga acgtcaagag cgttacaata
     6301 gaaggttcat catcaacaac tagtattttt ttcattcaaa tctcctttaa ataggtttca
     6361 ttctgtttat attttagcat agaatcagta caaagggcta ttttacaaaa gaaataaatg
     6421 gtataatagg aaggcacttt gctgaataga aaggaagagc agtcaatgat tggaatcagt
     6481 gcatgtttag gcggtctggc ctgtcgctat gatggacaga aaaaagaagt accagaattg
     6541 atggaactag ttgaaactgg agaagctgtc atggtctgtc cagaagtgat cggcggatta
     6601 ccaattccta gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat
     6661 caagcaaaag tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg
     6721 atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa
     6781 agcccttcat gcggaaccca ttcgatttat gacgggagtt tttccggaat aaaaaaaaat
     6841 ggtgccggag tggctactgc ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa
     6901 gaatggctga aaactagagg tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc
     6961 gctctttgaa ttttattcga ccttattgac agaaaaacaa atgaactaca tggaattata
     7021 ttatgcagat gatttttcat taggtgagat cgcggaagaa tacgaagtaa gccgacaagc
     7081 agtatatgac aatatcaagc ggacaagcaa gatcttggag gattatgaaa aaaaacttca
     7141 tcttttttct aattatattg ttcgagaaca ggtattggaa aatatgactg catatattac
     7201 agataagtac ccggaagata aaaaactact tgaatatgta cagcaaattc aagcaatcga
     7261 agaataaata aaaggaatga agaaaatatg gcatttgaaa gcttaactga acgtctccag
     7321 caggcgatgg gaaaaataag aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg
     7381 atgcgggaaa tccgccttgc cttactagaa gccgatgtta acttgcaagt agtcaaagat
     7441 tttactaagc gcgtacgcga acgtgcggta ggggcagaag tgctggaaag tttatcacct
     7501 gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga tcacacttgg ttctgaaaca
     7561 gcagagttaa ataaatcacc aaaaatacca actgttatca tgatggctgg tcttcaaggg
     7621 gcaggtaaaa cgacctttac tggtaaatta gccaattatt taaagaaaaa tgaaaatgct
     7681 cgtccgctcc taatcgcagg ggacgtatat cgaccagctg cgatcgacca attgaaagta
     7741 ttaggtcaac agctagacgt tcctgtattt gatatgggaa ctgaagtcag cccggtagag
     7801 atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa acgactatgt attgatcgat
     7861 acggcaggac gtctgcatat cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa
     7921 ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca tgactggtca agatgccgtc
     7981 aacgtagctg atagcttcaa ccagcaatta ggtattacag gggtcgtcat cactaaactt
     8041 gatggggata ctcgaggcgg ggcagcacta tcgatccgct cagtgacagg cgcaccaatc
     8101 aaattcatcg gttctggtga aaaattgact gacttagaag tattccaccc agatcgtatg
     8161 gctagccgta tccttggtat gggggatatg ctgacattga ttgaaaaagc gcagcaagac
     8221 tatgacgaga aaaaagcgga agaacttgca cagaaaatgc gggaaaattc attcgatttc
     8281 aatgacttta ttgaacagct tgatcaagtg atgggtatgg gcccactgga agatctaatc
     8341 aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa
     8401 gatgttgaac gcaaaaaagc gatggtttat tctatgacgc cagcagaaag agcaaatcct
     8461 gatctgttga acccaagccg tcgtcgccga attgctgccg gttctggaaa cagtgttgtt
     8521 gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc
     8581 aaaggggata tgaacatccc aggtatggac caaatgtttg gttcaggcgt caaaggaaaa
     8641 ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg
     8701 aaataagatg aaaaaaacag cttcggtaca gactgaaaat gtctactgcc ggagcttttc
     8761 tatcagcaat ttttattctt tactaataca attactctaa acgaagaggt ggcacagatg
     8821 agatgtccgt ggagtgaaca aacagaaagt atgcggcaat atcatgatac tgtctggggt
     8881 gttcctgaat atgatgacca aaagctgttc cgaaaattga tgcttgatat taatcaagcc
     8941 ggattgagct ggcagacgat tttaaataaa agtgaagcat tcgatgaagc atatgatcga
     9001 ttcgatatta aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact aatggaaaac
     9061 aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc
     9121 ttgaaaattc aagaagaaat cggcagtttt tcagagtatc tttggtcgtt ttctaatgga
     9181 aaaatcgttg attcttctta tcattcacaa gaagaagtcc caacaacaag tcctctttca
     9241 gatgaattag caaaagatct aaagaaaaga ggatttaaat ttatcggaag cacaacaatc
     9301 tatgcttttt tagaagctgt cgggatcatc aatgatcacc ttgacagttg tttccgcaaa
     9361 gaagaggtcg tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat catattttat
     9421 ggtgctataa gtttagatgg ctatttggca acgaaaacgg atgatcttca atggcttttt
     9481 gacactccaa caggtaagca aaccacttat gaagcttttt atgaaacaat cgatacgacc
     9541 atcatgggca gaaaaactta tcaggaagca aaaaaatatt tggagaccga agcaatttat
     9601 ccagagaaaa agaatttcgt tttttccacc cacgcacaat tacaattgcc agatgctgag
     9661 gtcgtaaacg aagatcctgt tgcttttcta aaaaatctac aaatgacaga aggaaaagat
     9721 atctgggttg tgggtggcgg tcgattgatc aaaccgctat tggaacatca aatgattgat
     9781 gaatggtata tccagattac ccctgtatta ttaggggacg ggattccttt attccaaaaa
     9841 ggaacatatg aagcgcgttt tgaaatgcta gacacgacac gattcggtga atttatcgaa
     9901 ttgcattatg tacgaaagta gaagtaaata aaataaaaaa ggcaactata tgcagaaatg
     9961 tcctgtacat agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga
    10021 aaaatctaga tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt
    10081 accattttca tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt
    10141 tggcacattc aaaaagacaa gtgattgtcg tagatggtgg tttgcgccaa agccgccgat
    10201 cccacctggt gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata
    10261 aggtcttgag cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc
    10321 aggagtaacg aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc
    10381 agcaggagcc tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt
    10441 tacaaataca ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt
    10501 gttgtaagaa tcttttctta aactaccaat aaaaaatcca attttttttg tcatacgatt
    10561 tttcctcctt caaactatct ataacaatct tacaaaaaat aagtaactgg agcaaataaa
    10621 aacacttact gacaaaagct acatagaaga tagattactt atttttaaca aaaaagaagt
    10681 ctaaaatctt ctatttccta aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct
    10741 ttacagggta aaaaaaaact gttacactac atcttgtgaa taaaacaatg gaggtggaaa
    10801 taaataatgg cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac
    10861 cgtatcgtcg tagctgattc acgttctcct cgtgatggac gtttcatcga aactgtaggt
    10921 acttacaatc ctttgaaaga ccctgcagaa gtagttttaa aagaagactt agttcttgac
    10981 tggttatcaa aaggtgcaca accttctgac actgtacgta acatcctttc aaaagaaggc
    11041 gttatgcaaa aacaccacga agctaaattc tcaaagaaat aaggtgaccg gatatgaaag
    11101 atttaagcga cttagtctta acaatcgttc gtccgctagt cacttatcct gatcaagtca
    11161 agttagacgt cgtagagtcg aaagactttt acgagtacaa tctaacagtg gctcctgaag
    11221 atattggccg tatcattgga aaacaagggc gtgttgctaa agcaatccgc acaatcgttt
    11281 acggtgttcg gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa
    11341 agtaaataaa cgtgctgtac tcgaattttc gagtgcagct tttttgtttt tttattaatt
    11401 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
    11461 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
    11521 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
    11581 ccagtccaga aaaaagtagt accaaaacgg ctaccaccac tgaacaaaca atggctacct
    11641 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
    11701 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
    11761 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
    11821 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
    11881 aaacaatcca agttttgcag ccctttgaga tcgacacaaa tggtcagcaa gtggacttag
    11941 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
    12001 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
    12061 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
    12121 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
    12181 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
    12241 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
    12301 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
    12361 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
    12421 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
    12481 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
    12541 gtgttaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
    12601 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
    12661 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
    12721 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
    12781 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
    12841 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
    12901 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
    12961 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
    13021 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
    13081 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
    13141 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
    13201 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
    13261 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
    13321 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
    13381 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
    13441 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
    13501 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
    13561 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
    13621 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
    13681 aacagcagat gaagccaagt gactttttct aaaatattct ttacagtagg ttgataatat
    13741 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctatgagaaa
    13801 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
    13861 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
    13921 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
    13981 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
    14041 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
    14101 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
    14161 tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa cccttggtat
    14221 tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt ttttctttaa
    14281 ttattggtag gggagaataa tggattagaa taaatctacc tataagtagt agtaaaaaaa
    14341 agagcactat gataatatct ttcttaagag taaccgcttt aataaaaaat ccagaatatt
    14401 ctaacataaa tggagtattc ttagaatcaa aaggagaaag aagatggttt atacttatac
    14461 ggagcaagaa aaaatgaaag tttgagacac attcatgaat gatggaaaag taaaacaagt
    14521 accaagttca acaaaaaaga aaaacatcct attgaaagaa gtcttgaaac gctttgacca
    14581 tggagtgact tatacagaaa cagaggttaa ttcgatactg ctgaacgtat tttctagtgg
    14641 cgattatgtc gaacaaagac gttatctaat cacatttggc tttttcaaaa gaagttcaga
    14701 cggtcgggca tatcagatga tgggaattga aaattagaag ttttaattta tactcattga
    14761 aaaaagttca agtatgcgat attggttcta caccgcatac ttgaactttt tttgtcatct
    14821 gtatctataa tacaaagcca agaacgttat tcaaaatatg gatagctatg ataaaagcta
    14881 atgagcgatt cgcagttaaa taagcaatcg tcagtaaaat cgttccagga gcgtacatga
    14941 aaaaatcaat tggatgcgtt acatgaataa agataattct cttttatttg tacgaataaa
    15001 taccaaaacg tacctcctaa aattagattc aaagaagata gcaaagagga aaaagatagt
    15061 tctgcagcag ctatagcggc atgtggattg ctagaacttt ccaaacaact gcctgtttca
    15121 gatcctcgtc atttcgatta cgaagagata gcaatcaaaa tcttggaaga attacagaaa
    15181 aactatacaa cgaaacgagt taacggatca aacggtctgc ttctccatgc cgtttatgat
    15241 aaaaacagct taaaaggtgt agatgaatgt gtgatctggg gcgattactt ttatgtagaa
    15301 ggaatcactc gtctagctaa aacatggtac tgttactggt gatgtataag agaagaggag
    15361 gattcatata atgaagcgat ggaaaaaaat gatgattgga tcaatagttt taggaatgtc
    15421 actcgtattt ggtgcttgtg gtaatcaaga agacagtagt gaagaatcag caaatgaaat
    15481 taccgtttcg acatggaatt atgaaacaac acctgaattc aaagcgctct ttgaagcatt
    15541 cgaaaagaaa acgggaatca aagtgaaagc agtagatatt gcttcagatg attatgatac
    15601 gaaactcacc acgatgcttt cttctggaga cacgacagat gtattgacga tgaaaaatct
    15661 tttgtcgtat tcaaattatg ctttacgaga tcagttagtc gatcaaacag aacgaattag
    15721 agaattagac actgatgctg ctgaagaaac atatgaaatg tatgatatcg atggaaaaac
    15781 atatgcattg ccatatcgaa cggacttttg ggtactatat tacaataaga aaatgtttga
    15841 tgatgcagga atcgaatatc caaaaaatct aacttgggac gaatatgaag atttagcaaa
    15901 aaaacttagt aaaaatgacg gacaagttta cggagcatat caacatatct ggcgttcgac
    15961 aatccaagct attgcagctg ctcaaaacga ggcgaatctt gttgaaccag attatcaata
    16021 tatgactgat tattatgatc gagcattgag gatgcaaaaa gaaggcgcgc agatggattt
    16081 tggcacggct aaatcaacga aagtaaccta tcagtctcaa tttgaagaac aaaaagcagc
    16141 aatgatgtac atgggaactt ggtatatggc tggcatactc gcaaacaaag aagcaagtaa
    16201 gacagaagta gaatggggaa ttacagctat tcctcagaag aaaaaaggag aatcggtcac
    16261 gacgtttggc tcaccaacag catttgctgt caataaaaac tcaaagaaac aaaaagcggc
    16321 gcaagaattt attgaatttg cagccagtga agaaggagca aaagtattag caggagtagg
    16381 agtagttcct tcatatcgta cggatgagat tgatcagctt tattttaatc tcgctggaat
    16441 gccaacggat gaaatttcta aaaaagcttt ttctcctgat gaaatcaaac ttgaatttcc
    16501 gattgatacg tatgggcctg caatcgataa gattttacaa gaagaatatg acttagtttt
    16561 ggttggagat gaaacgccgg aaaaaggaac ggtaaatatg gagaaacgtg tagctgcgga
    16621 aaaagaataa tcatgaaagg agagaagcca agagataaaa cactcttggt ttcgtcatcg
    16681 aaatggagaa tagtataaat aagaaagcta caaatcattt gcgtagaaaa cgaacgtgga
    16741 tcgcttattc gtttatcgcc ccgaatttca ttggtttttt cgtttttaca ttaataccaa
    16801 tcctgttttc gcttgtacta gcatttatga aatgggattc tttttcaacg ccagaatttg
    16861 ctggactaga taactttcgt aaaatggcca acgatgagac attttggatt tctttgaaaa
    16921 atactttttt atataccgct gctgtagtgc ctggcacact tgtttgttcg ttagggctag
    16981 ctttactact aaatcagaaa attagaggga tgaagctttt tcgtacgata tttttctttc
    17041 cgtatgttac ttccttagta gcaattgctg ttgtttggaa tatgttgttt catccaacag
    17101 tgggacctat taatatcttt ttaagacgat ttattgaaca tcctccaggt tggacttcaa
    17161 gttcaaattg ggcgctttgg gcaattgtaa ttgtcagtat ttggcgaggg atgggttatt
    17221 acatgattct ataccttgca ggactacaaa gtatccaaaa agaactatat gaagcgtctt
    17281 cgctagatgg agcaaaccgt tggaaacaat ttttgaatgt gacattacct tcgttgagac
    17341 caacaacctt ctttgtaacg atcatgcttg tcatcaattg tttcaaggtg tttgaccttg
    17401 tgcaagtgat gacggatggc ggccctggtc gagcgacaaa tgtgctggtt taccaaatct
    17461 acaatgaagc attcaaaaaa tttgattttg gttatgcttc agctatttca cttgttttgt
    17521 tcgtaattgt tttgggcgtt acggttgttc aattttcttg gaataagcgt cgtacttagg
    17581 aggtgtgata tggaagagag tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa
    17641 aacatgccct ttattcaatg gttgggtatg attctgctga ttgtattagc tttattgacg
    17701 atcattcctt ttttatggat ggtttctgct tcattcaaag caaataatga agtgttaacg
    17761 attccgattc aatggatccc taaaacttgg catttagaga attatctggt tatttggtct
    17821 cgtattcctt tagtgacatt ttttaagaat acagcttttt taagcgttgt gatcacgttg
    17881 attcagttgt ttacttctag ttttgctgtt tacgggtttt ctgaactaaa ttttaaaggg
    17941 cgcgatattt tatttttagc atatatcggc acgattgctg tcccttggca gtcttatatg
    18001 atcccgcagt tcattatgat gagaaaagtg ggtttaacag atacgttgtg gtcgttggtg
    18061 ctattacaag cgttcaatgc ttttggtgtg ttcttgttga agcaatatta cagtagtatc
    18121 ccagattctt tatgtgagag tgcgcggatc gatggcttat cagagtggga aatctatcgc
    18181 aagatcattt tacctcttac aaaaccggcg attgccagtt tgacgatcat cacattcgtt
    18241 aatacatgga acgattatat ggggccattc atttatttgt cttctacgga aaataaaaca
    18301 atccagttag ggttgaaaat gtttattggg ctatttgatg cagagtacgc attaatcatg
    18361 gctgcctcag ttgtttctgt tcttcctgtt gcttttatat ttttagcgat gcaaaaatat
    18421 tttgtagaag ggatcgcttc atcaggaatg aaggaatagg aggtgattat gaaagagcag
    18481 cgttttgaca aaaatgttta tatggaattc ttttattggt tgtatcaatc attggcaagt
    18541 agcctttgtt tttggtgtgt caatatcttg tatatagcag ctgcgttcct tttgtcgata
    18601 gatggaagga atgtcttttc ctttattttt tctttacttt ttattggtcc aggttgcatc
    18661 gtgatgatag gtatgacaga agaattagca aaagaaaaaa gtttttcacc agtacgtttg
    18721 ttcataaaga cgtttcgcca attttggcta aagggatttt tttattggct atttgcttgg
    18781 atagttagtg tgataatgat ttttgattgt ttctttttta tacgtttttc ttatggaaaa
    18841 tggttgattc cgttgtttgt tttacttgct tgttttactt gcttgtttat cagtttcctt
    18901 tagcatcaac tgctggtatt ttcaggtgag aaatccagcg tcgaaaccaa atcaagttct
    18961 acggatagcg ttctactata cgttgaaaaa atggtatgtg agtttactag attttttact
    19021 attgacaagt ttgtttctct tctttttcgt aaagccccaa tggtgtattc tactaggtcc
    19081 ttctatcgta tttggtttga tttacttcaa caatcgtaaa ttgatgagaa ctatggatct
    19141 gtagacttta taaattgaaa ctttcaatta agaatgacag ttttttatgt aaaaactgtc
    19201 attctttttc attttttgaa acatatggct aaacaaatga aataaaacat gctatacttt
    19261 tgaatgaaac aggtttagaa actgtatcac ttatttgtga ttatttctaa ctaataatta
    19321 aaaataaagc ataaaaagga cgatgttcaa tggctgaaaa aatgaaacaa ggttcgaaat
    19381 ctgtagtaga aagtttgatt aatcatcatg ttgattatgt attcggtata ccaggtgcaa
    19441 aaatcgatgg tgtatttaat gaattagagg atcaaggtcc tgaattaatc gtgactcgtc
    19501 acgaacaaaa tgctgcattt atggcacaag caatcggacg tattacagga gagccaggag
    19561 tagttatcgc aacaagtggt cctggtgcta gtaacttagc tacaggtttg gtaacagcga
    19621 cagcagaagg tgacccagtt ttagcaattg ccggacaagt aaaacgaagc gatctattga
    19681 agttgaccca tcaaagcatg gataatgcag cactgttcca gccaatcaca aaatacagtg
    19741 cagaaatcca agatccagaa acaatttcag aagtgattgc caatgcttat cgtatggcta
    19801 agagttcaaa aaaaggcgcg agctttatca gtatcccgca agatgtagtg gatgcacctg
    19861 tccaaggtaa tgtcatcaag ccattgagcg atcctaaatt aggttctgct tctgcagatg
    19921 atatccgtta cctagctgaa cgcattagag aagcaaaact accggtacta ttagtaggta
    19981 tgcgaggatc aagtgaaaaa gaaacgttag ccatcaggca attagttgaa aagacagcat
    20041 tgcctgttgt agagacattc caagcagctg gagtgatctc acgtgaatta gaagcgcatt
    20101 tctttggtcg tgtgggttta tttagaaacc aaccaggaga tatgcttttg aaacgaagtg
    20161 atctagttat tgcaattggc tatgatccaa tcgaatatga agcaagaaac tggaatgcgg
    20221 aaaaagatgc gcggatcatt gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc
    20281 agcctgaaag agaattgatc ggtgatatct cagccactct tgatttattg acaggttcat
    20341 tagagcctca acaagtgtca gaagatgcaa aagaatattt agcatctctt caagcaaaat
    20401 taacggagcg ggacattgtt caatcaaaag gagaagcagg tatcttgcat cctttagaag
    20461 ttattaacac gcttcagtca aaagtaacag atgatatgac tgttaccgta gatgtaggta
    20521 gtcattatat ttggatggca cgccacttta gaagttatga acctcgtcat ttgttattca
    20581 gtaatggtat gcaaacgctt ggtgttgcat tgccatgggc gatttctgct gcattggtgc
    20641 gtccaaatac acagatcgta tcagtttccg gcgatggggg atttttattc tctgcccagg
    20701 atttagaaac ggcggtacgt aaaaaattga atatcgttca tttgatttgg aatgatggac
    20761 actacaatat ggtagagttc caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact
    20821 ttggtccagt tgattttgtg aaatatgcag aagcttttgg cgctaaagga atccgagcta
    20881 caagtgtaga ggaattagaa aaagctttag aagaaggatt tgcgacagaa ggacccgtaa
    20941 ttattgatat tccgattgat taccgtgata atgagaaatt gggagaaaca atcttaccag
    21001 atcaattcta ttaaggaagt gtcgacatga ctgaaaaaat cttataccag cacgggacat
    21061 taggagcttt gatggccggc ttgatggatg gaacagaaac gattgctcat atattagaaa
    21121 aaggaactct gggattggga acattgcatg ggcttgatgg tgaagtcatc tttttagatg
    21181 gcgtagctta tcaaggaaga tcagatggct cagttgccca attagatgga agtgaactta
    21241 ccccctatgc agcaattaca gattttacgc cagatactag tttttctgtc tcaaaaactg
    21301 cagatggaga acaattgaaa aaagacatat tagtaagaga agccggggaa aacctatttt
    21361 tggcagtgaa gataactgga ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag
    21421 aaaaacctta cagaagacta gcaaaaattt cagagtctca accagaattc caacaaagca
    21481 atgtccaagg aacattagtc gggttcttta cgcctgaatt attccaagga gttgctgcag
    21541 cgggatttca cctgcatttt atcgatgata cacatacatt tggcggacat gtaatggatt
    21601 ttgaagtggc tgaaggaaaa gttgagatta gccgcatctc gtcccttgta cagcattttc
    21661 cagttgatga tccgactttt gttcacgaaa cgattgatta tgctgattta gcagcagaaa
    21721 tccaacaagc agaatagaaa aagacagcga gatgcgttca aggtgattaa cctgacgtat
    21781 ctcgtttttt tgaggagaaa acctagcgtt tacaaaaaaa ctgagctata atcttctcca
    21841 tagatcaaaa aggagcggtt tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg
    21901 aaattttatt attacagcaa caaggattat tagaagcaga agataactgg ttagcaaatt
    21961 tagcaaattc atctgcattg ttgaatgaga ctttgcctga gactgtcttt gcaggatatt
    22021 atttattcga tggtaatgaa ttgattttag gaccgttcca agggcgagtt tcttgcacac
    22081 gtatcaagat gggaaaagga gtttgtggag aatcagctga aaaacaagaa acattgatcg
    22141 ttgatgatgt caaaacccat gcaaattaca tttcctgcga ttcagccgca caatcagaaa
    22201 tcgtagtgcc aatggtaaaa gaaggtaatt taatcggtgt tttagatatc gattgcggag
    22261 taacgaaagg gtatgatgaa atcgatcaag catttctaga aaaatatgta gatcttttat
    22321 tagcaaattt taatataaat gctttttcat ttattaataa ctaaaatcgt ctattgaaac
    22381 aaaaaagaag agaaatcaag aataaaaaga gccggaaaga cagtgggcat ttctgttttt
    22441 cggttttttt atttttttat aaaatcacaa gtgttattga ttttcataat catattttaa
    22501 tgttattatc attttaaata atgaaaaaac attaaggatg gatgaaatat gaataacgaa
    22561 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtgatt
    22621 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
    22681 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
    22741 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
    22801 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
    22861 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
    22921 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
    22981 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
    23041 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
    23101 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
    23161 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
    23221 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
    23281 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
    23341 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
    23401 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
    23461 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
    23521 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
    23581 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
    23641 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat agcttttgga
    23701 tttttacagt aattttatct taataatagc aatattatta cttctaaata tttggttttt
    23761 tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt cttctgaaaa
    23821 aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca gtttactttc
    23881 ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta tatctattat
    23941 tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag agaagtcaaa
    24001 aaataattaa agcaattatg ccttttagta caggaattat tacttcttac aaatgtatag
    24061 cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa caattatttt
    24121 atgttaggat gaattaattt ctctagagtt tata
//
LOCUS       DAZKCN010000040        23908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000040 DAZKCN010000000
VERSION     DAZKCN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.041"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..3301
                     /locus_tag="ACG8K3_001415"
     CDS             23..3301
                     /locus_tag="ACG8K3_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP9145299.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3842..5749)
                     /locus_tag="ACG8K3_001416"
     CDS             complement(3842..5749)
                     /locus_tag="ACG8K3_001416"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP9145300.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5879..6754
                     /locus_tag="ACG8K3_001417"
     CDS             5879..6754
                     /locus_tag="ACG8K3_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP9145301.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     ALIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6834..7808
                     /locus_tag="ACG8K3_001418"
     CDS             6834..7808
                     /locus_tag="ACG8K3_001418"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9145302.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7988..9133
                     /locus_tag="ACG8K3_001419"
     CDS             7988..9133
                     /locus_tag="ACG8K3_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9145303.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            9270..9614
                     /locus_tag="ACG8K3_001420"
     CDS             9270..9614
                     /locus_tag="ACG8K3_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP9145304.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9885..11155
                     /gene="efmA"
                     /locus_tag="ACG8K3_001421"
                     /pseudo
     CDS             9885..11155
                     /gene="efmA"
                     /locus_tag="ACG8K3_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(11308..11907)
                     /locus_tag="ACG8K3_001422"
     CDS             complement(11308..11907)
                     /locus_tag="ACG8K3_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9145305.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12311..13432
                     /gene="mnmA"
                     /locus_tag="ACG8K3_001423"
     CDS             12311..13432
                     /gene="mnmA"
                     /locus_tag="ACG8K3_001423"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9145306.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            13678..15099
                     /gene="arcD"
                     /locus_tag="ACG8K3_001424"
     CDS             13678..15099
                     /gene="arcD"
                     /locus_tag="ACG8K3_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP9145307.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15113..16231
                     /locus_tag="ACG8K3_001425"
     CDS             15113..16231
                     /locus_tag="ACG8K3_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9145308.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16291..17583)
                     /locus_tag="ACG8K3_001426"
     CDS             complement(16291..17583)
                     /locus_tag="ACG8K3_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9145309.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            17733..18293
                     /locus_tag="ACG8K3_001427"
     CDS             17733..18293
                     /locus_tag="ACG8K3_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9145310.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYLSLRFLG
                     YFTKESTIDPEDSYFWMINDFIATLP"
     gene            18422..18799
                     /locus_tag="ACG8K3_001428"
     CDS             18422..18799
                     /locus_tag="ACG8K3_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP9145311.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18732..20804
                     /locus_tag="ACG8K3_001429"
     CDS             18732..20804
                     /locus_tag="ACG8K3_001429"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9145312.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21015..21962)
                     /locus_tag="ACG8K3_001430"
     CDS             complement(21015..21962)
                     /locus_tag="ACG8K3_001430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9145313.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22207..22671
                     /locus_tag="ACG8K3_001431"
     CDS             22207..22671
                     /locus_tag="ACG8K3_001431"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9145314.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22733..23776)
                     /locus_tag="ACG8K3_001432"
     CDS             complement(22733..23776)
                     /locus_tag="ACG8K3_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP9145315.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     RATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7766 a         4386 c         5047 g         6709 t
ORIGIN      
        1 atttgttacg ggaggaatac acttgttaaa aaaacgtata aaatacagtg cgctaatgat
       61 gaatctcctt atgctcagta ccccggtttt aatttctgtc gttgaagttg ctgctaacga
      121 acaacagtct gtgaacgaag aaaatgcaca cgtgaacatt gaagggatgt caacaatgga
      181 ggaaatacca agaactagtg aagatatgga tacggaagaa ggaaatgatc ctatgatgaa
      241 agaatcagaa aatgaaaaga caaaaaagca atctgaagaa gcaaatgaag agtggaaagg
      301 ggttgttttc ggagaaagca caagtgctag taggtatgcc attgaaccat tagaagacgg
      361 cgtgcggctc tcttccacga ataacggcgg aaaattccag tcaagcggat cagatggaat
      421 gacctattat tatcaagcaa tccccaaaga catcaacttt aggatgcggg cgacgatcga
      481 aatagaatct tggacctata cgaatgccca agaaggattt gctttaatgg taagagacgc
      541 agtaccaaaa aatcatttgt ttgggcaggc attttattca aattcatttg ccatactagg
      601 cagtcggatc gaatatgtct gggactcgga tagacaagaa gtggtcaaca cgagcggcag
      661 caaatatacg atgcgacttg gattggggac acgggcaatc acaggaatat catctgaaga
      721 tccgcaagct gcgccagctc ctgggagtgt ctcagtggaa acaacacctt tagagacttc
      781 agcagggttc ttggaaaaag aaacaggttc ttataatata ttcggaaacg gtcatgggaa
      841 tctgtttccg gccacgaact cgattactaa actggacgta gagatgaaaa aaacgaatac
      901 tggtttagag gcatattatt acgatcctga aacaggagaa gaaatggctt ctgacatcat
      961 gtatgactgg gaaaaattat acgcttcaga tgaatcggtt atttatgcag gatttgctgc
     1021 agcaaggaat atgacgatca aggtagaagg aatcgaagtt gcttactcag atcctgcgac
     1081 agatcctcct gggcaagaaa gacctactcg cttgattgat ccgatttaca ctgtgacctc
     1141 tagtaatcat tcaggaaacc aagatcatac tatgtccttt agagcaaatg ctgacggatt
     1201 attgacaatt aaaaataaag caacaggaga ggtgcttaag aacgcaaaaa acttggctat
     1261 cactgcgaat cgggaatttg attatcagac aaaactgatg gaaggaacga acgaatttac
     1321 tttcagtttt acgccagata aaaactatcc gccagaagaa tacgtagaga tgactagtta
     1381 tgaaacaaaa gagattcttc atacagtgga ctaccgtaaa cctaaatatt cgacagttta
     1441 tgtaacacca gaagggtcag atgcaggaaa cggaagcaga caaaatccat tatctctgac
     1501 acaagcattg agatacgcat atgctgggca gacaatcgtc atggcgcctg gaacttattc
     1561 cttcgaaaga ggattgacga ttccaaaagg cgttaacggg acaagagaaa acccgattca
     1621 gttgattgca gagaaaaatc cagaaaacaa gcgtcctgta cttgatttta aaggaactgg
     1681 aaatgggatg acactgttca gtcactactg gggtttacga gggtttgaca ttacaaatag
     1741 tgcagctatg caaaaaggat tacagatttc tggaaatcat aatttgatcg aagagattca
     1801 tgcttatcgg aatggaaata caggaatcca aatcagcggt agctcaaatg atcctttcga
     1861 agcatggccc gcgcataact tagtcaagaa ctgtacatct tttgaaaatg ccgatccagg
     1921 atttgaagat gccgatggat ttgcggccaa actgacaata ggagaaggaa atgtatttga
     1981 cggttgtatc gcgtatcata atgcagatga cggttgggac ctttttgcta aaaatgaaac
     2041 aggttcgatc ggaaaagtaa tcatcaaaaa ctcagttgct tatcgaaacg gctgggtacc
     2101 aggaattgag ggagaaggta acggtaatgg attcaaaatg ggcggttctt ctttaacggg
     2161 ccctcatgaa ttgatcaata gtttgtcttt cgaaaatctt gcgaaaggaa tcgattccaa
     2221 tagcggaaca gatattatcg tcaatagcag tacaagtttt aataacggtt catataatgt
     2281 ggctctttat acctcttcag caggaaacac agactaccat gcttctggta tcttatcttt
     2341 ccgaacagag aatcttgaga tgagagagca gatccgccca ttgaatcaaa acgaagatca
     2401 aatctaccac tcgttgaact actattggaa tgaacgagag aaacaatcag aaaatactct
     2461 tggacatacc atatcaaaag attggatcga gtctctaaat acatgttcga aagaaggtca
     2521 gcagcctgtt acgcgctatg aaaatggaag tatccatgtt catggactga tgcagattaa
     2581 accggggaat cgtcaaacag aaaaggaaag agtggatggt cttccattat ctgtaggtgc
     2641 tatttttgac ggagaaacct caccgtcttc ggaatatcca gagtatgcaa tcgtacaaga
     2701 accaaatcag acaaaaccaa atgaacaaaa taatccgatt attatacaag aagcaaatgg
     2761 tgtggcggag aatgcagaag gaaatatcat tgaactgaag ccttttacag tttttgtgtc
     2821 aactttcaca ccaaatcaag gaacgcctgt ctttgaagat atgcgttatt tcccagaaaa
     2881 gatccaaatc gttgaaacac agtcaagtgc tcaattttct aatcataccg ctattttttc
     2941 agcaccttca aaaggagaat atacgctaga agtcacttat cgttcggaaa tatttgatgg
     3001 ggaaaaatgg tctgctcata cagaatctgt tacccagcaa aaagcgatca ctgtgcgaga
     3061 aactgccgat gtgcccccgg gaaatgacaa accagaaaga gaaaatccag gaaatgaatc
     3121 accaaatgga aaaccaaatg gaaaaccttc tcatccggat aggacagcaa aagaaagtga
     3181 aaaaaatggc actcaaggac tcccaaaaac gggagaattt aataacccgt tgctagcatt
     3241 agctggagga attttgttaa tcggtgtagt tgtttatgtc atgaaacagc gaaaaaaata
     3301 agtggaattt ctccttttat gatgtaaaac aattgactga ggaaagtaaa aatagaagga
     3361 aatgagacca atagatgtct cacttccttc tatcttttta tagatgaggt tctccttgct
     3421 gcttttgatt ggctgttctt attacttgat gcaggacagc tttttcacaa tctctccact
     3481 aaacggtgtt caatcagctg accctcggta ttagctcaac aaacggcaaa atatgaggaa
     3541 ctatttttcc gtttttttct ctaatactca ggtcaggacg ctgatttcac aacctctcga
     3601 taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa
     3661 agccattttc acaaaatttt tcttactact tgaaactaag cagctttttc acaacctcta
     3721 atacatggta ttccagaagt aacttcttcg aaaaataagc cgaaatgtca caaaaatttg
     3781 agaagcgatt ttcccaaatt ccttcttatt tcttgaagct gaccacttct ggcacaacct
     3841 cttaccagat tcgcactctt ttttctggcg ctagatacat agcgtcttgt tccgtgatac
     3901 caaatgtttc atagaattcg tcaaggtttt ttggttgcac gtttgctcgg agtttagcag
     3961 gggcatggac atcgatttgc agtaaaagtt gttggtattc tttttttgct tttgtacgcc
     4021 aaatcgttgc ccaattgaca aagaattcct cgatagaaag atctgattcc gtttttgctg
     4081 cttccaatgc acagcttaga ccgcctgcat cagcaatatt ttccgaaacg gttaattttc
     4141 cgtttacttt tccgtcagca aaaggcagac catcgaattc ttcgatcatt gcttgtgctt
     4201 tgttttgaaa ttgtgtaaga tcttcttctg tccaccagtt gttcaagttg ccatattcgt
     4261 caaataaagc gccgttatta tcaaatgcat gggaaatctc atgagcgata acagctccga
     4321 ttccaccata attagcagag cttgattgtt ctagactgta aaatggtgct tgaaggatcg
     4381 ctgccggaaa gacgatcaca ttccgaaatg gatgataata agcattgacc gtgtcagcac
     4441 tcatttccca ttctgaacga tccactggtt tgttccattt gctgaaacga tcttttaatg
     4501 tgagtttgct gaaaacaaga gtatttgaaa gcaaagtacc accctgatct gcaggtactg
     4561 ttttgaactg gtcgaataat gctgggatct tatcggggta gcctacttgg atacctaagt
     4621 tatttaattt tatgacggct ttttcttttg ttgcatcccc aagccagtca ttttcttcta
     4681 gacgtttttt ataaacggcg atcatttttt tcaccatttg ttctacgtct tttttagcag
     4741 cttcgccaaa atattttttt ccgtagtaat cgccaactac ttgatcgaat tgtccagagg
     4801 caaggtaata cgctgctttt ttctgaggca ttgcttcgtc tgttccagac aatacgcgag
     4861 aatagattcc gctgacttgg cggaattctt ctgatagata accactaaga gaacgtactg
     4921 ttttaatgat catccagctt ttgaataacg gaaaatgagc ttctgtcagg atgtcattca
     4981 aatgatcgaa gtacactgga tcagttacga tcactttatc tggagttgtc ccaatcaaac
     5041 ccgtgacaag ttttttcaag tccagtttat ctgtgtattt aataaatgta tcaaaagatt
     5101 gcggattata cattttgctg taatctgcgt tttcttctgc actttttaca tgaggagcaa
     5161 tcaatttatc aaattgcact gcttgttccg tgattgcttc tgcctgactt ttttctttgc
     5221 cggcaagctc taataactga acagccatat caaagaaaac gcctattagt tgtgcaccgt
     5281 tgggatggtc tgatgcataa tacgtcttat ctggaaggaa taaagaaggc ggataagcaa
     5341 agaaggcgtt cgtttgcgca ttcttcatat ctgcatctac atccagatta aatggcaatg
     5401 gtaatgtatc caacagccat tctggaaatt gctcattcaa ttcgtcgaaa gactgcaatg
     5461 attcaacttt ttcgattata ggttgaagtg gttgagcatg caactcattt cttcggtcat
     5521 aatcgtttgc cagttgataa tatgcaagga aatgtttcat tttttctgaa gacacttttt
     5581 ctggatgggc aagcagatcg tctgtttctc ccatcaaaag ttggtcgatc ccgtcaacta
     5641 aatcttggaa accgcctgta gctggtttat ctgcagggat ttttgctgtt ttttgccatt
     5701 cttcattgac tgcttcatag aaatcttgtt tcaatcgttc tttgttcatt catcgatcgc
     5761 tccttttttg tttcgtgtgc ccattatctt acaaaatgaa ggaattaaca aatagaacat
     5821 aaaaaattga aaattcgaac aatcaaactg atttgttttt ttcaatcctt cacttataat
     5881 gaaaaaaagg aggcgatccc aactgaatca agtagtggat aaacctttat atttcaaagt
     5941 agataaaaaa attaaaaaat tagccagcac tcagcagctg cagtcaagaa aaagtaaatt
     6001 gcttttttta gctctggttt ttgaagatca aagttatgtg attatcgatc aatcaggaca
     6061 tccggtagaa tattctccag caaaatatac ttatcaagag ggtctttctt gtaaaaaatg
     6121 gaagctgatc aatgaagagc caatcgagtt atctagatgg atcaacagaa aagaagatat
     6181 tcccgctttg atcgaagaaa aaaggtcagg aaaggaactc gcaaattgct gggtgggatt
     6241 gccagaagaa cgttttttga ggtataagaa atgggctacc ccctcaggct atctttgtgg
     6301 tacttacgcg gcagcagttc tccttgccta ttatcaagac tatcgtaagg agtggatgct
     6361 tccaaatgag attcgcaaga aaaatacggc tgattcgttg gttttgacga aggcattgcg
     6421 tagccagatc caaccactag gacttcctac gattcctttt caggttagta cagggatttc
     6481 tagtttttta aagaagaatg gcaatcatga gcgtgcacga gcaactcttc taggttcatg
     6541 gcagcgagct acaaaacgta tacgtgaagg caaacctgtg atgattggta ttttgaaagt
     6601 acttggcagt acatatggca atcattgggt gacagcgtac gcttattttg aaactgaaac
     6661 aggagaacgt tattacaaag tacacgacaa ctggggcgat tatcataaag tcattcctgc
     6721 cagttggagt aacggaacag tttcacttcc ttaagaattt gctgaaatta acagaaaatc
     6781 ttggaaaaat atgttaaact atcgaagaat agagaagttt tggggaggac tacatgagta
     6841 ataagtatca ggatgacacg ttgaagattt ttagtttgaa tggcaaccgt ccattagctg
     6901 aaaaaatcgc gaaagtgttc ggaacagaat taggtaaaag cgtcgtgaag caatttagtg
     6961 atggcgagat ttccatcaat atcgaagaaa gtattcgagg ggatcatgtg tatatcgttc
     7021 aatcaacgaa tgcacctgtc aatgactact atatggaact actgatcatg atcgatgcaa
     7081 tgaagcgagc aagtgcgaaa acgatcaatg tcgtattgcc ttactatggg tatgctaggc
     7141 aagaccgcac ggcgaaacct catgaaccga tcactgcgaa attgatcgct aacttgatcg
     7201 aagaagcagg ggcaactcga gtactgactt tagatctgca cacggttcaa gtacaaggat
     7261 tctttgatat tcctgtagat aatttattta cgatgccgtt gtttgctcat tattaccgtc
     7321 aattaggtct gacgggagat gacatcgtag ttgtttcgcc aaaaaacagc ggtgttcagc
     7381 gtgcacgtag tctttcagaa tatttgaaca gcacattagc catcgtagat catgcagatg
     7441 atgcttcaac tgagggtggt tatgtgatcg gtgatgttca aggaaagaca tgtatcatgg
     7501 tagatgatat cttgaacaca ggagcaacgc ttgcacgagc agcaaatgta ttaaaagaaa
     7561 acggcgcaaa agaagtatat gcctgtgcgt cacatggatt attgtccgat catgcgaaaa
     7621 gtattttaga caatgcacca atcaaagata tttgtatcac tgattctgtt ttcacagaag
     7681 aaaatcgtca tccagaaaac ttaacaatcg ttacatgttc agaattaatg ggagaagcgg
     7741 tcaaacgcat ccatgaaaat acaccgatga gtccgttatt ccgtctggaa gaaaaagaat
     7801 tcgaataaac aaaaaaaaga gggaaagaag ttgtgacaca agtgagcagc ttcaaggagc
     7861 tgcgacaaga cttttgtcat agctcctttt ttctatgccc atgaaacgtc ctctagacaa
     7921 aaatgagctc ttctttggtt tgttcgctat tttatgctag aatgtgccta gcaataattt
     7981 ggaagggatg aatgaagtga aaactattta tctggatcat gcggcaacta cccccatgca
     8041 tccacaagta attgaagcga tgacggcaat catgcaagac acgtttggaa atccttcaag
     8101 catacatgga tttggtcgcc acgcgcatga aaaattggaa acagcaagac aaatcattgc
     8161 agacagtctg caagcaaaac ctcacgagat catctttaac agtgggggaa cagaagggga
     8221 taatacagct atcttagaaa cagcgttctc gtgtcaaaaa gaaggcagac atctaatcac
     8281 tacagctatc gaacatcctg ctgttcttaa taccatgaaa tatttagaga caaaggggtt
     8341 tgacgttaca tatctgcctg tagatgaaaa agggaatctg tcgatcgaag aggtcaaaaa
     8401 agcgttacga gaagatacga tcttggtctc tgtgatgtat gggaacaatg agatcggaaa
     8461 tttgatgcca atcaaagaaa tcggtgaact tttagcggat catccagctt atttccatac
     8521 agatgcggta caagcgtacg gaaaccaaac gatccatcca gaagaattag gaatcgattt
     8581 attaagtatc tctgctcata aaatcaatgg gccaaaaggc gtcggtttct tatataaacg
     8641 agacggagta aatatccctg cattgctttt aggcggggag caggaagaaa agcggcgtgc
     8701 aggtacagaa aatttagcag ggatctgggg tatggcaaaa gcagttgaac tgctgtcacc
     8761 tgaagaacgt gaagcacgta acaacaaata taaaggattc cagcaattga ttcttgatac
     8821 attagaagca aacgaaatcc cttatcaagt aaatggtgat ttaacgaata aactccctca
     8881 tgtgttgaat cttcgcttaa ttggcgaagc aaatgatttg ctactgatga agcttgatct
     8941 taatggggtc gctatttcta ctggttcagc ttgtacagca ggtaatatcg agccttcaca
     9001 cgtattagaa gcaatgtatg gagaagatac accgatacta aaagaatcca tccgtatcag
     9061 ctttggtttg ggaaataccg aagacgacat ccagtatttc cttgaacaat tagttcgtgc
     9121 tgtcaaaaaa taactagtga agtcattctt atatgaacag atgtctgaga gaacgccttc
     9181 attttttagg gaacttaact gaaaaaccta gtaaaaatca ggataaactt ttataataaa
     9241 gagaaactaa ttttatcgag gtgaaaatca tggcatttga aacaacagca acggtactgg
     9301 gcgcaccaat tagctatcgt ttgcatcctg atgcaaaaag atatacattg cgcgataatg
     9361 gatttacaga aacaaacgga ggcaattatc aattgatccg accattagat gccacgccgc
     9421 aaagcaaaga aggattcaaa ttaaaaatca ctgtatcaaa agatattcaa acattgaaaa
     9481 tgtcgatcac tacagcaaat ggattgaaag cagtcaatat tttcaaagat ccaaaacaga
     9541 aaatgagcca agataaattt tattttctga tggatgggat gattagccgt gggttattcg
     9601 aaaaagtcga atgatctgtg gatataaaaa gacaactagt tttgaaacgg cctattgtta
     9661 gatgactaac tttgtaagga caacgcaaat tagttgtctt ttttattttt aaatttaaca
     9721 catgttgtta tttttaatat tttgctgatt tattgtttct aagtaaagaa atttaaattg
     9781 aaacgattgt tatttaggga aaaaggtatt ttttgaaaaa agaatcccgt ataatgattg
     9841 ttgtgcaaac gctatacaaa atgaaaaaga aaagagggat gtttatggaa aatgaacagt
     9901 cggtcgtttt aacgaattgg aagagaaatt atttgttctt tttatcggga cagtttttgt
     9961 caggaatcac tagtatggtc gtccaatatg cgatcatctg gtatttgaca agagaaaccg
    10021 gttcggcgac aattttgagt tttgctactt tactaggaat gattcccatg gttttactaa
    10081 gcccatttgt agaaccgcta gtggatcgct gggataaaaa agctcttttg attgtcacgg
    10141 atattattgt agcgattttc gcgcttattt tagcagtagt cggaacgatt tcagaatcct
    10201 tcccgatttg gctagttttt gtgtctttgt ttatgcgttc ggtcgcacaa acattccaaa
    10261 tgccaacgat cagtcgatta tgccgacaat cgttccatct tctcacataa caaggacgaa
    10321 tggacagtta ggaatggtcc aatcagcgaa tttcatcatt gcgccagctc taggtgcggc
    10381 tttgttttca gtcgttcctg tcaattacct tattttatta gatgtattgg gggcagtttt
    10441 tggtgtcggc ttattgattt ttgtgaagat cccaaaagtt tctcctgaaa tattggaagt
    10501 tccgcttact atttttaaag atgcaaaatt tgggctgcag caattgatgg ataacaaagg
    10561 gttatggtac ataacgatca atggtgcatt tgtgatgctg ttgttcatgc cagcaatcag
    10621 tctgtatcca ttgatgacgc taggttactt tggtggttcc gttggacagg cgggagcagt
    10681 ggaagtagtc tacgcagtcg gaatgctctt aggcggtgca ctgatcagtt ttataggaac
    10741 atggaaagac cgaatgaaac ctattattat agcctacatc attatgggcc tgacgatcgg
    10801 tgcaagcgga ttgggtccaa acgatagtca agggttcttg tactttctta tcttgaatgc
    10861 aggagcaggt tgtgcaacgc catatttcaa tacactgctg atggcaatga tccagcagag
    10921 ttatgaatcg aatgttttag gtcgtgtctt agggaacttc aactctttga tgaacctggc
    10981 aggtccaatt ggattattat ttgcaggacc attagctgat cgtttagggg tagaaaaaat
    11041 gttcttgttt tctggaatcg ggattttatt atgcggaatc gttttatttt taacgtcagc
    11101 tgctcgaaat tatgataaag aattacagaa aaaattggta aaagaacacc attagtaaaa
    11161 agacgaatag tatggaatgg agttgtgaca gaagtcttgt tacaactcca tttctgatcc
    11221 taatgttcct gtggcaataa gcctactcaa atgaaaaata tgagtaatta tctttatttg
    11281 tttaatactt gatatagggc agttttttca cgacctatcg tacttcctct ttgcttcata
    11341 gatttctttg atttgttgct cagataacgg agtgccaaat ggtagttttt cctttaagag
    11401 ttccctttcc ggtgcgtcta cgcctacatt gacatcgttc tcgataggta cgctgtaatg
    11461 atggatatgt ccatgaagat tgcggatagt gtcgttaggc ccgagcaaca tcggataatg
    11521 agtcaagtaa tattgttgat ggtcgaattt aacgatggca cctacatcat aaaaataaaa
    11581 tttgggactt ccgtcagtaa gcctaggatc atttttttct aaataacgga aaaaagcgcg
    11641 actgtcatgg ttccctttga tcaagtggat tgttccttgc agttgagtga ccagttccaa
    11701 aatttctgca tttgcttttt catattgcgg atgcatagca aggtcgccta gatgataaac
    11761 atggtcggtt tcttttatta ctgcattcca gctagtgatc aactgttggt tcatttcatc
    11821 cacatttgca aagagccgcg gagcaaagtc gttttttcct aataagcgtt catggaaaaa
    11881 gtgcatatca ctggtaaagt aattcatgta tcttttgcct cccttttttc ttagagtaaa
    11941 caaaatcact tatctgagca actcataaca tctaaaaaga aaaggttgta aatgagacgt
    12001 gtagctttaa gcgataagaa gcaattttga aaaatagttt ttcacaattt ataaaattat
    12061 tccttattgc cgaaaggctg gcccttttta tgagtatgcc aattttttat atcgaatgct
    12121 caaaagatga gtgatttttt ggttgactat catgtttgtg tactgaatag gtccagtaga
    12181 acagtatttt tacgacttga taattaaggg atgaaatttt cagaaatatg acgtataatg
    12241 taaaatactg aaaagttgcg accttcaact cgtagatgtt tcagaatcta attgaaatga
    12301 tggtgatatc atgacagaca acagcaaaac acgcgttgtc gtagggatga gcggcggtgt
    12361 cgactcatct gtaacagcac ttttgttaaa ggaacaagga tacgacgtga tcggaatatt
    12421 catgaaaaat tgggatgaca cggacgaaaa cggtgtatgc acggccacgg aagattataa
    12481 ggatgtagcg aaagtagctg ctcaaatcgg gattccttat tattctgtga attttgaaaa
    12541 agaatattgg gatcgtgtat ttgaatattt cctagccgaa taccgcgcag gacgtacacc
    12601 taacccagac gtgatgtgca acaaagaaat caaattcaaa gcattcttgg attatgcaat
    12661 ggatcttggc gccgattatg tagctactgg acattatgcg caagtcactc gcgatgaaaa
    12721 cggcgtggtc cacatgcttc gtggagtaga caataataaa gatcaaactt acttccttag
    12781 ccaattatca caagaacagc tttcaaaaac gatgttccct ctaggcggca tggaaaaatc
    12841 agaagtacgg gcaattgctg aacgcgcagg ccttgcgact gctaagaaaa aagattcaac
    12901 gggaatctgt tttattggtg aaaagaactt caaacaattt ttgagcaact atctgccagc
    12961 aaaaaaagga aatatggtca ctcttgatgg tgaagtcaaa gggcaacatg ctggcttgat
    13021 gtattatacg attggtcaac gtcaaggact agggatcggt ggcggaggag attcgcaaga
    13081 accctggttt gttgtaggaa aagatctagc gacaaacaca ttatatgtgg ggcaagggtt
    13141 ccatcatcct gcattgtatg ccacaagctt ggatgcaagt gaaatccact ttacgacgaa
    13201 tgaaccaatg ccaaaagaat tcaagtgtac agcgaaattc cgttatcgtc agcaagacgt
    13261 tcctgtgaca gttcgcttat tggatgataa tcatgcagaa gtcgtattcg acgaacctgt
    13321 acgtgcaatc actcctggac aagcagttgt attttatgat gaaatggaat gtctgggtgg
    13381 tggtttgatc gaccacgcat atcaagaaac aaaagtttta caatacgttt aagcgatatt
    13441 aaatgaatca aaacagctat ggctaacaaa agaaacacct tttcttttgg atagcatagc
    13501 tgtttttttg caataaaatt aatttttatt cattttatac ttgaaatact tgtgtaaaaa
    13561 gttcataata tccaataata tgaataaagt tgctttttgt taagcaagag cagttgcaaa
    13621 atagaaggaa atcttttgca agtgatttgt tcaattttat aagtgagggt gatggaaatg
    13681 gaaaagaaat caacgggtat tagcaaatgg ggactggttg ctctggttgt cagttcgtca
    13741 atcggaagcg gtgtgtttgg gatcacaagt gatctggcta gttctgcagc tccagggcca
    13801 gcgatccttt catggataat cgttgggatt ggtattttag ctttggtatt atcgctgaat
    13861 catttaggag aaaaacgtcc agatctagat ggtggtattt tcggttacgc ggaagcttca
    13921 tttggtaaat taggtggctt catcagtggt tggggttact ggttgtctgc gtggctggga
    13981 aatgttgctt ttgcaacgat gctgatgagc gccatgggag aatttttcgc gatattcaaa
    14041 ggtggacaaa atgttccttc tattttattt gcaagtattt ttatttgggg tctgacttta
    14101 ttagtaaaca atggagttga aggagcgagc gtcatcaata caatcgtgac gatctgtaaa
    14161 ttagtgccat tattcctatt tttaatattt gtatttatcg catttaaagt gaatatattc
    14221 actgctgatt tttggggtaa cgtatctgat aatctagtga atggaacagg gcagcaagga
    14281 acgatctggt tgcagattaa aggttgtttg atggttatga tgtgggtctt tgtagggatc
    14341 gaaggcgcaa gtgttttagc caaccgcgcg aagaaacgtt cagaagccca gcaagcatcg
    14401 atcattggtc tgctttgttt attgtttatc tatatccttg cttcgatctt gccatacggc
    14461 gtcttatctc aagaagagtt agcaactatt tcacaacctg ctatggcaaa tattttaaaa
    14521 ggaatcgtgg gaaattgggg agcagcgctg atcaatatcg gcttgattat ttcgatcatt
    14581 ggttcttggt tgtcttggac gatgctccca gcagaaacaa cgatgttgat ggctcgagac
    14641 ggtctgttgc cgaaaggttg gggaaaaacg aataaaaaaa atgccccaac ttattcgtta
    14701 gttgttactg caatcttgac taatctattc ttattgactt tcttagttac tgattatgcc
    14761 tatcaatttg cctattcttt atgtacagcg gcaattttga tttgttatct acttgtcggg
    14821 atctatcaag tccagttttc tttaaaagaa ggaatcaaaa aacaagtagt gatcggtggg
    14881 attgctgtag cttttgaatt gatgggcatc attttagcag gattttctta tgtcctttta
    14941 tgtagtattg cctatcttcc tggtttttgc ttctactgga tcgcttgtcg tgaaaccaaa
    15001 catcaaatta cgggaaaaga aaaagtaatg attgggctga tttgtgtggc cgcactgctt
    15061 tcaatcgggt tgcttgctgt aggctggatc aagatataag ggggaaagaa acatgaaaat
    15121 cgcaaatttc ggtgtagaag aatggctaaa taaatgggaa aagaaagcgg tatatgatat
    15181 tgcacaaagc tcgatcgaag cattgacatt agacgaactc gttggcttag atgggactag
    15241 cgtaagtgaa tttttcgaaa aacgcaaaaa cgaaccattg gactatggct ggatcgaagg
    15301 atcagatgtt ttcaaacaag aagtcgcttc tctttatcaa accgtcgatc cggaaaacat
    15361 cttacaaacg aatggagcga ctggtgctaa tcttttagct ctatacgcgt tagtggaagc
    15421 aggagaccat gttgtttcga tgcttcctac ttatcagcaa ttatatgaca tccccaaatc
    15481 tttaggtgcc gctgttgact ttgttcattt gaaggaagaa gaagattggc agtttgattt
    15541 agaacagcta gaagccttag tcaaacctga aacaaaaatc atctgtttga atagtgcaaa
    15601 caatcctaca ggcacattgc tagatcggaa aactttagaa aaaatcgcag aaattgctcg
    15661 tacagtagat gcatatgtat tgatagatga agtatatgcg cctttaacgg ataaaggtga
    15721 attcttgtcg atcgtagatg tctatgaaaa aggtatagca accaattccc tatccaaaac
    15781 ctactccatt ccaggtattc gtattggctg gacagctact ggacctgagt tggcagaagt
    15841 ttttcgaaag tatcgagact acacgatgat ttgcggcgga gtcctgtcag atgatcttgc
    15901 tgtacatgca ttgaagaata aagaaaaaat attggagcga aacaaaaaga tcattacaga
    15961 gaaccttgtg attttaaaac aatgggtagc caatgaaccg aaagttgaac ttgtcgctcc
    16021 caattacgtc tctacttctt tcattaaatt agccatcgaa gaagatgacc agacattttg
    16081 tatcaattta ttagaagaaa caggagtact attagtacca ggaagtgcct ttgatttacc
    16141 gaagcatgcc cgactaggtt actgttgtaa aaaagaaacc ttggaaaagg gattattcct
    16201 cttatctgag ttcttgaaaa aacaggccta aacaaaaaca ctgcaaggac agtctgtgac
    16261 aactgttcaa gcagtgtttt tcatttacgc ttattgtcta tgagaaggag agtgccctga
    16321 agatatggag actcctgaag ctttttcttc tgcagtttct tgctgatgga tctcttgtgt
    16381 attgtgtcgt acaagcaaga agttgaccaa aacaagaaga gaagtcgaaa gaaagacgcc
    16441 acgataacca aatgcactag aaacactgga gccgatcata ggtcctacta cgttaccagt
    16501 tgcttggaaa gattggttat aactgaagat ccgtccagca gcttgatgag gagaatattt
    16561 tgtgatcaat gcttggacag cagggagcag acaagcatca gagataccca tcaaaaaacg
    16621 taaggcagct aattgccaaa cattcgtgac gaatgccatt gggatataaa ccagaatcgc
    16681 aaaacccaaa ccaatcgcaa gtattctttc actcccaatc ttgtcaccta agcgaccaaa
    16741 tcttggagcg gctatcaaag tggcaatccc aggtatcgaa gcgatgattc cgctgatcaa
    16801 cgtaacattc ccatgtccat gcagcaattg gcgaatgtac agactgatga ttggactaat
    16861 ggaattgttg gatgcttgga tgatcatcgt tgtgacgaac ataccgatga cgacgtgtgg
    16921 atatttcaag tctttgaaaa tctgtttggc agaaaccatg ttttcttttt tcactgggac
    16981 aaatttttca tggacgaaaa acagactcaa caagaagacg aaaaataaga tgatccctgt
    17041 aatgaaaaag gtagggcgat agccaaacgc tgaagcagag atcccgccaa gaagcggtcc
    17101 aagtaatgtt cctgtaacag aaccagttgc taaagttcca agaacttgac cacttttttc
    17161 ttttggcgtc ccagttgcga caagggcagc ggcattactg atataaccag aaaaaatccc
    17221 ttgaagcaga cgcaatgcga ctaattgata cacactagtc acgcaaccca tcaaagaaat
    17281 gacgattgcc attcctaatg aagcacgcaa cagcatcaat tttcttcctt tttggtgggc
    17341 taattttccc caccaaggag agatcagcgt agtcaccaag aaagtagatg aaaaagtcaa
    17401 tccgctccaa aagttcaatt gtgacgtact ataattacct aacgtatcta tgtaaagtga
    17461 cataaaaggc atgactaaac taaatccgat accagccata aatgttccaa accataagac
    17521 aaacaagttt tgttcccaag tttctctttt cctaaataat ttttccttta ttcttgcagc
    17581 caattagact ccttctttct gaaaattttc atctacagct ttttgttata aactacaata
    17641 tgtagtttat caatgaaagt ctcttttttc tctttctttt tcgtggtaaa ctgaaagagt
    17701 gaaggattta ttttatgaga ggaaacatga aaatgaaaaa aagaaatctg tctcaagaga
    17761 aaatcatcga ctgctttcga gaactagcag aagagatgga tgttcagcaa ataacctttc
    17821 agcatttagc aaaacgcttg gatatcaagg ctccctcgct gtataaccat ttcaaaaata
    17881 ttcgtgatgt caaaacagcg ctaacagcga aacttctgca ggaacttaat gaccagctgc
    17941 gtcgggctct tgtgggaaaa agtggtcagg aagcactgca gatctatgca gaaacttatc
    18001 gatcctttgc tttttcaaat caagcagtgt atgagttact gatcagtgtg ccccacacca
    18061 atgaagcagt tttattagag ggaatccatg aaacgaacca gatcattttg cagatctttg
    18121 aggcttttca attaacgaaa gaagaaaaag tccaccgaag cagagaatta tgtagtatga
    18181 tccacggcta tctttccttg cgttttttag gatactttac aaaagaatcg acaatcgatc
    18241 cagaggacag ctatttttgg atgatcaatg actttatcgc tactttgcct taaacaatca
    18301 gattcattta tatcattttc aagatgttca ttaaatgatc cttttataag agaaaattct
    18361 tgcaactttc ttgaaaagtg actaagataa gtctagaaaa gctgaaagaa ggagcacgag
    18421 tatgtatcaa gtaattacca tgttcgggga caatgagcct tggtggtttt ttgaagactg
    18481 ggaagaagat attgaagcag aagaaacatt cgagtctttc gaagaagcac gacaagcgta
    18541 cgaaaaacaa tggaacgaaa tccatcaaaa ttatgaatat atcaatgcaa aaagtaactt
    18601 tctcagcgcc ttttggaatg acggagatga gcgctggtgt gaagagtgtg acgatgattt
    18661 acagcaatac aaaggacttg cgttgttgaa aaatcatcag cccatcacag tagaaagcgg
    18721 aaaggaattt tatgaaacaa ctaattctag cggaaaaacc aagcgttgcg aaagacctaa
    18781 gcaaggtgct tggtgctaat caaaagcaca aaaattatta tgaaggaccc aaggtgatcg
    18841 ttacttgggc ccttggtcat ttattaggat tgaaaatgcc ggaagatctg aataaggaat
    18901 ggcagagctg gcaaatggac acgttgccaa tgatccctaa aaaactagga atcaaaccac
    18961 ttcctaaaac aggacatcaa ttaaaagcaa tcaagcagct ggctcagcga aaagatgtct
    19021 cagaagcagt cattgcgaca gatgccggaa gagaagggga acttgttgct cgctggatct
    19081 tagagtatgt gcatttcaat aaaccagtga agcggttatg gatctcttct caaacagata
    19141 aagcaatcaa aactggattc aagcaactaa gacctgcgaa agaatatgat gcattgtatg
    19201 aatctgctct tgcccgggca aaagcggact ggctggtagg attgaatgtg accagagcct
    19261 taactgtgaa gtacgcggat aatttatcag caggccgtgt gcagacaccg acactagcat
    19321 tagtgagaga ccaagaaaag aaaatcgagc agttccgtcc acaaacgtat ttcacgatca
    19381 cgttgcaagc tggaaaagaa caagccaaaa tgcttcaaaa aaatcaattt gctttaaaaa
    19441 cacaagaaga agcacaaaaa cttgttcaat tgatcagtac aagcaaagga actgtcacag
    19501 aagtccaaga aaaattaaaa acggaatctg cgccattgcc ttatgattta acagagatcc
    19561 aaagagaagc caatcaacgc tatggctatt ctgcaaagaa aacattaagt cttgtacaaa
    19621 gtttgtatga gacccataag atcgttactt atccgcgtac agacagcaaa tatctgacaa
    19681 atgacatgaa aagtacgatg aaagaacgat tgcaagcagt cagcgacttt gctcctgagg
    19741 tcaagcagta tttgaaaaac ggagccatcg tccaacagac aaaagtattc caagacagta
    19801 aagtgaccga tcaccatgca cttttgccaa ctgaaaacag agcacgttat gaaaaattaa
    19861 gtaatgaaga gcaaaaaatc tatcaaatga ttgtctctcg ttttttagga ctgtttgctc
    19921 aaccccataa agtcagccaa acaaaagtta cggttgaatt tggtaaagaa cgattcattt
    19981 ttcggcaaaa tcgtgtcgtc caagcaggat ggaaaggcga aactgaaact gagacggaaa
    20041 cagtcaaatg ggaaaaagaa atgagaatca atcccgattt caccatcaaa aaagaactat
    20101 ctgcaccgcc taaaccattg tcagaagcaa gcttgcttgg tcaaatggaa aaatacagtc
    20161 tgggaacccc agctacgcgt gcagagatta tcgagaaact aatcaaatct gaactgatgg
    20221 aacgtacccc tcaaggactg caagtatctc ctaaaggtaa gcaattatta gtattggtaa
    20281 atccctcttt agtaacacca gatttgacag aaagctggga aaaagacctt gaagctattg
    20341 ctgcaggaaa gaaacaagca ggaaccttct tgaatggaat cgaaaaagaa acaaaacgct
    20401 tggtgaatga gatcaaatca agcaaacaag aatatcaaga tttctcgatt acccaaaaga
    20461 aatgcccaga atgtggggcc aatctgcgcg agaaaaacac acgtgacggc aaaatctatg
    20521 tctgcaccaa ttcagattgc aactaccgca gaagaaaaga tccaaaagtc tctaatcatc
    20581 gatgcccgca atgtcaccgg aaaatggaaa tcatcgaagg taaaaacggt gcttatttcc
    20641 gttgtaaata cgatggaacg acagaaaaaa tgcttgataa aaaagagcag aagaagaaaa
    20701 tgaccaaaca tgaagaacga cgcttgctga aaaaatatgc acaagcagaa gaaccagaag
    20761 aaagcccatt agcagcagca ctaaaagcag caatgaagaa gtaataagat atgaaagagg
    20821 ttgtgataga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa
    20881 atttttgtga aatttcggct tatttccgaa gaagctactt ctggaacacc atttattcgg
    20941 aaaaaaggac tgtgacaaga cttttgtcac agtccttttt catttaaaaa aatgtttact
    21001 tcatttgacg tatattattg gtgaggcttc aattcatttg gcttcacttt ttctgtttct
    21061 cttatgatat aaacaggacg ctgtttcgtt tctaagaaga ttttcccgat atatttcccg
    21121 atgattccaa gacataacaa ttggattcca ccaatgaaca ggaagatcga gaccatggaa
    21181 ggccatcctg atgtaggatc accaaacaac aatgcccgca aaataatgaa caaaattgca
    21241 ataccagaac cgacacaaga caaagcgccg ataaatgaag caatcgttaa cggtgcatcc
    21301 gagaagttca cgataccatc aatcgagtag ttgaataatt tccagaagga ccaagatgtt
    21361 tcgccagcca cacgttcgcg gttttcataa gaaatatatt ctgttttaaa tccgacccag
    21421 ctaaataatc cttttgaaaa tcggttgtat tctgttaatt ctaggacggc atccaccatt
    21481 tgacgagtca tcaagcggaa atcacgagcg ccatcgacca tttctgtatc gctgatttta
    21541 ttgatcaact ggtagaattt tttcgcaaag aaactgcgta tcgcaggctc gccttcacga
    21601 gtcgaacgcc gggtgccgac actatcgaca tcagaagtag tgatccggtt gatcatctca
    21661 ggaagcagtt cgggcggatc ttgcagatca acatccatca cggtcactaa atctcctgta
    21721 gaagcttgca atccagcgta cagagccgct tctttcccaa aatttctgga aaatgagaca
    21781 aagcggacat aaggataagc tgaggctagc ttcttgattg ctgctaacgt accgtctttt
    21841 gatccgtcgt caataaacaa atattcgaac tcatgggcac ctttgaattt ttcaacctca
    21901 tcaaaaaaaa gaggaacagt tttttcttca ttaaaacatg ggacaacaat tgatactttc
    21961 atcgtttcta tcctttcaat cgtatgttta tatatggttt acattcaaaa tttaataaac
    22021 cttaaataac accttttaaa tttgatttta tggaaaagaa cagctattct ttggtaaact
    22081 aacgtattta gtttaccatg tttcctcggt ttatcaaact tttaagaaca atttttatta
    22141 gttggcaagt agttttggat agagaaaaag gaggaagttc cagtctactg accaggagca
    22201 aacaaaatga aaatacacac acctatcggc tttttatggt tagatgcaga tgaatccgga
    22261 ttgactgctg tatcttttca gccaatttca gaaagcgaat cagcgaatca gcaaatttta
    22321 acgcgcacac aagaacagtt tgaagaatat tttgcgggaa aaagaaaaga atttgatgtt
    22381 cctttatcta taaagagagg tacgcctttt cagcaaaatg tctggaaagc tttgtcgacc
    22441 attccttatg gagaaatccg aacgtataaa gaagtagctg ttgctgttga tagtccgaaa
    22501 gctgtgcgag cgattgggca ggcaaatcga accaatccat tgccaatcgt tattccttgt
    22561 catcgagtga tcgggcaaaa tggaaaactg acaggatata tgggaaacgc aatagatggg
    22621 gttgaaatca aacgccaatt gttgttgtta gaaggatatc tgcaaaatta aaaacagttg
    22681 tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca ttctaaactt
    22741 tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca gctaatccat
    22801 aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc agaccaattg
    22861 ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg tcaataacta
    22921 caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat acataccaag
    22981 gaagtggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt
    23041 tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata atacgtagaa
    23101 tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc ccagcgacta
    23161 cttgaccaac agattggagt gtgccaccca aaagtgcact tttttcaatg agatttccac
    23221 caaacaactg taatccaagg aaagggagag tcaacatcat cacagtaccc agtagattga
    23281 ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg gcgatcgaag
    23341 caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa gaagtatcgc
    23401 tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac atttgcagta
    23461 acacaaaaac aattcctttt ataccaattt gagcaatcgt ttgagctgtg acagttgcac
    23521 ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg ccgattgcta
    23581 gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata gcaatagtcg
    23641 ctctacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg atggcaaaag
    23701 aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat tttattggat
    23761 gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag catactcgaa
    23821 catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt
    23881 cacgacctct tgtttcgaat aaacggtg
//
LOCUS       DAZKCN010000041        23637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000041 DAZKCN010000000
VERSION     DAZKCN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.042"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..442
                     /locus_tag="ACG8K3_001433"
     CDS             5..442
                     /locus_tag="ACG8K3_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145316.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            537..1421
                     /locus_tag="ACG8K3_001434"
     CDS             537..1421
                     /locus_tag="ACG8K3_001434"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP9145317.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1645..4812
                     /locus_tag="ACG8K3_001435"
     CDS             1645..4812
                     /locus_tag="ACG8K3_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9145318.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4880..5377
                     /locus_tag="ACG8K3_001436"
     CDS             4880..5377
                     /locus_tag="ACG8K3_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFP9145319.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5396..5860
                     /locus_tag="ACG8K3_001437"
                     /pseudo
     CDS             5396..5860
                     /locus_tag="ACG8K3_001437"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5857..6615
                     /locus_tag="ACG8K3_001438"
     CDS             5857..6615
                     /locus_tag="ACG8K3_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP9145320.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6701..7441)
                     /locus_tag="ACG8K3_001439"
     CDS             complement(6701..7441)
                     /locus_tag="ACG8K3_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP9145321.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7502..8755
                     /locus_tag="ACG8K3_001440"
     CDS             7502..8755
                     /locus_tag="ACG8K3_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP9145322.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9077..9304
                     /locus_tag="ACG8K3_001441"
     CDS             9077..9304
                     /locus_tag="ACG8K3_001441"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9145323.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9427..9753
                     /locus_tag="ACG8K3_001442"
     CDS             9427..9753
                     /locus_tag="ACG8K3_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP9145324.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9821..10534
                     /locus_tag="ACG8K3_001443"
     CDS             9821..10534
                     /locus_tag="ACG8K3_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP9145325.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10844..11695
                     /gene="licT"
                     /locus_tag="ACG8K3_001444"
     CDS             10844..11695
                     /gene="licT"
                     /locus_tag="ACG8K3_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP9145326.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11816..13681
                     /locus_tag="ACG8K3_001445"
     CDS             11816..13681
                     /locus_tag="ACG8K3_001445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9145327.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13695..15138
                     /locus_tag="ACG8K3_001446"
                     /pseudo
     CDS             13695..15138
                     /locus_tag="ACG8K3_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15246..15947
                     /locus_tag="ACG8K3_001447"
     CDS             15246..15947
                     /locus_tag="ACG8K3_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9145328.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16073..16585
                     /locus_tag="ACG8K3_001448"
                     /pseudo
     CDS             16073..16585
                     /locus_tag="ACG8K3_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(16983..17192)
                     /locus_tag="ACG8K3_001449"
     CDS             complement(16983..17192)
                     /locus_tag="ACG8K3_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP9145329.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            17432..18007
                     /locus_tag="ACG8K3_001450"
     CDS             17432..18007
                     /locus_tag="ACG8K3_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP9145330.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            18007..18447
                     /locus_tag="ACG8K3_001451"
     CDS             18007..18447
                     /locus_tag="ACG8K3_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9145331.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            18463..19164
                     /locus_tag="ACG8K3_001452"
     CDS             18463..19164
                     /locus_tag="ACG8K3_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9145332.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(19652..20077)
                     /locus_tag="ACG8K3_001453"
     CDS             complement(19652..20077)
                     /locus_tag="ACG8K3_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145333.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            20451..21422
                     /locus_tag="ACG8K3_001454"
     CDS             20451..21422
                     /locus_tag="ACG8K3_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9145334.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            21454..22851
                     /locus_tag="ACG8K3_001455"
     CDS             21454..22851
                     /locus_tag="ACG8K3_001455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9145335.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIKF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            23010..23633
                     /locus_tag="ACG8K3_001456"
     CDS             23010..23633
                     /locus_tag="ACG8K3_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145336.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         7999 a         3907 c         5023 g         6708 t
ORIGIN      
        1 ataaatggag tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt
       61 tgtttttctc acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat
      121 tcatcaattt tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat
      181 tattcaaggc cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat
      241 tcctctgatc gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt
      301 gctatttttg tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct
      361 atcattttct tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat
      421 gttttatctt gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat
      481 ttttttcttt atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga
      541 aaaaagtttt attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc
      601 aattagcgat agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa
      661 aagcagggga tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt
      721 ttgtcatggg cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac
      781 atcggccaaa tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag
      841 ggataccgct agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat
      901 tggatattgg aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc
      961 ctgaatcaat caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt
     1021 tcatgtcagg aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg
     1081 atggaaagaa ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg
     1141 gcggttttga aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt
     1201 atttaaaaga ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag
     1261 tagatgaatc tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag
     1321 ctgatgagga agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga
     1381 caatcaaagt attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag
     1441 gagttggcgg gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca
     1501 gccttttttt tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata
     1561 catattttaa atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca
     1621 aaacgtcaga gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt
     1681 ttgtttgtct tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac
     1741 gaatcaacgc aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt
     1801 acttctgaaa ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat
     1861 aagacaagtg agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg
     1921 gcaagagctg tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag
     1981 cttctcgcag acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg
     2041 gaattagaga tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc
     2101 acacaccata tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga
     2161 acagctgcac atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct
     2221 tatatgaccg atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg
     2281 acacttgatt tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc
     2341 acattagatg gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc
     2401 gctccgggta aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa
     2461 ctttatggac cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt
     2521 agtggaaaaa cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat
     2581 gatcgggcag atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc
     2641 atgtttgaag tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc
     2701 aatgacaaca atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg
     2761 aatggtggga aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat
     2821 tctagtcaaa tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac
     2881 gatcaacccg ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa
     2941 ggagccggat cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt
     3001 gagaatggta cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca
     3061 ggtgatggca aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat
     3121 gacgcttctg gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa
     3181 tcaaatatcg atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat
     3241 ttaaatttta cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat
     3301 aagaccggca tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt
     3361 gatttccaaa acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa
     3421 ggaaatggag attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca
     3481 ggcgcaacat tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta
     3541 tcgatcaatg ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat
     3601 gaatttgctc agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag
     3661 ctcccgatga ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat
     3721 gtcgttgttc ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa
     3781 gtgactttac gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc
     3841 acgcaaggac aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa
     3901 caaggcggct tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa
     3961 gtgcaagttg ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct
     4021 gccaaaacga cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc
     4081 aatgcagctt ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa
     4141 aaaaatcaag ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct
     4201 atcccagaaa atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca
     4261 ggagaagcta ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat
     4321 cacaatccaa gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg
     4381 aagatcgtag tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag
     4441 atcaaagcaa ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta
     4501 tggatttatg atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag
     4561 cctttcacaa atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag
     4621 gagtatcaat tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat
     4681 gtgcaagcag attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc
     4741 aaagaaaatc agatagctgg taattactca gcagagataa catggacatt agcagatgcg
     4801 ccgacagaat aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg
     4861 aaacaactgc tctttactta tgatccttct ccttggattc atcctttggc agacacctgt
     4921 ccatgcctcc gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa
     4981 gaacaaaaca tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac
     5041 ggtctttaac cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac
     5101 gaattcaaac ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag
     5161 ttatgatatg aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc
     5221 ggtcactgag acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg
     5281 agggatcacg tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt
     5341 caatcagttt tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc
     5401 agatttgaaa ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc
     5461 gaatcttcaa aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta
     5521 caaaaaagaa acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc
     5581 caatacaaat tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta
     5641 tcaagcggtt gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga
     5701 aataactgat gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc
     5761 agaaacattt gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc
     5821 tggattattt attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt
     5881 accgttctag ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc
     5941 tcagccacta cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac
     6001 gttgttccag ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa
     6061 ccaaatcagg gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga
     6121 acaatcaagt taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt
     6181 actggagcac aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat
     6241 gccggttgga aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat
     6301 aaactaactg gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat
     6361 agcagtggaa tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca
     6421 gatcaggcag tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat
     6481 tcattaggag agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc
     6541 aattatgctg tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca
     6601 gaagaggaag cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac
     6661 tccctgaaat ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt
     6721 ggaagtcatg gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt
     6781 caccgttgaa aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt
     6841 tgatacagta agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt
     6901 gttttaaaga agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt
     6961 tcaaaccttt gatactttca atatataaaa tttcccgagg atacaggacg agacttccac
     7021 ctttttgatc aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct
     7081 gttctagcac ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg
     7141 gaaggatttt ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa
     7201 tattactatg tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt
     7261 gcttcaagtc gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca
     7321 actgattgat ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca
     7381 attctctgat gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca
     7441 tatgcaggct cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa
     7501 gatggtcgca acagtgacac tttatttgac ctttactttg attttagatt taggtatcta
     7561 tttattcttt tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt
     7621 ggtgacactt ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt
     7681 gatgtttctt ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct
     7741 tttttttgct atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg
     7801 aaagagacat cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct
     7861 attagtaaaa agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac
     7921 tgaacactat tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt
     7981 gggaagtgtt cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat
     8041 cagttttttt tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca
     8101 acatcaggaa tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga
     8161 attcaaacat gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa
     8221 aaaagaaacg ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa
     8281 accgcaaata caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa
     8341 atttctagaa aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt
     8401 gaaaacaatc ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa
     8461 cgcattcgat catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa
     8521 agagggatca cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga
     8581 gatggtcaaa agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa
     8641 aaaaatgttg gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca
     8701 ctattttttt gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac
     8761 tgtcaaagga taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt
     8821 caaaagaaac agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta
     8881 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
     8941 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
     9001 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
     9061 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
     9121 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
     9181 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
     9241 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
     9301 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
     9361 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
     9421 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
     9481 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
     9541 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
     9601 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
     9661 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
     9721 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
     9781 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
     9841 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     9901 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     9961 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
    10021 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
    10081 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
    10141 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
    10201 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
    10261 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
    10321 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
    10381 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
    10441 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
    10501 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
    10561 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
    10621 aacgcaaaga ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt
    10681 tttatttttt atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa
    10741 taagaaatgg attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg
    10801 cgaattctag gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat
    10861 tttgaataac aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg
    10921 aaaaggacta gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa
    10981 agaatttgtt ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat
    11041 tccggcagag gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa
    11101 agttgaattt tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac
    11161 acgagcaaaa gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta
    11221 tccgaaagaa tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt
    11281 ggaactttct gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg
    11341 aaacgaagac atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat
    11401 catcagtaaa ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat
    11461 tactcatctg cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga
    11521 tgaatttctc tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg
    11581 gatcaatgag tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta
    11641 tttaacgatt catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt
    11701 gttactgtat ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg
    11761 tttttttcaa tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga
    11821 taatcaagca gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag
    11881 tttagtacat tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac
    11941 gcaaaagtta aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca
    12001 agtagtgatt ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct
    12061 tgcagtttcg gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat
    12121 tatctctagt atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg
    12181 attcttatca ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat
    12241 attgtttgct gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc
    12301 agctaaaaag ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta
    12361 tccagctatc acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt
    12421 gattctttca ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt
    12481 tcaaagtaaa ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt
    12541 agttccgctt gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat
    12601 cgggacggtt gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc
    12661 aatcgtagca ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca
    12721 tcggggattc gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat
    12781 gccaatgctt cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg
    12841 aacaaaagat acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg
    12901 aatcactgaa ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc
    12961 ttgtatttct ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc
    13021 cagcagtttg gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga
    13081 gtcaaatgtg acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg
    13141 tacattgatt ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc
    13201 gaatgcagga gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa
    13261 agtactgcca ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg
    13321 tatcgctatt gatccagaag taggagaact agttgcacct gcagacggag agatcacgac
    13381 tatttttcca actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat
    13441 tcatatcggg atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca
    13501 aggggatctc gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc
    13561 agctggctat agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat
    13621 acttgaatta gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata
    13681 aggaggaaat caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg
    13741 ctgccaaatc aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat
    13801 gctatgcctg gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg
    13861 attgatcaag aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc
    13921 aaagaagatc ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc
    13981 tggacacgaa tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt
    14041 tatgatcagc tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct
    14101 cattacgaaa tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg
    14161 atcgacttct atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa
    14221 tattggatga ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga
    14281 ttagtaaaaa gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa
    14341 ttcgttgcaa gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc
    14401 ggctgcatga ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca
    14461 gcaacaatga tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga
    14521 gaataccctg catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta
    14581 gagcaaacgg aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt
    14641 agttattata tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg
    14701 aacttgctgg gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc
    14761 gatccagaag ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta
    14821 ttcattgttg aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt
    14881 gatgattatc ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca
    14941 gacggtgtag atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca
    15001 acaggagaaa tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat
    15061 gggacactga atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca
    15121 aatggcgtaa acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga
    15181 tttgtgttct ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg
    15241 acgttatgaa aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata
    15301 ttttctcagg aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac
    15361 tgtttcaagt cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat
    15421 atgtcgcaaa gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat
    15481 tatccaaagc ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag
    15541 aaacgctgga tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg
    15601 gtgagcaagc tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt
    15661 ttgttcatta tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat
    15721 ctttgtatcg tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct
    15781 cggcagtatt tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca
    15841 tcaaacggat acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg
    15901 tctaccatac ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg
    15961 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    16021 atttttcgtt tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta
    16081 ttctggataa gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt
    16141 gattttagct tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat
    16201 cattcgtaaa aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat
    16261 ggggatctgt cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa
    16321 tgtcatagca acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc
    16381 agatagccgt attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc
    16441 attcattggt gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa
    16501 aaaaccagta gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc
    16561 ttgggatgat ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat
    16621 agatgtacct tgaggcagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac
    16681 agattttggt ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac
    16741 gatgaaggaa aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag
    16801 gaaaggaaac aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag
    16861 taaaaaaaga aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac
    16921 actagttcca ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg
    16981 attcaagtga tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga
    17041 agctgatgaa caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga
    17101 gaaacatcat ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa
    17161 gaaacaagta tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag
    17221 tatcttctat aagtaagtca tttcacatcg atgaaaacac gttatttatt tttgttttat
    17281 ctataaaata gctgaaaaat gtttttttct aaatactata tatagtggtg tctatcctat
    17341 ataccactaa aaaactacaa aatctagtct tttattttaa aaaaacacgt gtagaatgaa
    17401 gatagacttt tgagttaggg agtgtttttt tatgaaactt actgtttttt gcggttcccg
    17461 ttttggtaat aaagaaagtt acaaatttat tgcgcaaacg ttaggaaaat acatggcaca
    17521 agaagaaatc gaactggttt acggtggttc atactccggg attatgggag tgattagtaa
    17581 aacagtactg gaaaataacg gaaaagtcac aggtatctat ccgaatgggc tttttgaaac
    17641 tgaattgcct ggaaaagatg ttactacatt tattcctaca gaaacgatcg atgaaagaaa
    17701 ggtattatta tttgaaaagg gagatgctgt cctcgttttc cctggaggtc taggtacatt
    17761 ggaggaattc tcacaacttc tttcttggat agcaatcggt ctgacgcccg acaagccaat
    17821 cggaatattg aatattggcg gttattacag cggcttgcaa caattattag aaacttttgc
    17881 aaaagaggat tttatggata aaaaatggtt ggatcaagta atcttttcca acaatccatt
    17941 ggaactggtc aatgaattac gagcagtatc tattaaaaat cagttattat tgaaggaggc
    18001 aaaataatga gtcaaatata tttagcagga ccattttttt cagaggagca aatcgatcga
    18061 gtaagtcgta tcgaaaaagc gttggaggaa aacaaaacag tcacaagttt ctactctcca
    18121 cgtcatcacc aagaaagcaa ttatgaacta ttcagcgctg gatgggcgca ggaagtatac
    18181 gaaaaagata tggaagaact gacaaatgca gaatttgttg ttgctatcct tgactttgag
    18241 catcaaacaa tcgatccagg aacggcttac gaattaggtg tggcaactat gttgaaaaaa
    18301 ccgatgatca ttgtacaaga agagacggta cctacgaact tgatgattac acaaagtctc
    18361 catacgtatt taaaatcaga ccaagctgta cgtgaatatg attttgaaac attgcctgtt
    18421 gaaacttacg taggcgagta cttatagaag aggaagggtt gtatgtcagt gattttgatt
    18481 tcaggaacga ttggtgcagg taagagcagt ttgacggata tgcttgcaaa agagattgat
    18541 tctaaaccat tttatgaaaa tgtcgaagat aacgaagtat tgccgctctt ttattcaaat
    18601 cctgagcagt acgctttttt attgcaaatc ttctttttga acaagcgatt tttagcaatg
    18661 aaaaatgctt tagtgaacga tgacaatgtg ctagaccgat ccatctatga agatagcctg
    18721 ttgttccatc taaacgctga tttaggtcgg gtaacggata tcgaagtaca gcagtacgat
    18781 agcttattag atacgatgct aaacgagtta gatgatgttg ctccaaaaaa acggcctgat
    18841 ttgatggtcc atatcaaagt ttctttggac acgatgctag aacgtatcaa aaaacgtggg
    18901 cgtgactatg aacagctaga aagcgatgag acgttatata cctattatga aacgttgaac
    18961 acccgctata atcaatggta tgaagatttt gatgtatgtc ctaaaataca agtggatggg
    19021 gataaattcg attttgtaga aaatccagaa gatgcaaaaa aggtgatccg tcagatccaa
    19081 gaaaaattag ctgaattgga aggaaagcct gctggaaaat attttacgga taaccagcat
    19141 gcaaaaggtc aagaattagg ctaaaaataa aaaaccaaac gacgggaaag aaaaattact
    19201 acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat atttgaaata
    19261 aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt ttactgatga
    19321 agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat agtgagtttt
    19381 gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat aagattcaaa
    19441 tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca agcttccttg
    19501 gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat caggactatc
    19561 gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca gaaaagaagg
    19621 ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg ccatcctcat
    19681 ccttcaaacg gtaattaccg attttggtaa atgtgatggt tgcttctttt ttattttctt
    19741 ttcctgaaat aatatattcg tcatctttgg tagtaatctt gatgtctgtc agcactgttt
    19801 tttcttcaga tgattcagaa gaatcagttt cttgtgtctg atacgtgcgt gtccattcca
    19861 tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta cgtactttta
    19921 gcgtatatac actgttagtg atatctgctt tttccgcttt taagacagaa gaatcggttg
    19981 tttcatccga ggattggtca gagccgccaa acaaattaca gccagtcaat gtcaaaagtc
    20041 ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca tttgatagga
    20101 taaagaaatt ataccattgg aaagtaaaac ttcaaacgga tggaagagaa aaacgcacaa
    20161 aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa aattcgccat
    20221 tttaggaaag cgtttaaaaa aagtgttgca atcttttaaa aaaatgctat attaaaaatg
    20281 aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc tctactgaag
    20341 actttagtta tatggatatt gatgacaagc aacactatgc aaagaaagcg gtcatcataa
    20401 tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt atggcatcga
    20461 tccgtgacgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt tacttgaatg
    20521 gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca gcgttggact
    20581 ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct attggacttt
    20641 tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc gaaagagtag
    20701 ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca gaaaaaattg
    20761 aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc ttcttatccg
    20821 aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt atcgcattga
    20881 attgcccaga aggaccagca ggtattgata caatattagt gaatgatcga gaaagtgtag
    20941 caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt attgctgcca
    21001 ctcaaacaga ctacgtagca agagaacgtt tagcaggagc aaaagaagcg gtacaaaatg
    21061 atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagt ggctattatg
    21121 gggcaaaaga attattagaa aaaggagtaa cagcgatttt tgtcagcaat tataatatgt
    21181 cgatcggtgc tatcgaatgt ttcaatcaag aaggtatcag aatcgggaag gatattgcgt
    21241 ttagtcatta tgattatttg gataaaaacg atttgagcat ccttccgaaa atcacgatta
    21301 caccaccaac agataaaatc ggggaacaag ccgcagaaca gctgttgaaa cgaatcaaaa
    21361 aagaagagga agaaaatgaa aaagtaattg tgatggaaaa tatcatacat ggaatcaact
    21421 aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt tccagcaaat
    21481 ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc aggtgatgga
    21541 aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt ctttgaccaa
    21601 gtaggacctg aaaaaacttc acaagtttat acgaagtatc aagaggatgt acagttgatg
    21661 aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgctt gatacctgat
    21721 gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt tgatgagttg
    21781 attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc gatgtatctg
    21841 caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa atatgccaaa
    21901 agttgttttg aattgttcgg ttcgaaagta aaaaaatggt ttactcataa tgaaccaatc
    21961 gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc agatatgaaa
    22021 aaagcgatcc aagttggcta tcatgaagcg ttggcctcag cgttagccat caaatcttac
    22081 catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc aagctatcca
    22141 agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc gttcttcaat
    22201 cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat tgagatagta
    22261 aaagaacttg atctcatgcc tgtgatccat ccagaggatt tgacggtcat caaagaaaat
    22321 acagtagatc ttttaggtat caattattac cagccacgcc gggtaaaagc aaaagaaaca
    22381 ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta tgaaatgcct
    22441 ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat ctatgatatt
    22501 ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga aaacggtatg
    22561 ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga tgattatcgg
    22621 atcaaatttg tccaatctca tctgaaatat gtccatcaag caattcaaga aggtgccaat
    22681 tgtttaggct atcatatgtg gacatgtatg gataactggt catggaccaa tgcatataag
    22741 aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac aatcaaaaaa
    22801 tcgggtcact ggttcaaaga cgtatcagat aaaaacggat ttgaggctta agaaagaata
    22861 gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag aagttcaatt
    22921 atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata taatagaata
    22981 gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt cttctctttt
    23041 ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga aaaatgacag
    23101 cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca gtagataatt
    23161 tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc aattacgaaa
    23221 aaggcgaatt ggacatctat ttgccaaaag gaaaagaaga aaatcaattt attaatatcg
    23281 atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag catttgaccg
    23341 ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga aaagtgatca
    23401 ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg gaatggaaaa
    23461 agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag tcgacaggaa
    23521 caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa aatcaagcct
    23581 caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa tgaaaaa
//
LOCUS       DAZKCN010000042        23636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000042 DAZKCN010000000
VERSION     DAZKCN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.043"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8K3_001457"
     CDS             <1..435
                     /locus_tag="ACG8K3_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP9145337.1"
                     /translation="GISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIYFALLLG
                     IVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVADLDDA
                     PGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            444..671
                     /locus_tag="ACG8K3_001458"
     CDS             444..671
                     /locus_tag="ACG8K3_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145338.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1307..1678
                     /locus_tag="ACG8K3_001459"
     CDS             1307..1678
                     /locus_tag="ACG8K3_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145339.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1934..2176)
                     /locus_tag="ACG8K3_001460"
     CDS             complement(1934..2176)
                     /locus_tag="ACG8K3_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9145340.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2208..3110)
                     /locus_tag="ACG8K3_001461"
     CDS             complement(2208..3110)
                     /locus_tag="ACG8K3_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP9145341.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3123..3311)
                     /locus_tag="ACG8K3_001462"
     CDS             complement(3123..3311)
                     /locus_tag="ACG8K3_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP9145342.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3325..3888)
                     /gene="amaP"
                     /locus_tag="ACG8K3_001463"
     CDS             complement(3325..3888)
                     /gene="amaP"
                     /locus_tag="ACG8K3_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP9145343.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3926..4819)
                     /locus_tag="ACG8K3_001464"
     CDS             complement(3926..4819)
                     /locus_tag="ACG8K3_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9145344.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4897..5820)
                     /locus_tag="ACG8K3_001465"
     CDS             complement(4897..5820)
                     /locus_tag="ACG8K3_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9145345.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5869..6219)
                     /locus_tag="ACG8K3_001466"
     CDS             complement(5869..6219)
                     /locus_tag="ACG8K3_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9145346.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6252..7154)
                     /locus_tag="ACG8K3_001467"
     CDS             complement(6252..7154)
                     /locus_tag="ACG8K3_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9145347.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7147..8004)
                     /locus_tag="ACG8K3_001468"
     CDS             complement(7147..8004)
                     /locus_tag="ACG8K3_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9145348.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8338..9147
                     /locus_tag="ACG8K3_001469"
     CDS             8338..9147
                     /locus_tag="ACG8K3_001469"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9145349.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9187..9684
                     /locus_tag="ACG8K3_001470"
     CDS             9187..9684
                     /locus_tag="ACG8K3_001470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP9145350.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10069..10142)
                     /locus_tag="ACG8K3_001471"
     tRNA            complement(10069..10142)
                     /locus_tag="ACG8K3_001471"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10106..10108),aa:Ile,seq:cat)
     gene            10329..10652
                     /locus_tag="ACG8K3_001472"
     CDS             10329..10652
                     /locus_tag="ACG8K3_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145351.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10816..11070
                     /locus_tag="ACG8K3_001473"
     CDS             10816..11070
                     /locus_tag="ACG8K3_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145352.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11140..11385
                     /locus_tag="ACG8K3_001474"
     CDS             11140..11385
                     /locus_tag="ACG8K3_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9145353.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11461..11826
                     /locus_tag="ACG8K3_001475"
     CDS             11461..11826
                     /locus_tag="ACG8K3_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP9145354.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11887..12450)
                     /locus_tag="ACG8K3_001476"
     CDS             complement(11887..12450)
                     /locus_tag="ACG8K3_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145355.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(13018..13218)
                     /locus_tag="ACG8K3_001477"
     CDS             complement(13018..13218)
                     /locus_tag="ACG8K3_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9145356.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            13614..13763
                     /locus_tag="ACG8K3_001478"
     CDS             13614..13763
                     /locus_tag="ACG8K3_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145357.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            complement(13970..14683)
                     /locus_tag="ACG8K3_001479"
     CDS             complement(13970..14683)
                     /locus_tag="ACG8K3_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9145358.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(14676..15761)
                     /gene="uxuA"
                     /locus_tag="ACG8K3_001480"
     CDS             complement(14676..15761)
                     /gene="uxuA"
                     /locus_tag="ACG8K3_001480"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9145359.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(15778..16221)
                     /locus_tag="ACG8K3_001481"
     CDS             complement(15778..16221)
                     /locus_tag="ACG8K3_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9145360.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(16255..16608)
                     /locus_tag="ACG8K3_001482"
     CDS             complement(16255..16608)
                     /locus_tag="ACG8K3_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145361.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(16720..17121)
                     /locus_tag="ACG8K3_001483"
     CDS             complement(16720..17121)
                     /locus_tag="ACG8K3_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145362.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(17158..17667)
                     /locus_tag="ACG8K3_001484"
     CDS             complement(17158..17667)
                     /locus_tag="ACG8K3_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9145363.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(17689..18546)
                     /locus_tag="ACG8K3_001485"
     CDS             complement(17689..18546)
                     /locus_tag="ACG8K3_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9145364.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(18564..19397)
                     /locus_tag="ACG8K3_001486"
     CDS             complement(18564..19397)
                     /locus_tag="ACG8K3_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9145365.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(19411..20208)
                     /locus_tag="ACG8K3_001487"
     CDS             complement(19411..20208)
                     /locus_tag="ACG8K3_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9145366.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(20241..20525)
                     /locus_tag="ACG8K3_001488"
     CDS             complement(20241..20525)
                     /locus_tag="ACG8K3_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9145367.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(20522..21523)
                     /locus_tag="ACG8K3_001489"
     CDS             complement(20522..21523)
                     /locus_tag="ACG8K3_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP9145368.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(21525..22427)
                     /gene="gnd"
                     /locus_tag="ACG8K3_001490"
     CDS             complement(21525..22427)
                     /gene="gnd"
                     /locus_tag="ACG8K3_001490"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP9145369.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            22591..23439
                     /locus_tag="ACG8K3_001491"
     CDS             22591..23439
                     /locus_tag="ACG8K3_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9145370.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         6861 a         4741 c         3875 g         8159 t
ORIGIN      
        1 ggtataagtg ccgtatttat tttgttcggc gtactttttt atactcagat cgctacaagc
       61 gaaaaaggat tcaattttga ttggcagacc acattatttg tttctgtcat ttactttgcc
      121 cttctcctag ggatcgttat tgcttatttc ttattgaaac tattgaaaaa aatcgaagat
      181 aaccaagctt tttccactca aagcttaaac atactaaaaa aaatcgaacg aaccattttg
      241 tttattggtt tcgccttttt tggcttactt cctaaattta tgaaagtagc ggatttagac
      301 gatgctccag gtttgatgtt ggtaggaatc gctgtcgtat tcattccttt tgcagtatac
      361 acattaacaa gcgtgctgga aaaactgctt gaacatgcga ttttacttaa gacagatcat
      421 gatttgacag tgtgaggtgc gacatggcaa ttatcgttaa tttagatgta gtattagctc
      481 aaaaaaaaat gagtgtgact gaattgagtg agaaagtggg cattacgatg gcaaacatct
      541 ctattctaaa aaatggaaaa gcaaaagcca tccgtttttc cactctcgaa aaaatctgcc
      601 aagttttgga gtgtcaacca ggtgatttgt tgacctataa aaatgactcg aatactatac
      661 cagaagaata aaataaagta gaacaaacag taaataaaaa ataatctgaa tgatagatgt
      721 gtgagacttt tagaagttat ttacgtctat tattcagatt ttttatttcg attgaatacc
      781 gttttatcta taaataagat atgtactgac tcatacaaag ggggcctttt cttctccatg
      841 tactatcact tttcctgata caactggttt cctgttacgt tgtagttgga tctgctttta
      901 gtataatact ttattcattc caccagactg atagcagttt gagcagtcta aataatcatg
      961 tagtattaca ggccattctg tttcatacct tgaagtgctt aaaacttcag ctaatggccc
     1021 attgtgttaa ctcttactcc attctttttt agtaagctcc catatatact gtaaagcctt
     1081 ctatatatta tgtcatgttg ctttttaaca tttactatgt agttttgata aaatgatttg
     1141 aataacagtg atggacaatt cttttttatt tttaacatta ttttgataat aatgattatt
     1201 tatttaaaaa tacattagat aacatttgtt acgacacgaa aaagcttcac aaacaaatat
     1261 cgttatttta aaatgagggc gaactcacac aattaggagg aatagtatgc catttattgt
     1321 ttactttttt ctttcattac tcgtgatcta tattccatta ccaacatttt tcctgctcgt
     1381 caatcgtcta gcagagcaac atgatacgat tgctgctctt acgaaacttt gtttacttct
     1441 atttgtttgt ctagattact ttacgagcta ttattggcta aaacagacaa gagtaaaaaa
     1501 gagatattat ttcttccttg tctttttcaa tctctttgag tttctgatcc attgcttttt
     1561 acttcatcag tatcatacga tccatttgtg gatcatctgg gttttcaaag tggtcgtatt
     1621 attttgtatg ttcacctttc ctatctcaag aaaatttagg gaggcattgt tttattaaag
     1681 aagcttctct gtatcaataa agacacattt aaggaaaaac ttttaaaaat ctaattggga
     1741 atttgcccgc ataaataaga ggctgtgaca aaagtcttgt cacagcctct tattccgtat
     1801 aaacagtgtt caaaagcttg catcttcgat aatatgccca accaaatgaa aaatatgaga
     1861 agctattttt gtttggctgt tcttattact tgatgaagaa cagctttttc acgacctctt
     1921 tttaaaagtc tttttacagt tttcgagcaa agaatgatac gattagtact aaaatgatgg
     1981 caccaatcaa tgatggcacg attgccattc ctgcaatact tggtccccaa gaacctaata
     2041 atgcttgacc aagtgctgat ccaactaaac ctgctacgac atttgcaata atccccatcg
     2101 aagaaccttt attcgtgatt gctcctgcaa ttgctccgat aattcctccg acgataagtg
     2161 accaaataaa tcccatgttt cgttcctcct aatgttttac ttccttatta ctgaacccgt
     2221 ggttctgtat tttcttttac tttttgtgcg ccgcttccag ctgctgattt tactttatct
     2281 gtttgattcg aagcaaattc accggttttt ttcgctgcgt cagttacttt gtcttgcacc
     2341 gtttcttgat ctttttcaaa ttcttctttt gttttgatgt ccactacgtt agcgttgact
     2401 tcgatcactt ctaaatgtgt catatttttt acttctttgc cgattacttc ttgcatttct
     2461 tcaaagattt tttcgatatc tttgccatat tccacaatga catccatatc aactgctact
     2521 tgttttttac cgacttctgt ttcgatccca gccgttacat tatctgtatt cacaagtttt
     2581 cctgccacat tagagaagaa accaccgttt gcagataata aaccgtctac ttgttcaatt
     2641 gcgattccga tgattttttg cacaacttta tcttcataat tcagctcacc gcgcattgaa
     2701 tcttcctgtt cagaatcatg ctttccttgg ttatttgtat gatcagaacc aagttttcct
     2761 tgtccgtttg tgtggtcaga actaagtttt tcttgtgctt tcgcatggtc agaaggatgt
     2821 ttttgctctt ccacaggttt agaaggatcg attgcatcat gttgttctgg aactttagat
     2881 tctggagttc ctggatgatg aggtgcatgc ttttctgcat catgtttctt ctcttctact
     2941 ccttgttgaa cgttagggac tactggatct ggatgtgcag atactggtgc tgttttgttc
     3001 ttttcatcgt tcacaccatt tgattggttt ggtgtcgttg ggtctggatg tgcagacact
     3061 ggtttttggt tgttcatttt attgttatta tttttgttat tgttattcat ggatgtttcc
     3121 tcctaagatt taaaatagtg atctaagatt ttcgtttgtt tcagataaaa accgatagcg
     3181 acgccgatcg ctgccatgat caagatcaat aaggtcttga aaaagcccac ggtgatccat
     3241 aaaacggcta atatcaatcc aagtacccca aagatgatag ggagcttgta tgtcgaaaat
     3301 agttctttca tttttggtcc ccccctattc tactcgtttg cggtcattgc ttcgtttagc
     3361 tggttcttca aagcctgtgt atttcaccga tactttcact ggctcttttg ttcccaatat
     3421 gtgttgtact tcattttcga tttctttcat caatgttcct gttttaccaa tgagtgctga
     3481 agttcgtttc aactcccctt ttacattgac ggcgatcttg tttttcgttg ctttgacatt
     3541 tacttttggt gaatggatga attcatcttc atgcaagtga gaacgaacca ttccttcaat
     3601 tgctttttta tccaacgtca actccccatc accatgtttc agtagaaagt ttccgattgt
     3661 ttttggataa aacaggaaaa caagtaacgt gataattgtc aaaatagcaa atgcaacggc
     3721 aagccaaaat acatattgtt gcacgtatga attagtgatg gaaaagaatc gaaacttctc
     3781 tagctgaaaa ggcaatggat aggttgcact taatagtatc gcaaacaaaa gcacagatag
     3841 caaaagtaat gttccgatca ctccgatcgt ttttacacct ttattcattt tcgcacctcc
     3901 tcataggggt gaaacgatca acttcttata agttgatcgc ttctccacca gttactccgt
     3961 aaatttgggc tgtaacataa cttgctttat tcgatgctaa gaaaacataa actggtgcta
     4021 attctcctgg ctgacctgca cgttctagta aactttgctg accaaattca gggatcgaat
     4081 cagaaggttg tccttcgtct agttgtaatg gtgtccagat tggaccaggt gctacaccat
     4141 ttacacggat tcctttaggt gcaagctgtt ttgctaaacc gatcgtaaag ttagcgattg
     4201 aagcttttgt tgctgcgtaa tctaataaat gttcgcttgg attgaatgct tgaacagaag
     4261 ttgttgttac gattgctcct cctgctggga tatgcggaac agcttctttc actaaagcaa
     4321 acatagcgat gatgtttatc ataaatgtgt ctttgacttg ctcaagtggt aactcagcaa
     4381 tagactgaca agaaatttgc tgtcctgcgt ttagtaccat cgtatctagc ccgccaagtt
     4441 cactcacggc tttatctatg atttctttag gtgcttgttc gtcacgaaga tcaaaaggta
     4501 gtaagacaat cttacgtcct gcatctttca cgtattttgc tacttcctct gcatcttttt
     4561 cttctcctgg gaagaattga agagcgatat ctgccccttc tctagcaaat gcaatcgctg
     4621 ctgcacgacc gatccctgaa tctccaccag tgattagtac acggcggttt tctagttgat
     4681 tattgccttc ataggattct tctccacaat caggaacagg aatcatattc tgctgtaaag
     4741 ctggtgtatc ctgatcttgt ttaggaaatt tctccgtatg gtacaagttt cgcgggtcga
     4801 ttaattttgg ttctgtcatt gctgtattcc tcctagtttt taaattgtat tgccaaaaca
     4861 aatttctttg tttgatacct gttttaaata gattctttaa tttgtataat ctttccggta
     4921 taaatggtaa cctaaaacta ctgctgctat caaagagcca aagactacga gtataaagcc
     4981 tagagcagaa cttttacccg gtaatcctcc tgtattcatt ccccaaatcc ctgcgaacaa
     5041 cgccggaacg gaaatgatta atgcagctga atctagatat ttcatgatat gatttagatt
     5101 attgtccatc atcccgttga atagatcacc aatactctct aataaatctc gatagatctc
     5161 gatcattttt tctgtttgtc gctgtctcaa atggacatca tagagtagct gttcatcgtc
     5221 taaacgtttg atgaaagttt cagaattcca taattgatcc attgtttccc gttgatctct
     5281 taaggtatga tcgatatata cgatgtctct ttcaagatct gcctgtttat acagttcttc
     5341 attttccgtt gttttacgag ctgctttatc caaagcgtca attcgctttt tcatctctct
     5401 tagttctttc acgtaatggg tgtaaatatc taataatgta tacgtgatga tcttttcaaa
     5461 cgagtttaaa tgatttccat gctttgtcag cagccgatcc acaaattcgg tttttctatc
     5521 gatacaagta atcaagagat tatcggaaag tacaaaagat attggcgtta attttttttc
     5581 gatacgatta tctgactgat ctagattaat cactaccaaa gaaaatggat gctctagttt
     5641 cgtctcataa aggcgctcca tccgagaaac ttcttccgga tattcggttc ctacgaaaat
     5701 atcatcagga agctcgaatt cttcgattgc ttgctcaatc tcttcgggct ttgcactatc
     5761 aagtacgagc caattatatt ttttttcatc tgtttctatt acattctttt cttcatccac
     5821 aaagtagtat ctcaatgtat acctcctttt tggactttct cgaaactatt agctgaagaa
     5881 gcagttcttt aatttgatat tataatcgag tactgcgatt tcattttcta atgaaaagct
     5941 gtcaagctgt ttgttgaagt ccatgttcaa tcgtttaccg aaataatttt gctctaattt
     6001 agatgtatag atgtccaata atggatgctc gattttagtc gcatattctc caaattccga
     6061 aacaaaagga atgatttgaa aaaagctgcc ttttgcaaaa gcacctttgt cccttagaaa
     6121 tggatcggaa tcattgataa gcgcgagtgc gatttttgta ttttccccaa ccttttcaat
     6181 taacgtctct actgcatttt tagacattgt aatttccatt cctactcccc ctcttcagtt
     6241 gttgtcttct attatttaac aactacatct gtttttggtt gaacttttgc atattttgga
     6301 tatttaggga atttagctac acaacggaaa gccgtcatcc ctgcatattt ttcgtaatat
     6361 ttcttcgttg gcccttcaaa atccttgctc cacggtttca catgtcctga aaaatggata
     6421 atgtaaggat tgttgcgagt ttcttcgtat tcacgttcac ctgctgctgt tggatgtttt
     6481 tttgcttttg ccatgatata gccttgtgca ttccaacgcg gatgaagtgg taaccaacgg
     6541 tcatgtaaga tagcatttaa tgcatcttgg tcatggaatc gtaatttttc tggattatgt
     6601 tcgatgaaat ctaatacttt ttgcgtaata ttttcatcta accatttttt cacattgatc
     6661 agcatcaaac ctgagttgaa ataacgcatt gatttagctg aaattttcat tttttccagt
     6721 cgttgatgga acccagcgtc ttcaacagct gctacgattg aatcaccgaa gtcaagacgc
     6781 cataatttag agatatcttg taaagcgatc atgtcgcaat ccatatataa gatacgttca
     6841 acttctgtgc cgcggaacaa ttccggaatc gcaatacgat aataagctgt ttctggaata
     6901 cgatcgctga ttaccacatt tgtaaagaat tctttattga ttttcaagaa ttccacatct
     6961 gagttcattc gtgcgttttt cactgagaag cgtaaaagct gtttactttc aaaatcgata
     7021 tcatcgtcaa tcacatagaa ccgaacaaat ttagacgggt tgcaattatc taatactgag
     7081 acaaataatg cagctaaatg tggtacgaat tttgtgttac aacttgcgac tactgctatt
     7141 tcttttttat tcattgatct cgcctacttt cgttagagta ctgtgtgcta atttttttaa
     7201 gtataaattg gtatacggat gatctttcaa cgtattccaa ggtttatcat gtcctgtaaa
     7261 gtgaacgatc gaaggttttt catttccttc tttatactga cgttcatatt tttcatttgg
     7321 tgctggatga cgttcaaaaa tcagcgaagt ctgcatgttc cattttggat gaagttgttc
     7381 ccaatcttca taaagtacag cattcaaagc atcttggtcg tgatagatga ttcgatcgcc
     7441 attttcactg aggtaatgga tagttttttc tgtaatttct tttttattcc attgatcaat
     7501 gtcaatcacc atcactcctg aattgaagta caagtcatcg gaatcgattc ctaatctttc
     7561 caaagctttc acttggcctg gatcgataat agcgccgatt gtttttccat ttaacggctc
     7621 atcatatagt tttacgatat cgtctagtac taacacatct gaatctaagt aaaggacttt
     7681 cttataatcc tctttagcta ataagtttgg taaagaaatt cttaagtaag cggtagttgt
     7741 gatatggtca ctgactagga agtcttcgta gatatctttt tccactgtca ggaattggat
     7801 acttgcatta cttgaatatt tatttaccgt ttcttccaat ccttctttac tatattctga
     7861 tagaccatcg tcaatcacat aaaattttat gcgtcttgtt ttattgcaat tttctaatgc
     7921 agttgcgatc atcacgctca agtaaggagc ataattttcg tctgaagcag ttaccacagg
     7981 aactacgccg tatctcttct ccatcaaaat cactcctttt tctaatcttt ttctaacctg
     8041 ttctacatct agaatattcc tcatttggtt ttaaagcaaa atatatgctt tgttttcatt
     8101 gaatatttta aaattttttt atcacaaaac gcatcggtaa gcgctaacta ttcgtttttc
     8161 atggcttttt attcaaaggt attgaccaaa aactactata tatagccacc tttttcttaa
     8221 gaatgttttt tgaaaaaatt cataatgaca aagcaagtta gtttcatttt tttaaattct
     8281 catttaatct taaggtaaca ggaaaataga tatgtaaaaa gaaagtagga agataaaatg
     8341 aaactagcag cgattgattt agacggtacc ttgcttgatc gaaatggaaa gataccagaa
     8401 gaaaatatcc aagcacttcg gaaatttgat gaaaatggcg gaatcgtaac cgtcgctacc
     8461 ggaaggaaca gcatttctgc taaagatatt ttttccgaat taggaatcgg tggctatttg
     8521 atttcttcca atggttcact gattgctgaa atgaaggatg gaaaaatggc gaaagttctc
     8581 agacgttcaa aaatagatag cgatacactg agaaaagcct tcttctatgc tcaagaagcg
     8641 aaaatatcca tcattgctag ccgagaaacc caagatgatc agctgacatt cgatgaatca
     8701 aatctagtca aagatgaccc ttattatgaa catttcaatt tacagaacca ttcttttgat
     8761 caaattttaa atatgttgga agatccttct cttagctatc ttaaattagc attaacagat
     8821 aagagtgaag aaaaactaca gaagctacaa agagatctgc aagcagatgg tatccaatca
     8881 gtattctctg atccgcattt cttagaaatc acgcctacag gtatcactaa agctcattca
     8941 cttgtttttc tggcggatta ccttggaatc gaatcgagtg acatcatggc ttttggcgat
     9001 caagaaaacg acattgctat gttgaattac agcgggttga gtattgcgat ggggaatgca
     9061 cagcgacaag taaaagaaat tgcaaaccaa gtaacactga cgaatgaaga agctggtgtt
     9121 gcttatattt taaaaaactt ctattaaaaa gaataaaatg acgatatgaa tgtaggagga
     9181 aagaaaatgt tagttattat tgatatgcaa aatcatattc ttgatccaaa cagcgaattt
     9241 tatattccag agtcagatca attggcagaa cgtattgctc aaagattagc taaagcacgc
     9301 caaaataatg aatatattct cttcacacgg gatataccag ttgaactgaa ggacaaagaa
     9361 gaagaacgag aagatttgca attgatccct cttctttctc cacaagaaaa tgaacgagtg
     9421 attaaaaagt attactttac gattccacct gaaactttag ctgaaatcca ggaaacttta
     9481 tttgaaagca aagaagaaca aaaagagatc gaagtagtcg ggattgaaac aaacttgtgt
     9541 gtactctcta atctgatagg cctgcagagt gcttttcctg aagcagactt ttttgttgat
     9601 ccaatgttag tctccagtag aaaacacgga gaatccgctt tagagctgtt gaaagatttc
     9661 aacgtctcta tcgtcgaatc ataaaaatat ctatcttcat ttacgatatt taacacacaa
     9721 taaaaaaagc acgcagaact gttttagtcc tacgtgcttt ttctatcaaa gtagattggc
     9781 ttttttatga cacagagtga aacggcaggc tcacgacctc tttcttaaat ggtgttcaat
     9841 cagctgaccc tcggtattag cttaacaaac ggcaaaatat gaggaactat ttttccgttt
     9901 gttaagctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa acggtattca
     9961 aaagcttgca tctgcagtaa taagaacagc caagtaaaaa cataagaagc tgtttttact
    10021 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttatg taccttgtag
    10081 gactcgaacc tacgaccgga cggttatgag ccgtctgctc taaccaactg agctaaaggt
    10141 acatcataaa taaaatttat ttttgttact tttcaactat agcacgaatg acaaactctc
    10201 acaagtttca aaatataagt aaaataatat tgttattctt atttctttta cttgtgtcat
    10261 aactattttg ttactttaat ttttatttgc ttattttaga aaaaatgtta ttcttttatt
    10321 tattctcaat gttaggagtg attattttgc catttattgc gattttattc gatttagttg
    10381 cggcagcagc ttattttctc caattaaatt atcaaacctc aggaattctg tttattggaa
    10441 tgatttttca aggtgtgatt acccttttgc ttcttgtcat gatgctcagc tataaaggaa
    10501 aaagatatgc ccgtattcaa acaaaagtat ttgttaaata tgtttccatt cgctatggga
    10561 tcatcattct ttccttttta gtgaatgcga tcgtcctctt tttatatgta ttgaattact
    10621 tagatatcaa tccattgatt ttttctcgat agataagaca cattcagcgg aacagttcct
    10681 gcttatcagc agaaaactgt tctgcttttt catttttcat tgatctattc ttcttttatt
    10741 ttcctagcat ttcatttgtg atgtatgcaa taacatgtta tcttttatta tatgaatcgt
    10801 tgaggagtcc gttacatgat tacgttggtg agagttttgt tttgggtgcc tgcggtcgca
    10861 ctggtcgcaa gtattgttta tcttatgaat tggaacaaag aacggtttta tttagctatt
    10921 ttgactcttc cggctatcta ctttatgtgg aaagttttta attacaacta ctttgaaccc
    10981 gatagtgttt ttatagaaga attatcagga ctggttttgt ctttattgat cgtcattctt
    11041 tatttgatac gtttaaataa aaaacattga gtactattga ttgcatgtgt agctttctat
    11101 agtcattata actattctac tataatgaaa ggtgattaaa tgagtatcga agaagctatt
    11161 ttactggata ctttacgttg tcgtctgaat ttcttaagaa ctggccgatc tttatctcaa
    11221 gtagctgttt ctagcgcgtt aagtattcct ttattcaaat atcgtaagta cgaagaagga
    11281 gaagaaattc caagcatcat tgaattaatc tatatcgctg acttttacga tatttccgtt
    11341 gattacctaa ttggtcgctc agaaacagca agcaaactga agtgattttt tccagccttg
    11401 gtgctggttt tttcatttaa cgtctaaggt taacaatcta tatataaagg agacatttct
    11461 atgcgattat ggcatgaaaa attgatttca aagcttcctc gtcaacagct tttaggacaa
    11521 catcgggaat gctgtgcatt aagaggaaat ggctggggaa agaaacatgc tactgtggat
    11581 tatgtttttc actattctcc ttatagatta ttccaatatc atcagcttgt catggatgaa
    11641 atggaaaaac gtggttacag cccagcacca gagtggaaaa atcctatgta tcgtggcaaa
    11701 tcatgcgaag cattttttga tttacctact gtagagcgca acgtgccgat ttatccggaa
    11761 cacgatgaag tttatctagc agaatgttta gcaaatttgg agcaaaaagg aatttatctt
    11821 acctaatata actagaaaag ccttctatct cctcttactt cattaaaaag ctttaaccat
    11881 cttagatcaa gctaaaatgt cgtaagtttt agggttatct tcatcctcac gtgcaaaact
    11941 aaaagcgtac tctagttttg gttcatcgcc aacttcccta gacataaatg aacatctcct
    12001 gacgtggttt aaaagtaccg catcttcgac gtctgtatac gatgcacagc acagattgct
    12061 tcttttgatc tttcttttga tttttctgtt gcttcttcta caccttcttc gattactaca
    12121 tcgatgctta caattgcttc tgcctaatat tcccatgcat cgtcgattta agcctctgta
    12181 gtcactgtga tcgtttccac attttaactc ctctgtcgtg gcttcagttg attttgtgat
    12241 cagttcagtt gctaagccag agtcagaaca acctgccatt gctagcgtta atattgatag
    12301 taaaaatagt ttttttatca taaggttccc cccctgaaaa tagactaaaa taataacgct
    12361 ttcttttttt gttttcagac gaaaattaat aatttctaaa tattctttaa aaaagcttat
    12421 aactattatg ccttttttaa tatataacac agttattttt aaaaataaga atgctaaatc
    12481 actctacaat aacggcgaaa gcacaaaaaa taaaaaaatt attacgttat taaaaaaaca
    12541 gatgaaagat caggttttat tagatctcat tctttcatca cttgaaggga tttaatgaac
    12601 tactttttcc tctttgtcgt ttttatatat tcgtattcct tcaaacagta ggatagcctc
    12661 tttcaatgct tgatatttga actgtccgtt acttaaaaat ttagatgtgc cgccctcttc
    12721 ctagaacata ttctttttta ctttttcatt aattgacaga ccaatcgaac gaaactgtaa
    12781 atttcgatag gataatgatt ggtcaaactc attcattttc aagacatctc gattcatatt
    12841 caagatagga atctttttat caacatcact ttattcgtat tttcctctac cataaaagct
    12901 tcacgctcca caaaattgtt ttatacacta ctattcagcc cattcaacga ccaatgaatt
    12961 ttttcgtaca aaaaaaccgt cttttctttc gaaaagacgg ttaaaagtca atttttatta
    13021 agctttaaca atgtttgttg cttgagggcc acgttgacca tcttcaacat cgtaagacac
    13081 tgcttggcct tcttctaaag ttttgaagcc gtcaccttgg atagctgaaa aatgtgcaaa
    13141 tacatcattt ccatcttcac cagtgataaa gccaaaacct ttgtctgcgt tgaaccattt
    13201 tactgtaccg ttattcataa ataaattcct cctgatacgt atcaatgtac gttttttcgt
    13261 tgcaataaat atttgatagg caaaaggagt tttatctatg tagatatatc gctcaaatta
    13321 cgtttcaaaa tagattactc atttaatata tcatgctttt tctaataaca caaggataat
    13381 gcaatgaaat ataaaattca ttgtcttcta ttgtcttcga atcgaagaaa aagttgaata
    13441 accgcgattg attcactttt tgttttcttg tttatttgct tttatctgca acttttgact
    13501 ttaaaatttc ttaactatga aaacctttca attggtggta tacttataaa tggaatttcg
    13561 ttatcaacgc gttcgttgcc tttcagcaag tatttaaaga aaggagaatg gctatgacat
    13621 tctataacaa agatatacaa tatcgagtaa ccttggatac agatttgaat ctatttgtcg
    13681 tttttgacaa agaaaatacc aatcatgtag cgacaggtgt tacgatcgaa caagcagtgc
    13741 aagaattaaa aaaatctgca taaggagtgg aaaccctcat atggacgatt ctcgttcttc
    13801 tctttaaacg catctactgg tagataacag tagatgcgtt ttgtgtttgt tctaaattgt
    13861 tcactacaaa taaggtagat acttcaaaat aaaaaattac ttctttttca tctgttccct
    13921 cagtcgtgca ggacaataaa taaagaggac tagaaaatag tcctctttat tagagataca
    13981 accctttact ttctaaaaat cgttttgctt gatgcaggtc ataaaattca tagtctgctt
    14041 tattggattc atataaactt acatctactt tcaacttatg cttcccatta tgatcgggca
    14101 accctatcaa atgatctttg atgagaaaag acaaaatgcc cgctcgcctt gctgcttcga
    14161 tccctgcaat cgagtcttca aatacaagtg cttcacttgg aggaatattc atttttgaac
    14221 aagctttcaa aaagatctct ggttccggct ttcctttttt tacatcatcc cctgaaatga
    14281 tttgatcaaa caaggcatat agattttcca tttttaaata catttctact tttacccgtg
    14341 tattagacga agccacgatt gtatggattc ctctttcttt tgcaaaagat aaaatttctt
    14401 ctgctccttt tttcgtatat actctcttat cttctttcac gattttttct ttatattgat
    14461 tgattgcttc tcgccatttg atatatggaa catcttctcc gtacaaaatg cccatttgtt
    14521 ctcttatcgc catttctgtt tggccagtta gtaaataata gacttcctta cgcatttcat
    14581 aatcatactt ttgtgctgat tttacataag cacgataagc taaacgtcca gtttctaata
    14641 aaaggccatc catatcaaag atgaagagct tgattttatt cataatgctc tcccagtggt
    14701 ttttccttac ttatcgcctc atatagacct tgcatgtaag tgagtcccat tgcacgatca
    14761 tacaggccat atccaggcat agccacttca ccccacacgg ttctaccatg atcagggcgt
    14821 ataggaccgt cgaacccttc ttgcaccaac gctttcataa tatgatacat atcaagggag
    14881 ccttctgtag agggatgtgc cgtttctttg aactttcttt ctcctaaaaa ctccacgttt
    14941 ctgaagtgga caaaatggat gcgcgaacct acctcatgga tgatttcaac taaatcgttt
    15001 tccggatttg cccctaaaga tcctgtgcac agcgttagtc cattatatgg agaatctact
    15061 gcatttagaa ttttcttcaa gtcgccaaga tttttcgtca ctctaggaaa accaaaaagt
    15121 tcccatggcg gatcatctgg atggatagcc atttttacat ctgctttttc gcacactgga
    15181 atcacttttt ctaaaaaata agtcagattt tcaaataatt cttcttcggt cattccttca
    15241 tacagttttt ccagctgagc gaattttttc aaccgttctt cttcccaacc cggcaatttg
    15301 aatccctttg actggctgtg tatcaattga tacatttctc ctggctccat ctgatcgacg
    15361 attttctgat cgtataaaag agaatagctt ccatcgttgt tttcattaaa caaatcagtt
    15421 ttcgcccatc caaaaattgg tttgaagcta taacaaatca atcgaacccc acatcgagac
    15481 aagtttttga ttgttcgtat atagttatca atatagtaat ctcgttcatc tgtccctgcc
    15541 ttgattgcat cgtggatcgc tacactttca attccaagca gttccagtcc ttggctctca
    15601 acagatgctt tcaactcctc aatttctgaa atctcccaaa catctccagg cattttagtc
    15661 agcaaagttc caattatccc atttattccc ggtatttggc gaacatattt taatgggatc
    15721 gaatcccctt tttcaccata ccatctaaat ccccacttca tcttttcagc cttccttcta
    15781 aaaatcgtcc tcatctaatt ttttttcttc cgctgaagca tgccaaacag ttaaattatc
    15841 ttttccttgt ttgacaattt gttccaccgc ttcttctatc ggtgtgtgaa gcatctgatg
    15901 attgattgct tctaacaaca ttggcagatt cacgccccct atgacataag tggctgcttt
    15961 cttttcttca gtcagttcat tgactgccaa gagcgcttga ttgtacggac ttgcgctaat
    16021 tagatcaaca aatactagca tgggttctgc tgtgttgatc atagttaatt tttctttgac
    16081 cttctctccg aatgtttgga tgtcctcccc agactctaag cacattgttt caatatggct
    16141 cgatgtcccg acaattactt ccgccgcatc tttcagtcct tcgctcaact taccatgtgt
    16201 aacgatcatg atttccaaca ttagtcttct cactccttgt tttgcacttt ttacttatcg
    16261 aaaaagagca ataatctgat tggcattttg ccaattaaat gttttccctc gtaaatctgt
    16321 tttggtcaaa tagaagaact gcgcttgctt ttttcttgca ctaaaaatat aattgacgtt
    16381 tttttctttc aacttatcaa cttgtttcag ccactgttct ttatttgaaa aataatttcc
    16441 cgaaaactgt agcggtgtat ctgccccaac agagtctaga taaaaaagaa tagcctcttt
    16501 tttcataccc gatctcacgc tgtctaagcc cttttctcca taacagcctt catcaacaaa
    16561 cgcatacgct acactcggat catcaatcgt acggatcatt tctagcatcg ttaatatgga
    16621 tgaagtattt cgaataaaag tttgtctgct aggccatccg cgagctgctt ttcccaataa
    16681 gaaaaagtag ctcccaaatg ctagtacagt gatacctgtt tatacggata gcagatcgaa
    16741 tacgtgatga ggatttttgt tatatatcat cattccccac caacctaaac tgaacaacaa
    16801 aatgaaggac aataatatag agtagattgt ttttttcctt tggtctttcc aatcaaacat
    16861 gaaataactt cctaatgcat gaacgggtgt atcgtaataa gtacagataa ctttctctgc
    16921 tttttcgata tctccaacat aaatattttt tgatcggtag gctagcgtat cgtaagcaat
    16981 gactgtcaca tctgttctca tactgtaaat atctgatact aatgctgaaa gaaaacgctg
    17041 tttactcttt tttgtacggc gcttacggta aatatagcga taacgaaatt cccaatcacg
    17101 aaacagctcg ttcttcttca ttttttcgcc ctctttatct tttttctaag catttgttta
    17161 tgacaaatca aacttgttca atacatcttt taatcctgtt ttaggtgcag atggagtggc
    17221 ttgaaaataa atattcttta cgccatattg ttcattcatt tctttcaatt tttcagcatc
    17281 tgatttattt aaataaacag atttcgtcac taaaagatct gtatcaggat ttttttgagc
    17341 aatccctcca atattcaatt cttccatggg gattccgtgt tttactaagt cgtaaatcgt
    17401 ttggactgtt tttgcgatca aaatacaatt gtattcttta aattgatatt catcccaata
    17461 gaatttagct ttctctggtg taatgacaat tgttttttgt cctgtacggc ttcctgcatt
    17521 tttgtaaaga tctctcataa atttatcttt tgctacaact tcgtctacaa caaaaatgga
    17581 attcactcca cttttttgag ataacgttac cattacttgc ccatggatca aacgctcatc
    17641 cacacgcgct aaattgacta cacccatctt attcacgttc ccttcttttt ataaaatacc
    17701 gattcctgct agcacaatta atattccagt cagccataat aatgcttgtg tgactttcaa
    17761 tccttttttc gtatagaaca agtacacacc cattacgaca gctaaaggta atatccctgg
    17821 cacgatttga tcaagaattt cctggataac aaactcacgt ccggatatcg caaatttcaa
    17881 tgaagattct acttttacat agtttcccgc aagcaccccc atcataaaca accctaaaac
    17941 tgatagcatt tcaatcgctg tctgaacacc agtgttttgc attaggcctg tagcattacg
    18001 gcccatagtg aaagcttgtc ttgcgaaaaa tacccctagt aaaacctgat acaacccaaa
    18061 gcagataaaa ggaaaaagtg cgcctaaagg acttccctgt tgtgcccaag gtaccgcgat
    18121 tgcgataaaa atatattgga cggttcctga atcgattgca tctccgattc ctgctagtgc
    18181 ccccatcaat cccgctttcg tgttatacat caagtcatcc tgcatcaaga tctctttttc
    18241 ttcatactct tcttctgctc tttgctgttc catagaagac attaggccag taatcacacc
    18301 gccaccccag cttaattgag tgttgaagaa cagtaactgc cgtttatatg ctgcttttaa
    18361 agagtcttca tctttataaa gttttttcaa gattggcatc attccgtata gtagtgatgg
    18421 cgccaagtaa cgttcaaaag agtgaggaat ttcgtttgca aaatgccatc tcagataggc
    18481 ttttgttaca tcttttttcg tgatttcttc aggagcacct gtttgattat ccattatttc
    18541 agccatttcc cattcctcct aaattaaaat ccgtcatcat agtcatcttc gtcatcaaaa
    18601 gatgctgaat tgttattgct tgtggcaggc tgtacttgtt tccctttttg cgactggaca
    18661 aagatcaaag cgataataat cccaaaaatg gcataagtaa ccatcgtaat atctaaagat
    18721 ttcaaaacaa cactcataaa ataagcaagg aagaaaaaga ccagataatc ttttcgaccg
    18781 ataacgtata cagttgtcgc gataccgatt gccgctaacc ctccacctac tacttcaaga
    18841 atgtggataa cgactgttcc ctctaaagct ttgattacat cagcgatcaa tggcgcccct
    18901 aaatataaag caatgaaaac aagcggaacg aataataaaa atgaggcaac cgttggataa
    18961 agaataattg atcgggccat agcttttgta ttcaaatctt ctacagcttt gtctgtatat
    19021 ttacctaaaa aagtattgat gaaaaaacga aactgataca agtaacttcc gagcaatccg
    19081 actggcaccg caacggcaat cgctgcttct ggcgacagat cccctaataa tgcgactggt
    19141 actgcgatag ctgctgcaat agatggttca gctggcattg atcctcctgg agaaaaaacc
    19201 cccatataaa tcatttgcaa agcggctgta acaatcatcg cttgtgcaac ttcccccatg
    19261 atcaacccga cgactaatcc agtcatcaaa ggagaaaaac gtaaggtcaa cgtggctcct
    19321 ccacctaacc agcgggacat aaccgcggct acccataaag caatcaacaa actttgtact
    19381 acacttaata tttccatgtg tcctcctcct tcagatcttt ctgatataac cttccagttc
    19441 catcattgac tgtatcagtg atttctgtgt tacctctata ggaatcaaat gcactttctc
    19501 tttcgaatca ataaattcag ccattttatc tattatctgt ttatctttcg gaaataatcc
    19561 catttgttct agagaaaccg gcagttctaa ttcccgatac aaaggatata actcgtctat
    19621 tttttcccag cgaccttcta gtgccagttg atagaaaatc ccatacgcta ctttttctcc
    19681 atgtagaaaa cgatggcttt ccggaatata tttactgata gcgtcatgca tagcatgtgc
    19741 tgccgcattt cttgcgtatt tatctcctaa tcctccaact aaaccagcaa cagcaaagat
    19801 aatctctgaa agctgtttga actccgatgt cagttttctt tgtgccatat cttggattgc
    19861 tttagcagaa tgattcaaaa tctcttcttt gcagatcttc gcagtatctt gtgctagctt
    19921 caaaaatgat tcatcttgca aaaaatcttg ttctaagatg agctcagatt cataccattt
    19981 tgctagtgta tctgctaacc cggcaatgaa atatggaaca ggggaatcaa tcactaattg
    20041 gggatctgtt ataaaaaaag cagcttgtct gtaaaagtgt tctgttttcc cttctgcttg
    20101 tccattctcc ttatacatga cagcaagagg tgtccatgga gcacaattac tagctaaagt
    20161 agggatgact ccgaaatcga ttcttgcaag attggcacta taacccacta gatcggtcag
    20221 ttttcctccg ccaacgccaa ctacgaaatc gatttcattt ttttgatgat tgtagctatt
    20281 cgtctggctt cagcataact acattctcca gaataacgat gataaaaaaa ttgaatcgtt
    20341 tcgtcattca aaaatactaa tttaggtagc gccttttccc atgataccgt tccgtgggtg
    20401 accagtactc ttttcgcgcc gtatgatttc aagagatcag aaacttttga aataccacct
    20461 tcttcatata aataaaattg agggcctgtt ttcactttta aaccgttcac cattttcttc
    20521 atcatccttt tatatagttg ctctttgtct ggacagaacg ttcaggaaga aggtgctgac
    20581 tgatctgttc gacatctctg acacattttt cacccattgc ttctaaacat tcccttgtgt
    20641 aggccgatat atgtggcgta atcactactt gttcaaacgc taaatacgga tgatcttgac
    20701 gtcccggttc ttcttctaaa acatctgtgg catatcctgc gatctttccg cttttcagac
    20761 cattaatcat cgcttcttca tcaactagag cgcctcttgc actattagat agataaacat
    20821 tttctttcat ctgatcgact tctttatcag aaatcatgtg atagttctct tctgtcaaat
    20881 tcgcacataa acaaatgata tctgattgtt ccagtaattc ttgaagttct actttttttg
    20941 caccaaagct ttcgatgtat aaatctgatt tgtaaggatc ataagctaga acatcacagc
    21001 gaaaaccgtt acgcaaaatt tcgattactc cgcttccggt atttcctgca ccaatcacac
    21061 caactgtttt gttaaacagc gtcctaccga cgaactccgc ccgatcttcc catcgatcgt
    21121 ccttgactct tgcttgtgct tgtgtcgtgc gtcgaagcat ggacaacaaa ttagttatat
    21181 tattttctgc tacagcattt cgttcaacta aagcaggtat gatagatacg attgtctggt
    21241 gttcttttgc tgcttttaaa tcaatattgt tgtaaccaat accatgtcgg gaaatcaata
    21301 gcagttcatc tttgttttca aaaaaatctc gtcgaaaaaa tggcgtaaca cttgcaataa
    21361 tgatattgaa tcctttaagt aactccgcta gttcttttcc tggaatctct tgatcaacag
    21421 taaagaaatg aacgtctcca actgacttta atctttccaa gtggtgtgga aagactcttc
    21481 cgaaactgct ggaattaacg attgctatat gataatctgg cattttactc acccttttca
    21541 actatttcat gtccgccaaa cccatttctt agtgctgaaa ctacttttgc tgaaaaacta
    21601 tcttcttcct gtgaaagatt cctcatgaac aaactagttg caattactgg aactggaaca
    21661 gccatttcta gcgctgtttc aaccgtccat tttgcttcac ctgatgcaga aacgattccc
    21721 cgataattac ttaaattggg atgttcgttt aactgatctt ccatgatttc catcagccag
    21781 ccgcgaatca ccgaaccatg attccaaaca cgtgcgactt gttccaagtc aaaagcatac
    21841 tcactttttt gtacaagttg gaatccttca ccgattgctt gcatcattcc atattcgatt
    21901 ccattatgga tcattttcaa gaagtgtccg cttccggatg caccagcata cagatatcct
    21961 ctttcaattg ctacatcttt gaataattgt tccacggata gaaaagtttc tttatctcct
    22021 ccaatcatta agcaggcttg atgtcgtgct ccttcgattc ctccagaagt cccacaatcc
    22081 aagtaaccga tttcattttt ctgaaactct tctgctcgac ggattgaatc tttataataa
    22141 gagtttccgc cttcaataat aatgtctcct ggtccgagtt ttggaaaaag ttttcttaac
    22201 atgttttcgg ttatctctcc agcaggaagc attaaccaga tgattttttt ctgctcaaaa
    22261 gtagctagaa attcagagat ctcttgataa gcaggaatgt cattttctct tgcctgacta
    22321 acagctgtac ttgaaatgtc gagtccttgt actttgtgat ggtgctcttt taagtttaaa
    22381 gctaaattca atcccatttt tcccaaacca attacaccaa tttccatgat agcgcctcca
    22441 tttcttatac cctcattata caaaaaaatt tttttgtaaa tagattttac aaaaatattt
    22501 tttgtttgat tttttcctac tctccttgta tctttaacat taatgagaac ggaaaaaatg
    22561 ataatcttat tatgaaaatg aggaattctc atgagcgtct ccataagact acaaatcaaa
    22621 acaatgtaca acgacttcag cccaaaagaa caggctatcg ccgattatat tttagaaaat
    22681 ccaagcaaag taagtcatag tcctattagc aatttagcaa gtgaactggg cattgctgac
    22741 tctacttttt ttcaatttac aaaaaaactt ggttataatg gatttaaaga ttttaaaatg
    22801 gccatgttga tccaagaaaa tgatctatct gctatttcta tccacgaaaa tattcagaaa
    22861 acagatactg agctcactat ggcgcaaaag gtgttcgagt ctagtatttc aactttaaat
    22921 gacacaaaaa agttgttaca acaaaaggat ctaaaaaaag cggctactat tatcaatgaa
    22981 tctaaacgtg tctatttttt cggtgttggc ggttcagaga ttgtagcaac tgacgcttat
    23041 cataaatttt tgcgttcccc tatcgcaact tctcacagca cggattacca tattcaattg
    23101 atggaagctt ctttattgac ggaaaaagac tgtgctgttt tgatttctca cacagggcaa
    23161 tcaaaagaaa caatccatat tgctgaaacc gtaagaaaaa ctggagcaaa aacaatcgtt
    23221 attactagcc aagctaattc ttcccttgct aaacttggcg atgtcgtttt tatttctatt
    23281 tctgaagaaa cagaattccg atctgaagct ttagcttccc gtatttctca actaagcatt
    23341 ttggactctc tatatgtgat tttgatgttt tacaacgaaa aagaatcacg tcgttctatc
    23401 gctaaagttc gacatactat ttctgaaatc aaagaatagc accataacag aaaaagcgaa
    23461 aaatccataa aacaaaaaaa gccactacca aatatttggt agtggctttt ttggttctgc
    23521 aagggtggta cagaacatca atagtcggtg ggttagaacg tatgtcatcg catctttgga
    23581 gaaggttgta ttggtattat tcgagttact tgttagatgc gataacaaag ttagta
//
LOCUS       DAZKCN010000043        22704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000043 DAZKCN010000000
VERSION     DAZKCN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.044"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..401
                     /locus_tag="ACG8K3_001492"
     CDS             <1..401
                     /locus_tag="ACG8K3_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9145371.1"
                     /translation="QEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEMHLVD
                     PEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKT
                     AEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            394..1095
                     /locus_tag="ACG8K3_001493"
     CDS             394..1095
                     /locus_tag="ACG8K3_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9145372.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1095..2681
                     /locus_tag="ACG8K3_001494"
     CDS             1095..2681
                     /locus_tag="ACG8K3_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145373.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2700..2921
                     /locus_tag="ACG8K3_001495"
     CDS             2700..2921
                     /locus_tag="ACG8K3_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP9145374.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2918..3679
                     /locus_tag="ACG8K3_001496"
     CDS             2918..3679
                     /locus_tag="ACG8K3_001496"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP9145375.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4059..4568
                     /locus_tag="ACG8K3_001497"
     CDS             4059..4568
                     /locus_tag="ACG8K3_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP9145376.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4643..5182)
                     /locus_tag="ACG8K3_001498"
     CDS             complement(4643..5182)
                     /locus_tag="ACG8K3_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP9145377.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5322..5933)
                     /gene="rpsD"
                     /locus_tag="ACG8K3_001499"
     CDS             complement(5322..5933)
                     /gene="rpsD"
                     /locus_tag="ACG8K3_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9145378.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6195..7046
                     /locus_tag="ACG8K3_001500"
     CDS             6195..7046
                     /locus_tag="ACG8K3_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9145379.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            7074..7709
                     /locus_tag="ACG8K3_001501"
     CDS             7074..7709
                     /locus_tag="ACG8K3_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP9145380.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7729..8502
                     /locus_tag="ACG8K3_001502"
     CDS             7729..8502
                     /locus_tag="ACG8K3_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9145381.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8910..9707
                     /locus_tag="ACG8K3_001503"
     CDS             8910..9707
                     /locus_tag="ACG8K3_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP9145382.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9685..10098
                     /locus_tag="ACG8K3_001504"
     CDS             9685..10098
                     /locus_tag="ACG8K3_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9145383.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10082..12727
                     /gene="mutS"
                     /locus_tag="ACG8K3_001505"
     CDS             10082..12727
                     /gene="mutS"
                     /locus_tag="ACG8K3_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9145384.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12745..14853
                     /gene="mutL"
                     /locus_tag="ACG8K3_001506"
     CDS             12745..14853
                     /gene="mutL"
                     /locus_tag="ACG8K3_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9145385.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14875..15435
                     /locus_tag="ACG8K3_001507"
     CDS             14875..15435
                     /locus_tag="ACG8K3_001507"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP9145386.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15520..17508)
                     /gene="treP"
                     /locus_tag="ACG8K3_001508"
     CDS             complement(15520..17508)
                     /gene="treP"
                     /locus_tag="ACG8K3_001508"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP9145387.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17731..18444
                     /gene="treR"
                     /locus_tag="ACG8K3_001509"
     CDS             17731..18444
                     /gene="treR"
                     /locus_tag="ACG8K3_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP9145388.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18521..18967
                     /gene="msrB"
                     /locus_tag="ACG8K3_001510"
     CDS             18521..18967
                     /gene="msrB"
                     /locus_tag="ACG8K3_001510"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9145389.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19137..19739
                     /gene="ruvA"
                     /locus_tag="ACG8K3_001511"
     CDS             19137..19739
                     /gene="ruvA"
                     /locus_tag="ACG8K3_001511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9145390.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19752..20753
                     /gene="ruvB"
                     /locus_tag="ACG8K3_001512"
     CDS             19752..20753
                     /gene="ruvB"
                     /locus_tag="ACG8K3_001512"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9145391.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20782..21360
                     /locus_tag="ACG8K3_001513"
     CDS             20782..21360
                     /locus_tag="ACG8K3_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9145392.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21412..21894
                     /locus_tag="ACG8K3_001514"
     CDS             21412..21894
                     /locus_tag="ACG8K3_001514"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP9145393.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22011..22385
                     /locus_tag="ACG8K3_001515"
     CDS             22011..22385
                     /locus_tag="ACG8K3_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145394.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7385 a         4067 c         4912 g         6340 t
ORIGIN      
        1 ttcaagagtt tttcattatt attttctttt ctgtattagg agaaggcata cggatttttg
       61 ctcatttacc tattcctgga agtatcttag gtattctttt tttattttta gcatttgaaa
      121 tgcatcttgt tgatcctgaa aaaatcggag caactggtga ttttttattg aataatctag
      181 ccattttatt tgttcctgct ggtgtaggtt tattggaata ttttgatgat attgcttcga
      241 tatggcctgt tttactggga gcagtcgtcg tgtgttccat cgtcacgatg gttgcagcgg
      301 gaaaaacggc agaaggtgta gaagctttgc tgggctatgt ccgtaagaaa aaagcagaaa
      361 gggtatctgt aaacgaaatg gaggagaatc aaattgactg agttttttgt gaatccatta
      421 tttggcttaa gtttgacttt aggagtgtac gtattatctg acaaattttt gcggaaatat
      481 aaactgcctg ttttgaatcc tctcgtatta tcgattgttg ttattatctt atttttgcat
      541 ttttctggga tcagctataa agactataat atcgggggca attttttgac tatgctgatt
      601 actcctgcga cagtggcact tgccatccct ctttataaaa acttccgttt actgaaagag
      661 aatttttatc ctgtaatggc tgcgatttta gcaggaatag tcgcaaattg cttggttagt
      721 gtagggttcg gttacttctt ctctcttcat aaagagatgg tgatctcatt actacctaaa
      781 tctgtaacga cggctatttc tgtcgattta tctcatacga tgggcggggt gaatgccgtg
      841 acacttgcta ttgttgtctc tacaggtatt tttggttcgc tgatcgccac tcatattttc
      901 cgcctattca agatcgaaag tccagttgct cgcggtgtgg cgttaggaag tacttctcat
      961 gctattggta cagcaaaagc aatcgagatt ggtgaaatag aaggaatcat ttccggactt
     1021 gcgatatgcg tcaacggaat attgaccgtt ttattgctac cgttattctt tcagcctttt
     1081 gctggcttgt tttgatgcga aaaccactat tttatgctac aataaccaaa acgttcgagc
     1141 ataaaaaagg agggaaagac gtgtatttgt cttccgagta tcttaaacaa tttgataaag
     1201 atctatatta taagattctc ctcctagaaa gttttgagga tcaagcttgg cataccgctg
     1261 cacaattagc gcaagttgtt cagctggatg cccgcagcgt gtcgaaatac ctgaacgagc
     1321 tgtctaaaaa ctatcagcag tttagcggta agacacaccc tttgtttaca aaaaatcacc
     1381 gttctggtta taacttttat gatacgctcg attcaattga acatgagcgt tttttgatct
     1441 atctcgtgca aagtacgctg aaattccagt tgctgcacga catttttttt gaagaattcc
     1501 atacaatgta ccaatttgcg caaaagcatt acattagtga atctactgct catcgaaaaa
     1561 tcaatgaatg gaaacagcaa ctgcaaactt acggaattcg gctgcaacga ggtacatata
     1621 tcgctcaagg cgaagaagaa atcattcgac tttacttgca tatgacattc tggcagttgt
     1681 ttcgagggaa aatctggcca tttgaaacga tttctcaaat ggacgtaaag aatatggcag
     1741 aacatatcat ggcttttttt aatgttcgtc tgaacgagat caaaaaaaga cgattggaat
     1801 atatgctagg tgcctttttt ttaagaaaat cacagaagca ttatgttgta ttgaatgaga
     1861 aaaaaagacg gctgatttct gacaatctgt tgttccagcg attttgccag gtcatggagc
     1921 cggtatttcc taattatttt caagtagaag acgagttagg cgcattattt cttgtactga
     1981 tgactcgtga agaatattat agtgatccta agatacggaa gaaaattttt gattttcatc
     2041 aacaagcaaa aacaccgccc tttacagccc tttcagaagc aaaagccgct ctttctcttt
     2101 atcaggaaga gcaggggctg ccagcagaaa atctaacctt cgaggcagaa aactatttat
     2161 tcagcagtca ttttttcgcc tatctttttc ctaatgcgaa agaaacgatt gatggcaata
     2221 gtagtgattt tattaatcat ttagttattg aaaacaagga attgaaacaa tggctcgtac
     2281 atttttttga aagtagacac aaacatccca atcatctggc atttaaaaat catgcgtttt
     2341 tgatggggcg gtatctgact gtgtttaaaa cattaggagc ctttactcca cagcttccaa
     2401 agatcacgat ccttttaatg acagatttcc cattatttga agagcaactg ttagaggagg
     2461 gattacggac atttttccgt aatgagtatc agctgatttt tctgccaaca gattatcggg
     2521 ggagagaagt tgatcttctg atttcgacaa gtaaagtcca ccgcaaacca tgggcggatc
     2581 ttgactattt tattgtgact gaagaactaa aattaattga ttacatccaa ctatctcaaa
     2641 aatttgaaat gattcaaaaa caaaaactat taaaacaata acaaagaagg agaaaaaaga
     2701 tgacatttga agaaatttta ccagaattga aaaaaggtgc aaagattatt cgtaaaggat
     2761 ggagtggatt tgaactttat gttgttctcg tatccgatga tgaatttgat ggctgtccgg
     2821 tgacgccgta cttattgatc aaaacaagtg acgaaggttt ttccagcttc gctcctacag
     2881 tttgtgatat tttagcagat gattgggcga tcgtagaatg aaaaaccagt atccagatct
     2941 agctaaacag gttgtattta tcacaggagc tgcttctgga attggtgctg cgcaagcccg
     3001 agcgtttctt gaacaagatg cttttgtatt tggattagat caaaagttcg gtaaaatgga
     3061 agaaatttct ttgcgatttc ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat
     3121 gaaagaactc caacaagcgg tatcgaaatg ccaaaatcat tttggtgaag ttacgatttt
     3181 actgaataca gcggggatat tggatgccta caaacccttg atggaaactg acgaatcgct
     3241 gtgggatctg atttatgaga cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct
     3301 tcctgatatg atccgccaaa aaagcggaac gatcatcaat atggcttcga ttgctggctt
     3361 gattgctggt ggaggtggga tcgcttatac aagcgcgaaa catgcgattg tcggatttac
     3421 caaacaactc gctctggatt acgcaggaca gggtatccga gtaaaaggaa ttgctccagg
     3481 agccatccaa acaccaatga atgcggctga ttttgaaggg aaaggtgaga tggcagagtg
     3541 ggtggcaaat gaaacaccag tcaagcgatg ggcacagccg gaagaagtag ctgagctgac
     3601 gttatttctt gcctctcctc aagcaagcta catacaagga acgatcatcc caatcgacgg
     3661 cggatggttg ttaaaataac cttgtgtttt tttcaagctt ctgtatgata aaactaaaga
     3721 gagaaacaac ggttgatgat aaaaaaagtc gatgactgtt cccgacattc gatgccgaac
     3781 agccagttca aaaaattctt tttaataatc gttaatattc gaaactaagc ggcttttcac
     3841 gacctcttga tataaacgat attcaaaagc ttgcaactgc ggtaacaagt ccagccaaat
     3901 gaaaaatata aggagctatt tttatttgac cattcttatt acttgttgca gggcagcttt
     3961 ttcactacct tctgcgacac ttcagttgga attttcttcc catcctttgg tcgcggtcat
     4021 taccaagata aaattatctt gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt
     4081 cagccaaacg ctggacaacg acagatgtga ctcggatggc gctagtcact ggtttatatg
     4141 tggcagtgac gctggttttg tctgtcttta gctttggagt gattcagatt cgtctctcgg
     4201 agatgttcaa ctatctgtcc ttgtataaca aaagatacat cgtcgcggta acagcgggag
     4261 tagctttggc taatattgct tctcctcttg gactgataga tgttgcagta ggaagcgtca
     4321 gcacgctgct tgtattgctt atcaactata aaatcactag tcgcatcaaa aacatgaaaa
     4381 tcaaaatggc cgtgacagca tgcgtatttg ctttttccat gtttacagta gctggtcagc
     4441 taacgatttt atatcaacta ccttttttct tgaattggtg ggtgattgct ttaggagaat
     4501 tggcatcaat gattgtcggt gggataatta tttattggat cggtaaaaaa gtggatttgt
     4561 caaaataaag atctgtttaa tttaaaatag ggcagcatgg aagagactga atgttgagtc
     4621 ttcttccatg ctgctttttt gattaatttg tatgaagaaa tcgatttagc cgttttttca
     4681 tcaagatacc gatataagct gctccagttg ccttgattgc ttccggcagt aaaaacggca
     4741 gtagtccact ggcaaatgca gttgtaaaag taagatcacc acttattttc agccagatcg
     4801 ttccgcagat caatgcagca agagagccca ttagattggc aaagattgca tataagtaat
     4861 gagcatccgt tctttcgatc atccaaccag ttatccacgt ttgtaagaga aatccaatca
     4921 aatatccgcc agttggccca aataaaacag ctatacctcc agtcatccct gaaaatacag
     4981 gaagaccaat caagccaagt aaaagataaa ctccaacaga tatcaatcct agttttcttc
     5041 cgagaaaagt tgctgttaat ccaatagcaa atgtttgccc tgtcaaagga accaaaccaa
     5101 aaggaatggt catctgagaa aggacagaaa taatcgcggc aaattccgca gcaagtatca
     5161 attctttcaa tgttcggttc ataacgccct cttttcgtta acttaatatt tttatttggt
     5221 taacgaaatt gtaaaacaaa gtgattactt tgtaaataga aaatataaaa aaagaccagc
     5281 tatccaatag atagcggtct ttttcaagaa agcttttgaa attataagct ttggttgtag
     5341 tattcaacga caagagcttc gtcgatttct ggatatagtt cgtcacgttc tggtaaacga
     5401 gtcaatgaac cttccaattt ttcagcgtca aagcttacga atgctgggcg tccaacagtt
     5461 gcttctacag cttcttttac tgttacgatg ttttgtgatt tttcacgaac tgagatcact
     5521 tgacccactt ctacgtggta tgaagggata tcaacgcgtt tgccatcaac taaaatgtga
     5581 ccatggttaa ctaattgacg tgcttgacga cgagtcgttg caagtcctaa acggtaaaca
     5641 acattgtcta aacgttgttc tagtaagatc atgaagttaa caccatgttt accttctttg
     5701 attttgctag cttttacgaa caagttacgg aattgacgtt cgttcattcc gtacatgtga
     5761 cgaagttttt gtttttcagt taattgcatg ccgtattctg attgtttgcc acggctatta
     5821 ggtccatgtt gacctggtgc gtaagggcga cgtgctaatt cttttcctgt gcctgacaat
     5881 gagatgccta agcgacgaga aattttccat gatggtcctg tataacgaga cattaaaatt
     5941 tcctccaatg aatataagtt tttggagtaa aataatccgt tgaaaaattc ataatcatat
     6001 agtttgttct tcaatcttca cctttgcagc cgcggttaca caatcgaacc cgagacgggg
     6061 caacaaacta gggatgagtt attatttttc tgctgcatta ttttacacaa attacagtat
     6121 atgatagatc atccacgaat gtcaagataa ttagtacata aaaacgcttt acaaacctag
     6181 gctgtcttta taaaatgaga gggtatgtgc aaatggaaaa aagcgaagga agtttaagga
     6241 gaaaatcaat gaatccggta tcaccattat ttgagaaaat cgatgcttca attgaaaaga
     6301 aggcgaatct ttttgaaaac agcttttcac gttacgctgt tcgagcggtt ctcgcctgtt
     6361 tgtttttgac tttaggaacg gctgtggcat ttgcgatcgc gatgaagggg gaagatatcg
     6421 cgcatggtct aggaaagatt ttatacgcat ttatgttcag ctggggtctg gtcatgattc
     6481 tatacatgaa cgccgaatta ggaacatcga atatgcttta tatgaccgtg ggtgtttttc
     6541 ggcagagact tgctttaagt aaggcagcca agattctatt tgcctgtatt tttttcaata
     6601 tgattggcgg tatattatgt ggatttctaa tttctatgac tacgccattt caagatttaa
     6661 cgttggacaa ctttatgtta gaatcaatag cagtaaaact agcaaaacct tcagcaacga
     6721 ttttggtgga agggatgttt gcgaatatcg ttgtcaatac cgctgtactg atcagcatgc
     6781 ggatgaaaga ggatgtcgga aaagtgatca cagtcgtatt catcattttc atttttgctt
     6841 ttttaggata tgaacacgtg atagcgaatt ttcctgcctt tacgcttact tactttgctt
     6901 ctcacggaca gatggatgga atgacagtca gcaatgtgtt acataatttg ttttttgctt
     6961 tggcaggaaa ttatattggt ggcggtttag taatgggact gggatatgct tggctgaatc
     7021 agtcaaaatc atcctatgtg gactaaaaag tcgttcatta aaggggaagt gccatggaag
     7081 ttttagtttt tccaaaaaat gaggaaaaag cgttacaaat ggaaaagtac atgaaaaatc
     7141 ttttttcctt tgctggagta cctaagccgg aacgcgctct tttacaacaa ccgttactgg
     7201 atgagagcaa aaatttatct atttctgaag ttttagcttt tgttcattat tattatgaac
     7261 aaatcgaaag agagtatcag tatgtagcga tcgatttagt ccagaaaaac atcaaacggc
     7321 ttacatttga agacttagtc gtattgttgc cactagttca gaaaaaagaa tggtgggaca
     7381 gcatcgactc ttggagaaaa gtatacagca catggtgtaa agcctaccca gagcagcgtg
     7441 aagaagtctt tcaattgttt gaaaaaaagg aagatttttg gctacgtcga atagcaatca
     7501 cgttgcaatt aggttataaa cagcaaacag acacaggact attgatccga gcaatagacg
     7561 aagatcgtca aacaaaggaa ttctttatcc aaaaagcaat tggttgggca ttacgcgatt
     7621 atagtaagac cgatgccgat tgggtaaaaa gacagctgac aacagatttg agtaagttag
     7681 ctgttcgaga ggcaagtaag tatttataag aaagcgagac gacaagagat gagacgcaaa
     7741 ttatttgctt ttgatattga tggaacgttg ttagattcaa acaaacaagc tttagatagc
     7801 acaagagaag cattggaaaa attacgcaaa gccggtcatc tggtcacgat tgctacaggg
     7861 cggagccgat ttcatgcgca agagatcatt cgcgatcttg attttacgaa ctacatttta
     7921 tgtaatggag cggccggttt tttagaccat gaacaagtat ataagaattt actagatcgc
     7981 gaccagttgg atgcgttcgt tcaagaagcg catcatgagc agatcgatac agcttttgtc
     8041 agcttagaca atatcaagcg ttcttcttcc aatgatgtaa cggtgatgga agaagcgatg
     8101 cattctttcg gtgctgtctt gcctgaatta gatcagtttt ttgcagagga acaagaaata
     8161 taccaagcgt tagcattttt cgatcaaaca tatgatggga agttctcttc atatgataaa
     8221 attcgatttg tccgctggca tgagaacagt gtagatgtcg tacctaaaaa tggttctaaa
     8281 gcagcgacaa ttctgcactt agcagaacaa gtaggcatcc ctcatgaaga catcatcagc
     8341 tttggcgatg gacaaaacga ccgtgaaatg ttgcgcatgt caggaattgg gatagctatg
     8401 gggaatgctg tgccagaagt acaagcagaa gctaagatgg tcactgatac aaacgatcag
     8461 gatggaattt ggaaggcatt gaaagagtta agcgtgattt aaaagaggtt gtgaaaaagc
     8521 tgttctgcat caagtaataa gcccagccaa acaaaaatag ctcctcctat ttttcgtttt
     8581 tctgggctta ttaccgcaga tgcaagcttt cgaacaccgt ttaaggaaga ggtcgtgaca
     8641 gaagtggtca gtttcaagaa ataagaaggg atttacgaaa attgcttctc aaatttttgt
     8701 aaaattacgg cttatttctg aagaagtaga tgaaacaacg tttattcggg aaatgggact
     8761 gtgacaagat gtttgtcata gtcccatttt tttattttat ttttcaaaac taagaaatac
     8821 caggaaagag ttccttttgt ttgacctttt ctttcttatt gaaaacaact ataatataga
     8881 gtgatgttaa aagagaagag gaatttagtg tgcgggtatt gtttattggt gatgtagtag
     8941 gttcaatggg acgagagatg attactgaat atcttcctag attgaagaag aagtaccgtc
     9001 cgcaagtaac gattgtaaat ggtgagaatg ccgcttctgg acgtggaatc acagaaaaaa
     9061 tctataaaaa atttttgcaa gacggcgtag atgtcgtgac gatggggaat catacgtggg
     9121 acaaccgcgg aatctttgag tttgtaaatg aagctaaaaa gatggttcgt ccggcaaatt
     9181 ttccggaagg gacgcctgga caagggatgg tctttgtcaa agtgaaccaa gtcgaactgg
     9241 cagtgatcaa catgcaggct cgttctttta tggtggactt ggatgatcct tttcgtaaaa
     9301 tgaatgagct ggtagaagaa gccagaaaac gtacgccgat catttttgta gattttcatg
     9361 gagaaacaac aagtgaaaaa caagcaatgg gctggttttt agatgggaaa gtttcagcag
     9421 tggtcggtac acatacacac gttcagacaa atgatgcgcg aattttgcca agaggaacag
     9481 cgtatctgac agacgttggg atgacaggtc cctatgatgg tattttgggt atgcgcagag
     9541 aacctgtaat cgaaaagttt ctgactgcat taccaaaacg ttttgaagtt gtggaacaag
     9601 gccgatcgat tttaagcggc tgtatcttgg aattggatga tacgacaggt catgcaaaag
     9661 aaatccagct gattcagatc aacgatgatc gaccgttcat ggagtagaaa gagaggcagt
     9721 tttttgcagc aggagccgga aattcaaaaa gaagtaagga aactgacaaa acttttaaga
     9781 gagaatgaaa cgatcattcg ctacaaagaa cttgaagaaa agatccagca aaaccagtat
     9841 ttagctgagt tgagagaaaa aatcaaacaa gcgcaaaaag atgctgttca ttttgcccac
     9901 tatgacaaac cagcagcaga aaaagaagca atcaaacaag cggatcaatt catgcaagaa
     9961 tttgatcagc atccgcttgt tgtagcttat agaaaacagc tgttggaagc agatgatctg
    10021 cttcatcatt taacaactat gatacaagaa gaaatcaatg gacaaataga ggaggaaaaa
    10081 catgcctcaa aaaactaaaa atactcctat gatggaacaa tatctaagca tcaaggccca
    10141 ataccaagat gcttttttgt tctatcgttt aggagacttt tatgaactat tttacgaaga
    10201 tgcgatcaaa gcgtcacagc ttttggaatt aacgttgacg agcagaaatc gaaatgcaga
    10261 agaacctatt cctatgtgcg gtgtacctca ccatgcagca caaggatata tcgatgcctt
    10321 gattgaaaaa ggctataaag tagcgatttg cgagcaggta gaagatccaa agacgactaa
    10381 agggatggtc aagcgcgaag tgatccagct agtcactcct ggtacagtca tggacagcaa
    10441 aggattgtcg gcaaaagaca acaatttttt aacagctgta gttagtcaag gaaatgaatt
    10501 tggctttgct tatgccgatt taagtacggg agaattgaag acggctcggt tgcatgatga
    10561 agaagctgtt ttaaatgaag cctctgcatt gcagacaaaa gaactcgtat taggcagtga
    10621 gatcactgat acccttcgtg aacagctaag cgggcgttta gggctagtct tttctcaaca
    10681 aaatgagatg gaagaaaacg cggaatttag ctttttgaca agcgaactag tcgatccgct
    10741 ggaaaaagaa gcaaccggca aactattgac ttatttatcg gtgacacaaa agcggagttt
    10801 agctcatatc caaaaagcag tagaatacca accagatcat tttttgaaaa tggattatta
    10861 ttcaaaattc aacctagaat taagccaatc tattcggact gggaaaaagc acggcactct
    10921 tctatggttg ttagatgaaa caaaaacagc aatgggtggc cgtttactaa aacaatggct
    10981 agaccgtccg cttatccagg caaaacaaat taaagcaaga caagaaatgg tcgcttcttt
    11041 gctagacagc tattttgaac gtatcgattt gcagtcagca ttaacgaaag tctatgatct
    11101 ggaacgccta gcaggtcgag tagctttcgg taatgtcaat gggcgagact tgatccagct
    11161 gaaaacttct ttagaacaag tgccaatgat cagagggttg attgaaggaa tcgatcaagg
    11221 tcaatggcaa agtctgcttt cagaaatgca gccgatggac catctcgtcc aattgatcga
    11281 ccaagctatc caagatgatc cgccgttaca aataacggaa ggaaacatca tcaaagatgg
    11341 attcaatgag cagctcgata tgtaccgttc tgctatgaga aacggaaaga aatggttggc
    11401 agaacttgaa gcaaaagagc gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa
    11461 ccgcgttttc ggttattata tagaaattac aaaagcaaat cttggaaatg cagaactgga
    11521 gaagtatgaa cgaaaacaaa ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa
    11581 agaattggag acacaaatcc tcgaagcaga agaaaaatct gttgatttag aatatcagtt
    11641 gtttttagct gtgagagaag aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa
    11701 agcaatcagt gcagtagatg tgctacaaag ttttgcaacg atcagcgaac gctatcagta
    11761 tgttcggcca gaacttgtca gtgacaagca tcagcttctg atcaaagacg gacgacatcc
    11821 tgtagtagaa aaagtattag gacatcagga atatatccct aacagcgtag aaatggcaga
    11881 agacgaaatg atcttgctga ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg
    11941 acagttggca ttaactgttc tgatggcgca aatgggctgt ttcgttccgg caaaacaagc
    12001 agttttacct atttttgatc ggatttttac ccgaattggt gcaagtgacg acttaattgc
    12061 tggacaaagt acttttatgg tagagatgat ggaagccaat caagccctgc gttatgctac
    12121 gccaaacagt ttgattttgt ttgacgaact agggcgagga acagcaacct atgacggaat
    12181 ggcgttggcc caagcaatca tcgaatatat ccatcgtcat gtacaagcaa aaacactctt
    12241 ttctactcat tatcatgaac tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt
    12301 ccatgttgga gccgttgaaa aagacggaga agttgtcttc ttgcataaaa tgatggatgg
    12361 acctgcggat aaaagctacg gcatacatgt ggcaaagatc gcaggattgc cttccaatct
    12421 actagaacga gcagcagata tcctgcatac gctagaaaat ggcgataacg gacaccatac
    12481 tgttccagct gaagtagaag agaaaactaa aacagccaaa caggagcaaa taaaaccacc
    12541 tgcagaaaaa gtaaatcaag accatataca agaccgtgta aaagaagaaa accaacagct
    12601 ttctttattc agtgaattat ccgaaaatga aacagaagtc attgaatcac tgaagcaaat
    12661 aaatttacta gaaatgacgc caatggatgc actaaacaca ttatatgaat tacaaaaaca
    12721 actgtaggaa gaaggcggat atcgatggcc aaaatccaag aattatctga acgtttagct
    12781 aatcagatcg ctgcgggaga agtagtggag cgaccagctt ccgttgtgaa agaattagtg
    12841 gaaaatgcaa ttgacgccgg cagtactcag attgatatct tagttgaaga agcgggactg
    12901 cggacgatcc aagtgattga taacggagaa ggcattttgg cagacgacgt agagaatgcc
    12961 ttcaagcggc atgcgacaag taagatccat aatcgcgacg atctttttcg catacgaacg
    13021 ctgggctttc gaggggaagc tttgccaagt attgcctctg tttctgaaat gactctggaa
    13081 acggcgaatc aggaagaaaa acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt
    13141 gagcatcgac cagcaccact gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc
    13201 aatacgccag cccgcttgaa atacgtgaaa acactgcaca cagaattagc gaacatcggg
    13261 gacatcgtca atcgactggc attgagtcat ccatcgattg cttttcgttt agtacatgat
    13321 ggaaacaaaa tgctggcaac tgctggaaat ggcgatctga aacaaacaat tgcgggaatc
    13381 tacggattat ctaccgcaaa aaaaatgctg aaaatcgaag gaaaagatct agattttgag
    13441 ttatttggct atgtttcatt gcctgaggtg actcgtgcca gtaggaatta cttatcgacg
    13501 atcatcaatg gacgttttat caagaacttt tccttgaata aagcaatcgt tgcaggttat
    13561 gggtcaaaat taatggtcgg tcgtttccct attgctgtat tggaaatcaa aatggacccg
    13621 cttttagtag atgtaaatgt tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa
    13681 gaactaacag agctaattag taacgccatt caaaaagcat tgtctcaaga aaatctgatt
    13741 ccaagtgcag cggataatct acggtttaag aaaaaagtac ctgatcagcc taaaacagaa
    13801 caaatcgaaa tcgatttgtc ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt
    13861 ttgaattttg atcgagagac aggtaccttt ttcgtagaag aactaaagaa cacatttgtt
    13921 gacagaattg ttgataagtc tgtggaaaat gttgaaaact attctggttt ttctgaagaa
    13981 aacaccactt atccagaaaa aacagcgaaa acggtggata agtttggtga aaatgttgat
    14041 aactttgtag aaaaaacagt ggaaaaatct gaaaagaaag aatttcccat tgtggataac
    14101 atggagaaaa caaatgaaga acaagcagat aaatccgtag aagaggatga aaagatttac
    14161 gaggaagaat tgacactcca tcctgaattt gatttcactc aaggaaacga caaaacatta
    14221 gaacaagcca tcaagaaatt agaaacggaa aaaccagtac aacgttttcc tcagttggaa
    14281 tattttggac agatgcacgg aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc
    14341 attgatcagc atgctgccca agagcgcatt aagtatgaat atttccggga aaaaattggg
    14401 gaagtatctg atgatctgca agaattgctt gttccatttg tgttagacta tccaaacagt
    14461 gatgctttga aattaaaaga gcaaaaacac ttgctagaag atgcagggat ttatttagaa
    14521 gaatttggtc aaaacagctt tattgtacgg gcccatccta cttggtatcc caatggagaa
    14581 gaagaatcga ttattcggga gatgatcgat atgctgctga caactggaag tgtgagtgta
    14641 aaaaaattcc gtgaagcaac agcaatcatg atgagttgca aacgatcgat taaagccaac
    14701 cattatttga atgaagcaca agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat
    14761 cctttcaatt gtccccatgg ccgtccagta ttgattcact ttaccaattc ggatatggaa
    14821 aggatgttca aacgaatcca agacccgcat taagtcaaag agaagaggga aaacatgaag
    14881 attgtattag catcccaatc accaagaaga aaagagttat tggctcatat cgtttcagaa
    14941 ttcgagattg aacctgctga tatcgatgaa acgccatttc cagacgaaca gcccgtcgat
    15001 tatgttcgta gaatggctga agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa
    15061 gaccatttag tgattgccag tgacacgact gttgttctca atcaagagat catgggtaaa
    15121 ccagaagatc tggctgaggc tgaattcatg ctgaaaaaac taagcgggga aacacatact
    15181 gtatatacag ctgttgtttt aaaaacaaag gaacgagaag aacggatact agcagaagca
    15241 catgtcactt tttatccttt gacagacgaa gagatcaaac gctacttgga gacgggggat
    15301 tatgcggata aggcaggggc atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa
    15361 atttctggag attattacag tatcgtagga tttccgattg gtgctgtcaa tcaggcactg
    15421 aaaacattca tttaaacaaa aaagcggact atctgatttt cgattgaaaa tccatgatag
    15481 cccgtttttt ttattgaaaa gattacttat ctactctgac taagatttta cttcagcttt
    15541 accgactgct tcgcccgggc gaacaagtcc tgattggtca agagaaagtc ctgcaacttt
    15601 ttcattattg gtcaaagcaa caataatgtc agttttcttg ccagcttgtt ctatttctgg
    15661 caaatccatt tcagcaatca gggtctctgg tgtgacaagg tatccttctt tcacgaatac
    15721 attgaaagga acacccttta gttcaacagt atctaatccc atgtgaacaa gtacttccag
    15781 cccctcatta gaaagaatac caatggcatg ttttgtttca aagacactcg tcactttacc
    15841 gtttactggt gcatagactt tgccgtttga cggttcgaca gcataaccgt cacccatcat
    15901 tttttgtgag aaaactgggt catttacttc agttatttcc ttgatggtcc ctgcagcgac
    15961 tgcgaataat gtttcttttg ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga
    16021 ctgtggcgtt gctgttgcgc catttgctgt ttgaagttgt acatcagcag cattttcagg
    16081 aacagcagga tcaatcttat tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa
    16141 tggaatgatg acagcaatga tcattgcgat gatgaaaggt acccatgttt ctgcttggat
    16201 agctagtatt cctggcaaac cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt
    16261 agcaaacatg ccagctaatc cagagccgac cattgcagcg acgaaaggat agacgtattt
    16321 caaattgatt ccgaacatag caggttcagt tacgcctaag taacaagaaa tcattgctgg
    16381 aatcgagatt tgttcttctt ttttattccc acgatgtaag aagatgatgg ctaagactgc
    16441 agagccttgg gcgatatttg ataaagcaat catcggccat aaattcgttg atccgaaatc
    16501 tgcaatcagc tgcatatcga tggcattggt catatggtgg agaccagtaa tcactaaagg
    16561 agcatacagg aatccgaata cggcactaaa caaccagctg atagaagacg tcaatccagt
    16621 atttacgata tttgagatcc agcttccgac tgtccagccg ataggaccta aaataacgtg
    16681 tgcagctaaa acagttggta acaatgaaaa caatggaaca aaaatcatcg agatcgactg
    16741 agggatatat ttgcggaaga agatttctag atacgctaac ataaaccctg ccagcatggc
    16801 tggaataact tgcgcttgat aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa
    16861 tggaatatct gctgcggctg tggaagctac actataagca tttaataatt gcggagaaac
    16921 taacgtaatt cctaaaacga ttcctaatat ttgtgtagta cccatctttt ttgcaatact
    16981 ccaagtgata cctactggaa gaaaatggaa aattgcttca cccggtaacc acaaaaaggc
    17041 attgacacca ttccaaaact tcgagtgttc aacgattgtt ccaccaagac tttcaaactg
    17101 gataccttca agtacatttc tgaatccaag gatcaatcca ccgacaatga ttgcaggaat
    17161 cagtggtgta aagatttctg ctaaaacggc aatagcacgt tgaacgggat gaaggttttg
    17221 ttttgcagct gcttttcctt gttctttgga aaccccttca actccagata ctgcaacgaa
    17281 gtcgttataa aaagtagaaa catcgtttcc tatgatgact tggaactgtc ctgcattcgt
    17341 gaacatccct tttacgctag gtatatcttc gatcgctttt tcgtccgctt ttcctggatc
    17401 attcaggaca aaacgcatac gagtcgcaca atgactaact gcagcaatat tttcttttcc
    17461 gccgatatca tgtaacagct tctcggcatc tgcttgatat tttgccatat caataaaccc
    17521 ctttctctta acttgtctat gcaagttgtt tcttcattta ctatactttt tttcggagag
    17581 aattgcaagc gttttattaa aaagtacgag agaaaatgat atcgttttaa aaagcttaaa
    17641 ataagctttt cttaaaaata ggaacattga aatacaaaaa gagctgtagt atagtgaaga
    17701 aatgatggaa gaacgagtga gggaaaaaac atgaataagt tcaatgaaat ttttttagat
    17761 ttggaacaga aaattttagc gaaagaatat cctccgcatt cgcttttacc cagtgagaac
    17821 caattgatca aaatttatgg tgtttcacga gaaacaatta gaaaagcgct gaatcttttg
    17881 actacggaag gttatattca aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat
    17941 cgcttcgact ttcctatttc tggattgact agctataaag agctacagga gacccaacgt
    18001 attccaagtg aaacgatcat ccatacgtta gaagaaacag aagtgacaaa agcattgaac
    18061 gagatcactg gctgggagat cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc
    18121 aatggcgaag tagttatatt agacaccgat tatctattaa aggaaatcgt tcctatttta
    18181 actccagcac aagcacaagg atcgatctat gaatattttg aaaatgagct ttctttgacg
    18241 attgcttacg cgcaaaagga gatcacagta gaagaagtaa cggaaatcat gaaaacagcg
    18301 atggatcttc acgatgacac ttatgcagta gtagtcagaa gcctcgttca tctggaagat
    18361 acccgctgtt tcgagtattc tgagtcggtt caccgattag ataaattccg tttcgttgag
    18421 tttgcccgac gccgaaaagt gtaaatagtt caaaagaaag caaattgttg gcatctgtcc
    18481 actttggcgc tatgatagga tcacaaaggg ggcattttta atggataaac caataagacc
    18541 agaaaaagaa gaactgaaag aaaagttatc acccatggct tatgcagtca cacaagaaaa
    18601 tgcaacagaa cgaccattta gcgggaaata tgatgatttt tacgaaaagg ggatctatgt
    18661 agatatcgtc agcggtgaac cgttattcag ctctgcagaa aaatatgatg caggttgtgg
    18721 ctggccatca ttcagcaaac caataacgaa aagacaagtg cgcgaaaaag ccgatttttc
    18781 tcatggcatg catcgaatag aagttcgaag caaagaagca gattctcatc tcggacatgt
    18841 gtttaccgat ggaccagtag atcaaggtgg tctacgttac tgcatcaatt ctgcctcact
    18901 gaaattcatt ccatatgaaa agatggatga attaggttat ggggaattca aatcacttgt
    18961 agaataatgg aaatacacaa agagcggtac aatacgggga tttactcgat cgtccgctct
    19021 ttttttattc aagcgattgc tatttgaaag aaacgctccc tagactcctt ttttcacaag
    19081 gtgaaatgtg ctacaataaa gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt
    19141 atgaatatct gacaggtgtg gttaccttta tcaaccccta ttacatagta gtagaaacca
    19201 atggtatcgg ctaccagatc gcactaggaa atccttaccg ctacagtagc aaactaaatc
    19261 aaaaaataaa aatttatgtc catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat
    19321 ttgattcgct agaagaaaag caactgtttt tgcgattagt aagtgtttca ggaatcggtc
    19381 cgaaaagtgc ccttgcaatc atggcaagcg aagaccactc cggtttgatt caagcagtag
    19441 aatccagtga tgtcacttat ttgacgaaat ttccaggagt agggaaaaag acagcacaac
    19501 aaatgatctt ggatctaaaa ggaaaattcg gtgagctgag tatcgataca ccttttagtc
    19561 tatttgatga agcaaacaca aaagacgcta cagctctatc tgaagcaatg gaagctctct
    19621 cggctcttgg ttacagtgat cgagaaatca aacgtgtaga aaaacaatta aaagaagtag
    19681 ataatcagac aacggatgag tatctacgcc aagcactcaa gctaatgatg aaaaaataag
    19741 gaggagacca aatgacagat gaatacttgt tatcaccaga atctggcgaa aatgaactga
    19801 gtttggaaaa atctcttcga ccacagctgc tttctcaata tatcggacag agaaaagtca
    19861 aacaagaact tcagatttac atcgaagctg ccagaaatcg ggaagaacct ttagatcatg
    19921 ttctgttgta cgggcctcct ggattaggaa aaacgacgat ggccatggtc attgctaatg
    19981 agatgaccgt aaatattcgt acgacgagtg gccctgctat cgaacgtccg ggtgacttag
    20041 tagcaatctt gaatgaactg gaagcagggg atgtcctgtt tatcgatgag atccatcgat
    20101 tacctagagt ggtagaagag atgctttatt cagctatgga agatttctat gtagatatca
    20161 tggtaggaca agggccaacc gcacatccgg ttcatttccc tcttcctccc tttactttga
    20221 ttggcgcgac gacacgggca ggtatgcttt ctgcaccttt acgagatcga tttggcatca
    20281 tttctcatat ggaatattat gacgaacagg atctgcagga gatcgtttta cgttcttcgg
    20341 atatttttca aacagagatc attgaagaag gggcattgga aattgctcgc cgatcacgag
    20401 gaacgcctag aatcgctaat cgcttgctca aacgggttcg tgattttgcg caagtccaag
    20461 gagacgggaa aatcgatcgt cctatagcgg atcaggcact tactttgcta caggtcgatc
    20521 aggctggatt ggattatgtt gaccagaaat tgttgcgaac aatgatcgag ttatatggcg
    20581 gcggaccagt agggttaagt acgctttcag taaacatagg agaagagcgt gagacggtag
    20641 aagatatgta tgaaccgtat ctgatccaaa aaggattttt aaagcgtaca cctagagggc
    20701 gaattgctac tgcttatgcc tatgaacatt ttggttacga ctatcatata tgatttttgc
    20761 ttttagattg gaggaaaatg aatgagtgat acaacacgta cgaaacaatt gatcaaaaac
    20821 gtattgattg atttgtgcca aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca
    20881 aacgaagcaa aaatcaatcg acaaacattt tattatcatt ttacagataa atacgatttg
    20941 cttcgttggg tctattgtga ggatgcattg cattatttag atccacaatg tctttcactt
    21001 gataattggg aagagcaagc attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt
    21061 tatgcaaata ctgtgctttc tgacccagaa attttacaaa aagagttttc tcaactgatc
    21121 caaaaatcat ttatcaagat ttttgaacaa atggatgaag aaagacaact ttctgcaaga
    21181 gacaaactat tttatgctcg ttttttttct tacggttgca gtggtgtatt agtcaattgg
    21241 atcacgaatg aatatgaaga aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc
    21301 aaggatacgg aattatattc ttatcgtctt tatgcaaaag aagagttgga agaacagtaa
    21361 tagaaaaatt ttttgttgga aaagcaataa tcgctaaaag gagatgggga aatgacaaaa
    21421 attttatttg tttgtttggg aaatatttgc cgttcaccga tggcagaagg cttgctgaga
    21481 aaacgaatag cattagaagg aagggaagca gaattttttg tggattctgc tgctacaagc
    21541 acgtatgaag ttggaaagac accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa
    21601 gtagatatga cgaatatggt tgctcggcaa atcacacccc acgattttga aacttttgat
    21661 tggatcattg gcatggatca agaaaatgta gaagaactga aacgaagagc accacgctca
    21721 gcgcagggga aaatccattt gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt
    21781 cccgacccat actataccaa taactttgat gagacatatc acttgatcac agaaggcctg
    21841 aatcattggt ggaatatttg gattgatgaa aataaaataa caaataattt gtaaagttaa
    21901 cgctttcttt ttaaggcgat gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa
    21961 aataaaatta attaagtaac actttcttag ctaaaggagg agtgcattcg atgaacgaag
    22021 agacagtatc gccaatgaag aagcttacga agttagttac tcatacttcg tatcaacttt
    22081 ttgattcttt tgtaaatgac actgaagaac atgctaagaa tgtacgattg caaggaaagt
    22141 ttattcttga gctgcaaaaa gctgctactg aacatagtct agttatctta caagtaagag
    22201 caaatcgttt gagtagcaag tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg
    22261 aaaaagaccg agtgatgatc cgcctacaaa acgaagagca gcagatccgg atggttcctg
    22321 tagaacaaat tgaaaaaata acaatccttt caccagcagg ggaacaggaa cggttatcaa
    22381 aataatttat accagataga aaagcacgta tgagcttgta ccatcaatca ttactaatca
    22441 aaaataaccg aatgctttaa gctgatatca gtaaaacgga acgttttagg atgagtatca
    22501 tagcatgaca gcattcggtt gattagttga atcgattgga tggtacaagt ttttttgata
    22561 aaaaaaggtc ctgctagccg gggagctagc aggataagga gttaaaaatg aaaaagtgtt
    22621 gttagggttg tttgttggta tgcttatata atagtcatta gttgtgaata ttttgtgacg
    22681 aaagttttcg aaagttgtta agtt
//
LOCUS       DAZKCN010000044        22683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000044 DAZKCN010000000
VERSION     DAZKCN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.045"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG8K3_001516"
     CDS             131..3658
                     /locus_tag="ACG8K3_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP9145395.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG8K3_001517"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG8K3_001517"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP9145396.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG8K3_001518"
     CDS             7446..7787
                     /locus_tag="ACG8K3_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9145397.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8K3_001519"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8K3_001519"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9145398.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8K3_001520"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8K3_001520"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9145399.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8K3_001521"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8K3_001521"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9145400.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACG8K3_001522"
     CDS             12998..13735
                     /locus_tag="ACG8K3_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9145401.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACG8K3_001523"
     CDS             13749..14564
                     /locus_tag="ACG8K3_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9145402.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACG8K3_001524"
     CDS             14577..15224
                     /locus_tag="ACG8K3_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9145403.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACG8K3_001525"
     CDS             15240..15896
                     /locus_tag="ACG8K3_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9145404.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACG8K3_001526"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACG8K3_001526"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9145405.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACG8K3_001527"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACG8K3_001527"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9145406.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACG8K3_001528"
     CDS             18238..18756
                     /locus_tag="ACG8K3_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9145407.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8K3_001529"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8K3_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP9145408.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="ACG8K3_001530"
     CDS             complement(19643..20563)
                     /locus_tag="ACG8K3_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP9145409.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20689..21741
                     /locus_tag="ACG8K3_001531"
     CDS             20689..21741
                     /locus_tag="ACG8K3_001531"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP9145410.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACG8K3_001532"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACG8K3_001532"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9145411.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7560 a         3953 c         4828 g         6342 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tga
//
LOCUS       DAZKCN010000045        21845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000045 DAZKCN010000000
VERSION     DAZKCN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.046"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..2483)
                     /locus_tag="ACG8K3_001533"
     CDS             complement(102..2483)
                     /locus_tag="ACG8K3_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP9145412.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGAGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2473..3120)
                     /gene="recU"
                     /locus_tag="ACG8K3_001534"
     CDS             complement(2473..3120)
                     /gene="recU"
                     /locus_tag="ACG8K3_001534"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP9145413.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3184..3723
                     /locus_tag="ACG8K3_001535"
     CDS             3184..3723
                     /locus_tag="ACG8K3_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP9145414.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3813..4232
                     /gene="gpsB"
                     /locus_tag="ACG8K3_001536"
     CDS             3813..4232
                     /gene="gpsB"
                     /locus_tag="ACG8K3_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP9145415.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4254..4621
                     /gene="rnpB"
                     /locus_tag="ACG8K3_001537"
     ncRNA           4254..4621
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8K3_001537"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4830..5996
                     /locus_tag="ACG8K3_001538"
     CDS             4830..5996
                     /locus_tag="ACG8K3_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP9145416.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6042..7538
                     /locus_tag="ACG8K3_001539"
     CDS             6042..7538
                     /locus_tag="ACG8K3_001539"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP9145417.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7619..8302
                     /locus_tag="ACG8K3_001540"
     CDS             7619..8302
                     /locus_tag="ACG8K3_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9145418.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8947..10137
                     /locus_tag="ACG8K3_001541"
     CDS             8947..10137
                     /locus_tag="ACG8K3_001541"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9145419.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10239..11192
                     /locus_tag="ACG8K3_001542"
     CDS             10239..11192
                     /locus_tag="ACG8K3_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9145420.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11261..12610
                     /locus_tag="ACG8K3_001543"
     CDS             11261..12610
                     /locus_tag="ACG8K3_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9145421.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12793..13116)
                     /locus_tag="ACG8K3_001544"
     CDS             complement(12793..13116)
                     /locus_tag="ACG8K3_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145422.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13245..14408
                     /locus_tag="ACG8K3_001545"
     CDS             13245..14408
                     /locus_tag="ACG8K3_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9145423.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14478..15827
                     /locus_tag="ACG8K3_001546"
     CDS             14478..15827
                     /locus_tag="ACG8K3_001546"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9145424.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15872..16303)
                     /locus_tag="ACG8K3_001547"
     CDS             complement(15872..16303)
                     /locus_tag="ACG8K3_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9145425.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16691..19333
                     /gene="alaS"
                     /locus_tag="ACG8K3_001548"
     CDS             16691..19333
                     /gene="alaS"
                     /locus_tag="ACG8K3_001548"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9145426.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19533..20129
                     /locus_tag="ACG8K3_001549"
     CDS             19533..20129
                     /locus_tag="ACG8K3_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9145427.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20251..21399
                     /locus_tag="ACG8K3_001550"
     CDS             20251..21399
                     /locus_tag="ACG8K3_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9145428.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21437..>21845)
                     /locus_tag="ACG8K3_001551"
     CDS             complement(21437..>21845)
                     /locus_tag="ACG8K3_001551"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP9145429.1"
                     /translation="KVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQAEF
                     EVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSEFS
                     LLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         7031 a         3855 c         4615 g         6344 t
ORIGIN      
        1 aaaaatcatg taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac
       61 acaaaaaaga aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct
      121 gaggtgctgc tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca
      181 ctggaagaag caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa
      241 ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac
      301 tcagcagttg attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt
      361 gagcttccag gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca
      421 ttcgatctag ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg
      481 cttggcatct cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga
      541 taaacatcag aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa
      601 cctgtccaca cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac
      661 accccagaag aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc
      721 ttaccggctt gatataaccc agcgatttgc gcattcgtac cagctccttc agtgatcgta
      781 tcttttaaaa tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct
      841 ggctcataag tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg
      901 taataagtcc ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct
      961 ccgtattttg tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc
     1021 gtactgtatt cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca
     1081 ttgaataatt tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg
     1141 gtgcccatat attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac
     1201 ggtgcgtcag taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa
     1261 tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg
     1321 cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta
     1381 ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga
     1441 tccgtgttga cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc
     1501 tctaaaccgt ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct
     1561 ttgacataat tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca
     1621 tccgttaccg gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga
     1681 agcatcgtat acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga
     1741 tcataggcgg aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt
     1801 tctgataatt tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt
     1861 gacatataca ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc
     1921 aatcttacag ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg
     1981 gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg
     2041 ccagaggtaa agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt
     2101 ttataaaaac ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca
     2161 ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt
     2221 gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct
     2281 tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt
     2341 attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa
     2401 cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag
     2461 cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata
     2521 gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc
     2581 tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca
     2641 gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa
     2701 caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc
     2761 atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa
     2821 gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc
     2881 cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg
     2941 ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc
     3001 ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat
     3061 tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat
     3121 ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct
     3181 gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt
     3241 tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat
     3301 cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta
     3361 tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt
     3421 ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa
     3481 ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct
     3541 agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc
     3601 tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa
     3661 gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa
     3721 taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg
     3781 ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag
     3841 acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg
     3901 aattcttaga caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac
     3961 aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag
     4021 ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata
     4081 tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag
     4141 caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct
     4201 tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg
     4261 ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg
     4321 cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg
     4381 aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc
     4441 ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat
     4501 ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata
     4561 gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga
     4621 aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa
     4681 aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact
     4741 tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc
     4801 tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg
     4861 caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg
     4921 aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag
     4981 ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt
     5041 atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac
     5101 tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg
     5161 attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc
     5221 cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta gctttgctga
     5281 aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc
     5341 gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga
     5401 caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta
     5461 ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg
     5521 aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa
     5581 aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg
     5641 aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac
     5701 aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt
     5761 acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg
     5821 tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg
     5881 aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg
     5941 atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt
     6001 actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt
     6061 tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata
     6121 cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt
     6181 acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt
     6241 ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag
     6301 agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt
     6361 cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac
     6421 acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg
     6481 aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc
     6541 ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag
     6601 aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga
     6661 agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag
     6721 atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac
     6781 gatggaacga aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg
     6841 gtatgtatga acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat
     6901 caatgggtat ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca
     6961 gcttctggaa aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata
     7021 ttccttttga caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg
     7081 aggcagacag tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga
     7141 ttttcaatga aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag
     7201 agtatctggg aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt
     7261 caggtgcaag ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat
     7321 tacttcattc tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt
     7381 ctccaatcaa aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa
     7441 atcaattaat catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata
     7501 tgaaagacct gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct
     7561 gaaattcatc taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat
     7621 ggataaatta caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg
     7681 acatgggagc gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga
     7741 accgcaggca gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga
     7801 taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt
     7861 gccaatagta gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc
     7921 taaaaatctc tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga
     7981 tcgtatcgaa gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca
     8041 tgggcacccg atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta
     8101 ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa
     8161 aatgaatact gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt
     8221 cttagaagaa ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg
     8281 gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt
     8341 cggatcattt tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg
     8401 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat
     8461 attgtaactt gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg
     8521 tgacaagact tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata
     8581 ttcagagagt aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat
     8641 tggtctatgc aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt
     8701 ctatcaatgg ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa
     8761 aggtaattgt tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat
     8821 gtggttaaat aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt
     8881 gtagatgaat gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg
     8941 gagacaatgt cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa
     9001 gaaaaggggt tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc
     9061 gtagacggaa aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc
     9121 gaagaattga aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca
     9181 gtccgaacga tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa
     9241 ttcaaaggga cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct
     9301 atctctgcag taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct
     9361 attattgacg gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg
     9421 aaagaccttg agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg
     9481 tatatcacag atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt
     9541 ccgattgttg aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta
     9601 actggaaaag gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa
     9661 atgggcacgt tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca
     9721 ctaatcgact ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca
     9781 ggtgcagctg cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc
     9841 gaaaaagttt gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac
     9901 atcggtatgt ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag
     9961 actttctcta agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact
    10021 gtgcctcttg ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg
    10081 cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta
    10141 aatttagttt tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca
    10201 tctgaagata gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag
    10261 gatgtttagg acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg
    10321 aaaatgtcat tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg
    10381 agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg
    10441 acacattgat tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag
    10501 cctggaattt gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc
    10561 tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata
    10621 ccccacaaga tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag
    10681 aactactatg tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc
    10741 ctggattgat ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata
    10801 tttattattc agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa
    10861 tggatatgat gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc
    10921 ctgatcgatt ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag
    10981 agatcaaagc atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc
    11041 ctgtacgtca gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag
    11101 aggaatgggg cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa
    11161 aactgaaaga aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt
    11221 atacttttgg gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac
    11281 gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc
    11341 tttatcgact gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt
    11401 agcagaagtt acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat
    11461 ttctgtctgt gcaggactaa caggagctga actggcagct gctattccag aaacaggagg
    11521 gatgattaaa tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc
    11581 gcaagtcgtg atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca
    11641 gttcgtcaat ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc
    11701 tgtatcgatc ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat
    11761 tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca
    11821 agaaggagta gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc
    11881 agccttggga gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg
    11941 aaatatttcg ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg
    12001 aatcatcgga atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc
    12061 tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg
    12121 cggcttcggt ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa
    12181 tggctatacc cttactggaa tgcgtctccc ttatgttatt gcaaaagaaa acaacctgcc
    12241 ttttagcaag ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat
    12301 cttagagcta gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga
    12361 tatgctgatc tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct
    12421 tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc
    12481 actagtagca atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt
    12541 tttagcttcg atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa
    12601 acgtagatag ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata
    12661 ttttatttaa atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac
    12721 gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc
    12781 gatttctgtc ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat
    12841 atttaataaa accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta
    12901 gccaagcact ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg
    12961 ccaagtctga agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa
    13021 ataatgaatt gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa
    13081 aataggtacg ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc
    13141 ttactttact ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag
    13201 gactgctaag ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg
    13261 aaccgattcg ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt
    13321 tttttattta tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt
    13381 attctttcgt tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa
    13441 aggagacggt tgcccaaatg acagggccaa agatggagct gcatttattt gcttcatggc
    13501 aacatttcag agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa
    13561 tgaagcagag tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca
    13621 tttatttaag atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc
    13681 tttctctatt ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat
    13741 accgcttatt tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg
    13801 tcgttacgcc gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt
    13861 atgaaaaagg aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga
    13921 catttttatc tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag
    13981 gggcagttac ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg
    14041 taccgtttat aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg
    14101 aagaagaccg cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact
    14161 ttctttttta tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa
    14221 cgaatacggt gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag
    14281 gtatttttat tttaaatatc ttgctatcat ttttgagaaa aaaacggatt ctgttttatg
    14341 aaaaagctag tcacacatat actgttagca cgattggcca aacaaaggat gatgattctc
    14401 gaaattagct gagcaaaaac gctttgtctg tgttataatg ccaaagtcgt gataatttaa
    14461 agagaagagg tgcacgaatg aataattttg atatattccg cttcaaaccg tttatcaatg
    14521 aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt ccaggaacgt ttgattcctt
    14581 taattgcaaa aggaaaaaat atcattgggc aatctcaaac tggtacagga aaaacacata
    14641 cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa agaggaagta caagttgtta
    14701 ttacagctcc aagtagggaa ctagctgcac agatctatga agcagctaca caaatcgcta
    14761 aattttctac accggagatc cgtgtctcga attttgtcgg cggtacagac aaacagcgtc
    14821 aattaaatcg tctgcatcac caacagccac aggttgtagt aggcacgcct ggtagaatat
    14881 tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc atttgcattt gttgtcgatg
    14941 aagcggatat gacattggat atgggttttt taaatgaagt agaccaaatt gcaagtcgat
    15001 taccagaaaa gctgcagttt ttagtttttt ctgccactat tccagaaaaa cttcgtccat
    15061 tcttacgaaa atacatggaa aatccaatca ttgaagaaat caaaccaaaa gcagttattt
    15121 ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa agatgagaat caaatgattt
    15181 atcaactgct gacaattggt catccttatt tagcaatcgt atttgccaat acaaaacagc
    15241 atgtggatga aattgccgat tacttgaaaa accaaggctt gaaagtagca aagattcatg
    15301 gagacatcac cccaagagag agaaaacgag tgatgcgaca agtacagaat ctggagtatc
    15361 aatatgttgt agcgactgat ttagctgcta gagggatcga tatcgaaggt gtctctcatg
    15421 ttattaatgc agaaattcca gaagatctag acttctttat ccatcgtgtc ggacgtactg
    15481 gacgtaatgg cttaaaagga acggccatta cgttatattc gccaaatgat gagcaagcca
    15541 ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa agaaatcaaa aacggggaaa
    15601 tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga aaaatcaaaa gatgtattag
    15661 atcccacgct gattggtctt gtgaagaaga aaaagaaaaa gatcaagcct ggttataaga
    15721 aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga acgcaaaatc gaacgcagac
    15781 agcaaagccg tactgctcgt aaaaataaga aaaattcaca taaataaaaa acaagaggtt
    15841 gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag actttttttc attccgatac
    15901 aaagtacttc atcttcaata aaaggttctg tgcaagtatg atacccttgt ttttcataaa
    15961 aatctacagc cgtcatttca gctactacat aactagtctg acaaccttct gaaaccgccc
    16021 gtttttcagc agcttcaagc aactgaccgc ctaacccttt tcctcggtat tcttttgcta
    16081 cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt ttcataacgc aaagtagcta
    16141 ctggtctttt ttgatcaaga aatagataat aggtttgttg cgggatatct tttccgtcga
    16201 attctagttc tgcaggaatc ccttgttcta atacaaaaac ttgatatctc aaaaagcagc
    16261 tagctgcgcg tatccattca tccgctccaa ctattttttt catcatattc ctccttgtca
    16321 tgaatagcag agatgtattg attcctttat tcttatcgta ccaaaaatcg tggagtatga
    16381 ccaaaaggaa atgaaaataa aaattgagat attgacagtg atcaatagaa tgcgtatact
    16441 aattaagtac gaaaaggata tgatttgttt tttaagactc acagaaaatt ctttcttggc
    16501 tgaaaaaaga atagtcacta atcaaattgc tccctttttc tattttggta gaaataccga
    16561 cgcagctcag cgttatcgag tttaagaggt aatgattaca catcattgca atcaaggtgg
    16621 taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt gtatttaatt tatagaaagc
    16681 aggtaatgac atgaaacaat taagtagtgc acaagttcga caaatgtttc ttgatttctt
    16741 ccaatctaaa ggacatacga ttgaacctag tgcgtctttg atccccgtga acgatccaac
    16801 cttattgtgg atcaactctg gtgttgcaac attgaaaaaa tattttgatg gttcagttgt
    16861 gccagaaaat ccaagaatca caaatgccca aaaatctatt cgaacaaatg atatcgaaaa
    16921 tgtcggtaaa acagcacggc accatacgat gtttgagatg ctgggtaatt tttcgattgg
    16981 tgactatttc aaaaatgaag ccatccactg ggcatgggaa tttttgactg atccaaaatg
    17041 gatggcattc gatccagaaa aactttatgt aacagtttat ccaaaagata cagaagcaaa
    17101 acgcatctgg catgaagaag ttggcctgcc actagatcac attattgatg tagaagacaa
    17161 cttctgggat atcggtgcag gcccatgtgg tccggatacg gaaattttct atgaccgtgg
    17221 agaagcttat aacgatttag cagaagacga tcctgaaaac tatccaggag gagaaaatga
    17281 acgttattta gaaatctgga acttagtatt ttcagaattc aaccataagc ctgataacac
    17341 atttgaacca cttcctcata aaaacattga tacggggatg ggattggaac gcatggtctc
    17401 aatcgttcaa gatgcgtcaa cgaactttga gacagacttg tttatgccta ttatccacgc
    17461 cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac ccagtaacag atatttctta
    17521 caaagtaatc gcagatcata ttcgcgccct ttcatttgct attggcgacg gtgcattgcc
    17581 atcgaatgaa gggagagggt atgtcttacg ccgtttgctt cgtcgtgctg tcatgcatgg
    17641 gaaaaaatta ggtattgaac aagcattctt atatcgttta gtccctgttg ttggggaaat
    17701 catggtaagt tattatcctg aagtacttca acaaaaagaa tttattgaaa aagttatccg
    17761 tacagaagaa gaacgtttca atgaaacaat caatgaaggt ttggaaatct taaatcaagt
    17821 gatccaagaa gtaaaaaacg aaaacgggac gactctagca ggaaaagaca tcttcaaact
    17881 atacgacaca tatggcttcc cggtagaatt aacggaagag gtggcagaag atgaaggact
    17941 gaaagttgat catgctggtt ttgaaaaaga aatggatgca caacgcgaac gtgctcgctc
    18001 tgcccgcagt actgaaaaat caatgggggt acaatctgct ttattgacag atatcaaagt
    18061 agaaagtaaa ttcattggat acacagaaca agaatcagat agtcaattat tggtcatcgt
    18121 taaaggtgag tctcttttga atgaagtcgc agaaggtgaa gctcaaatga tctttgatca
    18181 aacacctttc tatgcagaaa tgggtgggca agtagcggat caaggatgga tcatgaatga
    18241 agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa gcgcctaatg gtcaatttat
    18301 gcacaccgta gaagtaaaag caccaatgag tgaaggacaa acctatcatc tattgatcga
    18361 tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca acccatctac tgcacaaggc
    18421 attgaaagaa gtacttggcg agcatgcaaa tcaagctggt tctttagtcg ctcctggaca
    18481 tttacgattc gactttaccc actttggtca aatcacttca gatgaattag ctgaaatgga
    18541 aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt gaaacaattg aaacagatat
    18601 cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt gaaaaatacg gacatgacgt
    18661 tcgagtagta aatattggcg gctggtcaat cgaactgtgc ggaggcacac atgtagcaaa
    18721 tacagaaaac atcggaatct tcaaaatcgt ttctgaatca ggaattggtg caggcgtgcg
    18781 acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta cttgaagaag aagaaaaaca
    18841 actaaaacaa gtagcaacaa ttgttaaatc acctcaattg aaagaagttg tttcaaaagc
    18901 ggaacaattg caacagcaag taagagagct acaaaaggaa aatgaacaat tagctagcaa
    18961 gttagcgaat caacaagctg gtgatgtatt caaagatcca gaaacgatca atggaaaaac
    19021 aatcattgca gcacaagtaa aagtgaaaga tatgaatcag ctgcgtcaat tagctgacca
    19081 atggaaacaa aaaaatattt cagatattct tgttttagca aacgaacaag aaggaaaagt
    19141 cagtctgcta gcggcaatga cgaaagaatc aaatgaagct ggactaaaag ctggcgactt
    19201 gattaaagca atcgcaccga aagtaggcgg tggcggtggt ggacgtccag acatggcaca
    19261 agctggcggg aaaaatcctg ctggtattcc agatgcactt aaagctgcga aagaatgggt
    19321 tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa agttcaaaca ttgaacgaaa
    19381 catgattatg ggggcgaaga caaacaagtc ttgccctcat acttttttca attggatggc
    19441 gcatctaatc aaatggtcgg attcggatgg tctttagttg acgaaagtga cagaaatttt
    19501 tacgattagt ataaataaaa aggggtgaaa aaatgaatag tagaaataag acctttcgac
    19561 tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca agcgatggtt ccttggctgg
    19621 ggtttattcc attaggattc attagtttaa caattatcca tatcacagta attattgctg
    19681 cagtagtatt aggcccaaaa gatggcatgg tgattggctt gttttgggga atcgctacaa
    19741 ttgttcgtgc ctatgccatg ccaacaacgc catttgatac attagtgttc acgaatccga
    19801 ttatctcggt agttcccaga gtacttgttg gcttagtagc aggcttggtt ttccactgga
    19861 tttatcaaaa aaataaatca ataacgatca gctcagcctt tgcaggggta ctgggttcat
    19921 tggtcaatac agtcttagtg ttaggattca tgggattatt ttatacaggt gcaacggcag
    19981 atgcctatgg tgtagactct gctcttttat ttaaaacctt agcaggggtt gcagcaatca
    20041 atgggatacc agaagcaata ggtgcgggaa tcataacacc attactagca aaagcgttat
    20101 ttgcagcaac accgttgaaa ccagaataat gaccatcttt taatagtaat aggacgcacg
    20161 atagatagaa aaaggaagag aggagaggaa aacaatcgtc ctctccatct ttttacctga
    20221 gcaacaagca aactagaagg ggtaaaaaac ttggctaaga aacaagaaga aaaaaagaaa
    20281 caaataccgc aacagcggac aaaaaaagga gtactagcgg gtatcttagg attggttacg
    20341 aatctgatct tgtttgtctc taagctaatg atcgggcttt tttcaggaag tgtctccatt
    20401 atggctgatg cagtgaacag tctatcagat actgcttcat ccatattgac tttgatcgga
    20461 tttaaaattg ctgcaaagcc agcagatcag gaacatcctt atggacacga acgcttcgaa
    20521 tatatcagtg ggttatttgt ttcgatcatc attacttatg taggatttca atttttagag
    20581 tcttctgctg aaaaaatcat ccatccagaa aatgtctctt taacacccat cgtctttttt
    20641 gtcctaatca tttccatctt tttgaaattt gcacaaggca aaatgtatca ggttattgcg
    20701 caatccattg attctgaaac attgagagct acagcaacag atagttataa tgatgtcttt
    20761 actacactgg ctgtattgct ttcagcagca atcgaatggg taactggatg gcgtattgat
    20821 ggatatatag gttttgcttt agcattatat atcctttata gtggattgac gatgcttcga
    20881 aattttgtat acgaactatt aggaagtcgc ccaacgccag cagagattga agaaatggaa
    20941 gagcatttaa atgcttgcga tgatatactt ggctatcatg atttattggt gcataactat
    21001 ggacccaata aacgctttgc atcagtccac atcgaagtag atgaatcaat gaatttgaat
    21061 gtggctcata agattattga tctgatagaa aaagacttta aaaagtcttt ggatgtggaa
    21121 cttgtctgtc acttggatcc aatgcctgtt gataacgaaa aatactataa tatcttgcgg
    21181 cagttaaagc aaattcttct tgaaacaggt acagggctta atattcatga ttttagagta
    21241 attcatcaag gaaaaacgct gcaatttgat gtagtggtcc ctgagaaatt ccagtttgtt
    21301 gatacagaac tagaaagaat cttacgtagt caaatagaag aaaaaatcgg agattaccaa
    21361 ttggaaatta cgtttgatca caattatctt ttatattaaa tgagaaacta taaaaagact
    21421 ccttaaagtt tttaatttaa agagtctttt tttcttttaa ttgtttatgg agagcctgac
    21481 gtgcaagatt atctgctcct ttatttttgt tttcgggcaa ccattgcagg atcaataaag
    21541 aaaattcgga tagaagttct tggattcttt gaaggtaggg aaggaagagc tcgttcttcg
    21601 ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc tgaaaaacag aaaatcgttt
    21661 cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt taaaaagact tcgaactctg
    21721 cttgatggtt tgtccctata gaaagcggga tagatagctg ttcttgttta ccttgataga
    21781 caatcaagat tccacctcca ctaggaccag ggttgccttt tgtagaggca tcaatgtata
    21841 ctttt
//
LOCUS       DAZKCN010000046        21594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000046 DAZKCN010000000
VERSION     DAZKCN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.047"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="ACG8K3_001552"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="ACG8K3_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP9145430.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="ACG8K3_001553"
     CDS             complement(2019..2921)
                     /locus_tag="ACG8K3_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9145431.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="ACG8K3_001554"
     CDS             complement(2918..3610)
                     /locus_tag="ACG8K3_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP9145432.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="ACG8K3_001555"
     CDS             complement(3653..4294)
                     /locus_tag="ACG8K3_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP9145433.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="ACG8K3_001556"
     CDS             complement(4332..4646)
                     /locus_tag="ACG8K3_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145434.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACG8K3_001557"
     CDS             complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACG8K3_001557"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9145435.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACG8K3_001558"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACG8K3_001558"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9145436.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="ACG8K3_001559"
     CDS             complement(5983..6915)
                     /locus_tag="ACG8K3_001559"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP9145437.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="ACG8K3_001560"
     CDS             complement(6912..7688)
                     /locus_tag="ACG8K3_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP9145438.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACG8K3_001561"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACG8K3_001561"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9145439.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /locus_tag="ACG8K3_001562"
     CDS             complement(10932..12008)
                     /locus_tag="ACG8K3_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP9145440.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="ACG8K3_001563"
     CDS             complement(12013..13293)
                     /locus_tag="ACG8K3_001563"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9145441.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="ACG8K3_001564"
     CDS             complement(13312..14238)
                     /locus_tag="ACG8K3_001564"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP9145442.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="ACG8K3_001565"
     CDS             complement(14251..15534)
                     /locus_tag="ACG8K3_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP9145443.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACG8K3_001566"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACG8K3_001566"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP9145444.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="ACG8K3_001567"
     CDS             complement(16322..17233)
                     /locus_tag="ACG8K3_001567"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP9145445.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACG8K3_001568"
     CDS             complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACG8K3_001568"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9145446.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="ACG8K3_001569"
     CDS             complement(17699..18397)
                     /locus_tag="ACG8K3_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP9145447.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="ACG8K3_001570"
     CDS             complement(18384..18770)
                     /locus_tag="ACG8K3_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145448.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="ACG8K3_001571"
     CDS             complement(18917..20587)
                     /locus_tag="ACG8K3_001571"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP9145449.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="ACG8K3_001572"
     CDS             20770..21003
                     /locus_tag="ACG8K3_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9145450.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="ACG8K3_001573"
     CDS             complement(21102..21533)
                     /locus_tag="ACG8K3_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP9145451.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
BASE COUNT         6073 a         4836 c         3820 g         6865 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacga tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaa
//
LOCUS       DAZKCN010000047        20324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000047 DAZKCN010000000
VERSION     DAZKCN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.048"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..974)
                     /locus_tag="ACG8K3_001574"
     CDS             complement(60..974)
                     /locus_tag="ACG8K3_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9145452.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(989..1990)
                     /locus_tag="ACG8K3_001575"
     CDS             complement(989..1990)
                     /locus_tag="ACG8K3_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP9145453.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(1987..4170)
                     /locus_tag="ACG8K3_001576"
     CDS             complement(1987..4170)
                     /locus_tag="ACG8K3_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9145454.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4173..6620)
                     /locus_tag="ACG8K3_001577"
     CDS             complement(4173..6620)
                     /locus_tag="ACG8K3_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9145455.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6604..6996)
                     /locus_tag="ACG8K3_001578"
     CDS             complement(6604..6996)
                     /locus_tag="ACG8K3_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9145456.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7083..7580)
                     /locus_tag="ACG8K3_001579"
     CDS             complement(7083..7580)
                     /locus_tag="ACG8K3_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9145457.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(7697..7921)
                     /locus_tag="ACG8K3_001580"
     CDS             complement(7697..7921)
                     /locus_tag="ACG8K3_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145458.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8025..9935)
                     /gene="ltrA"
                     /locus_tag="ACG8K3_001581"
     CDS             complement(8025..9935)
                     /gene="ltrA"
                     /locus_tag="ACG8K3_001581"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9145459.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(10719..11924)
                     /gene="mobT"
                     /locus_tag="ACG8K3_001582"
     CDS             complement(10719..11924)
                     /gene="mobT"
                     /locus_tag="ACG8K3_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9145460.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(11947..12100)
                     /locus_tag="ACG8K3_001583"
                     /pseudo
     CDS             complement(11947..12100)
                     /locus_tag="ACG8K3_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12103..13488)
                     /locus_tag="ACG8K3_001584"
     CDS             complement(12103..13488)
                     /locus_tag="ACG8K3_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9145461.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13517..13903)
                     /locus_tag="ACG8K3_001585"
     CDS             complement(13517..13903)
                     /locus_tag="ACG8K3_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9145462.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(13919..14233)
                     /locus_tag="ACG8K3_001586"
     CDS             complement(13919..14233)
                     /locus_tag="ACG8K3_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9145463.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14586..15065)
                     /gene="rlmH"
                     /locus_tag="ACG8K3_001587"
     CDS             complement(14586..15065)
                     /gene="rlmH"
                     /locus_tag="ACG8K3_001587"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9145464.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15493..16782
                     /locus_tag="ACG8K3_001588"
     CDS             15493..16782
                     /locus_tag="ACG8K3_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP9145465.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(16821..17426)
                     /gene="ytpR"
                     /locus_tag="ACG8K3_001589"
     CDS             complement(16821..17426)
                     /gene="ytpR"
                     /locus_tag="ACG8K3_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP9145466.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17622..18086)
                     /locus_tag="ACG8K3_001590"
     CDS             complement(17622..18086)
                     /locus_tag="ACG8K3_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9145467.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18108..18428)
                     /locus_tag="ACG8K3_001591"
     CDS             complement(18108..18428)
                     /locus_tag="ACG8K3_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP9145468.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18571..19650)
                     /gene="pepA"
                     /locus_tag="ACG8K3_001592"
     CDS             complement(18571..19650)
                     /gene="pepA"
                     /locus_tag="ACG8K3_001592"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP9145469.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            19850..20200
                     /locus_tag="ACG8K3_001593"
     CDS             19850..20200
                     /locus_tag="ACG8K3_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP9145470.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
BASE COUNT         5668 a         4240 c         3745 g         6671 t
ORIGIN      
        1 attttttatc aaagagagta cgtgattaca aaatagctgt aataatgtac caatatttgt
       61 tattctataa tcttccaatt actcccgttc ttttcaagta ccaaatcaaa ttgagatacc
      121 tgcgttgctt tggtctgctg gtcgatatac tccactgtca gcgataccgt gacttgatta
      181 tccttacgat tgtaaatagg atttaccagt tcttgaaaga tgtactcttt tccgattggt
      241 tttaatatcc cgtcattcac atagtaggaa agttcactgg ctgtcgctgt aggatagagc
      301 ttgaagaacg tcgttaaaaa ctcattgatt tcattggttg taatggaatc aaccgtaccc
      361 tcactttcaa tagcttttgg tttatagtct gatttctttg gtatgctagt aatggtcgga
      421 ttcttaatga ggaccatatt tccagtactg tctacataga cactcactat ataagcagag
      481 tggacggtct ttgtattttc cccctctgta atgagttggt ctacactgta ggttacatca
      541 aattgattgt cgccatctgc ttctaccgtc catatctgaa atccttttac agaagacgat
      601 acaggaatat ctttacgaac tgtatcgaca ttcagagctt gaagttcctc tgtcagatat
      661 gctttcaggt tttctatgcg attatcaatg gacttgtcgt ttagttccca tgaatagtag
      721 actttcgcaa agttctctac aaaattttcc acatgatgag tatcgacata ttccttttct
      781 atgatggtgg tttcatgaat ggtatgggta tcaatggctg taaagtgctt gaatatcgca
      841 aagctgaaac taagtcctaa aagtacccat aggacaatca caaccttttt acgaggattg
      901 accttataga cacgaggttt cttttccttt ggtgtctgtt tttctgtatt ctgatttttt
      961 ccgaatttca tcattgaatc gtcctttctt attgtttgat tcgtcctgct cccactaaat
     1021 gctgttgcca gtaagagctt gttaagtcgg cataaccgat agggtcgcct gcatgaaaca
     1081 tacggttatc gcctaaataa atcccgatat gagtaatata agagccagcg ttataggtag
     1141 aatgaaagaa aaccaaatcg ccggcttttg cttccaataa tgggatatgc cgggttacat
     1201 catattgctg ttgtgctgtt cgtggtaagt tgattccagc ttttccatac gtccattgtg
     1261 tcagtccgct acaatcaaaa gaggtagtcg gggaagctcc gccatagaca tacttccagc
     1321 cttcaaattt cagagcttcg tccatgatgg cttgtactgt atcatcatca aactctgttg
     1381 tgacaagata ctgcgttacc agttgcacat aaaacatatt cccgtaattg tatcgccagc
     1441 ccccattgat aggtatggct atgggattgg ggtaagacac tttttcgtca cccgaatact
     1501 cttttgagaa actttgagcc agttcaaagg tatatttatt cccacgatta gccacatatc
     1561 ctaagaaacc accaccataa ttgtaggact ggataaccga ttctaaatct acaccaagcc
     1621 tttcgctact ggctaataac tcactgaaat acttcacacc ttgcttaatg gattcttctg
     1681 tactcaatga attaggtgga agaccgaggg attccgagga ctgcataaca tcttctgctg
     1741 taccacccga ttccacctgt ataatcgcaa gaagtatatt gacgtattct tcaattccat
     1801 attctttggc aactttttct accataggct tatgagccag cacttctgcg gaaacatcca
     1861 caccgccata gtgaatgttg gaaaaaccgc catcttgttc atctgaaaat aaaatggcga
     1921 caaacagaag cagtgagaag accatcaaga ataatcccga accaccaatc actaaagttt
     1981 tcagcttcat ggtttcttac tgcctttctt aatggtggcg gttttgattg gtggtctact
     2041 ttttgtattt tgtagtggta ctctttgaac agtagactga cgttcttttg tgattgaacg
     2101 ttgtgaagct ctatctgttg gtgcagttga agttactggc ttttgaaccg ttctttcttg
     2161 aactgtatta ctttgacgtt ccgtttttaa acttgaagaa tcggacttaa ctgttggacg
     2221 ctcttgtttg gcttgttgag attccctata ggaagcctgt acagttgttt gccttgaagt
     2281 ctgtccatca tgagattgtt cttgcttagt ggctggtctt tcatgaacgg aagaagctgg
     2341 ctgttttttc tgtttgacct gttccatttc agagcgacgc tccgcaatgg ttttccgtct
     2401 ttgttcctgc tgtttcttgc gtccactggc tttatccgct ttggtttgag aaatacttct
     2461 ggttaaatca tggacattat ccgtcacttt agattttcct tgatatactg catatcttgc
     2521 attggtcggc aaatccttaa cctgttcttt caaattacca gcagtatcta ccattctgtc
     2581 tttggtatca gctactgtac cgatggtttg accgatacgc tttcctagtg ttgatttttc
     2641 ctgcccgttt ggtcgagagt gatctgcttg tgtactcgca gaacttcccg aacccgactg
     2701 tcctttttta cccgtagcac tggcaatggc agacccagcc cctaaagcag tcacggagcg
     2761 tccaagtttc cgctgtagac ggtgcatgtg agcgtgcata agcatacgag gttttctcat
     2821 catacgactt cccacacttt gagaatcgtt actctgtaga gaaaacatac tcattaaatc
     2881 gcccagctta aagtagattc ctgcaaaggt cacaatctgt agaaaagcaa tcaaaaagaa
     2941 cggataacca gccgataagg tatagagcat ggttgaaata ctaaatgctg tcgtaataat
     3001 caatgtgatt ccagctcgtg tcaaaatggt attaaagagc tttgttatgg ctcgttttga
     3061 cataccatca aatgatggaa tcatgcttaa aataaagctc acaggcagaa acatagcata
     3121 gatgataaaa agtacctgcg agaaaatcat gattcctgtt aataggaata caaatatgga
     3181 aatcccaata ttgaagacaa ataggaagaa gactgtacct aaacggttaa tcgtctttgt
     3241 aatcgtcata ttggtattgc ttctatcttc aatttcttcc gcaacaattt tttctctgtc
     3301 ttccccattg ttggaatctg gactggttga gagcaggctt tccacacggt caataccaat
     3361 actttcaatg tctgaactgt tatattgaag cagtagccac ggttgctgaa cctgtatgga
     3421 aaataggctg tcacgtatca agtccacact gtccttgcct tgactatctg aatggggcat
     3481 gacaattttt gtacccagtg acaaactagc gttgctgatg tctgatgaaa agtcattgat
     3541 ttttttaatg tagtcgggag cgtaggcaat aaaggaagcc gataggataa aaaccagcac
     3601 aaaattcatg agggcatgaa ttgcctttgt ggtttctctc tttatcagtc ctgtataggc
     3661 aacataaacc ccaagaacca aaatcaagag taaaaggaat ccaacataga aaccctctgt
     3721 tgatagtcca ctaggcgtaa cccctgcaag ggtctgcata ttcttgccaa tggaatctgc
     3781 tgtagcggaa atgaagtcta aggaataggc ttcctgtact aagtaacccg tcgcattgga
     3841 aacataaaga ctgattgtcc aaataaaatt ggtaatggca tatagtccat acatgacttg
     3901 ttttccaatt ccgtccgacc agttccacgg tagccagccc cagctattat ccacataaaa
     3961 atccagttga tagttttcaa gtgggtatcg gctgtattca tttgccacat tgaccgtatc
     4021 atctaccaag cccgcagctt gaaccaccgt tcccagcatg gctaaaagaa aaatggcaat
     4081 cacaagtgtg aaagccactg tcattgccac tttacctaga cgtttcagcg tccagtttga
     4141 ttttattctg tttaatattg atggtttcac atttacacct cttttcgcac aggtggtctg
     4201 gtatcaaagg catggagcag ttcttcaaat acagggtgga actgtatcac accgacacga
     4261 ccatataaat cactgataag gcattgcccg ttttccaaat cacgcaatcg cttctgattg
     4321 ttttcgtcct ctgggtctac accaaaaaag gcaagggttt ttttaatctc gttaaggtca
     4381 gtggaacgaa aggcaaattt taagccgagg ttatttttca gtttttcatc taagaggtca
     4441 tctgtatttt gggtcacgaa atatacccca gcgttcatag cacgaccagc acgaaccagc
     4501 ttcatagata gtgttttccc ttgtgccacc tgtaagaagc tccatgcttc gtctaagtct
     4561 acaatcttga aaatacttcg gtctgtatgg ataaagtcca acgcaaaggt actaatgaca
     4621 atcagcattg ctaccgatag taactccatt gtggtatatt cctcaaagga agtttccttg
     4681 tcaggaagta ccaagtcggc aacctgtata atgttcagtt gtttttcaag gctgatagac
     4741 tgctctacat aaccatcact aaaaagcaga tgggcaaagt catagtctgt aaaactttcg
     4801 atatggtcgg ctatgctggt acttagtggc gtattctcaa cccgtaattc ttcaatcact
     4861 ttcatcaacc ctcgcacttc actattggtt actgcacgaa tggcttttct aaggattggg
     4921 aagcgttccc catcacgaga ggaaatcccc gtaaggaatg tcaaaatatc aatagccagt
     4981 gattcagaat ctttggtatt tttcataatt acataagggt caagtaagcc tttgtttttc
     5041 tcatcagaag tcagattgac gatattgatt tcatgggaaa tctctggcaa ggtttctttc
     5101 catctgccac gttctgcttt tgggtctaca atcactgctt gtgccccata aagcactgca
     5161 taatagacga taaggttatt cgcaaaggat ttaccaccac ccagcgaacc gacaaaggca
     5221 gacgctaacg cattggttac tgaaccctta accccttgac tggcaagagc aggtttcaga
     5281 tagacattgc gtccagtatc taagctgtag ccaacataaa tcccctcatt ttcacccagc
     5341 atttgagtag caccaaaacc taaaccagcg aggaaatcag aggtcacgta ttgaatatag
     5401 tcattcatat atcttttgct ggcaggtaaa aattcttcat gtaagcccag catatcccca
     5461 aaaggtcgta ccagtttcac gctcaaatca tcatagaaat ctttcacttc attacaacga
     5521 cgtttaagtt cgtcaaggtc atttgctgat acccttacaa cataggacag cttatacata
     5581 gattccttgc tttggtctaa attggtttcc agctcgttca cactttccag agcttccgcc
     5641 acattagagc tggtttcatt atcactttgc caagcgtggt tatccaagtc tttcagttct
     5701 ttctttttat tgcggacagt agatagggct ttacgattcg ctacaatttc cacattcatt
     5761 gacgtatcaa tcggaaaggt aaattgctgt tgctggtagt agaagatttc agaggacggg
     5821 aagtccagtt cgccgacaat actgttgatg gtaaagtaag ctacataggc ggtttcgtct
     5881 tcctgctgga ttttcaaata tcgctgtttt tcttccacca gacagcgagt aggcttaatg
     5941 aggtcatagg ttttaatcag tgtttcatgt tccagctttt tctttgatag aggatactca
     6001 tactcttcat aggcagtgcc tgtctgtccg taaaggtgtt caatcagata gccgaagtca
     6061 tccttatcta atcttcggat tttgaaacga cgggagattt tattttctaa aagcttttcc
     6121 atcttctgaa aacgcaggat ttcatcatta ctcatactaa caaaatcgcc catcagctta
     6181 tggttcacat catagacaaa atcagtaaaa gcattttttg cttcaacggt aagacttttc
     6241 atagaaaact cctgatcgtt gagaagcaac ttaaaaccga taaagaaacg gtagtccact
     6301 tgattttcgc caatcatgga tattaaagcg tctgtctgtt ggtcgatttt gtcataggca
     6361 accgttttga gctttccagt gacttcattt ttggaacgtt cttgtgcaga aagtatgctg
     6421 gattctgtac tgatttgtaa agcatgaatc ttgccatcac gattttgtgc gataagctgt
     6481 ctgaaagaat catgtacttg tattttctgt tccggactta gaaatgagta attgtaggga
     6541 acaagctcat agtaagcgta acattcccca tctttattcc agaccagatt gttttcaata
     6601 tatttaattg gatatgccat aaaattcact cctaactgct gtaatggctt cttgtggctg
     6661 gtttctgcca agcgttactt tttttcctgc ataggtcagc tttcgtcgca gtgcataagc
     6721 aatgacagac ttcaaaaatc cataaggctt tttaccatca aaggtttttg tagacataaa
     6781 ccatgtgaaa gccacaggaa tcccaaagta tttgagaaat gctccctcta tcatagaaag
     6841 agggggcaag ttgccaagta tcataactgc aaagagtgac acgacaaacc atgtcatttg
     6901 cgtaaaggtt atgggaaacg gaagtctaaa gtcattgatg gagtacaaca ctttctcaac
     6961 agaccagata ctggtatagc ttcgtatttt cttcatgtaa tcaatccttt cataaaaatg
     7021 gggatagtct tttgactacc ccgtaaacaa gaaaatctgc cagtaagaat gtaccgacag
     7081 atttaataga cgatttcaaa aatcccgtga ttggttgaga taaacgttcc cgaaaggtct
     7141 aaatcccgac cataggattc ataatcaata tagttttgaa gactggctgg cacttcgcct
     7201 aaagcacccg tttcttcaat gtagtagcgt gccacgtcag acatatcatc acaatcggaa
     7261 tgaatgataa tatcttcttg atgttcgctt agttcttcaa tacttgaaaa atgagtgagc
     7321 agagcagata gctccgattg tagttcttct ggcaactccg ataccatttc ccataggcga
     7381 ttgagttcgc taatagaagt gtattcgtca accgtaaagg gtaactcgta atcatgaatg
     7441 gcgtattcct catattcatc attcaagccg attttctctt tgacttcctc aaagtcaatg
     7501 ggaaaggtaa accacgcacc gaccaattcg ccctcattgt atttgcctaa attcgcaata
     7561 tagacttgca tatcgtccat atattcacgt cctttctttg tagagattca aaaatcccta
     7621 ccgcacttcg tttggtgtac cattcctttg cggaacataa gaaaaccact tatattccac
     7681 aaaagaacgg ttttatttaa gcaccaataa tgcgattgaa tagctctagt aaaatgtctt
     7741 ttactccagc agcgttgaag actaagccaa ccgcaataat cgcaataatt aaaaagccaa
     7801 tcagtttgct aaactcacgc ttgaagccaa gatacaagcc aatcacaacg attgctaaaa
     7861 gcaccagtga ttgagcgttt gatagaaacc agttatatat ggataggtaa gttcctgata
     7921 ttataaatat ctcaaacttt tccccccatt cacacggagc gtgcgacttt caccgcactc
     7981 cgcgttccat cgtgtttata ttgaaaagta tttatgtagt agacctatat caagtttaat
     8041 tcttgattgc cagctaatac tctatattta tttagtctta tgagctggac tttatttagc
     8101 ttcaatagag ctatatattt ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt
     8161 accgcctctt ttaccaaaat aagattttta taatcgtcat ttccaccagc tccagttggt
     8221 acaatatgat gacaatgtat ttcattagct tgttgaaatt cttccagagt tatagcacat
     8281 tttccatgtt gagcagaaaa tagcgaaatt cgattgtctg cgtattctat tgtgcgattt
     8341 tccaaagata atttcattaa ctggtacatc agtaatttat caacacttag attggtgtgg
     8401 ataaattttc ttccctctag agtgtagcaa ttcgctaaat acacctttgc cattggtttt
     8461 ctgtgttgga tatatccaac agggaaaata ggttcgccct cgccagcaac ataccgaagc
     8521 attttagatt taccatatct tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc
     8581 aatctgcccc ttttgtttgt acctaatctg ttagtgaaga tagccattac agctctattc
     8641 agtttcatac aatccagatt tacattcgtt gcaatctgat aataattttg catacctaat
     8701 accattgaat tataaagtct tgtttcttct aattccgttt tcccttttga gggagaagca
     8761 attttctttg cctgtgatac cagcttgtct ttttggtttt tgaattgttt atcacttata
     8821 tgtgatttaa caactaactt ctttcttctc ggtatcattt taatcttgaa accaagaaag
     8881 tcggagtatc tttttctaac atttacaatt cttgtttttt cttgtgatac ttccaatttt
     8941 aatcgttgtt caatccattg agtgattgct attttggctt tttcagcact ttccttgtag
     9001 cggcagaaaa ctctaaaatc atctgcataa cgaaccatat acatttcctt taactttgtt
     9061 ttcttcattt ctttgtaagc gttgctttta catgggctac cgcttttatt gatatgaacc
     9121 acatagtttt ttgtaacagg attttcctgc cattggcttt ctacccaatg gtctaactca
     9181 ttaagaacaa tgtttgctag tagtggggat attattcccc cttgcggagt tcctttctct
     9241 ggatatgtta ttgtaccgtt agtcattttg attggagctt ttaatatcct ttttaaaatg
     9301 aatatcaagt gcttgtcccg tattcccata gcccatatct gttttatcag cttggaatgg
     9361 ttcacgttgt caaagaaacc cttaatatca aattcaatca cataatgtag ctggctgatt
     9421 tgtaggcggt tataggttgc ttttattgca ttttcaactg acctattagg tctaaaacca
     9481 tagctgtttt cactaaattt agcttcgcag attggttcta atacttgttt tatacattgt
     9541 tgtataagcc tgtcccacat acaaggaata cctagaggtc tggttttacc atttggtttt
     9601 ggtatttctt ttcgcctcac tggcttaggt cgatacccat gtggactgcc tgcaacaatg
     9661 tatcttactt ttttgacaac tgtttctgct ggcaatttgc caatatcttt aattattgta
     9721 ccgtctgttc ctgctgtttt actgcctgca tttgctttga tatttcgata tgccagtaat
     9781 atattttctc tcgataggat aatatccata agatgagtga aaatatctcc attcttactt
     9841 ttggcatata aatcatcaaa ggtgttttgc atttcgtaat attcagcgtg tcttaaatct
     9901 tctacacaca atgtttcatt tttattttta ggcataaggc ttcactcctt tacttgaaat
     9961 tgagtgagct tttttatctt actcgtaatc cttattgaaa caacataata cttttctaat
    10021 atatacacga cttgagggca ttcctccatt ctcattacaa gaatttctta ggttcgccct
    10081 ctgcttttca gcattagttc aactgcttat tgttcttcgt cagtttattc tcttgatgag
    10141 aacccaatac ctttcctcgt tccgataatc ttatctatac atatatatcc ttaggtcact
    10201 agctataagc ctgctggctt gataccgcct gtaacgttat atggcatttc ataacctata
    10261 ttcttacttt gccacaccag ctacgatttc tcgtaccttt gcatttctac aaagttcaaa
    10321 tttagacccg tacattcgct gtttcgtcag ttccttatag aacattctaa ccatagatat
    10381 tttatagacc cccgacctgt tggatacgct acccacctct ggatagtttt cgatagaaga
    10441 tatatgttca tcaactatta cggtatcttt cagccgtctt taccgagctt caaacattct
    10501 gagtttcctc atacaatgct tgtcggaggc tcagggcagg cgtttcagag cgttacttca
    10561 tcattccacc tgtcagatta tcagttctcc taataaccaa actttttcgt tcttttatta
    10621 gtgccttagc ttttcactaa ggaacgagtc gcacaaaggt tttgtccaaa attcataaaa
    10681 atgttctcct ctctatattc aatgaatttg tatttgagtt atttttttgt tgttatcacg
    10741 tcctgttctt ttactgactg ttgcttcaaa atctgcttgt gtcggtctgt cagtttcgca
    10801 tggtcgagaa tgtcttttac aacctgcgtc tggttgattt catcaagttt aatcgcaacc
    10861 tttaaggtcg gggcaacttg atgagatagc cagttcagcg tcctttggaa ggagtaaggc
    10921 tctggttttg tggttagttt taatcgttca cgattgttcc caataaacca agcccattct
    10981 tcattcagtt tccaatcaga acgaggtttg gaatcgtctt tatctacaaa acggatatac
    11041 cgattgataa ttttaaaggc ggtatgctct ggattgtcat agacgagtaa atcacggact
    11101 gcataatagg cacgctcatt tttcaatcga atctcaaaac ggttttttac ttctgcgtct
    11161 tcaatgggaa tatcattttt cttgtactgc tcgtagtcct tttcatagat acagaaataa
    11221 acttcacttt gtaatgaacc gatatagagg gtgtttccca tacattcctt ttcctctttg
    11281 cgtaccagtt cgccactgcg atagctttta aaactgcgga agacggagat acattcttcc
    11341 tgttggcact tttcagtgag tacagggata tttaaaatcc ctgtcttatc gttaatggca
    11401 aggtcaaggc gtttcatcac accgccagcc accaaaacgt ccataaagaa ctcataccag
    11461 cttctttgtt gtgccagaag atagctttca aattgtctgc acccacgacc tttcaattcc
    11521 accagaactc ctttgtccag ttcatgggag caaaggacga atatgtcgcc taaagcataa
    11581 tgctctgaat aagaatagaa accatagtcc tcatgaagaa aataggacag tttcagttgt
    11641 aagatgtttt cgaccacctg ctgtacgtct gttgtcggaa agcgaattct tacataatca
    11701 aacagcattt caaggggagc gtcgggattg aagcgttcca gagcttccca aagggactgc
    11761 tgtaaatcct ctgatggctt gacttttcct gtttcaatat cgcttagata ctgccttgta
    11821 ataccagtcg caacagctaa acggttttga gatagtccat aagccaagcg tttttctttt
    11881 aaatgctgta accaagtttg ttcattcagt aaaaatccct ccaatcaaaa aggcgtatgt
    11941 caacttttaa agcccatttg acatacgctg aaattttgta aatcccttgt aaccaaagga
    12001 ttttctaatg tttttttgac tgtttcctgt cgatttgtac ccccctgtta gataccgagg
    12061 ggttaagtgc tggcgtggct attgccacac cagccagcaa gatcagtcca cacctgcgac
    12121 ttccgcttcg cacgtcgcct gcgtggactg tctgctgttg gataactttt taatttcctc
    12181 caagaaatca tatccttttg gtacaagggg agtataaaac tctgatatga cacttgttcc
    12241 tacatcaaca tagccacgac ctttgattcg ctttaagaag aaatcctttt gtacgtcact
    12301 gccaaacatc atgccatagc ccatttcaga catacgacct aaagccactc tgaaattaaa
    12361 ctgatcacgg attccgtcgc ctaaatattt tgcgtctgga cgttgacaag ccagtattag
    12421 aaagaagcca gcttgacgac ctaacatgac aatctgtttc agcttattca taactgcggt
    12481 gttttctttt gttcccagca tttccatgaa agcgacgtat tcatcaaaga ttaagaagtg
    12541 tgccgggaga cctaagtaag cataattttt gccagtctta tagttcttca tctgcttcat
    12601 ttcctcacta cgtttcatca tttcttcata gaatgtttca atgcaagaaa gcaagtcttc
    12661 ttttctatag tagacatttg ccatcacaga acctaagtcc gcaaggtcag catttttcgg
    12721 gtcaagaata tacagttttg aatctgtatg aagcaaggct tcaatcagtg tcagtataaa
    12781 gtaagtttta ccgccacctg taccaccagc aatcaacata tgagggagct tatcatattc
    12841 ccaccatacg tttttcatta agcgaagttt accatcttta gcttctactt catcaataga
    12901 aatacgactg gctatggtgt catagagcaa agtatattcc acataggaat cctttaactc
    12961 tttatccgtc agctcacagt acaagccact ctctaatttc ttttccaagt gtaagagttg
    13021 gtcttgatat tttcccagcg tgatttccac ccgtatctgt atcaagccat ttttaagtcg
    13081 ataatacatt ttagggaagt aggttatctt ttcctttgta cgaccagcac tatctttaaa
    13141 gaaaccctct gttttgacct gttcagattc ataccacttg ttttcaagta tcatctttgc
    13201 cagtttttga cggtggtaaa gttgtttaac cgtatcatag cgaacccgtt tgaatacaaa
    13261 cgctaccagc aagcagataa gaattgcgac actgaaactg ataattaaat agggaatgtc
    13321 aatcttatct gcttgtgata ggttaaaatc ctgccagttg atctgctgga ttgtcttcac
    13381 atgaaacagt ccgacaacca gcaggaaaac aggcaggagt gacgctatcg taaaatgaaa
    13441 gactaaatct ttaccagatg ggcgaatcct tttaccacgc tgtttcatgc gaaaaagtct
    13501 cctttctacc tagcgactat ttgtcttgtg tcggttcttt ctttgcttgt ggttgagctt
    13561 tgaatgaact agaatccttt gtcagcacaa tatcgtctgc cttgatatac cagtcaacat
    13621 ctgctccttg ataggtggca gtagcaacgg tgtccgcaat gggattgata agttccaccc
    13681 gtgcgttata atcaaactct ttcaaaggca cgctggcagg aatacttact tgaatcatgc
    13741 gtccttgtcc tttggatttt aagtcatagg tacgttcctt gatttcatct gaaaccgacc
    13801 cgtcttcatt ttggattctc acttcacgac gtagagcaga gaatttcaat tctccaaaag
    13861 tcgtgtcttt atctaataca atgccatttg ctaatctcat catttttcct ctctttcttt
    13921 attcttttat catgtcgtca gcatgtaaaa ggtaatttgt aaaaccacga gtgccgattt
    13981 tgtagccctc tgcggtaata cgtggattga ctaacttcac acgttcctca aagccgaaat
    14041 gtttttcgcc agcttcagca ggaagcacca ccacaatatc atctgctctt tgaacatcag
    14101 aatagagatt atagcttctt gataagacag ttagccgtcc gttgattctt cgctgaacga
    14161 ctttatcctc gccagcaaat tctaaattgc cgaatgtttt ttccatgttg ggaatcacaa
    14221 atttaagttc catattttta cctatccttt cttttttatt ggctgaatga atgtttgatg
    14281 gtcttaaaga gtggggaacg accttttgat tcttgatttt ttgttttcat aagttcactt
    14341 cctttcaaaa tcgggtaaaa aaatagacac ctcatttttt gaagtgtcta cctattaaat
    14401 attcaaattt tattggaagt atctttatat cttcactttt caaggataaa tcgtcgtatc
    14461 aaagctcatt cataagtagt aaattagtag taaattgagt ggttttgacc ttgataaagt
    14521 gtgataagtc cagtttttat gcggataact agatttttat gctatttttc tcttaaaaaa
    14581 tatcattatt tgtggtatgg agcccccgcg ttgatccgga aggcacggta gatttgctcg
    14641 acgaggatca ggcgcatcaa ttgatgtgga tacgtcattt tgccaaaaga gatctgagca
    14701 ttgcttcttt tcatgacggc ttcgctgagt cctaaagaac cgccgataac aaaaacaagg
    14761 tggctttttc cttggatacc taattggtcg atttgtttag caaaagattc gcttgtggga
    14821 tttttccctt caattgctaa gccatagacg tattcttgtt ctttgatttt cgctaagatc
    14881 cgttctcctt ctttttcttt tacttgtctc atttgcgctt cgcttaaatt ctccggggct
    14941 ttttcgtcag gaacttcgat caattcgatt ttcgcatagg cattcagccg tttgacatac
    15001 tcattgatac cttggatcaa gtatttttct tttaattttc ctactgaaat aattttgata
    15061 ttcatgtttt ctctcctttg atcttcacgc tcactttgtt tattataatc aattttttcg
    15121 tctatatgtt tattttgact atttttgtta aaaagatttc cccaacagtt atgcacaaca
    15181 tccacaggtt tatccacaga aaacagcgtt gttttacctt atttcatcac attcattagg
    15241 caaacatacg ttttataaga atttatccac tttatacaca ggctgtggat atgttttatc
    15301 ccttattttt ccaatcctat ccgcgtagtt ttacacaata tctgtggaaa aagaaaatca
    15361 aatattttca tctgaaaaca atgatttttc ctaaagtttt tcataaaaag ttcaaagtct
    15421 caaagtagac taaagtcaac aaataagact atactaacag tagataaatg aatgtaattg
    15481 gaggaggaac acatggatag aaaaaatgtg acaccaaaga tgaaaaaaaa taaaaataat
    15541 agcctctggc gtaagttggg tctggggctt gttggaggga tcgttggagg actactgaca
    15601 gctggcattt tttatgcagc gatgggcact ggcaacaata gttctactgc tacaagcggc
    15661 aatcaaaatt ctgccggtga aacagttgtg gaaaatgtca aagtcaatgt agattctgat
    15721 atcaccaaag cggtagataa agttcaagac gctgtcgttt ctgttatcaa cttacaaagt
    15781 caaaaccaat caagcggttt tggcggatta ttcgggcagc aagaagaaag tagcagctca
    15841 gatgatagta atttggaagc ttatagtgaa ggaagcggcg taatctacaa aaaagacggg
    15901 aatactgctt atgtagtcac aaacaatcac gtagtagatg gacagcaagg tttggaagtc
    15961 atgatgaaag acggtacgaa ggtaaaagct gaattagttg gaacagatgc ctattcagat
    16021 ttagcggtgt tgaagatcaa ttcagacaaa gtcgaaacgg ttgcctcttt tggggactca
    16081 agtgctttga aggttggtga acctgcgatt gcgattggtt ccccattagg atctgaatat
    16141 gcgaattctg tcacttcagg aatcatttct tccttgaacc gacaagtaac aagtacgaat
    16201 gaatcgaacg aaacagtcaa tatcaatgcg atccaaacag atgcagcgat caacccggga
    16261 aattctggtg gtcctttagt gaatatcgaa ggacaagtta tcggtatcaa ctcaagtaag
    16321 atcgcaagta cttctgaatc gacttctaat gtaagcgtag aaggaatggg atttgccatc
    16381 ccaagtaacg atgtagtgaa tatcatcaac caactggaaa aagacggaaa agtgacacgt
    16441 ccagctctag gtatcacaat ggtcgactta tctgccgttt ctactcagca acaagaacaa
    16501 atcttgaaga ttccttcatc tgtaactaac ggtgtcatcg tgacaaatgt ccaaacagct
    16561 acaccggcag aaaaagctgg gttgaaacaa tacgacgtca ttactaagat cgatgacaaa
    16621 gaagtttctt ctggtgtaga acttcaatct gctttgtata agaaaaaagt gggagattct
    16681 gtcaaaatca cttactatga aggctcgaaa gaaaaaacaa ctactgttga attgacgatc
    16741 gatcaatcgg cattaaaaca aaatcaaaat agtgaaaatt aataaaaaag aggttgtgac
    16801 aaagacttgt cacaacctct ttattttgga aatacttctc cgactacggc atcctcaggc
    16861 aatactaaga ttcctttttt ttctggagca tttggcaaat gcaattcacg agcagaacaa
    16921 atcattccat agctggctac ccctcgcaaa gttcctggcc agatcatcat tccatctggc
    16981 atcatggctc ctggttttgc aacgacaact ttttgtccag cttcgatatt tggtgcaccg
    17041 caaacaattt gcaatactgt cccattgtcc acttctgtct cagtaatcga taaatggtca
    17101 gagtcaggat gcgggacgca tgtttttaca tacccgacaa cgatcttcgg ttcatgatca
    17161 gcttcaagct gttcttcgaa tcctgcttct ttaatcaatc gattcaattt cgctaattct
    17221 tcatctgtta agcttacttg gccgttccct tcaattgtta gatgatcgga gatatggaaa
    17281 aagttccaag caacaacttg accttgttca tctttgatcc gagcaacatt ccatttacgt
    17341 tcgacagtgt tttctgttcc tttatcgtcg gctatgataa ccatcaaagt gtctccgacg
    17401 tgtgctttgt tataagcaaa aatcatgttc tttcctccat tatcctttgc tgagatttat
    17461 cttaacaaaa aattgcattc actgcatgag atttttttca tgctttattt ccgaagaagc
    17521 ttcttctgga acaccattta ttcgtaacaa gggactgtga caagactttt gtcacagtcc
    17581 ctttactgtt tgattaaaaa ggttatacaa gttccacttg cttaattact cggctgttct
    17641 tcttcgtcag aaacgatcaa gacatcacaa ggtgaatgac gtatgacgta gctggctaca
    17701 cttcctgtca taaagcgttc gactgcattt agtccagact gcccaaccat gattagatcg
    17761 attgcatatt tttttggcaa tttgactgcc atggattcac gaacagagcc gtatgcaata
    17821 attccttcta tatttgtgta atcaacagat tttccatatt ctttacattc ttcgatcatt
    17881 tgcttggctg tttcagtttg ctgatcgact agattttgat caataggagc atagcccata
    17941 aaattataaa attgctgatc gattacgttt gctacatata ctttcccatt attacggcga
    18001 gccacttcta acgctttttt gaaagcagca agtgcttgtt cactaccgtc aatacctact
    18061 aaaatgttct tatattcttg attcatcttg acccctccta acagatgtca gccttttatt
    18121 acactttcta ggaacgattc gatttcctgt tttgttttac gatctttatt cactagacga
    18181 cctaattctt ttccgtcttg gatcacgaca aaacttggga taccaaaaat gttccattca
    18241 gcggctacat ctatgtactt gtcacggtct acttcaataa attgccaatc agaaaagtca
    18301 ttttcgattt caggcatttg cggtttgata aaacggcagt ctccgcacca gccggcagta
    18361 aagaaaaata cactttttcc agtttctacg tagcctgcta attcttctaa actatttggg
    18421 ataatcattc tttttcctct actttctgtt tatgtatttt tcaattaaat tatacacctt
    18481 tctttcgaac taacaaacat tgatcctctt tttataaaaa aaatctgaca aaaggttgct
    18541 tttgccagat tttatagttt cactatcgcg ttatttcccg tatacgatcg tgttcactgt
    18601 tgatttatct aaacctttca atgtttgaat caacatttct cgtgcagctt caaaatcgcg
    18661 aatactgaac atcgtttgat gcgtatggat ataacgtccc actacgccaa tcactgtact
    18721 tgggattcct tcattttgtg tatgagcagc acctgcatct gtaccgcctt ttgaaacaaa
    18781 gtattgataa gggatgttgt ttgtttcagc gatatctagc aagtactcac gcaaacgagg
    18841 aagcatgatc aaacctggat cttggatacg catcaatgta ccttctccta aatgtccgaa
    18901 tgtgccattt ttcgtcactg tgtcatctgc tgcagagcag tctactgcaa agaacagatc
    18961 tggcttgaat ttattgacag aagctttaga accacgcaag cctacttctt cttggacatt
    19021 cgcgcctgca atcaatgtat gacctaatgt ttcattttgt aacgcttcta aagcttctaa
    19081 taccattgta cagccatagc ggttgtccca cgatttgctg atgatattct tcccgttggc
    19141 tgttttgatc gtttcagcgt aaggaacgat tgtgtctcct ggaagaacac cgtaactcat
    19201 tgcctcttct cttgattcaa acccagcatc gaacaaaata tctgttactt ctactgattt
    19261 ttgtccgctt gttcctcgta ataaatgtgg gggaacggaa gaagaaatac atggataatt
    19321 cttaccagta gaggttttaa gtgtaaaacg ttgagcagaa acaacgtaag ggttccatcc
    19381 gcctagaggt acgactttaa aaagcccgtt ttcttggatt tgtgtcagca tgaatccgac
    19441 ttcatccata tgtgctgcga ccattacacg aggtgcatcc gcttcttcat ggtggcgcag
    19501 cccaaaaatg ccgcccaaac catctaactg taactcatct accagcggtt ccatatgact
    19561 ttccatatag gcgcgaacgt cttgttcaaa accgcttgtt ccttgtaatt ctgttaactc
    19621 tttgattcgt tgaaatgttt tttcttccat atgctattcg tctaaatttc agactaaact
    19681 cctctcagac ttatattgat ctgcggataa aatatgattt tttctgcaga ccgatcgtat
    19741 ttattatagc ttattttttc tggtagcggt tatttatttc aaagaaaata tgagagtgtg
    19801 gtaaaataaa cacaaagagt agcaagtgag aagaaggagg tatatccaca tgtatgaaga
    19861 aagcgaatta agctatttta aaggcgggtt agccgttggg atcggtctag gattattgag
    19921 tggggtagct tcaacgattt ggtatcatcg caaaaaagtg ttatcagcag accaagtgct
    19981 tgaaaatatc aaaacatctt ttttaaaaga aggcgcaatc gaaggtgctt ggatctcttt
    20041 tgaaaaagaa cctattcgga aattcgccgt tcggtcgagt gcttatcggg gtgggctttc
    20101 ccggatagaa gatggcgaaa ctgtttatta tgaattttta gcagatgctc atacaggaac
    20161 cgtattagac atttcaagaa aaaagggcgc aatagattaa tacgtcaaaa aaatgaaata
    20221 acgaatacgc acccactatc taaaagagag gtatcctatc cttcttttat agtgggtgat
    20281 ttttataaaa atagggctat ttcctcttgg aatagctagt attt
//
LOCUS       DAZKCN010000048        19968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000048 DAZKCN010000000
VERSION     DAZKCN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.049"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..270)
                     /locus_tag="ACG8K3_001594"
     CDS             complement(73..270)
                     /locus_tag="ACG8K3_001594"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP9145471.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(416..1294)
                     /locus_tag="ACG8K3_001595"
     CDS             complement(416..1294)
                     /locus_tag="ACG8K3_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9145472.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1333..2451)
                     /locus_tag="ACG8K3_001596"
     CDS             complement(1333..2451)
                     /locus_tag="ACG8K3_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP9145473.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2448..4547)
                     /locus_tag="ACG8K3_001597"
     CDS             complement(2448..4547)
                     /locus_tag="ACG8K3_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP9145474.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4598..5419)
                     /locus_tag="ACG8K3_001598"
     CDS             complement(4598..5419)
                     /locus_tag="ACG8K3_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9145475.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5416..6225)
                     /locus_tag="ACG8K3_001599"
     CDS             complement(5416..6225)
                     /locus_tag="ACG8K3_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9145476.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6246..6740)
                     /locus_tag="ACG8K3_001600"
     CDS             complement(6246..6740)
                     /locus_tag="ACG8K3_001600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9145477.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6752..7252)
                     /locus_tag="ACG8K3_001601"
     CDS             complement(6752..7252)
                     /locus_tag="ACG8K3_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9145478.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7317..10013)
                     /locus_tag="ACG8K3_001602"
     CDS             complement(7317..10013)
                     /locus_tag="ACG8K3_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9145479.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10441..11514)
                     /locus_tag="ACG8K3_001603"
     CDS             complement(10441..11514)
                     /locus_tag="ACG8K3_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9145480.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11511..12338)
                     /locus_tag="ACG8K3_001604"
     CDS             complement(11511..12338)
                     /locus_tag="ACG8K3_001604"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145481.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12339..13145)
                     /locus_tag="ACG8K3_001605"
     CDS             complement(12339..13145)
                     /locus_tag="ACG8K3_001605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145482.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13157..14242)
                     /locus_tag="ACG8K3_001606"
     CDS             complement(13157..14242)
                     /locus_tag="ACG8K3_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145483.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14259..14801)
                     /locus_tag="ACG8K3_001607"
     CDS             complement(14259..14801)
                     /locus_tag="ACG8K3_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9145484.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15059..16048
                     /locus_tag="ACG8K3_001608"
     CDS             15059..16048
                     /locus_tag="ACG8K3_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9145485.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16045..16782
                     /locus_tag="ACG8K3_001609"
     CDS             16045..16782
                     /locus_tag="ACG8K3_001609"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP9145486.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16779..17330
                     /gene="coaC"
                     /locus_tag="ACG8K3_001610"
     CDS             16779..17330
                     /gene="coaC"
                     /locus_tag="ACG8K3_001610"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP9145487.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17346..17978
                     /locus_tag="ACG8K3_001611"
     CDS             17346..17978
                     /locus_tag="ACG8K3_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9145488.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18063..18710
                     /locus_tag="ACG8K3_001612"
     CDS             18063..18710
                     /locus_tag="ACG8K3_001612"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP9145489.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18715..19698
                     /locus_tag="ACG8K3_001613"
     CDS             18715..19698
                     /locus_tag="ACG8K3_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP9145490.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6122 a         4281 c         3478 g         6087 t
ORIGIN      
        1 cttgctatct ttttataatc acaactctct ataatagaat tacgatttat tttatacggc
       61 tgaaaaaatc tatcaccaaa aaaattcaaa cattaagcca aggattatga agtcaacatt
      121 tcgaaaaatg aacatacatt ggtaagcaac tataactatt accacagaca taaagtaaca
      181 caatatatta ctatttaagt gtacgccacc agtgaatatc ctcaaggtat acagcgcaca
      241 acaaaaagaa aaaaggaaac gcagaagcat ttcctttcag tgatttaatg aaaacaacac
      301 gatttgactt agagcctttt tatttgaatt cattttcgct ttatccaact tcatttgatc
      361 atttgattac tcggttaaat acttttgaca tgagtatcaa tgaaacattc attaattatg
      421 gttgcttatt tcttaccgct tttctggcca atgctaatcc gccgattatt ccggcattat
      481 cacctaattc gggcgtaata atgtagctat caattggtgg tatttcaaca tatccattta
      541 taagctcaac aaatttatcc tgaacttttt tcttcatgtg ttcttgtttc attacaccac
      601 cgccaaaaat aattctatct ggcgaaaaca tcagtgttgt attgtatgca cattgggcaa
      661 ggtaaaaggc ttcaatctgc caaaacgaat cgtcagccaa taaattctgg ccttttacac
      721 ccaaccgttt ttcaatcgct ggtcctgctg ccatcccctc taaacaatct tggtggaagg
      781 gacagtttcc tgaaaaattg tcctttggat gtcgcttgat caacatatgc cccatttccg
      841 gatgactgaa cccttccacg aaagtcccat tgaacaaggc accacctccg atacctgttc
      901 ccacagtgaa gtatgcaaca cctgctaacc ctttgccaga accagcaaca tattcaccat
      961 agcaagccgc attgacatct gttgtccatt caatcggaat attgaaatat tgctttagcg
     1021 ttcctacaaa attgaaattt tgccaagcaa gttttggtgt tgatgtgata tgaccgaaac
     1081 tcgttgattt gcattgaata tcaattggac caaagcagcc gaccccgatg cctgccaatt
     1141 gttccttaaa aggtttaaaa aaatcaatga cctgcttcat tgtttcaact ggagttgttg
     1201 tgggaaaact gactctttcc aaaatcgtta attcctcagt accgactgca cagacaaatt
     1261 tagtccctcc agcttcaatt gccccatata tcatcatagg ttcctccctt cgaatatgtg
     1321 aaaaacactt cattaacgcg gcaagcgttc tcgatcagtg aaatgacgtt tagtatttcc
     1381 tgaagcttct gttgaatacc agtaagcgac actagcaaca tcgtcttgcc gctcaaataa
     1441 gcgaacatca tcgttgccga tttgttgtaa agtgaccgat aaatcttcgg taaaacagat
     1501 ggggtcttga agatgccaac gataaagacc atgtcgtggc attgcgtcgt caccaaaggc
     1561 atgaacggca ttcaattttc ccgtttcaaa acgatctctt gtggcatctc tatttgattg
     1621 gtaaggatac cctaaaaagg ctgttgaata gtttttcgca tgtggacgtc cgaactcatc
     1681 gtactcatga aaggcccacg ccccgccaaa ataatcttct gaacctgttg aggttacggt
     1741 cggatactca ctatccccat caatgtagaa tttaaactcg ccttcgcccc accaataacg
     1801 ttctaggcag gtaagcgcaa ggtaagtacc cacgtaatag ccttgaccct ttaatcgatc
     1861 aattaactta taatcctcct gtaatttggt aatgcgttcc cgatgccaat ttgcgtgaaa
     1921 atataggcta ttttcttcca attcgtccac caaagcgtaa tttatcgtat aaaaaaatga
     1981 ggacatatcc ttcggatgta aattctcgat cgtaatttta gcggatgttt ggaaaggcat
     2041 ttcgaaatag gaattaaagc cccctgtagg attaaccact atcggcaacg agttcacctc
     2101 gcaacgctct ccaaacccat tacaaaagaa atcccctaaa ggactttcaa cagcagggct
     2161 atccgaccca tcccaataaa ttcttaagat taaatcacgt aaaacaaaac tgccaaattc
     2221 tgttcgatca cgaatcgtca tccacatatg acgaattatt cccgttccag taatatccgc
     2281 aatcacagtt tcttcaccag aagtcagcgg aattgcgcca cgacctttgc ggcttggtcc
     2341 tagctcgctt ggcaacatcg cagctttacc tttctcgcct gttggatttt cgggagtgat
     2401 tgctcgactc tggatttttt taaattgggt aatatttttt agtatactca tctttttctc
     2461 ctttgaatgt gaatcgtttt tcccttttct acgtgaagaa tttttttcgt atcacttatt
     2521 ttcaatggct tttctgacaa atttgaaata ctcaactcgg attgcgtaca ttcaatcgaa
     2581 atgaaggctt gaccgaactt caaattcttc caagaaaacg ttaaattatc aacaggatca
     2641 atttgcaata ctcctttttt gaatttcaat ccgaaatatt gactgatcat taggctgaca
     2701 aaaagacccg atgcccagcc gcatttgccg cagccaaagt ctcgcacgac ttctccttgt
     2761 tgagcctcta agcgatacgg ccaccaccac cacgaaccgt ctaaatctgc caaacaaatt
     2821 agttcttcta atcgggttac gaaagcttga gggttgtgca gctcgctcat taaaacagtg
     2881 ataaagccag gaaaagtggc gccggattct aatccccaag tgatgccttt gatttcctcg
     2941 ctataggttg gattgaaaga actagcagaa aaggccatgg tgttctgata taaatgatct
     3001 ttattatcta aaaattgata aaaaggcatc aacgtagtgt ctgattcttc tccatcatgc
     3061 atcaataaat taatttttcc atccacaatc acatcactta aaaagattaa cccttgctca
     3121 agaatcggtt tttgatagtg cttcactgaa taggttgttt tctcctcatc accgattccc
     3181 tccaaaaatt gctcaccaaa aggaccatcg atagtcattt gtttacaaat tgattccttt
     3241 agtttacagg caatgttttt gtatcgcttg ctaagactca gttgttctag ggcttcaaaa
     3301 atttctgcaa gtccaataca ggccttccaa agacaaagat tgctgccggt atgatacttt
     3361 ccaagtgcaa aggcgtctga taaccaaaca gatggaaaaa gcattggttc ccctgaccga
     3421 cgttgagcta atatgtcctc aataattctt gaagcattca gtagaacctg cggacaccgc
     3481 tcaaagaatt ttgtatcccc agtatgttca aaataaagac tagccagtaa cgcactggat
     3541 aacgaattac tcagagaatg attgatacct cctgtaaatt ttgaaccagg aaacttaacg
     3601 ccatattgat caaaccatag aaccgttttt tgacataact ccggttcaaa catggtgaat
     3661 ggcaatgatg cgtaatacat atccttattc caaattcgat tagtagcagg atagctgccc
     3721 caattgctgc cgacgacctg tccctgtcga ttcataccga aactagaaaa gctttgataa
     3781 agtgcacgac gatagagatg gacaactcta tcgtttggca ttgacagctg cccaaatagc
     3841 tgagcaaaat agttcaatgt ttccttcagc catttttcga tatcagattc ctgaaaagtc
     3901 attacttctt caaaagcatt tgggtccaca aaagcaatgg cgaagcttgc cacttcctgt
     3961 tgtgcgatcg tgtccttatt tcctttttga gaaatcaaca cattttgctg atccgaatac
     4021 ttctcttgat acgaaggcac atctttcact gaaacttgaa ggctttcgtg tgtttcattc
     4081 acaatgtaaa cctgttgaac aagcatactt aaacgcttat tagccagtat gggagcaaag
     4141 gatactacaa tgaccttaaa cgtcgaataa tgatgaatat atctaggcaa acagtcttga
     4201 atcacatcga cttcaatacg atgatccgat tctgacaaat ggtagacaat cccgtttact
     4261 tcaaccttta aggataacga attggtttga gagagctgtt tattgaccca aacacctggt
     4321 ttttcctcag ttagtaaatt ttctcgataa aaggtcaacg tttgaatcgt cccgcaaaca
     4381 tcaaggtttg ctaatgtctt ttgattactt acatcgaagg tcaatccctt tacatcagga
     4441 atattccgat agtagctgta ttcgtttgaa gtagtcacat ttccgtcgtt taactcagca
     4501 ttaaaaaatg ggtgtttggg tgaataaatt acttcattaa tagtcatctt ttccctcctt
     4561 gctaaaaaaa ctgcagccta gtagctgcag cttcctacta tgcgattcca agtaatgaca
     4621 ataaaattcc caagacgacc attccaaaaa taattttgat gacgccgact tttttatcta
     4681 gtaatttgaa acagctcaat gttaccaata acggtacaat tcctttaaaa atactatcga
     4741 ggatttcttg gacattcatt agttcttcgc ccttcattga aaagctcaaa acggtattga
     4801 atttgaccat actgatcgtc attgcaccta ccatgatcag acccaagata ctagcagctt
     4861 ttgtcagaat ttcaattaac ccgttcgcat acagcttttc aatatattta gagcctaatg
     4921 aatagcctaa aacgccgcca tagtatcgca tcaagtaatt aggtatattg aacataacta
     4981 aaaagataat tggtgccaaa atatttccgg cattggctaa gttgatcgct agtcccgcgg
     5041 taacgacacg ccaaactccc cagaacaatg aatcgccaat tccagccaat ggtcccatca
     5101 aactcgtttt gatcgcgttg atactttcca cgtcaaaacc agctttttca gaattttcct
     5161 tctccatcga tgccgcaatc cccatgacaa aggacgcgca aggaatggtc gtcgaaaaat
     5221 actgtgtatg acgttccagc gccgcagctc gatcttcttc acttttgtag aaatgattaa
     5281 tgaagggcat catagaatag gcgaaaccac cgccgcccat cgtcaccgga ttcaccgaag
     5341 catacatagt catcgagcgc caaaacatct tccgaaccaa ttttttctca tctttagaaa
     5401 ttccatcagc taatttcatt gaaagaaatc ctcctcttct tggtcaagcg ttgcatttac
     5461 agcagcatcg ctttgaactg catgaacaga tatatttttt agttgtagat ctctttgggc
     5521 tataacaatt gcaataatcg ttccaactgc ggcaattgca atcaatggta actcgagata
     5581 cgccataaca acggccccaa agaaaaacca cacagcaata tttttactcc aaagcatccg
     5641 taacagcatc gccatcccaa ctgccggcag cagatttcca gcgactgtta atccgttcat
     5701 gatgacctct ggaatattat ccaagacgct tttgacaaca tctgctccaa ataaaacggc
     5761 aaagaatgga ataagagcat aaagcgcata gtggacaaac cacataaaga agtgcagatt
     5821 gatgattcct ttttgatcac cagattttgc aaatttgaaa aatttagcag ccatcggtcc
     5881 acatacaaaa gaaaaaatca tattcattaa ctgtaatcct aatactgcta gtggaaagac
     5941 aatgggaata atcgcatcca cgtttcctcc cattttgatt gcgaacgctg tagccagccc
     6001 agcagccaaa gaaggctccg ccgtactaga gattccgata ttgacgacac ccataaagat
     6061 ggcttctagt gacgccccga caatcaaccc cgtctgcaaa tcccccatca acgctcctgc
     6121 taacggagca acaacaatcg gacgactcag catggacatc ccgaacattt catgtccagc
     6181 aactccaata aaaacggcca aagcgactac aaatgcatca tacataattt ttctccttcc
     6241 tttttctaaa aatcactcaa aatagagata gctgaacgtt tttccatcgc cggtgtaggc
     6301 tgaaaaacgg tatctggata aagttcaact aattctttta agtcttgaat ttctgacgtc
     6361 gttaacatca cttcctttga taaggttttc ttatcttccg atggcggtgt cattttccca
     6421 gcattcccca cgttcattaa ttcgatttcc gttgggaaag ctttcgcaac ttttaccgca
     6481 tctttgacat tagggactaa aaccatcaag cgcatgtcct tcgcccgcgg atcttttaac
     6541 aaatgaatcg cgtcatcaac tgttttaata atgactttga cattcgatgg tgcagccatc
     6601 ttcaatgtca tcttttgaat atcgttggta gccgcctcat cgcttgctac tactaacccg
     6661 gttaaattta gactctttgt ccaagtcatc gctacttgtc catgaattaa tcgttcatca
     6721 attcgcactg ctttaatcat ttttcttcct cctaaaaaaa atcttcctct tcatttacta
     6781 catgtacctc atcaagaatg gtcatcttta attcctgttg gcatttattt accagttcct
     6841 gaatatcctc ctttgttaat tccttggttg ccaatactat ttcgagcaga attggcagat
     6901 taaagccaga caaaatagtt acagcaatct ctttctccaa aaaggttttg gtaatcttct
     6961 gattgacact tccgccaaat aaatcggtaa aaacaaaaat ctgatcctgc tcgttcacct
     7021 ggtttacaaa tacattcaac tcctgttcaa aatcacactc atccacataa gcattaatca
     7081 ccgtaatctc ttgaaccttc cccgtcagta attgaatact gctaagaaag cctgaagcta
     7141 agtgaccgtg agtcgctatt agaattttct tcatatggtt aattcctttc tttctgttca
     7201 cttttatata agcaattcct gtgccaactt ttaaaaacat tggtttaatc acgttaattc
     7261 acattaaaaa aagtgtctaa cctaaaaaaa acagattaga cactttttta gacactttat
     7321 ttttgaatat acggtcctaa taattcatag agcagcgaaa gctcataagc attgacttta
     7381 aaattatatt tcatctccat tgattgaaaa atacttctag aaacataata aaattcatct
     7441 tcatctggtt tcaaatcata attcggttct tcagattttt ctgtccgagc caagaataaa
     7501 cgttccatca tcaatgccgt atgcatatag agatttagtt ttacacggcc atttaaagtg
     7561 aaatgatagt aattttcata tttagaaatg actgtttcca cttcactcat cacgatcgtt
     7621 gggttcagaa agttcaaacg gtccgcaatt ccttccaacg aaaagaattt tactagctcc
     7681 tgaatcaagc ggttaaattc tgccggcttc atttcaggac tcaaaatatg ctccaaattg
     7741 gctgctccat caccgtctaa gatgtcataa acattaatat taggaattga aaaggaacgt
     7801 ggaagatccg aagtcgtgat gaccaatttt gtcttgtcaa aataagtttc atcattttgg
     7861 tcgattaatt cacgtagttc tctgtagtcc ttcgtgaaaa cttcgatgga ttcatccgat
     7921 aaatgatgac gaaaaacatc tttcaatttg tccgaaatcc ccaaacccga catacaggaa
     7981 atgataatgt tgctactttg agaaaagcct tcaaagtatt gaacattgat tgaatacttg
     8041 tctgctgcat cctccgcaat ctgtttaaat ggcgcattca tcgtcatctt caagccaata
     8101 tcaagtgcaa tggaagtcgt caaattattc agaagaagta actccccttt taaatcgttc
     8161 ttgatctgtg tgtaaatttg atttaaagag cccatatcta cgatcagcac tagactatct
     8221 gtattcactt gccgtttcac gaaattcctt gtcgattcga caatctctga cacattactc
     8281 tctactggca tatccaacgc ttcaaaaaca taattgccgc ataattgatt cactactgct
     8341 tgaatgctgc tagccgtact atctccgtga gcaagcaaca ggcccttcag ctcgatggat
     8401 tcatctacat aatcagataa agtcacgaca ataagcagtc ttagaaagtt ctcgtccttt
     8461 tctgagagcg gtggtaaatg ctccaagaac tgattacata agtaagtcgt ccgaggtaga
     8521 tttgcagcta atgtacgatt tatcccggtt aagtctgtct tatagcttgg ggaaatgaca
     8581 taggatctgc taataatttc tgcaacaggt ccactcgtcg tcaatccgta cgtttttcct
     8641 aaaacggata cccagacctt tctaaacatc tgaatcatca attttctact cattgataaa
     8701 aaagaatcct tatcaatgtg atgcaccaaa tgctgtactt cattctttat cgaagaaaaa
     8761 ttttcattga aattaccttc atttatcagc tgagtaattt tttccaaaat cgaatcataa
     8821 tcaaacggtt tgattcgatc cgtttttatc ttttgatttt tctttatcaa taaactagca
     8881 atcggcaagc tttctacttc ggattccagc atatttaact gaatcaagat ctcatttttc
     8941 ccaatcgtta atggttcttc ttgtgttcga ctatacacat ccgcacaact gattttaatg
     9001 atattcttca gcttaccaat attcccacta aagggatgag ataacattaa agacaaggct
     9061 tctcttgaaa caataatatc tttttgtaaa acatccgctt cattttgaaa aaataattga
     9121 atcagctcta atcgttctcg aatggggcga tcttcaaaag ttggcaacat aacactaatt
     9181 tgaatacgcc gatcaaaggt gtctaataaa taattttcac ctttttccgt cgtggcgaaa
     9241 ataaaacgaa ccttagcaga atgccaattt acattttcac cgactggacg atagtttcca
     9301 gtatcaataa atagaaagag cttttcttga ttttctttcg ataggcgatg gacctcatcg
     9361 agaaaaagat agccgttgtt ggccgttgcc aataaaccat cggtatcctt ttcagccccc
     9421 gtaaaggccc ccttcttgta tccaaacagt gttgccgaaa gcagttcggg attgtccgca
     9481 taatcggcac agtttaagat agcaaatggt gcatcttttt caataatttg ctcatggata
     9541 gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa cacccgtggc tccatttatt
     9601 aatacactga gtccgttagg cggatatttg acagcggcta tacatttttg aatgacctct
     9661 ctcagacttc cgtcatgacc aactacagaa tgaaaggctt cgtttatttg ctccgtatta
     9721 tctacaaccg catattcatc taaaggcttc cacaaaacag gctttcccgg taatttctcg
     9781 acctttttat cttccactaa tctcgtcaaa taatggctgg cattctgacg agagatacct
     9841 agattcttcg aaattttctc ggcagtgaca gaagacatat tccctgttag tcgtatcagc
     9901 tcatcaaaaa ctcgatcttt agcgctcttt ttcatttttt cctccagttt aatgtgtcta
     9961 aaagcataat aaactatcgg ctgcaatatg tctattctat atttgtattc caatttttac
    10021 tttgaaacac aaatagtcga atatttgcct cagatatctg acaaaataat ttatctacca
    10081 atttagtttt tatctaatat gatttttatg ttttttcata gctacaaata ttgaactgga
    10141 ttcatatatt agttttatta atagattaca gaagggttaa gtatatttgc atagtttcca
    10201 attactttat ccttctgtaa ctttttcaat cttagattta ctagcccgtc tcgggcccta
    10261 ttttacaatg gttttgtcca cttttctctg gtagaaaaac gtggtagaaa aaataaaata
    10321 acacaaaaaa agcggcgttt catccgcttt tggtagaaga ttggaaaact cagatagaaa
    10381 aaggacgatt tttagctctt tagaacgttg ataagtcccc attcttactg agttttgcca
    10441 ttatctccta tacatcttga attctaagaa taggtcatta tagatgccta ggtatttcga
    10501 tcctagatct ttgtagactt caagatggga gatcgtgtca tcagatggat agaattgttc
    10561 gtccgacgaa atgtcttttg gcaataacgc ttttgcttct tcatttggtg tagaataacc
    10621 aatatactct gcattttgtg ccgcattttc cggttctagc ataaagttga tgaaatcata
    10681 ggcaccttct ttatttttca cggtttttgg cataacgata ttatcaaacc aaagattgga
    10741 tccttctggt gggatcacat aatgtaaatg ttcattttcc cacatcatat cggcagcttc
    10801 gccagaaaat gtcactgcca cggaggcttc ttcattgatc atatacattt tgatctcatc
    10861 tgccacgatt gctttgacat ttggtgttaa atgattcaat ttatttgctg cttctgtcag
    10921 ctgtgccata ttcttactat tcaaagaata gcctaaacta ttcaaagaaa gccccataac
    10981 ttctcgtgca ccgtcaatca gcatcaaact gtcttttaat tccggcttcc acaaatcatc
    11041 ccaatgctta atttgatttg catcaaagaa tttatcgtta tatacgatcc ctaaagttcc
    11101 ccaaaaatat ggaatcgaat actgattatc tggatcaaaa tccaagtcta aaaatcgtgg
    11161 atcaatatta tttaatcctt tgatcttcga atgatctaat ggaagtacta gtttttcttt
    11221 gatcattttt tggatcatat attcacttgg tatagcgatg tcgtagtttg tcccaccttg
    11281 ctggattttt gtgaacatcg cttcattaga atcaaatgtt tcataattca ctttgtatcc
    11341 atattctttt tcaaatttcg tgatcaaact aggatcgata taatctcccc agttatagat
    11401 ggtaagtacc tttgctccac tcatgccact tgttttttca agttgacgaa caccaagcag
    11461 cagcaccgca ataatgatga gaatacctaa aaacagagat tgtaattttt tcatcgcagc
    11521 cgtgccaccc cctcgcgtac tcttctgttt ttcttcagcc gttttggacg gttttcttta
    11581 ctaataaagt agtatccaat gaccaatatc atggagaaca agaaaactaa cgcacttagc
    11641 gcattgattt ctaaactgat tccttgtctt gcacgagaat agatctctac tgagagcgtc
    11701 gtaaagccat ttcctgtgac gaaaaatgta accgcaaaat catcaagaga ataggtaaat
    11761 gccataaaat atccagctat gattcctgga gaaagataag gcaaaatgat atttttgact
    11821 acttggaaat tattagcacc aaggtctcta gccgcatcga ccattgaatc gttcatttcc
    11881 tgcaattttg gcaagaccat caagacgacg attggaatgc tgaaagcaat atgggaaagc
    11941 aatacactct gaaaacctaa tccgaatcct agtagtgtaa agaaaatcaa gaaactagca
    12001 ccgataataa catcaggaga gactaataag atgttattca tgctcaacaa cgctgtccgt
    12061 gcacgtcgtt ttttcgtgta atagattccc atagccccaa atgtcccgat gatcgtggcg
    12121 atcaatgcag ataagaaagc cagcatgaac gtattcaata cgataatgat caaacgtgta
    12181 tcttcaaaaa cagcttcata attttcccaa gtaaatcctg taaagctgtt catcgttcct
    12241 ccttcattga aggaataaaa aatcagatag aagatcggtg catataagaa taaaaagacg
    12301 atccctaaat aaagatacga ccatttaaat tttttcattt atgagcgacc tccttttttc
    12361 ttctcgcctg tcaggagcat cacgatgaac atagcaatga ttaaaatcac accgattgta
    12421 gaacccatcc cccagttttg tgtaaccaag aaatgctgtt cgatagccgt tcctaaagtg
    12481 atcacccgat ttccgccaat cagacgagtc agcatgaata aagaaagaga aggaataaat
    12541 actgcttgga caccactttt cacaccattt aatgataacg gaaaaatcac tcgtcggaag
    12601 gtctcaaagc tattcgcgcc taaatctcta ctagcactga tcaaagaagg attcatctct
    12661 tccaatgcat tgaaaattgg cataatcata aatggaatct cgatatacgt tgccacgaac
    12721 agaaaactga agtctgtgaa taatatctgt ttcggcccga tcccgataaa atcaaagaaa
    12781 tgattcacgc tgccgtgcac actgaaaatc ccaataaatg cgtaagcttt aagcagcaga
    12841 ttcacccatg taggcaaaat aatcagcatc agccataatt gcttgtgctt cagttttgtt
    12901 aaaaaataag cagtcgggta actgatgatc agcgtgaaaa acgtgatcaa aaatgcatac
    12961 catacagaat tgatcgtcat agaaagcgat gtccccgacg taaaataaga tagataatta
    13021 ttcagcgtga attgcccatt catatcaaaa aaagattgat aaatgatcat taatacaggc
    13081 gcgatcacga ataaaccgag ccacaaaata taagggacgg aatagatcct gcgcattgtt
    13141 ttcattcttg tcccccctat tcttcgtagc tgtcaagacg tgcatcgaat tcttcttcag
    13201 actcgttgaa acgcatgaca tggatatctt ctggctcaaa ggccaatcct actttgcttc
    13261 cttctttagc ttttttcgtt gaatggacca tccattcatt ttgctgttca tcataacaga
    13321 tgatttcata atggacacca cgaaacagct gagtatcgac cgttacgact agttttcctt
    13381 tatctggtgt cgtgatcgtc aaatcctctg ggcgtaaaac aacttcaact ggttcattta
    13441 gacgcatccc gccatcgaca cattcaaatt gcttaccaac aaactcaact agattatctt
    13501 cgatcattac accgtccacg atattgctct ccccaacaaa atcagcaacg aaatgattga
    13561 ttggctcatc ataaatatct acgggtgtac cactttggac gattttccct ttattcatga
    13621 caaaaatctc atcactcatt gccaacgctt cttcttggtc atgagtaaca aaaataaacg
    13681 tgattcctaa tcgttgttgc agatcccgca gttcatactg catatctgta cgtagtttca
    13741 aatcaagcgc cgataacggt tcatctaata gcaatacttt cggttcattt acaattgctc
    13801 gtgcaatcgc tacacgctgt ctttgaccac cagacatttc actaatctcc cgtttttcgt
    13861 atccaggaag ctggaccata cgcaaagcat cttttacctt cttttcgata tctgatttgg
    13921 acattttctt gatcttcaat ccaaaagcaa cgttatcaaa aacattcata tgaggaaaaa
    13981 gtgcataatc ttggaagact gtatttactt gtcgcttatt agctggcaaa tcattcaacc
    14041 gtttcccttc aaaatagatg tccccttctg tagcttcggt gaatccagct ataatacgta
    14101 ggatcgttgt tttaccacag ccggaaggac caagcagtgt ataaaatttt ccttgttcga
    14161 tctcaaaact tacttttttt aatactgttt catcatcata gcgtttcact acttgatcaa
    14221 atgaaatgat tggtttcccc acgtttttac cccctatatt acaaatatga ttgcgttgca
    14281 acaatcaata cttttgttcg ttttttgaga tgattcctta attgatgttg ttttgtcgct
    14341 tgataataga tcgactgtcc tgcttttgcc acatagaccg tctctcctaa gcttaactct
    14401 aattcccctt ctaatacaaa aataaacgtt tcagataatg aaggttcaaa cgtcttatat
    14461 tctcctgcac cttcaaaagt cagcaagaca ggctccattt ctttttcatt cgaatcagcc
    14521 acgagccatt ccaattcata cccgttttct tcatcgaaat aaaccgtatg atcttctttt
    14581 gaataaacca cttgcagatt cgctgcttct tgatgaaaaa attcttctgg agtcacacct
    14641 aagacttcaa gaatagaaaa gaatgtttcc attgatggtg aacttaaatc tcgttccaac
    14701 tgggaaatat acccttttga caaatctgtg cgttcgccta gttcttcttg tgtcaaattc
    14761 ttttggatgc gcaaatttct taacttctca ccaatttcca taatttcctc cgaatcgaag
    14821 ttttctaata gtaaacttcc tgtttactac cactcgtatt agtatacgaa atttattgaa
    14881 aaaggcaacc ctttgtaaaa ttttttttgc tgaatatttc tttatctttt ggaataaatt
    14941 ctagttgttt gattctttat ttttcaccta atttttgtct aaatttacgg aagaacaaga
    15001 ataaaagagt aggctttatg gtatgataaa agaaaatttt aaaaggagtg tctcacgaat
    15061 gaacgctttt cgatcttttc aggtaataaa ggaacctgtt ttccatacag caatcagtga
    15121 tctgccattt actgagttgg ctgaaaatga agtcttggta aaaattgcct attctgatgt
    15181 caattataaa gatgcattgg caatgagcga gtctggtcag gtgatacgaa catatccgat
    15241 gactccaggg attgatttat ccggaactgt cgtccaaagt aacaatccac gtttttcaaa
    15301 agaagatctt gtacttgcta ctggttttgg gcttggcgtc acccatccag gcggttatag
    15361 ccaatatcaa aaagtaccag gagactggtt agttcctcta ccaaaaaata tgacgctgcg
    15421 tcaagcgatg atccttggta cagcagggtt tactgctatg ttgtgtgtga acgctttaat
    15481 ccgtcaaggt atgacctccg ataaaaaagt tgtcgttact ggtgcttctg gcggtgtagg
    15541 cagtacagcg atagccctgt tacataaact aggattccag tcaatcattg ccttttccag
    15601 aaaagaagag tctgtcacat ggctaaaaag tctaggtgct ttgcaagtcg tacgacctga
    15661 tgaatttctg ccagagacaa caaaagcttt aggaaaacag caaatcgact atgtcatcga
    15721 cacagtaggt ggcgagcagc taagcagtct tcttccattg atctcttata acggtgcagt
    15781 agctttatgt ggcaatgctg ggggaatcaa actaaacgcg actgtcttgc cttttattct
    15841 gcgaaacatc cagttgattg gaattgattc tgtcaatgta ccaatagatc aacgtctttc
    15901 tttatggcaa cagatggcgg atttgcagat tgcagatgaa ctggtcgtcc aagagattac
    15961 tctggatcaa ttgcctgaaa cagcatctaa actacttgct ggaacacacc aaggaagaac
    16021 actagtaaat gtaggtgacc ataaatgaag attctaatca cagctggagg aacgactgaa
    16081 aaaatcgatc aagtacgtgc tattacaaat cattcgacag gacgattagg gcaagcatta
    16141 gctgattatt tagctgccaa tccggatacc acaatcgatt acgtgactac gaaacaagca
    16201 ctgaaaccga aaagacactc caatataacg atttatacga tcgaatctgc actggactta
    16261 tttttacaat tagaagaact aacgaaaaaa gaacactacg atgcgattat ccacagtatg
    16321 gcagtcagtg attttactcc tgcttttagt ttttcagaag aacagctggc aaaaaaccta
    16381 cccacttctt caacgcaaga agaactggac aattggtttg cggaaaatga acagacagat
    16441 accactgttt caaagatctc ttctaacacc gagcatcttg tgctcgtttt gaagaaaaca
    16501 ccaaaaatca tttcttatct acgtgaatgg cagccaaaag caaaaatcat tggtttcaaa
    16561 ctattagtag atgtaccaaa ggaatctctt ctggcagtcg caaaaaatag tctgataaat
    16621 aacaaaactg attttatttt cgcaaatgat ttgacggaaa tccatggcga gacacaccac
    16681 ggctatctct tatctaaaga tggaacagtg gaagaagctc aaagtaaatc tgagattgct
    16741 gcattgatta ctgagaaaat tagactggag gaatcaaaat gaaaaagatc ttactaggcg
    16801 ttactggaag tatttctgct tacaaaagcg ctgatcttac aagtcaattc gtcaaattag
    16861 gtgcccaggt agatgtgatt atgtctaaaa gcagcacaca ttttatcacg ccattaacct
    16921 tgcaatctct gtcgaagcgc gcagtccata cagacgtcat gcaagaatca aatccgcagg
    16981 taatcaatca tatcgaacta gcaaaacaag ctgacttgtt tctcatcgcg cctgctacag
    17041 caaacattat cggcaagcta gcaaatggca ttgcagacga tcttctttca acagtcgcaa
    17101 tggcgcttcc agtgggaaca ccaaaattga ttgcccctgc catgaacacg aatatgtatc
    17161 aaaacacgat cactcagcgt aatctggaaa cattgagatc tgtcggctat caggaaattg
    17221 aaccaagaga atctctttta gcttgtggag attttggaaa aggtgcctta gctgatattc
    17281 agacgatcat cgaacaggtc atgctgactc ttaatgaaag aaatgcttag aaaaaaggag
    17341 tacttatgaa aaatacaaaa aactttacat taactgcgat gtttttagct attttgattt
    17401 tattagctgt tacgccttta ggttttatcc ctatcggtcc aatcaatgca acaaccatgc
    17461 atattcctgt aatcattgct tcgatcgttt taggaccaag actaggtgct tttcttggcg
    17521 gtacatttgg actgatcagc atgatccgta gcacagttat ccaaacacct ttgtcttttg
    17581 tcttttctcc gtttatccca gtcatcggaa cagaacatgg aagctggaaa gccttgctga
    17641 tcgcgtttat tcctcgcatc ttgattgggg tggttcctta tttcgtttat aaaggtctgc
    17701 gcaaactaat gaaacaaaaa gcagattccg tttctttatt cttagctgga ctttccggtt
    17761 cattgaccaa tacgatttta gtgatgaata tgatctactt tttattccag caagattatg
    17821 ccaaagtcat cggaacaaat ttaaatgctg tttattcagc tattctagcg gttattttta
    17881 caagtggtat acctgaagga atcgtcgcag gtcttgcgac tgcagcagta tgcaatgtct
    17941 tgttaagata cttcaagcac ccttctctta ctaactgata gtcatcgtag ccgatctctg
    18001 atttttttct cttttggtgt tactattgaa tggaaagatt tgacgtcgaa aggatgatcc
    18061 aaatgaaaat ttttgttgta ggtgcaaacg gacaaattgg ccgacacctg ataaaagatt
    18121 tagctccaag ttcccatgaa atttttgcag gggtgagaga tgttgcttcc caaacattag
    18181 taaaaaaaga gaatgtttcg tatgtctctt ttgatttaac ttggtccgtg gaaaaaatga
    18241 ccgaagcatt caaaggcatc gatgtattga tttttgctgc cggctctcaa ggaaagaatc
    18301 ttctgcaagt agacttggat ggcgcaatca aaacagtgat cgcagctgaa aatgcgcatg
    18361 tctctcgcta tctgatggtc agtgctgttt atgcagatga accagcaaaa tggcctgagt
    18421 ccatgacaga ttattatatt actaaacatt atgcagatga atggctgaaa cgaactaact
    18481 tagattttgt cattcttcaa ccagtcacat tgactaacga tgaagaagtt acttccattc
    18541 aattaactaa gcctaatgag aaggcatcaa aaacgatcac tcgctctaca gttgctgctg
    18601 tacttgccgc tctagtggaa gaaacggata tttctcgtac gactctagta ctttctgaag
    18661 gtagcaaaga gttgaataca gcattccaag aatgggcaaa ggaggaatag atccatgcgt
    18721 gccatttcaa tcaagcatcc tggttctgta gatgtattag aacaaattga agtgcctgtt
    18781 cctaaaccaa aacaaggcga attactgatc aaagtccata cagcagctgt caatcgaaca
    18841 gatattatga aaagagaatc agtctctttg aaacctccct accctatttt gggagtcgaa
    18901 gtagctggag aggtagttga gaataaaagt gatatctcca cttttactcc tgggacaaaa
    18961 gtatacggat tagtcaatct aggtggttat gccgaatatg ctgtgatgcc tgccgatcgt
    19021 gcaatccttc tccctgatac gctagactat gtttctgctg ctggaatctc agaagttttt
    19081 ttgactgctt atcaaacact ttactggctt ggaaaattac aagaaggcga aactgtcttg
    19141 atccatgcag gagcaagtgg tgtgggaaca gcagcgatcc aattagctag acagctttct
    19201 cacgcgaaaa tcatcgttac tgcaggatca gaagaaaaac tcgccttttg taaagaactt
    19261 ggtgcagatg aattgatcaa ctataaaaaa caagactttc aagaagaggt acgaaaaatc
    19321 actgaaggtc ggggagtcga tgttattttg gatttcatcg gcgcttctta ctgggaaaag
    19381 aatttagctt ctatagccgt tgatggacgt tgggtcttga ttggtatgct aggtgggacc
    19441 atcgtgcctg agatagacct tggtgtattg attgggaaac gtgtccaact gcttggtaca
    19501 ttattgacac ctagaagtga tcagtataaa gcagagctcg cacaaacttt tatgcaaaaa
    19561 gctgggcctt ttctagaaaa tggcaagatt cggccaatca ttgaccgtac attccaattc
    19621 agcgcagtca aacaagcgca tgaatatatg gaagcaaaca aaaacatcgg aaaaatcatt
    19681 ttgaagataa ctgaataaaa tcatgctgat caaaaaagag gctgggacag aagcgtttaa
    19741 ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcagct
    19801 tatttccgaa ggaatttctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa
    19861 agtgttcttt acttttgtcc cacgctcttt ttccgaataa acggtgttca aaagcttgca
    19921 tctgcggtaa taagtccaac caagttaaaa acataagaag ctgttttt
//
LOCUS       DAZKCN010000049        19264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000049 DAZKCN010000000
VERSION     DAZKCN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.050"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..634)
                     /locus_tag="ACG8K3_001614"
     CDS             complement(38..634)
                     /locus_tag="ACG8K3_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP9145491.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="ACG8K3_001615"
     CDS             984..1328
                     /locus_tag="ACG8K3_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9145492.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="ACG8K3_001616"
     CDS             1329..1748
                     /locus_tag="ACG8K3_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9145493.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="ACG8K3_001617"
     CDS             complement(1840..2190)
                     /locus_tag="ACG8K3_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145494.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="ACG8K3_001618"
     CDS             complement(2156..3487)
                     /locus_tag="ACG8K3_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFP9145495.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="ACG8K3_001619"
     CDS             3742..4308
                     /locus_tag="ACG8K3_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9145496.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="ACG8K3_001620"
     tRNA            4453..4540
                     /locus_tag="ACG8K3_001620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /gene="bsh"
                     /locus_tag="ACG8K3_001621"
     CDS             4876..5850
                     /gene="bsh"
                     /locus_tag="ACG8K3_001621"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9145497.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6175..8070
                     /gene="asnB"
                     /locus_tag="ACG8K3_001622"
     CDS             6175..8070
                     /gene="asnB"
                     /locus_tag="ACG8K3_001622"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP9145498.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8265..8711)
                     /locus_tag="ACG8K3_001623"
     CDS             complement(8265..8711)
                     /locus_tag="ACG8K3_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP9145499.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8939..9088
                     /gene="rpmG"
                     /locus_tag="ACG8K3_001624"
     CDS             8939..9088
                     /gene="rpmG"
                     /locus_tag="ACG8K3_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9145500.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9176..9694
                     /locus_tag="ACG8K3_001625"
     CDS             9176..9694
                     /locus_tag="ACG8K3_001625"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP9145501.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9771..10727)
                     /locus_tag="ACG8K3_001626"
     CDS             complement(9771..10727)
                     /locus_tag="ACG8K3_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9145502.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10893..11699
                     /locus_tag="ACG8K3_001627"
     CDS             10893..11699
                     /locus_tag="ACG8K3_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145503.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11696..12685
                     /locus_tag="ACG8K3_001628"
     CDS             11696..12685
                     /locus_tag="ACG8K3_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9145504.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12682..13686
                     /locus_tag="ACG8K3_001629"
     CDS             12682..13686
                     /locus_tag="ACG8K3_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9145505.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13818..14360
                     /locus_tag="ACG8K3_001630"
     CDS             13818..14360
                     /locus_tag="ACG8K3_001630"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9145506.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14380..15078
                     /locus_tag="ACG8K3_001631"
     CDS             14380..15078
                     /locus_tag="ACG8K3_001631"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9145507.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15099..15311
                     /locus_tag="ACG8K3_001632"
     CDS             15099..15311
                     /locus_tag="ACG8K3_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP9145508.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15311..16273
                     /locus_tag="ACG8K3_001633"
     CDS             15311..16273
                     /locus_tag="ACG8K3_001633"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP9145509.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16291..16686
                     /locus_tag="ACG8K3_001634"
     CDS             16291..16686
                     /locus_tag="ACG8K3_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9145510.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16849..17214
                     /locus_tag="ACG8K3_001635"
     CDS             16849..17214
                     /locus_tag="ACG8K3_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9145511.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17211..19022
                     /locus_tag="ACG8K3_001636"
     CDS             17211..19022
                     /locus_tag="ACG8K3_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP9145512.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19084..19251)
                     /locus_tag="ACG8K3_001637"
     CDS             complement(19084..19251)
                     /locus_tag="ACG8K3_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP9145513.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6228 a         3286 c         3928 g         5822 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt attctatgat
     6121 aagatgcata tgaatgaaat aaacagtaat tttatattag gatgtgaaag ttaaatgtgc
     6181 ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa caattattaa tacaatgatg
     6241 gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat atatcgataa acatgttgct
     6301 ttaggattca gaagattaag tattattgac ttagagggcg gtactcagcc tatttacaat
     6361 gaagatcgga caaagatcat tatttttaat ggcgaaatct ataattacca gcctttgaga
     6421 gaagaattga ttgcagctgg ccacgttttt caaacacatg cagatacaga agtccttttg
     6481 catggttacg aagaatgggg aacagaatta ttgcaaaaga tccgcggaat gtttgctttt
     6541 gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa
     6601 ccatattatt atgcagaaat gaacggaaca tttatgtttg gttcagaaat caaaagtttt
     6661 ctgcctcatc ctgattttaa caaagaattg aacaaagaag cgttgaaacc ttatatgact
     6721 ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa
     6781 ggccattatt atacgtataa agatggaaaa ctagacattc agcaatattg ggatgcagat
     6841 tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt
     6901 caagcttcta ttgaagcaca tacggtcagt gatgtagaag ttggttcttt cttatccagc
     6961 ggggttgatt ccagttatgt aacctctgta ttgaaaccag atcattcttt ctcaatcggg
     7021 tttgatgata aaacctataa tgaagcgatt gaagccagaa aactaaccga actattggac
     7081 ttagataata cagcagcagt gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc
     7141 cagtatcact tagatgaacc tgattcaaat ccttcttgcg tacctttgta ctttttagca
     7201 aatcttgctt cacaaagtgt gcgggtagtc caatctggag aaggggcgga tgaactgttt
     7261 gcgggatacc aaacgtatgg attccatacg aattcaaaat tcatccgtgt gattgctcaa
     7321 ggattgaaaa aattaccaaa aggaacacgc tataatttag gacgtaaaat cggaaaaatg
     7381 aaaaatttcc atggacggat ccatttatat gaatcacttg ctcctgcaaa agaatatttt
     7441 atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag aagtgttaac accaaaatat
     7501 caaacagcac catctgtgga tgaaatcatg actgttcatt acgagaaaac agaaggaatc
     7561 aaagatgaag tcaataagat gcaatatgtc gatcttcatc aatggatgcc aaaagatatc
     7621 ttgttaaagg cagataaact ttctatggca agttctttgg aagtccgagt acctttattg
     7681 gatatcgaag tcatgaaatt ggctcaacaa ataccaagta agtttttatt gaatcaaaac
     7741 aacacaaaag atattttcag acaagctgcg aacaaacatc ttccggaaga atggtcgaat
     7801 cgtgtgaaat taggattccc tgtgccaatc aaagcttggt tgaaagaaga acacggctac
     7861 gaacaagtca aagcattatt cgaagcagat tttgcaaaag aattttttga tcaagaaaaa
     7921 atcatgaagt tgctagatga ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg
     7981 acaatcttca gtttcttgac atggtataaa gtctactttg tagatgaaac gattccgcaa
     8041 gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa atatcaataa aaaagaggtt
     8101 ttctcctacc gtttaacact gagtgataag aaacaatttc aaaaaacagc tcttcccgtt
     8161 ttatagatat tatgacttat ggttcggagt tggcagagga acaccattgt ttcgaacata
     8221 agggggttgt gacacgattt tcgtcacaac ccctttacat atgttcaata atcgatttct
     8281 ttttcttttg tcccatcttt tttataaatg aacaaagttg attctttgtt ttgtgctact
     8341 tgctttcctt tttcgattgc ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc
     8401 cctttagatt tcacgatcca ttgttcttcc tcatattcta cgatcacatc tgaatcaagc
     8461 agttttccag cacgaggatt cgtatcgtgt tcatccgtct tgcttggatt tttttctttt
     8521 tcgaaagttc gtttttctga ttcactagca tcttcatacc atttttttgc ttgatcaatg
     8581 gcaattggga tcgcacggtt atcagggtac ccttcatcta ataatgcatt tgcgatgtcg
     8641 attgcttttt ttcttgtcaa tttatctaag tttttcattg atgcaggaaa gtccttcatg
     8701 ttccatggca tattagtatc cccctttatt aaaaaacata atctatataa acaagtgtaa
     8761 gggtatttat acaaaaagaa aaagattttg tttgagaagt aaaaccgttg tatttatcgt
     8821 caagtagttt tacttaacaa aatcgtgaaa aacactagca atctagaaaa aatcatggta
     8881 agataatcaa gtctgagagt catcttagag gattcttgta aaaatgggag ggaaaaacat
     8941 gcgcgtaaac atcactttag agtgtacttc ttgtaaagaa cgtaactatc taacaagcaa
     9001 aaacaaacgt aacaatcctg atcgtttgga aaaacaaaaa tattgcccac gcgaacgcaa
     9061 agttactttg caccgtgaaa caaaataatg tttttaaagt tctcaattcc ttgctggtca
     9121 aagaattgag acttttttta tctcaaaaca tctaaaatca taaggagcgg ataatatgtt
     9181 tatttccatg tgggcacaag ataaaaatgg tctgatcggc aaaaatggtt tgttaccatg
     9241 gcgattgcca aatgatatgc gtttctttcg cgaacataca atggatcgaa ttttagtgat
     9301 gggaagaaag acctatgaag gaatggggga tctttcactt ccttatcgcc atatcatcgt
     9361 gttgacaacg caaccaaatt ttcgcacgaa agaaaatgcg gaagtaatgc attcgatcga
     9421 tgaacttttg tcgtatagtc aaacgatctc agaagatatc tatgtttcgg gtggtagtcg
     9481 gatttttcaa gaacttcttc cttacactgg tcggatctgg cgaacattga tcgatagcac
     9541 gtttgaaggc gatacgtata ttgggaatat tgatttttca gacttcgccc ttatagagga
     9601 gtatgagggg ataacggatc aagaaaatct atatgcccat cgttttcaaa aatgggaaag
     9661 agtaaaaaat agggttgctt caaaagaaga ataaaatcta tgcgtctagt ttgttaataa
     9721 gaaaaaaagc atctcacgac taatcgtgaa gattcttttt ttagttaaaa tcaatttagt
     9781 ttttctttga tatcattgac catttcctga ctggcaatag gtccgttata tagccaacta
     9841 gattcaggtc cgaattgcag tacatgattg tctgtcactg ctttaagatt cttccagctt
     9901 ggttcattga acatggcagc atctttgtca ctgttcacaa ggatgatgta gtcagcatct
     9961 aattcagcta gcttttcaga ggaaacagca gaccagtcag aagtggcttt tttggagacc
    10021 tcttccacta gatcaggaac accaaattcc agatcgtcgt atacgattct tccacttgac
    10081 cgatttttgc tgaccataaa ggctgagttg ttggtcaccc acaacactgc agctgtcttg
    10141 ttctcgattt tatctgctag ttcttctcga gttttcttga tggatttttg ataatcggaa
    10201 atgatttttt ctgcctcttt ttcttttgcc aaagctttgc cgacatcttt tagttgttct
    10261 tcccatgtga catctgttcc gttttttaca acataagttg gcgcgatttt gctatattgt
    10321 tcgtatttcc cgccttcgac agtagcggaa gaagaaatca acagcaaatc aggctcaaag
    10381 cttaacactt tctcataagg aaggtcataa gaaatcgttg ggacatcctt taaatcatct
    10441 tgcagataat cttgaatgga accactgcca acagtccact gggcgatagg tttttcccct
    10501 agtgcaatca gatagtcttc taagtaactg ccgatgatcc gtttgggcgc gtttggaatc
    10561 tctactttat gtccaagtgt atctgtgact gtatgagtac tattggatga agtcgtcgaa
    10621 ttggcagttg tagctgtttc tttatctgaa gagggggagc aagcgctcag tactccagcc
    10681 agcagcaata tgctagataa tgtgaatact tttgttttta atttcatttc tttcctccta
    10741 acataattga gaatctttct cattggttat tattcctgat atttcgaacg ctgtcaaccg
    10801 cttcatttgg ctatagatat gaaagtagcg cttgacgaaa tgttgaaaca gcgtcataat
    10861 tgagaatgat tttcagcgaa aggatgggtg aaatgacgag attaagagca gctgacattg
    10921 cagccggcta cgaaaacaaa aaaatcattg aaaacatgtc tgttacgatt cctgaaggga
    10981 aaatcactag tttgattggg ccaaatggaa gtggaaaatc tactttattg aaaagtttta
    11041 cccgtatact acctgtgaaa tcaggaaaga tcattgtcaa taactctgat atctctacat
    11101 acagttcaaa agaacttgca aaaaaaatta cattattagc acaaactagt gaacacccta
    11161 caggtatgac agtagaagaa gtagtatctt acggacgata cccttatcaa aaactgttca
    11221 gtggcatgga cacgcaagat catgaagcaa tcgagtgggc gttggatgct acaggtctag
    11281 ctggatttcg tatgcgggat atcgcaacgc tgtctggtgg acaaacccag cgtgtctgga
    11341 tcgctatggc gcttgctcag gaaacagata tcttgatctt ggatgaaccg acaacatttt
    11401 tagatccggc acaccaatta gagatcctac agttattgac tgaaatcaat caaatgaaac
    11461 aaacaacgat cttgatgtcg attcatgaca ttaatcatgc ttcacgtttt tcagattatt
    11521 tattagcaat gaaaaacggt aagttgatca cagaaggtac acctgaagaa gtgatcacaa
    11581 aagagtgtat gaaaaaaata tttgagattg atgcaacgat tgtcacgctt ccaagaacaa
    11641 ataaaccagt ttttctaacc tatgatttgt tgccaaaaga gcaggaggat caattatgag
    11701 gactatgaag aaaaatagac tcctgctttt ggtctgtttt gttcttttga ttattttttt
    11761 tgttgctctt tcaaatggcg cagcgactgt gggaaaagca gatttttggc aggcgttgtt
    11821 tgattttgat agatcaaaac aaagtcacca aatcatacga aatattcgtt tgcctcgtgt
    11881 ggcgggagca tttcttgtag ggagttgttt tgcattgagc ggtgcattga tgcaaggagt
    11941 gacaagaaat cctttagcag attcaggctt actaggaatc aacgcgggtg cttcgttggc
    12001 aatggccctt agttttgctt tttggcctaa agcttctgat ttttctgtgt ttctaatgtc
    12061 attggcaggt tcttttgctg taactttgct cgtatttggt tttttgcagt tttcgaaaat
    12121 gggcatgaat cctttacagc tgattttagc tggcgtagct attagttctt tttttactgc
    12181 tatcagccaa gcacttaccc aattctttaa tctgcagcag gatcttgctt tttggtttgt
    12241 aggcggagct gccaatatca catggaaaca attagcagta ttgtttcctt tatatatact
    12301 tgctgttttg gtttcttttt cattaggaaa agcaatatct ttgattcatt tgagtgaaac
    12361 acatgcatta gctcttggaa gtcatcctaa acgaacgcgg ttgattgttt tctgtctagt
    12421 tgcagtgtta gcagccattg ccgtttcgtt agtcggacct gtttcattta ttgggctgtt
    12481 ggttccgcca gttgttcgcg gaatggttgg aacggactat cgttatgttc tacctgtctc
    12541 ttttttagtt ggcggtggat tggttatgtt ggcggatttt acagcgagga tgatcaatcc
    12601 cccttttgaa acaccttttg gattagtcat tgcattgatt ggcgttccgt ttttactgat
    12661 acaagtaagg agggaacaac ggtgaaaaaa atatattgga cgttctattt cttgcttttt
    12721 gtacttgtca tcctgtctat aggtagttta atgattggta cgccttttat ttacctggat
    12781 cagctttttg ccacgttcat agggaaagaa tcatcttctt tggcgctgat tgttagagaa
    12841 tttcgagttc ctcgattact gatcagttta ttagcaggag cttgtcttgg cgtttctggt
    12901 tatatcttgc aaggagtaac aagaaatgaa ttggcagatt ctagtatatt gggcattaat
    12961 gccggagcag gatttttagt gatgctttat ttaggtttct tttcgcacta ttcgctgccg
    13021 tttctattgc tagttgtagc ctttctaggt ggtgttttag ctgcttgctg tgtctatctt
    13081 gcggcctatg atcgaaatgg ctttttgggt atgaatcgaa tcttattgtc cggtattgcc
    13141 gttaatgccg gcctttctgc attaacattg ctatgcacga tccaattatc taaggaaaat
    13201 tatggatttg tgaatgcatg gttagcggga tcaatctggg gtgcgagctg gccttatgtt
    13261 tgggcattgc ttccatgggc ggttatctta attccgctag gaggacttta tgcacagagg
    13321 attggattgc tatcatttgg tcaagaacgc gcgcaaggat taggccttaa cgtggctaaa
    13381 agtcaaaaaa tccttttgtt gttagctgtt gctttggcaa gcggtagtgt agcggtgaca
    13441 ggtagtttat cttttgtagg actactagct cctcatgctg cgaagctagt cgtacgtaaa
    13501 gaaaataact ggacctttgt tctgagcggt ttgttcggtt ccatttttct gcttagtgca
    13561 gatatggttg ctagagtgat tctaccaagt ggggaagtac ctacaggaat tttgattgcc
    13621 ctttttggtg cgccatattt tctgtatgca ctttttatca gacaaaaaca cgcgctgaac
    13681 agttgaaatt tattaaaaaa ggaaggccaa tcatttctag gtcttctttt tattctattt
    13741 atgcatttat ttgaaagttc gattgctatt tttctttttt tcctatacca ttttatgaga
    13801 gaaggaaggg tgagtttttg gaccaattga aagtaagatt acgccggaaa ggaatcgaaa
    13861 agctgaaaaa tctggccaaa agtggcaaga aaaaggaaaa agaacagcga atctataaac
    13921 agctattcgc cagcgagtac tggaagtcag caaaagtgat agggacgact ttatcgaatg
    13981 aatttgaact ggatacacag ccggttatcc aacgagcaga aaaagaacaa aagaaaatag
    14041 ttgtacctaa aactttgccg aaacgtcaga tggcgtttta tgaaatggat gaacagtccg
    14101 ttcttgtacg ttcgagtttt ggtgttcttg agccacaatc agagaagctc tatttgccgg
    14161 agatgatcga tctcctcatt gtaccaggta ttatctacaa tagagccggt tatcgaatcg
    14221 gttttggcgg tggttattac gaccggtatt taaataattt tcataaaaaa acatgtagcc
    14281 ttgtttttcg tgaacaattg aatgaacaat ggcaaccgga gagctttgat cagaagatcc
    14341 aacaattgtt tattgactaa aatgaaatgg gaggttttca tgaattatca acagcaaata
    14401 aaattgcgtg catggttaag acgaccgtta tggacctatg cttttttagg gatacagaca
    14461 gcggtcttcc tcatgatgga attatttccg cgacttgaga tcccttatta tgccggaatg
    14521 tatggtcctt atcttgttca cttcaatgaa tggtggcgtt tagtgacacc aatcttcatc
    14581 cattttggac tgatgcattt cgtaatgaac tcactgatac tgtattttat ggggcagcaa
    14641 atcgaggcaa tctatggcca ttggcgtttt ttcttgattt atatgttcag cgggataatg
    14701 ggaaatacag caagctttgc ttttaacgaa gcgaatgttc tgtctggtgg tgcgagtact
    14761 tcgatattcg gtttgttcgg tgcgttgttt atcttagggt ttcattttaa acataaccca
    14821 gcgatccagc agctagtcag acattttctt ttatttattg tgttgacatt tgtttttggt
    14881 cttctggata cctctgtaga tgtttgggga catgtcggtg ggctaattgg cggattagtg
    14941 ttagggaatg ttttgggtct accgaaacaa cagacaagct attcgataca ccaaaggatt
    15001 ctttctacac tagtttttgt atttcttttt gtaatatgta ttttactggg tttaaaaaaa
    15061 tatggtttac ttgtataata tgaaggaagt gcttaaagat ggagagctta tatgatgtac
    15121 agcagctgtt caaaaaattt ggcatcttta tctatgtggg tgcacgcatc tatgacattg
    15181 aattaatgac tatcgaattg cgtaatctgt atgatagccg tttgattgat catgatacat
    15241 acatgacagc gtggcgcata ttaaaaagag agcaccgaat tgaagaaagc cgaaagaaag
    15301 gaaatttgta atggacaaga agattattgg gattgattta ggtggaacaa cagcaaaatt
    15361 tgcaatttta acaccagaag gagagattca acaaaaatgg agcatcgata ccaatatttt
    15421 ggatgatggt aagcatatcg ttcctgaaat cattgaatca atcaaccatc gtttgaattt
    15481 atataatatg aaagccgaag actttatcgg aatcggcatg ggtaccccag gaagcgtgga
    15541 cagcgaagct ggaacggtta ttggtgcgta taacttaaac tggacagaag ttcaatttgt
    15601 gaaaaaactg atcgaggcag gaacaggaat caaattcgct atcgataatg atgcgaatgt
    15661 tgctgcttta ggtgaacgat ggaaaggtgc tggagaaaac gatccagacg ttgtattcgt
    15721 tactctagga acaggtgttg gcggtggtat cgttgctggc ggaaatttga ttcacggcgt
    15781 gagcggagca ggcggagaga tcggccatat cactgtggat cctgaagggt tcgaatgtac
    15841 ttgcggtaaa cgcggatgtt tagaaactgt gtctagcgct actggtgtgg tacgtctagg
    15901 tcgttacttg gcagaagaat acgcaggaga ttcaaaactg aaagcaatgt tagacaacgg
    15961 tgaagaagta acaagtaaag atatctttga aatggctcaa gaagacgatc cgtttgcatt
    16021 gatggttgtt gatcgcgtat gcttctactt aggtttagct tgcgccaact tggggaatac
    16081 attgaatccg tcaagtattg tgttaggtgg aggagtatcg gcagcaggag aatttttacg
    16141 tagccgtgta gagaaatatt ttaaagaatt tactttcccg caagtaagag aatcaacaaa
    16201 aatcaaatta gcagagcttg gtaatgaagc tggtgtcatt ggtgctgcct cattagcctt
    16261 gaaatttgct taaaaaaacg gaggcattac atggttttat gggtaattaa tatcattttg
    16321 ctattgattg tgcttgcaat catcttttgg gaagtctatc tacgagtaat ggccaagcgt
    16381 tcagcaacga ctttgacaga agaagaattt cgagaaggaa tgagaaaagc acaagtaatc
    16441 gatgttcgcg aaaaagatgt gtttgatgct gggcatattt taggtgcaag aaacatccct
    16501 tacactgttt tgaaagattc attaggatct attcgaaaag accagcctgt ctatatttac
    16561 gatcaaaaga aaagcttgag catccgtaca gcaaataaat tacgtaaagc aggttatcaa
    16621 gagatttatt tacttaaggg tggatatgac ggatggtctg gaaaaatcaa aagtaaaaaa
    16681 gtataaaaca gatgattgta tgacagcttt aaaaaccgaa tcaatcatta gattttgacc
    16741 aagatcacat gaacggcgca atggatagtt gtgccgttca ttttttttat tttgtgctat
    16801 tatctacata tataagctaa gcgaaaaggc agaaagaagg aaacaactat gatcaaatta
    16861 aaacgtgttt accttgatgc tgaaaaagag gatggctata gaatactcgt ggataggttc
    16921 tggccacgag gaatttcaaa agaaaaagcg cagttagatc tgtggttgaa agaaattgca
    16981 cctagtaatg aagcacgcaa aacttttgac catgtaccag aacgatttgc ttcatttgca
    17041 aagaaatacc agcaagaatt gattttcgaa gaagaaaaga agcaagctat ttttaaactc
    17101 agtcaactta tgaaagaaca tccagtcatt acttttgttt atggtgcaaa aaatgaacat
    17161 cagaaccatg ctgttttatt gaaaaaattt atcgaaatgg aggcatgcaa atgaaaatag
    17221 ggatcattgg tgccggacct agaggattaa gtatagcaga aaggattctc cataataatc
    17281 aggacagaca ttctcttgaa ctttttcttt tcgatcccaa tggtcctgga ggtcgagttt
    17341 ggcggttgaa tcagccaccg gagttattaa tgaattctgt ttctcaacaa gtgacgcttt
    17401 ttacagatga aacattgtcc agtggaggat tggtaacaca gggacccaac ttgtttcaat
    17461 ggagtaaagg acctgctaca gattatatcg aacagacggt aaccaaaaat aagcaactaa
    17521 ttatagaaga aatcaatcgt ctggaaaaag atcagcaatc tactcgatgt ttatacggac
    17581 tctatcaacg ctggtttttc tcaaagttgc aagaagaatt tccagcttct attcagtttg
    17641 tttattcttt agtaaaaaaa gtcagcaaaa gaaaagatgg ttatctttta cagacagatc
    17701 agcaaagcta ttctatcgat cagcttgttt tagcaagcgg tcactgggaa aatgatgaga
    17761 cagaagaaga aagagtgtgg gtagactacg caaagaaaaa tgatttactg tatcagcttc
    17821 cttctaatcc tgcagatgtc gatctttcga agataccgcc taaagaaaat gtctttttaa
    17881 gaggtctagg cttaagtttt tttgattatg tcggattgtt tacagtatca agagaagggc
    17941 gcttcgagaa cgaaaaagga aaacttgtct atcatccatc aggcaaggaa ccgattgtat
    18001 acagtggatc aagaaggggg ctcccttatt accctcgagg aagaaaccaa aagcaaggcg
    18061 gatcaatggc atggccacgg ttactaacaa aagaaaacct ggagcaatgg catcagtcgg
    18121 gatttttgac tggcgaaaca ttttttgact atctgaaaaa agacgttgag ttgttttatt
    18181 acaagaaaat catcgaagaa tatcatttgc cgattccaaa gaaaaatttt gagcatgatt
    18241 ttctctccct ctcttcagag gagtgtctcg gaaagcatcc agcactaatc ccttatagat
    18301 gggattggtc gtatttagaa attcctctta cctatcagga tgagtccttt gcagaagcta
    18361 gccgagcatt tattgaaggg cagataaaag aagcggaaaa aggcaattgc acaggagcat
    18421 ttgcttcaac atttgatgca ttaaaagact ggcgtgatcc aatccggcag gctattgaat
    18481 ggcacgtttt ttctgcaaag gaatacaaag aaatactatg gggatggttt actcatctaa
    18541 atagcttttt aacgatcggt ccgccagtca ttcggacgaa agaattagca gcactgatag
    18601 atgcgggtat ttttcatttg ttcgaaccgc catttgatat tcagcaaaag cacaaccgct
    18661 atgttatttc tctacaagat aaagaaatca gcagtcgcta tttgattgaa gctcgattgc
    18721 cacaaacaag cttttctcgt tcaagtaatc ctgtcataaa aaatttgaag gaaaacaaac
    18781 ttgttcgccc tttttcattt tcagataata caggaagtta tcattcagaa gctatagatg
    18841 ttgagcgagg tacgaatcgc gtgattgatg cttcagggga aatccagtct gacttttatt
    18901 gctatggaat acctgttgaa ggtgtggatt ggctaactgc agcagtagct agaccttata
    18961 cagatccatg gaatctgaga caggcagata aaatagcaca acttatattg actgacaaat
    19021 aaaaatacgc atgatgactt atctgttctt tcacaagtct tcatgcgtgt ttttaaataa
    19081 ttattaacga gaaacgcgtt tgcgcattgc ttttttacgg ttttcttcga tcattgcttc
    19141 tttttcgtca attggatcga tcactgtttt tttcacagaa gcgaccaaac cagcaacggc
    19201 agcggctgtt gccagacttc ctactaatat tccagataca aattttttca ttgaatccac
    19261 ctca
//
LOCUS       DAZKCN010000050        17734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000050 DAZKCN010000000
VERSION     DAZKCN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.051"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1325)
                     /locus_tag="ACG8K3_001638"
     CDS             complement(24..1325)
                     /locus_tag="ACG8K3_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9145514.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1555..3546)
                     /locus_tag="ACG8K3_001639"
     CDS             complement(1555..3546)
                     /locus_tag="ACG8K3_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP9145515.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3690..3956)
                     /locus_tag="ACG8K3_001640"
     CDS             complement(3690..3956)
                     /locus_tag="ACG8K3_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9145516.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4466..6592)
                     /gene="pnp"
                     /locus_tag="ACG8K3_001641"
     CDS             complement(4466..6592)
                     /gene="pnp"
                     /locus_tag="ACG8K3_001641"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9145517.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6780..7049)
                     /gene="rpsO"
                     /locus_tag="ACG8K3_001642"
     CDS             complement(6780..7049)
                     /gene="rpsO"
                     /locus_tag="ACG8K3_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9145518.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7300..7863
                     /gene="def"
                     /locus_tag="ACG8K3_001643"
     CDS             7300..7863
                     /gene="def"
                     /locus_tag="ACG8K3_001643"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9145519.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8116..8637)
                     /locus_tag="ACG8K3_001644"
     CDS             complement(8116..8637)
                     /locus_tag="ACG8K3_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP9145520.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8640..9482)
                     /locus_tag="ACG8K3_001645"
     CDS             complement(8640..9482)
                     /locus_tag="ACG8K3_001645"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP9145521.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9475..10200)
                     /locus_tag="ACG8K3_001646"
     CDS             complement(9475..10200)
                     /locus_tag="ACG8K3_001646"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP9145522.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10376..10672
                     /locus_tag="ACG8K3_001647"
     CDS             10376..10672
                     /locus_tag="ACG8K3_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145523.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      10928..11102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            11240..12832
                     /locus_tag="ACG8K3_001648"
     CDS             11240..12832
                     /locus_tag="ACG8K3_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9145524.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            12845..13585
                     /locus_tag="ACG8K3_001649"
     CDS             12845..13585
                     /locus_tag="ACG8K3_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9145525.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            13753..15102
                     /locus_tag="ACG8K3_001650"
     CDS             13753..15102
                     /locus_tag="ACG8K3_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9145526.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(15230..15820)
                     /locus_tag="ACG8K3_001651"
     CDS             complement(15230..15820)
                     /locus_tag="ACG8K3_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9145527.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            15967..17319
                     /gene="brnQ"
                     /locus_tag="ACG8K3_001652"
     CDS             15967..17319
                     /gene="brnQ"
                     /locus_tag="ACG8K3_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP9145528.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         5091 a         3595 c         3396 g         5652 t
ORIGIN      
        1 aaaatgtcac agcctttttt cttttattct atttttcttt ccgtggactc tactttgaaa
       61 taatcgatat gtgcatgctg gtattctaat accgtgaaac ggaatgatgc aatctcaatg
      121 attgttcctg cttttatttc tttatatcgg ctagaagcaa agcccgcaag tgtagtaaaa
      181 ttcgtttctt caaactctgg aatctctaca tgaaaataac gctggaaatc atctaatgtt
      241 agtttgccgg agatgatata agagccatct gactgttccg ctatcaatgc ttcgtccaaa
      301 tctgtctcat ctcggatttc cccgacgatt tcttctaaaa tatcttcaat cgtcactaag
      361 ccgcttgtcc ctccgtattc atcttttaag atagcaatat gctttccctt ttttcgcatg
      421 atcgtcaata atttttttat agggatcgtt tcagttgtta cgatcggctc ttcaatcaac
      481 tgttctactg tcagctggtc atctttataa gattgcttga tcaaatctgg caaggtgaca
      541 taacctaaaa cattgtcttt tgattctaag ataaccggat agcgagtatg tccttgttca
      601 atcgaagctt ttatcgcttc tttgacggtt tctgaagctt cgaatacttg catggacgta
      661 cggtctactt gtatatcttt tgccaaggta tcatcaaatt caaacacgtt ttccaaataa
      721 tgatattctt ctttggtcaa ctctcctttt tcataagaat tcaatgcgac taatgtcagc
      781 tcttcttgag acaatgtctc ttctccttca gatacgagac gcacacctaa tagcttccct
      841 agccctgcag ctgaatgatt caatacccaa ataaaaggaa acattacttt ataaaaataa
      901 tgaagtggtt taactacgaa caagacgacc ttctccgtat tgacgatact atagctctta
      961 ggaagcagtt cgccaataac cacatcgata tacgtaacta aaataaatcc cagcgccaca
     1021 gagattgtac tgctaatagc cgaactaaaa ggtagtttac cgatcactgg ttctagtaaa
     1081 gccgcgacag atccttcacc gacccacccg atgatcaaac cggttagcgt aattcctaat
     1141 tgactagctg ataaatagtt atctaaatgg tgtgtaacat gaagagctag cttggcgttc
     1201 tttacatttt ccttttctag ttgttccaat cttgatcgtc tgattttcac taaagcaaat
     1261 tcacttgcta caaaaaatga agtaattgca atcatcaaaa ataaaataat gaaattgata
     1321 accatattct cccgcctttc tctttttcta ttgtaccctt tttctacaaa aagattgaaa
     1381 ataatgcgta ttcatattta ggaataaaaa aggagatgcg gcatttttca gccacatctc
     1441 cttatcactt caaaatcatt gcgttacttg ttgctaaaca gctttttcat gacctctgat
     1501 aaacggtgtt caagtagctg acattcgaca ttagttcagg tcaaagcgct actttcacaa
     1561 ccgctctttc aaccatgttt tccgagtatt tccaatgata agcggaacag tttcatgttt
     1621 tacttcgctg tactctagcc caaaccatgt ttctggcgag gtagcgattt ttttgattgc
     1681 cagttttgcc tgcatgattt gacgttgcca ttttcctaga gttgtcacat tggaaactac
     1741 ttcatcaatc aggacaaatt ggaaatcgcc aacttctcgc cctggtgtca gagagtatct
     1801 ttgtggctgt tttggcaaac ggccttgttt catcaaatca tggatgactt ggcgaacata
     1861 tacgttgatt tcctgttgga ccctgaaacc taagaacaga ttcacttgga caataaaatc
     1921 agtgtccatc atatctactt gatattcttt cgtatatggc tcatccgtca cttccacatg
     1981 aacaaaccaa tagactcgtg ctcttttagg ccgtttatct aaaatcgaat agatgaactg
     2041 tcggccaact ttatcttcag ccatatcagg aaccaagaag accacatttg tctgatagat
     2101 tggtaaagaa gtatcatcac gtaattctct aagctgcggg atataatctc tcaaagacac
     2161 atcctcggtt gacgattcgc gaataatgtt cccttgttcc caaataaaca tcacgactaa
     2221 gatgatcaaa ccaatcagaa cggctacaaa accgcctcgg aagaatttga caacacttga
     2281 gataaagaaa acgacttcta tcgcaccgaa gaaaaaggta actaaatatg caagccaacg
     2341 agaatgccca ttttggataa gataaaagta aagcagtgct gttgtcatca acatcgtcac
     2401 agtgatcgat agaccgtaag cagcttccat atgatgagac gttcggaatg tgacaaccac
     2461 tagagaacag gcgatccata aaatcagatt gactgctggg atatacattt gcccgatact
     2521 gttcccagga tacatgatct ttaaccgagg cagcagtttc aacttgattg cttctgaaac
     2581 tagcgtaaac gaacctgtaa tcaatgcttg cgaagcaatg actgcagcga ctgttgcaaa
     2641 agcaacgccg atcacgatcc aactttgagg catcatttgg aagaatggat tcaggttctt
     2701 gatttgctgt gcttcagggt tttgataaac attcaacaac cacgcagcct gcccaaaata
     2761 attgataacc aaacaaattt tgacgtaagg ccagctcaga cgaatatttc gtttgccgac
     2821 atgtccaaga tcggaataaa gcgcctctgc acctgtcgta gccaggaaaa tatttccaag
     2881 aatgaacaag cctaatttgt tatctgcact aaaaagcagc tggatagcat aataaggatt
     2941 cagtgcacgg ataactgtcc agtcttgact gaagttcatt aatcccatta cgcctaaaaa
     3001 agtaaaccaa gcgaacatga ttggtccgaa tgcttttccg acaatatctg taccaaagcg
     3061 ttggaccatg aataaaacaa aaataataac taaagtgata atgacgatca cgttctggtt
     3121 attaccgaac cggtcaaaaa actgcggaat tcctcgcaag ccttcaatcg cagtcgttac
     3181 cgtcacagca ggggtcaata ccccatctgc tagtaaggca gctcctccga tcattgcagg
     3241 aatgatcaaa tatttcccac cttttcggac aagtgtgtag agtgagaaaa ttccaccttc
     3301 accatggtta tctgcattca atgcaatcac gacatatttg attgttgtca gtagagtcaa
     3361 tgtccaaaaa atcaatgaga ctgatccgat aacaaaatca ggattgattc ctgacaatcc
     3421 gccattatct tcaacaatcg atttcataac gtaaagcgga cttgtcccga tgtctccgta
     3481 gacgactccc atcgctacaa gcaggccagc cattgatatc tttttggtat tgtgcgttgt
     3541 tgccacgttt ttccctcatt tcttgttata tggatatcct ttaggtaagc ttgaatgata
     3601 gatgatattc ttatccatga cacccgcttt cctaaattgc ttctaattat ccaagtaaca
     3661 cactgaagtg tcaacttatt tgaataaaat tattctttct ttggacgttc tctgatacgt
     3721 cttgccacaa atgaataaag agcaaggcaa aaaattgcac ccaataatgc tggtatcaaa
     3781 taaaaaccgc cgagttccgg tcccagtggt cctagtaaaa attcgccgag ccaactacct
     3841 aaaaaaccga taatgatatt tccagatacg cctcccggga cttcctttcc taaaattgcg
     3901 ccagctatca aacctaatat accgccgata aataacgacc ataaaaaatt cagcatctcc
     3961 tttccctcct ttatccatat ttctcacttt tattttacgc tttattcaga aggactccaa
     4021 aaatatgcgc caagaaactc cagaaaaaaa gaagttgtaa caaaagtgtt cagcttattt
     4081 ccgtagaagc tgcttctgga acaccgtgaa tcagaggttg tgaaatcagc ggtttgacct
     4141 gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat
     4201 atcgagggac agctgattga acatcgttta ttggagaggt tgtaaaaaag ctgttctgca
     4261 tcaagtaata agaacagcca agtaaaaaca gctcctaatg tttttaactt ggctggactt
     4321 attaccgcag atgcaagctt ttgaacaccg tttattcgaa aaaaaaggac tgtgacacgg
     4381 cttttgtcac agtccccttt tttcgaataa ttattgtccc cctgtcacct tcacgatcac
     4441 aagtacatta ttcctatgaa ttaaattatt tttcttcatt tttcgttgaa ccatcttcgt
     4501 tcaacattgc ttttcttgac agatttacgc gtccttgttt atcgatttca gttactttca
     4561 caaggatctc atcacctagt ttaacaacat cttcgacttt attgacacga tcgtttgcta
     4621 attgggaaat atggacaagt ccatcttttc ctttgatcaa gttgacgaat gcaccgaatt
     4681 tttcgatacg gacaactttc cctagataaa cttcaccaac tttgacttct ttggtcaaat
     4741 cttcgatgat cttgatagct tttttgatca tttctgcatc agctgaagca atacttactt
     4801 ttccatcttg atcgatatcg attttcacac cagtttcatc gatgattccg ttgatcgtat
     4861 cgccgccttt accgataaca tctttgattt tagcaggttc gatctggatc atttcgattt
     4921 ttggtgcata ttggcttaat tcttcacgtg gagcagcaat cgttgaagtc aattcttcta
     4981 agatttccat acgtgcttgt tttgcttgag ttaatgcttc agttaggatt tgttctgtga
     5041 ttccttgaat cttgatatcc atctgtaaag cagtgatacc atcttttgta ccggcaactt
     5101 taaagtccat atcgccaaga tgatcttcca gcccttggat atcagttagg atcgtatagt
     5161 tttcaccatc agaaacaagt cccatcgcaa taccggcgac tggcgctttg attggtacac
     5221 ctgcatccat caatgctaat gtgccagcac agatacttgc ttgggaagaa gaaccatttg
     5281 attccaatac ttctgctact aaacggatcg tataaggaaa ttcttcttct gaagggatta
     5341 cttgtgctaa cgcacgttca cctaatgcgc catgtccgat ttcccgacgc cctggagagc
     5401 ctgcacgtcc tgtagaacca acagaaaatt gagggaaatt gtagtggtgg atgaatcgtt
     5461 tgctgacttc cacccctaaa ccatcaatga tttggtgctc tcctagaggt gcgagtgtac
     5521 aagctgataa agcttgtgtt tgtcctcgtg tgaacaaacc tgatccatgt acacgtggca
     5581 ataagctgac ctctgatgac aatggacgga tttcatccaa tttacgcccg tcaggacgga
     5641 tcttatcgat ggtgatcaat tcacgtacga catctttttc aagatcttct gtgatttgtt
     5701 taacttcttt caacaagtga tcttcatctt catgacctgc aaatttctca gcataagctt
     5761 cgcggatatc gatcttcacc tgctcgatat tatcttcgcg agcaagtttt tcttcagtca
     5821 ttactgctgc tttcattgta gcgtaagaag catcaaagat ttcttttttc aatacttcat
     5881 caacttgcag taaagtcact tccatttttt ctttccctac tgcttgaacg atttcttctt
     5941 ggaaagcaac taactctttg atagcgtcaa aaccaaacag caatgcacca agcatgtctt
     6001 cttctgaaac ttctttcgcg ccgctttcaa ccatattgat ggcttgtttc gttccggcaa
     6061 cagtcaattc gatatctgtt tgttctgctt gttctactgt tggattcaag acgtactcgc
     6121 catttacacg accaacttct acgcctgcga ttgggccgtc aaatgggata tctgagattg
     6181 atagtgctaa agaagaacct agcattgctg ccatagctgg cgagcagtct gtttctacac
     6241 tcatcacgat gttcgttact tggacttcat tacggaagcc ttctgcaaac atcgggcgaa
     6301 ttggacggtc gatcaaacgg gcagtcaatg ttgcttccgt acttggacgg ccttctcgtt
     6361 tgatgaaacc tccagggatt ttccctgctg catacatttt ttcttcataa ttgatcgtta
     6421 atgggaaaaa gtctgtatct tttgcttctt tagaggccac agctgcactt aagactactg
     6481 tatctccata acggactaaa acggctccgt ttgcttgttt tgctagctga ccgacttcga
     6541 tttgtaaggg acggccgccc catgtcgttt tgaatacttg tttttctgac ataacttctc
     6601 cttttccttc tcgtgaaagc aagacgctac taaacaaatg aaaactgccg aaagccgtct
     6661 tttgagcagc tttcatttgg ttagtaggag tccatctagc ttgatcacgg aattttactt
     6721 aattgatata cgagtaaaaa aagtgagatt catgtgaatc ccactctttt tgactaaaat
     6781 taacgacgca agcctaaacg tttgatcaat tcgcggtaac gttgaacgtc agttttacgt
     6841 aagtaagcta acaagttacg gcggtgacca acttttttca ttaggccgcg gtatgaatga
     6901 tggtcttttt tgtgcacgcg tgcatgttca ttcaagtggt tgatttcttc agttaataca
     6961 gcgatttgta cttctggaga accagtatcc ccttcatggc gagcgtattc gttgatgatt
     7021 tcattcttgc gttcttttga aattgccatt tctttcacct ctttcgatta ttcccctgcg
     7081 actgagtaaa acgttggtga ttcgcttaac caagtggcag gtcatgtgcc tagagcacac
     7141 agctatactt tacctgaaac cgggttgaaa atcaagtagt taatcattaa gaaaaggtag
     7201 ttttcaaaca atcttactac tacttttcca tttttgtggt aaactataat gcgaaaatag
     7261 acttgaaaca cagtctttta agaaaggggt acatattcca tgattacaat ggatgatatc
     7321 attcgtgaag gaaatccgac cttacgcgaa gttgcaaaag aagtttctct tccattaagc
     7381 gaagaagata tctctttagg aaaagagatg cttgagttct taaaaaacag ccaagatcca
     7441 atcaaagctg aagaactaca cttacgaggc ggtgttggct tagctgctcc tcaattagat
     7501 atttctaaaa gaatcattgc tgttcacgta ccaagtcctg acccagaggc agatggacct
     7561 tctctaagta ctgttatgta taatcctaaa attttgagcc attctgttca agatgcttgt
     7621 ttaggggaag gtgaaggctg tttatctgtt gatcgagaag taccaggata tgttgttcgc
     7681 catgccaaaa tcactgtgtc ttattatgat atgaatggtg aaaaacacaa gattcgtttg
     7741 aagaattatg aatcgatcgt cgttcaacat gaaatcgacc atatcaacgg tgtgatgttc
     7801 tatgaccaca tcaacgatca aaatccattt gcactaaaag aaggcgtatt ggtgatcgag
     7861 tagagaattg gcaggttcct tcgatactat gctacaagaa agctttataa aaaaggggag
     7921 tgctcaaaag ctgcacttcc cttttttatg tttctccact aatcgaaatt tagatagaca
     7981 tctagaaatc gcaattccaa aaaattctgt gtattggata cgcatctttt cttatttttt
     8041 cttatttctt ccatatgggt gtttatctca gagatgatag aatagtttct aggataaaaa
     8101 gtagcagcta cgatgttatc cttcgtagag gattcctgct tcagctagtt tttgtttgat
     8161 gtttttcagc atatgctttt ctgggtagcg gatcgtatgc agatggatgc cgcctgttaa
     8221 ttcggataac aaagtagcat tacttttttg ataacgtctc ataaaggaat cgatatcttg
     8281 tggcgttttg atgtgcaaag ttcctgctaa ctcaccgtaa aggggatgtt ccacaattac
     8341 atcaatgatt tcaccaccat tttcaacgat tagtctcagt tcttcttctg tttgatcttt
     8401 tccatgctgg acagcgattt tactgatgtg cccagtattg gtttcttctg tttcgaggat
     8461 ataacctctt gctgttgcca cgatactttt acctgcagct cgtagtaaag caatatcgcc
     8521 tacaatgatc tgacggctga ctccaaaagt tttcgcaaac ttgctagcac tcactggttt
     8581 ttctgtattt gccaaggttt gcatgatttg ttttcgccgt gtctctcctt cgatcacact
     8641 tattcctcct tcattttgtt ttggtattcg cgcgagtgtt tcatcaaatc aacatacgtg
     8701 tctgttattg tttccttata tcccttattt ttcagcagac taacaacttt atccaatact
     8761 tgctgctctc tttttgcatc ataaactgct tggttttctt cttttttcac ttgtcctatt
     8821 ttagttaccg tatccattct tttttctaat aaagcaacca actctcgatc gatcatatcg
     8881 atcttatgac gtaatgtttc aagagactct tctgttagtt cttcctctac tgtcttgatg
     8941 atacgagcag gatttcctgc aatcactaca ttgtcgggaa aagatttggt cacaaccgaa
     9001 cctgcgccga ctactacgtt gtcccctaga gtgacacccg gcgtgacgat aactcctccg
     9061 cctaaccaaa cattattacc gattttaatg ggttttgcgt actccagacc gctgttacgt
     9121 ttcactggat gtagtggatg cgtcgccgtg taaagctgga cattcggagc gaacatgcag
     9181 ttatcaccga tttcaatcgt gctgacatct aaaaatgtac aattgaaatt tgcataaaaa
     9241 ttctccccaa caaatatatt atatccgtaa tcaaaattga taactggctc catatagatt
     9301 tttttccctg tacggccaaa agtctctttc aacaattggg atcgcagttc cgctgattct
     9361 gcttggttga tgatttggct ttgacttctt gcaaacatac ggtcggcaac cagctcagga
     9421 tcattggcaa aataaagctc gcctgcaatc attttttctt tttcggtttt gaattcagtc
     9481 ataatctgct cctaattctt gtattttttt gaaaacaacc gctccgtctt cttgaataaa
     9541 cgtataaggc tccgctaaat aaacaggcgt ttgattgatt actaagattt tggctgacgg
     9601 ctggcgaaac tgtaccagat cacaaaacgg atgaacttga aaactcgttc caacaatgac
     9661 gatcagctct gcttggctga cggcataagc cgcctgctcc atgactccat cttgaaaccc
     9721 ttcaccatat aacacgatgt ctgggcgtat ttgtcctccg catgcttgat gtttgtcgct
     9781 gtgaagatat tctttccagt ctaccgtttg ttgacatttt cgacaatgac attgatacag
     9841 actcccatga aaattgacga gttgcgggct tttagccttt gcgtgcaaac cgtcgatgtt
     9901 ttgggaaaca acccaaacat ttttttcttt cgctagatgt gctatttcca aatgaatgat
     9961 attgggtttt gcttcaggat gataaatctt tttgacaaat tgatagaatt tatccggttc
    10021 attcaccatt gcttgatgac tcagcaagta ttccggctgc tccatcccgt ggtagattcc
    10081 tgacaaagat cgatagtctg gcactccaga aggtgtggat acccctgcac cggtcaaaaa
    10141 tgtaatttgc tgataagcta atatctcttt tgctgcttcg tctactgtta attgtttcat
    10201 gttttgtcac ctcactttcc attatagccc atactcgaac aaatttattg ttgaaaagtc
    10261 agaaaaatgg gaataaaaaa gtgttatggg catttgagca atttatcaca atgttcactg
    10321 acaaaagaat gaaaacgtgt tacaatactg ctgtataaaa gtgaggtgaa tcgatatgtt
    10381 accattcttt atttattggg gcgttattct ggcttgtatc gcctggttag cgttaagcgt
    10441 ttatttctct gttttttatc ttgtacgcaa agaaaatgga aatctttggg cttttgcttt
    10501 attcaacgtg attgccgcaa ttgttttagc cattacatta gccgtatatc gaacatgggg
    10561 ctggggaatc acccaatact caagtttgat ttatctaata ttagccattt atggcgtcgt
    10621 tgttcttttg caagcgatcc taggacgtga gccaaagaaa gcggcagctt aaatgatcga
    10681 gatggaactg gacacaggaa attttctgtt ctagttcttt ttttattgta ttacatacat
    10741 ttttgtgtat aattatacta tcgtttgcaa tggatagatg attcacattt tgattgtttt
    10801 ttcggtaaaa gtaaagattt ctatatttat cagtattttt tcaaaaacca gcagttatag
    10861 attgacaaag cctctcgagt atcgtaaact ttttttgtat aaataactga atacgagatg
    10921 cctcaatcaa tgaggtagag gtcgcgagaa tcattaaccc tgtggagtga agcaaacatg
    10981 gagaagcagg aatagggagg atcgccgaaa tgtgcacttt tttgcttgga gtgtatgttg
    11041 ggacgcaagt taagagcttg cggactgtct tagcagtgat gctaagttgc gctatgtttt
    11101 gtgagaggtt tatcacctta tggaatttta gcggcctttt tatatctata aatcggtcgc
    11161 tttttatttt ttcagataaa gctttaccgc atttcatcta gaccgctcgt gtatcatata
    11221 atcagaaagg gagaaaaaaa tgaaaaaaaa tatggtttca gttgccgtac tatttgcttt
    11281 tgtcttctca ctattcagtg tgggatctct ggcaaatgca gatgaaaaaa caccgagcga
    11341 tgagtttcgc gtaggtatgg aggctgggta tgcacccttc aactggtctc aacaaacaga
    11401 tgagaataat gccttgccaa ttcaaggcca aaatagttac gccggcggtt atgatgttca
    11461 aatcgctaaa aaagtagcag atggtttagg gaaaaaatta gtcatcgtcc aaacgaaatg
    11521 ggatggtctg gctcctgccc ttcaatcagg caagatcgac gcgatcatcg ccggaatgag
    11581 ccctactgct gaacgacgaa aagaaattga ttttaccgat ccttattacg aatcacaatt
    11641 agtagtagta gttcaaaaag atagcaaata tgcaaaagca aaaagtttag cagatctttc
    11701 tggagcaaag atcactgctc agttaaatac attccactat actgtgatcg atcagatacc
    11761 agatgtgcag aaacaacaag caatggataa tttctcagca atgagaacag ctttagcttc
    11821 tggaatgatc gatggttatg tcagcgaacg tccggaaggt gtaacggcaa ctagtgtgaa
    11881 caaagacttg gaaatgcttg aattcagcgg tgcagatggc ttccagacaa acccggaaga
    11941 tgttcagatt tctgttggta tgcgtaaagg cgacccggat atccaaaaag taaaccagat
    12001 tctttctggc atctctaacg atgaacgaac caaaattatg gaccaagcag tcaaagatca
    12061 gcctgctgca actgatagtg aagaaaaacc tggcatcatt tctgatttca ctacgatttg
    12121 gaaacaatac ggcaatatgt tcttacgagg tgctggactt accttgttca tcgcattgat
    12181 cgggacagtt gtcggtacta cacttggatt actgattggt gtcttccgtt cggtacctga
    12241 atcaggaaat gcactgactc gcttcttcca aaaaatcgga aatgcacttc tttctatcta
    12301 tgtcgaagta ttccgcggaa caccgatgat ggtacaagca atggtcatct tctacggtct
    12361 ggcactcgct tttggcattt cattgaaccg aacgattgcc gctttgttta ttgtctctct
    12421 caatactgga gcttacatga cagagatcgt tcgtgggggg atttttgcag tagatgaagg
    12481 gcagttcgaa gctgcgcaag ccattggtat gacccatggc caaacgatga gaaaagttgt
    12541 tattccacaa gttttacgga atatcttgcc agcaacagga aatgagtttg ttatcaatat
    12601 caaagataca gcagttttaa gtgtaatcgg cgtagctgat ttgttcttcc aaggaaactc
    12661 tgcttccggt gcgaatttcc aattcttcca aacatttacg atcgtcggga tcatctactt
    12721 agtcatgacc tttacgatca ccagaatcct gcgggcggta gaaaagaaaa tcgatggtcc
    12781 ttcagcttat accaagcttg aagaaatcga caattcaaat ttgcaggaat aaggaggaag
    12841 aatcatgaat gaaaaaatta ttgaaatcaa acatctaaga aaaagcttcg gtgaaaatga
    12901 agtattgaaa gatatcagcg taacagtcaa taaaggtgaa gttgtgacga tcatcggttc
    12961 ttccggttct ggtaaatcta ctttcttgcg ctgtatcaat ttactggaaa aaccaacagg
    13021 cggcgagatc atttataatg gagaaaatgt attagctcct aaatataatt tacctaaata
    13081 tcgaacgaat ctaggtatgg ttttccaatc cttcaacctg ttcaacaata tgaatgtctt
    13141 ggaaaactgt atgtctggtc aaacaactgt cctgaaacga gataaagaaa ctgccaaaaa
    13201 agtagcaatc gaaaatctgg aaaaagttgg tatggatcgt tatatcgaag ccaaaccagc
    13261 acaattatct ggtggacaga aacagcgtgt cgcaattgct cgggcattat caatgaatcc
    13321 agatgtattg ctattcgacg aacctacttc ggcgttagac cctgaaatgg ttggcgaagt
    13381 gcttaatacg atcaaagact tagcacatac tggcttgaca atgcttatcg tgacgcatga
    13441 gatggaattt gcaaaagatg tatctgaccg tgtcattttt atggataaag gtgtgattgc
    13501 agaagaaggt acaccagaag atatctttgt ccatccgaaa gaagaacgta caaaagaatt
    13561 cctttcacgt attttaaatg cttaggatca atagctgatt gggacaaaga gctgcggtgt
    13621 ttaccgcagt tcttttttat gaattactca taaaaggtaa atttctgaaa attttggaat
    13681 cttagttgtg tttttcagaa aactctctta atatggaagt aattaaactt ttttagttag
    13741 gatgatttaa aaatgaatga agagcaagaa ctgcagcgtg gactgaaaaa tagacatgtc
    13801 caactgattt ctattggcgg agcgattgga acggggttgt ttttaggttc cggaaagtct
    13861 atccagctag ctggcccttc gatattgctc gcctatctta ttactggatg catctgtttt
    13921 ttcattatgc gtgcgttagg cgaacttctg ttatcaaaca ccaataacca ttcttttctg
    13981 gattttgttg cagaatatct tggcaaaaaa gcggctttca tcactggctg gacgtattgg
    14041 ttctgctggg tatcgattgc aatggccgat ttaacagcca tcggaatgta tgtacgctac
    14101 tgggcgcctg gtgttcctca gtggcttcct gaactgattg cattaggtct tcttctttgc
    14161 ttgaatatgg tggctgtctc attgtttggt gagttagagt tttggtttgc attgatcaaa
    14221 gttgtagcga tcattgcctt tatcctcgta ggagcttata tgatcctcac ccactatacg
    14281 acagatattg gcacagccag tatcacgaat ctatggtctc atggcggatt ctttccaact
    14341 ggtgcaaaag gatttatctt agctttccaa atggtaacct ttgcttttgc tgggatagaa
    14401 ttagttggat tagtcgctgg agaaacagaa aatcctgaaa aagtgttgcc ggaagctatc
    14461 aataatattc ctatcaggat catcttattt tacttaggat ctctttttgt cattatggca
    14521 atctatccat ggaacagctt gaatccagat aacagtccat ttgtagaagt attttcggaa
    14581 attgggatca cgattgctgc ttccttgatc aatttagtcg tattaagtgc agcagcttct
    14641 gcctgcaaca gtgcaatata tagtacagga cgtatgcttc gttctttagc gcaagaaggc
    14701 agtgccccta aaaaattcaa aaagttaaca acacatcatg tgccagggaa tgctttgaca
    14761 ttttctacta tcgtcatttt catttctgtt atcttgaatt acgtgatgcc tagtgaagta
    14821 ttcacactag taagcagtat tgccactact tgtttcttgt ttatctggag tatgcttgtc
    14881 tacacgcaca tgaaatatcg taaaagtatc ctcggcaaaa aagctcattc atttaaaatg
    14941 ccgttgtacc catttagtaa ttatctggta tttctttaca tggcctttgt gtgtgtcgtc
    15001 ttgttcctag gaaaagatac gagaatcgct ttattactaa cacctgtgtg gttcttgctt
    15061 ctattactaa tctatcatat gaaatatgaa cgtaagaagt aagaaagtag ttgtgacaga
    15121 agtggtcagc ttcgagaaat aagaaggaat ttacgaaaat tgcttcttaa atttttgtga
    15181 aatttcggct tatttcttgg agataaaagc ttctgtccca gcctcttttc tatttccccg
    15241 gaaacaaccg ctgataatag gcatctgctt ttttatagga aaaaatccaa ataccagata
    15301 caaaaaaacg tccgaataac ccgatcaacg gtacaaaaaa gacaccggca atcaagcata
    15361 agttaatcat catggagata agacttcgac ggtcctttga caaaactctc acgacatggg
    15421 aatctggttt atttgctttc atgatttgcc tcatcaagat accaaaagaa aaactccaca
    15481 tcgtgtatac taagacataa gccatctcag gccaaaaagc ttcagggtta cgcccgaccc
    15541 aactagtaac agaaggacat aaagtcagcc aaaagagcaa aaagatattc gaccagacca
    15601 ctcggtaatt aattcgttct gccatctgaa atatacgatg atggttcacc cagatcaccg
    15661 caacaaatat aaagctgatg gtataagcta ataatgtatt tcggatgacc cataaagaat
    15721 gaaagctaga tgtattaggg atcttgatct ctaatacaat aactctcaaa acaatggcta
    15781 ccactccatc agtaaatgct tctaatctcg actttgtcac gatgattcct cctcagatga
    15841 ttcagcgctt ttattttatc acttacgtta ttaaataacc atcgttttat atagggaatt
    15901 ttctgaaaat tgttgcatat ttttcgaaat tttgttactt ttaaaaaaac cgtaaaggag
    15961 ttatgtatga caaaaaaatt atcatttaaa gactatcttt ttataggctc catgcttttc
    16021 ggtctatttt ttggtgctgg aaatcttatc tttcccgttc atttgggtca agaagcaggc
    16081 gctgctacct tttgggcgaa tctaggtttc ttggttaccg gtatcggtct tcccttctta
    16141 ggggtgattg ccattggtgt atcgaaaagc tcaggtgtat atgaattagc aaaacggata
    16201 aatcaaacat atgcgatggt ttttaccatc ttgctttact tagttatcgg accgtttttt
    16261 gctttacctc gccttgcaac cacatctttc gagattggtt tagcaccatt tatcgatcct
    16321 agcagacaga cgctatatct agcaattttc agtattgtat tttttgtgct ggcttggtgg
    16381 ttttcaagaa aaccgacaaa gatattagat tatgttggaa agttcctgaa tcctgcattt
    16441 ttggttttat taggtgcttt gcttctcctt gcttttttga atccactagg ttctgtaaat
    16501 caagcgccta ttcagccaaa ttatcaagag catgcttttt tcacagggtt tactcaagga
    16561 tataacacat tagatgcgct tgctgcttta gcatttggga tcatcattgt cacaacaatc
    16621 caaaacatgg gtgtaacaaa acctgctgaa atcgctaaag acacgatcaa atctggtgcg
    16681 atcagcatta tattgatggg aatcatttat actttgctcg cttatttagg tgctatgagc
    16741 ttaggtagct ttgctctaag tgaaaatggc gggatcacgc tggctcagat tgctgatcat
    16801 tatttaggta cttatggcag tatcttatta gcatttatcg ttatattagc ttgcttgaag
    16861 accggtattg gtttgattac agcttttgca gaaacattca cagatttgtt tccgcaaaga
    16921 aattacgcat tctttgttgc acttgctagt atccttcctt gtttagcggc aaacgtaggc
    16981 ttgacgaata tcattcaatt ttcgttgcct gttttgatgt tcatctatcc attagcaatg
    17041 acactgattt tactagtgtt agttggtcct ttgtttaagc agcgtacggc tgtttatcga
    17101 atgactactt attttacatt gattgcttcg atttttgatg ggttgaacgc ttgcccagaa
    17161 actatcaaac aaacacctat tgttcaaaat cttttatatg ttgcggaaag ctacttgccg
    17221 tttttcaaat tggggatggg gtggatcgta ccagcagtta tcggttttgt tatcggactg
    17281 atttggagtt tcgctaagaa agaagaagta gcagattgat aaaagaggtt gtgaaagaga
    17341 cgttcagcct tgagcaataa aaagtaattt tgaaaaatgg cttctcacat ttttgcataa
    17401 atttgactta ataccgagaa actgactttt gaacaccgtt tattgagagg tcgtgaaaaa
    17461 gccgcttagt ttcaagtatt aagaaatatt ttgtgaaaat ggcttctcac atttttgcat
    17521 aaatttgact taataccgag aaactggctt ttgaacaccg tttattgagc gtctataaac
    17581 tgacctgagt attagagaaa caaaacggca aagtagctcc atatactttg ccgttttgtt
    17641 ttgaactaat accgaagaga agctgatcaa acaacatttc ttttataata tagattttag
    17701 acatatatat gatataaaaa tagttttcgc aaaa
//
LOCUS       DAZKCN010000051        17715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000051 DAZKCN010000000
VERSION     DAZKCN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.052"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8K3_001653"
     CDS             4..816
                     /locus_tag="ACG8K3_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP9145529.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8K3_001654"
     CDS             813..1613
                     /locus_tag="ACG8K3_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9145530.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8K3_001655"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8K3_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP9145531.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8K3_001656"
     CDS             3110..4819
                     /locus_tag="ACG8K3_001656"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9145532.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="ACG8K3_001657"
     CDS             5031..9383
                     /locus_tag="ACG8K3_001657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP9145533.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="ACG8K3_001658"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="ACG8K3_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9145534.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="ACG8K3_001659"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="ACG8K3_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9145535.1"
                     /translation="MSKEMLNALDALETEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="ACG8K3_001660"
     CDS             11221..11514
                     /locus_tag="ACG8K3_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP9145536.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="ACG8K3_001661"
     CDS             11511..11822
                     /locus_tag="ACG8K3_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP9145537.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="ACG8K3_001662"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="ACG8K3_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9145538.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="ACG8K3_001663"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="ACG8K3_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9145539.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..15127)
                     /locus_tag="ACG8K3_001664"
                     /pseudo
     CDS             complement(14623..15127)
                     /locus_tag="ACG8K3_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15292..17655
                     /locus_tag="ACG8K3_001665"
     CDS             15292..17655
                     /locus_tag="ACG8K3_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFP9145540.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6038 a         3147 c         3901 g         4629 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccaaaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aaactgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaatccaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaaagat tatccttcgc tgaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgtatt
    17701 atttgtcaaa ttaag
//
LOCUS       DAZKCN010000052        17174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000052 DAZKCN010000000
VERSION     DAZKCN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.053"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8K3_001666"
     CDS             complement(543..2048)
                     /locus_tag="ACG8K3_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9145541.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8K3_001667"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8K3_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9145542.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8K3_001668"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8K3_001668"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9145543.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="ACG8K3_001669"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="ACG8K3_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9145544.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="ACG8K3_001670"
     CDS             complement(4752..5321)
                     /locus_tag="ACG8K3_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP9145545.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="ACG8K3_001671"
     CDS             5507..6409
                     /locus_tag="ACG8K3_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9145546.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="ACG8K3_001672"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="ACG8K3_001672"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9145547.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="ACG8K3_001673"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="ACG8K3_001673"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9145548.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="ACG8K3_001674"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="ACG8K3_001674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9145549.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12973..13158
                     /locus_tag="ACG8K3_001675"
     CDS             12973..13158
                     /locus_tag="ACG8K3_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP9145550.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13220..14416)
                     /locus_tag="ACG8K3_001676"
     CDS             complement(13220..14416)
                     /locus_tag="ACG8K3_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP9145551.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14586..16022)
                     /locus_tag="ACG8K3_001677"
     CDS             complement(14586..16022)
                     /locus_tag="ACG8K3_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP9145552.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            16315..17124
                     /locus_tag="ACG8K3_001678"
     CDS             16315..17124
                     /locus_tag="ACG8K3_001678"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9145553.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLNNQEDGLAVYLEKLLKL"
BASE COUNT         5046 a         3596 c         2976 g         5556 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgcccc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagccattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc atctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttcta tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt tatcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgta atctgtagga atgagttctt tcgcaagaac tcaaatttta
    12901 ttgagatttg gtctcgtttc tttacattca aagaaaatct ggtaaaatta agatagaaaa
    12961 gaggtgttct ttatgcgata tatcgttaca ttattctggg ctgtcgttct aggtcaagta
    13021 gtcggttata ttggtgctgc attaacaagc ggaacatatg attttacttt gacaacaatc
    13081 atttcattca ttgcgggtgt cattatcctt ttgattggtg cagttgcgcc aagaaaagaa
    13141 acctctgccc attcgtaaaa aaacagccaa gagcttgaga tatctcaagc tcttttttat
    13201 tttctgctcc tcaccaccat tataatctgc tgcgaagaac tttacgaaag ttgaataaag
    13261 aaagaaaaat atttgacaga acaaacaaac ttgtcacaat aaaaacagca ctgtaattat
    13321 aagcatgcgc gactgtcgat cctagcatgg gacctaaaac ttgtccaaag ttgctaaaca
    13381 tctgattgta gctatagatt cggctgactc cttcttgagg cgtaatctta ctgatcagtg
    13441 tattgacaga aggcatcaaa gcgccagttg aaaagcctaa aataaagcga agtataccta
    13501 attggagcgg tgtagtgacg aatgccatag gtaaataaca gcaaaatgat aaaatgagtc
    13561 ctccaattag aactttttga ttcccaatct tatcccctag ttttcctagt ttcggtgaag
    13621 aaaaaacagc ggaaacacca gctatcgaaa caatcaagcc gctgacaaaa aggatatttt
    13681 ctgtacttcc gctcaattca cgaatatata aggtcaaaat agggctgatc gtagtgattc
    13741 ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa aatagagaga tgatccattt
    13801 ttgaaaaaat ttctttggtg cttatcagat cttttttctc gatcggatgg aaatcttctt
    13861 tgactaagaa aatggtcaat accgttgtga tcatcaacaa agcccctgtg atcaagaata
    13921 cattttccat accaaaccac tgagccagtg caccgcccat tgaaggtccg atcagatttc
    13981 cagcaatcgc tccggtagac aatgttccta acgcccatcc gctcttttct ctaggtgcct
    14041 gtgaagcaat catcgctgtg gcatttggaa tatatccaga caaaattcca ttccaaaacc
    14101 gcatgatcaa aagccaatac gcgttcggta caaaagccaa agatcccatt gttaatgtca
    14161 tccctgctgc tgcacggatc atcatgagtt ttcgtccttt tcgatcagct agattccccc
    14221 agattggcgc aaccacggca gatgcaaaag cagtcacaga gattgctagt cccgaaaaca
    14281 attcgatttg atcttttggt gttcctagtt gctcaacata aactggtatg aatggcataa
    14341 ctaaactaat acttgcacca gtgaaaaaac agccaatcca cgcaatcact aaattttttc
    14401 tccaattaat ctccatctat catccctctt tcgtctcttt tatcatacct attttcgaat
    14461 aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc gcaaaggtca ttttatatgt
    14521 aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa tactgttcca atcccttctc
    14581 tttagttaaa ttgttgctag aatgatgaag ttgataatga ataaaatatc aacgatccac
    14641 aaagcaacag aaacttcatt gtattttcct ttgattactt tcacaattgt atagaaaata
    14701 aatcctgctg caatcccata ggaaatgcta tagcacaatc ccataaagat cgatgcaaag
    14761 aaggccggta aagcttcttc catatctagc catttgatgt ctgcaaaaga agccatcatc
    14821 attactccaa ctaaaatcaa tgctggcgca gttgcttgat ttggtacaat ggcgatcagt
    14881 ggagagaata aactacttaa tgcgaatagc actgccacta ctactgaagt caaaccagta
    14941 cgtccaccgg ccccgatccc agctgcagat tcgacataag ttgttgtatt cgatgtacca
    15001 aagatagccc ccacggaagt agcgatcgca tcggcaaata gtgctttatc catttttgtt
    15061 ttgaacccac gaccatcttc caacgcgatc tcgtcttctt ttgagaaaat gcctgtgcgg
    15121 cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat cagataaact gaatgcgatg
    15181 atcgtcatca atacttgagg aatcttggaa gaatcactga ataaagattg catgccttca
    15241 gaaccaaatg ctgcaccgaa agttgttcct aattctttaa atgaattacc taacgaattc
    15301 gtttgccaat caatggaact taaatcaaca acacccataa aaataccaag gactgtggtc
    15361 gctacgatac cgataagaat agccccacgg acatttttta caactaaaat ggctgtcagt
    15421 accaaaccaa tcacagccaa taagatagga gcgttgtcaa agttggctaa cgcagggacg
    15481 attccaccat ttatcgtaac atttgtcgct tttccgcctt caacgacaga gctcgaaata
    15541 gctgactggt ctgctgaaaa agataagaat cctgcatttt tgattccgac atacgcaacg
    15601 aaaataccga tcccgccacc aatcgcatgt tgaagacttt cgggaatcgc ccgaataatc
    15661 atttttcgaa tatttgtgac agtaataaaa atattgatta atccgcaaat aaacaccatt
    15721 gccaaggctt gttgccaaga ataaccaagc ccaaaaacaa ccgtaaaagt gaaaaatgcg
    15781 ttcaatccca taccaggtgc ctgagcatat ggtacatttg cgaacaatcc cattaccagc
    15841 gtaccaataa tcgatgcaat gattgtcgct agaaatactg cttggaaggg catacctgat
    15901 gctgacaaaa tcgtcggatt tacgaataag atataactca tggcaaagaa tgtcgttact
    15961 cccgccatta tttcagttga tacatttgta ttgtttttct ttaaaccgaa aaacttttcc
    16021 ataaaaagtt ccactccctg aatttttttc tgatggaaaa ctataataat aaaaaataag
    16081 taaaataggg caaatccatc gaacaatttc aaattatata gacgtttcat tcgcttttca
    16141 acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt tcgtgtttag cttatgttta
    16201 gctatttata tagccataac tgtagttttt gttaggaata gctgattgct cttgttaatt
    16261 acctaaaata tgctagacta aaacaaatgc tagttgagga aggaaggacc cttattgaat
    16321 aaaaaattaa ttgccatcga tctggacgga actaccctca atcaagattc gatgattaca
    16381 tctaaaacaa aagagactct gaaaaaagca atcaatgccg gtcatcatgt cagtattgct
    16441 acaggacgcc cattccgaat gagccatcag ttttaccaac aattagaatt aacaacaccg
    16501 atggttaatt ttaacggcgc attagtacac atccctaatc agcactggga tggtgaaaga
    16561 gaaacgttga tcaatcgtga aatcgctttt gaaatcttat cgcaaaaaaa gcagctaaat
    16621 ttagatttta tcgctgctga aaatcgtgat acttttttta ttgacagttt tgatttcttt
    16681 gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga atttgctttc tcctaaaaat
    16741 ttgacgacaa atcctacatc tttgcttgtc cgaacagaca aacgttttgc aggagctgta
    16801 tctgctgaat taacacgtca atttggcagt tatgtcgatg ttcggacatg gggtggacct
    16861 actgccatct tagagattgt ttcaaaagga atccaaaaag caaaaggcgt gcaagagatt
    16921 gctaactacc tgtctatcga tcaaaaagac gtcattgcct ttggtgacga gcataatgat
    16981 ttggaattgt tggattatgc aggttgggga gtagctatgg ctaacggtac tgatcaactc
    17041 aaaggcattg caaatgatgt cactcctttg aacaatcagg aagacggttt agctgtttat
    17101 ctggaaaaac tacttaaact ataatagaga attagattcc acaaaaaaga ggttgtgaca
    17161 atttttgtca caac
//
LOCUS       DAZKCN010000053        17152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000053 DAZKCN010000000
VERSION     DAZKCN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.054"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..700)
                     /locus_tag="ACG8K3_001679"
     CDS             complement(155..700)
                     /locus_tag="ACG8K3_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9145554.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(712..1404)
                     /locus_tag="ACG8K3_001680"
     CDS             complement(712..1404)
                     /locus_tag="ACG8K3_001680"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9145555.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1423..1707)
                     /gene="macP"
                     /locus_tag="ACG8K3_001681"
     CDS             complement(1423..1707)
                     /gene="macP"
                     /locus_tag="ACG8K3_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP9145556.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1720..2283)
                     /locus_tag="ACG8K3_001682"
     CDS             complement(1720..2283)
                     /locus_tag="ACG8K3_001682"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP9145557.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2511..3173
                     /locus_tag="ACG8K3_001683"
     CDS             2511..3173
                     /locus_tag="ACG8K3_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP9145558.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3297..4493
                     /locus_tag="ACG8K3_001684"
     CDS             3297..4493
                     /locus_tag="ACG8K3_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP9145559.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4594..5970)
                     /locus_tag="ACG8K3_001685"
     CDS             complement(4594..5970)
                     /locus_tag="ACG8K3_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9145560.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6007..6873)
                     /locus_tag="ACG8K3_001686"
     CDS             complement(6007..6873)
                     /locus_tag="ACG8K3_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9145561.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6962..7987)
                     /locus_tag="ACG8K3_001687"
     CDS             complement(6962..7987)
                     /locus_tag="ACG8K3_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9145562.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTVTLFFPIL
                     NFYNEKR"
     gene            complement(8005..8679)
                     /locus_tag="ACG8K3_001688"
     CDS             complement(8005..8679)
                     /locus_tag="ACG8K3_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9145563.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8863..9756)
                     /locus_tag="ACG8K3_001689"
     CDS             complement(8863..9756)
                     /locus_tag="ACG8K3_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP9145564.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9830..11527)
                     /locus_tag="ACG8K3_001690"
     CDS             complement(9830..11527)
                     /locus_tag="ACG8K3_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP9145565.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11520..12833)
                     /locus_tag="ACG8K3_001691"
     CDS             complement(11520..12833)
                     /locus_tag="ACG8K3_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP9145566.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12833..17102)
                     /locus_tag="ACG8K3_001692"
                     /pseudo
     CDS             complement(12833..17102)
                     /locus_tag="ACG8K3_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4836 a         3847 c         2969 g         5500 t
ORIGIN      
        1 aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataagt ttttttaaaa
       61 taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct tttattattt
      121 tgtgcttttt attttatcaa atcactttgt ttttttataa aatttctgcg cctagtgtgg
      181 actcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc acggcatctt
      241 tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc aacacacaga
      301 tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc aaacggatgt
      361 ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa acattttcct
      421 cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt ccacccgttc
      481 ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct tttggatcat
      541 gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt gtatgagcag
      601 tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca tcctctgcaa
      661 caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc cttatacgag
      721 atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt cgataaattc
      781 gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa
      841 tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt cgcaagctaa
      901 tgcttcaggg aaattcgtta agatttcttt gattttatct ggcgagtcga caaatgtatc
      961 acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg ctttaaccat
     1021 ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta attgtcctgg
     1081 gttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg atataacgat
     1141 gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga tcaccatgtt
     1201 cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt ttcctatacc
     1261 ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata aagcgcctgc
     1321 gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt cttcttccat
     1381 tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa atgcgattgc
     1441 taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca aaaaagagtt
     1501 ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt tattcttttt
     1561 gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag ctgtttctgc
     1621 ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct
     1681 tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt tggtcaatag
     1741 ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc
     1801 ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt catcttgtgc
     1861 taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga cttcatttgc
     1921 aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac gatagccagt
     1981 ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc ctggatcgat
     2041 ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag
     2101 aagcagacga tcttgttcgt caaaagcaat gatcccgaca ccgccaggat gaaataccag
     2161 ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa catcaataat
     2221 ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct cattgttcag
     2281 catatcttct cctgctttct ttgtaatcat aactgctttt ttttcatttt tcaatttttc
     2341 tcaaaaactc cttattagtt taccatagat aaaccttaga ttttctctaa atatagaaaa
     2401 tccttcttta gtccgatact ctttatagta gtccgtatgc taaaataagt atgtaatcga
     2461 ataacgaaaa gaattcaata ctcaacgata aatgacatgg agggtttatt atgataaaac
     2521 gaaattggcc gtggcttcta gcattggtct tgctatttct ctttgtccga aacaatagtt
     2581 tttcttttgt tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg
     2641 gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc actgactgac gaacttgaaa
     2701 ctcactatga aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga
     2761 atcaaacaaa aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta
     2821 aagcaatcga tctccgccat gatccagtca aagcagcgaa agctttattc aaagaattgg
     2881 tgaaagaacc aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc
     2941 tagttgattt gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga
     3001 cctatgaaaa actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac
     3061 aagattatca aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg
     3121 cgaaacagag cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt
     3181 gttcataaaa gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct
     3241 aaagtagaga cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg
     3301 atcataaacc gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat
     3361 tgaacaaccc gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg
     3421 atcagctcca agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc
     3481 aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca
     3541 gctacggatc ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg
     3601 tgcaagcaca agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc
     3661 aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg
     3721 ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa
     3781 ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga
     3841 tgttagaaca gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg
     3901 ctgctggaga attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa
     3961 gagcagaaga aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc
     4021 tagatcgtct ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc
     4081 aagcaccaca aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag
     4141 catctatcgc tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc
     4201 ttcgtcaaaa agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat
     4261 tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac
     4321 gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc
     4381 aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc
     4441 ttggacacat ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct
     4501 ttcgcttgtt actaaaacaa acgcccaaca acagtttatt acaactgttg atgggcgttt
     4561 ttcttatctt tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc
     4621 atagccatag ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca
     4681 tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc aagatggaga cgattggcaa
     4741 tagcggaacg aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa
     4801 cttcagtaag cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc
     4861 cgctaataca ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata
     4921 agtcgctttt ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa
     4981 gccatccttg ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agatcgttac
     5041 cgtcagtagc gttaaaattg cccctacagc gatcaccgcg cccactgttc catgtcctac
     5101 atacctcatt gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc
     5161 tgtcaagatc agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat
     5221 tcctcttggc acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc
     5281 aaatcccaag aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa
     5341 aggcatgaat ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa
     5401 cgcgatgata ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc
     5461 ttcaagcgac acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta
     5521 tgtaccattt tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact
     5581 tttcaaaaaa ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa
     5641 aaattccccg atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata
     5701 cagataggca tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag
     5761 tccagacaaa atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc
     5821 attttgattg gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat
     5881 tccaagcatg atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag
     5941 attgatctct ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa
     6001 cagcgattaa actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca
     6061 accaacaata acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc
     6121 acctaaagta gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag
     6181 tgatttgact gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat
     6241 gtttttccac acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa
     6301 gatcattccg atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac
     6361 gatgatggta tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc
     6421 aaaatcaagc attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa
     6481 atacgcaccg cttacaagaa gagctaaagc aataataccg tatacataat ctttcgtttg
     6541 tgtccattca tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt
     6601 agacaatggt agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg
     6661 tccaacactc caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac
     6721 aggactaaca acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag
     6781 tgtttgttga ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc
     6841 caacataggg actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat
     6901 aagaaactta tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt
     6961 tctaacgttt ttcattataa aaatttaaga ttgggaaaaa taaagtcact gttgtccctt
     7021 ttccttcagt tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta
     7081 aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc
     7141 ccttatcaaa gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg
     7201 aaaggctgac tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg
     7261 tatatttgat ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg
     7321 tcaaaacagt atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat
     7381 ttgcctgact gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt
     7441 agtcccgcga gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga
     7501 tttttgataa ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg
     7561 aacgaactag caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa
     7621 tataatcttt ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac
     7681 ggatcagctg attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca
     7741 aataattttg caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct
     7801 tgctcaagta accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat
     7861 aagcaattgt gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca
     7921 atgcaataaa aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa
     7981 aattcatctt aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc
     8041 gatatatcct tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt
     8101 caatgtattg tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg
     8161 acttaagatc tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct
     8221 caaatcaatg atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc
     8281 attgtggctc atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc
     8341 attgattttt gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc
     8401 catgttcata gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg
     8461 tactttcgat acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt
     8521 aatatctagt aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa
     8581 atcggtcgtt ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg
     8641 aatgatctct tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct
     8701 tcttgctact acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt
     8761 cattttagca aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc
     8821 aagcagctta aatttattat gttattctta ataattggtc tattatctgt tatttttctt
     8881 tcgttgtttt tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag
     8941 caatatacct agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat
     9001 ctttgaagag acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt
     9061 gttcacggtg taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc
     9121 taatgtattt gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat
     9181 ccgaccatac acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa
     9241 gagttttttc tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc
     9301 agttggaaaa gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt
     9361 ttcatcaaat aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg
     9421 ttgttcgtaa tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc
     9481 tttgacttgt cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc
     9541 atgcactgct aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata
     9601 attctcgatc cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt
     9661 aaaaactgca gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat
     9721 ttgggtttga ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa
     9781 aactgcagca acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag
     9841 acttcatttt agatttacgg taccagctgt acgctccaat catcaaactt attccgatta
     9901 gtaagaatgc caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg
     9961 gtactctttc tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc
    10021 cataagagat gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg
    10081 gcgccatggt ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc
    10141 catttccatc tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg
    10201 ctcctgattc ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac
    10261 ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat
    10321 atttttcaaa ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat
    10381 aatcaaattc atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg
    10441 cttctagttc tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat
    10501 ctgctaatct acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca
    10561 cgacaaaccc gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct
    10621 cgccggctcg cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg
    10681 tgattttgta atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag
    10741 gaataggtgc tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa
    10801 tcgtttcttg ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta
    10861 cattcatctg tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat
    10921 tgtatgtgtc tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca
    10981 ctggcatcat aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt
    11041 ctaagaaaag atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag
    11101 agaataccgc ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt
    11161 ttaaaaacca actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa
    11221 gaccgtcttc tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg
    11281 ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa
    11341 ccaatccgct attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa
    11401 tcgtcacttc acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga
    11461 aaatccctat tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt
    11521 tattcatttt tttctccccg ttcttttttt attaataaaa cagcacctaa caataaaaga
    11581 attcctagta aagaaatcaa cgttcttgct tcattcgttt gcgggaatac ggaccatttt
    11641 cctgattgac ctggattgct tggaccactc ggctctttcg gtttgtccgt tgtttctttt
    11701 acggtctcgt tataaacacg tggtgttgcg gattcatagg cagagactgg aactttcccg
    11761 gattctaatt cagccatctc ctgtccgtta actactacat attgtggttg gccttttgca
    11821 tccacccaaa attcagggat gatgacctcg attctcttac ttgcttcatc catctcgtaa
    11881 ccttccacac cttttaattc ttcaaaataa taggtgccag aaggcaagaa acgttgtcct
    11941 gtcgtaacaa ggccctcttt atccgaaatg aatttgctta catttttgtc attcaaagga
    12001 tcattgtctg ctggtttgat ccattgattt ttcaagtcat tagcaggagc catatctaaa
    12061 taatatttcc ccccttctat catctgatat agagcaaaaa cagcaccttc taatggtttt
    12121 cctttttcag ttgttcctct ttgacgtcca tacttgaaga agtatggatc tcttagataa
    12181 ccgacatttt taggatagat atgaatctct tgcagttcct cggttgattc tgtcgggtga
    12241 taaattggca aaatagcagc aattgggata gctgtctgat cgatatcaac atccaatcca
    12301 acttctggat caatcgattc ttcgaaaatc aaataaactg catcccgatc ttggtttttc
    12361 ttaggtgctt tgatacgtgc aatcccgtct cctaatccgc caaccgtatc tttcccagtt
    12421 gttaccgaag tccctatttg gacaagcctt tcttctgtga ttagctgacg gatattgttt
    12481 ctgttcgttt cagcgatttg ctggacgaat tctttaggag tcatgttttc agcagctttt
    12541 ttttcatgat aataatcggt catgtcgtaa atggcaaatg tcgctccatt tagtggcacc
    12601 gtctgtgaaa tgatatctgc tgtttcccct gcttcctcag agatagccaa gccatcattt
    12661 tcatggtatt caaattgatc gttataatcg atatctcgaa tcatccgttt atgcaacacg
    12721 aattccacga catcttcccc tgattcttct gccgacatcg catgtggaag aaggactgct
    12781 gcaagcggta gtaaaacaag taattgaagc atcaaacaca acagcttttt cattaccttg
    12841 ctccctcctt tttagattgt ctgacaaaca aaccataggc tgctcctgca agcgccatga
    12901 agaatgcagc aagcaggata tagaataatc gtccggatcc tcctgtagca ggcaacggat
    12961 tttcaggatc gttcccgaca ctaagctgta atctgattcg atttgtctcc aaaagttcat
    13021 attctaactc ttgctgtaga tcaggcgtct ctgcttcttc gtttggataa tacgaaatct
    13081 caacttttag tgtacccgct tcttttcctg gaactacccg atcattttct atgtcaggtt
    13141 cttctcgata actaatttct aaaataaagt aacctggaag ttttctatag ctgtcaggtg
    13201 acttcacttc cttgatccga tagacccctt ccaacaagtc tgaagcattg aatagggaag
    13261 aatcgtttgg atctggtgcc aagcctccct cgatgatttc atactctcct gcactatttt
    13321 gtttctcaat tgaaaattct gcgccttcaa gacgtcggct attcatactg tcaattttca
    13381 aaagttccaa atcgattggc gctagttcat cttctaaggt tacttgtagt acaccatcga
    13441 tgatttcata gccttccggc aagggagcag tttcgtcttc gatgaccgta tcttcaatga
    13501 taaacttacc ttcttcattc agtgcaaact ggaacacttg atcagatagc cgatacccat
    13561 ttggtgcgac agattccgag acctcatatt caccaataga cagatgcaga ggttggtcgt
    13621 aatttaaatc gacatacttt tctactccta cggcacgaga atagaagtat ccgatcccag
    13681 gcaaacgttt cgattcttct ggttctaacg gattaccagt gtatgctcga tacagcggga
    13741 aggaagaatc ccccactttc ctgatatcga aacctgcacc atctaatgta tggccttcat
    13801 ggtctaattt tctaactcgg atctcccgat aatcaaacgt attgtaagca gtgaatgcta
    13861 cccataaacg atcctcttca tctgttgtat acgtgagata ttgccaattt tcttcacctg
    13921 tttgcttgag ttggatagtc cctatagcat tatcgtaaag tcgcaagatc catgtgtcat
    13981 tttcatcagg ttctttgaaa ccttctgggt tctggctctc aatgattttg tattctgcat
    14041 agttggtagt ttcttcacta tccttataaa caaatgaatc tggctctccg attggatgaa
    14101 taactttctc accatccacg attacatatt tcccgtcttc atctactgaa tagaacgtga
    14161 gtctcccttt ctcttcctct acttcgttca caccagaaag ccctttcgct actggatatc
    14221 cgatttccag tccgttacca tctgtttcat aaagttcaaa ctctgcgcca ttgattggca
    14281 tattactgcc ttcaattagt ttattgatat tcatgtggat tggcttgaat tcatttcgca
    14341 tttccagttt gacatacaac tcatcatact ctgtgccttc tatgttaggc tcttgtacaa
    14401 cagaaagctt gtgatattct ccttcataat aggaattcgg atctttacct tccggtccgc
    14461 cgactctcga accagatgta tattcatcta caagctcgta gtgacctaac atctcatctt
    14521 cgttggctgc atattttttg atttggaatt gatacgtctc atctactaac tgataacgtg
    14581 gtggtccagc gatctctctg atccaaaata tgtcgctgac tgctccagtt tcatctccta
    14641 aaatcggcat cttcgtttca aagatcgcta ttccgtccgt ggtagtttta cttgtttcga
    14701 ttggttcggc ttccccactc tctggatttt ggtcatataa tgcaaattcg actccagcca
    14761 acggctcgcc attttgaccc ttcttgctga cttggaaatt acggccttta tgttcgtaac
    14821 gaatggaaga agatgggaaa tacatccgtc cagtagtgat atcattctca taatcctcca
    14881 ggttctgaat ataggccata ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat
    14941 caagttttac ttggtacttg atcctgacca tttccccttc acctaaagaa atcggagaaa
    15001 atttataagc ttggttttcc cgtccgacat acgttgtcgg agtcgccact acttctggtg
    15061 gggcttgtcc atgacgttct tcatcccata tttctggctc ttggtacttc atgccgtttt
    15121 ctcctggatc tagatggtaa aactgaatat ctgccgatct gccagctcca aatggtgaga
    15181 cattaaaacc agtttcatca taaagtgtgg cttgctggat cgttctcttg aaggtagaag
    15241 agatatcctc caaggctgta acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg
    15301 ttacgccatc ttcttctaaa tcagcaatat ttttcaatac atttcgtcca atagcatcta
    15361 gtcgttgtct cccgctaacg tttccatcga ttcctaaacc aatggagaag atacttggcg
    15421 ggttctcttg atacttgttt ttcagccaat gatgataagc aactgtgttg atcgcagatg
    15481 acttttcttg cgaagtagga ctgccattcg tattgttatt agaagaacca tcaccaaccc
    15541 agtgagtcgc tttattatct ccttctttta cctctccata acgatagtgt acatttactt
    15601 catcttctgg acggttaaag tcagatgcat aggtaactgg atgagtaaac cctgcattag
    15661 aagtattccc agaaaactcc tcaaaatttc tgaacaaaac gtcattatct tcagcaatct
    15721 tatcttccat cacagtccag ttgctccaag ctccattagt gctcgtacga taacttgact
    15781 gaatcggtgc ataactgaag gtcggtgtcc catctcccac aacgattagg attttttctg
    15841 gatttctatt ttctccgcca ttgtctttat acaatgtttc atagccattt ttaagcccta
    15901 gtgtaagcgg agttcctccg attggtgtac gatttaatgc tgtattctca cttcccgtca
    15961 aacgattgat atcatttgtc aatggaaaga tgtccgaact gatttgctca tggttattga
    16021 tagctgtact attatggtaa aaattcacca taccgattcg aatatccaga ctcgggtcag
    16081 agagcagatc tttactcatt tcgtttactg cctcaatcaa cgcagcattt ttagtttgac
    16141 tattggtccc agcggttaat tcactcatac tcgctgactt atctagaaca aaaacgatat
    16201 cgattggact gatttccttt ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg
    16261 ttgttgcatc ttcaggatgt agatcagatg ctgtgctgct tggcagaaca ggttctaccc
    16321 attttttgat gtacgccccg ttataatcca agtaactgtt ttcaaaattc cgttcatttc
    16381 cccaaagctg aacggtttga gctggaacgt ttctatcttc ttcgcctgca gttgatccat
    16441 tattagtagc atagtcgtaa tttcgccagt tcgctccccc gtttgtataa tcactatcat
    16501 cgatcaagtt ctctccgtca tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt
    16561 gtgtccaatt tcctaacact tttgtatagt cattcgttgt aaacttcatc tattctttca
    16621 tcaaccacca cttgaagagg tagttaccct tcgttcacat cggggatctt ttctgtttca
    16681 aaaatcaatg tgccggtttc tgcttctttt gaataaactt ttccgacata ccattcttga
    16741 ccatcgatcg tctcgacttt taggtcttct ccatcttcat cataggagga tagacctgtt
    16801 ccacgaacgt tttttacggt accaatcccg gcggctgccg catcgattgc taaacgtatt
    16861 ctaccttcat tggcactttc ctttttttca aaagccagat gccattcaag ggtttcttct
    16921 ttttcaatat acttcccttc aatttttgca tagtcctcat ctaagaatac ctcatcctcc
    16981 tttgtttctg ttgactcagc agctacatcc agggtcgtca tatgatagac tgactggaaa
    17041 tatggtccaa atagcaaact gacagttact aactgcaaaa gcttcaattt taaccctttc
    17101 acatccttat actcctttca aaaatatttg tcttctttca caacaatcaa ca
//
LOCUS       DAZKCN010000054        17105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000054 DAZKCN010000000
VERSION     DAZKCN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.055"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8K3_001693"
     CDS             <1..1152
                     /locus_tag="ACG8K3_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFP9145567.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8K3_001694"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8K3_001694"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9145568.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8K3_001695"
     CDS             2728..3003
                     /locus_tag="ACG8K3_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP9145569.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8K3_001696"
     CDS             3009..3776
                     /locus_tag="ACG8K3_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP9145570.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8K3_001697"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8K3_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP9145571.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8K3_001698"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8K3_001698"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP9145572.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8K3_001699"
     CDS             7920..8222
                     /locus_tag="ACG8K3_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP9145573.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8K3_001700"
     CDS             8225..9388
                     /locus_tag="ACG8K3_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9145574.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8K3_001701"
     CDS             9414..12842
                     /locus_tag="ACG8K3_001701"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP9145575.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8K3_001702"
     CDS             12863..13987
                     /locus_tag="ACG8K3_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP9145576.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8K3_001703"
     CDS             13968..14414
                     /locus_tag="ACG8K3_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP9145577.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8K3_001704"
     CDS             14398..14736
                     /locus_tag="ACG8K3_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP9145578.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8K3_001705"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8K3_001705"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP9145579.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8K3_001706"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8K3_001706"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9145580.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8K3_001707"
     CDS             15893..16936
                     /locus_tag="ACG8K3_001707"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP9145581.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5800 a         2958 c         3769 g         4578 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagt
//
LOCUS       DAZKCN010000055        16929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000055 DAZKCN010000000
VERSION     DAZKCN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.056"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(271..557)
                     /locus_tag="ACG8K3_001708"
                     /pseudo
     CDS             complement(271..557)
                     /locus_tag="ACG8K3_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(734..1912)
                     /locus_tag="ACG8K3_001709"
                     /pseudo
     CDS             complement(734..1912)
                     /locus_tag="ACG8K3_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1942..2352
                     /locus_tag="ACG8K3_001710"
     CDS             1942..2352
                     /locus_tag="ACG8K3_001710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145582.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2433..3053
                     /locus_tag="ACG8K3_001711"
     CDS             2433..3053
                     /locus_tag="ACG8K3_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145583.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3754..4038
                     /locus_tag="ACG8K3_001712"
     CDS             3754..4038
                     /locus_tag="ACG8K3_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145584.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4041..4958
                     /locus_tag="ACG8K3_001713"
     CDS             4041..4958
                     /locus_tag="ACG8K3_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9145585.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4951..5733
                     /locus_tag="ACG8K3_001714"
     CDS             4951..5733
                     /locus_tag="ACG8K3_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9145586.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5734..6414
                     /locus_tag="ACG8K3_001715"
     CDS             5734..6414
                     /locus_tag="ACG8K3_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9145587.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6407..7303
                     /locus_tag="ACG8K3_001716"
     CDS             6407..7303
                     /locus_tag="ACG8K3_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9145588.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7498..9147
                     /locus_tag="ACG8K3_001717"
     CDS             7498..9147
                     /locus_tag="ACG8K3_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9145589.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9741..10676
                     /locus_tag="ACG8K3_001718"
     CDS             9741..10676
                     /locus_tag="ACG8K3_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145590.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10669..11439
                     /locus_tag="ACG8K3_001719"
     CDS             10669..11439
                     /locus_tag="ACG8K3_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145591.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11855..12838
                     /locus_tag="ACG8K3_001720"
     CDS             11855..12838
                     /locus_tag="ACG8K3_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP9145592.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            13285..14322
                     /locus_tag="ACG8K3_001721"
     CDS             13285..14322
                     /locus_tag="ACG8K3_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9145593.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(14411..14734)
                     /locus_tag="ACG8K3_001722"
     CDS             complement(14411..14734)
                     /locus_tag="ACG8K3_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP9145594.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14721..15428)
                     /locus_tag="ACG8K3_001723"
     CDS             complement(14721..15428)
                     /locus_tag="ACG8K3_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP9145595.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            15514..15855
                     /locus_tag="ACG8K3_001724"
     CDS             15514..15855
                     /locus_tag="ACG8K3_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9145596.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(16026..16874)
                     /locus_tag="ACG8K3_001725"
     CDS             complement(16026..16874)
                     /locus_tag="ACG8K3_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9145597.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5382 a         2896 c         3537 g         5114 t
ORIGIN      
        1 taatgaaaaa cgagaaggaa gatgacttta aagtagttta gtatgaaggt cgattgataa
       61 aacgatgtgg attaatgcta aataatgagt aatttttcaa taatacaaaa actgtgtaca
      121 gtattagaag tagagaaacg ctagcgtcga aaaaaagata tttttcgtga aaagtaatag
      181 gggctaaaaa gaaagtatac aatttattta tgtttctaag catataaaaa agagcgttga
      241 aaggaatatt tccccttcaa cgcttctttt ttagaagatt ttaatgacca tctcaacttc
      301 attttcaggt ttaggtaatt catggaagct tgtgtttatc gtgcttaatt gatcaactaa
      361 ccatgtttta ctttttgact cgtagacaaa tctaaaatta atatcattgt tctcgtgttg
      421 aagaggtttc tccaaataat ctggtgaata ttcatagtca acgtaacctg ctaatacaaa
      481 agaaagcagt ccattattta gtgtcagatt taacggatgg tcgtaagctt ttattttttt
      541 aatgtcatat tgtacatctt atgaagactt aatgactact tgtagtcaat agatttaaag
      601 tatttatttt ctcatttatg tatcaagaat aaatactgat ataataaaaa ttatatcaat
      661 gttttgatga agtaatttgg agggagcgtc aataattttg tgtaaataac tcgtccttct
      721 gcaaaataat gggttactca gtaaacattg aagctaatgt atccgtcact tgttggaagc
      781 ctttatggct tctatttaag aatttttgat tgtatgtatc aaagatggat actaggaaac
      841 gctctagtga ttcttcattt tgaaactgct cttttctgcg gctgtatttc ttgatttgtt
      901 tcttgaaaga ttcgattaaa ttagttgagt aaatgcttcg gcggatacca ggcggaaact
      961 cataaaaagt caataaatct tgatttttta tcagtgactg cgtcacttta ggataagttt
     1021 tttgctactt ctcaatcata ctccctaaaa aggtatttgc ctcttccttt gagctagctt
     1081 gataaacagc cttaaaatca tcacagattt cttttcgatc tttgacacgt actttatgag
     1141 caatattacg ggaaacgtga acacagcaat gttggtattt agctttagga taaatctggt
     1201 gaatcgtatc tttcatgcct tttaagccat cggtaataaa cagtaagaca tcatgaaccc
     1261 ctctggagta aatgtcctgt aacaactcat tccaaacgta ggtcgattca gtcggagcaa
     1321 tcgcataact tagtacttct ttagtgccgt cttctcgtat accaatggca atatagattg
     1381 cttctttgga gacggtttgc cgttttaaag gaatataagt agcatccatg aaaatagcga
     1441 catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc agcactttag
     1501 tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca atcaagtctg
     1561 cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc tctaaggtat
     1621 cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccgttg cggtcgcgcg
     1681 gaatctggag atgaagttcc ccgtactcgg tcttgaccgt acggtcatag gagccgttac
     1741 gagaattccc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta
     1801 actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat aattcattca
     1861 aatcgccttt gttcgctaga gtctttagaa tttctgtagt aaaatcgttc atatggaagt
     1921 cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct
     1981 atttacacaa aatattttac actctcgtca aatataaaaa ctagtacgaa attaatttgt
     2041 aaattattta agcttgataa aataagcaat atcgatgatg taaagtccaa gatattaaca
     2101 agaaaagtaa gcgaaccagt aaagctaacg gcacaagata aaaaaaatgg aattgttctt
     2161 tatggtgatg atgcaaagta taactataat tgggatacct cctcagaagc tgaggtatta
     2221 agtattccta ctaccgatac ttcaggggaa aaagtcacct ttaaaataaa ttttataacg
     2281 gcaatttcta agttggagta tgggaagcaa aaaaagagag ataacaatga tcaatatcta
     2341 cctaaaaaat aacgaattaa tgatgcatat gttaaagttg tttttttgaa ttaaacaact
     2401 caatttattc agttatatgt tcatcgcagc aagtgaacat taatcgagtt ataaaattaa
     2461 taggagctaa aaatataaat aaagttcgat cagaatttat ttttccagaa gattttttta
     2521 actggttttt ttatcaattc agtgaatgca agcgactctt aggtgagaat tattacttaa
     2581 aaggaatcgg cggatttatg gggaatgtgg ctgatgaaca aaatattata aaaggaatat
     2641 ctgaacaaac atcagagctt acagtaacta aagcatttat aagtaatggt gaaagtttga
     2701 aaaatgtaac tgcgcgaatt agaaaagaaa atgaagtaga cattgttttt ggaattgacc
     2761 atgaatgtaa ttcagtgata tatgtaacac aatcagcaaa tttaattctt tttgagaatg
     2821 gttgcaagga cgattttatg gtggtctatt tgtacgatat actaattcca aaacttcgtt
     2881 ccttatatat gtcttctgaa agtatttttg tgagtgaaca aaagggatct ttctcgaaaa
     2941 aaataggtat tgaagtaatt gaatcgatta tgtgtcataa tcatattacg ctagaagaat
     3001 tagaaatttt gatagggcaa aaaaataata acacgaaaat acaagtttct tagaaatcat
     3061 tttttattga gatatctaga taaatgattt attcttcaat caaccaactc gccaagctga
     3121 ggtaatgttt tgaggtaatg ataacataca actttttata ccctagtaaa actctataaa
     3181 caaagactta ttaatttcgc atgaaataca ttcctgtaaa atgcaataaa atagagtttt
     3241 gtttaaccag gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga
     3301 ttatcgttcg agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt
     3361 taccttaatc ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt
     3421 ttagctatgt taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg
     3481 ggaaaaagat aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa
     3541 gtggtcagct tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat
     3601 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg
     3661 tgaaaagtct tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg
     3721 tattattttt cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc
     3781 tggcttcagc gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg
     3841 attaccatgg tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag
     3901 acaactcaac cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta
     3961 cactttcttt atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc
     4021 aataagaaag aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta
     4081 taaccaaaaa atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg
     4141 gaatgatcta tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta
     4201 ttatgggttt gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc
     4261 aggcagcaag acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa
     4321 ctgcaagaga taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg
     4381 aagacctgct gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct
     4441 ctttaggtat gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt
     4501 tgattttgga tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa
     4561 tcatccaacg tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag
     4621 atgaattgtc acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag
     4681 aattatcgaa agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg
     4741 cgacggaaca agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat
     4801 ctagccgtgt tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat
     4861 tggtagaggc aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt
     4921 atttcttaca attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata
     4981 aaagtatgga agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt
     5041 ttttattctt ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca
     5101 acggatacag tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg
     5161 attcttctgg ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt
     5221 acgattcgca atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt
     5281 ctcgttacgc ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt
     5341 gcttacacac ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat
     5401 gttctaaaag tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg
     5461 gctttgacat tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc
     5521 atgggaacgg ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg
     5581 gagtggttta cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg
     5641 caagcaccga aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga
     5701 atgtttattt tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt
     5761 atcgaagacg atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa
     5821 cttcttgatg cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat
     5881 ttgatccttc tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc
     5941 cgacagacat cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa
     6001 gtttccttgt tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa
     6061 ctgttgttac gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg
     6121 tcgcaacgca tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg
     6181 aatggtgtgg ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa
     6241 catccgaaaa aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat
     6301 tttgatagtg agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag
     6361 gcacatgctg tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct
     6421 gttgcttctc gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa
     6481 gagattaact aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc
     6541 agtcgaattt cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga
     6601 tgaaaatgaa caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat
     6661 cgtcggcctt tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt
     6721 actggaacag gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc
     6781 tgttgcaaga ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa
     6841 gcaaaaagaa atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt
     6901 ctctttttat gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat
     6961 aaatacaact gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca
     7021 gaatttattg cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt
     7081 cattcgatta acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt
     7141 cttccagcgt ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg
     7201 gctttacctg tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga
     7261 cgggggcatc ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct
     7321 gaagtgaatg acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt
     7381 tttattttgg tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt
     7441 ttcaaatagc gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg
     7501 ggccaaagag aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta
     7561 gacatttttt cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct
     7621 tatcaaccga gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa
     7681 ttccgttcat gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa
     7741 gtatttgaag ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt
     7801 ttacgtagct ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag
     7861 gaaaatcctc aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat
     7921 cataattttg caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt
     7981 ttatttattt ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa
     8041 gtactaggaa tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg
     8101 aagaaacaaa cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca
     8161 gccgccaatc agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact
     8221 aaaccattaa gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa
     8281 gcagaattga tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat
     8341 ttcgttctca ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg
     8401 ggagaattgg aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc
     8461 cgaccagagt ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa
     8521 gaagcggtag ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca
     8581 tcaggaaaat ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc
     8641 caaacagcga ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca
     8701 atcgaagatg atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa
     8761 cgtttgctgg accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta
     8821 aaaggaacaa atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg
     8881 gagtatccat ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag
     8941 aattattgtg agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt
     9001 gatttcaaat taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt
     9061 ttgaattatc ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa
     9121 cagcgtcagt ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa
     9181 aaagacgatt atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac
     9241 gagtctgctg tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg
     9301 ctgtcttcct tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt
     9361 ccatggcaaa gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc
     9421 attttattta tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag
     9481 cttaacatga ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta
     9541 gtagttgctg tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc
     9601 tatcgaagat gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg
     9661 tgtggaagcc aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc
     9721 tataaggaga taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct
     9781 atggaagtgg ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct
     9841 atgcgctatt agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt
     9901 tagtcaataa aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag
     9961 ttcgagcaaa gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa
    10021 cggtccagca aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga
    10081 tgaaacgcag cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg
    10141 cacgtatgct ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg
    10201 aaccacgttt gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg
    10261 agatgtggga gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc
    10321 attatctaga agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac
    10381 tgatcgagaa tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct
    10441 ttgatttagc gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag
    10501 atgagcagac aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc
    10561 agcttacaag acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag
    10621 aactgttctt gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt
    10681 tattttactg cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg
    10741 gtccaaacac tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa
    10801 atgatcggtg ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct
    10861 ggtttgatta tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc
    10921 tctcaaaaat ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg
    10981 attatttggg gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta
    11041 acgatcattt ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt
    11101 acattattga tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct
    11161 cagtcttatg atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta
    11221 ggtggcgttt tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc
    11281 aatccgatcg tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg
    11341 ccagtagcaa catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact
    11401 tggtatttga tcaataaagg aagaggattg agaagttaga aaagaggttg tgaaatcagc
    11461 gttttgacct gagtattaga gaaacaaacg ggaaaatagt tcctcatatt ttgttgtttt
    11521 ttgagcgaat accgagggtc agctgattga acaccgttta gtggagaggt tgtgaaaaag
    11581 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
    11641 ggttggactt attaccgcaa atgcaagctt ttgaacacca tttattccgg aaaatggact
    11701 gtgataagat ttttgtcaca gtcttttttt tgctcaatca gaaacatctt ttaagctgtt
    11761 cactttgctt ctttttttgt ttcctagtat aatgacaatg tttttgagaa agattgcgac
    11821 ttcactgctt gatagaatgg agagtaacaa ataaatgact actaattttt ggaaagagct
    11881 gccaaagcct ttttttgttt tagcaccaat ggaagatgta acagatgttg tatttcgcca
    11941 tgtcgtaaag catgcagcag caccggatgt cttctttact gagtttacaa actcagacag
    12001 tttctgtcat ccagatggaa aagacagtgt acgaggaaga ttaacattta cagaagatga
    12061 acagccgatg gttgcccata tttggggaga caagccagaa ttttttagag aaatgagcat
    12121 tgctatggca gaaatgggct tccaaggaat cgatatcaac atgggctgcc cagtccctaa
    12181 tgtagcagaa cgtggaaaag gcagtggctt gatccttcgt ccagaagtag cggcacaatt
    12241 gattgaagca gctaaagctg gcggtttgcc tgtcagcgtc aagacgcgta tcggctatac
    12301 ggaaatgtct gagatggaag aatggatcag tcacctactt catcaagata tagccaatct
    12361 ttctgtccat ttgcgtacgc gtaaagagat gagtaaagta gatgcacatt gggaagtcat
    12421 tcctaaaatc attgctttac gtgaccaaat cgctccacaa acactaatca cgatcaatgg
    12481 agatatcccc gatcgccaga caggagaaga attggccgag aaatatggcg ttgatggcat
    12541 catgattggc cgagggattt tcaagaatcc ttatgcattt gaaaaagacc cgagggaaca
    12601 tacagaaaaa gaactgatcg gactacttcg tctgcaacta gatctgcaag atcattattc
    12661 agaaatcatc ccgcgttcca tcacaggact acatcgtttc tttaaaatct atatcaaagg
    12721 atttccagga gcaaatgact tgcgagttca gttgatgaat acgaaatcta ctgatgaagt
    12781 acgagagatc ttagagacct ttgaaaaaga acatccaacg cttttcagtg aacaatgatt
    12841 tcttgcttga acaccgtgaa ttagaggtcg tgaaaaagct gtcctgtatc aagtaataag
    12901 aacagccaaa caaaaatagc tcttcatatt ttgtcgtttt ttgagctaat accgagggtc
    12961 agttgattga acaccgttta tatcagaggt tgtgagcctg ccgtttaact ctgagtcaca
    13021 aggagcaatt tcaaaaaaca gcttctcatg tttgatgaat attttgactt gtgaccgaag
    13081 agttggtagg agaacaccgt ttataagagg ttgtgacaaa agtcttgtcg caacctcttt
    13141 tttaaccaaa aaagtgcaaa tagtttactt taagtaagag aataattatg atgaataaaa
    13201 aaggtggaac aaatagcata ctatatatat tgaaatgaac actgtatgga tgctccaact
    13261 tgatgaagct aaggaggaat tcgtatgtca gaaaaggaat taatgaaggt agtcggtttt
    13321 tatgaagggt tgccgatcga tgatccaaaa agctttattg atgaaaaaga acagatacct
    13381 gtacccagtt cacgggatct tctggtaaaa gtcaacgcag tatctgttaa tcctgtcgat
    13441 acgaaacttc gccaagataa cgggataaga aatgctttac gtatcttagg ttttgatgga
    13501 gtcgggaaag ttgttgctgt aggtgacgaa gtgcaaaaat tttctgtcgg cgatcgcgtt
    13561 ttttacgcag gtacaacaac aagagcagga agcaatcaag aataccaact agttgacgaa
    13621 cgtattgtcg ctcttgcacc aaaaaatctt tcagatgaag aagctgcagc cttgccgttg
    13681 acctcattga ccgcttatga attgttattt gaaaaatttg gtctaactcc agaagaaaat
    13741 gccaatcgcg ggaaaaaaat cttagtgatc aacggatccg ggggcgtcgg ttcgattttg
    13801 aatcaactag cgcattgggc tggcctagaa gtctacgcga ctgcaagtcc gaagaatttt
    13861 gaatggctga aaaaaacagg tgtagactat ccaatcgatt atcatcacga cttaagtaaa
    13921 agttttcgtg aattaggaat cgatcaagta gagtatgtcg ctgtattatt cgatattacc
    13981 cgctactttg atcaaatcaa gcaccttgtc cgaccatttg gacatgtggg aacgatcgta
    14041 gggatcaaag agccgttaga tataggtgtt tggaaaaacc aatctatcag cttcgactgg
    14101 gagtatatgt tcgctaaaac agactataac tatgaaatcg aaacgcaagg agaagcttta
    14161 gcgcacattg ctgctttagc aaatgaaggg attatccgca gtacattgag caaggtctat
    14221 tcagatggga tgaatgcgaa gaacttgaaa caagcaacca aagacgtaga aacaggacat
    14281 atgttaggaa aagtcgttgt tagtggtccg ttcaacggat aagcaagaat acgccgattt
    14341 ttttcttatt tgactttgat aaaaacaaga gcgcatgaca gtaatttgcc ataagctctt
    14401 gtttttttat ttataataag cgaatcaacg aaagggaaag aattcctgcc aagacgatca
    14461 ccaataagct ttttgaaaga atagcagcta aaagtgtagg aactgaagcc aaagcatttt
    14521 cgagattgat ctgcggcaga tgtccgatat tttgggtaaa tagactgctg aaccataaag
    14581 cagacataat gacgatcggg acaaaactta aatactcaag caacggttgc ggtaaatcga
    14641 attttttcag taagacaaat ggtaggaccc tagaaagcca agttgctaaa gtacaaccta
    14701 aaatagtcag caatgtaaac tcaaaagaag gcatgtttga tccacacccc caatccgcac
    14761 ccaatcagtg ttacaactac aatcactaag ttgcttggga taaaaatcag tccgacatat
    14821 accagaatca aagtgagacc aatcaggatc aactgtaatc gtttagaggt gtttctatca
    14881 gaaatgactt gaagataaag taacccgata aacatagcga cgatggcgaa atccaaacca
    14941 aactttttcg gatcagtaat aaaattccct aaaagtgcgc caacaagaga agaacccacc
    15001 cacgtcaaat aagaaatcca attagctgta ttcatccaac gaaagtttaa cttttgtcca
    15061 gtatagttca atttattcat tcccagtgcg aaactttcat ctgtcagcaa tgtgccaatc
    15121 agcagatttt gaagcagccg atttttcttg aaataaggag caatcgtcat actcatcaga
    15181 atcattcggg aattgactag aaaggtcgag aaaacgatgg aaagcaatgg gctgtgacta
    15241 gcaagcatgc tgactgtgat aaattgggca gaaccagcat agaccagcag ggacatcaac
    15301 gtgacgacta gtggatgaaa tccggcagct ttcccaacga taccaaatgc aagtccaata
    15361 ccgatataac cgaaaacggt aggtaacgtg tctttgatcg cagtttttat atctaacttt
    15421 tcatccaata acaaaactcc tttagcacga cataaatttt cataattgtc gttaaacttg
    15481 tgaatattct aaatttttaa tgagcatttg tcaatggtgg gattgaaacg taaagaacta
    15541 gcgggtcgca gatttacacg tattgtttcg gtgggaggag gcgcgaaaaa tcttgcatgg
    15601 ctacaaatcc aagctgatat tttcgatgca acggtctatt gtttaagtat ggaggaagga
    15661 cctgcattag gcgcaacaat gattgctgca ctagggttag ggtggttcga cacacctgaa
    15721 agttgttgtc agaattttat ccaatataca gatgcagcgg aacctctctt acaaaatgta
    15781 gaaaagtatc ataaaatcta tcaacaatgc cggaaaatct atccagcaac gaaggaaatt
    15841 tgtcatgact tataaaacaa aacgcgtacc taatagtcaa atgactaata ttgggtacgc
    15901 gttaagtctt ctttacttca tcctttgcat gattcgtgtt tttaaagcaa ggtctgctgt
    15961 atttcttgtg gatttaggat ggttgttatc gtttacttgc tatttttcat tgaaatcgtc
    16021 tttctttacc gtttctttag ttttttatct atttttttct tttggttaga aaattctttt
    16081 tcccgaaagt cttctttcag gatttttata gagtagcgtt ttgaataaga ggcgtcagta
    16141 tgatcgactt ggcagacgaa ccaagagaac atttgccttt tttctgcatg aggtgcttct
    16201 gctaataatt ctttccataa ataataacag tcctctatca gaagaagggt tcgttcatcc
    16261 tcaggctccc aatagaatgt ttcgagagta gcaatcactt cattgatcaa ttcgaatgcg
    16321 ggaagatagg cattttgctg aataagcgat tcaactgctt cttggatgaa ctttcgcatc
    16381 tccatgatga acttcgctgt tttcctgttt ttgtttttct tgataatatg atcgtattta
    16441 tggatgatag ttgttaattt atctgaataa tttattgcat gaaatggcat cttaactttg
    16501 aagcgttcga taaagtgcgg atgttctata agaatctctg ttaaaagctc ccgtacaaga
    16561 gaggaactag cggaggaaac aagggacgag agttcggaaa gtgagtagtt ggattttttt
    16621 attggacgtt cttcgtaagc gaataagaca gcagccatgt gtttgcattt ttttccttct
    16681 atggcatata gacaatcaca agtcatgttg gtcttttcat cccaagtgat cactacctta
    16741 taatcttccg tgccagaaac tgttgcggac aagtacttgc ttgtatagtt caaatgacgg
    16801 acaaaaccgt gcagatgata gctgtaaccg cgttccaaaa cgttttcagg aaaatactca
    16861 agccaattat tcatctagtt ctcctttcat gaaacactta tttatattta gtataaagaa
    16921 acgtttaag
//
LOCUS       DAZKCN010000056        16399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000056 DAZKCN010000000
VERSION     DAZKCN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.057"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8K3_001726"
     CDS             complement(296..1024)
                     /locus_tag="ACG8K3_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145598.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8K3_001727"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8K3_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP9145599.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8K3_001728"
     CDS             2805..3743
                     /locus_tag="ACG8K3_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145600.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8K3_001729"
     CDS             3755..4666
                     /locus_tag="ACG8K3_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9145601.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8K3_001730"
     CDS             4690..6162
                     /locus_tag="ACG8K3_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9145602.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="ACG8K3_001731"
     CDS             6214..6825
                     /locus_tag="ACG8K3_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP9145603.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="ACG8K3_001732"
     CDS             6855..8768
                     /locus_tag="ACG8K3_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFP9145604.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="ACG8K3_001733"
     CDS             8896..11877
                     /locus_tag="ACG8K3_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP9145605.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="ACG8K3_001734"
     CDS             11874..13535
                     /locus_tag="ACG8K3_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFP9145606.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="ACG8K3_001735"
     CDS             13575..14507
                     /locus_tag="ACG8K3_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9145607.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14867..>16399)
                     /gene="guaA"
                     /locus_tag="ACG8K3_001736"
     CDS             complement(14867..>16399)
                     /gene="guaA"
                     /locus_tag="ACG8K3_001736"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9145608.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         5239 a         2689 c         3301 g         5170 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgctttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc gaacaagaga
    14641 ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt aggagcaatt
    14701 ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc ccttttttgg
    14761 taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt caaaagttga
    14821 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcca
    14881 ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt gcgacttcat
    14941 ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca aagtcagcgg
    15001 tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac gtacggccat
    15061 ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc
    15121 gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca cggacgattt
    15181 ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt ccagggaatg
    15241 gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca cgtacttcgt
    15301 ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca ggtaatccac
    15361 ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg atcacatcgg
    15421 tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt ttggttgctt
    15481 catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct tctggatcag
    15541 acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata atgttcaagc
    15601 caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc aacaatacgt
    15661 gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa
    15721 cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg acttgttcac
    15781 gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca gtacattggc
    15841 agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg cgaacttctg
    15901 catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg atggggcaat
    15961 ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac
    16021 tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct gcagaagtga
    16081 tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt ccgcctaatt
    16141 tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa
    16201 tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg ccagaaagga
    16261 tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg ctcatcaact
    16321 ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac tgactaccgt
    16381 aatcaagtac gataatttt
//
LOCUS       DAZKCN010000057        16091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000057 DAZKCN010000000
VERSION     DAZKCN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.058"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..1945)
                     /locus_tag="ACG8K3_001737"
     CDS             complement(218..1945)
                     /locus_tag="ACG8K3_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP9145609.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1938..3131)
                     /locus_tag="ACG8K3_001738"
     CDS             complement(1938..3131)
                     /locus_tag="ACG8K3_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9145610.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3318..3962)
                     /locus_tag="ACG8K3_001739"
     CDS             complement(3318..3962)
                     /locus_tag="ACG8K3_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9145611.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4056..4190)
                     /locus_tag="ACG8K3_001740"
     CDS             complement(4056..4190)
                     /locus_tag="ACG8K3_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP9145612.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4192..6324)
                     /gene="feoB"
                     /locus_tag="ACG8K3_001741"
     CDS             complement(4192..6324)
                     /gene="feoB"
                     /locus_tag="ACG8K3_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP9145613.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6321..6794)
                     /locus_tag="ACG8K3_001742"
     CDS             complement(6321..6794)
                     /locus_tag="ACG8K3_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP9145614.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7195..7419
                     /gene="nrdH"
                     /locus_tag="ACG8K3_001743"
     CDS             7195..7419
                     /gene="nrdH"
                     /locus_tag="ACG8K3_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9145615.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7578..9737
                     /gene="nrdE"
                     /locus_tag="ACG8K3_001744"
     CDS             7578..9737
                     /gene="nrdE"
                     /locus_tag="ACG8K3_001744"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9145616.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9787..10752
                     /gene="nrdF"
                     /locus_tag="ACG8K3_001745"
     CDS             9787..10752
                     /gene="nrdF"
                     /locus_tag="ACG8K3_001745"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9145617.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10841..11980)
                     /locus_tag="ACG8K3_001746"
     CDS             complement(10841..11980)
                     /locus_tag="ACG8K3_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9145618.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12289..13002)
                     /locus_tag="ACG8K3_001747"
     CDS             complement(12289..13002)
                     /locus_tag="ACG8K3_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9145619.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13256..13957)
                     /gene="nagB"
                     /locus_tag="ACG8K3_001748"
     CDS             complement(13256..13957)
                     /gene="nagB"
                     /locus_tag="ACG8K3_001748"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9145620.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14191..15723
                     /locus_tag="ACG8K3_001749"
     CDS             14191..15723
                     /locus_tag="ACG8K3_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9145621.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15914..>16091)
                     /locus_tag="ACG8K3_001750"
     CDS             complement(15914..>16091)
                     /locus_tag="ACG8K3_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9145622.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         4980 a         3332 c         2822 g         4957 t
ORIGIN      
        1 aataaacttt aatcacatta gtctttcata cctacgatcg atctctttga ctcgcaccca
       61 taaattcata atagtttgtc ggcgtctttg aacgtttcga agatgagcta cgcattctct
      121 ttttttatag acaaaaaaga tagagacgaa catggagctt ccaaatttgg atatttgatc
      181 caattttgga agctccattt atttcgtgtc tgtcttttta ttttttccag ctttcgacta
      241 attcttggtt ctctttgatc cagtcttttg ctgcttgctg tggatctttt ccattgttga
      301 tttccaacat aactttttcc atatcttttt ctgaccaatg gaaattgtct aatactttgt
      361 aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc tgctcttcgc
      421 ttcccattcc gttttcagga tcttctaagt atttcagatc atatttggca aacatccaat
      481 gaggtgtcca tcctgtaact acaattggtt cgtgtttttt gatcgcttga cctagtgcaa
      541 cggtcattgc tcctgaagaa gatgtctcta ctttccagtc tttcaagttg tcatattttt
      601 ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg ctcgattcct atgattttct
      661 tacctgcctg atcggttaac tcatcgatcg aatttacatc catgtaggct ggcactgcta
      721 aaccaacttt tgctcctgtc aaattggcac ctaggtcttc gacttgatct ttatattgtg
      781 catattgtga accatgagtt ttaggtagcc aagctgaaac catggcatct gactcgccat
      841 ttgaaacaga tttccacatg attgaattat ctaatggcgt catcgttaca tcatagccca
      901 tttgtttcaa tacttcaccg actacatttg ttgaagcaac ttctgtatcc cattcaacat
      961 aagataagtt gatacgtttt gtttctttgg cagatgaaaa actccctata ccaatcgcac
     1021 ctaagatcac tactactaca gcaccaatga tcagtccttg acgtttttta cttttctgtg
     1081 ttgttgctcc tgcagctttt ttattgtttg gctggttcaa atgctgtgta aaacggtcaa
     1141 tgatgattgc taagataact aatgcaaccc cgttgacaaa tccattcccg acttgtgctc
     1201 tttgcaatgc tgataatacg ccgcgtccta agccgggtgc accgatcata gaggcaataa
     1261 cgaccattga aagtgacagc attgttgttt gattgatccc tgccatgatc gtacttttag
     1321 ctaaaggcaa ttctagtttg aatagttttt gacgacttgt gctcccgaat gaatcagaag
     1381 cttctactaa ttctttagga acttgtcgaa tccctaaatt cgtaaaacga acggttggtg
     1441 gcagagcaaa gatgactgag gcaaataccc ctggaaccat cccgataccg aaaaatgcaa
     1501 ctgctggaat caaataaacg aatcctggca ttgtctgcat aaagtcaagg atcggtttga
     1561 taatactttg ggctttactg ctttttgcca tcaagatccc taatggtacc ccaatgatga
     1621 tcgagatcac actagataaa agaacaagtg tgacagtatt catcaaatca ttccatagac
     1681 cttggttata aataaaccat agaccgatta atgtgaacaa cgtaagtcct ggacgtttct
     1741 tagaaataaa gaaagctgct accgtcaata acaagataaa caaaggagct ggaatcacta
     1801 acagtaaatt tgtaatcccc gacatcactg cctgtccaat cgtttgtaaa aatgaaaata
     1861 ggccagaaaa agttgtcgta aaccattctg tgatattttc tacccagttt gccactggta
     1921 attgatagtt caatagatta ttcatgttca ctcacctcgc ttgtttctgc taatgcttca
     1981 atcacactac ctcggatgac gacacctttt aattttccat cctctacaac tgcaagtgga
     2041 gtcggtgagt cataaataat attgaaaata tcacttacca ccatgtcttt cccaatcacc
     2101 gtcacatttt catctaggaa gtcgatcaat ggacgttttt gcttcctagc ttctaacgct
     2161 tgatctgcag tgatgatccc tttcaggtgt ctctttctgt caacagccat cagcatgctg
     2221 acttcttcat tacgcatcct tgtcaatgca acgtttggtc catcagattc gatattcgtc
     2281 gtcaatgcag gtaccatgat gttttgagcc gtaagcactt tggaacgatc gacttcttcc
     2341 acaaattcac gtacataatc atttgctgga ttggtcaata tttcttcacc tgttccgatc
     2401 tgcatgattt cgccgtcttt catcaaggcg attctgtccc caatccgtaa agcttcattc
     2461 aaatcatggg taatgaaaat aatcgttttt tgaacattgg cttgcaggtc cagtaattcg
     2521 tcttgcattt ctcgccggat cagcggatca agagccgaga acgcttcgtc catcagcaag
     2581 atctcaggat catttgctaa agctctggct aggccaaccc gttgttgcat cccaccagat
     2641 aactgactag gatattggtc tttaaatgaa agcaaactag aattttctaa tgctttttct
     2701 gctttttctt gtcgttcttc tttaggtaca ccacggactt ctaacccgta ttctgtattt
     2761 tccaagatcg ttcgatgtgg aaacagaccg aagttttgga aaaccatatt gattttatga
     2821 cgacggactt ctcgcaattc ttctttgctt aacttcgata catcttctcc atcaatatag
     2881 atactgcccg aagttggctc aatcagacga ttcaatagac ggatcaatgt ggatttccca
     2941 ctcccggaaa gccccatgat cacgaagatt tctccttctt ttacgtcaaa attcacgtca
     3001 tacacaccaa ccgttgcacc ggttttcttt aaaatctccg ttttcggctg gtgatcattc
     3061 atcatatcta aagcggcttg tgtcttttta ccaaatatct ttgttaaatg ttttacttct
     3121 acttttacca aacaaattct ccttttttat gtaaattcat ttttctttct gaaaaaagtg
     3181 accacactat gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat ttgcttgtcc
     3241 aaactgaaaa aaacttggct aaatctttgt aaataaaagg tttagccaag ttaatatttt
     3301 tttaatattt cacacaatca ttctgcaagc tgatgctccg ggtaaaaaaa gtttgtcata
     3361 cgctgtagtg tatattcatt tccaataaaa tgaacgatgt cccctgccgt aaatttagca
     3421 taggggccag gtgaaacaag cagttcttca ttatgagaaa ttgcgataac tgtggcgcca
     3481 gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca gatgcattgc tttttctgat
     3541 aaagataatt caaacggaag taaaggattc atcttatgta ttcgcttcgt ttgattgacc
     3601 agctgatcca ataattgaga aaaatgattc agctcatctt gttgcttctc cacacttgcc
     3661 atgatttctg cttttgtatc ttggatggat tgtacatctt gatattgcaa taggaaatct
     3721 tttgcttttt ccttcgaagc aacaaatgcc ccacttccat gccgtacttc catgattcca
     3781 agatctacca gcacattgat agccttgcgg gtagtctcag gagaagcatt gtacgtactg
     3841 gcaagtgttg agcgggcatg gatcttctca cccactttat atcggttttc aacgatacgt
     3901 tcggctaaat caacagcaat ctgctggtag cgcgggcgga cgattttttt ctttccagac
     3961 attttctttc ctctttcttt ttttactttc cctcatctta ctatgctcta gcaaaataag
     4021 caaaaaaggg gagaatagcc tcttgcaacg actgatcatc gcttgatctg ttcctttttt
     4081 actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg attttaaacg agtatagaca
     4141 attgcgcctg cacttccaaa gattaacaca cttaataaga atgtcgccat cctattcctc
     4201 tccttctaat gacaatatag tataaaactg gttcttggat tttggctgac ggaacaaaac
     4261 ataaacgagt cctatcatca gtaaaatagc aagatacgtc cctaacgcta cctgtccacc
     4321 ctccaataag tgtcccaact gataaatcac aaaactaact aaataagcta aaccgcattg
     4381 atagcctatg gcgatccaag tccatttctt ggcattcatt tcccgatgga tagccccaat
     4441 tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac aatgaataag ctgctgcggg
     4501 agtatatgtg tgttgtagca cttgccagat ttctctgcca ttttcggata cttccgcatg
     4561 accaaacaaa ataccaaatg tgccaataat attttcttta gcgaccaatc ctgtaatcgc
     4621 tgcgactgtt ccttgccagt ttccccaacc aagcggctga aaaacaactg ccaggattcg
     4681 cccgaaaaag gctaagatac tttggtcttc agaaacaggt tgagcataaa aattataaga
     4741 agagctgaac cagatcacga tactagaaac aaagataatc gttcctgctt ttttaacaaa
     4801 agacttgctg cggtcatagg tttgtcttag gaccgctgac cattgaggaa aatgatatgc
     4861 gggcaattcc atgataaaag gagctgtatc tccagcaaac aaacgtgttt ttttcaagct
     4921 gattccagag aaaataattg cagtcatacc aagaaaatag gcagaaggag ctacccaaga
     4981 ttgatgagga aaaaaggccc ctgctactaa ggcaataatc ggcaactttg cagaacaagg
     5041 catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa tcgtgcgact
     5101 ggccatgacg cctggcactc cacatcctgt agcaatcagc ataggaatga aggattttcc
     5161 tgaaagaccg aatttcctga aaagtcgatc catgacaaaa gcgatcctcg ccatatatcc
     5221 gcagtcttcc agaaagctca agcataaaaa caatataatc aattgcggta gaaatcctaa
     5281 cactgctccc acgcctgcga tgatgccgtt caagatcagc tcctgcatcc atcccgctac
     5341 ttgccattca gctaacaatc tgccaacatg ttctggtaac catgtaccaa acaatgtgtc
     5401 attgatccaa tctgtcccca ttgttcctac tgtttgaata gccagataat agatcagcca
     5461 catcacaaat gcgaagatcg gcaaggctaa ccaacgattc gttgcaatct gatcgatttt
     5521 gtcactcatc gataattgga aagaatcatt tgacacaaca cagagtgtac atagttgggt
     5581 aataaattca taacgttcat tgaccagaat cgtctcacta tcgtcgtcca atagtttctc
     5641 tgttaactgg atcagttgct cgatctcttt ttgctgagaa actgctatct cgtaatcctc
     5701 tcttaccttc tgatctcgtt caaaatattt cattgcatac cagcgtttct tgccgctttg
     5761 ttctcctagt acatcttcaa tttcatgtaa agctgcttct aaacgtggat catatgtaca
     5821 gggaacagga atagaaggcc gcttttttgc agactcctga atcttttcga ttagtttttc
     5881 gattccttgc tttttcaata cgctgattgg taaaactggt acaccaagac cataggaaag
     5941 cttttctaga ttgatttttt ttccttcttt tttgatgaca tccatcatat tcagaccaat
     6001 gatcatagga atgtctaaat ccaataactg cgtagttaaa taaagatttc gttctaaatt
     6061 ggtagcatca ataatgttga tgatcagttc aggctgttcc tgaaccaaat aatctcttgc
     6121 aatcacttct tctggtgtat aaggagacaa agaatagatg ccgggtaggt cctgaatatg
     6181 aatgttcgga ttttttttca acgatccttc ttttttttct actgttacgc ccggccaatt
     6241 ccctacatat tgattcgaac cagtcaagag attgaacaag ctagttttcc cgctgtttgg
     6301 atttccgact aacgcgatat tcattctttt tcctcccttt gtatcaagat tagttcggcc
     6361 tctgactttc gtaacgtcag ttcataccct cgcactgtca gttcgattgg atctcccaga
     6421 ggggctagtt ttcggataac tatttttaca tttcgcgtca atcccatatc cattaaccgt
     6481 cgcttcactg cgccttgtcc gtggattgct acgacgatcc cagtctctcc tgcttttaat
     6541 tgatcaagag aagtccagct ttcagaggaa gcagtctttt tattgacaaa gatttgacct
     6601 agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc attgttgcct
     6661 gataaattga cgacgacgat tttacttccg gcaacgatcc cgatattttg caaatggcgt
     6721 tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaaattctg ctttgcctgc
     6781 gctaaggtct gcatattgat tcctctcttg tgaaataaat catgtattaa aatcattcat
     6841 tatatgttct aattttctta cgtgttacgc aaataaatga gaatcattct cacatagaat
     6901 actctcggta attgagaatg tcaattatac ttgtgatatt atgagcgaat ctttagtatt
     6961 gatagataaa aatgagaaaa aaatatttct tcatagtttt ccacaatttt ttctctatta
     7021 aatttgctat tttcccacac gatacttgtt ttcagctctt ttttcaaaaa cacaaaactt
     7081 agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt ttttactaag
     7141 agttttatac tatctattat agataataag aatgtgttag aggaggcact caccatgaac
     7201 gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg gtttttaaca
     7261 gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc agtagactgg
     7321 ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga aacagttatc
     7381 ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa gaatcaaaac
     7441 gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa cttccaaatc
     7501 cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca aatacgaaat
     7561 gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat ttcaagctga
     7621 ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat aaagaagctt
     7681 tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcca tctattatgg
     7741 aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt caattgtatt
     7801 caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt tctttcaaat
     7861 cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac gacggttccg
     7921 cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt gcgaacgggg
     7981 atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat cagccagcta
     8041 caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct tgtttcttga
     8101 ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct ttgcaattgt
     8161 cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc ggcgctccta
     8221 tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta ttcgaagata
     8281 gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt tatttgaacg
     8341 ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca gatgaaaaga
     8401 ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat gaattgacac
     8461 gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa tatggtgtcc
     8521 cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat ccaaacattc
     8581 gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta caacaagaat
     8641 ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca atcgatggca
     8701 agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct tctgtgatta
     8761 acggcaaaca ggaatatgaa gtattaggaa cagatatctc atgtaactta ggctcgacaa
     8821 atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg atgactcgtg
     8881 cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc caaaacggaa
     8941 ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat cttgcaaaag
     9001 aacaaatgga atatggctca gaggattcat tagactttac aaatatttac ttcatgctgt
     9061 tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa caagttttcc
     9121 ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac gtaactggag
     9181 aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt atcccaactg
     9241 ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat catcaaaatc
     9301 gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt gcaagtattc
     9361 atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa atttattacc
     9421 cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat gatatggaca
     9481 tgcgtaaagt aatcgatgtt tatgcaacgg cacaacaaca cgtcgatcaa ggcatgagtt
     9541 tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag acaacaacga
     9601 agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa ggaatcaaat
     9661 ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc aatcaatgtg
     9721 aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa ttaaaggagc
     9781 attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga agatatcatt
     9841 gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg tatcccactt
     9901 tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac agtaggacat
     9961 gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat ggatcagttg
    10021 cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca attcatggaa
    10081 tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa aaaagaaatc
    10141 gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc tgagcgtatc
    10201 aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt ctttttagaa
    10261 acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa caacaaattg
    10321 gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca tggaacctac
    10381 atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca agaaacgtta
    10441 aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg ttatacagaa
    10501 gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg ctataatgcc
    10561 aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc caatgatgtc
    10621 aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt cttctcacaa
    10681 gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga ttatttgatt
    10741 ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa agagtttagc
    10801 gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt gtttcttcca
    10861 gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat aaatagattt
    10921 tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa atcagcatgc
    10981 ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc aaaggatgga
    11041 tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat tgttggatca
    11101 ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa ccgacaatga
    11161 ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg attccgcaaa
    11221 tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa aagctcatta
    11281 caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat gtttcgttta
    11341 attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag tgagacaacg
    11401 catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga acggtaaata
    11461 acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca gataaactga
    11521 ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat tgttcttcca
    11581 aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg cttgtttcat
    11641 ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac tgaggcacta
    11701 cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat aaaaaaccga
    11761 acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac atataataga
    11821 taaaaccagc aacaagcaat ggtaaaaaaa ttgccgaaag cattttcaat attggactaa
    11881 aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact agctccactg
    11941 tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc caaggtcctt
    12001 tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca tttgcttaaa
    12061 aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca aaaacacggt
    12121 ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaacgg tgttcaaaag
    12181 cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt tttatttggc
    12241 tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt attgcgcaat
    12301 actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga tcacatcggc
    12361 ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc ggtagtcatc
    12421 aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt caaaataatc
    12481 ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact ttttagtcgt
    12541 aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga taaaactgat
    12601 gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa ttgcccataa
    12661 agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg ttcccgcgcc
    12721 taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg aacgaatcgt
    12781 tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga tctcaacttg
    12841 gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca tacctgctgg
    12901 acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag cgatccctaa
    12961 acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac cctccagtcc
    13021 aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc ttacgaaaaa
    13081 aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt tcaatcagct
    13141 gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc tttgtcttct
    13201 ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt gaaaattaca
    13261 acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt tgcaatacac
    13321 ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca tcagctttgc
    13381 tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg attcccattg
    13441 aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt gcattgattg
    13501 ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca ggttcattga
    13561 aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga tgtgcatcga
    13621 tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca tctggtacaa
    13681 acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga cggtagcttt
    13741 gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc atttcactga
    13801 aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt cctgttgcca
    13861 atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat gctttttttc
    13921 cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct ttatattttg
    13981 gtatagccca attatagcat tctttttttc tttttacaat aattagtata gaccaaattg
    14041 catttttttc attttcttga caataaatag gaatccgtgt taggtttcat aagatggttc
    14101 aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga cgctttatgt
    14161 ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa caacattacc
    14221 cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa tgctggcgaa
    14281 catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa aatcatcact
    14341 ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca caaaggttac
    14401 ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct aaaaactgcg
    14461 tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac atatagcgag
    14521 accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt agaccaagga
    14581 gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg gatcgatgtc
    14641 ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa ggtgatttta
    14701 gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac caatcactta
    14761 gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg tgcgtatatc
    14821 gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc agacatcgag
    14881 tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca aaaggaacaa
    14941 aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga aaagaccgaa
    15001 gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag ccgacaaaaa
    15061 caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc tgtctttgat
    15121 tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt agtcatcgga
    15181 tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga aaatattgcc
    15241 attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat cggtgagatt
    15301 gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta cttttcacaa
    15361 gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag tcaatttcca
    15421 aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt taatcaatta
    15481 gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa acttgcacag
    15541 ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca tatcgatcaa
    15601 gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt aatcatcgtc
    15661 tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat tgaaccaaaa
    15721 taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg tcacaactcc
    15781 ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa aaaacggcaa
    15841 aatatgagga gctattttta tttggctatt cttattactt gatgcagaac agcttttcac
    15901 aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa acaactgttt
    15961 gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct aaccagccat
    16021 actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata aagtacataa
    16081 acataaatac a
//
LOCUS       DAZKCN010000058        15997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000058 DAZKCN010000000
VERSION     DAZKCN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.059"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(472..1578)
                     /locus_tag="ACG8K3_001751"
     CDS             complement(472..1578)
                     /locus_tag="ACG8K3_001751"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9145623.1"
                     /translation="MKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRDVPPLDPKI
                     KSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYEGGLYVLCK
                     RHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAEFPELNAKS
                     YVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMDVRKKVHFT
                     IAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAIFPYKEIYG
                     SGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKYVNLSPAEK
                     SKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(1589..2362)
                     /locus_tag="ACG8K3_001752"
     CDS             complement(1589..2362)
                     /locus_tag="ACG8K3_001752"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HFP9145624.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(2376..2831)
                     /locus_tag="ACG8K3_001753"
     CDS             complement(2376..2831)
                     /locus_tag="ACG8K3_001753"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9145625.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(3030..3251)
                     /locus_tag="ACG8K3_001754"
     CDS             complement(3030..3251)
                     /locus_tag="ACG8K3_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145626.1"
                     /translation="MEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIGNSVE
                     FVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            complement(3253..4359)
                     /locus_tag="ACG8K3_001755"
     CDS             complement(3253..4359)
                     /locus_tag="ACG8K3_001755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9145627.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(4377..5138)
                     /locus_tag="ACG8K3_001756"
     CDS             complement(4377..5138)
                     /locus_tag="ACG8K3_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFP9145628.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(5214..6212)
                     /locus_tag="ACG8K3_001757"
     CDS             complement(5214..6212)
                     /locus_tag="ACG8K3_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9145629.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(6231..6884)
                     /locus_tag="ACG8K3_001758"
     CDS             complement(6231..6884)
                     /locus_tag="ACG8K3_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9145630.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(6894..7904)
                     /locus_tag="ACG8K3_001759"
     CDS             complement(6894..7904)
                     /locus_tag="ACG8K3_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFP9145631.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(7923..9173)
                     /locus_tag="ACG8K3_001760"
     CDS             complement(7923..9173)
                     /locus_tag="ACG8K3_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFP9145632.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(9680..9960)
                     /locus_tag="ACG8K3_001761"
                     /pseudo
     CDS             complement(9680..9960)
                     /locus_tag="ACG8K3_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697748.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10519..10629)
                     /locus_tag="ACG8K3_001762"
     CDS             complement(10519..10629)
                     /locus_tag="ACG8K3_001762"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFP9145633.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSETLMEWYQLIIT"
     gene            complement(11035..11724)
                     /locus_tag="ACG8K3_001763"
     CDS             complement(11035..11724)
                     /locus_tag="ACG8K3_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9145634.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(11769..12533)
                     /locus_tag="ACG8K3_001764"
     CDS             complement(11769..12533)
                     /locus_tag="ACG8K3_001764"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP9145635.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(12561..13259)
                     /locus_tag="ACG8K3_001765"
     CDS             complement(12561..13259)
                     /locus_tag="ACG8K3_001765"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP9145636.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(13272..14051)
                     /locus_tag="ACG8K3_001766"
     CDS             complement(13272..14051)
                     /locus_tag="ACG8K3_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP9145637.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(14067..15011)
                     /locus_tag="ACG8K3_001767"
     CDS             complement(14067..15011)
                     /locus_tag="ACG8K3_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9145638.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(15052..15831)
                     /locus_tag="ACG8K3_001768"
     CDS             complement(15052..15831)
                     /locus_tag="ACG8K3_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145639.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         4627 a         3547 c         2505 g         5318 t
ORIGIN      
        1 gagaagtact cctgaaaatt tctgaataag tgaaatagat gttctaagaa tgtatgaaag
       61 aaatgttaca ccacatatcg tatttagcat tgaatccaaa ttccacgctt ggttaattaa
      121 agtacagaac aatggatacg ccaataatac atagtataaa ctcatacttt ccctagattt
      181 aacccatatt gtcttatctg agtagatgta agctgctatt ataacaacac aaatcagtaa
      241 agtgcagaaa tttaaatatc tattttttct aaatgctggt aatgaggcta ttaacaaaca
      301 tactattgct agaatgactg tcacaagtct aacgttcatc gtttctccca accagccgcc
      361 caaaggcttc aatatgcttg atgagataat cattagaaag accattagaa actgtatgat
      421 tttccattta ctaatctgca tgttaattat tattcctttc taattgtttt actactcatt
      481 caaaatttgt ttgtatgcct cgtatgtgag ttttgcagat gccgcccagg tatacttgcg
      541 gtttacaagc tcatatatat catcctctat tttgcttttc tcagctgggc taaggttcac
      601 atactttaca attgtgtctg ctaggctctt agaattcaaa ttttcgaaaa gtaaccctat
      661 ctttccttcc tcggtttctt ccataaaagc aggtacatta ctaacaatag ttggtaagca
      721 ataagtgtat gccataagaa gcgctccact tccatagatt tctttatatg gaaatattgc
      781 ggcatctgca tttctgaaca tactgtccat ttcttcatca gaaattctct ctggcttata
      841 tagaacacaa tctgaaacct ttaaatcttc aatcattttt ttatagtcag tactatcctg
      901 tttaggatgt tgttgacctg ctatggtaaa atgaactttc tttcttacat ccattggaag
      961 ttctgtgatt gcattaagca gtacatcaat tcccttatat ggacgaattt gtccaaacaa
     1021 gataaattca aacttatctt tatcatacgc tctagatata tgtttaacat ctccatagat
     1081 agcatgtgga caaacatatg actttgcatt taattctggg aattcagcat ataaatgctg
     1141 ttctgttact cgattgtgaa caatgaaacc atcacaattt gaatatatat taaagtgctc
     1201 ttggcgatcc ttttcacgaa cctcatgtgg taacacatta tgaactgtca taattaattt
     1261 tttatagtgt ctcttacata aaacgtacaa tccgccttca tatttgagac ttttcatcaa
     1321 ttgcaaatga acaatatcat aatcaccaaa tcttatttcc ttccaaatta agaacaagct
     1381 tttcagaaaa acaagtgccg aaaacacttt atttgtactt ttatgataca aaatatgttt
     1441 gctcttaatt ttaggatcta aaggaggaac atcacgctta cacagcaatg ttaaatcaac
     1501 atattgatta agttctttag aaagatcaaa cgaataccaa ttctctggca tttggctttc
     1561 acacatcaat accttcataa tactcacctt ataaccaatc aacagaaaat tctgctttca
     1621 gttttccttt aattttacgt tttgctataa acatttccag tatctctcgc attttcgtct
     1681 gatattttgt cgttttacca taaagcgagt gattgtcccc tgttcctaat acatatactg
     1741 ctgctttgaa tggaagaggt tttaatggac gcccttgctc ttcacatgct ggaacaacgc
     1801 ccccatgtcg ttttctaatg atccatggat tatcatcaca atttgctgtc ataacttccg
     1861 gatatgcctc aggcaggtct tccactgcat attttactat acacgcactt cctgatgaga
     1921 actttggtag aaccttcata taatcgtctc caacaaagta cacgtatccc gttttgacat
     1981 aaaatccatt ttcctgggga tgggaattca catattccgc caaatgagaa cttacaagat
     2041 catctgcgtc aaccatcata gcgaaaccgc cgcccaactc tcgcaaacgc attgcgagag
     2101 catgtgtttt ataaccctta tcaatcattt tttcgtcttg atttgtggga ataggggcat
     2161 ccttttcgct aacttgaata aattcaactc tgtcatcata ttcatgcgtg agtttaggaa
     2221 tttcatggca agcaacaatg attttgaaat caggatcagt ctgattataa cacgaccata
     2281 atgttcggtt aaataaggta ctaaccatct cccaattgtt tgctgccttc gcactcatta
     2341 aaggaataac aaaatacaac atacaatctc tccttttagt aacgattcaa actacaataa
     2401 tcatttacat caatatcttt tttcaatact ctagccggaa cgcctcctac cgaacaatta
     2461 ggttcaacat cattaatgac cacagcatta gcccctacta ttacattgtc ccctattgtg
     2521 atattaccta aaatttttgc gccagtagcg atatatacat tgtttccaat tgtaggcata
     2581 ccaggcttat tggatctacc accagtcagt acattcgttc cgattataac gtgattacct
     2641 atttttgtat cttttccaat tacacaacct atgccaccgt ataagaactt acaattatct
     2701 ccaatttctg tttgatatga aattgagcta ttatgaagta cataaattag tgcctttaca
     2761 atcttggcaa aaaactccat tttgtgtaag taaaaccatc gttctacttt gtataattta
     2821 tatgcattca tgttttatct ccccatcttt ttagcaggta ccccccacac ttcgtagggt
     2881 ggaatagacc tcaataccac tgtatttgca gcaactacag caccttcccc aacgcataat
     2941 tctgtgttag atttacaaag aattttagca cccacacata aaacagcgtt cttttttact
     3001 cgaatgatga tacgttcatt actatctgct caaccacacg tcacattctg atagacttta
     3061 acaccatctt caaggattgt gtcagaatga atcacagtgc ctacagaatt atgaacaaat
     3121 tcaacagagt tccctatctg tgcaccttta ccaatacaac aaccaagaat tttggctatt
     3181 tttctcgtgc aattatcaac tatgtttaaa gcatatcgat tcctcaatgc cattaatttc
     3241 gttacttcca tatcatttat caccttccaa catgctaagc ataatttctc ccgactttga
     3301 ccatctaaat tctgtagtta cacgctgata tgctgcatct atgatatcgt tggtaaatcg
     3361 tccgtctttt aagaatgtca aaatttcatc gtcagattta gccaacattc catatctatt
     3421 atccaaaata atattctgtg ggccaggtgc attaacagca ataatcccga ggcgatagta
     3481 cattgcctct agcaaaacca taccccaaat ttcagtatag tttagattta caaatacatc
     3541 cgataagcaa tataactccc aaatatcttt gttaggaata cgctcaatta tttttactct
     3601 attctgcagt ccaagtaaat ttatctgctg cttcgtttgc tcgagtaaag caccagttcc
     3661 taccatcagc aaataattat gctcatcaag tttattaaat aaactaatca tatctaacgg
     3721 tcttttatcc ttttcaaacc gtcctacata taaaataaca cgaacattgc gaggaatatt
     3781 ccatttttct ctgagagtag taaaatctat tgcttcgtaa ttcttatgta actgttcaaa
     3841 atccacacaa acaggagcaa gtttcacttt atttactcct aattgtacta atttttcaga
     3901 aactcgatca tttttggcaa cacacccatt tttcttataa attttataat ttcgatgcgc
     3961 caatatttca ataattgtct ttatatattt attgccactt tgacttcgcg ttatcccatt
     4021 ataaggtata tattttattc cattttttac agtccatgag tatacatgag gtactgatag
     4081 ttgaaggtcc gcaaattgta ttagaacatc cgcattctta tcaaaattct ttgttgataa
     4141 gaatccattt gatcctacat aacgacactt gatatagtga atcatcgccg tattgcttaa
     4201 ttgctctgtt ccaacttcct catcactttt tacactttta tatacaataa cttcgtggcc
     4261 taattcagct aaccctcgag ctaatcctat ttcctgagaa ttataggacc ccttcttacc
     4321 aaagttattc gtcattgtaa ctactataag tattttcatc tatttttatt gcccccctac
     4381 ttagattcaa tagtatagta gatatacttt atacctccca agatataccg cctccatagt
     4441 ctacgaggtt cctttaccag tctaaataac cactctagac tagctttttc catccattca
     4501 ggagcttttt ccaaagtgcc cgcaaaaaag ttaaagccgg cgcctacacc gaacattact
     4561 gtgttatgga tcttcttctg gtgttcccac atccacattt cttgcttagg cgcaccaata
     4621 ccaacccaca gaaaatctgg atttaagttg ttgatttcct cgcaaatctt gctatcctct
     4681 tcttcattta acggacgaaa tggtggggaa tacattccaa caattttgat tcccgggtaa
     4741 tctctttcta ggtttgcacg aagcttattt aacacttcgt tatcttttcc accatagaaa
     4801 taatgtgttt ttccctgtct aacgccttct tcaaaaacca ttcccataat atctggtgca
     4861 gcacatcgtt cacaggtaaa tcctttgcgc cgtccaattt taacaatagg cataccatct
     4921 attgcagcca ttgtagcctg gctatacatt tcagcaattt tgggattttc ctttgccgaa
     4981 ataatggccg gcactcccac gagatcaaca gtcgcactta ccggagatgt tggcatggaa
     5041 aaaacttccc ttacaaactc gttctttgta gtagcacaaa cacctactat tccacacaca
     5101 gtgaaatcac gttttctttt atacatattc ttttccatct cagtacatcc ttactcatat
     5161 gttcttcctc cgcatcctct aattacacgg aggaagaaca gcttatatac ttattaaccc
     5221 ttaatctgct tttcaatcca attgtaggat tcacgaagac cctcttccaa agaaacagta
     5281 ggctcccaat ccaaaatctc tttcgcctta gaataatctg cgcaacgagc cttatcacct
     5341 tcaggcttag aagtatcaaa gaaaggcttg atgtctttgc cagaaatttc aatgatctta
     5401 tatgcaattt ccttgatgga agtacagtga gaagggccaa tctgaatatg accatgaccc
     5461 caacccttat ccagggcgag caccaatgca tttacgatat cattgacatg aatgaaagca
     5521 cgtccctgct ctccgctacc ccacacatta aaaggctctt caggattatt gattgcttta
     5581 cgaatcagag caggaataac ctgactacgt tcgccaaaat cagtaggtgt gccataaaca
     5641 ttatggagca tcaggataca gcacggaatt ccggtttctt tttccaaata ccccatctcc
     5701 aactggccca tcagtttgct ccagccataa gcagactcag gaagagcagg gaacaactcc
     5761 tcttcacgga gaggctcagg attcaaagta ttctgtctgg tcagagggaa ggagcatacg
     5821 gtaccaacat agataacacc cttgattttc tccttgccag ccttacggca acaatcaaaa
     5881 acattagaat tgatcagatt attgattcgg aacaattcgc cctggttctt aaacacatag
     5941 tcaatgccag ccacaacatc agccagatga actacatact cagtcgtacc aataaccttt
     6001 ttagcctgct cataatcagt cagatcagca ttgaagaaat gagtatccag gtcgatgaca
     6061 ggatgcccat cttcgtcgtt caggtactcc aactttcctc tccacagatt atcagctaca
     6121 taaacatccc agcagccttc cttcaccagt ctcttaacaa gattggagcc aatcataccg
     6181 caaccaccgg taaccataat tgtctttttc atttaacatt cctccaaatt ttattcgttt
     6241 ccaaagaatt tatcttccag catcaaacac caacaatgat atatcggcaa gtgatactct
     6301 tgaatcatgt atgtttcaac ttcaggagcc ttaacacaaa catctgctac cgtggcaagt
     6361 tccccaccct ttgcgcccgt caagccaata acttttatac cgagagccct agcaacaaca
     6421 gttgcgctca taatattctt gctatttccg gacgtcgaaa taccaaggaa tacgtcacca
     6481 gatctaccaa agccaaacag ctgctgtgcg aagacaccca aaccatcgac atcattaata
     6541 taagcagtag acaatgcttc gtgtgcgacc aaaggaattg ccgttaaacc acactcgaga
     6601 tttttggcta attccggacc tcttacaggg tcaattgcaa ctaacttatc ggcaaattcc
     6661 ttacttacag gacgtggcgt cttaaaccgc ttcatcaact cgccagcaat atgttccgag
     6721 tcagcggctg atcctccgtt tcctgccaca agaagtttac caccattcaa atagcattcc
     6781 tccataatta ggtatccatc gataatgtcc tgtttaatcg tctcaagtgt aggatatcgt
     6841 ttaatcaata agtcaatatg tttattaagt ttttccgcta acattgttac tccttatcca
     6901 ttctgttttt caatataagt ttccggcgtg tagtgaataa ctctagttcc accattttca
     6961 aattcaaacg gaatatacat caaatcacgc atagcccaac gaactgcatc ctgtttctca
     7021 ggttgtacat agaaaaccaa gaaacctcca ccacctgcac cgagcaactt tccgcctaaa
     7081 gcacctgcag ccataccctt ttcatatagt tcatcaatgc ttcctgtact aattgctcca
     7141 ccagtacctt ttttcaatct ccaagtaaca tcaagctgtc taccgaaatc atctaaattt
     7201 ctgttcttat cagtaagaat tgcctcagct tcatctacaa gctcatacat ctttttcagt
     7261 cttactctct tatcctctgt gccactgacg ttgttagcct tttgaacatc agaagaaaat
     7321 ctagtgaatc cggtaaagaa catcatcaga ttgttgttaa gctgcttttt acgttcaggc
     7381 gaaataatga caggcagcac ctcataacca tccacattga aattaattcg attgaaccca
     7441 ccataagatg cggcaatctg atcctgccat ccaccggctt cattacacag aactctttca
     7501 agataaatgg cttcatcagc aagtttcttc ttgtcagcat acttaccctc cagtgcataa
     7561 aaagcattca acataccaac tgcaaaagaa ctggatgtac ccagaccaga gcgagcaggt
     7621 aaatcagctt cgtatgtaag ccgaatttca tgcatatcga gcattttcat cgcattacga
     7681 atcgcaggat gctcaatatc ttcaatgcta gttactctct cagtacgaga ataagataac
     7741 tcagcgctgt aatcaaaaaa tctaggcaga tgtctcacat tcacataaca gtacttatca
     7801 aaagtagtag aaagaactgc accgccattc tctttgaaat aatcctccat atccgttcca
     7861 ccgccaaaaa aagacatacg aaacggtgtt tttgtaataa tcataaatac tctctcctaa
     7921 tcttagtctc tctggaagat atcacgggta tatacttttt ccttaatgtc atcaaggcaa
     7981 gcatcgtagc gatttgctat aacagaatca cacattgcct taaactgttc caaatcattc
     8041 acaaccttac tgccaaagaa agtctctcca tctttcaagg tcggctcata aatcacaaca
     8101 gttgcacctt tagcttttac acgcttcata acgccctgga tagaactctg gcggaagtta
     8161 tcagaattgg atttcatggt caaacggtaa acaccaatca caacaggctt ttctttgcca
     8221 gcatcatact gatttgcttc tgcgtaattg taataaccag ccatctgaag tacacgatcc
     8281 gcaataaagt ccttacgagt acggttagat tcaacgattg cctcgatcag attttcagga
     8341 acatctgcgt agttggccag aagctgcttg gtatccttcg gcaggcaata gccaccgtaa
     8401 ccaaaggaag ggttgttgta atgagtacca atacgaggat caaggcaaac tccattaatg
     8461 atctgctgag tgttcaagcc tttcatctct gcataagtgt ccagctcatt gaagtagctg
     8521 acacgaagag ccagatatgt attggcaaac agcttaacag cttctgcttc agtaaagccc
     8581 atgaacaatg tgtccacatt ctctttgatt gcgccttcct gtagcagtgt agcaaaagtg
     8641 tgtgcatcct caaccaaacg ctcatcatcc atatcggtac ccacaataat acgcgaagga
     8701 tacagattgt catacagtgc cttactttca cgcaggaact cagggctgaa aataatattc
     8761 ttgctaccag tctttacacg aacagaagcg gtgtatccaa caggaatggt ggacttaata
     8821 accataatgg catcaggatt tacttccatg accagcctaa taacagcctc aacagcggat
     8881 gtgtcaaaat gttgagtgcg gctattgtag ttagtaggag ccgcaataac aacaaaatcc
     8941 gcagactggt atgctgcctt tgcatccaat gttgcggtca gattcagttc tttctcagcc
     9001 aagtactttt caatataatc atcctggatt ggagatttac ggttattaat cagttcaacc
     9061 ttctctggaa tgatatccac cgctgtaacc ttatggtgct gtgccaacag agtggcaata
     9121 ctcaaaccaa cataaccggt gccggcgact gcaatgttat actgtttctt cataatattt
     9181 cctctttcca ccactgctgt ggtcactact ttcttaacaa aatgctgttt gtgaacagta
     9241 acctgctatt tatataaaac agcccgaaag ctgtgttcat tcatacttaa aacagccagc
     9301 tcctacgccg gcaaattcaa tttttcataa atcccctacc cgtttagggt tatattgcaa
     9361 gtttgagtca tttttattga cctcgtttgg ggttataata cattttcgca ttgaaatgta
     9421 cctacatttt ccactcaacc agtcaccaaa aagtttataa attcacttat taccctctat
     9481 tcttactcca aaggtatgtt taacatcgct ttattgacct attttcaccc caaatggaag
     9541 tttgtttggt acattattgg tctattccca ccccaaactg tctattttga gcgagttttt
     9601 gttttttatc attgcacatt ttcaaggctc aaattctaag cggatttact ggcagggaga
     9661 cattggtaaa tacttgaaca atttcacggc tttcatcatc acatcctacg ataataattc
     9721 taattggata aagattatca ttaataatct actttcacgt aaaaattctg aactaaataa
     9781 aatctattga taatattttt ttcttccact ttttgtatat cctacaggta tagtggattt
     9841 aattatcccc aatgcctctg aagtaacaga catcactaat tcaataacac attcactacc
     9901 atagtttgta ggaacaaaaa tgacaacaaa atctgcttcc ttatgtgatg tttttccatc
     9961 tgtccaaatt ttattaccaa tgaaatctag tttaaaatcc ctcttagaaa ctagttcact
    10021 ctatattttt attcagtttc attagttctg tttttttgtc ttcagtcacc atatacatat
    10081 atatcttaat tatctatcca tttcatccta cgcattcttg cgtaaataca attcactctg
    10141 ttgagctgat ataaatcatt ggtggtacaa agataaaatg ataactgttt ctatacctcc
    10201 aaaaattcca gtaaatatat ctatagatag tttcaaaaaa aattgtctta gtttcataat
    10261 atttaagata gtggtacata ctttataaca acgtactttt ccaaatatta ttttccgtta
    10321 tgaacatcca atatattaca aagtttaaaa gtttagatga actataatct tcatttctgc
    10381 acagtggata ataaattctt atagataaaa aatagcctct tctaaagtca caaatacctt
    10441 actcccacat tttaagtaaa cactctgcag cattctcttt gttataaaat gatagatatt
    10501 cctcaatttt atctcttatt atgttattat cagttgatac cattccatta atgtttcaga
    10561 ataattacaa tattttggga ccggataact cccttttgct cgtaaccact catatccgtt
    10621 aggattcact aaaagtttac cgtttatttt tcgattcttt ttttgaaccg tcccatgaac
    10681 ataccgattc aacaagctaa attataaaaa ttggtgtgct gtcattattt tttggaaaat
    10741 ttattaatgg ctatcaatat acttttgtat catactaaat agctcgtcag ttcctatatc
    10801 ggatatacca atattaaagc atttagatca ttatatttaa gagtggtatc atcccaaaat
    10861 ttcgaatatt ttcttttata taagaataat gatactgaat tgccccattc acctttcttt
    10921 ttttagattt tctacaagtg attcaaaccc tccataatta cagttattac ttttgaaacg
    10981 ataatatgaa catgtgtaat cttttttatt aacaatccat cattctatcc ctaactaata
    11041 tgctccattg ggcttgacaa taacataaaa cgtttttaaa atcaacctca agtcgtacat
    11101 taaacttcga tgactaatat actctaaatc caactcaacc attttatgga aactaacatt
    11161 atttctatca ctaacttgcc acaaacctga acaaccaggt tttacataca agcgttgaag
    11221 atcatatctc gtgtactcag ctacttccct ctccaaaggt ggccgtggac caattagact
    11281 catatcgcct tttaaaacat tccatagctg tggcaattca tcaatgcttt tggcacggat
    11341 aattttacct attttagtga cacggggatc ttctttcatc ttgaacattg caccttctat
    11401 ttcattttga tcaagtaatt cttccaattt ttcttcagca tctaaacaca tcgaacggaa
    11461 tttatacatt ttgaatgttt ttccattttt accaacacgt gtatggcaaa aaaatatcgg
    11521 acttcctggc tcttcttttc tgattcgata agctacatag aacatcaaag gagaagctaa
    11581 aatcaaacca ataatgcttc ctacaatatc aatcattctt ttaaaaaata aatagatata
    11641 tcgtttttca actttttctt tatccaacca aacttgatta ggatgtactt cactaatacg
    11701 agataataaa tcttcacttt ccatactttc cctctctcct catttccttt ttatctacga
    11761 ataaagaact aaaataaacc aaacttcttc ttttttacct cttcatagac tggataactg
    11821 atttcatccc cgttgatcag atcctttgcg acttgtttca tctgtactac tttttcctta
    11881 ccaaagtcct tagcaatctg ctcatagcac tcttttaagt aaaaggtacg cttagagacc
    11941 ccatgggcat cagaagcagc catctgcaca agattatgtt tgaccatcat tttggctgtt
    12001 ttctggatgt cttttccaaa tacaccgaca taacttggtg cggtgagttg ggccaagcat
    12061 cccatatcta ggaatggcag catatgattc gggtctttac gaaaatgcgc gttgcgttct
    12121 ggatggacga tcactggtgt ctttcctagt tgtaacaact caaaaaagag ctgttctgca
    12181 tataacggca cttccagtgt cggaaattcg attagaagat atgtatcatc taaatctgta
    12241 aataaaattt catctcggtc gatgtcttcg atcagtgttc cggtaatacg tacttcttgt
    12301 ccttccaata acgtcaaagg caattgtctt ttctctaatt ctgcttgtaa cgaagcgact
    12361 agagaaatga cttgtgattt ttcattgctg tattttccat tattatgatg gggtgtgcat
    12421 aaaatatgtg tgatcccttg ttgaatcgct ttttcagcca tggcgatact ggcatctatg
    12481 ttttctgctc cgtcatcgat tcctggcagg atatggcaat gtagatcgat catttctctt
    12541 gtctcctctc tttttatttc ttaattcccg tagtaatagc cagcatcttt gctgtgttct
    12601 gccccattgt atacaacacc aaggactcgc gcttgtacca tatccagcaa ttcttttgct
    12661 ttagtcaatg attctttccg tgacatattt tcacgtacga ccaataaggt gccatccgtt
    12721 ttggaagaca tgatctgtgc atctgttacg gcaactactg gcggcatgtc gaagatcact
    12781 acatcaaata gttgtcttgc ttctgctaaa atctgatcca ttcttggcga acttagtagt
    12841 tctgatggat ttggtggttt aggtccactt ggcagaatcg acaaattctc gactggtgtt
    12901 cgttggatct catctgctac acttcctgat gaactaagaa cggtacttaa tccgcttgcg
    12961 ttgttcaact tgaacgtttt atagatgacg ggcttacgta agtctgcatc gactagcagc
    13021 acccgttgac cagatttggc aaaaacaacg gcaatgtttg ctgcagtggt cgatttccct
    13081 tctccaggtc ctgatgaagt cacaacaatc gttttaattt gctgaccagc tgcggaggca
    13141 aactggatat tcgtccgaat cgtacgatat tgttcagcga ttggtgaaga ggggtttgtg
    13201 agtgtgacca aactgactgc atgagtttgg gctgcttttt gtttctgtgt tcgtgccatt
    13261 tttctttcct cctagactct tgagcggctt ctgcgtgatg gcgctgtttt atcgtttgta
    13321 gcttttggtt gtgtcggagc tggtcctttt ttacggatac cagcattcaa ttctttcgct
    13381 gacatttctg gtacagtccc taagatcggg aagcccaatg tatccgtcac atatttttca
    13441 tcttttactg tccggtcgag taattcaaat aagaaagcta gacctacgcc taccatcatt
    13501 cctaacacta agccaatcac tagattcaat ttattatttg gcgaaacagg tgtcgtattg
    13561 gcaactgcac ctgagatgat cgagatttta tcgacattca tcacgtcttt ggcgttctct
    13621 tggaatactt gtgacgttgt attagcgatt tgttgagccg tcactgcatt tgtgcttgtt
    13681 gcttggatcg agaacatttg tgaattttgt gactgattga cggagatggc atctttgatt
    13741 tgtccagcag tcatttttac attgtcttct gtttctaaac gatctttgac ttcatttagt
    13801 actagatcac tgacgatcat atccttatac gtattgatca tttgcaggtt cgtattgaca
    13861 tcattgacat ttgcagtatc tgattgcggc agtgtcacga tcaattgggc ttgtgagctg
    13921 tattttggcg tcaagatgaa aaacgtcacg atgcctgaga tggccaagcc agcaaacatt
    13981 gagatgagga ttgttatcac gtgtttgcgt aatgtttcaa aaatttcttt caagctgatt
    14041 gtctcttcca ttttttctcc actttcctat ttattctcta gattcatttg atcttttaat
    14101 tcttttttca cacgatcaag ttcttgatcg ctgacttttt ggtatgagat attgtcttgc
    14161 atgaagcctt ctccttgcag ctgatcttgc ttgatcgtac caaatgcatc ccgataatct
    14221 aaagcaatct ttttcatttc atcaaagctc atgtctgttt tcatattcga ctctagagca
    14281 ttcaaaacgg attgatagtt gcttacaccg ttcagactca acactttttt cgctacacct
    14341 tctacgatct ttctttgacg ttcttgtctg ccgtagtcac catttggatc ttcataacgc
    14401 atacgagaat aagacaatgc ctgatccccg ttcaatgtga ttttaccgat cgtaaaatca
    14461 tagccatctt gtgagaaggt gaggtcatta ttgacttcga taccaccaac tgcatcaacg
    14521 agttctttta gacctttcat attgattgaa acatagtgat cgattgggat gtctacatat
    14581 ttttgtacgg tatccatcgc catggcagct ccgccgaatg cgtaagcgtg gttgatcttg
    14641 tcttttgtgt catgtccaac gatgtcgaca tacgtatcac gaggaatgct gacaatcgtt
    14701 gtctgtttgt cttcaggact gactgtggca accatcatcg tatctgatct tccttgatcg
    14761 acccgaccta aatctcccgt atcaatcccc atcaatagaa cagagaaaga ttcttgtttg
    14821 tccagatcaa ctggttttgt tcgttctcct tgggttcgtt ccactgattc gtaagttttt
    14881 tcaatagaat gtgagagatc tagatataat tttgctccta ctcctacgat tgctaatact
    14941 aatacgacaa gaattgctaa aataattaga aatatctttt tccctcttga tcttcttctt
    15001 cgttttgcca tagtttcctc cgtttgctag ccttgcttga attctcgctt attaatagct
    15061 cgtattgcta tagcccattt gctgagcgac tgtttctgct tttgacgcgt atgcggctct
    15121 tattttttca ttccggatct gttcgatgag actgagtgcg gtcaaatatt gatcgtaagt
    15181 agcagtagct gttacttgac cgtctttcag catcgtatcg aatagctgat ctagttgcgt
    15241 gacttgcgcg atctgagtat cagcgaatcc tagatattgc ataacaaccg ttttccaagg
    15301 actatcttta aaaaagctca tattttcacg tacacgagcg acatctgcac ttgtcacctt
    15361 ttctttgatg acgacatcgt ctacagcttg ctgccagttt gatacatttt tagtaaataa
    15421 atcgttgaca gctgtctgga tcttcagttt tgattctgca tcctctaact gcgttaagac
    15481 tgctttcttt tcttcttgga tcgcagcagc ttctttaggt aattctgatt ccttgatgtc
    15541 ataatcatct gccgaaagtt tgatagaaga tagtttatgt gacaaatttg aaactgtttc
    15601 ttctgttatt tcaggattta ggaaaatttt ttcttttgta ctgtacagct tatctacttc
    15661 ttttgataaa gaggtcacgg tgtctttttg attggtgatg gcagattctg cgtaattgat
    15721 ccgttgatga tgttgccgct gattattgaa atggtatcca aacaagcaaa tcacaatagc
    15781 gcctgtgcct accgccacag tagccaataa cttgacgaac ggcttttcca tagattcact
    15841 cctaaatatt gatgatagat aaataaagat gtgattcatt cccatcagaa tgagcgcttt
    15901 ttattttaaa agcataatta aactaatata ttcatagaaa tatttataaa caggttttcc
    15961 ctatataatc ataccacaaa aagttttctt gactagg
//
LOCUS       DAZKCN010000059        15822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000059 DAZKCN010000000
VERSION     DAZKCN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.060"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..388)
                     /locus_tag="ACG8K3_001769"
                     /pseudo
     CDS             complement(275..388)
                     /locus_tag="ACG8K3_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(771..2003)
                     /locus_tag="ACG8K3_001770"
     CDS             complement(771..2003)
                     /locus_tag="ACG8K3_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9145640.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2258..2827
                     /locus_tag="ACG8K3_001771"
     CDS             2258..2827
                     /locus_tag="ACG8K3_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9145641.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3005..3445)
                     /locus_tag="ACG8K3_001772"
     CDS             complement(3005..3445)
                     /locus_tag="ACG8K3_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9145642.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3603..4367
                     /gene="map"
                     /locus_tag="ACG8K3_001773"
     CDS             3603..4367
                     /gene="map"
                     /locus_tag="ACG8K3_001773"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9145643.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4399..5322
                     /locus_tag="ACG8K3_001774"
     CDS             4399..5322
                     /locus_tag="ACG8K3_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP9145644.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5398..6537
                     /locus_tag="ACG8K3_001775"
     CDS             5398..6537
                     /locus_tag="ACG8K3_001775"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP9145645.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6530..7330
                     /locus_tag="ACG8K3_001776"
     CDS             6530..7330
                     /locus_tag="ACG8K3_001776"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9145646.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7330..8157
                     /locus_tag="ACG8K3_001777"
     CDS             7330..8157
                     /locus_tag="ACG8K3_001777"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9145647.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8162..8869
                     /locus_tag="ACG8K3_001778"
     CDS             8162..8869
                     /locus_tag="ACG8K3_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9145648.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8969..9835
                     /gene="rfbA"
                     /locus_tag="ACG8K3_001779"
     CDS             8969..9835
                     /gene="rfbA"
                     /locus_tag="ACG8K3_001779"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9145649.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9849..10421
                     /gene="rfbC"
                     /locus_tag="ACG8K3_001780"
     CDS             9849..10421
                     /gene="rfbC"
                     /locus_tag="ACG8K3_001780"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9145650.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10443..11471
                     /gene="rfbB"
                     /locus_tag="ACG8K3_001781"
     CDS             10443..11471
                     /gene="rfbB"
                     /locus_tag="ACG8K3_001781"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9145651.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11569..12420
                     /gene="rfbD"
                     /locus_tag="ACG8K3_001782"
     CDS             11569..12420
                     /gene="rfbD"
                     /locus_tag="ACG8K3_001782"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9145652.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12454..14487
                     /locus_tag="ACG8K3_001783"
     CDS             12454..14487
                     /locus_tag="ACG8K3_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145653.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14531..15811
                     /locus_tag="ACG8K3_001784"
     CDS             14531..15811
                     /locus_tag="ACG8K3_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP9145654.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4861 a         2729 c         3280 g         4952 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaaacatgtt acaatgaaat taatcaatgg
     2221 tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac
     2281 tagagaacag attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt
     2341 tactgcgaga aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt
     2401 taaaaatatg gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata
     2461 tgaagtgttc actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat
     2521 ccattttgca agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg
     2581 aggaaaagag atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc
     2641 agcttatgtg aatctttcag aagagcagat cgaatctttg tataatggga catggatcat
     2701 agctactggg atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat
     2761 tgaaaagatg atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat
     2821 cgattgaata tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa
     2881 ggaaaagttc gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga
     2941 atgcaatgaa taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag
     3001 tatattaaat ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt
     3061 cttcagcatt cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa
     3121 aagcagcatc gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc
     3181 cgcagacacc gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta
     3241 cgatctcatc aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg
     3301 cctcctcgaa ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca
     3361 gattttcaaa tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag
     3421 catatacgat ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta
     3481 tgaattatac caaaacatca ttgaaaagaa aataaaacct agagacacct ggggaatgtc
     3541 atttggctaa atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa
     3601 atatgattac tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat
     3661 tagcagatgt acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata
     3721 ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct
     3781 ttgaagggta tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc
     3841 ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga
     3901 aaggcggcat ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg
     3961 accgattgat ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag
     4021 ggaatcggat tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag
     4081 cgatcgttcg tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag
     4141 ttcctcatta cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga
     4201 tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg
     4261 cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag
     4321 atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa
     4381 taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg
     4441 cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc
     4501 gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt
     4561 ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct
     4621 gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga
     4681 ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct
     4741 ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc
     4801 cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc
     4861 gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg
     4921 aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc
     4981 atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt
     5041 ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat
     5101 gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc
     5161 atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt
     5221 gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg
     5281 ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg
     5341 taaatttata tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg
     5401 cttgtttctt ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta
     5461 acacctgtat tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga
     5521 agaataaata aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt
     5581 atatcgactt tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg
     5641 attgcttctt tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct
     5701 aaacaaaaaa gtatagggat cttgattgct tctcttatta tttattttgt agcagatata
     5761 cgtattgatt ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt
     5821 cccgtaacga ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg
     5881 gatgggctgg cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat
     5941 tttttcctcc atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt
     6001 attatcggtt tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga
     6061 gctctattcc tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact
     6121 tttgtttcat cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt
     6181 gctatcatta ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt
     6241 catcaccgac taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg
     6301 atggctttga tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg
     6361 atcatattga ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg
     6421 atcaatgaga aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac
     6481 cgtacgcaag taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa
     6541 attaaaccag ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac
     6601 actagataat tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga
     6661 caacaattca cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat
     6721 cgatatctat tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat
     6781 agctagcgat tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac
     6841 agttacagca ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa
     6901 gattcttaat gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga
     6961 ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc
     7021 tgtgtttgaa tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg
     7081 tagttttatg gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt
     7141 cttcatgtac tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt
     7201 attgtacaca cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc
     7261 aaaactgttt cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg
     7321 ttttttctga tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt
     7381 caatctccag cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt
     7441 atttacgata atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt
     7501 tatcatcatg atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa
     7561 gcagatgaag gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat
     7621 tattttgaac tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg
     7681 attttttctg ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca
     7741 gctcaagcag tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt
     7801 gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta
     7861 gattttttgg atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc
     7921 cttcctgtac gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat
     7981 cgataccgtt ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat
     8041 ttatccagtc accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa
     8101 aaccggcata tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt
     8161 cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga
     8221 tagtatttta tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt
     8281 agatgataca tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt
     8341 ttcggggcct cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg
     8401 agatctaatc tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat
     8461 cactgactat tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt
     8521 ggacagcgag ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg
     8581 atggcttaat aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc
     8641 tttaaaaccc gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg
     8701 tttgctggga ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca
     8761 tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg
     8821 tgttcatctg attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt
     8881 taaatcctta atatttccat gacaaatata acaattgatg agataataac aaagtgaaat
     8941 ttgaaatcaa aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg
     9001 aacacgtctg taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa
     9061 accaatgatt tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat
     9121 tatttcaacg ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt
     9181 aggactgcag atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat
     9241 cattggagaa gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta
     9301 ctatggtggc ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac
     9361 agtatttggc tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa
     9421 tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac
     9481 tggcctttat ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga
     9541 acgtggagaa ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc
     9601 cgtagaactt atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct
     9661 tgaagcatct acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt
     9721 ggaagaaatt gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca
     9781 gccattgaaa aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt
     9841 ccttacagat gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg
     9901 tctttggcga tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac
     9961 atggattgaa atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc
    10021 ttcgggggct tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca
    10081 ctggagcagt tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat
    10141 gggacggatt tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg
    10201 cccatggatt cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact
    10261 ataatgcaga agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc
    10321 caattgatcc atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag
    10381 aatttgaagc agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc
    10441 ttatgaaaaa catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt
    10501 acgtggtaaa caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag
    10561 gaaacaaaga gaacttggct ggacttccta gcgatcgcgt agaattggtc gtaggagatg
    10621 ttgcagatgc agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg
    10681 cggcagaatc ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata
    10741 tcattggtac gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg
    10801 tatctactga cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg
    10861 aaggcgaagg agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt
    10921 ctacaaaagc tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg
    10981 caaccatttc aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc
    11041 ctcgccaagt aacgaatatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga
    11101 atgttcgtga ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga
    11161 aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg
    11221 tgatggaatt gatcttagaa atgatgggtc aaccaaaaga tgcgtacgac catgtaaacg
    11281 accgtgcagg acacgacttg cgttatgcaa tcgattcgac aaaattacgg gaagagttag
    11341 gatggaagcc tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg
    11401 aagaacatga agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa
    11461 atggacaata atacagagaa aatgtcactg tattaatgga aaaacacatg tgaggcaggc
    11521 gctcgcctgt ctctttttta aaataaaaat caaggagcgg ataatcatat gtttttatta
    11581 actggaggaa atgggcaact aggtacagaa ttgcgccatt tattagatga aaaaaatctg
    11641 caatatgttt caacagatgc aaaagacttg gatatcacag atgccgagaa aacaatggct
    11701 tacatcacag aattaaaacc tgatgttatt ttccattgtg cagcttacac agcagtagat
    11761 aaagcagaag aagaagcaaa agaattagat gagaagatca atgttgatgg tacaagaaat
    11821 gtagcacttg cagcaaaagc tgtgaatgct caattcgtct atatcagtac agactatgtt
    11881 ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat
    11941 gaatatggtc gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat
    12001 tatatcatac gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg
    12061 caacgcctag cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca
    12121 acttggacac gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt
    12181 ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt atgaattcgc aaaagaaata
    12241 ttgaaagata cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca
    12301 acacgtccgc agtattcagt catgagcctg aaaaagacag aagagttagg atttgtcata
    12361 ccaacttggc aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa
    12421 tttcctttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga
    12481 cgaaaagaaa agccaaaagc gaaccaggca gattcgaatt tggtgcttga cctgtttcta
    12541 aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac
    12601 ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact
    12661 gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct
    12721 ttttacctct cacaagctga agaggacttt ccgttggtga acgaggatta ttcattaaac
    12781 ggacaaaaca tgatcattgc gtataattct cctgcaaagg atattacggt tatcggaaaa
    12841 ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg
    12901 atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagaga
    12961 aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg
    13021 tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat gactgctttt
    13081 tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg
    13141 gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg
    13201 tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg
    13261 gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt
    13321 catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga
    13381 aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat
    13441 tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat
    13501 cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc
    13561 gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt
    13621 accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta
    13681 agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat
    13741 atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca
    13801 tggtcttatt tctatgcgat tacgcattcg aaaatagata attatttttc ggattttgaa
    13861 accatcgcag ttgctgtgtt gggaacagtg attttagcat ttgcattatt tggctggaga
    13921 cgcttgtttt acctcgcttt tgccgcctta gttatcgtat ctggcttttt cgtcaatcca
    13981 gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc
    14041 gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca
    14101 acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag
    14161 gaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct
    14221 tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg
    14281 ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa acgaccatta
    14341 actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac
    14401 ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact
    14461 caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt
    14521 agaaaggaga gtggagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt
    14581 cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag ggttaggttt
    14641 tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat
    14701 tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc
    14761 gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg
    14821 agatttactg tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac
    14881 aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata
    14941 tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc
    15001 gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt
    15061 attgatcagt ggatttgcca gttcttttga acgatggtat gtacgaatca tcggtgcagt
    15121 agcagttgtt gcagcttttc tcgggatttg ggtctgccaa tctagaggtg cattagtcgc
    15181 gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc
    15241 gccatttatt gcttacacgg ttacgattct tttagctttg ccgatttctt atctggcggc
    15301 tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat atttggcata aattctatca
    15361 gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccaacg
    15421 aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata tctatggatt
    15481 gataggattt gggattgcta ctttgctgct tatccttttt attggtcgct taacgttgaa
    15541 agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca
    15601 gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt
    15661 ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct atgttatgga
    15721 agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc
    15781 tcattcttcc aggatccaac gctatcattg ataggcacaa aa
//
LOCUS       DAZKCN010000060        15465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000060 DAZKCN010000000
VERSION     DAZKCN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.061"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(362..1480)
                     /locus_tag="ACG8K3_001785"
     CDS             complement(362..1480)
                     /locus_tag="ACG8K3_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145655.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1713..1835
                     /locus_tag="ACG8K3_001786"
     CDS             1713..1835
                     /locus_tag="ACG8K3_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145656.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1951..3258
                     /locus_tag="ACG8K3_001787"
     CDS             1951..3258
                     /locus_tag="ACG8K3_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP9145657.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="ACG8K3_001788"
     CDS             3377..4576
                     /locus_tag="ACG8K3_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP9145658.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="ACG8K3_001789"
     CDS             complement(4603..5871)
                     /locus_tag="ACG8K3_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP9145659.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5926..5992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6034..6680)
                     /locus_tag="ACG8K3_001790"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="ACG8K3_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="ACG8K3_001791"
     CDS             complement(7088..7591)
                     /locus_tag="ACG8K3_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9145660.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="ACG8K3_001792"
     CDS             complement(7714..8478)
                     /locus_tag="ACG8K3_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP9145661.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8585..8860
                     /locus_tag="ACG8K3_001793"
     CDS             8585..8860
                     /locus_tag="ACG8K3_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9145662.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9576..10529
                     /gene="yidC"
                     /locus_tag="ACG8K3_001794"
     CDS             9576..10529
                     /gene="yidC"
                     /locus_tag="ACG8K3_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9145663.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10651..11860
                     /locus_tag="ACG8K3_001795"
                     /pseudo
     CDS             10651..11860
                     /locus_tag="ACG8K3_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(11905..12305)
                     /locus_tag="ACG8K3_001796"
                     /pseudo
     CDS             complement(11905..12305)
                     /locus_tag="ACG8K3_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723846.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12552..12986)
                     /gene="tnpA"
                     /locus_tag="ACG8K3_001797"
                     /pseudo
     CDS             complement(12552..12986)
                     /gene="tnpA"
                     /locus_tag="ACG8K3_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13164..13530)
                     /locus_tag="ACG8K3_001798"
                     /pseudo
     CDS             complement(13164..13530)
                     /locus_tag="ACG8K3_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(13544..13829)
                     /locus_tag="ACG8K3_001799"
                     /pseudo
     CDS             complement(13544..13829)
                     /locus_tag="ACG8K3_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13838..14029)
                     /locus_tag="ACG8K3_001800"
                     /pseudo
     CDS             complement(13838..14029)
                     /locus_tag="ACG8K3_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14029..14505)
                     /locus_tag="ACG8K3_001801"
                     /pseudo
     CDS             complement(14029..14505)
                     /locus_tag="ACG8K3_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14973..15326)
                     /locus_tag="ACG8K3_001802"
     CDS             complement(14973..15326)
                     /locus_tag="ACG8K3_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145664.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
BASE COUNT         4555 a         3264 c         2783 g         4863 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcctggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 tgcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagtaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttacctgtta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 cagacatggg ctgagccgct tctttgactg cgcagtattt gtccacgaat gtcacaatat
     7201 atccttcttt atacttaggc ggtgcgatcg ttgcacccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaaa tcggtgattt tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtcccc tcgtcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttgtaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tttgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgctct
     7681 agataaaata gctaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacgat
     7741 ccctgctgcg actccgacat tcaatgattc cgcatttcct ttcattggga tatatacgtt
     7801 ctgatcagtc gaagacaaga tttcctgact tactccctga ccttcgtttc ccatgattat
     7861 agcgaaatcc tctgtttttt ctactttgtc taatgcatgt gcttcttcat ttaattctgt
     7921 accgtacacc gtaaaccctt tgcctttcat tacagggatc acttctgcca aatctttttg
     7981 taacacaggc aaatgataat tgcttccctg catgcttcgc agcacctttg tactataaat
     8041 atctgccgtt cctttaccta ataaaacacc agccatacca aacgcatctg ccgtacggat
     8101 catcgttccc acatttccag gatcctgtac attatctagt aaaagccagc ctcctgaaaa
     8161 atctggcgta tcttcagatg ttaacgtcat cactgctaag atcccttgcg gtgtaggtag
     8221 atctgacata gcggtcatga cctcatctga aactagaaaa gtagaaatct ctttgtttac
     8281 agccagccag tctccccatt ctttcatacc tctctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcgatcag atggaaccct tccaaaagat attctgcctt
     8401 ttcttctctg tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttac
     8461 tgattggatc tctttcacat tcctcacctc aactgattat atcatggaat aggtgcttga
     8521 gcttgtttca tcctaaaaaa atcggtatca ttgatttata aaacaggaaa gaaaggtgac
     8581 gaacatgcgc aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg gatttcgcta
     8641 tatgaccaag atggttgctg atcagttagg cgtgaccgga acggtaaaaa acgaagacga
     8701 cggctcagtc acaatcgaag caatcggaaa tgacgacatc atccaaaaat ttatcgaaga
     8761 agtcaagaag tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagatcc
     8821 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgttttt gaagcgcttt
     8881 gcctcgagta ttaaggagaa acattttcca aatagcttct catattttga aatgtaatga
     8941 cttaataccg agaggcagct gataaaacac cgtcaataag gttgtgaaaa agctggtctg
     9001 catcaagcat taaggactgg ttgtgaaaaa tagctctcca tatttttcaa caggctggac
     9061 ttaatgccgt agatgcaagc ttttgaacat cgtcgataag gttgcgaaaa agctgatctg
     9121 catcaagcat taaggactgg ttgtgaaaaa gtcgcttagt tttgagtatt aaggaaaatt
     9181 ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccaag aaactgactt
     9241 ttgaacatcg tcgataaggt tgtttttgag gcactttgcc tcaagtatta aagacaggta
     9301 gtaaaaaagc tgatctataa cttgcaataa atttgcttaa cccatatttt ccagacagaa
     9361 caaaaaagtt tttagttttt taaataagaa agaaacactt ggaatcactc ttttctttct
     9421 aagtatttct ttcttgattt tttgtctttt ttcaaaattt ttcgctacga aaaagaacga
     9481 atatttatat gaaatttcta tgaagttgat tgaattccat gcgctttttt agtatagtta
     9541 agtttgtgta aaaattatag aagaggaagt attctatgaa taaatggaaa aactggttat
     9601 tgggatccgg acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg atggattcaa
     9661 acggaaaccc tgacacttca ggaatcgttt atcgagtact cgtccatcct atgggccaag
     9721 cgattacata tctagttgag aatttcaatt ggtcttacgg ctgggcagtt attgcaatga
     9781 ctgtgatcgt tcgtatcatc atcttgccac taggtatcag tcaatcgaaa aaaacaatga
     9841 tccaatctga aaaaatgcaa gcgttaaaac cacaagttga agcagcacaa caaaagctaa
     9901 aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag caagtatatc
     9961 gcgaaaatgg attgagtatg accggcggaa tcggctgtct gcctctattg atccaaatgc
    10021 cgatcttctc tgccttgtat tttacggctc gctacacaga aggtatccgt gaatcatctt
    10081 tttacggcat cgatctaggc tcaccaagta tggtgcttgt ggccatcgca gggggcgctt
    10141 atctgcttca aggatatatt tcaacaatcg gtattcctga agaacagaaa aagacgatgc
    10201 gtacgatgct gatcgtctct cctgctatga tcgtcttcat gtcgatcagt gcgccagccg
    10261 gtgttacact ttactgggta gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa
    10321 acgttataat gaaaccgcgt ttgaaagcac aagtggcaga agagttgaaa caaaacccgc
    10381 caaaacaagt cgttactcca agaaaagacg tgacagaatc agcagcaaag ccagctgaca
    10441 acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac aaaggccgca
    10501 acgcaggaag gcagcagaag cggaaataaa aatatatgac aaaaaactgt agacaaataa
    10561 aaaaatttct ttgttctaca gtttttttgt tacaattaaa gaaaacgttc gcatttcgag
    10621 gaggaatgta taatgatgtc caaataatca atgtatagtc gaatggaaga agacaaaatt
    10681 tcgctagatg cacttgttcc taaaaatcat ttagtccgta aaattgacaa attattgtca
    10741 tttgatttta tttatctcat tgttgaatca acttactcga ccattggtcg ccctagcgtt
    10801 gatcctgtcg tcttagtaaa attagtattc attcaatatt tatttggaat ttgttcgatg
    10861 cgtcagacca ttaaataagt tgaaacaaac ctagcttatc gttggtttct tgggcttagt
    10921 ttaattgata aagttcctca tttttcaact tttggtaaaa actatgcacg gcgtttcaga
    10981 gaaaccgatt tgtttgaaca gatatttatc attattctcg aataagcagt tgatgctaga
    11041 tttattcatg aagaaatttt gtatatggat tcaactcata ttaaagcgaa tgcgaacaaa
    11101 cgtattttta caagagatat ggcacataaa gaagccattt tataccaaaa acaattagaa
    11161 gaagaaatca atgaggcaag aattgccgaa ggcaaacgtc cttttggttc aatcattcgt
    11221 caggaggtgg tcgaaagaaa atttagcatc gcagatccag aaagtggtta ttatgtaaaa
    11281 acagaacgag aaaaacagtt tgcttattct gctcataccg ttggtgatga aaatggtttt
    11341 gtattagatg tcatgattac gcctggaaat ctacatgata gccgaatgct cgtgccacaa
    11401 atcgaacgcg ttaaatcacg ttatggtgta gctgctaatg ctgcgtacaa aacaccttgg
    11461 aacgtcaagt atttaatcga acgaaaacta cgacccattc tcccttatat cagtttcgca
    11521 caactactcg agatggctat aaaaaatatg tatctaaatc ttttatttgt gaaagttgtc
    11581 cattattgaa aaattgtaca gaaagccaaa aacaccaaaa agaaatacat cgacatattt
    11641 gggaagacta cttagatgaa gcagaacatt tacgccatac ggtgtacaat cgtgaaaaat
    11701 ataaaaaaag aaaagaaacg attgaacgca tttttgcaga cgcaaaggaa aaacatggaa
    11761 tgcgctacac caagtatcga ggcttggaaa aaatcaggtg catacaatgc ttgtttttgt
    11821 cgcaatgaat ctgaaaaaat tagctacttg gcagtgggag catatgtcgt tatatctttt
    11881 attatacaaa aatagacaaa agtatcagtc tacactctta gtaaagagga aactgatgct
    11941 tttgtctaca gtcttagaga gtgggacaat agttttttct tctttacgtc ttttcctctc
    12001 catgtatttt cctaattttc ttaatttaac gccatgagtg ccaagccaat gtcaatcttg
    12061 gctccttgtt tccttcataa gtggaaatcc gtttccgata gatcgaacct tctttttcct
    12121 ctaaaagttt ttttacattc tgctttgaaa taacgccatg tattattcac taaaatttgt
    12181 cggtttcgct cactttttgc tttcgtacat tgactacgaa caggtcagca ccgaccactt
    12241 tcacattcgt aaattttgaa gacacgtata aatccacttt tatcttttct tcgactaaag
    12301 cgttgaccgc aaaagcgaca tctgtttcag aaatatattg ttccatatct aatggtaaaa
    12361 ttttttggtt gatggtataa tttttaaaca taaagcactc gcttcctttc tgattgggtt
    12421 tggtcacttt aatcctacag ggatgagtgc tttttgtgta ccttaaaatc agtaaatatc
    12481 catccgtgaa aacgggtggc tttttaacag ccctagaagg gctcaccact cacagcccta
    12541 tctctcttta actatttaaa tggatctaca tactcttgtt tacttatact gtctctaatt
    12601 cgatcatctg cttcttgttc tcaaatatat atttcaattg ttttttgatt taacccaacc
    12661 gtactcacgt agtatccttt ggtccaaaag cttttattcc catacttata tttcaaattt
    12721 gcatgttttt catggattaa ggttgcactt cgtcctttta agtagcccat aaaactagaa
    12781 atactaatct ttggcggtat tcttactaac atatgaatgt gatcaggcat tgcatgagct
    12841 tcaatgattt caacatcttt cctctcacac aattttctta atatctggcc aatacttact
    12901 cttaactttc cataaatcac ttttcgtcga tacttcgggg taaagactat gtgatactta
    12961 caatcccatc tcgtatgtgc taaactttta tcgctaaagc tatttcgtcc acgcgtagaa
    13021 acgcgcgttt ttttgtttcg tacgctacgc caacgaaact gactaaagtc aataaaaaaa
    13081 gtggggcaat aatcagattt ttttctgact attgtcccac tctcttttta ttactcttca
    13141 tcttctacta ttccactaat taattagttg acagatacaa tgctttcaca tcctgcacga
    13201 acaaactcgg gctctgaacc aatcacttgc gagttgataa attcaatgac cataggaaga
    13261 ttaatccgac ataaagttta atctgttgtc cttccattat cagtttagaa atgatattag
    13321 taggtgtacc gcctaaaaga tcgcatagta ccacaaaatc atttaacgaa tctatgacag
    13381 taagagcttt tgtctgtatg tttcagtgcc atcttctggt aataaaggta acgtgtagat
    13441 attctcttga ggtcccataa tcatttttgt actttttttc aattcctcac aaaattttcc
    13501 gtgactaatc aatatcaatt ttctactcat ttttggctct cccttatttc gaaatcacac
    13561 acaatgcaga taaagccaca cacaagtaat acgataaaaa tcgctcgagt tgggtcatat
    13621 ttttctttcc taacaaccaa taaacacctc gacgatccca gcgagtaaga ttttagataa
    13681 tatcatatcg atatagtttt gcatattcaa tgtcacatcg ccaatcgttg gtaccataaa
    13741 aatttaatat caatcattgt tgcaactaat gctcctacca tgaacactct taataaagtc
    13801 gctacgtccg ttaatacagt taagcgagat tacactagat gatctgttcc tgccaaggca
    13861 agagccagaa gcggttgcct gcccatttgc ctttggtgtg tcggagctgt ccgataaagt
    13921 tcaccgcccg atccgctctg gctttgtcgt agtgacattc cgaaagcatg aatcggcttg
    13981 gtgtgtattc ctttaatttg ggatagccct ttggtctggt ttctgccatc agctaccacc
    14041 tcccaacaga gcctccatcg catcttctgc tccgacacca ccgccatcac cggcaatgat
    14101 acggcttctg gacgaaggcg tcagaccgaa ctgctcggaa aatcggttca taattttcag
    14161 ataggtctgg gcaataaaca cctgtggtac ctgctgccaa tagccggata gggttttcac
    14221 gatgataccg tgctgggtaa tgaattcctc ggcttccttc cagcgggcgt aggcttggca
    14281 ctaaccggca aaggcagcca tatccacatc tgtcaagatt ccgatggcct ccatctgctt
    14341 tgccagacgc ttccattcct tctttgcctc tggttccagc cacttcggac aggtcggtgc
    14401 cttcttttcc ggcgttgact cttttgcatt gagtgggcga ttgcccggat ttccctccag
    14461 cttcttcatg gctgtcgggg ttgattttct tcctctggtt gccacagtcg tcacctccct
    14521 tcaatgtatt tctgccagta gacagagaac agagccaagt cgaaacctga ctctcttctc
    14581 tatctgctaa cttgtatttc agtccactct tctgatgaat cgcacccgca ggattttcaa
    14641 gatgccgcag gcaaattcca agaagtttct gaccgcatca taaccatcgt agccgtgtag
    14701 ccacatcctt ccacctcgcc gccgtcctcg tcgctgatat aaatgattct gtattcgctc
    14761 atctgttgtt ttctccagtc ttttgttttt ttgtagtaca tatatcactc taaaagtgat
    14821 tattatcaag tcgtttgaaa gccataatgt acacaatcat tcgactggtt tttggttcat
    14881 aattgaccgc caaagtgagc aaaagaaagg gacctctgtc tgtttcagaa gtccctttcg
    14941 gtatttggtt cttagtggtt caggctgtat tctcaaacct catactcatc ttcgaggtct
    15001 gcctctgcaa tgatctggcc gatgctgtcg taacgctgga cctgcatctg gatggcatcc
    15061 ttgtctgctc cctcgtccat catcttcagc agcttgtctg attccttttt gtaggcccag
    15121 aaaaggatct cctgaaggct ggtctcctgc tttttcttgc gaagggcgac cttcttctct
    15181 tccaccgcct cgttcaactt ggtgaactct gcccaagcaa ggataccgtc tcgcttgatt
    15241 gcgatctcca catccttcag ctccgggatg gtctttgcac ccttgatctc cttcaaatat
    15301 ttctttgcct tttcgctcat tgccattagc gtttcctcct tgtttttggg attttcccct
    15361 ttggttgtgt gtatattgcc atagtctctc gactatatca agtcaatcca caggcatata
    15421 ctacacaatc atttggagct gtatttgtat acattatgcc gattt
//
LOCUS       DAZKCN010000061        15247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000061 DAZKCN010000000
VERSION     DAZKCN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.062"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..251)
                     /locus_tag="ACG8K3_001803"
     CDS             complement(21..251)
                     /locus_tag="ACG8K3_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145665.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="ACG8K3_001804"
     CDS             complement(687..1253)
                     /locus_tag="ACG8K3_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9145666.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="ACG8K3_001805"
     CDS             complement(1253..2209)
                     /locus_tag="ACG8K3_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9145667.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="ACG8K3_001806"
     CDS             complement(2209..2865)
                     /locus_tag="ACG8K3_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9145668.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="ACG8K3_001807"
     CDS             3235..5253
                     /locus_tag="ACG8K3_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9145669.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /gene="leuS"
                     /locus_tag="ACG8K3_001808"
     CDS             5680..8094
                     /gene="leuS"
                     /locus_tag="ACG8K3_001808"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9145670.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="ACG8K3_001809"
     CDS             8442..9995
                     /locus_tag="ACG8K3_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP9145671.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="ACG8K3_001810"
     CDS             complement(10376..12160)
                     /locus_tag="ACG8K3_001810"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145672.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="ACG8K3_001811"
     CDS             complement(12175..13896)
                     /locus_tag="ACG8K3_001811"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145673.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="ACG8K3_001812"
     CDS             13996..14757
                     /locus_tag="ACG8K3_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9145674.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4958 a         2793 c         2997 g         4499 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgctta
//
LOCUS       DAZKCN010000062        14828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000062 DAZKCN010000000
VERSION     DAZKCN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.063"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..451
                     /locus_tag="ACG8K3_001813"
     CDS             <1..451
                     /locus_tag="ACG8K3_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9145675.1"
                     /translation="LYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLS
                     SSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISS
                     ARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            651..965
                     /locus_tag="ACG8K3_001814"
     CDS             651..965
                     /locus_tag="ACG8K3_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9145676.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            986..1363
                     /locus_tag="ACG8K3_001815"
     CDS             986..1363
                     /locus_tag="ACG8K3_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP9145677.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1373..2146
                     /locus_tag="ACG8K3_001816"
     CDS             1373..2146
                     /locus_tag="ACG8K3_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145678.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2168..3571
                     /locus_tag="ACG8K3_001817"
     CDS             2168..3571
                     /locus_tag="ACG8K3_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9145679.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3753..4937
                     /gene="mobT"
                     /locus_tag="ACG8K3_001818"
     CDS             3753..4937
                     /gene="mobT"
                     /locus_tag="ACG8K3_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP9145680.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4934..5224
                     /locus_tag="ACG8K3_001819"
     CDS             4934..5224
                     /locus_tag="ACG8K3_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145681.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5221..5442
                     /locus_tag="ACG8K3_001820"
     CDS             5221..5442
                     /locus_tag="ACG8K3_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145682.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5484..6263
                     /locus_tag="ACG8K3_001821"
     CDS             5484..6263
                     /locus_tag="ACG8K3_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9145683.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6324..6824
                     /locus_tag="ACG8K3_001822"
     CDS             6324..6824
                     /locus_tag="ACG8K3_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9145684.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6880..7518
                     /locus_tag="ACG8K3_001823"
     CDS             6880..7518
                     /locus_tag="ACG8K3_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFP9145685.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7590..7985
                     /locus_tag="ACG8K3_001824"
     CDS             7590..7985
                     /locus_tag="ACG8K3_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9145686.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7969..10422
                     /locus_tag="ACG8K3_001825"
     CDS             7969..10422
                     /locus_tag="ACG8K3_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9145687.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10419..12446
                     /locus_tag="ACG8K3_001826"
     CDS             10419..12446
                     /locus_tag="ACG8K3_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP9145688.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12443..13465
                     /locus_tag="ACG8K3_001827"
     CDS             12443..13465
                     /locus_tag="ACG8K3_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP9145689.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13482..14411
                     /locus_tag="ACG8K3_001828"
     CDS             13482..14411
                     /locus_tag="ACG8K3_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP9145690.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14725..14772
                     /locus_tag="ACG8K3_001829"
                     /pseudo
     CDS             14725..14772
                     /locus_tag="ACG8K3_001829"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5022 a         2479 c         2935 g         4392 t
ORIGIN      
        1 tttatacaaa tttgttacca aacatatatt tgttaatctt aatattgacg agaaaaatta
       61 cccgtctatt tttaagcact atctattttt tattaacgaa gtagaagatc ggataaaaaa
      121 taattcatta tctagtagtg tatctagttt taagaagatg tttaaatctc gagatggtat
      181 cacacttact acagcacata aagttaaagg aaatgagtat gatactgtta tcgctattaa
      241 tcttctaaac gggaaaattc ctcattggaa tgaaattttt aatgtttctg ataaaggaat
      301 tagttcagcg agaaaactac tatacgtaat ttgttcaaga gcaaaaaata atttatttct
      361 tttctcagag gtgggttatt ttacgagtaa aggagacttg ctttatccca ctcaattatt
      421 agataaaata gaatttgatt ataatattta aaatacctag gtagtcgttg ttttaaatat
      481 agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt gaaaggaagt
      541 gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt taaaaccatc
      601 acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat atggaactta
      661 aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct ggtgaaggga
      721 acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt tacaatttat
      781 actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa gctggcgaga
      841 aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc gcagaaggtt
      901 ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat atggtgaaag
      961 cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga ttgaaaaaga
     1021 ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta tccagaatga
     1081 agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat caaaaggaca
     1141 aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat ttgactacaa
     1201 cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta cttttcgagg
     1261 ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac agacaaaacc
     1321 tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc ttatgacaaa
     1381 cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta ttcggcaata
     1441 ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt tttcagaaga
     1501 aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa ccatacgcaa
     1561 actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa agatgtgttt
     1621 tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag ctctaattaa
     1681 gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg gaattgaggg
     1741 aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt tcaagacaag
     1801 tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc gccttttatt
     1861 agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc aggcattttg
     1921 cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct atccgttgac
     1981 aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt atccgaaaga
     2041 aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca tttctcaacc
     2101 aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg tggaaaggag
     2161 gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg ataagcactt
     2221 ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc tactcccttt
     2281 tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata catttttcta
     2341 tcaacttcat acgccatact tttggagcag tattctcact gctctttttg tatgcggact
     2401 tatcagctgg ctttattatc actatcgtat agattctata aaacagttag agcaccgtca
     2461 aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa ctcaatcaga
     2521 gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc attttcctaa
     2581 aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca ccttagggaa
     2641 atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt attgtgaatt
     2701 agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg atactattgg
     2761 caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat tgatggaatc
     2821 ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca ctggtagtgg
     2881 aaaatcctat tttattctca cattgattga atctctcctg catacaaatg caaagcttta
     2941 tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga ataatgtcta
     3001 ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata tgattgatcg
     3061 cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg cttatcttgg
     3121 tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata tgctaggtcg
     3181 tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac gacaagcagg
     3241 cttcttttta attcttgcct gtcaacgtcc agacgcaaaa tattttagtg atgggattcg
     3301 ggacaacttt aacttccgtg tggctttggg tcgtatgtct gaaatgggct ttggtatgat
     3361 gttcggaagc gatacacaaa aacaattttt cttaaaaccc atcaagggaa gaggttacgt
     3421 ggatacagga aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa aaaggtacga
     3481 tttcttagga gaaataggaa aagtcattca aaaaaagcaa tctgagcctg taccgcacga
     3541 agtgaaaggt acaggttcag acttgctttg attggtgtgg cgttagccac gccaatcctt
     3601 tagccccctc tatctaacag gggggcacaa atacaactga aaacaggcaa ttctcatcaa
     3661 ccaatgttga tggtacaaag gattgcggaa aatatgaaag gtgtaaactc aagggtcata
     3721 gtttacatct tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt tggattaagg
     3781 aattgaaaga aaagcgactg gcttatggcg tttctcaaaa taaactggcg gttgcttctc
     3841 atattaccag accttaccta tcggatattg aaacaggcaa ggcagtccct actcaacagg
     3901 tgaaagaaga tttactgaac gctttggaac gcttcaatcc cgataaccca ttggagatgt
     3961 tattcgacta tgtacggatt cgattcccta ccaatgatgt ggctcaaatt atcggtgagg
     4021 ttttacgttt gaatatggat tatatgctac acgaagactt tggctattat tcttatcccg
     4081 aacactatcg tttcggtgat attgttgtcc tagtctctca tgatgtaagt aaaggtgttt
     4141 tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg gctcaacatc
     4201 ggagctggta tgatttcttt caagattgca tggaacataa aggtattttc aaacgcttgg
     4261 atttagctat caacgataaa acagggattt tgaatattcc agatttggca aagaaatgta
     4321 aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt ggtgaattgg
     4381 ttcaccgtga tgaaaaacca tacatgggaa acacacttta tattggctca ttaaaaagtg
     4441 aagtctattt ctgtatatat gagaaagatt atgagcaata cgtgaaaaat gatgttccct
     4501 tagaagacgc agaagtaaag aatcggtttg aaattcggtt aaaaaatgac cgagccaccc
     4561 aagccatgaa agatttattg gcacatcaac aggcggaaaa aacagccttt gaaattatca
     4621 atcgctatat ccgctttgca gataaagacg acactaaacg tcggagtgat tggaaaacta
     4681 atgaacgttg ggaatggttt atcggtaaaa atcgtggagc attacgccta accacacagc
     4741 cagagcctta ttcttttgaa cgaacattga actggttaca tcatcaagtc gcccctacat
     4801 taaaaatcgc aagcatttta gatgttctca acggtacaac cattatctcc accatgattc
     4861 aggaagccaa actgacagaa aaacatgaga aattaattga acaacaacat ctagccatgg
     4921 aggatttgat tacatgagcc ttttaacaaa agaattaaag aagttagggt ttcagtgtgg
     4981 cattgagttt caagcgtata tacaaaatac agggaaatat acgtctctca ttattgaggg
     5041 aaaacggcaa gctggtgata ccatctatac ctatgatttt tacaaggtga gattttataa
     5101 taattatacc aatcgagtaa ccgtctatgg agagcattta accccttttc aattattaag
     5161 gcgtgtaaaa agctatatct attatcgaga aaaatatctt aaggaaagga ggacaatcac
     5221 atgaattttg ggcagaattt gtacaactgg ttcttggata atgcacagtc attggtattg
     5281 ttagcgattg ttgtcattgg actgttttta ggttttaaac gagaattttc aaaactcatt
     5341 ggcttcttga ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc tggcgtgaaa
     5401 gatgtgttat taaacttatt taaccgaatc attggtgctt aacagatatt tttcatgata
     5461 aaataggaag tatatatttt gttatgaaag gatttatgaa tatgtttaca aatgatcaat
     5521 ttaaatctct tttaattaaa acattagaaa gaaagagctc ttcaaattat tatgaaggag
     5581 gtaacgagat tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt
     5641 ttacttatta taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa
     5701 gagttccttt tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag
     5761 caagctccat atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac
     5821 ttccagagaa ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac
     5881 aagctattga agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca
     5941 gagttcacac tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag
     6001 ttcctagaga cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag
     6061 aaattcaacc tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa
     6121 tgattagctc tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata
     6181 ttattggaaa gagagaagca aaactaataa taggtatttc aagtacagtt acagattata
     6241 ttagcggata ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt
     6301 ttataaaaag gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt
     6361 ataatgaagg tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg
     6421 cagaaagaat cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc
     6481 cttttgatgt ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc
     6541 aagaaatcga aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta
     6601 gtagcttgga agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt
     6661 cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt
     6721 ccaatttaca aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta
     6781 attacctagt tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt
     6841 tataattaaa ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat
     6901 cttaaactat taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt
     6961 cgttacaaac agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt
     7021 ccagaatatg ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta
     7081 gctgtctatg ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt
     7141 ggtatcgaat acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct
     7201 cgatttattc cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt
     7261 ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga
     7321 agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa
     7381 acgagattgt atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc
     7441 agatattcaa tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca
     7501 aaaaataaaa ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt
     7561 ttctattttt acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt
     7621 ggtcagtaga aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct
     7681 ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac
     7741 caccactttc tctgattgac ggagcactac tcaaatactt agggattccc attgttctca
     7801 ctgggttcat gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta
     7861 tcattaccta tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa
     7921 aagaaacaat gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg
     7981 attaaatata tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat
     8041 gaattaattc cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat
     8101 aatttctgtc aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca
     8161 accgaatcaa gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta
     8221 aaagagatag cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt
     8281 gacaatcaag tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa
     8341 gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt
     8401 gtgaatcaca aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt
     8461 tctaagatgg aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg
     8521 gagaaagatg attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt
     8581 gatgactacg actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa
     8641 tacgatattc tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt
     8701 gaacaggaag ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa
     8761 ttggatttcc ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt
     8821 gataccagta tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat
     8881 aaaaagaaag aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt
     8941 actaatgtgg tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc
     9001 aaagaatcta tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag
     9061 ctgaaacaac gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta
     9121 cgaccttttg gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat
     9181 atcaatgact atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct
     9241 acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga
     9301 aatatttatc tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca
     9361 ttagcgtccg cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg
     9421 gtctattatt cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt
     9481 ggtgaatgga aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg
     9541 agtgagaacg acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat
     9601 tcagaaagcc ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc
     9661 gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg
     9721 ttgttattgg tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct
     9781 gaccatatcg aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt
     9841 gtcacccaat ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc
     9901 ttacccgatt ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca
     9961 atgctgattg tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt
    10021 aaaattgttg accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta
    10081 tctatgcgat tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa
    10141 aacgcggacg atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt
    10201 cgttccactg acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa
    10261 gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac
    10321 atctatggtc gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc
    10381 tttgatacca gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat
    10441 ttcttcacta tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca
    10501 cgctgggcaa tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg
    10561 aatactccaa gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg
    10621 gctggcttcc atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg
    10681 ccattactaa ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg
    10741 tgaaagaagc ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca
    10801 tgcagacgat tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt
    10861 tcctccttct ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc
    10921 gagaaacgac gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg
    10981 cttcctttat tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg
    11041 tcagtaccgc cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag
    11101 ggaaagacag tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat
    11161 ggctattgct tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa
    11221 gtttattgtc taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg
    11281 aagaaattga agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag
    11341 gaacagtctt ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa
    11401 caggaatcat gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca
    11461 gtttcctatt aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt
    11521 tatttaatgt gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta
    11581 tttccactat gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac
    11641 aaattgtgac ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt
    11701 tacaaagcaa cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga
    11761 tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag
    11821 ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata
    11881 ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc
    11941 aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa
    12001 atcgtctagt caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg
    12061 tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac
    12121 aatctatggc agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa
    12181 aacgaaagaa cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa
    12241 aagaaaaccc atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa
    12301 ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa
    12361 aagagcaaaa acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa
    12421 cggataagcg aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt
    12481 ctctctcttc ttttttctct tattattcct agtcattttc tttgcagatg atacggacag
    12541 tggcgaaaac aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt
    12601 gttggcacac cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt
    12661 ttcttatatc ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca
    12721 aagttccgaa tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca
    12781 aggggttaaa tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga
    12841 ttctgttatc caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg
    12901 gaaaaaatat acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa
    12961 agcagactat cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg
    13021 aaatcagttt tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt
    13081 tgatgatgaa accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta
    13141 tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt
    13201 gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca
    13261 agaaatctct atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa
    13321 tgcaggcaca tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc
    13381 aggcgaccca atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg
    13441 ggctagacgt gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc
    13501 gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc
    13561 cgatgattaa agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg
    13621 gtagtgtcgg atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg
    13681 aaacagaaat cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat
    13741 cctttgcgaa agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg
    13801 aaaaattgac ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta
    13861 aggatattcc tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga
    13921 atgagaacac ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca
    13981 aggaaactat atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg
    14041 ttattatcaa aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac
    14101 agctagaaag tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag
    14161 aaacgttctt ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta
    14221 atcatgtgtt acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct
    14281 ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa
    14341 caaaggcaac ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga
    14401 ttgtaaaata acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt
    14461 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta
    14521 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    14581 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgca cccagtttaa
    14641 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    14701 atggttatgc ataaaaatcc cagtgataag agtatttatc actgggattt ttatgccctt
    14761 ttgggctttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    14821 gttgatgc
//
LOCUS       DAZKCN010000063        14352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000063 DAZKCN010000000
VERSION     DAZKCN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.064"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ACG8K3_001830"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ACG8K3_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9145691.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ACG8K3_001831"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ACG8K3_001831"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9145692.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ACG8K3_001832"
     CDS             3349..3552
                     /locus_tag="ACG8K3_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9145693.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="ACG8K3_001833"
     CDS             complement(3653..4315)
                     /locus_tag="ACG8K3_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145694.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="ACG8K3_001834"
     CDS             complement(4328..5830)
                     /locus_tag="ACG8K3_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145695.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="ACG8K3_001835"
     CDS             6378..8057
                     /locus_tag="ACG8K3_001835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9145696.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="ACG8K3_001836"
     CDS             8184..8663
                     /locus_tag="ACG8K3_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP9145697.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="ACG8K3_001837"
     CDS             8882..9562
                     /locus_tag="ACG8K3_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9145698.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGDPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="ACG8K3_001838"
     CDS             9570..10901
                     /locus_tag="ACG8K3_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP9145699.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="ACG8K3_001839"
     CDS             10916..11878
                     /locus_tag="ACG8K3_001839"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP9145700.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="ACG8K3_001840"
     CDS             11989..12327
                     /locus_tag="ACG8K3_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9145701.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="ACG8K3_001841"
     CDS             12406..13113
                     /locus_tag="ACG8K3_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP9145702.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13216..>14352)
                     /locus_tag="ACG8K3_001842"
     CDS             complement(13216..>14352)
                     /locus_tag="ACG8K3_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145703.1"
                     /translation="EQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSI
                     RLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNK
                     QQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPN
                     QISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVF
                     FELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLI
                     QHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQH
                     EKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4577 a         2491 c         3099 g         4185 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gatcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
    13201 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    13261 ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    13321 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    13381 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    13441 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    13501 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    13561 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    13621 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    13681 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    13741 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    13801 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    13861 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    13921 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    13981 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    14041 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    14101 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    14161 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    14221 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    14281 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    14341 ataaagttgt tc
//
LOCUS       DAZKCN010000064        13709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000064 DAZKCN010000000
VERSION     DAZKCN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.065"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..346)
                     /locus_tag="ACG8K3_001843"
     CDS             complement(26..346)
                     /locus_tag="ACG8K3_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9145704.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECFFIYDI
                     IASK"
     gene            647..2527
                     /locus_tag="ACG8K3_001844"
     CDS             647..2527
                     /locus_tag="ACG8K3_001844"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9145705.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2606..2833
                     /locus_tag="ACG8K3_001845"
     CDS             2606..2833
                     /locus_tag="ACG8K3_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9145706.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2838..3380
                     /locus_tag="ACG8K3_001846"
     CDS             2838..3380
                     /locus_tag="ACG8K3_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9145707.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3631..4308
                     /locus_tag="ACG8K3_001847"
     CDS             3631..4308
                     /locus_tag="ACG8K3_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP9145708.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4650..5870
                     /gene="sstT"
                     /locus_tag="ACG8K3_001848"
     CDS             4650..5870
                     /gene="sstT"
                     /locus_tag="ACG8K3_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9145709.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            6017..6652
                     /locus_tag="ACG8K3_001849"
     CDS             6017..6652
                     /locus_tag="ACG8K3_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9145710.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6843..7586
                     /gene="truA"
                     /locus_tag="ACG8K3_001850"
     CDS             6843..7586
                     /gene="truA"
                     /locus_tag="ACG8K3_001850"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9145711.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7820..8653
                     /gene="msrA"
                     /locus_tag="ACG8K3_001851"
     CDS             7820..8653
                     /gene="msrA"
                     /locus_tag="ACG8K3_001851"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9145712.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8734..9727
                     /locus_tag="ACG8K3_001852"
                     /pseudo
     CDS             8734..9727
                     /locus_tag="ACG8K3_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            10016..10714
                     /locus_tag="ACG8K3_001853"
     CDS             10016..10714
                     /locus_tag="ACG8K3_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9145713.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10864..11319
                     /locus_tag="ACG8K3_001854"
                     /pseudo
     CDS             10864..11319
                     /locus_tag="ACG8K3_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            11400..12526
                     /locus_tag="ACG8K3_001855"
     CDS             join(11400..11628,11628..12526)
                     /locus_tag="ACG8K3_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9145714.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIILKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            12571..13527
                     /locus_tag="ACG8K3_001856"
                     /pseudo
     CDS             12571..13527
                     /locus_tag="ACG8K3_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         4603 a         2210 c         2822 g         4074 t
ORIGIN      
        1 aaatactcgt ttacataaaa aaagattatt tagatgcgat aatatcatag ataaagaaac
       61 attcatcata agaaacagtg atcccaaatt ctgtttcaat atttttgaag gcctttttta
      121 catgaaaaat tgtttcatga gaaggtcttg tgtcatcttc tgatataatt ttaagttcat
      181 gaccgtttat caatgtacgc tcaattagac acgctgtatg aatcaaaagt tttaaaagaa
      241 tggaatgagt gaattttttg tggaaagtac attccaattc ttggataaaa tccattaagc
      301 ttttttggat cttattccca gctaataagt agagatattt gtccactgct tcaactccca
      361 tatcaaaaaa cgatatttct tgtgaatgag tggctgctgc ccttttttga ttgttgttct
      421 tcagtcgtaa gatgacaaag catcttatct ttgaataaaa aaatagaact acctaaggaa
      481 gcgtttcggc gcttcctttt tctatatgat cgtttaatga ctgtaggaat tcagtatacc
      541 aaaagaaaac cgcctttttg aataaacttg atagtggtca attttttatg atggataagt
      601 atctatactt agtctcgtaa gaatgaaaag gaaaagaggg agaaagatga aattacaaaa
      661 gtggatccaa gagcatcgaa atcatatcac cgccttatca gctgtgttga ttatttttgg
      721 ttttatcggg aaatttactg gatttgtctt gttctataac attgctctag ccatagcttc
      781 tattattgcg gtcataccta ttataatcca tgcgtatcag gcagtaaaag tcaaagttat
      841 ctcaattgat ttactcgtta ctattgctgt gcttggtgcc tttatgatcg gagaatacaa
      901 ggagtcagct atcgttacat tcttgttttt gtttggtagt tatctggaac aaaaaacatt
      961 gaccaaaacg cgggaatcaa tcaaggcttt gaccgagatg gccccatcga ctgccagcgt
     1021 tgtccaagaa gatggaacga ccgaagaagt ggaaatcgat gacgtggaag aaggcgatat
     1081 cgtcttagta aaaactggtg gagctattcc tgtggacgga cgagtcattg acgggaaagg
     1141 gtatgcggat gaagccgcag tgacgggtga atcacgacaa gtcgtcaaaa tcagtggaga
     1201 taacgtattt tctggaacga ttttagcaga tggctacgtg acgatcgaag caactaaggt
     1261 cggagatgat acgacctttg ccaaaattat agaattagtc gaagaagcac aagataccaa
     1321 atcacatgcg gaaaaattca ttgatcaatt ttctcagtat tatactccaa ccgttttact
     1381 tattgcgttg cttgttggtc tgatcagtcg tgattttcgt ttagccatca ctgttttggt
     1441 tcttggttgt cctggcgctt tagtcattgg tgcgcctgtt tcgaatgtag caggaatcgg
     1501 taatggagcg aaacggggca tcttagtcaa aggcggtgaa gcaatggata ctttttccaa
     1561 aatcgatact tttgtctttg ataaaaccgg tacattgacc aaaggaacta ctgctgtatc
     1621 cactatcaag aactatacag aggatgaagg ggacagctta gctttagtgg ctcgattaga
     1681 aacgttatcc gatcatcctc tggggcgcgc cgtgatccat tatgctcagg agaaagacat
     1741 tgattttcaa caattagcag tcaaagataa tcagacgatc aagggacaag gaatcacagc
     1801 tgagattgat ggtcatacag tttgtgcggg caacaccaaa ttgattgccg ctcacaaact
     1861 ttctctcaca ccaaagcaag tacaagattt acatcaatta caacaaagag gcagctcagt
     1921 cattattgta gcaatcgaca aaaaaatcac tcaaatcatt ggtgtgtcag atgtgattcg
     1981 accagaagtc gctgaacaat tagctatgtt gaaacaagca ggagcaaaac atttagtgat
     2041 gctgacaggt gataatcaga tgactgctga ttatgtagct gacatgttag gcattgatga
     2101 agtccatgct gaattattgc cagatgaaaa ggttcaattt gtgaaaaaat accaagaaca
     2161 aggattgcac gttgctttca ttggagatgg aatcaatgat tctccctctt tggctgcggc
     2221 tgatatcggg attgctatgg gatcaggaac ggatgtcgct atcgaaacga gtgacgttgt
     2281 cttgatgcaa tctagttttg gatcgttggt tcatgcttac cgtttggcta agaaaactgt
     2341 tttgaatacc cgtgaaaatg tagtgattgc cattggtgtc gtcttatttt tactcatcgg
     2401 cttatttgcc gggtttattt acatggctag tgggatgttc gttcacgaag ctagtatctt
     2461 gatcgttatc tttaacgcga tgcgtttatt gtattttgga cacgaaagca aagaagaaaa
     2521 atcgtaaaac ttgataagaa tcaatttttt tatggaagct gtaatctaga ataagactat
     2581 caagaaaaag gaaggaagaa ctacgatgaa aaaaatgatt atgcaattag atacactagc
     2641 ttgtccaagc tgtatgcaaa aaatagaaca tgcggtttct caacagcctg gggtaagcaa
     2701 tacgaaagtt ttgtttaatg cgagcaaaat caaagcaaac tttgatccag atgaagtcca
     2761 ccctgaacaa ttagctgcag ttgtggaaaa attaggttat gaagtccaat caacaaaagt
     2821 aaaagaggcg taaatcagtg aatatagtgg aagaaaaatt tgctcaagaa acaaaacaag
     2881 cagatatcga tcatcataaa ccaactgctg gcgccatggt ggggcatgta ttggcaaatt
     2941 ttaaattaga agaaatgaaa ttgatccaag cacaattgta tgttttgggt acatcttcgc
     3001 aagaattaag tacccttttt tcagaatatg ctgatcaaga agcgagatgg ttcaataaaa
     3061 tctctcatga gttagtgatt gaaaatgaaa tcgttccgtc tactttagaa gaagtcatgc
     3121 gttatggaaa aatttacgaa catggtcaaa acaaatattt accagcctca acaatgttgg
     3181 aaaactttgc aaaagatttt gatttccaaa atgtctttat cacacgggca atcaaattag
     3241 ctgaaaaaga agaaaaatat ggcttgaaac aattgcttgt tgaactttta agtttcaata
     3301 aagaaatcat ctaccaagta caaagactcc aaggaaaaac tgtccgacaa gatttggatg
     3361 aggaagacga tgatgactaa aagtctatgt ttcttcaaat acaaatataa agcgtgaggt
     3421 aaattggaaa atcgcgaaga ttaaaataaa agaagcaccc gctgatgtat tacagacaac
     3481 atcagcgggt gcttcttagt ttttaaaagg tttaaaagat ggatgagaaa atacaaaaaa
     3541 tcaataaaat cacattgctt atttgataga ataaagaagt gggagtaatc gaaaggagtt
     3601 ttttaattta aaaaaaggat tttagtaaca attggtctag cttttttgtt agctggttgt
     3661 accaacggga atgaatcgaa aaaagaatca acaaaagaaa cagcgaaagc aacaattgca
     3721 actactacaa caactagcac agagagcaag ccaactgcag caagtacgga aacagcagat
     3781 acctctactt cgtcagagtc ttccaaaacg actgaaacaa ctcaaacatc tacttcaagt
     3841 tcttctgtag aggcttcatc ttcagaaagc agcgaacaag tcgaacaaac actgtggaat
     3901 gcagacaaat ccgcacaatt agaggcattt atggctacat ggggccaaac aatgggacaa
     3961 caatacaaaa gctatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
     4021 gttattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
     4081 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
     4141 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
     4201 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt taacgagaca
     4261 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atgggtaagt aaaccagtga
     4321 gagtgagaga aagaagagac tcagtggaaa atattaattg attgctatgt ctaagaatta
     4381 agatgaagga tgaaatttct agtttcgtcc ttcatcttag ttacgtttta cttggataac
     4441 tataataata acgataaata gttttctctc gacttcttta gagattgaat aaaccctcca
     4501 atactaaaat tgaatgttgc aagagcatga attgccaatt gtattattag aatttttaga
     4561 aaattgcacc tttagtttct ttagataaaa tgatacaatg attgttaaaa ttgtgaaagg
     4621 gtggtcattt gttagagaag tttttggata tgtcgttagt tcaacgtatt gttgttggta
     4681 ttttagtcgg aatattgttt ggtatctttt tccccacgtg gacatttatt tcaatattgg
     4741 ggacgttgtt tgtcagttgc ttgaaggcaa tcgcacctat tctggttttt ttcttgacga
     4801 tggcgtcaat tgccaagcat aaaattggga ataagacatt cgtgaagcct attttgatct
     4861 tatatttagt gggaacattg ctttctgcac ttgtagcagt cattgtaagt tcaattttta
     4921 ctgttcctat tactttacaa gaaacagtat ctgaaaaagc accgcaaagt ttggaaagtg
     4981 ttttatctac tatgttgacg aatgtcacac aaaatcctgt tcaatctctt attgatgcga
     5041 attaccttgg cgttttattc tgggctgtat tgttgggact agcttttcga gcacgttcag
     5101 aaccgacaaa agagttggtg gatcagatat ctatagcgtt gtcacaagtc gtacaagtga
     5161 ttattgcatt tgctccgata gggattttag gattggttta tcaatcaatt gctacgactg
     5221 gaatcgctgg attggcagag tatctgcagc ttttacttgt tttgatcggt acgatgctgt
     5281 ttgtcgcatt agttgtgtac ccattcctca cgtttttgtt tattaaagaa aatccctatc
     5341 cattgatttt cttttgtttg aaagaaagcg cacttcccgc tttctttacc cgaagttctg
     5401 ccgcaaatat cccgataaac atgatgttgg ctgaaagatt gaaattgaca aaagagtctt
     5461 attccatatc gatcccgtta ggtgctacga tcaatatggg cggtgccgcg attaccattt
     5521 ccttgatgac gttgactgct gtacattctt tagggattga agcaccattt gttttgaaag
     5581 tccttttatg tattttgtct gctttagctg cttgtggagc ttcaggtgtt gcgggcggtt
     5641 cactgttatt gattccatta gcatgtagtt tgtttggtat ctctaatgat gtcgcgatgc
     5701 aaatcgttgg tattggattt attatcggtg ttatccagga ttcaatggaa actgccttaa
     5761 attcgtcaag tgatctgttg tttactgcaa taggagagct aggcgcgcgt aaaagaagtg
     5821 gggaaaaaat caaattgaaa gattgtttag atggattaag tgaacgttga acgttagata
     5881 tttagtattt ttaaaatgat tcgagttttt aatgagttat atagatgttc gtgtaaatag
     5941 aggtttctgt taaatcaaga taaagtcgtt tataataaca attaaaagca taggtaattg
     6001 ttattggagg cgtatcatgg attttttacg taaatttggt tttttctttt tattattagg
     6061 cgtgctgagt gattttttga ctccgtacgt attaggattg tattatcctg aattagatca
     6121 aatgaagagc gtgatcagtg tatttggaga ggtttccagt cctgtaagaa aagcgtttct
     6181 tatttggtct gtggtttcag ggatcttttt catcctgtca ttgcctgcat tgtatgctgc
     6241 atttgtaagt acatctcgtg tgttagctac cctagttaca gcagccatcg cgttctatgg
     6301 tataggtgat tgtatcttta caggtttgtt cagtatcaat acccatgaat cttcgtggaa
     6361 tgtctcgaca tgggtccata atatcggatc aggtttaggt tacagtggtt ttttattgtt
     6421 tccagttctt cttttcttga tctatcgaaa aaatgggcaa atggacgtaa gccacttata
     6481 ccttggactg tctgtgatca gtttgctttt tgcggcgatt tatggactgg caagaattcc
     6541 ttctataaat tcttggccat tactgaataa aataggtttt tgccaaagaa tcagtttttt
     6601 ctttaattat gtgcccattg cctggttgag tatcttgcat ctcaaacagt aaaacttgag
     6661 ccgtcaaatt gaacagtcag cggatacttc atccggtggc tgttttttgt ttttctgaaa
     6721 tatatatttc cacagttttc ttgtatagct tctgtcttag agaagcaagg agctatattt
     6781 tgtgctacaa tcttaaaaag gtagagagaa gaccaaatgg aagacagagg agccactaaa
     6841 atatgagaaa catcaagttg acaatcgaat atgacggaaa aagatattca ggatggcaaa
     6901 gacttggaga tgacgataaa accattcaag gaaaaatcga aaaagtattg catcagatga
     6961 caaatgaaga gatagaaatt attggatcag gaagaacaga tgctggcacc catgcgcgag
     7021 gacaagtagc aaattttaaa acaaacacag aaattggact ggcagaaatg atcgacttta
     7081 tgaatcgtta ccttccgcgg gatattgtca tcaaaagaat cgaagaaatg cctgagcgct
     7141 tccattcaag atataatgcg gtgggcaaaa aatacagcta ttatgtttgg aacaacgtga
     7201 ttccttcagc atttgagcga aaccacagct tttatttccc tcaagagttg gatatggaca
     7261 agctgaatgc agcttgcgaa aaattagtgg gtacacatga tttcattggt ttttctgcat
     7321 tgaaaaaatc aaaaaaatcc acagtgcgta cgattgatga attatctatc gaacgaaagg
     7381 gagaaatgct tcattttact tttgtcggag acggattctt atataaaatg gtgcggatta
     7441 tcatggggac attactagaa atcggcacag gtgccatgcc gatagaaaca atcgatgaaa
     7501 tatttgaaag caaagtgcgg caacatgcag gagaaaccgt ccctgcacaa ggtctgtttt
     7561 tggatgaagt ttattatcgc tcataaaaaa gaatcatctt tactcgactt ctgagaattg
     7621 gagaacatcc cttttttcag aggtcgtttt ttagttaaac caaaccatag agtatgcttt
     7681 ttaagataaa gatcatctta tatcatctga agctgcttat cttctttgaa taaatacgat
     7741 tcaatcgatt atttcctcac tttatgataa attacagcta aagtcactag cttgtcttct
     7801 tcggaaagga ggagaatcta tggatgagga acaaatattc attcaaacac tttataatct
     7861 gattttgaat ccgaatacac gagattggga acgaaaagta ttgattcaga caaagaatga
     7921 cacaagagaa aatatatccg ttaaagaaca gttatcaaaa ctagaagtaa cactacgtcc
     7981 attagctatc cgaatgaatc tgacgcctga tgtaatggac ttttatttat tgattacgga
     8041 aggatttgat aaggagcaaa aatatgactt ttccaagcat gccatgcaag atgctgacta
     8101 tcaagaacgt gcagtatttg cagggggatg tttctggtgt atggtcgagc cgtttgaatc
     8161 gaaaaaaggg atcctatctg ttttgtcagg atacacgggc ggacatgttg aaaaaccgaa
     8221 ttatgatcaa gtaagtggtg gttacacagg tcatgtggaa gctgtggaga tcatttatga
     8281 cacaagagaa ataagttata gcgagttgct gacaatctat tggcaaatta ctgatcccac
     8341 agatacattt ggacaatttc aagatagagg gaagcaatat cgtccagtga ttttttatca
     8401 agacgaacga caaaaagaat tagcggaaca atcaaaacag aaactagaca gttcaggaac
     8461 tttccaccaa ccaatcgtca caaaaatcga accagccggt acgttttggc cagcagaaaa
     8521 ctatcatcag caattttaca aaaaacaacc gaaacgctat aaaaaaatcc agcaagcacg
     8581 taatcaattt ctgatctatc aacgagtaaa aaacaaatgg caaaaaaata tacgaaaaaa
     8641 tcattttgat taactccttt tctttaaaat gtagtaaact aaaaatagaa aacaagaaag
     8701 cgttttaaaa agatttgatg aggaggagaa aaattgtata ttgcagatag tgcacgttat
     8761 gaaaaaatgg aatatcgtaa atccggtcat tcaggtttaa aattaccgat tttatctctc
     8821 ctctcggatt atggcaaaat tttggtgatt atgatcctat ccataatcaa agagagattt
     8881 taagaggcgc attcgacatg ggaattaccc atttcgacct tgcgaataat tatggaggcc
     8941 ctgctggagc tgcagagaaa aactttgggc gtatttttag agaagatttt caagcctacc
     9001 gtgatgaact gattatttct agcaaagcag gttatcatat gtgggaaggg ccatatgggg
     9061 aatggggctc tcgtaaatcc atcatatcaa gttgtgacca aagtttccaa agaatggggc
     9121 tagattatgt tgacatcttc tatcatcatc gcccagaccc tgatacacct ttggaagaaa
     9181 cagcagaagc gttgatgcaa cttgtaagac aaggaaaagc attatatatt ggtatatcca
     9241 actataatgg agaagataca aaaaaatgac tgaaatttta aaaagaaagg aagcaccgtt
     9301 cattattcat cagatgcgct acaatatgtt ttctcgtgca ctactagaag acgatctatc
     9361 tccagtatta gaagaagaag ggctaggggc aatcacgttc agtccgcttg ctcaagggtt
     9421 gctgacaaat cgttatctac atggtattcc tgaagattct agagcacatc gtaaggaaat
     9481 accattcctt tcagaagaac aagttggatc aactctggaa aaaatcaaag cactgcaaac
     9541 aatcgccgta tcacgaggac aatcattagc gcaaatagcc cttgcatgga atttacgaca
     9601 aaaatctgtt acaagtgtac tgatcggagc aagtcggtta agtcagttac aagaaagtgt
     9661 tcgaatgatg gataatttag atttctcacc agaagaagaa ttgagaatcg accaaattct
     9721 agaatagaaa cgatgtaatc aaacttttta tccatatatt aaaaaaaaca aaagatgaca
     9781 tcaagaatcg aatgaagatc gtctgttatt gaacatcata ttttttgtcg atcaaaataa
     9841 aaatgatcga aggctaggag tgaattttat cctagtcttt tttatggtaa atatcttctt
     9901 cgtttgtatg tctatgaacg gattaatcga ttttttatac aaaaaatcat cagatgattc
     9961 aaacgtgtta taataattac gaaaaaatac atcagatgaa aaggaggtat gtggtttgga
    10021 agaaaataaa accctagtag aagtaactac tgacaagatc cttagatatt taaaggaaat
    10081 gcagttgcaa gtaggagata aactgccgaa tgaatatgaa ttgtccgatt atttagaggt
    10141 ggggcgaagc acggtgcgag aagctgtgcg ggctctagct tccaggaata tccttgaagt
    10201 tcggcaagga gcggggacat ttgtcagtga taagcgaggg attgccaatg acccactcgg
    10261 tttttctttg attgaagatc aggatcggat gatacgagac ttatttgaac tgagatattt
    10321 attggagcct aagatggcag gtttggcagc ggaatatgca acgaagaatc aaatcgtcca
    10381 aatgaaaaaa ttaacgcaaa agattgaaac ttcttttcga gaaggcacac aagaacatgt
    10441 tcaattagac atcgattttc atacgatgat tgcagaagct agcggaaaca tagccttgct
    10501 gcatattatc ccaatcatta atgaatcgat tgctttgttt aataaaaatt atgatatcca
    10561 gcatctgaaa atcgagacga tcgatattca tacagagatc gttcaggcca tcgaaaggcg
    10621 gaacgtactt gctgcgtttg acgcaatgac tgtccatatg gcaataaatc ggagagaatt
    10681 acagaaaata ataggagaaa aagaaactaa ttgacagttc atctgatgta ttttatgata
    10741 aatacatcag atgatttttt gttttatatt tggtaaaaaa gaggagtaaa tagtttctgt
    10801 tcaccaaaat gtaagcgctg actaatagac aaaaagagat gacgtcaagg agaagaagca
    10861 ggaatgaggg gatctggaat gagttttatg gataaattag ccaaagtttt agggaacgtg
    10921 gcaacgagaa tcaataattt aaggtatatc atggtgataa aaaacgcatt tgctgcattg
    10981 attccggtaa ttattacggg tgcttttggc acattgtttt cagcaatggt ctttgactcg
    11041 gaaaatggct tagcacagat aagttacttg cgttttttag aagaattaaa gcctatcgca
    11101 tctgccatca gctatgtcac actaagtttc ttgaccatct atgctgtttt tctaataggg
    11161 atcgaattag ccaagctgaa taaagtggat ggtgtattcc ctggtatcat cgcagtgatg
    11221 tcttatttat ctgttaatcc aactgtttat gaattagtga atgaaaatca gactgttata
    11281 gtagaaaacg tattggctaa acaatataca gacacgaaag tggtgttagt atcaaatctt
    11341 agacagcttt tcgtagagac aagaataaat taaacttatc tacgagagga tgaattttta
    11401 tgacccgata tgaagaaaat tttaaacaaa tgattattga actgaatcaa actggacgtt
    11461 ctgttcgagg gttagcgaaa gaatatggct tatctgaagc aacgatttac aaatggaaga
    11521 atttatattt acctgatcag tccacaggac tgactggaaa agaagtagct gaactgagaa
    11581 aagaaaatgc tcgtttaaat gaggaacttg aaatcttaaa aaaagccgca gccatattct
    11641 ctcggaaaac ctaaattcgc ttgttcaatt tattgaaaaa tggtgtaagg attacactgt
    11701 ttctttgttg tgtcggttat tagaaatccc tagaagtgtc tattattttt ataagaataa
    11761 accgttgaca gctacagaaa tcagaaataa taagttgaaa aagaagattt caacaatttt
    11821 ttttacgaat aaacaacgct atggtgccac aaagatccat caagttttat taaaagaagg
    11881 gatttcagta tctcttaaac atgtccaaaa gctaatgaaa caattaaatt taaggtcgat
    11941 tgtagttaaa aaatatagac ctcaaaggtc taataaaccg atcattttaa aagagaatct
    12001 cttaaatcag gacttttcta ctgaaacgat ctgtgagaaa tgggcagctg acattacgta
    12061 cattcctact aagaaaaatg gttggtgtta cttatcttca atcatggatt tacacacgaa
    12121 aaaaattatt agttatacat tttcaaaacg aatgactgtg gattgtgtca ttcaaacatt
    12181 gaataaagca aaaatacatt atcacattcc agaaggaatg attctacaca ctgacttggg
    12241 cagccaatac acagcaagag aagtggaaca atggcttaaa accaacaaaa taaggcattc
    12301 ttatagtaga aaaggaacac cttatgataa tgctggaatc gaatctttcc atgcctcatt
    12361 gaaaaaagaa gaagtctaca cgactagcta ctcagatttt gaagaagcaa atcgagcgct
    12421 atttagctac attgaaggat tttataaccg aaatcgaatc catagctcga ttcactatct
    12481 aaccccacag gaatttgaag aattagcaaa agaaaaaatg gcttaaccgt ctctactaaa
    12541 atgtgtccaa gatattgact caaatccatt gatcgttacg gtgatagggt aaaaaactgg
    12601 tttacattca acgaaccaat cgttccccaa acgcgttgct atttggatgc tgttcgctgg
    12661 ccgcatgaac aaaacacaaa aaagtggatg atgtggaatt atcataaagt tttagcaacg
    12721 gctaaagcag tagagattta ccgcagcgga aattatggtg gaaggattgg ctgtatttta
    12781 aatcctgaaa tggtctatgc tcgatctaat tctccagaag accaaaaagc tgcttatatg
    12841 tatgatttgt tttataatcg agtatttttt gatccgatgg taaaagggta ctacacagaa
    12901 gagttcctgg agttgtgtca aaaacaatcg atttatttcg atcctgcaac agaggattta
    12961 gaaacgattc agcatcatac agtagatttc cttggaatca atcaatatta tcctaaacgc
    13021 gttcgcgctc ctcgttatca atggaatgaa gagacacctt tccatccaga acgatattat
    13081 gaagtctttg atttacctgg gaagaaaatg aataattcca ggggatggga aatctatccg
    13141 aaaatcatgt atgatatagc catgtatcta aaagaaaatt atcataatat cccttggctg
    13201 ataacagaaa acggtatggg aagagaacat gaagaacggt atatggatga gaaaggaatc
    13261 gtgcaagatg attaccgtat cgaatttatc ggacagcatt tgcgttattt gatcaaagca
    13321 acagaagaag gcgcaaattg cgaaggctat atgttatggg cattcactga ctgtgtgtca
    13381 ccaatgaatg catttaagaa tcgatatgga ttagttcgaa tcgaattgaa cgatgaacga
    13441 agcagaacat tgaaaaaatc tgcttcgtgg tatcagactt tgattgataa gagagaattg
    13501 gatttgcctg acgaaatcta taaataaata gaagtttttt gtagatgttc attgtattta
    13561 ctggttattt gaaataaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaata
    13621 gcaaagtaaa aacaacatct tatgttttta acttggttgg acttattacc gcagatgcaa
    13681 gcttttgaat accgtttatt cgaaaaaaa
//
LOCUS       DAZKCN010000065        13706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000065 DAZKCN010000000
VERSION     DAZKCN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.066"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..902)
                     /locus_tag="ACG8K3_001857"
     CDS             complement(<1..902)
                     /locus_tag="ACG8K3_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP9145715.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ACG8K3_001858"
     CDS             1185..2153
                     /locus_tag="ACG8K3_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9145716.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ACG8K3_001859"
     CDS             2266..2544
                     /locus_tag="ACG8K3_001859"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9145717.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ACG8K3_001860"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ACG8K3_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9145718.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ACG8K3_001861"
     CDS             3001..3756
                     /locus_tag="ACG8K3_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9145719.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ACG8K3_001862"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ACG8K3_001862"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9145720.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ACG8K3_001863"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ACG8K3_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9145721.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ACG8K3_001864"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ACG8K3_001864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9145722.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ACG8K3_001865"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ACG8K3_001865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP9145723.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ACG8K3_001866"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ACG8K3_001866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9145724.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ACG8K3_001867"
     CDS             8493..9035
                     /locus_tag="ACG8K3_001867"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9145725.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /gene="atpA"
                     /locus_tag="ACG8K3_001868"
     CDS             9058..10614
                     /gene="atpA"
                     /locus_tag="ACG8K3_001868"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9145726.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ACG8K3_001869"
     CDS             10628..11530
                     /locus_tag="ACG8K3_001869"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9145727.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ACG8K3_001870"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ACG8K3_001870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9145728.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ACG8K3_001871"
     CDS             12973..13395
                     /locus_tag="ACG8K3_001871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9145729.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2452 c         2988 g         3736 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag actttt
//
LOCUS       DAZKCN010000066        13376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000066 DAZKCN010000000
VERSION     DAZKCN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.067"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACG8K3_001872"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACG8K3_001872"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9145730.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACG8K3_001873"
     CDS             1013..2260
                     /locus_tag="ACG8K3_001873"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9145731.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACG8K3_001874"
     CDS             2336..2680
                     /locus_tag="ACG8K3_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145732.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACG8K3_001875"
     CDS             complement(2797..3123)
                     /locus_tag="ACG8K3_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145733.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4697)
                     /locus_tag="ACG8K3_001876"
                     /pseudo
     CDS             complement(3496..4697)
                     /locus_tag="ACG8K3_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4814..5329
                     /locus_tag="ACG8K3_001877"
     CDS             4814..5329
                     /locus_tag="ACG8K3_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9145734.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5528..5974)
                     /locus_tag="ACG8K3_001878"
     CDS             complement(5528..5974)
                     /locus_tag="ACG8K3_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9145735.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="ACG8K3_001879"
     CDS             complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="ACG8K3_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9145736.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7853..8482)
                     /locus_tag="ACG8K3_001880"
     CDS             complement(7853..8482)
                     /locus_tag="ACG8K3_001880"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9145737.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8466..8930)
                     /locus_tag="ACG8K3_001881"
     CDS             complement(8466..8930)
                     /locus_tag="ACG8K3_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9145738.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9417..10685)
                     /locus_tag="ACG8K3_001882"
     CDS             complement(9417..10685)
                     /locus_tag="ACG8K3_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9145739.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10916..11653)
                     /locus_tag="ACG8K3_001883"
     CDS             complement(10916..11653)
                     /locus_tag="ACG8K3_001883"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9145740.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11720..12289)
                     /locus_tag="ACG8K3_001884"
     CDS             complement(11720..12289)
                     /locus_tag="ACG8K3_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9145741.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12417..13289
                     /locus_tag="ACG8K3_001885"
     CDS             12417..13289
                     /locus_tag="ACG8K3_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9145742.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         4036 a         2753 c         2427 g         4160 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc
     4441 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
     4501 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
     4561 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
     4621 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
     4681 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
     4741 aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
     4801 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
     4861 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
     4921 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
     4981 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
     5041 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
     5101 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
     5161 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
     5221 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
     5281 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
     5341 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
     5401 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
     5461 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
     5521 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
     5581 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
     5641 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
     5701 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
     5761 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
     5821 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
     5881 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
     5941 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
     6001 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
     6061 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
     6121 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
     6181 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
     6241 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
     6301 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
     6361 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
     6421 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
     6481 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
     6541 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
     6601 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
     6661 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
     6721 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
     6781 ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
     6841 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
     6901 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
     6961 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
     7021 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
     7081 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
     7141 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
     7201 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
     7261 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
     7321 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
     7381 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
     7441 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
     7501 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
     7561 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
     7621 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
     7681 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
     7741 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
     7801 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
     7861 acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
     7921 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
     7981 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
     8041 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
     8101 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
     8161 gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca
     8221 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
     8281 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
     8341 gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
     8401 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
     8461 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
     8521 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
     8581 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc
     8641 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
     8701 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
     8761 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
     8821 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
     8881 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
     8941 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
     9001 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
     9061 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
     9121 attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca
     9181 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
     9241 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
     9301 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
     9361 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
     9421 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
     9481 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
     9541 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
     9601 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
     9661 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
     9721 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
     9781 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
     9841 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
     9901 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
     9961 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    10021 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    10081 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    10141 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    10201 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    10261 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    10321 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    10381 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    10441 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    10501 attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct
    10561 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    10621 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    10681 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    10741 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt
    10801 tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    10861 gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt atggattata
    10921 cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta
    10981 tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt
    11041 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    11101 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    11161 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    11221 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    11281 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    11341 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    11401 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    11461 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    11521 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    11581 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    11641 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    11701 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    11761 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    11821 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    11881 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    11941 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    12001 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    12061 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    12121 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    12181 ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg
    12241 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    12301 cttttatcct tactttataa cggacggctt atcatgtcta atacttataa aacatactta
    12361 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    12421 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    12481 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    12541 aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg gattctggtc
    12601 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    12661 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    12721 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    12781 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    12841 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    12901 aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc
    12961 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    13021 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    13081 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    13141 ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg ccattacaag
    13201 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    13261 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    13321 taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt tcttaa
//
LOCUS       DAZKCN010000067        13310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000067 DAZKCN010000000
VERSION     DAZKCN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.068"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1328)
                     /locus_tag="ACG8K3_001886"
     CDS             complement(66..1328)
                     /locus_tag="ACG8K3_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145743.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1321..2220)
                     /locus_tag="ACG8K3_001887"
     CDS             complement(1321..2220)
                     /locus_tag="ACG8K3_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145744.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2365..4143)
                     /gene="uvrC"
                     /locus_tag="ACG8K3_001888"
     CDS             complement(2365..4143)
                     /gene="uvrC"
                     /locus_tag="ACG8K3_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9145745.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4325..4639)
                     /gene="trxA"
                     /locus_tag="ACG8K3_001889"
     CDS             complement(4325..4639)
                     /gene="trxA"
                     /locus_tag="ACG8K3_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP9145746.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4726..7086)
                     /locus_tag="ACG8K3_001890"
     CDS             complement(4726..7086)
                     /locus_tag="ACG8K3_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP9145747.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7201..7749)
                     /locus_tag="ACG8K3_001891"
     CDS             complement(7201..7749)
                     /locus_tag="ACG8K3_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFP9145748.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7807..8244)
                     /gene="zapA"
                     /locus_tag="ACG8K3_001892"
     CDS             complement(7807..8244)
                     /gene="zapA"
                     /locus_tag="ACG8K3_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9145749.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8446..9363
                     /gene="rnhC"
                     /locus_tag="ACG8K3_001893"
     CDS             8446..9363
                     /gene="rnhC"
                     /locus_tag="ACG8K3_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP9145750.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9619..10530)
                     /locus_tag="ACG8K3_001894"
     CDS             complement(9619..10530)
                     /locus_tag="ACG8K3_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9145751.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10933..11511)
                     /locus_tag="ACG8K3_001895"
     CDS             complement(10933..11511)
                     /locus_tag="ACG8K3_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9145752.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11531..12199)
                     /locus_tag="ACG8K3_001896"
     CDS             complement(11531..12199)
                     /locus_tag="ACG8K3_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145753.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12214..13284)
                     /locus_tag="ACG8K3_001897"
     CDS             complement(12214..13284)
                     /locus_tag="ACG8K3_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145754.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3780 a         2862 c         2197 g         4471 t
ORIGIN      
        1 aggtctatac aaaaaagaag ctgggaacaa taaatgtcca gcttccttct atatttttct
       61 atattttatg ctttttttcg ttcatttctc ataattgaaa tcgattgttt caatgaactc
      121 cataatccgc cttcgctata cacgagtaca ttcgatttgt acatgtttgc agacaagatc
      181 atcaataaga atgttacggc tacaagtacc aacaaggaaa tcaatgctcc acccataccg
      241 actgtctcat ttgctaagcg aaccggcata atatatgaag ataagaatgg aatatacgaa
      301 gtgatccgaa taacgatatt gtttggatct gaagctccta gtatcaatcc aagcatatat
      361 ccgcctagag atagataagt cactggcaaa attgcttttg ctgtatcttc tgctttgttg
      421 accaaagagc cacataaagc tgctaaaaca gaaaaaatca aaatgccaaa gatcatgaat
      481 agaagagaat accacaagaa tggtccaatc aagttatcca atgagatacc gtctaatata
      541 tttttgacca tttccatatc tttgaattga ctgtatccta taccaaaaat aataccgtat
      601 aatgccattt gcgttaatgc aacaagtaaa acacctgtta gttttccgta aaaatgcttt
      661 tgtgcagtcg tactggacag gatcacttcc atgatccgcg tgcctttttc agaagcgatc
      721 tcttgtgcaa tgatctgcgc atacgtcaaa ataataatga acaagacaat cgttgctaca
      781 taacttacga catattggac tgtcgagtta tcttctccaa ctgtcatttt tccttttgaa
      841 tcaaaattga ctttttgtcg agtcaaactt gcttgttggc tcaagctagc tacttcttct
      901 ggtgaaatgc ctaattgact tgctcgaagc atcgattgca agccagtcag ttgttgttgt
      961 atcagtagtt gtgtactttg tcctaatgaa ttttcgctat aaagcatccc atttatctta
     1021 tcattctcag ttgagactac taaatacgca tcgatttttt cttccgaaag ctctttttgt
     1081 gctttttctt cagaatcaac tgctttgaac tcaaaatcat ctgttttgac ttggctcatt
     1141 tgctgactga tttgctcatt atcagccaat acgccgattt tattgacatc actattttgt
     1201 tgagcaaagt ttcctgctac ataaatgata cccagcatga taaaagggac taatatcatg
     1261 atcaagaaag aaatcgactt aacatttttt aaatagacat ctttggtaat aatccaaaac
     1321 ttacgcatcg atttcacctg ctttcaattt gaaaatctct tccaaagttg gcggttgctg
     1381 gttaaacata ggaatgtagc cgaactgagt cgcacgagta aagatttctt tcccagcttc
     1441 aggatcttct aaggtaatct ctaacgtatt gtcttcatga aggatcactt ttttcacgcc
     1501 ttcgatttct tctacttcct gttggcttaa accagattcc aagaataact tcgtacggcc
     1561 aaatgattca cgaatctcat gtacttttcc attcaaaacc atttttccgt ttcgcaacat
     1621 gatcaaatga tcgcagattt tttctacatt atccatattg tggctagaaa aaatgacaca
     1681 tgaaccatgt tttttcaatt ccaatattcc atcttttagc aattcagcat ttaccggatc
     1741 taacccgctg aatggctcat ccaaaatgat caatttaggt tcatggatta acgtggcgat
     1801 cagctggact ttttgctgat ttcctttaga caaagatttt actttatccg tctttttccc
     1861 ttttacctga aatttttcca tccaaaaatc gatttttggt tcaatctctt ttcttgattt
     1921 ccctctaagt tctgcaaaat aaagcagttg gtcttgaatc gttactttcg gataaagtcc
     1981 ccgttcctcc ggaagataac cgatgacatc ataatctttc tccccaagat gatggccatt
     2041 ccataacact tctccttggt cttgtgtcaa aaaatccaag atcaaacgaa aagtcgttgt
     2101 cttcccagcc ccgttttggc caattagccc catgattttt ccatcgggga tttggaaaga
     2161 gacatgatcg acagctgtgt agttcccaaa agttttaaca agatccttga cttctaacat
     2221 ttcttcactc cttcttcaag aaatcacata tatccttaga caccagacgt aagcgattta
     2281 ttatttacca acaaaacgta aacgctcttt ttgttgtaca taaaatgata ggacacttta
     2341 ctttctttgt aaagtgccct ttcctcattc ctgatgtaat ttatcaaata cgttttgtgc
     2401 gacatttttc ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg tgatattttt
     2461 caacgatttg aactctttaa gcagcatttt ttttcgtttt ggtcctaacc cttcaatttc
     2521 atctaatcgg gaagcaaaac tgtttttact gcgtagctgc cgatggaaag tgattgcgaa
     2581 tcgatggact tcatcttgga ttcgctggag taagaaaaac tctggtgaat ttcgctcaag
     2641 cggtactaca gccaaatcta taccgaatag caactcattt gttttatgtt tatcgttttt
     2701 tgccatgcca gcaataggaa tatcgattcc taattgattt tctaagacat cttttgccac
     2761 atctacttgt ccttttcctc catcgatcaa gatcaaatca ggtaaaggca acccttcgcg
     2821 aatcactcgt gaatatctgc gatagatgac ttctctcatt gatgcataat catccggtcc
     2881 gttgactgtt tttattttat atttccgata ttcttttctt gccggttttc catcaatgaa
     2941 tacgaccatc gcagccacag gattcgtccc catgatatta gagttatcaa aagactcgat
     3001 acgtattggg gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc cgatcgtccg
     3061 ttcctgtttt ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg cattcttatt
     3121 cgccaattca actaattttt tcttttctcc tcgttttggt tgcagcactt tcgtagctaa
     3181 caaagcttct acactgggct tgtcgatcgt atcaggaatc aagacttctt taggaatgaa
     3241 atgctcattt tcctgataaa actgaccgag gtaagtcaag aaatcttctt ctgcttcgtt
     3301 atagaaagga aacattgaaa catctcgttc aattagcttt ccttgtctta cgaaaaacac
     3361 ttggacacac atccatcctt tgtcaatcgc ataaccaaat acatctcgat ccagtaaatc
     3421 agtatttgtc attttttggc gagtcatgat tgtctcaatg gcacggatct ggtctcggta
     3481 ttctgcagct ttttcaaact ccatgttttc agcagctttt tccattttct gatggatctc
     3541 tgactcgatt ttttcgtgtc ctccgtttaa aaactttttc acttcatcaa cgattttcgt
     3601 atagactttc ggatctacgt cgaaataata aggacacaga cattgtccca aatgatagta
     3661 aagacaaggc tccttcgtct gactaggttt gcattttctc agaggaaata atcgatcgag
     3721 gatcttcttt gtctcattcg ccgcaccgac atcgggatat ggtccaaaat acagtgcttt
     3781 gtcttttagt actttacgag taatcaccaa acggggatat ttttcattcg ttatcttaag
     3841 gaaaggatag gttttatcat cctttaacat aatgttgtat ttaggatcat ttttctttat
     3901 cagattgatc tctaagagca atgcttcgat attcgattct gtaacgatgt attcaaagtc
     3961 ttcgatttca ctaacaagcc gttccgtttt cgtattatgg ctgccagtaa aatatgagcg
     4021 gacacgattt tttaatattt tggctttgcc gacataaatg atcgttccat ttttatcttt
     4081 catcaagtaa cagccgggct gatcaggaag taaagctaat ttgtttttga ttcgttcgtt
     4141 catctggtac tccccttcat attcaattaa agttattata gcatgtctgt caaaaaaaca
     4201 atccagcctg acaacgtaat tatactcaaa aaatattaat ttgaataacc ttaactcaac
     4261 cgcaaaaaaa ccgaggacgg aactggattc agtcccaccc tcagcttatt tttgctgtaa
     4321 gtttttataa atattgatcg atcatttgac gtagttgttc ttttgaatga acaccaacag
     4381 ctttttctac tacttgacca tcttttttca acattaatgt tggaatgctc atgataccga
     4441 attgttgagg agtttctgga ttttcatcca catccatttt agcaattctg aattcttctt
     4501 catcatattc ttgttccaac tgatccaaaa tcggtgcttg catacgacat ggaccacacc
     4561 aagttgccca aaaatcaatc aatacaagtc ctttatctgt ttcttcatta aatgtcttat
     4621 ctgtaattac ttgtgacata tcaaagacct ccatttttaa aatctatgat tagtatagca
     4681 tttctatact tggatagcga accatctgct tacctttagt aagctttact taaatttaac
     4741 aattgtcgca ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt tgacgctccg
     4801 atggttttta agataatcag taattccctt gcgaagtgcg cctgttcctt ttccgtggac
     4861 aatagtcact tgaggataac cagctagaat ggcagaatcc aaatattgat ctacctcact
     4921 tagcgcttct tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt gactactaga
     4981 ttcagatcgt acagcatgga taactcgttg tgtcggctct ttttgaggag caacaggcgt
     5041 cagctcgtct tcggacacat tcatttttaa tataccgatt tctacttgcc actgattccc
     5101 gtttttacgt aaaagcgtcc ctctttggcc gtaagtcgta accaacactt cgtctcccgg
     5161 tttcaacgtt ttttgttctt tcgccttctt caatacttta tttttcttca aagtttcttc
     5221 ttgatgtaga tttgctaatt gggtctttgc atcgatcaac tgatgctctt tcacatttcc
     5281 ttgcccaatc tgctgttgca ttttacgaat atcagcaatg attttttctg ctttttcttc
     5341 cgcctctgac acgacttcgt ttgctttttt ctttgctttt tccatttctt tttcacgttc
     5401 ttcaaagaaa tagctgtaag cttctttcaa atcatcgtga agttcttgtg cctcagaaac
     5461 aaaatgacgc atttccagat attcggtctc tgccattttc cggcgattct ctaaatctgt
     5521 gatcatttcg tttaaatctt ggctctcatc attcatcaac tgcttcgctt catcaataac
     5581 ttctgtatca agacctaatc ggctagagat ttcaaacgca ttgcttcgtc caggaacgcc
     5641 aatcagcaaa cgatacgttg gacttagtgt atccacatcg aactccatgc tggcattaat
     5701 cgtatttgcc cgattgtatc cataaacttt caattcagga taatgcgttg ttgccatcac
     5761 ataagcagac tttttaccaa gatcatctaa gattgcgatt gctagagcag caccttcttg
     5821 cggatctgtt cctgcaccga gctcgtcaaa taataccaga cttttttcat tgacttttga
     5881 taaaatatct acagtgttcg tcatatgaga agaaaaggtc gatagacttt gctcaatcga
     5941 ctgttcgtct cctatatccg caaatacttc ctcaaaaata ccgatttgac tttcttcacc
     6001 tgcaggaatc ggtaatcctg cttgccccat cagttgtaac agacctaaag tcttcaacgt
     6061 gatcgttttc ccacctgtgt taggtcctgt aatgacgatg gcttgatagt cttttccgat
     6121 tgtgatgtca ttagaaacta ctttttcctg atcgatcagt ggatgtcttg cttgtttcaa
     6181 gacgacatga ttgtcttcac tgattcccgg cacgatggct tttacttctt taccaaatcg
     6241 tgcttttgca ttcatcagat ccattttacc aatcacataa gcattgtgga tgatttcttg
     6301 acgatgggga acaagttcag cagaaagttc acttaaaata cgttgaatct catttcgttc
     6361 agcgatttgg tattgacgca aacgattatt caaatccacc acttgttttg gttcaataaa
     6421 taaagtctgt cctgaagcac tttgatcgtg gactacaccg ccaaatacac cgcggtattc
     6481 ttgtttgact gggataacgt agcggtcatt tcgcatggtg atgatcgcat cacttaaata
     6541 ctttgcattt ttcccacgga cgatcccatc taactgttca cgaacagctt gttcacttcg
     6601 ccgaatgttc tgtcttatgc tctttaactc aggagaagcg tcatcagtca cgcgtccgtc
     6661 ttcatcgatt gcttccttta aacggcggct caaaacaggg atcgtgacta actgatctgc
     6721 ccacatatac aaacgtgcaa attcgatttc actgtcagaa agatcatcaa tgaaacgatt
     6781 caattcgctt gtcgtagaca atactctgga aacttgtgca agctccactc cattcaagtc
     6841 tgctccgatc tcgatccgtt tcatatgggg acggatattt tcaagtttag gtatagggat
     6901 cccgcctcgc agacgttgca ctttcaatcc atcctcagtt tcttgaagcc agtttgtgat
     6961 cgtttgtttc tcactcactg gtaccaattc tgccagttct tcttttcctt gtgcagtcac
     7021 tacaaattgt ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa ggattcggtt
     7081 attcatttta tcaccttatc tttcactatg aaaaaagagc tgtgccggac atacgctacg
     7141 cagccttctt aaataaacta ctctgtacaa aaaacgaaat attttccagc actattttcc
     7201 ttaattcaaa atattcgtta cccaaagctc atacatcttg ttggtaagaa atggcgttct
     7261 ctcaacgata aaacgagcga aactatgctc gccgaattga ttttggacca catctaatgg
     7321 aacaaaggca agtaactgca aaatcaaaaa tatagtaacg taagccatga ttagtgacag
     7381 gatgcctcct gcaatccagt ctacctgtct caagatcggc acatacgtca atccatggac
     7441 aaaaaccccc acaaatcggg taagcagcca gccaattagc aaaataatca gaaaagcagt
     7501 tcctgcataa aaagcttcat ctaggtgaaa agcaaatacc tgatcaaaaa atgctaactt
     7561 tgatgttggg gtgacagctg ggtatggaat gtataattcc aaatgacttg ctaatggttt
     7621 gtaaaaatgc tgggcagcaa taaatgacaa tacatatcca atcgcgaata tgatttgcaa
     7681 agcaaacccg cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata aaatcagcaa
     7741 actcagcatg ctacgttcct ttctcttcta acttttttaa atttttctta ttttgataaa
     7801 aggatcttaa ttagaagatt tttgtttaat ttgtcctttt cgtttctcgt ttaaaatctg
     7861 ttgtgcttcc acatgatttt taatggtatg atctgcttga ccattttctt tcaatacttc
     7921 ccgagcttcg ttctcgattg cttcaatgcg tttcacacga ttttcaagct ctgtaaattt
     7981 gatcattttt ttctttaatt ctgctgtttc ttcttccagc tccaagattc tttcttgttt
     8041 cttcaattga tccgaaagcg cattgacagc catcaagatt gctgcttgtt cattatccat
     8101 ttgtggtgat aattgtttca attctgctaa ctgttcattg atcagcttcg ttacgatatc
     8161 catatgatgc ttggtttccc gtccgataat agtatatgtt tggttggcaa tcactgcttt
     8221 gtagcgtgtt ttttcgtgcg ccatacgaat catttcctcc tatttgatga aagttttatt
     8281 tagtgtacca tgaagttggt tctttcaccg ttctccacaa atatttttca catacttaac
     8341 tattatatga taaactttaa aaaaatgcta tgtctcgcaa tctcttagaa aacgaaaagt
     8401 cgtgagttgc ggtatagttt tatgtataaa aacgaggaga attttatgag caacgtcatt
     8461 atcaaagcaa caaacgacca acttagtcaa atgaaagcat actatcgctc ttatttgtta
     8521 aataaacaga ttccttatac tagctttgct gcaaaaaaag acgggacgac cattaccgct
     8581 tatacatccg gaaaagtgat gtttcaaggt acgaatgcag caatagaagc tgataaatgg
     8641 ggggaggcac aggataaaca agccaagcca gcaaaaacag cctcttcttt gccaaaaaat
     8701 atcagtcaat tatccgtttt aggtagtgat gaggtcggaa atggcagcta ttttggtccg
     8761 ctaacagtat gtgctgctta tgtgaaacgt gatcagcttg cagaattacg taaacttggt
     8821 gttagagatt caaaagagtt aaaagatagc caaatcatcc agcttgctga catcttaaaa
     8881 aaaaccatcc cctataaact gcttgtctta tctccagaaa aatacaatga gatccaacca
     8941 aaatacaatg ccgttcggat gaaagtcgct ttgcacaatc aagcgatcca tttgctgcta
     9001 aaaaaaatcg agccagaaaa accagaagcg attttgatcg atcagtttac gccggaagca
     9061 aattttaaaa aatatgcgcg tttagaaaaa aatcaattac aagaaaaact gtacttcgtt
     9121 actaaaggag aacaatatca tttagctgtt gctgctgctt ctatcatcag tcgtgccagc
     9181 tttttggaag agttacacaa agcatcagct gaagctggaa tcacccttcc atcaggagct
     9241 ggaacgaaat ctgaccaagt agcggcaaaa ctattggaga aaggcggcat gcctatgctt
     9301 gaaaaatatg ccaaacttca ttttgccaat acagaaaaag ctttgaagct gctaaaaaaa
     9361 taatggaact gtgacaaaag tcatgtcaca gtcccttgtt ccgaataaac ggtgttctca
     9421 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
     9481 ttgctccttg tgacgcggag ttaaacggca ggctcacaac ctcttcctta aacggtgttc
     9541 aaaagcttgc atctgcgata ataagcccgg ccaaacgaaa aatatgagga gctatttttg
     9601 tttggctggg cttatttatt actgaaaata agtggtcaat gcttgtgtca aacttgcagc
     9661 aatctgctgc tgataactgt ctgttactaa ttcctttaaa tcactatcat tattcatata
     9721 acccatctcg attaaaatag aaggttgaag attttctcga agaacttgat agttcccagt
     9781 ggagattcct cttgaatcca gaggcagatc agtgaaggag tcagcaattg tctgtgctaa
     9841 tgtttcctct tgtccatagt aataatatgt cgtgattcca gttgcttcat tcgcatattc
     9901 cgtagagtct gcgtgtaaac tgataaagac atctgccttt ttttcttcac tgtagtcgca
     9961 tatatctcca agctgtacta attcatctgt agtatgggtc aaataaaccg ttgctccagc
    10021 atcctctagc gcctttttta cttttatggc tgtacttaat gttatatctt tttcttctgt
    10081 ttgttcatca ttgcttaatg ccccagtatc atttccgcca tgcccaggat ctaatacaat
    10141 cactgcatca gatagctctt ccggttttgt ttgtatattc tttgtgactt tggttccatc
    10201 ttcgagggct aaatatccag tatagccatc atttgtcttg atctgataag cattttttgt
    10261 ttcagaaaga aactccgcca gctctccgcc gtttatagtt gcaagtgggg tagttagcga
    10321 gtcatctgta tatagtgtac ggtcattttt tccgattttg attaatgtct cttgttctgt
    10381 atcatcaaat gattcctttg tctttgtaac cgtactgttc gtactgctga ccgtttgtcc
    10441 aactatcttt ttttgaccaa aaacatctat ttgaaaacag ataataatga taatgattgc
    10501 aaatacaaaa gctccaatta tttttttcat tcttgactct cacctcgtag atccagacta
    10561 agggtcaaaa cttaaaatca gcttaagtac gcaaaaaagg ggctgtaaaa gatataaccg
    10621 tagaaaataa cattactgct aaaatgattt ttttcatttt tggcatatcc tgaaatctcc
    10681 ttttgactat tcgtggtgat tcatctaccc aataaatagt ttccttcatt ctacaaacaa
    10741 ttaatttctt cgttattaaa ttaaaacact tcctggaaca ccgacataat aaataaaaat
    10801 ataccctgat aatcatgaga gaataacaaa tttgtattta tgaaaaacta cctatataaa
    10861 aaaaacaaga tataacgatc ttcgtcatat cttgcttctt cacctaacta tttttctccc
    10921 tatataacag ctttacgctt ctcctaatac accattcaaa tataaatcga tcaattgcgc
    10981 cacatttagc ggttcttcca caggtctttt cgaccgagtg atgtaaggtg ctaacactaa
    11041 gaaaaaatgc cttgctgcga tttcttttga aatcccattt cttagcacag attggtttgc
    11101 tgaaaagatt tcttcaaaag gttcacgata atttttcttc catagaaaga aaagatgatc
    11161 tcttgtttcc tttgaaagat tgctttcaag atcgtgaagc atcagaaaca aatcgaccag
    11221 atgtgtctca atcaaaaact gcgccatttt tgtcaaggtt acttgaaatc ccgtttcgct
    11281 tgtttgaacg atttctctca aagcttttcc aacatctttt aagtggtttt ctaaaactgc
    11341 cgtatatagt ttttctttat caccgaaata atgatagaga ttgggttggg tgatcccaac
    11401 tttttgtgcg atctctcttg ttgaagtgcc gagatatccc ttggacataa acaagtcagt
    11461 cgcttctttc aaaatttttt gctgtgtatc tttgttgtat cgatggacca tttttccacc
    11521 tgctttttgg ttattctttt tctttcaata caccatcttc catcataaat attctatcgc
    11581 atttgtcaat cagccgtgta tcgtgagtaa ccatgatcgt tgctttattt ttgtcttttg
    11641 attcttttgc caagatatcg actacttctc gtgccttttc tgaatccaaa ctagcagttg
    11701 gttcatctgc tagaataatg gacggatcat tgtacaatgc acgagcgatt gcgacccttt
    11761 gccgttctcc accagatagt tcttctggat acttgttgat caacttatcc acgccaagct
    11821 gctcaaataa ttcttttgct tctttttgac gatgttcatg tttgatctta tctactaata
    11881 ataactgctt ttttactgtc agaaaaggaa ccagattgga agcttgcaaa ataaagccaa
    11941 tttctttaaa acgtagttta acgcgttcac tttctttttg attactgaat tcttgatcat
    12001 tgataaatac agagccactg ctaggcgtct gcaatccgcc tgccaaagtc aaaaaagtac
    12061 ttttacctga accagaagga ccgatgattg caacaaattc ccctttatcg atactcatgg
    12121 ttgtttcttt caatgcttta actacttggt cgccatcttg gaaacttttt tctacttctt
    12181 taaattctat tgctttcatg ctcttcctcc ttcttatcca attgccttca acggatcgat
    12241 ttttacgatt gtccgaacag aaaatagtgc gcctaacaca gctactacca gcatcaacaa
    12301 gctaatgacg ccaaaaaaca gccaattgct ttggaatgga accgcatcag gtagaacaag
    12361 ggatgttccc actgttccta atagaccaag aagaatacca acaaatgata aaatgaatgt
    12421 ttgtgccact acagaacgcg caataaatcc tcctgtgatc ccttgtgctt tcattacacc
    12481 aaagatatta atcttttgca tcgtcagtac ataaatgaaa atcccgatca caatagccgc
    12541 aataacgatc aaaaatccga tcataaatcc aaaagtcaat acttgcgcat tatatccagg
    12601 taattcattg atgaatttag aaattttgat ctgttctaaa tcttcaggta aattactgat
    12661 tttacctctg acaactattg cgttaatccg tgcattttca cttgtatctt ctttttcgaa
    12721 gcggatctct tggtaagcat tgatggttgt atagagaact ggtgagacat tgaatttggc
    12781 atgatccgta aagccagtta gtttaaacgt cttgtcacta ccagataact taactgtatc
    12841 tcctacggct agaccatatt cttctttcaa actaatatcg cctactgcct catcatcgtt
    12901 atcaaaagct tttccttcaa caagattggg catgatgaat tgatttttat cgataccgaa
    12961 gaagctaaca ttgatttttc cttcttctgt actgtcttta ctagtcacga ctccaggtgt
    13021 ctgggcaaga tacgccactt tcccaccttc gacttcttct gctatcttct tagtaatcat
    13081 ggacatccct agatttgaat ttgcatcttt cgaaagcaca atcgaatctg cctcccactt
    13141 gtcgactgct gttcgattat cttgagctaa gccataagct aacccagtca aaaaaaatac
    13201 caggtaggcg atcaaaaaca tcacccctgc aactaacgca taacgtaact ttgaatgcat
    13261 aatttcattt aatgctaaaa acatttaact cctcctcctg aaataatcat
//
LOCUS       DAZKCN010000068        13285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000068 DAZKCN010000000
VERSION     DAZKCN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.069"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1685
                     /locus_tag="ACG8K3_001898"
     CDS             90..1685
                     /locus_tag="ACG8K3_001898"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP9145755.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            1675..2874
                     /locus_tag="ACG8K3_001899"
     CDS             1675..2874
                     /locus_tag="ACG8K3_001899"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9145756.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            2887..6036
                     /locus_tag="ACG8K3_001900"
     CDS             2887..6036
                     /locus_tag="ACG8K3_001900"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP9145757.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7971..8636)
                     /locus_tag="ACG8K3_001901"
     CDS             complement(7971..8636)
                     /locus_tag="ACG8K3_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145758.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8762..10798
                     /locus_tag="ACG8K3_001902"
     CDS             8762..10798
                     /locus_tag="ACG8K3_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFP9145759.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10802..11440
                     /locus_tag="ACG8K3_001903"
     CDS             10802..11440
                     /locus_tag="ACG8K3_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145760.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11468..11896
                     /locus_tag="ACG8K3_001904"
     CDS             11468..11896
                     /locus_tag="ACG8K3_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145761.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12353..13264
                     /locus_tag="ACG8K3_001905"
     CDS             12353..13264
                     /locus_tag="ACG8K3_001905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9145762.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         4621 a         2080 c         2596 g         3988 t
ORIGIN      
        1 tttctttttt ttttgctttt tataaaaggg ggtatgctaa aataagaggg tatatagcaa
       61 ctttaaataa gagataggag tgtatctttt tgggtgcaga attaaatcaa aaattattta
      121 gtgcggcaga taatctccgt agtaaaatgg atgcgtcaga atataaaaat tatttattgg
      181 gactgatttt ctataaatat ttatccgatc gattgttgga acaagtggtt cttttagcag
      241 atgaatcctt agaggaatat gacacagtaa gcaaacaaac aatgttgtat cgtgaattac
      301 tttcagatga agaatcaaaa gaagatctga ttgccacaat cgttgatatt ttgggatatg
      361 caattgcacc agagtatctt ttcaacgtct tagcggatca agcaaaacaa gcaacgttcc
      421 aattgaacga tctaaacaaa gcctttgtgc aattggcatc tacctacaat caatttaatg
      481 ggttatttga tgatgtggat ctccaatcaa aaaaactagg aacagatgag caacaacgaa
      541 atgtgacgat taccgaagtc atcaagaaat tgaatgatgt agatgtttta gaacacgatg
      601 gggacgtgat cggggatgcg tatgagtttc tgatcagtca atttgcttca gaagcaggga
      661 agaaagccgg agaattttac acacctcata tggtttctga tatgatggcg caaatcgtaa
      721 cgctcgatca aaaggaacga cgattcttta gtgtttttga tccaacgatg ggttcaggtt
      781 cattgatgtt aaacgtacgt aactacttaa cacacccaga taacgttaaa tatcatgggc
      841 aagaactaaa tacaacgaca tacaatctag caaaaatgaa cttgatttta catggtgtcg
      901 acgccgaaga aatgaatctc cgcaatgggg atacgttgaa taaggactgg ccaacagatg
      961 aaccttacac atttgatgca gtagtaatga acccacctta ttcggctaat tggtcagcag
     1021 atacgacttt cttagatgat tctcggttta atcgttatgg gaaattagcg ccaaaatcca
     1081 aagcagattt tgcgtttctt cttcatgggt tctatcattt gaaggaaaca ggtaccatgg
     1141 ccatcgtctt accacacgga gttttattcc gtggcgctgc agaaggggtc atccgtcaaa
     1201 aattactaga agatggcagt atttatgctg tgatcggtat gcctgcgaac ttgttctttg
     1261 ggacatcgat tccaacgacg gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat
     1321 tattcattga tgctagtcgt gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag
     1381 aaaacattca aaaaatcctc gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc
     1441 atttggcaac ctttgatgaa atcaaagaaa atgactataa cttaaatatc ccacgctatg
     1501 tagatacttt cgaagaagaa gaaccaattg atatggttca tgtcggaaat gacatcaaga
     1561 agatacgaca agaacagcaa gtgctagaaa aagaactttt agaagctatc tcttccctgc
     1621 aaacaacacc tgaaaacgaa atgtggttac aaggtgcgct ggaggtgttc aaacatgagc
     1681 aatgatacac gaccagaaat ccgtttccca ggatttacgg aagattggga agagcgtaag
     1741 ttaattgatt tagttgttag acttaataaa agtacaaact ccagtagact tccaaaagtt
     1801 gaatttgaag atatcgtggc aggagaaggt agactgaata aagatgtgag ccagaaattt
     1861 gataatagaa aagggattga atttttacca aatgatattc tatatggaaa gttacgtcca
     1921 tatttaaaaa attggctcaa ggcaactttt acaggcgtag cgattgggga tttctgggtt
     1981 tttagagcta agaagagcga ctcggacttt atttattctc ttattcaagc tgatagatat
     2041 caaaaggctg caaatgatac atctggaaca aaaatgcctc gttcagactg gaaaaaagtt
     2101 tcagatacag tattctatat tcccaatgat ttaaaagaac aacaaaaaat cggcatattc
     2161 ttcaaaaaac tcgacgacac tatcgctctt catcaacgta agttagattt gctgaaggaa
     2221 acgaaaaaag gattcttaca aaaaatgttc cctgaaaacg gagcaaaagt gccagaaatc
     2281 cggtttcctg gttttacgga agattgggaa gagcgtaagt tgggagaatt aggtaaaact
     2341 caatcgggaa ttggatttcc tgatgccgag caaggtggtt ctgaaggtac tccatttttt
     2401 aaagtttccg acatgaataa cattggtaat gagtatgaaa tgagaaatgc aaatcattat
     2461 gtatctaatg agcaaattga gcgtaaaaaa tggaagccaa taaaagatgt gccagcgatt
     2521 atatttgcta aagttggcgc agcaataatg cttaatagaa aacgtttggt cacaagccct
     2581 ttcttaatag ataacaatac catggcttat ttatttaata atacttggga tatctatttt
     2641 ggtaaaatat tgtttgaaac tataaatttg ccaagatact cccaagtagg ggcattgcct
     2701 tcttataaca gttctgatat tgagaatatc agtgttaagg ttcctgtaaa ggatgaacaa
     2761 caaaaaatcg gtactttctt caaacagcta gatgatacga tcgctcttca tcaacgtaag
     2821 ttagatttac taaaagaaac gaaaaaaagc ttcttacaaa agatgtttgt ctaggaggga
     2881 agaagaatga aaattagtca ccgtgaggaa gcagaagtcg aagaacagtt gattcgtgta
     2941 cttggagaag ggcataatca gtggacgtat cgtccggatc taaaatcaga agaagacctt
     3001 tgggtcaatt tacgccaaaa aattatctca aataatcaag cagaactgaa tgattttcct
     3061 ttgaccgata aagaatttga aacgatcaaa acggaattac tgctgcgaac gaaaacccca
     3121 tttgatgctg cgaaatggtt aaaaggtgaa aatgggatgg ctcggatcac gattgaacgg
     3181 gaagattcgc aattgggttc tgtctcactt atcctttatt ccaatcaaga cattggtgga
     3241 ggaatctcta cttatgaagt ggtccatcaa attgcaaaac gtggaagtaa catagaagca
     3301 cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag
     3361 ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat
     3421 gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
     3481 aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt
     3541 ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa
     3601 caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa
     3661 gatcaagata ataaaaccct catggtgtta catccttatc aagtacatgc gattcaagcc
     3721 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
     3781 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
     3841 acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga gtttacaaaa
     3901 tttgcttcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa tagcttgatc
     3961 gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc caatgcaaat
     4021 gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacaa
     4081 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat cgtgtttgta
     4141 gtagatgagt gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa aaaaatgttt
     4201 ccgaaatcca cttggtttgg ctttacaggt acaccgattt ttcctgagaa tcggaaacaa
     4261 gccaaagggc aattggcacg tacaacccat gatcagtatg gagaggtctt gcatacctat
     4321 accattaaga atgcgctaga agatggctct gtcttaggat ttcaagtaga gcatgaaaac
     4381 accgtggagc cgacttcact agagaataaa atctatcgga aattaaaaga agtagaaaca
     4441 tatgcagagt attcatcaga acagatcaat cgtatgattg atcaaatgga acctgtcaaa
     4501 aaagaaagtt atttggaccc agctgttttt gaagcagatg aacatatcca aaaagtcatt
     4561 cataaaatgt tccgtccaga taatgcctat acaaaatttg attttcgaaa tggcaggcca
     4621 acgaaatctg caatcttaac cacgagttcc attgacatgg ccaaaaagta ttatcgggcc
     4681 atcaaagaga tgacgaaaga gcctgattgg ttaacaaaag agtttcccaa tcagctcatt
     4741 cgtgaaggac gaacgatgga ggatcctgat tttccacgaa tcgctattac ctattcatta
     4801 gaagaaaata gccgagattc aagtgcccaa caagaagaaa tgcaaaaaat tattgaggaa
     4861 tataatcagt attatgatac ttcctggtca ttagcagaca ttgagcgtta taacggagac
     4921 atcaataatc gtttggcacg aaaaagagcg gaattcaaac aatttgggaa acaaatcgat
     4981 ttagcgattg tagtggatcg tctattaaca ggttttgacg cccctacgat tcaaacccta
     5041 tttgtggatc gtaatctaga gtatgcagga ttgatccaag ctttttcacg tacgaatcgt
     5101 acgtatcctg ataagacaaa aggattgatt gtgacgtttc gtaagccagc gacaatggag
     5161 aagaatgtcg aggatgcgac aaaactgtat tcaaaagcaa aagaagaatc aggtttgatt
     5221 tatccaagtt atcaggaatc caaaaaaaga ttcaaacaag catatggtaa gttaaaagag
     5281 gtttcatcca tggaatccat tgatgaacat acgccacttg aaatccgtat cgagtacgtc
     5341 aaagcatttc aagaattgaa tcgtgcttat gaatcgttag tgacttatga cgattacaat
     5401 gatgacatgg tgaaatctcc aactttgaac aatcaaattc aacttttgga agaacaaatt
     5461 ggcgtgtacg agaccgtgaa gggttcatta atagaggaag aaccagaaaa agaaggagaa
     5521 gatttttcaa cgatcgaatt ttatagtgaa aataccacta agttgtatga tattgattca
     5581 acttatattg atcaattact aggaacttat gctgcgaata gttcagatgc acgagaagaa
     5641 attgaaaaag ctttggctaa attgaataaa acagaaggag tcaaagaagt ttaccgacaa
     5701 attttaaatg catttgatgt ggggacttta gatagtaatg aagatatctt tgtcattaaa
     5761 cgtcgctatt ttacacaagc gagtgatgat ttgattcatg tcttttccaa tgagtggttt
     5821 gtttctgaga aagaattaca tgcttctaat ctgcaatatc taccaggtga agatccaatt
     5881 cctaatatga aagcaattat caacagtaag gattacgaag gatataaagc caaacatcca
     5941 gaagcgaagc catttaaata cccacaagaa atgaagcgag catggcgtaa aatgttagat
     6001 gatgagttga ttccacttga gaatgaattg aggtaaacga gaagagttaa gaaaattgta
     6061 cgattcaaag atggtagtca agtggaagtg tagaaaatag ggatagatgt cttttatttg
     6121 ttcacatttt ataacgaagt atttaagaca gctattgaat aatgtttcct ccaccttttt
     6181 cattcattta ccccaccccg ttgttatatg accgtgtttt gagaggaacc aataaaaagc
     6241 ataggctaac taggggttcc tttttctttt gtttttttgg aatccgctta agtgaaatgt
     6301 atctgaaatg aaacggagat aaagtgattt tttgctataa tgacaaggtt ttagctgttt
     6361 cagataccat gtaacccaaa atagtttatt gagaaaatag gagagtattt gatactaact
     6421 agtaggaaaa aatgagataa aaattaaatg gaatcatttc aggtaatctc aaataaaaaa
     6481 atatacagtc ctgtggcact ttacctatgg gactgtatat tttttactct cagcgggctt
     6541 gttttgactc cgtgtctgac tccgtcgtat tgattttaaa tgatattcga tgaccattta
     6601 acaggtcatt tttgaaattt ttgtctctat atgactcagt ttgataatta ttgatagatc
     6661 aatttgggtc tatatcgaac aagggaataa tccaataaaa aatgatttag aatatcaaaa
     6721 taacaaaaaa gaggactacc tgtattcgtc ctcttttttg accagatttt gaccagtcta
     6781 atttacattc taatatattc atatgagcta attttttgat taaatggtaa gtttttcctt
     6841 gtttgtttta gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg tctgtggggt
     6901 aatacttaaa taaaggctag gaacctgata acatcaaggt tcctagcctt tttataataa
     6961 aatgaagcga aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt
     7021 tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga
     7081 atttaagaca aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat
     7141 aaactaggta actaactgta acaagctaat tttaagagat tcttattaga agacgtttag
     7201 gaatataact aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt
     7261 tcgcgtcttt gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg
     7321 aagaggggca aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg
     7381 tgtgattcct ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac
     7441 aatttttaca atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt
     7501 ttatttgttt cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg
     7561 agtactcttg gataaactac cagcgattat catatttatt tatgttactg taagccatca
     7621 tataaaagta aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta
     7681 tgtgcagctt gactacgtac caatctatat tacttactag attggtcgtt aatctattcg
     7741 gatcgaatga gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat
     7801 cattgggagg ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac
     7861 ttttttattt aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt
     7921 ggataagatc atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta
     7981 tctggctcct gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg
     8041 taatctgagg gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg
     8101 tgtttgatta cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat
     8161 gtaccatggc caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct
     8221 acagggagtg tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt
     8281 tcatttttgt attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag
     8341 aaagatagtg tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca
     8401 agaggttcat ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa
     8461 tcaatcggca gacctaaagt atgatgtaaa agtgacagca tttcaaagtc tggcattgta
     8521 cgagaatttt cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct
     8581 ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc
     8641 ttctcctttc gattctacag tatatagatt tattctacat gacctataca cttgatagat
     8701 acgttttttt gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt
     8761 ggtgataatc aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag
     8821 tggttttgcc ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca
     8881 tcaaacagat acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata
     8941 tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa
     9001 aaaaacagta aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa
     9061 gcttgaaatg acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta
     9121 ttctaatacc tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga
     9181 aggtgaattt ttcttgatga cgagagaaaa agaagcctat gaacgggtca tttcactatt
     9241 cactgatcga ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt
     9301 tgaaaatagc tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta
     9361 tttaactatt ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa
     9421 taaagaaatc agtttattgt tgaataatga cttttcatta catcaagtat tgtggtgttt
     9481 attggggaaa aagaaaatca ttattacatt cataattgca ggaatcattg gtggttttat
     9541 ttttatgtat tataaggagc aatttcttta tttgttccag atgtacgttt tcttgttcag
     9601 cggaatatat ggtatctctt tgatttctct tttattagtt gaaaaaatcg cttcgttaag
     9661 agaagcgttc aactatatcc aatacattct ttttacattt ggattgttgt tttctcttct
     9721 tttgaccgtt gccaatgtcg aattagcaac tatcgttttt tcttcttcac agttagctgt
     9781 ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat catgtatttt atccagtaac
     9841 catcgggaaa aaccacgtgg attttattta ttccaatcgt tttgcatcag cagcagatca
     9901 ggaattatat gaaactttga ataaaaacgg aagtatatta gtcaatgtat cagattactt
     9961 gaatgaagaa aatgaacagt ttggcagagg gataaaaata aatctgaatt atcttaagaa
    10021 atttcctttg attgatgtct ctggaaggtt gatccaaata accgaaaaag aaacgcaccc
    10081 tgttatattg atccctgaac gatatagaca tacagattta aaaaatgacc tggcgcaaat
    10141 aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta taaaaaacaa
    10201 tcaacccatt tatacattta ttccttctat cccctggatc gaacattatc cggttgttga
    10261 aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg gagaaatcta
    10321 tccaccgtta aagatcaaga taggctcttt gtcgaaagaa aggttatttg agctgattga
    10381 agaaagtcag ttacgtgata acttgcagtt atcatttccg tatactcaga ctgaagagat
    10441 agctgtcaaa gtgttaagca gatcatttca ttacttgata cagatatttt tactcacttt
    10501 cttttctttc ttcttgttga gctatgtcat gttatgcata tattttgtct acaattgtcg
    10561 gaaaatcgct atctttcgtt caagtggtta ttcgctcttt gaaacgtata aagatttctt
    10621 catgatgaac ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc ttatagaaag
    10681 agagcctaaa tatcttttta atattttttt ctttagcttc attgatttta ttctttcagt
    10741 tgtttttatt ttgtctacag aaaagaaaag tcagttattg ctgatgaatg gaggttaaca
    10801 attggagccg ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca aaacgattct
    10861 tcatttggat cgtttcactg cggatgctgg ggatcttgtg actgttgttg gagaaagtgg
    10921 agcaggtaaa agtacgttat tgaatatatt aggattgaat gatcagttca gttcaggaca
    10981 attctatttg ttcgatcaag agacgaaaca attaactggg agagagaagc gcttgataaa
    11041 aagaaatacc atatcttttc tttttcaaga ttttgggttg attgaatatg aaacaatcga
    11101 ttttaattta gatattggat tgaagttttc gaatttaaat gtttcagata agcgaacagc
    11161 aaagtatgag gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc ctatctacac
    11221 attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga agaatagcaa
    11281 aatcatttta gccgatgaac cgactgggtc attggataaa agaaataagg ataaagtagt
    11341 tgaaatctta tcgcaacaag cttcccaagg tacgttagtt atcgtagtca cgcatgatcc
    11401 atatctgacc aaaataggaa acaagaagat catgctttaa aaatcaaaaa ggaaaggatg
    11461 tcgttttatg aaaaaagcag ttattatttg tgcactaggg ataggttttg ttggaggaaa
    11521 cgctatgtca tctgaagtgg aagcaaattc agttcacggt gaaatctatt acaaatcttt
    11581 aaagccagga gagattcatt ttgatgctaa aggtaatatt attaagtcaa atttaccagt
    11641 agaccgggaa atttctagaa cagcaattaa tcatagctct ggaagatggg tttattatag
    11701 tgatacaact tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg tctataatcg
    11761 tcacggtgca aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa caaaaggagt
    11821 atgggcctat gcaagtgcga caggaaatag aactgacaca ttctcatgtt ggtacaatcc
    11881 aaccggttgg tattaagtat ccaaaagttt atataaaata gaaaaaacat ataatgaaac
    11941 agcgttaaaa gttatttttg gtagttttaa tatattcatt tttagttgta taaaaatata
    12001 tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt ctgatttgaa
    12061 tatcccagca gcggaagaag aaaaacaaaa tcgataacat ttgttctaag aattagttac
    12121 gttaaatacg gaacgcaaga tagacttgaa aaacggtaac aaaagaagta gtatattaag
    12181 gtgtgatgtg ctacttgaca tttattctgc tgagcgaact ttctggattg tgtagcctta
    12241 tatctattca ggacgtatcg agcagattag taaaggtgtt cttgatgatt ttcatcttga
    12301 acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat aaatgaaaaa
    12361 aagattttta gcacttgcca tcgtactagg cactggctta ttgtcaggct gtacgaatgc
    12421 cggtgaaaaa acagccgtta gttataaagg cggtacaatc agcgaacaag aagtaatgga
    12481 tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg tttatcaagt
    12541 atttgaagat aaatatggag atgacgtatc aaccaaagaa atcgattctc aatatgatca
    12601 aacaaagaaa caattgggtg attcatttga ttcacaattg aaatctgcag gttacacaga
    12661 acaaacattc aaagacagta tcaaacaaag tctagccttt caagaaggtc taaagaaaca
    12721 tatcaaattg accgatgaag atttaaaaac ggcttgggaa tctttccatc cagaagtgga
    12781 agctcaaatt atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga aagctgctga
    12841 taagggagac gatttctcta aattagccaa agacaagtca actgatacta cgacaaaaga
    12901 agatggcgga aaagtgaaat ttgattccac tactacaacg gttccggcag aagtcaaaga
    12961 agcagccttt aagttgaaag atggtcaagt ttctgatgtg atcacttcta ccaatgcttc
    13021 aacttatact acggaatatt atgtggttaa aatggtgaaa aaccaaaaca aaggcaacga
    13081 catggataaa tacaaaaaag aattgaaaga aattgcaaca gacacaaaat taagtgacag
    13141 taccttccag aataaagtaa ttggcgaagt attgaaagac gctaatgtaa aaatcaaaga
    13201 caaagacttt gaaaatgttt tgagtacatt cacttcagat tctagtacag caagtagtaa
    13261 ataagcggta taaataaaaa atatg
//
LOCUS       DAZKCN010000069        13204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000069 DAZKCN010000000
VERSION     DAZKCN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.070"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1240)
                     /locus_tag="ACG8K3_001906"
     CDS             complement(<1..1240)
                     /locus_tag="ACG8K3_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9145763.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILK"
     gene            complement(1237..3426)
                     /locus_tag="ACG8K3_001907"
     CDS             complement(1237..3426)
                     /locus_tag="ACG8K3_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9145764.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3560..4750)
                     /locus_tag="ACG8K3_001908"
     CDS             complement(3560..4750)
                     /locus_tag="ACG8K3_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFP9145765.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5622..7253)
                     /locus_tag="ACG8K3_001909"
     CDS             complement(5622..7253)
                     /locus_tag="ACG8K3_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP9145766.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7267..8940)
                     /locus_tag="ACG8K3_001910"
     CDS             complement(7267..8940)
                     /locus_tag="ACG8K3_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP9145767.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8953..9270)
                     /locus_tag="ACG8K3_001911"
     CDS             complement(8953..9270)
                     /locus_tag="ACG8K3_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9145768.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9295..10695)
                     /gene="lacG"
                     /locus_tag="ACG8K3_001912"
     CDS             complement(9295..10695)
                     /gene="lacG"
                     /locus_tag="ACG8K3_001912"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP9145769.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10932..11717
                     /locus_tag="ACG8K3_001913"
     CDS             10932..11717
                     /locus_tag="ACG8K3_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9145770.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11720..12412
                     /locus_tag="ACG8K3_001914"
     CDS             11720..12412
                     /locus_tag="ACG8K3_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9145771.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(12686..13057)
                     /locus_tag="ACG8K3_001915"
     CDS             complement(12686..13057)
                     /locus_tag="ACG8K3_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP9145772.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         4046 a         2704 c         2174 g         4280 t
ORIGIN      
        1 tttttaatat aattaaaata tcgtttgcaa ttagctttct tcttcgaacc ttttcacctg
       61 attcagtaaa gtacaaaatt gctaacttga aaaataaact atgattattt accttaattg
      121 gtgatatatt tatagcatca aaattgcaat acggtaattc tttctttaaa tactcaccca
      181 gtgaaaacag aggatagtgt tgaggagcaa gtacacaaat atctttttca tgaatgccgc
      241 tttcaatctt ttcctttatt atatttttta ttatgttggg taaatcctcc ttagtaactt
      301 tgcaatcata acttataaaa ccacggtcgt ttttattatt ccctctgctt tcaatctcgt
      361 aaggacaact ctgaaaatta gagtagaagt ctactaatct ctgattagac ctgtaacagc
      421 catgtagcgt ctttgattga aaagtgtgac tagttagcat ttctagttct tcaactgatt
      481 tagctatacc atctaatgat ccataaattg cttggtcaat atctccaaca aacattgact
      541 gaatttgagg gttgttttga agtaatttag ctattacatt gtattgtaat tctctagtat
      601 cttgaaattc atccacataa atcgccctta tattactagc aatattacgt gcaacgttac
      661 tgtttacagt taaaatttta tacgctacta gtaaaatcat atcaaaatca atttctttat
      721 ttttttccaa catagtgtga tacatttcag aaacttgagt atactgaact tcattaattt
      781 taaaatcatg agaaagtgac aaatcaattt tgttcagttc atacgttttg agatttaaat
      841 tcagcatatt attaatttgg ctgacgtaag ctctttgagt ataatcatca ataattgtaa
      901 atccttttga tactcttgag agattcattt taaaaggata aataataaat tcaagacaaa
      961 actgatgtat tgtccccacc caaatattaa tatcatcatc tatactaagt aaacttaggc
     1021 gttccttaat ttcttcagca gctctattag tgtatgtgat agcaattatt ctttttagtg
     1081 atccatcgtg tttaattgct tggtaagcaa ttttccttgt aagtaccctt gttttcccac
     1141 tcccaggaca tgcatttaca taaaggttat cttcactttc cacaacatct ttttgttcta
     1201 aatttaaatc atttacgatt ttttcgaaga tatttgtcat tttgaaacct cagcataaag
     1261 tatttttgtg taattttcta tctgattgat tagatacaca acatcctcat ctttaccaaa
     1321 gtactctaat aaagaaatta ttgctatatt caaatcagct gtatcacttg tttttaaact
     1381 agctgataaa gctgacaatt ctgtagtttc ctcgtacaag tcgattgtat ggagtagaat
     1441 ttttttataa attgagagat ctactatttc ctgacatgca aaagcaatag cattaagaat
     1501 atacttagga attttgatta aaaagtcaat tttttcacct aactcaattg caaaccaccc
     1561 cttaccaaca tgattggcta gttttaacat cgctaaattt ctttctaaat attgttcact
     1621 ttgtatttct tctttataag cagttattgt ttttttatct ttgaatgcgt catctataat
     1681 tggactgaca taagttagaa tattttcttt attcacatct aaaaactcaa tttcaaatgt
     1741 tgtattagca taaaaacaat taacccaatt gttttcatta aaaagggagg ataatttttc
     1801 ttttcttgct tgacctttct cttgtgcagt gcttttatac aatcgatgtt ctgatgggat
     1861 tgcctgttca tctaaatcag ttacaatagc acactttctc tgtattctat catcgtcaaa
     1921 caagctagcg atatattcaa acgaagtact accaacatta atcaaaccta ttccaagttc
     1981 gtctaagttt ataccaaata atttttttac tatttgagga ataagtattt cttcagcatc
     2041 tccttcaact aagataacac ctttagaaaa aagcaatcca tttcttttaa catctaaata
     2101 tctctctact gcgtttatta gatttagatt gttctttttt aacttttctt tagcaaatga
     2161 gttcagatta tttgctggat tcataacgat agatttgcca ttaaacgatt ttaatacatt
     2221 catccgagat atttcagaca cttcagctaa atgtacagaa tgtgtggtca tgagaatctg
     2281 agtatagttt ttttgaagat ttaatccttc gaacagagtt ttttgaatat gatgatgaat
     2341 atgagcttct ggttcttcaa tcaacataat attaagtagt tctcttgaac gacacgcttc
     2401 aaattcgact attttcaaag ctaaataaat catatttaga tgtcctaacc ctagtaagtc
     2461 taagtcgata ttttgttctg gcttcattga aagaaatttt gctaaagaac ccatatcatc
     2521 tgacatagtt gaggaaagaa gaagattagg agaatacact gaaccaatta tgctatttag
     2581 ttgccgatta atattttcgc caattttcct aatttcagga acactggtaa tagtctcatt
     2641 taattgctgt ataattgttt ttaatgactc aacattatct gatgatattt ttgattcaat
     2701 tgtttccatg attcttttga ctgggtttcg attgtttttc atttctctaa gaacatctcg
     2761 aagagcgtca acataaacaa ccgaaatatg cttgaaaata tctgacatgt caattcttga
     2821 gcccaacaga gcttcatcgt cttcttcagg attactagct atattgtcat tgagtcttcc
     2881 taccaattta tcataatttt cattgataca gaaatttaga tttgatttag atgtgtagta
     2941 aaattcatag tcggatagac gaatagaatt tctaaattcg ttaaattgat gagagtctaa
     3001 ttcagtggaa atatcaaata attgctttct aatagctttg ttaggtctaa taaatagaga
     3061 aatactacct acacctttat ctgcatttga aatcaattct tctaagattg tttgactttc
     3121 agaatttaaa gaaatggaag cacaaatctc attgtcaaat tcttcttcgc tgattccttc
     3181 aaatgtcgca gagataataa tccaatgccc ttgccaccca ttttgaattc catgaaaaaa
     3241 atctgattct tttagtgttt tactactata gtaataacta tcatctaata agattctcaa
     3301 tgcagtaaga gcattggttt tccctgaatc attctcacct ataatagtat ttgtctcatt
     3361 atcgaatcga aagatttcgg attcataatt tctaaaattt cgaatttgaa gagtagatat
     3421 aaacataagc agatctccta actatttaat taatatgatt atatagtaag tttatcataa
     3481 attatgttag taaaagttct cattataagg gagcgtcaat aattttgtgt aaatgattct
     3541 tccctactgc agattgtttc taattctcaa tcagttggct tagcatttgt tcgagctcgc
     3601 cttcggcctg ttggaaacct ttatgactgc gacctaggaa tttttggttg taagtatcaa
     3661 aagacgagac taagaaacgt tccatcgact cttcattttg gaactgttcc ttgcggtggc
     3721 tgtatttctt gatttgttta ttaaaggatt caatcaagtt cgtagaataa atactcttgc
     3781 gtatggccaa ggggaaatca tagaaagtga gcaagtgatc gttcgaaaga atcgattcaa
     3841 ccacttttgg atagctggtt ttccattttt ccgcaaaagt accacgtgct tccaacgcca
     3901 cctctttaga tgaggcttga tacaccattt taaaatcatc acagacttcc ttacgatcat
     3961 cgacacgcac tttatgactg atattacggg aaacgtgtac acaacaatgt tgaaaacgag
     4021 ctttgggata gaaccgacta atcgctccta ccatcccctt taagccatcc gtgatgaaca
     4081 ggaggacatt tttcaaaccg cgctcttgaa ggtccaataa aatttcctcc caaatcgtga
     4141 tggattcagt cggtgcaatc gcatagctca atacttcctt tgatccgtcc gggcgaatgc
     4201 caactgcgat atgaatagct tccttggcga cggttttccg ctttaacgga atatacgttg
     4261 cgtccatata aatagcagca taacggtcat gaagctcccg cccattaaac gccgttacct
     4321 cttctgtaaa tgattttgtt atattggaca tggtttgggg cgtgtagtaa tgcccataca
     4381 ttttctcaat caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga
     4441 cggtctcctc taacgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact
     4501 caccattgcg gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac
     4561 ggtcatagga gccgttacga gaattacccg tgttaaaacc aatgcgatca tacttttcgt
     4621 aatctaggaa agccgttaac tccgttttga gaagcgtatt gactgctttc tccaaatgga
     4681 cacggaataa ttcattcaaa tcgcctttgt tcgctagagt ctttagaatt tctgtagtaa
     4741 aatcgttcat agggaagtcc tctcttctgt gaatgtgttg tggtaactct attctacaga
     4801 agggacttcc ttttttctat ttacacaaaa tattttacac tctccattat aacaattggg
     4861 ttaaaaagaa aatagatagt attctacctc atatttattt gaaagtaata tataaaagac
     4921 gttcaaattc gaacgcctct cgttaaccaa atattggatt cagaatctag ttttcatata
     4981 taaacagagt atgattaggt gcaaaaattg cacttttatt gcacttttat ttttatttat
     5041 agtgttttac tgttctgtat attgttttaa ctcccttgat agtatcgcat tctgttttat
     5101 acactttacg ttgaaaacag agtgggaata aagagccaac ataatatgga gggggaatcc
     5161 ctataaatca acattcttca atagctaaaa tgcttgattt tcattaaaaa taagatggtt
     5221 ccctaccaaa agagactata atataagtta atttgtttca ttcttatcat aaaaagaagt
     5281 tctcgaaaaa cgaactgttt tccgagaact tttttctatc catctaagtg ctcaaatcat
     5341 attcacgacg gaattctaag gttacccttt cttaaacaaa ctttatgtta tctgcacaac
     5401 tttttgcaat tttttcattg cttcttacga aatcgttgaa ctctctttag cataatacaa
     5461 agtgtagatg aagtctggtg ctttactcgt tttggtaaaa atgactggaa atcgtttctg
     5521 gttatttttg gcaaaggcga ggtgcaggtt cgattcctgc cttctacgct aacgatgtgg
     5581 ctgaagggaa cctccgatct tgaatcgaaa cgatccgcag tctattgttt caccaatgaa
     5641 acaaaatcaa cgttcgtcca agcatttcct gaagccttgc tataaacccc aatttcacaa
     5701 acacctgttg taattggaat gtctttgatt gtaatttttg tccattcact tgttttaaca
     5761 accgcagtat ttagttccgc tccaccataa tttttgatat ataactgatt agtatcaaac
     5821 tctcccccag acattacata ggcactcaat gtgtaagttc ctttgcttat gccactaaca
     5881 ttttgataag ctgatgcctc gtatgcttga tcctgccaaa aagttagttt gtagtcccca
     5941 aaaaaagtgc ccgcttccgt tttgatcgta cccttagcgg ttccttgttg aaaccaggtt
     6001 tgccaattgg aaggactggt cgttggtcca tcgctttcga aacttgcatt tttaactaga
     6061 tttatttgcg cccctgcttg tttaaacgca cgcaagcttg gcaaggcgtt tccagcaaaa
     6121 tcaaacatcg cttggttgtc ccattcgttt cccatagtgg attgaacact gagataattc
     6181 atacctgctt gtgttgccca actcacattt ccgttgtacc aaagaggttc ccaatagaag
     6241 aagcccgccc cgcgattatt cggtactgcc tgaatccgat cggcaagatc cagcaaatac
     6301 tctgcttgtc ctgtcggtgt tggttcatat ccccccgcag ctgcttcggc ttcaccaaaa
     6361 gcatttggca tagtatcggc attcgctgtt gtatgcccat aagctgtttc aactacgatc
     6421 acttctttac cataccgttg gctgatcgca ttcatattgg tttgaagttc attcattgaa
     6481 ccgtgccaat acggataata cgacactcca attaaatcaa atgacacacc tcgactcgtg
     6541 atttcatcaa accaccaagt aaatgctcct gtatcgccac catcagctaa gtggagcatt
     6601 actggagttg ttttaggttg agtgtctcga acagcttgga taccagactt caaaaaagca
     6661 gccaatcgat caaattctcc cccgccttct ccccaagact tgccataagg ccagagcatt
     6721 ccgctattca gttcattgcc gatctgaacc atatcaggat aaacgtccag ctgcttcatt
     6781 tccgacagaa cgttctttgt atagtcgtac aatgccgtat tcatttcttc aaaggtcaga
     6841 ccttgccatg ctttaggaag attctgtttc ccaggatcaa cccaaaaatc actgtagtga
     6901 aaatccagca gcaccttaag tcccagattt tttgctcgtt ttgcaaggtc aatcgtcgtt
     6961 tccagatcat ttgtccctgc tccataactt tctccattac tattataagg atcctgccac
     7021 aaacgcagtc gaacatagtt gacaccatta ttttttaaaa tctctaaggc atcttttgaa
     7081 atatcgtctt cataatattt tgcaccagat ttttccatat ctgacaggat cgaaatatct
     7141 gctccgcgaa taaattcgct ggcagatgct tcaacagttt ttgaaacagc ccccaaagcc
     7201 cctaacacag ctgcaaataa aacagcttta cgcaaccaaa ttcccatttt cattatggct
     7261 cctcctttat tgaaattggt tcagaataaa ctgaacagct ttttcaggat ctctcgttaa
     7321 tccgatatat tctgctcctt ttgtcgcaac cagcttgatt cctaagcgat cggtgtcttc
     7381 tttgatatca tcatagtaag aattgacttg cggtgctaag accaccatat caaaatcctg
     7441 cagcatttcg taatgagaac cataagcacc cgccattgca ctgatcggtg ctttcgattc
     7501 agatgcacct ttttttaaag cattagctaa catcgcactt gtccctgcgc cagcacaaag
     7561 taccaacaca ttgatttctt tatttgcatc gctgataact tgactcgctg ccacaggtac
     7621 ttcctcacgt acatcggcag aagcaccttc ctcggttgcg atcacctctg ctttttgtgc
     7681 ttctttctct actaagacag catcgtatgc tttgcaaaat ggtagataga tcaggaagtc
     7741 aacgacgatt agaaccgctg ctagaacaaa agctaagata cccatgcccg ttcctagaac
     7801 caaaccgatc ggtgctggag ttgcccaagg caaaacgtac ataaagctgt tcatgcccaa
     7861 tacatcgaca aaaaacttga acaggcaaac gtttacaaca ggtgttaaca aaaatggaat
     7921 aaaaaaataa gggttcaaaa tcatgggtgc cgcaaatagc aatggttcgt tgacagcgaa
     7981 acttactgga ataaaggaag ctttcccaac tgccttcaac tgcttagact tagcaaagag
     8041 taggaagata aaaggaacaa ccaatgttgc cccagttcct cccatagttc ctacaaaatt
     8101 ccctaaaccc accgtcagta ctttagaagc ttgttcaccg gaattgaaca attgcagatt
     8161 ggtttccaca ttggcataga taattgccgc aattgccggc tccacgatcg acggtccatg
     8221 aacaccgaca aaccaaaaca gcgccatcgc tccccagata atagcgattc ctaagtaccc
     8281 gtcagctgct gtaaatagcg gctgcagcac cgtaatgatt gcttctgcaa atgagatttg
     8341 gaagatttgt cgaatgccta aatcaattaa aacggccgca aatactgaaa aagagaaagg
     8401 aaaaatatct cggaaggttt gcgaaatcgt tccaggaact tctttaggca ttttaatggt
     8461 aatatctttc agcacacaaa acttatataa attaactgtt aaaaatgcag cgacaaagga
     8521 agccagtagt ccctttgtcc ccatatattc cgttgaaaat ccaccttcaa tcggagagac
     8581 gcttaacaac aggaaactta ccacagaggc cagcatcaca gaggtggtgt tgattacttt
     8641 gtttttcggt aagcgacgat tcatcgaatc agccaagcag cgagcagtgg ttgctgaaac
     8701 gagtaaccct acaacaccca tcgaataatt atagactttc catagccatg tggagacaga
     8761 ttcaggccat tggtaaccaa aaatctcagg aaccgcagca atcaaaatga aaatgcttga
     8821 aaataaaata gctggcatcg ctgttaaaaa tccgtcacga acggctccta aataactatt
     8881 acgtgagatt ttttcaaaaa atggtttccc tttttcgatt tgtttaatga ttccatccat
     8941 agcgttcttc tcctatcctt gattctgtct gtacagtaca ataaagtctt gaatcagatc
     9001 tctcagcaat aaagtggtca ttaaatgatc ctgcccatgg ataaaaatga atcccatctc
     9061 catctctttc ccagatgctt cttccgctaa aatttttgtt tgcgaattat gtgcaagggt
     9121 taaattttcg tcggcttctt tcaacgccga atcaacatgt tcaaagtttc cttgtcttac
     9181 ttctttcaat aatttcaata atgtactgcg agcgtcgcct gaatatgcaa cgatctcaaa
     9241 accaagcatt tgtaattcct ctttattcat cttttcttcc tccattatca cttattaaat
     9301 aatcgttttg gtttcactga ccaacttgta ccagtaagct gattcttttg ggtaacgctt
     9361 ctgggtttca aaatcaacat aaaacaagcc ataccttttg ttatacccat ttgtccatga
     9421 gaaaagatcc ataagcgacc acaaaaaata ccccttcaca tttaccccgg ctgttatcgc
     9481 cttgcttaag gattccaaat agactctcaa atagtcgatt ctcggctgat ccataataat
     9541 gccatcttca aactgatctt tataccccat cccgttctca gtgatataga ttttgttgta
     9601 atgtgggtaa tcgcttttaa ttcgcagcaa caagtcatat agaccttctg gataaatcag
     9661 ccagtcccaa tcagttcgag gaattccttc tttataaata cgttctccga ttcccttcac
     9721 tttataaaca gaggtccctt tttcacccgt accgttatga tggattgcat tttctccatc
     9781 gtaagctttg acaaaatggc attggtagtg attgatccct aagtagtcat tacgcgtgga
     9841 tgcttttttc aattcaacga aatcttcttc aggaaaatga tagctggcct gatttgcttc
     9901 acatatctca tcaagagcag tcaaggtttc agttgaataa tagcctaaat aggtagcatc
     9961 taataagaaa cggatcgaca atgcatcgtc taaaaaggca gcatgcttat cttccggcgc
    10021 gtctgttgcc gcatatttcg tctctaatga gtgaactaca ccaatttcac ccggaagttc
    10081 gttttctttg aaatagttca ctacacgggc atgagcaacc atcatattat gaagacaggc
    10141 gacgatcttt gtaaaatcat atttgattcc tggtgggaaa acacctaaca aatattgatt
    10201 ggttgcaact ggatagattt cattaaaggt actccacacc ttgacttctt tgaattcatg
    10261 aaaacaaaaa atggcatagg aaacaaatgc ttctattgtt tctcgattca aaaaatctcc
    10321 atggtcaaac aaacgtttag gggtatcaaa atgatgcaac gtcacaaacg gcgttacgtt
    10381 tcttttctta cattcttcga acacacgatg ataaaaagcg attccttctg gatttggttt
    10441 cccagctcca tcaggaaaaa tgcggctcca agcaatggat aatcttagac catttacacc
    10501 aaaacgttca cacaattcaa tgtcctttgc atattgatga taaaaatcac ttgccggatc
    10561 gggactgaat cgcccttgtt cctctaaaaa gtcatcccaa gcaacagcgc ctttgccacc
    10621 ttccttagtg gctccttcaa cctgataggc tgctgtggct ccgccaaaaa taaaatcttc
    10681 tggtaattta agcatgtaca tctctcctca aatctataat cagttttcgc tctttctaac
    10741 tgattgcatt gtaaacgcat ccaaaacatt tgtcaacaaa aacaaacaca aataatcata
    10801 aaaaccagaa aacagcaata aaatctattg taacaaacat aaaaaccacc ctaaatgttt
    10861 acttttgttc gaattagatt gactgttgtc cgtttatatg atagattatc cttaaattag
    10921 aggtgaagac aatgttgaaa aaagaaagat tgcttactat cgtagaaatg gtcaataaaa
    10981 aaggaatcct taccgttaat gaaatcatca atcaattaga tgtttctgat atgaccgttc
    11041 gcagagattt agatgaatta gaaaaatcgg gcaagctgct gcgtgtccat ggaggggctc
    11101 aaagtctttc ctacacttta gatcaagaac tttctcacac tgaaaaagcc actctgcaga
    11161 ttcaagaaaa aaaagaaatt gctgccacag cagcctcctt gattcacgag ggagaaacga
    11221 ttttcttagg accaggtaca acaatcgaac tattggcgac gcatttactg aacaagaaaa
    11281 ttcggatcat tacaaataat tatccagttt ttgatattct tgctcaagca gatggaccag
    11341 aaattctact gattggcggt gatttccgga aaaataccgg cgcatttgtc ggaccgatca
    11401 ccaacgacaa tttaagaaga ctaaagttta caaaagcatt tatcagttca aatggcgttc
    11461 acaacgaagc gatctctact tacagtaccg aagaaggaga agcacagcag atcgctctaa
    11521 ataattcccg taagaaatat ctactcgttg ataataaaaa attcaatcga gatgattttt
    11581 atgtgtttta taatttgcat gatttcgatc tgctcattac ggatcgccag atctcgcggg
    11641 aggttcaaga gcactatgca caatatgtcg atctgcaaat tgctgattca accgaaaata
    11701 ccactatagg agcgtaacta tgaaagggat tatatttgat ttcaacggaa caatgttttt
    11761 ggactctcat ctgcatgaag ctgcttggct gcatatgata catgatcata caaaagatgg
    11821 tttatcagac gaggatattt taaaaaatat tcatggtcgg acgaatacgg agattttgaa
    11881 acatttcatt tcagagcatt tgaccgcctc tgagatcgcc gtattgtcag aagaaaaaga
    11941 aagctattat cgtatgcttt gcttgcaaaa tgaaaacgag cttcagttga caaaagggct
    12001 tgaagcgact cttgaccagc tgactgacca acagattcca ttaacgattg ccacggctac
    12061 tgtcaaagag aatgttgctt tttattttga tattttcgat ttagcccgct ggtttgactg
    12121 ggacaagatt gtctatgacg acggctcttt ccctgggaaa ccgcaaccgg atatttttct
    12181 taaagcggca gacaaactga gcttagctcc ggctgattgt cttgttatcg aagatgctta
    12241 ctcaggatta cttgctgcca aacgtgcggg gatcggaaca attattgcaa ttgatccatt
    12301 cggtaaaaac aaaacagtat ttgaacaggc acagctaaat aaagatggaa ccatcaaaga
    12361 ttttactggt ttttggacag agcacctgtc tgccacatcc gttaattctt aaacgattca
    12421 actaactacc aaagaaaagc aactgaaatg cacacctcct tttataaaaa actgccagtg
    12481 aaacgacatg aaagatacac tgctatatcc ttagtgaaat atagcagtgt atctttgact
    12541 ttttcattct tttaaccata tgacgcgttc atctctatct ataattaaca ttaaaaatag
    12601 tgttttcaca ttatttctca aaccaatctt aaaaatcatc aggagtactt ttttgatata
    12661 cttttattaa tgctgggcgt aatggttaaa agatttccac tagttcctct ccaccaagct
    12721 gagttgaaaa atgcttatct gtaaacaaca tagacaaaga ggaaataaac gtttccgtat
    12781 ctttctctgt cccaaggata ttaattaatt ttcctactag gtaagtatca ttaaacttaa
    12841 gatctgcctt cacatcaaaa gttttgataa aagcttttat catgcgtaca attttgtctt
    12901 tatcgccata attgatttca ccactcatat caatatagtc actaatccct gaagaaataa
    12961 tggcaactgc ttcttctttc gccttatcag ataattcatt taatgactgg gattgattga
    13021 tgacttcttg agttttatct gttacgtaat ctctcattat cctatcggca tactccgcaa
    13081 agtaatcttg aaaaaattct tctacagggt aaccaacttc gttttcccaa ccaggagaaa
    13141 tttcattatc agtacttatt aaatctatta aaaaccaacc cgttctctga gtgttgattc
    13201 tata
//
LOCUS       DAZKCN010000070        13146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000070 DAZKCN010000000
VERSION     DAZKCN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.071"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..149)
                     /locus_tag="ACG8K3_001916"
     tRNA            complement(77..149)
                     /locus_tag="ACG8K3_001916"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="ACG8K3_001917"
     CDS             530..1099
                     /locus_tag="ACG8K3_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP9145773.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="ACG8K3_001918"
     CDS             1086..1946
                     /locus_tag="ACG8K3_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9145774.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /gene="yycF"
                     /locus_tag="ACG8K3_001919"
     CDS             2136..2840
                     /gene="yycF"
                     /locus_tag="ACG8K3_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP9145775.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="ACG8K3_001920"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="ACG8K3_001920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP9145776.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /gene="yycH"
                     /locus_tag="ACG8K3_001921"
     CDS             4677..5993
                     /gene="yycH"
                     /locus_tag="ACG8K3_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP9145777.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="ACG8K3_001922"
     CDS             5994..6863
                     /locus_tag="ACG8K3_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP9145778.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="ACG8K3_001923"
     CDS             6918..7727
                     /locus_tag="ACG8K3_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9145779.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="ACG8K3_001924"
     CDS             complement(7830..8459)
                     /locus_tag="ACG8K3_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9145780.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="ACG8K3_001925"
     CDS             8629..9921
                     /locus_tag="ACG8K3_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9145781.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="ACG8K3_001926"
     CDS             complement(9997..10659)
                     /locus_tag="ACG8K3_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9145782.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="ACG8K3_001927"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="ACG8K3_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9145783.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="ACG8K3_001928"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="ACG8K3_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9145784.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DAZKCN010000071        13023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000071 DAZKCN010000000
VERSION     DAZKCN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.072"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            453..1025
                     /locus_tag="ACG8K3_001929"
     CDS             453..1025
                     /locus_tag="ACG8K3_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP9145785.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1170..1874
                     /locus_tag="ACG8K3_001930"
     CDS             1170..1874
                     /locus_tag="ACG8K3_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9145786.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="ACG8K3_001931"
     CDS             2102..2440
                     /locus_tag="ACG8K3_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145787.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /gene="lgt"
                     /locus_tag="ACG8K3_001932"
     CDS             2425..3279
                     /gene="lgt"
                     /locus_tag="ACG8K3_001932"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9145788.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="ACG8K3_001933"
     CDS             3426..3659
                     /locus_tag="ACG8K3_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP9145789.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3857..5761
                     /locus_tag="ACG8K3_001934"
     CDS             3857..5761
                     /locus_tag="ACG8K3_001934"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9145790.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            6071..6433
                     /locus_tag="ACG8K3_001935"
     CDS             6071..6433
                     /locus_tag="ACG8K3_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFP9145791.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6450..7061
                     /locus_tag="ACG8K3_001936"
     CDS             6450..7061
                     /locus_tag="ACG8K3_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFP9145792.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(7163..8071)
                     /locus_tag="ACG8K3_001937"
     CDS             complement(7163..8071)
                     /locus_tag="ACG8K3_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP9145793.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(8113..8427)
                     /locus_tag="ACG8K3_001938"
     CDS             complement(8113..8427)
                     /locus_tag="ACG8K3_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9145794.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8726..10657
                     /locus_tag="ACG8K3_001939"
     CDS             8726..10657
                     /locus_tag="ACG8K3_001939"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9145795.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10950..11501
                     /locus_tag="ACG8K3_001940"
     CDS             10950..11501
                     /locus_tag="ACG8K3_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9145796.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11814..12356
                     /locus_tag="ACG8K3_001941"
     CDS             11814..12356
                     /locus_tag="ACG8K3_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFP9145797.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            12867..>13023
                     /locus_tag="ACG8K3_001942"
     CDS             12867..>13023
                     /locus_tag="ACG8K3_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9145798.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSG"
BASE COUNT         4245 a         2169 c         2701 g         3908 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtgaaa tcagttaacg gtattgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa accaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg ttcttaaagc aaaagtcaag gccgtttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacgttct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 agtggatgat atagatgaag gggatatctt gctcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggtgaagc
     4381 tattccggtc aacaagctag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggaa aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcggaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagtaatgt ggatacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg ggaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa ggaactgaag ttgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc tcagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggctacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcagttg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcga aaagaacaaa aagaagtatc agcacctgca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaatgatt aaaaaattta ataataaaat
     5941 ttaaattcct attaagttgt aacacatgaa attgcttata atagtgtatt gaaattaagg
     6001 tagattgaca aacgagtgat caaatagata tactaccttc aaacgaccgt ttgaatgtga
     6061 ggtgagatga atggaaaaag tttgccaagt gactatcgta cataaagaaa aagtaaatgt
     6121 aataaaaaaa aaattggatc aaaaagattt ttcaaactta cttgtgttag ggaaatgttt
     6181 cagcgattct tctaggatta aaattttcta tgctttagaa acctataagg aaatgtgtgt
     6241 ctgtgattta gcagaaatac ttactgctag tgtcgctaca acgtcacacc atttacgttt
     6301 tttaaaaaaa catggaatgg caaaatcacg tcaagatgga aaagtagttt attattcttt
     6361 agcgaatgaa gacatccttt ccgctattcg tgttttttta aagttatcag aaaatctatc
     6421 tatgaaatct tagtttggag gaagttattt tgttacaaag tattttttca gctatcgctg
     6481 tttatatttc taccagtatt gattatttgt ttatcttgct gattatcttt tctcaaagcc
     6541 acacaaaaaa aggtattcgt cacatttttt ggggtcaata tcttggaaca ggtattttgg
     6601 tagccgtaag tttgtttgct gcttatgtgc taaattttat tccgcaagat tggatcattg
     6661 gtcttctcgg tctaattccg attttcttag gaatcagagt cgcttttgtt ggcgaagagg
     6721 aggaagaaga agaggaagtc gttgaaaaac tcgaatctag aggaactaat cgcttattct
     6781 ggacagttgc cttaataacg attgcttctg gaggcgataa tttaggtatt tatattccat
     6841 actttacctc gttagctttt tcagaaatca ttattgcttt aatcgtattc gctatctctg
     6901 ttgcggttct ctgctatatc agttacaaat tagccaagat ttcttttgtt tccgaaacgt
     6961 tagaaaaata cgaacgaatc attgtcccta taatgtttat tgcgttagga atttttatta
     7021 tgatagagaa cgggacgata caaacgctac taggttttta aaaatcataa tcacgaaaga
     7081 taaaaagaga ggcagcgact aatcaaaatc gctgcctttt ttgcttttca aataatgaag
     7141 tattttttag cgtagaacta tcttacgtgt tgtgggagta tcccttttca agctcttctt
     7201 tgcttgtagt tatttctatt gtgatatgat tctctacgct tgatttgtgt gcgacttctg
     7261 caatttcttc ttttatttcc ctagccttgt ctatgtctga agggtctata agaaccgtga
     7321 tgataagggc gttttcttcc ccgtccagtg accaaatgtg aaaatgtgag aggtctttta
     7381 cagcaggaac atttttaatg gagtccgcca agctctttac gtttacattt tcaggcgaac
     7441 cattcaataa aattttcatt gtggtgaaaa attcaggaac ggttttgtag aggatataaa
     7501 gtgcaatcaa aatcgataag ataggatcca gacgatatgc ctcggtaaag ttcattagaa
     7561 tactcacaat caagactcca atccacccta atacgtcttc cagcatatgg aggttcaaca
     7621 tgctctcgtt tttggatgtg cctttgctta aaatccacgc agcatatcca ttcaatgcaa
     7681 tagctacgag agaaagccag aacatgccac tataatttac gggttgtggg tcaatcaaac
     7741 gtggcacact acgatagatc ataaaaaacg agccactaat tagcacaacg ccagttatca
     7801 gcgcgcccag taaagaaaag cggttatagc caaaagtgaa ccgttcatct tgttttttct
     7861 ctgaataacc ttgaaaaaac caagcgaatc caattgaaat agagtcccct aaatcatgaa
     7921 ctgcgtcgga cataatggac acactattga aaagaaagcc aaagaaaaat tcgcttatag
     7981 agaaaataag atttatgaag aaagcaattt taatattctt tttcggttga ttctgattcg
     8041 agtgattatg gtctttatct gattttgaca tttaaaaacc tcctaagttt gtatgctaca
     8101 tactaattgg ttttattttt ctaattcatt aacatgagtt aagacttgtt cgagaatact
     8161 aaaaacatga aggtcatcta aactgtaaag catacttttt ccttctcttc ttgatttcac
     8221 taagcgtgcg tctttcaacg tttttaattg atgagaaata gcggactgtt ccatgtccag
     8281 agaaatagcg atatttccaa cactacactc ttttttctgt aaaagaaaga gaaccgacaa
     8341 tcgtgtagga tcactaatca ccttaaatag tttgcttatt gacagaatag atcctttatc
     8401 gattggagaa gttgttttta tatccatagt gtttttattc ctttcatatg accttccgtt
     8461 catatctata ttaccatatg agcgaaacaa tgctaatctt ttgtttataa aagagaagtg
     8521 gcgttctctt tatcttgaca aaatgtatat agcgatataa aatgagtata caaacatatg
     8581 attaattagt catatgtaag gtggattcat tacgatttac ttttggaata aacgaaagga
     8641 gtcagaaata gcagaaattt atgtgattca agaagtaaat gatggaaaac aaaggaatta
     8701 taaattatat agggaggcaa tagaagtgga aactatccaa gaacaacagt ctcaagaaaa
     8761 gcataatcat aatcacaacc acgatcacga ccatgatcat gggaaaatgc cagtcgtttt
     8821 gtactttgtt ggtttagcat tagcagtgat tgctctattt ttgaatgaag attatcagtt
     8881 ggtacaaaat gttttgttct caatcgctac aatcagtgcc gggtatcacg ttattcttct
     8941 tgaaggcctt ggagaaacaa ttgagaattc aaaagcacaa aaaaagttca tacctaattc
     9001 tcatatttta atgggattag cggcaattgg agcttctttg atggggaatt tttgggaagg
     9061 aacattattg attcttattt tttctggcgc acattttttg gaagattatg cagaaggaag
     9121 aagcaaacga gaaataacga aattactcga aatgaatcca acgactgccc ggttaatcct
     9181 gcctgatggt agtacaaaaa atgtggatgt cagtgaatta aaagtgggag atcaacttca
     9241 agtgttgaat ggcgatcaag tacctattga tggcgtcatt ttgtctggat ctacatccat
     9301 tgatgagtcg tctattactg gagaaagtat cccgaaagag aaatccaaag gagatggcgt
     9361 cttcgggagt acaatcaatg gaacaggtac cttcacgatg aaagtcacca aagaaaacaa
     9421 agatacagta ttttcaaaaa ttttacaatt agtgaatcag aaccaagata accaaacaaa
     9481 agctgcgagt attatccaaa aatttgaacc gaaatatgtc acagttgttt taattgccat
     9541 tcctctattc atgttattgg ctccttttct acttgattgg acatggtcac aaagtgtcta
     9601 tagaggattg gtccttttag ttgcagcttc accgtgtgct ttagcagcag ctactgtctc
     9661 ggcaacgcta tcgacgactt ctaatttagc caaaaaagga gtcctttcaa aaggtagttc
     9721 ttacctgtct caattagccg atattaaagc aattgcattt gataagacgg gcaccttgac
     9781 caaaggaaaa cctgaagtaa ccaattatta ttttgctgat tccgtgaacg aagaaaacat
     9841 gattgatatc gtagtagctc ttgaaaaaga atccaatcac cccttagcag atgctattct
     9901 aagaaagttt gagcaaaaaa acaaactaac tattgaagta gaaaaccaaa ttggtaaagg
     9961 attgacggga gattacaaag ggagaaatta tcgtatagga aaaccgactt cgtttgatga
    10021 tgtttcagat gaatatagtc gtttaaataa tgaatgggcg tcagaaggta agacagtcgt
    10081 atacgtagca gtagatgaag aggttatagg ccttattgcc cttatggacg ttccgagtga
    10141 atatgctaaa gaaaccattg aatattttag gaaacaaggt atacacacca cattaattac
    10201 tggtgactca gaaatgacgg gaaaagcggt tgctaaacaa ttaggaatag atgaagtgat
    10261 tgcgaatgtc atgccggaag acaaatccag aattatagac gaacaaaaag aaaaatacgg
    10321 cgtgaccgcc atggttggag acggtgtaaa tgatgctcct gctcttgtta atgcagatgt
    10381 gggaatcgct atgggagatg ggactgatgt ggcagtagag gtatctgatt tagttttaat
    10441 gcaaaacaat ttatctaaat tggtacaatc tcataatatt tcttcgaaaa tgaaccgtgt
    10501 tatttggcag aatattattt tttcaatggc cgttgttgct tttttaattg tcgttagttt
    10561 cttaggctta acggatattg caatcagtgt cattattcat gaaggaagta ctttggttgt
    10621 tattttgaat ggacttcgat tgctaaaggc agtctaaaga atgattgcac aacttcaaat
    10681 taatcgagta atagagataa ttaaggatta cactacattt tttatctcta ttacttgttt
    10741 atatttatct agatgttcaa agtcaaaatg atatatcaac catactcttg tggcgcgggt
    10801 tgaatataag atacatgaaa ttggataaaa tcatgtatca agaatcctat ccttataggg
    10861 ttcttttttt ttactccccc taaacacacg atttcgtgtt gtaataacta taatcatgta
    10921 ttcgaacaga ctaaaaagga gggattgtca tgggctataa tgttcagcca ctacggtcgc
    10981 aacaggagat taatgatttt ttattttgcc tgcgacggaa taaaaatgcg gaacgtgacg
    11041 ttttcttgtt tctaattgga attaacagcg gtctgcgcat gtccgacatc gttaaactca
    11101 agaaaaaaga cgtgattacc tcaaaaaacc cgcgcatcgt ggagcaaaag acagggaaaa
    11161 cacgcatttt atacttaagc agcttacagg acttgattca cggctatacc gcacccttag
    11221 atccggaggc ttacttgttt cccagtacta agggcggcca tttagaagtc aatacggtct
    11281 accagatgtt tcaaaaggtc gcgaagctgt taggaaggga cgatatcggc acccacaccc
    11341 tacgaaaaac ctttggttac cactattaca agaaaaccaa agacgtggcc accttaatgg
    11401 aaatctttgg tcacagcagc gagaagatta ccaaacgcta catcggaatt aatgaagatg
    11461 aaatcagtga aacgttatta aattttcggt taggtttttg attgtattat ataatagata
    11521 agcacagtaa gacggttata atcacccaca gtattggaca aatcaacctt tttttggtat
    11581 attatatata caaatgagag caggctcata gtcaacttga tataaatccg gaacataagg
    11641 gtccggcggg tggcgagcac caaaactaaa ctcgatacaa atccggaacg taagggtccg
    11701 gcgggtggca agcaccaaaa ctaaacctgt acaaaaggct gctcttttga gcagcttttt
    11761 ttattgggaa aaaggagcat tcttcgcacg cggcaaaaaa agaatacaaa cgcatgtcta
    11821 taaaagagac aacccaaatt accagacagt taaacgcgat ttataaggct gctcatctct
    11881 tacaagaaca ttttgtagat aaaaaagtat cctttgtcgg agaagtctca acggtagcga
    11941 ttattttttc gacaactaat tttatgcacc tctgtggcat tgattatcgc agaggcacac
    12001 acttgttttt tcaagatgcc ttagacagga aaataaattt acaggatatt caaatcaaaa
    12061 cggatgggac gacctttcag aaattacaag taataggtag ccttgattta ttattgggga
    12121 agcatatttc tattgttggc agaggtgttt actcttctct tcgctatgat gcagctattc
    12181 gaacaaggaa aaaaatattg gctctgtctt tgaaacaaaa cggattaatc tatatcccta
    12241 tctctttgtt aaacctatca tcaaaggaaa ttggacctgg acaaaaagtt actggcattt
    12301 ttagcgagga tttaacatcc ggggaactta aaatgattat ggaagtcatc gattaatcat
    12361 taatagtgat atgcgacgtc attttgaagt aattattcta ttttgggtta taaaataaag
    12421 cattttaaga cgatttcaaa tcattcatgt gggttattta tcacggatga gcttgaaatc
    12481 gtcttttata cgtatcaaat tgaaatgttc ggcatacaac ctatcgttgt tactacatta
    12541 ctaattgtca cttgttaagg aacttggatc ctttctaaac attccctaga gataaatttt
    12601 actttatccc tccgttaata tccaatttaa gcacctttcc gattcagggt taatttgcat
    12661 atcaagtgca gcttgtgttc tagtaagatt agccctttta gtccggctga gttgacttat
    12721 ggatataaga tatggtcata gcaaatagat actacgtata gtctgttaat ttagtgtcat
    12781 taatcttaag ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag
    12841 aagaaaacat accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag
    12901 aattcaagca acaaatcgtc aatctttact tagctggaaa gccacgtgta gaaatcattc
    12961 gagaatatga actaacggct tcagcatttg acaaatgggt aaagcaatct aaaacgagtg
    13021 gtt
//
LOCUS       DAZKCN010000072        12708 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000072 DAZKCN010000000
VERSION     DAZKCN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.074"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..676
                     /locus_tag="ACG8K3_001943"
                     /pseudo
     CDS             61..676
                     /locus_tag="ACG8K3_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(816..1856)
                     /locus_tag="ACG8K3_001944"
     CDS             complement(816..1856)
                     /locus_tag="ACG8K3_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9145799.1"
                     /translation="MASIVKRGKSYRAQVSLYKNGQHKKLSKTFATKKEATLWALEME
                     LAKGEGKELAERTTTFADFFENWIYLVKVNDIKETTFQNYVRTLGVIRNLFEDIQLKD
                     LNDIVVQKKIDEYAETHSRKTTHEVLLKIKTALRDAYARGYIANDFARLVKTRGVNPP
                     KRNKALSITNFKKLRNYVLQHPEDEFNVLVLLALETGMRRGELLAIKPENLYEYGIEV
                     RHSISPTSDDTSLKTQNAKRDVSINKEVYELIRQIPVKENGYIFSFGGFKQSEQLAEL
                     LESLGIEKTTFHGLRDTHASFLFAQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLSSL"
     gene            complement(1951..2148)
                     /locus_tag="ACG8K3_001945"
     CDS             complement(1951..2148)
                     /locus_tag="ACG8K3_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145800.1"
                     /translation="MSINKNTKDYPYLLTREQASRFLGIDPKSFDKYIRPHNDLERFM
                     VGKHERYTMKSLIDFIESQSV"
     gene            complement(2192..2395)
                     /locus_tag="ACG8K3_001946"
     CDS             complement(2192..2395)
                     /locus_tag="ACG8K3_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145801.1"
                     /translation="MSNKKAMENINHIIEMIDFFQSGNGVINKKDNDSDYLRHIYTPE
                     DILGNLQHSAKEALKQLKNGELK"
     gene            complement(2405..2893)
                     /locus_tag="ACG8K3_001947"
     CDS             complement(2405..2893)
                     /locus_tag="ACG8K3_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145802.1"
                     /translation="MKHMRHIAKEVDDWTRVEAEFSGDYAHQLTDAIKECSTDEQLKN
                     LIISSLLDRYMFFYVNSNRPHKITRLMLELLDEKNFHFESPSPRNNLLEQSIDHLIKG
                     SGLLPTLWKVQQIWGGDTAKELIDYLYKQYYEEFEPNDDHIAWVNKYKNIYLLDGKPW
                     KE"
     gene            complement(2890..3084)
                     /locus_tag="ACG8K3_001948"
     CDS             complement(2890..3084)
                     /locus_tag="ACG8K3_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145803.1"
                     /translation="MASTNKSYLQKVIDDNKEKPYKRNNSKVTDVSKDFQAQTLYIGA
                     RSRNTKVHLRIYDKKEEQLS"
     gene            complement(3071..3562)
                     /locus_tag="ACG8K3_001949"
     CDS             complement(3071..3562)
                     /locus_tag="ACG8K3_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145804.1"
                     /translation="MIKPDIDQFTLVLQTTGVFDFDEWRDWVAKSLISTFLIKSKMHK
                     LFDNFGEADVKLPEGYTIGYSFINAPFYFCIAYHEAFSKMGVIVKYSAYAWHEYRKRY
                     EAEFNEPIHLHTFLKMIDSDEYSFRLSRIDICYDFINEGIDIAKLKRSIEEKRTELRY
                     GKY"
     gene            complement(3791..5092)
                     /locus_tag="ACG8K3_001950"
     CDS             complement(3791..5092)
                     /locus_tag="ACG8K3_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP9145805.1"
                     /translation="MKKTYRMQRGFISFLFIGFFLILWFFFSKLYPLIVEQITIIQLP
                     HIESNIFLFPVGLVVVGLFVSWSIRNKLWFGLFFSVHYYLMIKRIRRHVKDARFEDER
                     EFDNRLVKLPIIKIEFDDNRARKSGKVFIQNSIKFDKKLEDMRIDAALEGYVSERQYL
                     SKDRNWYIYEFYDVDSQKQLEIKSASDLIEWSNKKTDDYSLRLDERATVPFHHMGLVG
                     QTGSGKSFFIQMLVEQVLSKKVSHELFVIDPKRTDVYQMAKRTVGDERTADKTNAIEL
                     INQFHERMKKRQDELQDYFISNRNKTYRDAGLPALILLIDEFGALRESWKTLAKKERD
                     EVDSILSDVAFMGRQLGCILWVATQQMNAQTMPTAIREQLVLKIALGDSDEQTYRTLF
                     SSGVDIPPVQFTAGQGIYSYPGLASVDKPRMLAVPYCSYLE"
     gene            complement(5204..5551)
                     /locus_tag="ACG8K3_001951"
     CDS             complement(5204..5551)
                     /locus_tag="ACG8K3_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145806.1"
                     /translation="MKKLRQFNKFDAEVFFKDKDVRVMAHEPWNEYEDGQVTKELGTK
                     YKCVIATDNTKYEGNDVQPDLNAGEQIDVKVTQPPKSYKKFSRITFVNPTASVYGTFM
                     NELSVKADDVDIV"
     gene            5727..6314
                     /locus_tag="ACG8K3_001952"
     CDS             5727..6314
                     /locus_tag="ACG8K3_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP9145807.1"
                     /translation="MLNDKADDMLNEEVAQNEILDFIDMKYGANQLVTQRTIQRWLEE
                     LGIDCIQPRPKRNRDIRYRKSDVLKLEEIKRTNLLRKKNKELARKDIEKIATDMQKKE
                     IEYNQWLASMTDVDRRQQDIDDMYDYKVPALNKIREDKLEMCFRKLFPDVKFDIEYLE
                     KILFTINEPSMHSDLEYGQAIVDLKEKAYIKNYKD"
     gene            6728..7171
                     /locus_tag="ACG8K3_001953"
     CDS             6728..7171
                     /locus_tag="ACG8K3_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003726066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP9145808.1"
                     /translation="METILEIVRIEQVICEAEEGVNYTIKGPKDGAEIASRFIGRDDR
                     EVFFVMCLNTKNHVVAVHRCHVGSLNASIVHPREVFKSAILNNAASIIVAHQHPSGDI
                     TPSMEDINVTKRLVEAGKLLGIEVFDHLVVNGDSKFTSLKEKGHF"
     gene            7299..8045
                     /locus_tag="ACG8K3_001954"
     CDS             7299..8045
                     /locus_tag="ACG8K3_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010721123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9145809.1"
                     /translation="MTEEIIKCKTRVQKHGEVFTPKRIVNQMLDIPEIKEACENLTTT
                     FLEPSAGEGAFLVEILERKLKMVSEKYNKDLVQFENYSLLALTTLYGIELLEDNAQTC
                     VMNMFQKYYDYYKEQIQYHKGKMNQKVLDSAKRIISLNIGNGNFLTRKAVDGTPLIFS
                     EWEAINLRKNTKNIKVQRTEYTFDEIYENTQKENGKALRQPKTEPEQIDIFSFLDEPI
                     DKKEEVVKEMQYIPVKIIDVYKEEMEEANG"
     gene            8038..12378
                     /locus_tag="ACG8K3_001955"
     CDS             8038..12378
                     /locus_tag="ACG8K3_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFP9145810.1"
                     /translation="MDKTVIKPFQEIKYKVYGYTLPEVPNHDGYVKIGDTTRDAVTRI
                     FEQVGTAGLNPKILFEKLARKSDGKWFRDKDLHKFLILNGIEKKNFNSRADEWFYFNG
                     TLEKAEELTNKYINRDYDEIQIDDKRSDYVLRNEQQQAVEKTYEYYQSKQEPKEFLWN
                     AKPRFGKTLTTFDFIRNLNATNVLIVTNRPAIANSWFDDFHKFIAWQEPQMKFVSETD
                     ALNGKAMTRDQYLEFIYTTSHVNPTQIAFISLQDLKGAEFAGGMYKKLEWVAGLNWDL
                     LVIDEAHEGIDTRKTDIAFSQLKREFTLHLSGTPFKALAKGDFSEEQIFNWSYVDEQE
                     AKENWDPTIGSNPYESLPTLNLFTYQMSKMIEDEVSAGLTIEEEKNVDYAFDLNEFFR
                     VKENGRFEYEESVVRFLDNLSSGKFPFSENQYRNELNHTFWLLPRVNAAKALEKLLNA
                     HPTFKDYKVVLAAGDGVSLHDGAEAEALDLKENTKSFDKVRNAIEQNDKTITLSVGQL
                     TTGVTIPEWTAVFMLNNIESPSLYFQAAFRSQNPYEYEKDGKLFRKENAYIFDFSPDR
                     TLRLYDEFANNLSSGSSKTSEERKRKIKKLLNFFPVIAEDEEGTMHEIDASEVLTIPT
                     RITSREVVKRGFMSNLLFANISGIFSGDSPFKEILDKIPPEKNKRLEKPREVSITDPH
                     LNDEGEVEIPKEVVLGKSKELFGEKIYSSIEVVDEVVSSFEEAEEKQKESIKKLAKDI
                     TASFSDGFNNLKESFKLNKGQTEKLKESVNNSIEDIITEKSLTLSNQVKEIEEEYTKK
                     KQEAEKKNDETELKAAIQQYEEEKQKIEESFADDINKEVENTINTAVEEQIEKVEEKK
                     KKTTEDDVRDHLRGFARTIPAFLMAYGDNQTRLENFEENIDEATFEDITSITIEEFKK
                     LRDGFEYEDDNGNRKVIHGLFNEVVFNASIREFLDTKKRLANYFDESLEEDIFDYIPP
                     QKTNQIFTPKRVVKKMVDKLEEENPGIFSDRTKRFADLYAKSGLYITEIVKKLNEGLK
                     GQIPNQQERIKWILENQVYACAPSNIICNIVKNFVYSHTINVSTKNLIEVDTMKLASE
                     EKLSTEIYELFGDDRLKFDVIIGNPPYQDETIGENKTYAPPIYHKFLEESYKLADKVE
                     MIHPARFLFDAGSTPKAWNRKMLADENLKVIDYVDDSSKVFPNTDIKGGIAITYRDAQ
                     KRIGPIGTFTKYPELNTILRKVKMDNFNSIDKIIYSADSYKFTQALHDDYPDAEARLS
                     KGHAFDVRSNVFDSIGHIFKNEKPSDNSEYIRIIGRQNHKRIEKWVKRDYIGEHPNLD
                     KYSVLLPGSNGNGDFGETFASPIIKEAAVAHTQTFISIGHFDNIIEAQNLLKYVKTKF
                     ARAMLGILKATQANSRPKWKWVPMQNFTNESDIDWSESISEIDKQLYKKYNLNQNEIQ
                     FIEDKVKPME"
BASE COUNT         4448 a         1996 c         2326 g         3938 t
ORIGIN      
        1 agagttgggc aacagaattt aaaaatgatc cggatttcag aattttaaag gagttttatt
       61 atggcgaaaa ttgggtatgc tcgagtaagc agcttagatc aaaatttaga ccgacaatta
      121 gaattattat cagagtgtga taaaattttt agtgataaag caagtgggaa agataccaat
      181 cgagaaggat ttcaagaaat gatgaaattc atacgtgaag gtgatattgt cgttgtgaca
      241 gaattaaaac gacttggacg aaataataaa gagctaacag aaacgatgaa tttaattcag
      301 ctgaagaaag ctacactgga agttttgaat ttgccagcgt taacaggtat cactgatgat
      361 aatttacgtc gattacttaa taacttaatt atcgaactat acaagtacca ggccgaagaa
      421 gaacgtcggt atattcgcga agcaacaaca aggaattgca ctcgctaaag caaaagggaa
      481 atacaaggga ggtacgctaa aatatcgtga gaatgatccg cgacttcaat tagcatttaa
      541 tttgtttttg actggatcta cagataaaga agttgaagag ctaacaggga ttaatcgtag
      601 aacgtttaga agatatcgaa tgaaacatgg tattactaat gaagttaggg agcaaaaaaa
      661 taaatctttc agttgaggct aatataattt tgcaagaaac caggtcttaa gaaagctcgt
      721 aaagcttcac aattctcaaa acgttaatag gtttgcctat ataccaacgt ttacaaaggt
      781 tttttggttc aagttggttc aaatcagctt gaacctcata aagaacttaa cagatttaaa
      841 gcatcggcat cttgctggtg ctttttttct ggcattaatt ctaggtagta attttgggtt
      901 gtttggatgt taatatgccc taaccttttc gatacataag caatatcaat atcttgtgca
      961 aacaagaatg atgcatgtgt gtcacgtaaa ccatggaagg ttgttttttc aatacctaaa
     1021 ctctccaata gctccgctaa ctgttcggac tgtttaaatc ctccaaaact aaaaatatac
     1081 ccattttctt ttaccggaat ttgacgaatt aactcatata cttctttgtt aattgacaca
     1141 tcacgctttg cgttttgtgt ttttaaagac gtatcatcag aagtaggact gattgaatgc
     1201 ctaacttcga taccgtactc gtaaaggttc tctggtttaa ttgcaagtag ttctccacgt
     1261 cgcatacctg tttcaagtgc aagtaataca agtacattaa attcatcttc tggatgctgt
     1321 aagacgtaat tacgaagctt cttgaagttt gtaatggata atgccttgtt gcgttttggc
     1381 ggattgacac cacgagtttt tacaagtcgt gcaaagtcat ttgcaatata tccccgagca
     1441 tatgcatcac gtaaagcggt tttaattttt aacaatactt catgtgttgt tttacgagaa
     1501 tgagtttccg catattcgtc aattttcttt tgaacaacaa tatcgtttaa atcttttaac
     1561 tgaatatctt cgaacaagtt gcgaatgact cccaatgttc gaacgtaatt ctgaaaagtc
     1621 gtttctttta tatcattcac ttttacaaga tagatccagt tttcaaagaa gtcagcaaat
     1681 gtcgttgttc gttctgcaag ttctttcccc tcaccttttg ctaattccat ttctaaagcc
     1741 cataaagtag cttctttttt ggttgcaaat gttttagaaa gctttttatg ctgtccgttc
     1801 ttatagagtg acacttgggc tctatatgac ttcccgcgtt ttacaatact agccatacta
     1861 tcaattcctt tcaaaaaata agttgataga tgctagttga atacacctgt attgtattct
     1921 gtaactagca taatatcaac tgttaaaaca ttagacagat tgtgattcga taaagtcaat
     1981 taaagatttc attgtatatc gttcgtgttt tcctaccata aaacgttcaa gatcattatg
     2041 tggacggata tatttgtcga atgactttgg gtctatgcca agaaaacgag atgcttgttc
     2101 acgagttaaa aggtaaggat agtctttagt gtttttattg atgctcatgt agcaccatct
     2161 cctttgtaat aaaataatat ccaagtaata atcacttcag ttctccattt tttaactgtt
     2221 ttaatgcctc tttagcagag tgttggagat tacctagaat gtcttcaggt gtatagatgt
     2281 gacgcaagta gtcagaatca ttatcttttt tattaatcac accattacca ctttgaaaaa
     2341 aatctatcat ttcaataatg tgattaatat tttccattgc ttttttattg ctcatatcgt
     2401 aatttcactc cttccaaggt ttaccatcaa gtaaatatat atttttatat ttgttcaccc
     2461 atgctatgtg atcatcattg ggttcgaatt cttcatagta ctgtttatat aaataatcaa
     2521 ttaattcttt tgctgtgtcg cctccccata tttgttgaac tttccacaat gtggggagta
     2581 gaccagaccc cttaattaaa tggtctatcg actgctctaa taagttattt cgtggagatg
     2641 gtgattcaaa gtgaaagttc ttttcatcta acaattcaag cattaatcta gttatcttat
     2701 gaggtcgatt agaattaaca taaaagaaca tgtagcgatc aagtagagaa ctaataatca
     2761 aatttttaag ctgttcatct gtactacatt ctttaattgc atctgttaat tgatgggcgt
     2821 aatcacctga aaactctgct tcaacacgtg tccagtcatc cacttcttta gcgatatggc
     2881 gcatgtgttt catgacagtt gttcctcttt tttatcataa atgcgtaaat gcacttttgt
     2941 atttctgctt cttgccccga tatacaatgt ttgtgcttga aagtctttgg atacgtcggt
     3001 cactttcgag ttgtttcttt tgtatggttt ttctttgttg tcatcaataa ctttttgtag
     3061 ataactttta ttagtacttg ccatatcgca attcagtcct tttttcttca attgagcgtt
     3121 ttaatttagc aatatcaata ccttcattaa taaagtcata acagatatca attcgtgaaa
     3181 gtctaaaact atattcatcc gaatcaatca tttttaaaaa ggtgtgtaaa tgaataggtt
     3241 cattaaattc tgcttcatat cgctttcgat actcatgcca tgcatatgca ctatatttga
     3301 caatcacacc cattttcgaa aatgcttcat gataagcgat acagaaataa aaaggtgcat
     3361 tgatgaatga gtaacctatt gtgtagcctt ctggtagctt aacgtcagct tcaccgaagt
     3421 tgtcaaatag cttatgcatc ttagatttaa tcaagaaagt acttattagg ctttttgcta
     3481 cccagtcacg ccattcatca aaatcaaaca cgcctgttgt ttgtaatacg agtgtaaatt
     3541 ggtcaatatc tggcttaatc atgttctatt cgctcctttt cctaagaaaa ctttaggtaa
     3601 agtttacttg gtgtaaaagc ctataagaat aggctttagt ctgggtttta ctcctgactg
     3661 gagctggggt gattaccaat cccccagctc tcctaaagga gggaagcacg gagcggaacc
     3721 tttccctgcc taacggcggg tccctttccg ctcattgtgc aaccgttcct taagaaaaag
     3781 ctaagtgcta ttattcaaga tagctacagt atgggacagc aagcattctc ggtttatcaa
     3841 cacttgctag ccctggataa ctgtaaatcc cttgaccagc agtaaattga actggaggta
     3901 tatccacacc agaagaaaac agagttctat aggtttgctc atcactatca cctaaagcaa
     3961 tcttcaaaac gagctgttct ctaatcgctg ttggcatcgt ctgtgcgttc atttgttgcg
     4021 tagcgaccca cagaatacac cctagttgac gacccatgaa agcaacatca gatagtatgc
     4081 tatcgacttc atcacgttct tttttcgcta aagtcttcca tgattctctt aatgccccga
     4141 attcatctat aagcaaaatt aaagccggca aaccagcatc cctatacgtt ttgttacgat
     4201 ttgatatgaa atagtcttgt aattcatctt gtcgtttttt catacgctca tgaaattgat
     4261 taatcaattc aatcgcattt gttttatcgg ctgttcgctc atcaccaacg gtacgtttag
     4321 ccatttggta aacgtcagta cgctttggat caattacgaa taactcatga ctgacctttt
     4381 ttgataatac ttgttcaact agcatttgaa taaaaaacga cttaccacta cctgtttgcc
     4441 caactaatcc catatgatga aacggtacag ttgcacgctc atctaaacgt agggagtaat
     4501 cgtcagtttt cttgtttgac cactcaatca agtcacttgc tgatttaatc tcaagttgtt
     4561 tttgtgaatc tacatcatag aactcataga tgtaccaatt acggtcttta gacaaatatt
     4621 gacgttcact tacgtatcct tctaacgctg catcaattct catgtcttct aacttcttgt
     4681 cgaatttaat tgagttttga ataaacactt ttcctgattt ccttgcgcga ttatcatcaa
     4741 attcaatttt gattatcggt aatttaacta aacgattatc aaactcacgt tcatcctcga
     4801 atctagcatc tttaacatgt cttctaattc tttttatcat caaataataa tgcacagaaa
     4861 aaaatagacc gaaccataat ttattgcgaa ttgaccaact aacgaacaaa ccaacaacca
     4921 ccaagcctac aggaaacaag aagatatttg actcaatatg tgggagctgt atgatcgtta
     4981 tttgttcaac aatcaatggg tacagtttag aaaagaaaaa ccataaaata agaaaaaatc
     5041 caatgaataa aaatgaaata aaaccacgtt gcatccgata cgttttcttc acatattcca
     5101 cgtcctaata ttgtggttcc gcccctgata aggggcggtc acaggtataa aagtgtattt
     5161 gacgatagaa agggaactga ctaccgttcg ttctttactt cggttaaaca atgtctacat
     5221 catctgcttt gacagaaagt tcattcatga acgttccata cacagaagct gtcggattca
     5281 caaacgtgat tctactaaat ttcttataac tctttggagg ttgtgtcact ttcacatcaa
     5341 tttgctcacc agcatttaaa tctggctgaa catcattgcc ttcatatttt gtgttatccg
     5401 tcgcaataac gcacttgtac ttagtaccta actcttttgt gacttggccg tcctcatact
     5461 cgttccacgg ttcatgagcc atcactcgaa cgtccttatc cttgaaaaaa acctcggcat
     5521 cgaacttatt aaactgtctt aatttcttca tagtgttttg cactccttta tttttgatct
     5581 acctatattt tagctaatac ttgccatttt atcaagacat gtcatcatga caaataacaa
     5641 aaattgttgc acttatagta tgttaatggt attataattt ataatgacat tcgataattt
     5701 ataacgacat ttatgggggt gttaaaatgt taaatgataa agcagatgat atgttaaatg
     5761 aagaggttgc acaaaatgaa atattagatt ttatagatat gaaatatggg gcaaaccaac
     5821 tcgtgactca aaggacaata caaagatggt tagaagagtt aggcattgac tgcatacaac
     5881 ctcgtccaaa gagaaatagg gatataaggt ataggaaatc tgatgtctta aagctagaag
     5941 aaataaagag aactaattta ttgaggaaaa agaacaagga attagcacgt aaagatatag
     6001 aaaaaatagc aaccgatatg cagaaaaaag aaatagagta taaccaatgg ttagctagca
     6061 tgacagatgt tgatagaaga cagcaagata ttgatgacat gtatgattat aaagtacctg
     6121 cattaaataa aattagagaa gacaaattgg aaatgtgttt tagaaagttg tttcccgatg
     6181 ttaagtttga tatagagtac ttagaaaaga ttttgtttac cataaatgaa ccatccatgc
     6241 attcagattt agaatacgga caagcaatag ttgacttaaa ggaaaaggct tatataaaaa
     6301 attataaaga ttgaaagctc atagaagaaa gggagcaaac ccgttaatcg ttatcacaat
     6361 aaagtaggat aaaatttatt tgaatttgta atagggcaag tgcagatagg gagtaggtac
     6421 atccgtcaga tttttccttt tcagctcccc taattgtaat ctcctaatgc gtccgtaaag
     6481 aatggacgca ttatcgctta tgtaaagggt cttcgttctt cggatataat ggcaaagcca
     6541 agtggataag agtgcgtaat aaagcaatta acgctttaaa cgcactctaa tttattgggc
     6601 tttactcatc atatcaacga atctcttgat agctgtcgct acttacgcca cttgacatta
     6661 atcgatgcgg agattgattg aatggtgacc aaaaaggtca aaaataacac gagagaggtt
     6721 gatctacatg gaaaccattt tagagattgt acgtatcgaa caagttattt gtgaggctga
     6781 ggagggtgtt aattacacga taaaaggtcc aaaagatgga gcggaaattg catctcgttt
     6841 tattggacgt gatgatcgtg aagtattctt tgttatgtgt ttaaacacta agaatcacgt
     6901 tgtcgcagtt catcgttgtc atgttggttc attgaatgct agtatagtac atccgcgtga
     6961 ggtgtttaaa tcagcgatac ttaacaatgc agcaagtatc attgttgcgc atcagcatcc
     7021 aagtggtgac ataacaccat caatggaaga tattaatgtt acaaagagat tagttgaggc
     7081 aggaaagcta ttaggcattg aagtgtttga tcatttagtg gtaaacggtg atagtaaatt
     7141 tacaagttta aaagagaaag ggcattttta aaagggagga atctcccttt ttcttcaagt
     7201 aattacggaa ctaccgaaaa gttacgttct taagctgatt atggtaaaat ggaagcagat
     7261 aatatgcgaa agcttacata taaagggggg gggagaattt gacagaagaa attattaaat
     7321 gtaaaactcg tgtacaaaaa catggggaag tatttactcc taaaagaata gtaaatcaaa
     7381 tgctagatat accagaaata aaagaagcct gtgaaaatct tacgacaaca tttttagagc
     7441 catctgctgg tgaaggtgcg tttttagttg agatacttga gcggaaatta aaaatggtat
     7501 cagagaagta taataaagat ttagttcaat ttgaaaatta ctctttacta gccttaacta
     7561 cattgtatgg aattgaattg ttagaagaca atgcccaaac atgcgtgatg aatatgtttc
     7621 aaaagtatta tgactattat aaagagcaaa ttcaatatca taagggaaaa atgaatcaaa
     7681 aagtattaga tagcgctaaa agaattatct ccctaaatat tggcaatggt aactttttaa
     7741 ctcgtaaggc agtagatggt acgccattga tcttcagtga gtgggaagcc attaatttaa
     7801 gaaaaaatac taaaaatata aaagttcaaa gaacagaata tacatttgat gaaatatatg
     7861 aaaatacaca aaaggaaaat gggaaagccc taagacaacc aaaaactgaa ccagaacaaa
     7921 ttgatatttt tagcttttta gatgaaccaa ttgataaaaa agaagaagtt gtaaaagaaa
     7981 tgcaatacat tcctgtgaaa ataatcgatg tttataaaga ggagatggag gaagccaatg
     8041 gataagacag taattaagcc ttttcaggaa attaaatata aagtgtatgg ttatacctta
     8101 cctgaggttc caaaccacga tggttatgtg aagataggtg atacgacacg tgacgctgtt
     8161 acgagaattt ttgaacaagt aggtacagct ggtttaaatc ctaaaatatt gtttgaaaaa
     8221 ctagctcgta aatctgatgg aaaatggttt cgtgataaag atttacataa atttcttatt
     8281 ctaaatggaa tagagaaaaa gaattttaat agtcgtgctg atgagtggtt ctattttaat
     8341 ggaacgctgg aaaaggctga agagttaacg aataagtata taaaccgtga ttatgatgaa
     8401 atacaaattg atgataaacg ttcagattat gtattaagaa atgaacagca acaagcggta
     8461 gaaaaaacgt atgaatatta tcaaagtaag caggagccaa aagagttttt gtggaatgca
     8521 aaaccgcgtt ttgggaaaac attaacaaca tttgatttta ttagaaacct taatgcaacg
     8581 aatgttttaa tcgtaacaaa tagacctgcg attgctaact catggtttga tgatttccat
     8641 aaatttattg catggcaaga gccacaaatg aagttcgtat cggaaacgga tgcactaaat
     8701 ggaaaagcaa tgactagaga tcagtatctt gaatttattt atacaacttc ccatgttaat
     8761 cccactcaaa ttgcatttat ttcattgcag gacctaaagg gtgcagaatt tgcaggaggt
     8821 atgtataaga aactagaatg ggtagctgga ctaaactggg acttgctcgt aattgatgaa
     8881 gctcatgaag gtatcgatac tagaaaaact gatatagcat ttagccaatt aaaaagggaa
     8941 ttcaccctgc atttatcagg tactccgttt aaagccttag ctaaaggaga tttttctgaa
     9001 gaacaaatat ttaattggtc gtatgtagat gaacaagaag ctaaagaaaa ctgggatcca
     9061 acaattggta gcaatcctta cgaaagctta cctactttga atttatttac gtatcaaatg
     9121 agtaaaatga tagaagatga agtatcagca ggattaacaa tagaggaaga gaaaaacgtt
     9181 gattatgcat tcgatttaaa tgaatttttt agagtgaaag aaaacggaag atttgaatat
     9241 gaagagagtg ttgttcgctt tttagataat ctatcttcag ggaagtttcc attttctgaa
     9301 aatcaatatc gtaatgaact aaatcataca ttttggctgt tacctcgtgt gaatgcagca
     9361 aaagcacttg aaaaattgtt aaatgctcat cctactttta aagattataa agtcgtactt
     9421 gcagcaggag atggtgttag tctgcatgat ggagcagaag cagaagcgct tgatttaaaa
     9481 gaaaatacaa aaagctttga taaagttaga aatgcaattg agcaaaatga taagacgata
     9541 acactttctg tcgggcaact gacaactggt gtgacgattc cagaatggac agctgtattt
     9601 atgctaaata atattgaatc tcctagttta tactttcaag ctgcattcag gtcacaaaac
     9661 ccttatgaat atgaaaaaga tggaaaactt tttaggaaag aaaatgcgta tatctttgat
     9721 ttttcaccag atcgaacatt aaggctttat gatgaatttg ctaacaactt gtctagtggt
     9781 agttctaaaa caagtgaaga aagaaagcgc aaaataaaga agttattaaa tttctttcca
     9841 gtaattgctg aagacgaaga aggtacaatg cacgaaatag atgcatctga agtactaacg
     9901 atacctacac gaattacttc acgagaagtt gttaaaagag gctttatgag taatttacta
     9961 tttgctaata tatcaggtat cttttcagga gattcaccat ttaaagagat tttagataaa
    10021 ataccacctg agaaaaataa aagattagaa aaacctcgtg aggtttctat tactgatccg
    10081 catttaaatg atgaagggga agtagagata ccaaaagaag tagtgttagg taaatcaaaa
    10141 gaacttttcg gagaaaaaat ttattcatca attgaagtgg ttgatgaagt tgtatcatcc
    10201 ttcgaagaag ctgaagaaaa acaaaaagaa tctattaaaa aacttgcgaa agatataaca
    10261 gcatcatttt cagatggatt taataactta aaggaatcct tcaaactgaa caaaggacaa
    10321 accgaaaagc taaaagagtc agtaaacaat tcaattgagg atattataac tgagaaaagt
    10381 ttaacactaa gcaatcaagt taaagagata gaggaagaat atacaaagaa aaagcaagaa
    10441 gcagaaaaga aaaatgatga aacagaatta aaagcagcga ttcagcaata tgaagaagaa
    10501 aaacaaaaga tagaagagtc ttttgccgat gatattaata aagaagtaga aaacacaatc
    10561 aacactgctg tcgaagaaca aatagaaaaa gtagaagaaa agaaaaagaa aacaacagaa
    10621 gatgatgttc gagatcactt acgaggattt gcacgtacaa tacctgcatt cctaatggca
    10681 tacggagata atcagactag attagaaaac tttgaagaga atattgatga agctaccttt
    10741 gaagatatta caagtatcac aattgaagag ttcaagaagc ttcgcgatgg ttttgaatat
    10801 gaggacgata atggtaatag aaaagtcatt catggtttat tcaatgaggt agtttttaat
    10861 gccagtatac gagagttttt agatactaaa aagcgtttag cgaattactt tgatgaaagt
    10921 ttagaagagg atatttttga ctatattcct ccacagaaaa cgaatcagat ttttacgccc
    10981 aaaagagtag taaaaaaaat ggtcgataaa ttagaagaag aaaatcctgg gattttttct
    11041 gataggacga aaaggttcgc cgacttgtat gcgaaaagtg gtttatatat tacggagata
    11101 gtaaagaaat taaatgaagg tttgaaagga caaattccaa atcaacagga acgtattaaa
    11161 tggatacttg aaaatcaagt atatgcgtgt gcacctagta atattatttg taacattgtt
    11221 aaaaactttg tctatagtca tacgataaat gtgtcaacca aaaatcttat agaagttgat
    11281 acaatgaagt tagcaagtga agagaagtta agtactgaaa tctatgagtt atttggagat
    11341 gatagattga aatttgatgt aattattggt aatcctcctt accaagatga aacgatagga
    11401 gaaaataaaa cgtatgctcc accaatttac cacaagtttt tggaggagtc ttataaacta
    11461 gctgataaag ttgaaatgat tcatcctgct cgatttttat tcgatgctgg tagtacacca
    11521 aaagcatgga atagaaaaat gttagccgat gaaaatttga aggttattga ctatgtggat
    11581 gatagctcaa aagtatttcc taacaccgat attaaagggg gtatagcgat aacctaccgt
    11641 gatgctcaaa agagaatagg tccgatcggg acctttacaa agtatccaga attaaatact
    11701 atccttcgta aagtgaagat ggataatttt aattcaattg ataaaattat ttattcagca
    11761 gatagctata agtttacaca agcattacac gacgattatc ctgatgcaga ggcacgatta
    11821 agcaaaggtc atgcatttga tgttcgctct aatgtatttg atagcattgg acatatattt
    11881 aaaaatgaaa agccctcaga taatagtgaa tatattagga ttataggtag acaaaatcat
    11941 aaaagaatag aaaaatgggt gaaacgtgat tatattggtg aacacccaaa tttagataaa
    12001 tatagtgttc ttctaccagg atcaaatgga aatggtgact ttggagaaac atttgcttct
    12061 cctataataa aagaagctgc tgttgcacac acacaaacgt ttattagtat tggtcatttt
    12121 gacaatataa tagaagctca aaacctgtta aaatatgtaa aaacaaagtt tgcgcgagca
    12181 atgttaggaa tattaaaagc aactcaagct aatagtcgac caaaatggaa gtgggttcca
    12241 atgcaaaact ttactaatga atcagatatt gattggtcgg aatcaatatc tgaaattgat
    12301 aaacaactat ataaaaaata taatttgaac cagaatgaaa ttcagtttat tgaagataaa
    12361 gtaaaaccaa tggaataaaa tataagcaag acattgttgt attcaaccag catctatggt
    12421 tcaaattggt gcaagtaatg tcaatttcaa tcctttttca tacattttca tccatataat
    12481 aattcccaaa acggtgatat aaatggattg cgatatattc aatagactta gaattaatct
    12541 gtatatttct caaaacgtta atcgttgtta tatcaaggat ttcacggcat catccgtttg
    12601 gatggtgtct tttttgggtt gaattccttg aaaattgggt ctattttaca gacccttttc
    12661 tatataaact gttttcgata cttggaaaaa tctgttgttt ttaaattt
//
LOCUS       DAZKCN010000073        12487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000073 DAZKCN010000000
VERSION     DAZKCN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.075"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..732)
                     /gene="frr"
                     /locus_tag="ACG8K3_001956"
     CDS             complement(175..732)
                     /gene="frr"
                     /locus_tag="ACG8K3_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9145811.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(735..1457)
                     /gene="pyrH"
                     /locus_tag="ACG8K3_001957"
     CDS             complement(735..1457)
                     /gene="pyrH"
                     /locus_tag="ACG8K3_001957"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9145812.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1593..2474)
                     /gene="tsf"
                     /locus_tag="ACG8K3_001958"
     CDS             complement(1593..2474)
                     /gene="tsf"
                     /locus_tag="ACG8K3_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9145813.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2573..3355)
                     /gene="rpsB"
                     /locus_tag="ACG8K3_001959"
     CDS             complement(2573..3355)
                     /gene="rpsB"
                     /locus_tag="ACG8K3_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9145814.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3714..4193
                     /locus_tag="ACG8K3_001960"
     CDS             3714..4193
                     /locus_tag="ACG8K3_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9145815.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4410..5501
                     /gene="phnW"
                     /locus_tag="ACG8K3_001961"
     CDS             4410..5501
                     /gene="phnW"
                     /locus_tag="ACG8K3_001961"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP9145816.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5494..6279
                     /gene="phnX"
                     /locus_tag="ACG8K3_001962"
     CDS             5494..6279
                     /gene="phnX"
                     /locus_tag="ACG8K3_001962"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP9145817.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6625..8316)
                     /gene="argS"
                     /locus_tag="ACG8K3_001963"
     CDS             complement(6625..8316)
                     /gene="argS"
                     /locus_tag="ACG8K3_001963"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9145818.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8738..9685)
                     /gene="arcC"
                     /locus_tag="ACG8K3_001964"
     CDS             complement(8738..9685)
                     /gene="arcC"
                     /locus_tag="ACG8K3_001964"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9145819.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9800..10819)
                     /gene="argF"
                     /locus_tag="ACG8K3_001965"
     CDS             complement(9800..10819)
                     /gene="argF"
                     /locus_tag="ACG8K3_001965"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9145820.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10910..12139)
                     /gene="arcA"
                     /locus_tag="ACG8K3_001966"
     CDS             complement(10910..12139)
                     /gene="arcA"
                     /locus_tag="ACG8K3_001966"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9145821.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3456 a         2569 c         2212 g         4250 t
ORIGIN      
        1 aaagattccc actatcttaa gtattatagc aatatctaac tgtttttcct atgaaatcat
       61 tgttttttta tttaaaaact gaataatttt ttcttttttt aatttttaaa taaaagaaat
      121 ataaataaaa acagaggaag cggaatgatc cgcccctctg tttttatgat tttattaaac
      181 ttccaataat tctttttctt tatcagcagc gatcttatct acttctttaa tgctgttatc
      241 tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt
      301 tttttgttgt tttttatatt catcgatcgc atcacgacgg atgttacgaa tcgcgatttt
      361 tgcattttct gcttcttttt tcacatcttt agctaattct tttctgcgtt cttctgtcag
      421 ttgcgggata actaagcgaa tgacatttcc atcattggtt ggactgattc cgatatcact
      481 tgccataata gctttttcaa catcttgaag aatgctcttg tcaaaaggtg tgatcattaa
      541 aacacgtgct tctggaattt ggattgatgc catttggttc aatggtgttg gcgcaccgta
      601 gtagttcaca gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc
      661 aagttcacgt tgaagatttt gtgctgcttt tttcattttt tcttttgctt ctgtcataat
      721 tgcatctgcc atttttattt cccccttaca gttgttccga tattttcgcc taagattgca
      781 cggcgaatat ttccgtgttc atttaaattg aagacaagca gtggaatatc attatccatg
      841 cttagtgaac ttgcagttga atccatgact tgtaatcctt tggcaataac ttccagatga
      901 gtcagttctt caaattttac tgcatttgca tctactttcg gatcagcaga ataaacaccg
      961 tctacattgt ttttagccat caggataaca tctgcgccga tttctgccgc tcgtaatgct
     1021 gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt
     1081 cctttttcta aatgacgttc tgctttacgg cgaatatatg gctctgcgat ttgacgcatt
     1141 tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct
     1201 aaggcattca taactgtagc taacataccc atgtaatcag cttgagcacg ttccatgccc
     1261 atttgtgctc cgatttgtcc gcgccagata tttccgccac ccacaacgat tgccatttca
     1321 acgcccagct cgtgaacttc tttgatttct tggatgattt ctttgattac tggtggtttg
     1381 attccaaatc ctgcatcccc agctaaagct tctccactca attttaagac tacacgctga
     1441 tatttcggtg tgaccatgtt cattcctcca atattttcta ttgccatttt accatactta
     1501 gatgatttcg acactctaag actggttttt cttgttttgc ttaaaaaaag ggagcgcaca
     1561 aaccgtgcgt tcccgtctgt tattcaacag aattattttt tcacttggct cataacttct
     1621 tctacaaagt tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt
     1681 ttaacagttg cgccttttga tgcaacgtat ttttcaacag tcatatcagg atctttaacg
     1741 aatggttggt ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc ttctaccatt
     1801 ttttcaacga tattcgctgg tttgccttcg ttcaatgctt gttctgtaag aacagttttt
     1861 tcatgttcta attcagcttc tggaatttgt gattcgttta cataacgagg attgatagct
     1921 gctacgtgca tagctacgtc tcttgcaact gtttcgtcag ttgttccatc tagaacagtc
     1981 aatacggcaa tacgtccgcc catatgcaag tatccaccaa atgcagcatt gtcgtctttt
     2041 tctacaactt caaagcgacg gaagctgatt ttttctccga taacttgagt tgcttcgatc
     2101 aagtcagatt caattgtacc tttatctgtt ttgattttca ttgcagcttc catgtctgct
     2161 ggtttgtttt cagcaacaag ttctgcaatt tcttttacta aatcttggaa catttcgttt
     2221 ttagaaacaa agtctgtttc tgagtttact tcaacgattg ctgcagtgtt acctttcaca
     2281 gcaactgaag ccaatccttc agctgcaatg cggtcatttt tcttcgcagc ctttgccatt
     2341 cctttttcac gtaaaagatc aactgctttt tccatgtcgc cttctacttc aactaatgct
     2401 tttttcgcat ccatcattcc tacgccagtc atttcgcgta gttcttttac cattttagct
     2461 gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca
     2521 aaggagagtg aggcttgtcg cccagccttt gaaacagctc catcattcaa aattattctg
     2581 ctgaagaatt gtcgccttca acaacatcta cgatttcttc gattgatgtt gctgtgtttt
     2641 cttctgtgaa atcttcttca actacttgat cttcaccttg gtttccttcg atgaaagcat
     2701 cagccatttt tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt gaagggatta
     2761 ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat agctacgatt gggatattta
     2821 atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg tacattacat
     2881 ctgggattct tggcatatca gcgataccgc ctaagaattt ttcaagacgt tcacgttctt
     2941 tgttcaaacc agcaacttct tttttaggta gaacttcaaa agttccatct tcttccattg
     3001 cattgatttg tttcaaacgg ctgatacgtt tttggatagt atcccagttt gtcaatgttc
     3061 cgcctaacca acgatggtta acgaagtatt gtcctgcacg agttgcttct tctttgattg
     3121 cttcttgtgc ttgttttttt gtacctacga ataatgctac gccgccttct tcagcaacgc
     3181 tcttcatgta atcataagct gcatctacta atttaactgt tttttgcaag tcaatgatgt
     3241 agattccgtt tctttctgtg aagatatatt tcttcatttt tggattccag cgacgagttt
     3301 ggtgaccaaa gtgtacgccg gcttctagta attgtttcat agaaattact gccatgtttg
     3361 tttcctccga tttggtttta tttttcctct tcttatcttc taccttgccg gcaaactttt
     3421 tcaagcacct accgacaatc caataagaat gtggatttgc tgatgcataa agcaccctca
     3481 gctattatac agacaaaaga tccgtatcgc aagagcaaat atacttttct gctcaacatt
     3541 caatagttta cctgaaagat aaaaagaatt caagaaaaac agtagatttc tctttgtttc
     3601 tttattatcc tttttccata aaataatttt aggtataaaa gacgaatatt attgggcaat
     3661 ccttcttttt tttgttatga tacatgtgaa atctttcgta aaggagaaag aaaatgcgca
     3721 aagctcaaag acatttgctg attaaacaag tcattgaaga attcccgatc cgtacgcaag
     3781 aagaactatt aaacaaactt gggcattatg gcgttaccgc cacgcaggca acgatttctc
     3841 gagatatccg tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg
     3901 tattgtttcg cgaaaaagga actcctgaga ctaggactga ggaagaggat cggctggttc
     3961 aaatgattga agacattgta ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata
     4021 ccttgccaga caatgctcat ttatttgcat ctgttttaga tgagatcaaa cctccctcta
     4081 ttgtcagtac aattgctgga ttcgatacga tcatggtcat ctcggaaaat gaggaggacg
     4141 caaaacgagt agaagaattt cttcaccatc caactgaagt atcacttttt taatcagagc
     4201 acgtattcct aatatggaat gcgtgttttt ttattttcaa tattttcctt attaaataaa
     4261 cctttacact ctttacaaaa atacatcatt ttttattcaa ttttttcaac accttgattg
     4321 aatcacaata atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt
     4381 atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt attaactcct ggaccattga
     4441 cgacttctga aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat
     4501 acaaagaaat tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag
     4561 gaatatacac aacggtctta atgcaaggta gcggctcctt tggggtagaa tcggtattaa
     4621 gttctgtgat caggccagaa gatactgtac tgatttgttc aaacggcgca tatggacaac
     4681 gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcggaac
     4741 atcaaatcct ccaagctcat gtgattacac agctgattga agagaatccc tctatctccg
     4801 cactagcaat ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg
     4861 ctgctgtcac acaagaaaaa gatcttttgt ttatagtgga cgcgatgtca agctttggtg
     4921 ggataccgat tcgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat
     4981 gtattcaagg tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctagaaaagc
     5041 gaaaaaatgt gagtcgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa
     5101 acgagaatgg caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag
     5161 cgctgaagga actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca
     5221 ataatcatgt actacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat
     5281 atcaggggcc attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc
     5341 atttttatca gtatttaaag caaaacggat ttgtgatcta tcccgggaaa atcagcgatc
     5401 atgaatgttt tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga
     5461 cagcaattat caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg
     5521 gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt
     5581 ctagaaaaag gaatccaact gactgacaaa gaaatccgcg aaccgatggg aaaattaaaa
     5641 tgggatcata tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt
     5701 tatggacaac tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat
     5761 ctgtttaaag atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa
     5821 cggttgagaa acgctgggat caatattggt tccacgactg gatttactgc tcctatgatg
     5881 acaatcgtag cagaaaccgc aaaaaaagaa gggtattcac cagacttcat ggcaacttct
     5941 gaagacgtcg aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac
     6001 ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag
     6061 gaagggaaaa atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg
     6121 atttcttatg atgagtacca aaaattagag atggggaaaa aagatcgttt aaaacagtct
     6181 gttgctctta gattccaaca agctggcgct gatatttgtg taaacaattt aaaagagtta
     6241 acagaataca ttttgtacgc tgacaagaat ccagaatgac ttttatacaa aatcagaggt
     6301 cgtgaaaaag ctgtactgca tcaagtaata agaacagcca aacaaaaata gctcctcata
     6361 tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
     6421 aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc ttatttacaa acaaacagtg
     6481 tttaagagac aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca
     6541 aaaaacgata aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa
     6601 cgctgatttc acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg
     6661 aaggtcttct ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg
     6721 ttcatcatca gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt
     6781 gattgcatgt ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc
     6841 agggtatttt tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc
     6901 tgctgtttct gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt
     6961 atattgcaca taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc
     7021 aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg
     7081 tttagctaca gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc
     7141 agattggatt gcttcattta atacttcttc taataagacg atctttcctt tacgagttga
     7201 taatttttta ccaccttgcg tgatcaaacc aaatggaata tggcgcatat cgtcagacca
     7261 gtcaaagccc atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt
     7321 ccctactaca tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc
     7381 taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag
     7441 gtcataagca gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc
     7501 ttctaaaagt ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga
     7561 atcgaaacgt acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg
     7621 gaaccattgc cataattgaa tagcctcttc atcgccttct tccagacgtt tgaaccaagc
     7681 acgagcttct tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacatataa
     7741 acgaagcaat tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta
     7801 agcgacgatc agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg
     7861 ctgataaccg attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa
     7921 atgtcccatt gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc
     7981 ttgattcccg atattgctat ccccataatg ttctttttct ttcactactg tttgcaaaac
     8041 ttttttgctg ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat
     8101 tttttcaaag ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc
     8161 ttttcggtaa acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga
     8221 ttttggattt tctagtaatt gttcgacttg ttccagtgtt aaatcatctt tgacagcgtc
     8281 atagacagct tttgcgacaa gatctttatt attcatcttt attcctccta aagtgctccc
     8341 ttgattgtta aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga
     8401 cgagatttcc cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc
     8461 ggtagacgac cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca
     8521 ttcggcttcc accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca
     8581 acaaatctta gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag
     8641 tcgtttctga taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
     8701 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
     8761 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
     8821 ttggttttgc ttcaacaaat gcaatagctg cctctacttt cggaagcatg cttcctggag
     8881 caaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
     8941 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
     9001 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
     9061 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
     9121 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
     9181 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
     9241 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
     9301 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
     9361 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcattt tgtaaccagt
     9421 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
     9481 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
     9541 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
     9601 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gattgcatta ccccctaaag
     9661 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
     9721 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
     9781 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
     9841 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
     9901 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
     9961 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    10021 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    10081 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    10141 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    10201 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    10261 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    10321 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    10381 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    10441 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    10501 cgattccatc aaacatacga cctagtactt tagctgtatc ttctgtggat tcttttttac
    10561 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    10621 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    10681 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    10741 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    10801 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    10861 gaggaaatcc cgacggcagc tgacagtaag accgccgtca ggaaacaaat tacttcaagt
    10921 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acggcctcgt gacaattcac
    10981 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    11041 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    11101 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    11161 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    11221 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    11281 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    11341 cacggttgtt tggaattttg attgctaaca ctttttcaaa tcctgaattt tttgcgaata
    11401 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    11461 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    11521 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    11581 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    11641 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    11701 gatagtcatc gtctgctgac agatcgtata gactgctaga acgtacttca atatcttttg
    11761 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    11821 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    11881 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    11941 gagtctcgac gccttcattt tgcaatgtct ttgcaaagtt gtcatgttct tcttgagcaa
    12001 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    12061 ccacttcttg tccaggcctt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    12121 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    12181 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    12241 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt tattcatttt
    12301 ttattctgta tacactttca aaaaaatatt tattcattga aaaaaatttt ttgaataaat
    12361 taaaaaaata aattatatta gcgttgttag cgtttactat atgtattatt gttgtttttt
    12421 atacacaaat cattcgcttc aaaacaaatt attcaatacc attgtatatt ttttacataa
    12481 taacaaa
//
LOCUS       DAZKCN010000074        12214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000074 DAZKCN010000000
VERSION     DAZKCN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.076"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..340)
                     /locus_tag="ACG8K3_001967"
     CDS             complement(176..340)
                     /locus_tag="ACG8K3_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145822.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            635..1963
                     /locus_tag="ACG8K3_001968"
     CDS             635..1963
                     /locus_tag="ACG8K3_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP9145823.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2165..2998
                     /locus_tag="ACG8K3_001969"
     CDS             2165..2998
                     /locus_tag="ACG8K3_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9145824.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3014..3751
                     /locus_tag="ACG8K3_001970"
     CDS             3014..3751
                     /locus_tag="ACG8K3_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP9145825.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3906..4874)
                     /locus_tag="ACG8K3_001971"
     CDS             complement(3906..4874)
                     /locus_tag="ACG8K3_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9145826.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5106..5939
                     /locus_tag="ACG8K3_001972"
     CDS             5106..5939
                     /locus_tag="ACG8K3_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9145827.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5973..6812
                     /locus_tag="ACG8K3_001973"
     CDS             5973..6812
                     /locus_tag="ACG8K3_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9145828.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6838..7398
                     /locus_tag="ACG8K3_001974"
     CDS             6838..7398
                     /locus_tag="ACG8K3_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9145829.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7597..9318
                     /locus_tag="ACG8K3_001975"
     CDS             7597..9318
                     /locus_tag="ACG8K3_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP9145830.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9483..10625
                     /locus_tag="ACG8K3_001976"
     CDS             9483..10625
                     /locus_tag="ACG8K3_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP9145831.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10641..11852
                     /gene="thiI"
                     /locus_tag="ACG8K3_001977"
     CDS             10641..11852
                     /gene="thiI"
                     /locus_tag="ACG8K3_001977"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9145832.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4159 a         2050 c         2538 g         3467 t
ORIGIN      
        1 taatcatgat gtcgtaggtt cgagtcctac tgccggagta aataaagctt atgcaaagaa
       61 aaagaaatcc aatcacttgg gtttcttttt cttttattat tatgatgaat caagctttct
      121 gtttttgtca cgtatgttgt aatctttacg aaagagtaga gttggctact cataatcaac
      181 catacttccc atcatcgcta aatacgaaat aaatcagcat tcctacacca atcaaagcac
      241 aagctgcacc aagaaaaggg atgacattcc caaagttatc tgtattcata agtatgccac
      301 cacccacaaa caaaaagccg ccagcaatca ggtttttcat tttattcatc tcctttctta
      361 cgttatagta tttccttctt tcaatgagta ttttgaaagc tatcttattg tatgatgttc
      421 ttctaggcct cttctttatt atacaggcca ttttcgttat ctcatagaaa tattcctttt
      481 tatctgaaaa ctattgtata ccgtatgaca tttttcttca tgaagttgta agtatattct
      541 cattcaaatg aaaatttcat catagaagag attgtatttc taaattctta agagtaaaat
      601 acgaagtata acttggattt ggaaggatga ttggatgaaa gctattacgc ctgatatgac
      661 aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag caggcattgc agcgggcagt
      721 tgtgaagaac ggaattactg cttctgctga aaatgtttca gcacatgtgg agaatacacc
      781 ggtattttct atagatttgg ctacggggaa agtggctaat caaatgcaat cggggcgttg
      841 ctggatgttt gctgcactga acacattccg tcataagatg ctgaatcaat tccatttaaa
      901 agagtttgaa ttatcacaaa attacacttt tttctgggat aaatatgaaa aggcaaatta
      961 tttctatgaa aacatcattg caacaggaaa agaggcaatc gatagccgga aagtagcttt
     1021 cttactcgct actcctcaac aagatggcgg ccaatgggat atgatcgttt cattattcca
     1081 aaaatatggc gttgtgccta aatcggtgat gccggaaagt gcgaatagtt caaattctcg
     1141 agatttgaat aactacttga acaaattact gcgtaaagat gcagtagtgt tacggaaaat
     1201 ggttgctcaa ggagaaacat tagacgctat tgaagaaaag aaagaagaaa tgctagaaag
     1261 catttataac ttcttagcta tctcattagg aacacctcca aaagaattcg attttgagta
     1321 ccgcgatgaa gaaaagaact atcatcttga ccgaggactg acaccacaaa ttttttatga
     1381 taaatacatc ggtgtaaaat tagatgatta tgttagcgtg atcaatgcac caacaaaaga
     1441 caaaccattc aatcgttctt atacagtaga gatgctagga aatgtcgttg gcgggaaaga
     1501 agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa ttagcgattg ctcagcttga
     1561 agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa tcttctacta gaactagtgg
     1621 catcatggcc ttagatgctt attcaatgga tgatctattt gatacggatt ttacaatgac
     1681 taaagctgaa cgtttagatt ttggagaaag tctgatgacc catgcgatgg tattgaccgg
     1741 tgttgactta gtagacgggc aatcaacaaa atggaaagtc gaaaatagct ggggcgaaaa
     1801 agttggaaag aatggcttct ttgttatgag tgatgcctgg atggacgaat atacgtacca
     1861 aatcgttgta agaaaagatt tattgacgaa agagcaatta aatgcgttca atgaagaacc
     1921 aattgttcta tctccgtggg atccaatggg tgctttagca tagacgagta tttcaatgaa
     1981 gagtatgtag taaagataaa aagagctggc ttaagccagc tctttttatc tttaaagtcc
     2041 attccctgtt ttttatggtc cctgtgctat aattctaaat aaaggataag gattggaaat
     2101 aatcctttta tcattaaaaa tatgatgacg aagtagaata aaattgggag taggggggaa
     2161 agtcatggat atccaaactg ttttatctga tctgaaatta aatggtaaag tgattccagt
     2221 tgttggtggc gatgttaatc agacctatcg tataaaaacc gaacatcgtg cttatttttt
     2281 aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca gaagtagacg gattaaaaga
     2341 actgtcagca ttcgtacgtg taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc
     2401 ttatttgtta atggaatgga tcgagccggg aaaaggggat caacgagatc ttgctgcagc
     2461 tttagctaat ctccatcagc aaacagcgcc acaatttggt tttcgtaaag ataactattt
     2521 gggtacactt gttcagaaaa attcttttga agaagattgg tggacttttt tctttaaaga
     2581 tcggcttgaa tcccagattt ctttggcaga agaaacgaat cgatggaatg tacagcgtca
     2641 agaaaagtat ctgcgattta aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac
     2701 tccacgcctc ttgcacggag acctgtggag tggtaatgtg ttctttgacc agcaagggca
     2761 tccagttttt gtcgacccag ctgtttctta tgggaatcgg gaacaagata ttgcaatgag
     2821 ccagttattc ggtggtttcc gaccggaatt tttagatgct tatcaaacta tatttccatt
     2881 agaaaaggga tggaaagacc gactgccgat ttaccagtta tattatcttt tggcacattt
     2941 gaatatgttt ggagaaagtt acggttccca agtagatcaa ttactagaaa atttttaaga
     3001 ggagggcgtg tttttggcaa aaaaatatac caaaaagcat gaagtttcat attatgagtg
     3061 tgacatcaac caaacgatga catttccttc gatgttagga gtagttatca aaacgtctga
     3121 agatcaaagc gaccagttgg gaagaggcag tgaattcgtc aatagctttg gacttacttg
     3181 ggtcatcacg aattattcca tggagattaa tcgtctccct agagtcggtg aagtaatctc
     3241 agtaacgact caagccatgg agtacaataa gtttttctgt tatcggaact tctggatcca
     3301 tgatgaagat gggaaagaat tagtcaaaat agaatctgtt ttcgtattga tggattttgt
     3361 gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct ccttttgaga gtgagaagat
     3421 caagaaaatc aagcgtcagg aaaaaattga aaagattgat tcgggcttca tgttgccata
     3481 tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt aataatgcga tgtattttaa
     3541 ttggatcatt gacgtattag gatatgactt tttgactacg catgttccag aaaaagtaat
     3601 tattcgattt gataaagaag tagagtacgg gaatgagatc gaaagccatt ttgaacaaat
     3661 ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga atgcaagaac agctgtattg
     3721 tgaagcaaat attatatgga aaaataaata aaaaagaggt tgtgacagaa gggggcaact
     3781 tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt
     3841 agttttcgaa gaagctattt ttgtcacagc ctctcttttt aagtttagct attagataat
     3901 gatgtttagt tttggataaa cgaaatcaat tcttcacccg acaaaccata gtttacagca
     3961 atcaatagtt ttaaacgtgc tttttgacta ttaatactgt tacagaaaat aatccccatt
     4021 ttttctagtt cttttccccc gccttcataa tcataaacag gctctgcgat tccattgaaa
     4081 caacgagaaa caagtactac agggattttt tggacaagaa gcttttccaa agcagctaag
     4141 gcttgtggag ggagattccc cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta
     4201 tgggcgatgg cttccaataa gtccccttgc atcccagcat atgctttaac gatcgggata
     4261 gtcccatcta cagcggaaat gtccagtcgc tttgtttcta aaagttcctg caagaataaa
     4321 attcgattct tagttactaa gccgatggga cccaatgtag gtgttcggaa agtagcaaca
     4381 tttgtggtat gggtctttgt cacgtagcgt gcagaatgga tctcatcgtt catgaccacc
     4441 aaaaccccct tatctagcgc ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta
     4501 taaaggccgt cacttccaag ttcattactt gaacgcattg ccccagtcaa aacaattggt
     4561 aatttgttgc caatcgttgt atcaagaaaa aaagcagttt cttctaatgt atctgtaccg
     4621 tgtgtgatca ccactccgga tatacctgat gcataagcgg acaagatacg ttcttttaat
     4681 accaacatgt gagaaggcat gatgtgtggt gagggaagat tgaagatatc ttcaactaat
     4741 aattctacat gcttggggat tgtgatttct gcatctaata atgggtttgc cacatcagga
     4801 ctaacaccgc ccgtgagcat gttttctttc atggcgatcg ttccgccggt gtgtagtaca
     4861 agtatttttt tcattttaca gacttcctta cgaataattt tgcatagccg ttaaatctta
     4921 ttgtatacta tataaaagag ttttgctatc agtgaagaat aacgaatagc ctaacttatc
     4981 ataaaaaagc tgaccttatt aaaatggaaa agactcaacc ttgacctcct aatacaatgg
     5041 agtatctggt tgagtttctt ttacaagaaa gcagccaagg aaatcaatca cgaaggtggc
     5101 cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca tgacaaatta actggtaaaa
     5161 agaataacac tctttatgaa tatgcagggt ttctaccctt agtggaatta gatactgcct
     5221 attatggaat tccccgaaag tcctcagttg agaattggat aagtgaagta cctgaatcat
     5281 ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca aggagactgg aagccttatt
     5341 atcagaccga agaagagatg attgcgcatt ttttagatgt tatgtcgcca atggtagatg
     5401 ctggaaagat gttttgcttt ttagtccaat tcccctcatc attcaaatgt acgaaagaaa
     5461 atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg ttatcctgtt tctatagaat
     5521 tgcgggatta ttcttggtac agcgatgaat ttcttgaacg tacaagaaac tttatgtcta
     5581 gagccgcttt ttctttagcc atcatcgatg agccacagtt gctgaataca acgattcctt
     5641 ttgatccatt tgtcaccaat ccgaactttg ctctttttcg attccatggt aggaatgcgc
     5701 aaggatggca agcaaatgat aaagagtggc gtaaaaaaag aacgttatat cgttattcag
     5761 aaaaagaact gcaagaacta gcacatgaag tcaaagtagt tgattctaag acaaaagagg
     5821 taggtgtgat ttttaataat aattctggtg gtgatgcggc tgaaaatgca ttagcactgc
     5881 aaaaaatatt aaatatcaag ccagatggac tcaatccaac ccaaatggat ctgttttaag
     5941 ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa agctattttt tttgatattg
     6001 acggaacatt ggtcaccaat cgttcaaaag cgttggaatc aacaaaacaa gcaattgaat
     6061 atgctcggaa aaacggcatt ttatgcggtg tagcaacggg gcgcagccca gtaaagatca
     6121 aagaaatcat tgatgaattg gaattagata tgtatgtcgt gtataatggt caactggttt
     6181 ttactgcaga tcgcacgatt atcgaccacc cttttgaaca aaaagtattg gaacatattg
     6241 ttgaatttgc agatgagaac catcgacaaa tcgtttttgg tgcgagaaat cggttagatg
     6301 gatcaacgac gatgctatta ggacaatcga tttttatcaa gcgactagtc agctttttac
     6361 cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca ggtttttagc cctcaccgac
     6421 aaaaggatcg ttatgaaacg ttagagatat taaaagaacc aatttatcaa tgtatattgc
     6481 tcagtccgga gtcagaacag aaaaagttag aacatcgctt tcccgagtgt acgtttcagc
     6541 gttcaaatac gtatactgta gatatcatcc caaaaggtgg ttcaaaatta ctaggcatcc
     6601 aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat tatggcattc ggcgatcatt
     6661 acaatgatat cgagatgctg aaaggtgtag gaattggagt ggccatgggg aatgctcaga
     6721 tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa tgaagaagat gggatatacg
     6781 aaggactgaa acacttcggt ctgatttatt agtcaatgaa agaaggaaaa aatcagtatg
     6841 gaagcagaga caccttttca aaaagtagaa acattccaca aaacttttga tccaagaaga
     6901 ccagagatac ccacggcttt tacagaaaat gaagctcata cacgtgctgc gttcaaaata
     6961 gaagaacttg ttgaatttgt tgcagctact tcacctaata aagaacaact gcatgaagct
     7021 gttatgctgc tgcacgaagc attagataaa gcagaagaaa aaatacttgg taaaacacag
     7081 tggggaagtg atccgctggt tgaacaagta gatgcactga cagatatcct ttactttact
     7141 tatggttctt tttcattact aggaatagtg cctgatcgaa tattcgaaat cgtccatcag
     7201 gcaaatatgg ggaaattatt tccagatgga aaacctcatt atgaccctgt aacaaataaa
     7261 gtgttgaaac ctgaaaactg gcagatggaa tatgctccag aacctaaaat cgcggcagaa
     7321 atccaacgtc agattcaaga agcgattgct aagaagaaat ccgctgatat aggcatgtgc
     7381 acaaatgaaa aagagtgatc agagtttcac tatttttgtt atctttatac gatggaaaaa
     7441 caaaagattt tcgtttaagt tagtgaatgc atgaaaatag tatgctataa tctagaatag
     7501 atgtcttttg cacgagagac gtattatttg gatttatgcc aagcagaacg ccatctgttg
     7561 cataaaagat gcatactgaa aacggaggaa ccataaatgg gaaataatgt tatcatcgga
     7621 atcgtcattg caatcattat agtcgccatt gtcgtctttt tggttagtta cttgatgaag
     7681 aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca aggaagaatt atttgacttg
     7741 ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc tggttgggca aagccaaaat
     7801 acctttcgtg aatggagtca aaaatggaat gacatatcta cttcctcgtt cgctgaacta
     7861 gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt ttcggttctt aaaaggaaaa
     7921 aaagcagttg ttgaagcaga agctacaatg gaccaaatgg agaaacaagt gacggaaatc
     7981 cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca actcactagc tgttcagcag
     8041 gcacttgacg tctatgaaga actgaaagaa aaagtcagag aagacgagca tgaatacgga
     8101 ccagctcttt ttgaattgca aaaacagatc aaagtgatcg aaaaagaatt tacgcagttt
     8161 gtgactttaa acacttctgg agaccctgtt gaagcccgcg aggttttgga taaagcagaa
     8221 aaacatacgt atgaagtgga agctctgatg aaaaaaattc caccgctgta tgaggagctc
     8281 cataccactt tccctgaaca acttgaagaa atcagtgata cttacgagaa attgatggaa
     8341 gaacaatatg tattcccaga agaagatctt gctgaagata tcgcaaaagt aagtcgaaga
     8401 attgaaaatt cattagccaa tcttgaaaaa acagaagttg aaacggtcga atttgaaaac
     8461 cgtgaaacag ctgatttgat tgattcgtta tatgatatct tagaacggga aatggaagct
     8521 cagagatatg taaaaactaa ccaatcaacg attgcagagt atatcaaaca tacaacaaag
     8581 aataaccgtc aattattgat tgagctggat cacacatctc aaagctatac attgaaccac
     8641 aatgaaattg gccgtgtacg aggtttccaa accgaagtgg aagaaatgga acgtcaaaat
     8701 gaacagatga ttccacagat ccgtcaacac gaaattcctt attcagaagt gcgcacgttc
     8761 tataaaacag tcttcaaagt cttagaagat atcgaaaagc aacaagtaga gatcgacgac
     8821 tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag aaaagatcga tacattcgaa
     8881 ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac gtttgcctgg cttgccgaat
     8941 gattacctag aattcttttt tgttgctact gaccgtattg aagaactaag tgttgtcttg
     9001 aataaaattc gagtaaacat ggaagaggtc aatcgtttag ttgctctatg tgaagaagat
     9061 ctagaattat tagataagaa gacacatgat ttagtagatg cagcagcatt aacggagcaa
     9121 atgctccaat atgcaaatcg ttatcgtcat acacacgaag aagtgagaga agcaattgat
     9181 aaaagctact atttattcaa taaagaatat cactatcaag aagcattaga cgaaatcggt
     9241 acagcattag agcgagtaga acctggtgct ttcaaacgca tcgaagattt ttattttaat
     9301 catccagact tagtataaag caaaaaagtt agtaaaccag caagagcaag acggattatt
     9361 gtcttgttct tgtctttttt tgtatatttg aaaattgaaa caagtgaaaa tttcacctgc
     9421 tcttgaaagc accttggaca accgttataa tagaaaagaa tttcaaaaaa gaggggtttc
     9481 ttatgatata ttttgataac agtgcaacaa ccccaatctt tccgcaaagc ttagatgcat
     9541 atgtaaaaac cagtcagcgg attattggga atccatcaag cttgcatgat ttaggcaatc
     9601 aagctaaccg gttgttgcag caagcaagaa agcagatcgc tgatttgatc catgtagaac
     9661 cagaagaaat ctattttact tcaggcggaa cagaaggaga taactgggta ttaaaaggaa
     9721 cgatgatcga aaaaagagaa tatgggaatc atatgatcat ttcaggagtc gaacatccgg
     9781 ccgtcagcga gacagcagaa caattaaaag aattaggaat cgaagtaagt atcgccccag
     9841 tggataaacg cggatttgta cgtgtcgacg aattgaaaga attaataaga aaagagacag
     9901 tattagtctc tgttatggct gtgaataatg aagtaggatc gattcagccc atccaggaaa
     9961 tcagtgatct tttggatgat tttcctaaaa ttcatttcca tgtagatgct gttcaggcga
    10021 ttggaaaagt accattctcg caatggttga cagaaagagt tgattttgca acattttcag
    10081 ctcataaatt ccatggccca cggggaactg gtttcattta ttggaaaaag ggtcgtcgtt
    10141 tagcaccatt actgacaggt ggagggcaag aaagaaatca gcgaagtggg acagaaaatg
    10201 ttccaggtat tgtggctatg gttaaagctt tacgtttatc attagagata aaggaacaaa
    10261 aacctgagca gacacaacgc ttaaaagtag ctctaatcaa tgcgttgaag acgtatgaga
    10321 aagtgacgat tttttcagaa ggaactgact atgcaccgca tattttatgt tttgctctaa
    10381 aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga aaaacaaata tttgtttcta
    10441 ctactagtgc ctgctcctca cgtaaacata tggcaagcag tacattgcac gcaatgcatg
    10501 taccaaatga tctggctaca tctgctgttc gaatcagctt agatgaaaac aattcgcttg
    10561 cagaagtcga gcaatttatg attattttca agcagcttta taagaaattt tctgcaatca
    10621 attaaaagag gagtgttctt gtgaaataca gtgaaattat ggtccgttat ggggaacttt
    10681 cgacaaaagg aaagaatcga aaaagcttta tcatgcaact agcacaaaat gtcagacaag
    10741 ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg ggaccggatg catcttttat
    10801 tgaatggcgc agacgggcag ctagttattc caaaactagc aaaaatattt gggatccaaa
    10861 atttctctcc aagtattcgt gtagaaaaag atgtcgaagt tttgaaacaa gctgttcaag
    10921 acatcatgaa agaaatttat actgggaaag aaacatttaa aattatcgct aaaagaagcg
    10981 atcaccaatt tgaattgaca agtaatgaat tgaatcaaac attaggtaat gctgtttttg
    11041 atattttccc gcatatccaa gttcaaatgc gtcaaccaga tattccatta cgtgtagaga
    11101 ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa gggagctgga ggattgcccg
    11161 taggaacaag cggaaaagga atgcttatgc tttctggggg gattgattct cctgtagcag
    11221 ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt tcattttgct agtccgccgt
    11281 atacaagtga acaagcactg caaaaagcaa aagatctaac agccaaacta gcaccttatg
    11341 tgggaacgat ccattttatc gaagtgccat ttaccgagat tcaagaagaa atcaaaaagt
    11401 cagtgccaca aggatattgg atgacaatta ctcgtcgaat gatgttgcgt ttgaccgatg
    11461 aaattcgccg tattcgtcat ggtttagtca ttttgaatgg tgaatcatta ggtcaagtgg
    11521 cttcacaaac tttgcaaagt atggtggcta tcaatgaagt aacgaatacg ccaatcattc
    11581 ggcctgtagc gacaatggat aaacttgaaa tcattgaagt agcagaacag atcgatacat
    11641 ttaatctttc tatccagcct tttgaagatt gctgtacgat ttttgctcct ccacagccaa
    11701 aaacacgccc tcaactggaa aaggtccatc aatatgaaga gcgtttagca atagatgaaa
    11761 tgatccaacg agcactagct ggcttgaaga tggaagagat acaagcagaa tcgtctaatg
    11821 aaaaaactga agaattttca gaattgctat aagaggttat gaaaatagtg ttttgaccag
    11881 agtattagag aaacaaaacg aaaaaatagc tccatgtatt ttaccgtttt tgaactaatg
    11941 tcgaagatta gctgattgaa taccatttat caagaagtta tggaaaagcc ggttcttttt
    12001 tactaactga aaaataaaat tttatttaga gatcaagcta cagttgataa atgatataaa
    12061 aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag tgggcagctt caagaaataa
    12121 gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa tttcggctta tttttcgaag
    12181 aagttacttc tggaacaccg tgtatcagag gttg
//
LOCUS       DAZKCN010000075        11681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000075 DAZKCN010000000
VERSION     DAZKCN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.077"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1450
                     /gene="lysA"
                     /locus_tag="ACG8K3_001978"
     CDS             191..1450
                     /gene="lysA"
                     /locus_tag="ACG8K3_001978"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9145833.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1437..2237
                     /gene="dapF"
                     /locus_tag="ACG8K3_001979"
     CDS             1437..2237
                     /gene="dapF"
                     /locus_tag="ACG8K3_001979"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9145834.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2240..3121
                     /gene="dapA"
                     /locus_tag="ACG8K3_001980"
     CDS             2240..3121
                     /gene="dapA"
                     /locus_tag="ACG8K3_001980"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9145835.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3118..3834
                     /gene="dapB"
                     /locus_tag="ACG8K3_001981"
     CDS             3118..3834
                     /gene="dapB"
                     /locus_tag="ACG8K3_001981"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9145836.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3840..4535
                     /gene="dapD"
                     /locus_tag="ACG8K3_001982"
     CDS             3840..4535
                     /gene="dapD"
                     /locus_tag="ACG8K3_001982"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP9145837.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4551..5552
                     /locus_tag="ACG8K3_001983"
     CDS             4551..5552
                     /locus_tag="ACG8K3_001983"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9145838.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5549..6910
                     /locus_tag="ACG8K3_001984"
     CDS             5549..6910
                     /locus_tag="ACG8K3_001984"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP9145839.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6870..8021
                     /locus_tag="ACG8K3_001985"
     CDS             6870..8021
                     /locus_tag="ACG8K3_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP9145840.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7996..9165
                     /locus_tag="ACG8K3_001986"
     CDS             7996..9165
                     /locus_tag="ACG8K3_001986"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9145841.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9340..10719
                     /locus_tag="ACG8K3_001987"
     CDS             9340..10719
                     /locus_tag="ACG8K3_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9145842.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11000..11681
                     /locus_tag="ACG8K3_001988"
                     /pseudo
     CDS             11000..11681
                     /locus_tag="ACG8K3_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         3888 a         2034 c         2597 g         3162 t
ORIGIN      
        1 gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt
       61 ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca
      121 ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag
      181 gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa
      241 gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt
      301 caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt
      361 ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag
      421 tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca
      481 tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat
      541 tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa
      601 aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat
      661 cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca
      721 agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat
      781 cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta
      841 cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta
      901 tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac
      961 ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag
     1021 aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc
     1081 acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta
     1141 tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt
     1201 gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc
     1261 gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat
     1321 gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc
     1381 aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga
     1441 tccttcataa atataatggc tgcggaaatg attttatctt attagattac gcagaagaca
     1501 cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat
     1561 tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg
     1621 cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga
     1681 tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg
     1741 atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa
     1801 tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag
     1861 taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc
     1921 gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa
     1981 aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg
     2041 aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg
     2101 ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact
     2161 tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa
     2221 gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa
     2281 tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga
     2341 acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc
     2401 tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat
     2461 tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc
     2521 tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg
     2581 actagctgct cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa
     2641 tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca
     2701 tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc
     2761 tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt
     2821 aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt
     2881 ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt
     2941 aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc
     3001 agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc
     3061 aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg
     3121 aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa
     3181 agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt
     3241 acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt
     3301 tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca
     3361 cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt
     3421 tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc
     3481 tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact
     3541 gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg
     3601 caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta
     3661 cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa
     3721 tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat
     3781 cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg
     3841 tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag
     3901 tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa
     3961 gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag
     4021 aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag
     4081 atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa
     4141 tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg
     4201 aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact
     4261 gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg
     4321 ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata
     4381 tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa
     4441 ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa
     4501 atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa
     4561 tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag
     4621 aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta
     4681 aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg
     4741 aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa
     4801 tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg
     4861 atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg
     4921 caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct
     4981 ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag
     5041 gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata
     5101 ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag
     5161 aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag
     5221 caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc
     5281 aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat
     5341 tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata
     5401 tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata
     5461 cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt
     5521 taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg
     5581 cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga
     5641 tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa
     5701 agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc
     5761 cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt
     5821 acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga
     5881 agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg
     5941 ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca
     6001 ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc
     6061 agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga
     6121 cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga
     6181 tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga
     6241 aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc
     6301 agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc
     6361 tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg
     6421 aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga
     6481 aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca
     6541 tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa
     6601 gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat
     6661 cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc
     6721 tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg
     6781 agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa
     6841 gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc
     6901 aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa
     6961 aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata
     7021 atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag
     7081 aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg
     7141 gacatatggc gatgctttta gggttgactg actttataac taaacacatt gaggatctta
     7201 tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg
     7261 ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat
     7321 ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca
     7381 gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa
     7441 tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat
     7501 taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa
     7561 atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc
     7621 aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta
     7681 tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag
     7741 cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt
     7801 ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc
     7861 ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat
     7921 ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag
     7981 aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata
     8041 gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa
     8101 ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca
     8161 gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa
     8221 gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt
     8281 ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa
     8341 ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta
     8401 gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa
     8461 caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat
     8521 cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt
     8581 ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt
     8641 ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta
     8701 tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt
     8761 attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa
     8821 aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa
     8881 tatcaaacac gaagagatta tctggtccag caattggcgc ctttaggatt tgaagtgacc
     8941 cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct
     9001 tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca
     9061 tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta
     9121 catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc
     9181 aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt
     9241 agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt
     9301 tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc
     9361 attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt
     9421 gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt
     9481 atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca
     9541 gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat
     9601 gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct
     9661 tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca
     9721 gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa
     9781 ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa
     9841 gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga
     9901 cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa
     9961 aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt
    10021 gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt
    10081 gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc
    10141 caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca
    10201 cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt
    10261 agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc
    10321 ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg
    10381 aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg
    10441 ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc
    10501 tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca
    10561 ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca
    10621 gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc
    10681 gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag
    10741 aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct
    10801 tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10861 ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat
    10921 ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga
    10981 tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat
    11041 tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac
    11101 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa
    11161 gaagctagga ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg
    11221 aggaaaagtt ggagcagtag ccaaaaatcg gatccatcgt cgtttctaca ccagtatctg
    11281 ccatcagaaa atcacgacgg atacaactga atttaaatac catgaagtgg atgccaaagg
    11341 cattatccgt caaaagaaat tgtatctaga tccttttatg gatatgttca atagtgagat
    11401 tttatcctat cgtatctctg aaaagcccaa tgcattagcg gtaatggaag gtttggagga
    11461 agctatccag accactaacg attgtcccta tcgtcgtacg tttcactcag atcaaggttg
    11521 ggcgtatcaa atgaaagctt atagaaacaa attgaaggag tacaaaatct ttcaaagcat
    11581 gtctcggaaa gggaactgct tagataattc acttatggag aatttttttg gcttattaaa
    11641 acaagagatt tttcacggga aagtttataa ctgtttcgag g
//
LOCUS       DAZKCN010000076        11611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000076 DAZKCN010000000
VERSION     DAZKCN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.078"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8K3_001989"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACG8K3_001989"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9145843.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACG8K3_001990"
     CDS             complement(2262..2513)
                     /locus_tag="ACG8K3_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP9145844.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACG8K3_001991"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACG8K3_001991"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9145845.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACG8K3_001992"
     CDS             3463..3681
                     /locus_tag="ACG8K3_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145846.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="ACG8K3_001993"
     CDS             complement(3970..4482)
                     /locus_tag="ACG8K3_001993"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9145847.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACG8K3_001994"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="ACG8K3_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9145848.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="ACG8K3_001995"
     CDS             complement(6935..7378)
                     /locus_tag="ACG8K3_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP9145849.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="ACG8K3_001996"
     CDS             complement(7379..8167)
                     /locus_tag="ACG8K3_001996"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9145850.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACG8K3_001997"
     CDS             complement(8164..9105)
                     /gene="rnz"
                     /locus_tag="ACG8K3_001997"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP9145851.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="ACG8K3_001998"
     CDS             complement(9282..9869)
                     /locus_tag="ACG8K3_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP9145852.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACG8K3_001999"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="ACG8K3_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP9145853.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         3155 a         2632 c         2037 g         3787 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc g
//
LOCUS       DAZKCN010000077        11584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000077 DAZKCN010000000
VERSION     DAZKCN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.079"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1928
                     /locus_tag="ACG8K3_002000"
     CDS             45..1928
                     /locus_tag="ACG8K3_002000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9145854.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2003..2287)
                     /locus_tag="ACG8K3_002001"
     CDS             complement(2003..2287)
                     /locus_tag="ACG8K3_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9145855.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2277..3011)
                     /locus_tag="ACG8K3_002002"
     CDS             complement(2277..3011)
                     /locus_tag="ACG8K3_002002"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP9145856.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3140..3763
                     /locus_tag="ACG8K3_002003"
     CDS             3140..3763
                     /locus_tag="ACG8K3_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP9145857.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3955..5076
                     /locus_tag="ACG8K3_002004"
     CDS             3955..5076
                     /locus_tag="ACG8K3_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9145858.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5083..8211
                     /locus_tag="ACG8K3_002005"
     CDS             5083..8211
                     /locus_tag="ACG8K3_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9145859.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8230..11424
                     /locus_tag="ACG8K3_002006"
     CDS             8230..11424
                     /locus_tag="ACG8K3_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP9145860.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLQELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
BASE COUNT         4000 a         2036 c         2475 g         3073 t
ORIGIN      
        1 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
       61 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
      121 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
      181 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
      241 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
      301 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
      361 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
      421 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
      481 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
      541 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
      601 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
      661 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
      721 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
      781 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
      841 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
      901 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
      961 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
     1021 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
     1081 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
     1141 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
     1201 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
     1261 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
     1321 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
     1381 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
     1441 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
     1501 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
     1561 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
     1621 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
     1681 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
     1741 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
     1801 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
     1861 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
     1921 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
     1981 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
     2041 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
     2101 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
     2161 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
     2221 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
     2281 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
     2341 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
     2401 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
     2461 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
     2521 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
     2581 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
     2641 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
     2701 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
     2761 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
     2821 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
     2881 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
     2941 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
     3001 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
     3061 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
     3121 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
     3181 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
     3241 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
     3301 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
     3361 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
     3421 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
     3481 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
     3541 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
     3601 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
     3661 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
     3721 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
     3781 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
     3841 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
     3901 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
     3961 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
     4021 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
     4081 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
     4141 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
     4201 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
     4261 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
     4321 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
     4381 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
     4441 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
     4501 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
     4561 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
     4621 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
     4681 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
     4741 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
     4801 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
     4861 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
     4921 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
     4981 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
     5041 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
     5101 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
     5161 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
     5221 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
     5281 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
     5341 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
     5401 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
     5461 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
     5521 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
     5581 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
     5641 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
     5701 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
     5761 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
     5821 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
     5881 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
     5941 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
     6001 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
     6061 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
     6121 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
     6181 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
     6241 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
     6301 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
     6361 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
     6421 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
     6481 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
     6541 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
     6601 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
     6661 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
     6721 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
     6781 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
     6841 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
     6901 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
     6961 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
     7021 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
     7081 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
     7141 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
     7201 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
     7261 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
     7321 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
     7381 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
     7441 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
     7501 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
     7561 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
     7621 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
     7681 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
     7741 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
     7801 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
     7861 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
     7921 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
     7981 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
     8041 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
     8101 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
     8161 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
     8221 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
     8281 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
     8341 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
     8401 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
     8461 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
     8521 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
     8581 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
     8641 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
     8701 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
     8761 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
     8821 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
     8881 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
     8941 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
     9001 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
     9061 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
     9121 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
     9181 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
     9241 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
     9301 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
     9361 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
     9421 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
     9481 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
     9541 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
     9601 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcaagag
     9661 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
     9721 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
     9781 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
     9841 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
     9901 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
     9961 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    10021 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    10081 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    10141 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    10201 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    10261 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    10321 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    10381 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    10441 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    10501 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    10561 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    10621 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    10681 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    10741 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    10801 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    10861 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    10921 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    10981 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    11041 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    11101 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    11161 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    11221 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    11281 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    11341 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    11401 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    11461 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    11521 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    11581 ttgt
//
LOCUS       DAZKCN010000078        11513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000078 DAZKCN010000000
VERSION     DAZKCN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.080"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            319..525
                     /locus_tag="ACG8K3_002007"
     CDS             319..525
                     /locus_tag="ACG8K3_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP9145861.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(727..1728)
                     /locus_tag="ACG8K3_002008"
     CDS             complement(727..1728)
                     /locus_tag="ACG8K3_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9145862.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1733..3646)
                     /locus_tag="ACG8K3_002009"
     CDS             complement(1733..3646)
                     /locus_tag="ACG8K3_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9145863.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3814..4320)
                     /locus_tag="ACG8K3_002010"
     CDS             complement(3814..4320)
                     /locus_tag="ACG8K3_002010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP9145864.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4480..4920
                     /locus_tag="ACG8K3_002011"
     CDS             4480..4920
                     /locus_tag="ACG8K3_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9145865.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4946..6061)
                     /locus_tag="ACG8K3_002012"
     CDS             complement(4946..6061)
                     /locus_tag="ACG8K3_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145866.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6106..6462)
                     /locus_tag="ACG8K3_002013"
     CDS             complement(6106..6462)
                     /locus_tag="ACG8K3_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145867.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6760..7734)
                     /gene="sgrA"
                     /locus_tag="ACG8K3_002014"
     CDS             complement(6760..7734)
                     /gene="sgrA"
                     /locus_tag="ACG8K3_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP9145868.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7930..8769)
                     /locus_tag="ACG8K3_002015"
     CDS             complement(7930..8769)
                     /locus_tag="ACG8K3_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP9145869.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8954..9841
                     /locus_tag="ACG8K3_002016"
     CDS             8954..9841
                     /locus_tag="ACG8K3_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9145870.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10435..11130)
                     /locus_tag="ACG8K3_002017"
     CDS             complement(10435..11130)
                     /locus_tag="ACG8K3_002017"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9145871.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11114..11512)
                     /locus_tag="ACG8K3_002018"
     CDS             complement(11114..11512)
                     /locus_tag="ACG8K3_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9145872.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3433 a         2295 c         1724 g         4061 t
ORIGIN      
        1 tcatttgtaa ttttatttca cagttgatat gattgttatg taaaataaaa aagtttagga
       61 tacatcgaat ttttggggaa attcttttgg gggtatccta aatcttttct attatattcc
      121 catattttct attattggtc tagacgtatg atggtaaact ttgtacctat ctgattgctg
      181 aatcattaat tgcttatcat tagccatctt tgtttcaagt tcgctagatg atcattttat
      241 tcacatccta acaaacttgt cacatttata gattcttaat ctatttatca tacaattata
      301 aaaaaggagg tgctgcttat gaaagctatc catatttctt tgcttttttt cgctagtgca
      361 ttgattttat ttgctattca tctttgttcc cagccttata tcaataacaa cggaatatta
      421 gtggaaccag cattcttttg cttaccactc ggctttttgt cccttcttgc ctccgcaggc
      481 ttcggatttg ttgctttttt taaacgatca aaacaacaaa tctgacttat aaaatttacg
      541 taaaagagat cgtgacaata atcgtgtgat attcccaaat ttcgaataaa aggtgcccct
      601 tgccatctct aactcctcag tcacaagtta caatattcat aaaccatgaa aagcttgttt
      661 attgaaattg ctccttgtga cttggagcta agtgacagat tacaacctct tttgcatcat
      721 aagactttac tccttgaaaa acattactaa cttttgatat gtttcttgcg cttgtttatt
      781 gctgaaatca aactgatatt catggttcaa aacagcattt ttctccgcat aatctacttt
      841 tgtgactttc acccccgctt tctcaaatac agaagttact tcttccattt gtttcgtaaa
      901 cgtatttgta tttccatccg ttaagaatgt gttttgcgtc caacgatccg catgtctgac
      961 caaggctaaa cttttgacct tctctgattg ttcaaagtcc ttattatcaa agtaagccca
     1021 tccacatttt tcataaagaa agctagagat tttatcactt acttgagtaa gtgcagaagg
     1081 atccaacaga ccagacaaag aaataaactt cgtaatctgc ttgttctcca aagtcggatc
     1141 aagatgatca gcttttttaa tttcttcatt ttgaagtgct aacaaatatt ctccactaat
     1201 ttgtgcacct gccgaatttc caccaatcga aatattcgac caatctgctt gatcttttat
     1261 ctctttttta gctgcttgaa tggcttcgtt caattggata actggtgtag gatgttttgc
     1321 ttccggtgat cgttcataat ctatatttac gatgatttgc tgcgtgtctg aacatagata
     1381 ttccaaataa tcacgacaat catttttatc ccctcctaca tatgcgcctc catgcatcca
     1441 gaaaagaatc ggtaattttt tcttttccac tttcggtgtg tagatatcca tattattatt
     1501 tttgtacttt gaaggatagg atatttcttt ttttatatcg atttgttcga ttttttgctg
     1561 gaaatcatct ggtcgggcat atacttgttc tttttcactt gtgtcaaatt gttttctaac
     1621 aagcaagcta atcggctttg gtgtgatttg attaatcaaa aatacagtga ctaaaataac
     1681 tacgatgata actggtactg caatcactat tcgcttgaac catttcattc atttaccctc
     1741 tctttcttcg ccaaatccat gtgataatcg tgctaacaga taaaaatcca cctgaataaa
     1801 tgagaagcga atgtttcttt tctccagttt tcggcaacat tctttttttt gcatctgtct
     1861 ttttttgtac tttcactgtc atctgaatcg gttgcgtatc atcacttgat tgatcctctc
     1921 gaggttcatt ctttccctta ttgtctgcat tctctgtcgg atcttgcttt tttggtggag
     1981 ctactgggat ttttttgtaa tagaaaataa tagttggtat cgtctcatta aaaatccctt
     2041 cagtcggtcc ttcaacattt gctaattcgt atccttcgaa tgtcttttgc tctgtcatat
     2101 aattttctcc ataagctccg taaagctgga tttcgtcctg gtctttgagt agacttttat
     2161 tgttttcatc cacatacttt acgtttatct gatcatggaa cactactttc aaaaagtccg
     2221 ttacatgttt cgtgtaggca gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt
     2281 ctactaataa aagatcttta taccctcctg ataactcata taatttttgc gcgttagaca
     2341 caggtactac atcatctgct gttccatgga taatcaattt aggcaatgaa tctgacattc
     2401 caatcgtagg tagagcagct tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt
     2461 tcaaaaggcc cataaataaa tcttcatgct cttgatcccc aatgatgtct aactgatttt
     2521 ttatggcatc cggtagtgcg ggggctactg tctggctgta cgtaaaacgt agttgttcat
     2581 caattgatgc aaatccgcaa ttttcaataa ttcctttgac attgacagga atatctttcg
     2641 ctaatacaga catcatcgta gctgctccca tcgaaccacc ataaagtaaa atcttttgag
     2701 atggccgctc tcgaacttct tgatttatcc agtaaaggac gtcatctgac tcatattgtc
     2761 cataggtcac ataatcgcct ccgctattac cagtcgctct ggaatcaaca aataataaat
     2821 tatacccggc cttataaaaa tcattgtatt cttctgtaac gatcccgtta ttccaattcc
     2881 ctcgaaatcc gccatggatc atgactgttt tatctgaacc ttgatcgaca taatatgcat
     2941 atacatccat cattttgcca tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat
     3001 ttggatagaa gcctgtgttt tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg
     3061 acatcaacac actgtttttt gcaaaagcga tatcgtacaa tagataagta gcaatcagat
     3121 gtttgataat ctctttatct acaccataat cattaacagc aagctcagtg actaataact
     3181 ctattaattt tgacttgatg tcttctgttg gtccattaag tatttctgtc atctgtttac
     3241 cgaagtaatt caaatattct ttaccattga aattttcacc tttaccgata tcatcatttt
     3301 ctgtcccatt ctctttgtca cttactttag cagaagagga ttcgtcgttt gacatttttg
     3361 tagctgttgc ttcttctgtt tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc
     3421 tagtaacaga tgtctcaacc gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt
     3481 tcgtttcatt tccagcatca gtagtttctg tggtttctac ctcccgagat tccgattcgg
     3541 aagaaatgct ctctccatat actgtccatt gcgggacgct tgtacttaca gcaaataaga
     3601 tgaccaacgc tccctttaat acttgttttg ttctcaagct attcactcct ttttatgttt
     3661 ctaggagaat tatatgtaaa cgcaaacatt ttttatacaa aaaataaatg gtttatccca
     3721 taaaaataga acaggattat tttctcattt tacgtaataa agcagaatgg ataaaccagt
     3781 atccattctg ctttttttaa acgaagaaac aacttattgc tccaaaaatg tcagaaatcg
     3841 tttatcccaa attttctcgt aaaaagcgat tgtttccttt gctttcataa aaggattatc
     3901 ataagttgtc gttgctcctc tacacatcat aagctggtat cctaatccat gagcaaactt
     3961 cacagtcgta tctatacaat actctgcttg tgccccacat aattcgatcg aatgacagtc
     4021 caattcagtt aataaaacac tcaaattagt cttataaaat gaattcgcat gggtttttct
     4081 cacaaaataa ctgtgctcag gaatagataa ttccgacaat agctcccatg gttttgtttg
     4141 tggcactaat tgagcatctt cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta
     4201 tgttttgatt cttttattca ctaatgctgt cactgtttct aattcgtgta tcggcgaaga
     4261 atcaaaacat accccttttt gtaaatcgat cactactatt gcgtcggctg tgtttgacat
     4321 acttgtgcct tctttctttt ttcttgattt aatagtattt cttatctagc tagttccatt
     4381 atacgtgaca acattttctt attttacacc tctaaatggc acttttcgac tatttcttca
     4441 tgttattatt tcataaatac ttagctattg gaggaatgta tgatgattaa attgtataca
     4501 ggttctggct gctcgtcttc aagacgcgca aaacgttggc ttatcgtcca tgatataccg
     4561 tttatcgaac agaacttaat tcgtcaacca ctaaccaaaa atgaatttat ccacttacta
     4621 aaattgacaa acaacggcgt gaccgaaatg ctctctactc attctattag ttataaaaat
     4681 cttgttcaca gaatagatga actcagtttg agtgaactat atattgagat tcgtacatcc
     4741 ccttctttat tgaaaaaacc gattatctac gaccaatctg caaaactttt gactggattt
     4801 agcgaagata aaatcaggat gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa
     4861 ctcttgcttt ctggtcatct tgtcgatttt aaagaaaata atctttttgc aatgtcttaa
     4921 ttttggcata aacaatctgc ttttcttaat taacgaaatc caatttttcc aatatggatc
     4981 tatttttctc attcctgatt tttatgattt cagctttcag acgcgcccaa tcattttttg
     5041 taggttgtcc attccagtaa aatatctgtg tattcgtttg atcatatgcc tttttgtcga
     5101 taggataatc gctaatgaca atatcaggta actgatcagc ggatgcacta tattttattg
     5161 ggcgttcagt catagataat atccttttct ttgcccataa ttcctgtgtt ttaccatact
     5221 ttgattgaat atagaatatg acaggttgtt cgtgttttat aaaaatatcc ctgactaaca
     5281 tacagtattg aaaaatcaac cgtctgttct tttcataata agtattaagc tctggaattc
     5341 tgcgagctac cccactaaaa aactgttgca tgattggata catcactgga aatatatgct
     5401 gaaaatctct gtcttctgct gattttccaa aagcatccac cttctttttt gtcccaaaaa
     5461 cccttgaata caaatggatc attgccaaat tgacatatag ataatttttt tctgaagttg
     5521 taaaacaaat cttgaagttt ttttcaaaat tttctacaat agacgattca atccagcccc
     5581 aactttcttg tactttatct ggagtatcca acaaatcttc ttccaaatag gtgttcatta
     5641 caccaaacat aaaataaagg taagcttgct ctgttggagt gatgtgattc atcgaccaga
     5701 agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg
     5761 gatgcacaaa tttgttcatc tcttctggga catacgtaat cgagtggcct tgtttcattc
     5821 gttgcaacga aattcttagt aggctgatcc atttctttct atctatacta cgcgtatatg
     5881 gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt ttttatactt gataatatat
     5941 cagaatgatt ttcgtcgaat gactgccaat acatcaagtg aaaaaagtat cggatacggt
     6001 attcttctcc ttctatcgga ttaccttttt tcaagctgca agataaatga tagtttttca
     6061 aaatagtatt aagttctctt aattttcttt gaatcgtacg caaacttata taattttttg
     6121 actaagattt tctaaggatt gataagtacc tttgtaaagt gaatccagta aaatataacc
     6181 aatcgttccc tctaaaagta tagaataaac tttactcagt aaaaactgag aagaaaactc
     6241 gacttttatc atcttgtttt ctgaaagaaa agataattta tattctggat acctttcgca
     6301 aaacgattct gcttgttgaa tcaccgaatt catcttagcc agggtgtagt ttagatctaa
     6361 agcgatatcc ttcaccacat ataatttctc gtctctttca taacaatagt tcaaaaattg
     6421 atatacttgc ctttcattga tatcaataaa ttcttcaaac aaagtacata ctccttttta
     6481 tctattattt agacttattc cgacagctac atgaataacg tacaaataga ttcgttactg
     6541 atttttcaga gatactacca tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc
     6601 catttgtgtt cctgttattt cctgtttctc aaatatgtta gtaaacaaaa atagatctgg
     6661 caaagctaca acttcgccag gcaatacagc actctcgaat gaaatattca ctcaaaaatc
     6721 tatcgtttta ttcaatttta gatctgttgc ttttattctt tatttcactc gatggaagag
     6781 agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac acacccatcc atgtaattag
     6841 cttgttcaag gttctactac cagtttctgg taaatcgctt tttctattat ctgtttcttt
     6901 cgctttttct tgatgatcaa ttccacgaga aacaaaatca ggctgtttta gcacactttt
     6961 taggttgctc cctgcatttc tcttttgttc cttagttggt atgataggta ccaaatcaag
     7021 ttcattattt acttttcctg tgctgctttc gctgctcgtg cttcctgtgc tactctcact
     7081 actcatgctt cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt
     7141 gcttcctgtg ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgttttc
     7201 tgtgctactt tcactgctcg tactctctgt actgctttca ccgctcgtgt tttctgtact
     7261 gctttcacta cttgtgctat ccgtactgct tggcggtcca acaactgtcc aactgcttgg
     7321 tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa acaatcaaat caactttatc
     7381 tttactcttg atctcatcaa tcgatagttc tgcagaaatc attattgtat actcaatacc
     7441 agcggtccat tgtacgtctt ttaaataaac ttccccaata actttttctg aacttccatt
     7501 accatcccaa ccatatgtta gaacagcatg tacatcacta gcattcacgc ttttgtttgg
     7561 tttgactgga atagtaatga cttgttgtgt catgtcataa acaatccgat cagttttcac
     7621 agtaaatgaa agattctcat ttgcccgttc atttgcagaa gtactgattc caatcattgc
     7681 tgcaattata ctcagcccaa gaataacaaa taatacggtc gctgttttct tcatttctat
     7741 cactctccat ataattacct tctatttgat aaaggaatta tataaaatga aaatgatgag
     7801 aacacgacag tcagttgtga aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt
     7861 tactttttcg tttaatcaat gacagactta aaaaagcaag accgatatca gccttgcttt
     7921 cttcatcttt tattgataaa cagcagtgta tttcttcatc cgctttgttt tatatgcata
     7981 gagtaacaaa ccaacaatca agactaacaa gaataatgta gttgatatag atgcttcagc
     8041 cccaaagttt cccccagtaa ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc
     8101 ttgttcttgc gttccgctga ctggtatacc caaaatagct ccttgtgtcc aattccaagt
     8161 aatatgagca ccaatagccg taaataaact tcctgtactg tagataagtg cgctgaaaaa
     8221 gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg
     8281 caaaagagca aataatgcag cagatccaat cattgctatt aacggaccaa atctaagcat
     8341 ctgatgctga aaaaagccac ggaataaaag ctcttctgat gcccccactg caataaataa
     8401 aataaaactc gttatcagcg gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc
     8461 cgtcaaatat aatggactag taataagcag aaacactcca attccaacaa cgagacctcc
     8521 cgctaaatac ccccagtctt ttcctcgaac agaaacacca taatcttcta ttctttttcc
     8581 ttcgacctta ctagcaagaa ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc
     8641 ggcaattgct ccaatccatt cactatttcc aataaacata tccgcaagtg cattattgac
     8701 aagaattcct aaaacaaaac caactaagac tattaaaaca gatccaatca accgaataat
     8761 cctgatcatc tttcatccct cctttatatt aagtttaaca acccttttct ttcttgtctc
     8821 gtgaaaacta tgtaaatgtt ctccatcata gagagaatag atttttacga actaagaata
     8881 tctacagcaa ccttacgaaa atgaaagact gtctccctaa ttcttctgtt agactgtttt
     8941 taataaggaa aggatgggaa attttttgaa aaaacaacta ctaattatgt gcctttcagc
     9001 atgtgcaatt ttactattct caggttgtag caagtccaat gaaacgattg caacgtttaa
     9061 aggtggaaca cttacagttc aggattttta tgataaccca aaagttaaat caaataatca
     9121 aacactatta aaaaaaatga ttgtcttcaa agcatttgat gatgtatacg gaaaagagat
     9181 ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc aaaaaactcg gtccaaacta
     9241 caaggaacaa ttaaaagcag ttggccaaac agaagagaca tataagaaac tctttaaaca
     9301 gatgctggct ttccaatacg gtttgaaagc caatgtaaaa ttaacagata aagatctaga
     9361 cactgcttgg aaagaattct atccagaagt tagtacacag atcattctat tttcgactaa
     9421 agaagaagca gataaagcaa aaaaagaagc aaatgaagga gaaaatttct ccaagcttgt
     9481 tcaagcttat ggaaaaaata aaacattaaa ggaaactgat ggaaaaatga attttgattc
     9541 aaccaatcca gagattccta ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga
     9601 agtttctgat atcattcctg taacagatcc aacgacttat cagcaaagct attatctagt
     9661 aaaaatggta aaaaaacagg ataaaggttc taataaagac aaatacaaat ctgagctaga
     9721 aaaaattgct accgaagcaa aaactcttga tacggaattc atggataaaa caatccgtaa
     9781 agtgatgaaa aaagacaatg taactattaa agatccttat gtaaaaaata tattcaaata
     9841 aacaaatttg acccaattgg ggatgtggca aaatttgaga agtgattttt ggaaattcct
     9901 tcttattttt gaggcaaacc acttttgcca caacttcatc ggggcctttt tttatttgta
     9961 ccaaaacaaa tcactggatt tgttacaatc atgaggatat aatttaaaaa agttttttat
    10021 ttctatcaca tgaatcaatg cgccattaag tagattcttt catgcttccc tattttttca
    10081 cttgccgtat cgcttcaaaa acaactatca taaactcaaa ctcgactgaa aagttatttt
    10141 aaaaaagact aaaaatatcc cttctggact tgttcttctt attctgttat actagagaaa
    10201 cacgaaagat atttttatgc gacacttttc atttgttttt atgaaattta ttcaaaactg
    10261 gcgtaacttc agagttacaa ggatgtatta gaggttaggg aatacatcgt ttaaatgaga
    10321 aataagtaat agttagtaat agtcacccaa actttaaatt aatgaatatt gaatcgtcag
    10381 atagcctcct cgagttttcg agaaggctat cgtttatttt atggagaaat tgattcagaa
    10441 gtaatcagcg acctttttta gttcaccaat cgtttctgct tggaaagttt gcacccaatc
    10501 ttcttggaag cccctcactg ctttatcaat cgagggcatc tctaatactt gatctagtag
    10561 ctctttacct aacgttatcg catgagctcc agatcgaatg gctgtagtga cttgttcaca
    10621 atttttgaaa ctagctgcta atatttttgt tggtctttgc gtacgttcga tctcagtccg
    10681 caacgaatca atcactttca tagcatcaat gccttgattt tgcatcctgt tataataagg
    10741 tgcaatataa tctgctccat tattaatagc taaataccct tgtaattctg tgtaaatagc
    10801 tgtaccggta atcaaataac cttctttttt caactttttg atcgcttgaa ggcctgcttg
    10861 attcaccggc accttgataa acgtatcttt tcccagttct tctatgataa ctcgagcatc
    10921 ctctatcatt tcttcttccg ttcttcctat tgtctgaata tggataggaa tctgtccgat
    10981 tatttttttg atttctttca tatgcgagaa gaagtttgac gctccttctt ttttcagaat
    11041 tgtaggattt gttgttacgc ctgttaaatt caaaaactgt tgacaagaat aaatactttt
    11101 tgtatttgct gtatcaagca tgatttccat atagctttcc cccattctga tcctcttgat
    11161 cgattttaat gattgtcccc tttcttaaat gcggcatttc cttatcttct acatacattg
    11221 ttcctggaag ttccggctca tgagatccat caaattttat cgtgatatgt cctaattcat
    11281 tcaaattagt ttggacaaca tctcctactg ctgtgattac atacatttgt ttatcaaaac
    11341 agagcttcat atcacaagtt atttcttttc ttacctgatt caattcaatc ttataacaat
    11401 aatctgttaa tgtttccggt gcttcatctc caaataaaat cattattttt tctttcctga
    11461 atacttctgc ttctatacca acatctgtta caaccgtttc aaagatcgtc atc
//
LOCUS       DAZKCN010000079        11461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000079 DAZKCN010000000
VERSION     DAZKCN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.081"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..735)
                     /locus_tag="ACG8K3_002019"
                     /pseudo
     CDS             complement(205..735)
                     /locus_tag="ACG8K3_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            807..2099
                     /locus_tag="ACG8K3_002020"
     CDS             807..2099
                     /locus_tag="ACG8K3_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFP9145873.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(2311..3153)
                     /locus_tag="ACG8K3_002021"
     CDS             complement(2311..3153)
                     /locus_tag="ACG8K3_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9145874.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(3190..4656)
                     /locus_tag="ACG8K3_002022"
     CDS             complement(3190..4656)
                     /locus_tag="ACG8K3_002022"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9145875.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4712..6556)
                     /locus_tag="ACG8K3_002023"
     CDS             complement(4712..6556)
                     /locus_tag="ACG8K3_002023"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP9145876.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            7158..8228
                     /locus_tag="ACG8K3_002024"
     CDS             7158..8228
                     /locus_tag="ACG8K3_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9145877.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            complement(8194..8280)
                     /locus_tag="ACG8K3_002025"
                     /pseudo
     CDS             complement(8194..8280)
                     /locus_tag="ACG8K3_002025"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(8324..8785)
                     /locus_tag="ACG8K3_002026"
     CDS             complement(8324..8785)
                     /locus_tag="ACG8K3_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFP9145878.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(9045..9593)
                     /locus_tag="ACG8K3_002027"
     CDS             complement(9045..9593)
                     /locus_tag="ACG8K3_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145879.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(9594..10451)
                     /locus_tag="ACG8K3_002028"
     CDS             complement(9594..10451)
                     /locus_tag="ACG8K3_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9145880.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(10737..11024)
                     /locus_tag="ACG8K3_002029"
     CDS             complement(10737..11024)
                     /locus_tag="ACG8K3_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFP9145881.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(11014..11343)
                     /locus_tag="ACG8K3_002030"
     CDS             complement(11014..11343)
                     /locus_tag="ACG8K3_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9145882.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         3497 a         2297 c         1805 g         3862 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taacatttcc atttcattta
      121 cctctttttc gtttaattat actaaagttc tatccaagcg acaagctgtg tataataaaa
      181 atatacccgt ggtgctagtt aattttaata ccctaaagaa tatctcaaag tcccttcaaa
      241 atgttgctgt gctcggctta aaagaatact gtacgataat aatatgcttt caaacttact
      301 ttctagccct tgaatagaac gtgatttgaa attttgaaaa cctagtcttt cgagagaaga
      361 aaaaacagtt tctactgctt ttctttgtct agtaagtaat cgatggtcta tcttccctag
      421 tttatccatg ttttttcgtg acggaactaa gactgtaatg cccttaaggg caagcttttc
      481 atggatttta tggctgacat agcctttatc tccaaggata tttggaaggt ttgcttcttc
      541 cgataaagtc tctaaaactt tcacatcatg aacactagga tccgtgattg aataagtaac
      601 tggaaagcct gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt
      661 cgttgagtta tagcccctac agctctatac tttttgatgt tggtggatga caccagcatc
      721 ttttttattt tccaaaacat atagaaaaga caccttgaaa tcctttagaa ttaatttggc
      781 gaaccaaccc aaaaggagga atcaagatgt cttacactca ccttatcaaa gaaacattag
      841 atattctaga cttatcagtt acttttaacg aaaattgtct cacaaaagaa aaatacaaag
      901 ggcaaatctg tcatatttat cgtggaaact tgatttatac agcccaagaa tgcatccact
      961 gtaaacacca gattgcttca gatattgtgc gttggggtac aacaaccgtc cggttgttaa
     1021 tgaacgatgt ttctgaatgc cgtacctacc tcgaattgaa aaagcaacgc ttcaaatgca
     1081 aagcttgtca gcggaccttt gtcgcagata cttctgttgc gaaaaaacac tgtttcatca
     1141 gtgagaaagt ccgctggtct gtcgtgactc gtctgaaaaa aaatacctca atgacagaga
     1201 tcgccgctca gaaaaatcta tcggtctctt ctgtttaccg tattatgaag cgattttatc
     1261 gtccgttgaa tccttttcgg acaccgttac ctaaggtact ttgttttgat gaattcaaat
     1321 cagttcgggg tgtttcaggt gctatgagtt tcatcatgat ggatggacaa acacagcgct
     1381 tgttggacat tgtcgagaac cgacaactgc cttttttaaa acgttatttt agtcattttt
     1441 cacgagaaat tcgtgaagcg gtcgaatgga tcgtgattga tatgtacgct ccttatgtat
     1501 cgctcgttaa aaagctgttt ccaaaagcac agctgattat tgaccggttt cacatcgtcc
     1561 aacatattgg tcgtaccttt cgcaatcatc gaatcaaaga aacgaaccaa ctattaaaaa
     1621 gcaaagaaca aaaacactat caattaggta agcaattaaa aagatactgg aaacttcttc
     1681 aaaaagatga acgcaagctt gattatactc gacgcctttg gcgacctggc ttcaaagctc
     1741 atttaacaga gaccgatatt gtggatcgct tactgaaagg gagccctgct ttgcgtgtag
     1801 gctatcagtt atatcaagac tttctttacg ctgtaaagga acgagactac gtatcgtttg
     1861 aagaactact taccaataat atcatgcttc ccgaaggcta tcagacgacc ttgagaacgt
     1921 tccagaaatt tctgccacaa atcaaaaatg ctttacaaca aagttattca aatggcccac
     1981 ttgaatgtct gaacaaccat atcaaagtgt taaaacgaaa tgcatatggc tttcgtagct
     2041 tttataattt caaattgcgc atcatgattc gtcatggcaa cgcattgatt ttcaactaaa
     2101 aaagatcaag aggaactgat ccacttgatc tccaattatt ctaagatttc tctttaccaa
     2161 catcatttga caaagagccg agatttttta ttatcttacg ttaatccttt ttaaatgtct
     2221 cttatagata acaaacagct ggaaatcacc ttgtttttac tataatccat ctctaaagat
     2281 tttaaaagca tcaatagtat tactgagcgc tcacccatta gcatgtttca tcaaccgatt
     2341 gatgtggaca cctaggtata caacttcatc gttcggaatt gttttatgat aaactttttc
     2401 aatgtagtct ttgatcttag ctgcgctttc catggcagac gggtataaga tccacatttg
     2461 attgtagaat tcttcctcac catcatccaa caaagcatca gaataatatc tttctacaaa
     2521 gaaacgtaca tgtgtaatga agcgggcata atggatcgaa ttagtatcga tatctttctg
     2581 caacgaatac cggaccaaat tgacgatccc gccgatcatt tttgcatact tgaatccagc
     2641 gccgctatca ggatcttccg attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc
     2701 ctctttagga agatcaattc catattgctc gttcaaaagg gtgagtgcgt acttcccaat
     2761 ttgaaattct ttgggatagt agatttttat ttcccagtac aaacggtttg caatatttac
     2821 ccctttttgt gcgcgttcaa ctgcaaaatg caggtggtct gttaaggtta aatagactcc
     2881 aggattcagt tttttatcta atcctttttc tgcttcaaaa acgatagtac gagtgatttc
     2941 aaaaaattca ataggtattt cttcaactaa ctctaatagt tgtgaagatt tcttttcttc
     3001 aaaaggcaga aatattttat ctactttatc tcctgatatt tcatccccag ctttctgtcc
     3061 aaagcctatc cctttaccta atgcaatcat ttctttgcca ttttcttcaa caagtaccac
     3121 acttgaattc aatacttttt ttattgaaat cactcattat ccccctatcg ctgcttaaaa
     3181 agatcgagac tactcgaata aggatgcacc gtttgtttcg atcacttctt tgtaccagtt
     3241 gaacgaatcc tttttcaagc ggttatatgt cccatttcct tcatcgtctg catccacata
     3301 aataaatcca tatcgtttag acatttgaga agttcctgca ctaactaaat caattggtcc
     3361 ccagctagta taagctatca aatctacacc ttcatcgatt gcttctttca tttgttggaa
     3421 gtgtgcttta aagtagtcga tgcggtacgg atcatggatt ttaccatcta ctacctgatc
     3481 gactgctccc ataccatttt ccacaatcat caacggtact tggtaacgat cataaagttc
     3541 gatcaacgaa attttcaaac cttttggatc gatttgccag ccccaatctg ttgaaggcaa
     3601 atgaggattc ttaaccccta aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc
     3661 tgattctgcc atggacatgt aatagctgaa agaaacaaaa tctacggtat gtttctttaa
     3721 aatttctcga tcttcatctt ccattttaat ctgaattccc cgattttcca aatccttttc
     3781 gattagagca ggatattctc ccttaacttg aacatctgca taaaaattat tttctaagtt
     3841 ctttttcaag gtcagctcaa cgtcttctgg tgcgcaagtt aatggataag tcatcaattt
     3901 cgttaacata caaccaactt ggcttcctgg aattttttca tgagcatctt ttgtcactaa
     3961 cgcactcgca acaaattgat gatgcaatgc ttgatagata tcttgagctt cttgaccttc
     4021 tgcgcttttc tcttggataa ttccagctgt tgtaaatgga tgacggtgga tgctgtctat
     4081 ctcattaaat gtcagccaat attttacgta tttgccgaag cgatcaaaac aaacgttagc
     4141 gaagcgaaca aaatcatcaa ttactcgacg ctccacccag ccattatatt taactgctaa
     4201 agctaatggc atttcataat gagatagtgt cacaatcggc tcaattccta aacgttgcat
     4261 ttctttaaag acgttttcat aaaatgcaac ccctttttca ttcggttcca gctcttctcc
     4321 tgtagggaac aagcgagtcc aagcaataga gaggcgcaag gttttaaagc ccatttcggc
     4381 aaataaagct aaatcttctt tatagtgatg ataaaaatca acgccccttc tttttgggta
     4441 ccatttatca gatttgtctt cactagcttc ttttatagac tgcagggtga catgcatatg
     4501 agcagcaaaa tctttgttgt ctaaatttgg tttataagta gccatatccg ctactgaaag
     4561 acctttaccg tctacattcc aagcaccttc tacttgattt gctgctatcg cgccgcccca
     4621 taaaaatcct tttggaaaac ttgttgtgtt tgtcattatt ctattctcct ctattccttc
     4681 attcaatata ttttttttca ctatcttgtg tttaagctat cggactgata gctaagagat
     4741 cactatcatt ggttaccaat ttttctaggt taaatgaacg gatagtgtaa ttatccttat
     4801 tggttaccac acagataact gtggggtcga atcctgcttc tttgattaaa tcaatatcaa
     4861 atgcaatcag cttttgtcct ttggctactt tttcaccaga acgaacaaat gcctcaaacc
     4921 cttttccttc taactgaaca gtatcaatac caatatgaaa aagaagttct gcaccattag
     4981 ccgtttttaa accaatagca tgcttcgttt caaataccat tgtgatttct ccatcttcag
     5041 gagctactaa tatcccttct tcaggaatga ttgctactcc atcacctacc ataccactag
     5101 aaaaaacagt gtcttttact tctgatagtg agattacttt tccattcacg ggacttgaaa
     5161 gaacagcatc tgaaacaacg gccgtttcca tcttagtttc aacttcattg acaacttctt
     5221 tatctttata aagaaccaac gctgcagcaa aagcaaccac aaatgaaatc cccgatactg
     5281 caaatgccca aaccagattc attgggtcag ctttgtcagc gaacatcgtg atacttgcaa
     5341 tcccaggacc cactaaagca aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga
     5401 tcaggctaga aaacataacg gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa
     5461 gagctggttc agtaattccg aataatgcag atatacccgc agaaacagcc gtagatttca
     5521 attttttgtc ttttgttttg atactgacag caaagcatgc gccagcttct gaaatattgt
     5581 gtgccaatga agccgggaga taaagaagtt ctgcacccaa ttcactcatg gatgaaacag
     5641 cgtaagggat cattgcttta tgcatacccg ttacaaccat taatggcagt acagctgcta
     5701 agactccggt tgcaacccat ccaaattgat tgtacaaaga cacaatgata gaagagaaag
     5761 ctgttcctac attataacct aatggtccta gaagcaacaa agcaatagga actgtaatca
     5821 ataagctcat cataggtaca aagaaaatac gaattggttt tggtgaaact tttgtaaacc
     5881 atttttcaag ttgagcataa aataaaacag ttaagatggc tgggaatact tgagatgcat
     5941 acgctatatt atccaatcca aaagataaga atgtagcacc ttccgccaac atagctgtca
     6001 tttcaggaag gatcaatgca ccgacagcag atactgcaac caattgattg actttcaatt
     6061 tattagccgt agtcattgcc accagaatag gcaagaaata aagcggtgcc ccgccgacca
     6121 aatttaaaat ctgataggtt tgagattggt cactaatcag ccctaagata gaagctagta
     6181 gcaacaagga cttcaatact cctccacctg caatcgcagg aattaaaggt tggaaaatag
     6241 aaataataaa atctaataga actgaaccta atttttgttt ttgttttggt gcttcttcag
     6301 tatttgaaac attgtcacct aataacgctt tcacttcatc atacacttca acaacatcgt
     6361 ttccaatgat tacttggcat tgcacatttt ggcgaacgcc cataacgcca tccacttttt
     6421 ctaactggct catatttacc ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc
     6481 aatgctccaa atgggatatg ttttcttccc caccgatgta ctgcaatact tcttctgctg
     6541 tttgtttgta attcatggtg tttccctctt ttcttttttg agtgatgaac ttttgagtcg
     6601 aagtggccat cctcaacaca aaaaacaggc agaaactaaa taaattgaca acaaggcaaa
     6661 cgtgtattga aacgcataca ttgttattca acttatttag gtcctgcctg cattaccagt
     6721 cacatgcctg tttaagttat attcaattta acatattaga atgtaaacgt aaataataaa
     6781 atgatttttt aatcattttt tcattttatt cgttcaatat agttttcgtg ttctaaaagt
     6841 tttgtctttt ttctaagaat atcttccaac atatataact cttattctct tctatcttct
     6901 agcagatgtt ctgtcttttt aagacctagt ataaaaagaa tgggttcatt ttttctttct
     6961 tgcaggatga ggttttttat ataaacttta tttgatataa agaaaaggca ttcataattg
     7021 tgctatacta ctaaaaaatg ataatacagt gaattagatt agcgctttat taaattaaga
     7081 ggacagcata tcgatattgc ttcctttttt ttgctattta tttaagggat agaagtttag
     7141 aaaggactgt ttataagatg aaaaatgata tagtgactta tgcaaacaca aaatgtgaag
     7201 aaattggcat cagtaattta gaagagtgct tttttgacac aaatgacctg tcgcaatttt
     7261 taagaacggg aagaacaaat gccagtaaac ttgccaatga cttagttgca aagaacgttc
     7321 tatttaaaat caatactcgt ccagtgttat ttgccatcaa agagcgcttt gaagagcact
     7381 ttttgattcc ggtaaaagat gtatacgaaa gtatatctga attaaaaaca gacttggagc
     7441 ccaattctac agattccatt ctacaaaaga tgattgggta tgatatgagc ttatccgatg
     7501 cggtggaaca aatgaaaact gctgtcttct atcctggaag agggcttcct atgatgatca
     7561 ctggagatac tggagttgga aaaagttttt ttgttcatac tgcttatcag tttatgaaaa
     7621 aagtgaacgt tattcatgag aatgctcctt ttaaagtact taattgtgcg caataccata
     7681 ataatcctga attattgtcg gggatactat ttggctatgt taaaggtgct tttaccggcg
     7741 cagatgaaga tcgaaatgga ttgttgcaag atgcagacgg tggagtactg tttttagacg
     7801 aagtacacag attgcctcct gaagggcaag agaaactatt tgtttatatg gatggcggaa
     7861 cttattctag agtcggtgaa actatccatc acgcctccaa tgtacgttta gtttttgcga
     7921 ctacagaagg caaagaggat tttctggaaa cctttatgcg aagaatccct atacatatcc
     7981 atatcccaaa tattgaggaa cgaggtattt tagaaaaaaa caaattatcg aaaatactct
     8041 ctttagcgaa agcaaaaact ttagcaaggc tattgatgtt tctcctaaag tcactagtgt
     8101 tgcataaata tagtcagaat attctgaatt attattttct tgaaaaaagt aaaaaaaatc
     8161 gtatcccaaa ctttcatcta catttccaga aagatcttca tgttgataat caattcctga
     8221 atcaatgacc gctaccttca catccttaga acccttggtt tcttcccaag cggatggcat
     8281 gctaatctct cttaaattag cttgtttact ccaattagga tcgttatcga tttcctaaag
     8341 aatcagttgt atttggctcc tcaacatcat tcaacggctc tagttcaacc acatagtttg
     8401 gctctgcata attgaccata tcagaagcat taaatgttct tactgcctct tccacactaa
     8461 tttcttcgtt caatttcact aataatactt gatctcgctt attataatcg ctttcatttg
     8521 tcctaagagt caaatctttt tccaccataa cttttacacc caataaattt tgcggtttat
     8581 ttttctctgt tgaaattctt tgtgtagcct ttaaactgac aattagctca ttgggtacat
     8641 actctatttg agagtcatct aattggctca taatcctttc tttttggtta gtatctgcag
     8701 ttacttgttt agtatgagcc atgaacccaa acatactcac cccaacaacc aatcctaaat
     8761 atattttctt tttaaacttt ttcatctaca tctcctccta atagttttat tttagagtta
     8821 gctctattac ttatttacta actcaacaac agattatcat acacttatta atgaaatcac
     8881 aatatctcat taaaacacac cctaaaatca taaccgttcc tatgctcatt ttttggattt
     8941 tttaatcacg taaccagaaa aataaatccc ctctatttca cgcaaatcaa agaagaacta
     9001 agtaactacc gtttaaaggt agttacttag ttcttctttg attattatct aactttatta
     9061 ttcattcccc accctttgcg cagtgagtct tcggtatctt gttttatctg actggtagtt
     9121 cttattattt ctccacgatc atctaattct ggaacaattg gatatttttc tctgaacaag
     9181 tctagttctt tcaccacgtc attaaacaaa tcttgtttat cttcttgcgt aagattctgt
     9241 ttaatatact catctaaagc ttttctaagc aattcatctc ttgtcatatt agtctcatac
     9301 ttttctacta aaactctgga agtcgcattt aacaaagtcg acagttttaa aggatgcttg
     9361 gtggaataaa ccttatcaaa ctttctgatt ggtgcgccaa ctttttttgt tcttcctcta
     9421 ctctgtcttt caacattgga ggaagttttt atttcttgaa cttggtgcac ttctaattgc
     9481 ttattttcag atggtgtaat tgcttgctgc tttttctttg cttctgcttc tttttttatt
     9541 ttggcgagtc ctttgttgtt cccaaaaata tcaagtcctt tgatgtcagc caattactcc
     9601 acctccgcat accaattctc gtgttcttcc aattctttca aaatactgat aaataattcg
     9661 tggcttttgc gatcccattg ttttaagaga cctttctttg tcttctccat cgtaatacct
     9721 gatccgtcgt atcgttttaa gcgcgcattt ttgagtacaa catttttaat caaatgttcc
     9781 ccataaatct cttgggcttg tttatacatt tcctgatcta ctgcgtcacc gctttccaac
     9841 atgaaaggaa taacaccagc aacatataaa tttgtatcaa attctttcac aaaaaagtcc
     9901 atatactcaa gatattgcat cgcatttctc atcgatttta cttgggtctg taagacgacg
     9961 attgcccaat ccgcaatgta catcgcggat ttactatata cagaaataga aggtgggaca
    10021 tctaaataga tgacgtcaaa ctcatcaaat aatggttcta gcttttcttt catatatttg
    10081 aattgggata atgaatcatt atattggtct ttgatccaat catcatacat ttcaaaggac
    10141 atgtctgctg gaagtatata gaggttattg attactttat agacagcctc tttaggtgga
    10201 agattgttta tcaacacgtc agtcacagta ttttcgtaaa caatcggttc accttctccg
    10261 cctccttctt cgttagtgaa gttcatatgc gttaagttca tcacatcaga agtatttcct
    10321 tgcatatcca agtcaattat caaggttctt tttccttcat taactgctga ttcaaaccct
    10381 aacatttgcg cgcacgtact tttacctacg ccacctttaa aattaccgat tacatatttt
    10441 ttcgctacca cgttcctacc tcttttcttt tagcaatatt catttgatac tattataaca
    10501 tatttatttt tagttactac caaaaaaaag atatatatgt actaccttat aattaataag
    10561 taaaaggtag tacataaata aggaaaagct gaaatatatt gatttatagc tttcctcaaa
    10621 gtaatgacgt tatttaattt atcgagaagc agagaatact cgcgacttta gtcatgtaga
    10681 tgaatcagat cttcgataaa aaagaagccg tccttcttaa gaacgacaac aaatcactag
    10741 attaattttt tcaactgttc ataaaagttt tcatgcgttc cacataacaa tactggaata
    10801 accttgtttt ctataatctc gtaagctact ctatatgttg tgcccgcata tttgaaccca
    10861 ctagaccaaa tacctgataa atccccagtt ttttgttcac ccttatatgg atcaatcgca
    10921 atttcatcat aaattaaatt caaaaatttt tctttcaatg tcttctcttt tagctttttt
    10981 aaatacttaa tgacatgttt tgaaggaacg atttcataag ccaatttctg ttgccaactc
    11041 ctctttagtc attactttag aattagtaag tgtatcttca gcaatatctc taaaggaaga
    11101 atgcattttg ttttttctat tcttaaattc tacaagaatc tcatctttat cgtagccttc
    11161 tgcaattata tcagacaaaa tatcttcgct aaaatcataa aattcttttt gtggttcaac
    11221 aaattcataa agaataccat tctctaccaa ctttgcttca acttcgactc cattaggaac
    11281 attaaaatct ttaggtactg tcaacatgat tgaattgcct tgttttcttg tttttgtaat
    11341 catttaaatc cctcatttct aacagtatgt aattacttag taaatacatt ataccacgag
    11401 ataatgtatt tacaagtaat tactgtgact cgtaatgcgt ccttgctgaa taaatttcaa
    11461 c
//
LOCUS       DAZKCN010000080        11181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000080 DAZKCN010000000
VERSION     DAZKCN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.082"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..304
                     /locus_tag="ACG8K3_002031"
     CDS             5..304
                     /locus_tag="ACG8K3_002031"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9145883.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="ACG8K3_002032"
     CDS             377..1738
                     /locus_tag="ACG8K3_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9145884.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="ACG8K3_002033"
     CDS             1760..2020
                     /locus_tag="ACG8K3_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145885.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="ACG8K3_002034"
     CDS             2034..2786
                     /locus_tag="ACG8K3_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP9145886.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="ACG8K3_002035"
     CDS             complement(2880..3485)
                     /locus_tag="ACG8K3_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9145887.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="ACG8K3_002036"
     CDS             complement(3538..4512)
                     /locus_tag="ACG8K3_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP9145888.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5609)
                     /locus_tag="ACG8K3_002037"
     CDS             complement(4890..5609)
                     /locus_tag="ACG8K3_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9145889.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ACG8K3_002038"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ACG8K3_002038"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP9145890.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="ACG8K3_002039"
     CDS             7160..8557
                     /locus_tag="ACG8K3_002039"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9145891.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="ACG8K3_002040"
     CDS             8638..9078
                     /locus_tag="ACG8K3_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9145892.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="ACG8K3_002041"
     CDS             9226..9552
                     /locus_tag="ACG8K3_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9145893.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="ACG8K3_002042"
     CDS             9553..10302
                     /locus_tag="ACG8K3_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP9145894.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10382..10837)
                     /locus_tag="ACG8K3_002043"
     CDS             complement(10382..10837)
                     /locus_tag="ACG8K3_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9145895.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3551 a         1958 c         2295 g         3377 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaaatttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agactgtgac acgcttttgt cacagtcttt
    10381 tttatatata ataatttaaa aattcatcat ctaaatccgt agaattttct agattgctta
    10441 cccatgtaaa atcgttatag acagatttca gttgacttct gcgccattgt acagggataa
    10501 aaagacgcat gctatcttca ttgaatccga ctaataattt ctttccgtca gtaatgatgg
    10561 gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac aagctctgag gtcgtacatt
    10621 ggtcgatttg gtctttcaaa tttttataat caatcgagcg tttagctaat agatcgtcaa
    10681 atccgttttc tgttaatgac aataaataaa atatctcttt ccttgttaaa ggctgtttag
    10741 gtgtgaaaat tcgttctctg taaggcattt gatggtcttt cagccagttt gccatcagat
    10801 ggtaactatg tgtgcgagaa gaatagatcg taagcataaa aaaactcctt aaatagtcca
    10861 ttaatttgtt agatctgttg agataaataa taggtaagga gctgtgcata agcattgatc
    10921 aaaacaaccc aaacatcatt agaaataact ttctaaagtc ttaaacttgc gacgagaaac
    10981 aactagacta atagaaaaaa tgtcttagcc atttggcttc ctgtttgttt ttttgcttat
    11041 gctgcagctc ctcgggaaag ggttctaaca atttgaagaa ggttgatgat tgatctagtt
    11101 taaattgtgt gaaatttgaa atctggtgtt tttactagat gaatcaccta acaactatga
    11161 ttataactta tagaacttct g
//
LOCUS       DAZKCN010000081        11145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000081 DAZKCN010000000
VERSION     DAZKCN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.083"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..221
                     /locus_tag="ACG8K3_002044"
                     /pseudo
     CDS             91..221
                     /locus_tag="ACG8K3_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031600262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(286..1062)
                     /locus_tag="ACG8K3_002045"
     CDS             complement(286..1062)
                     /locus_tag="ACG8K3_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9145896.1"
                     /translation="MIGFIYFIVIVLANTVGAVSGMGGGVLIKPIFDFIGAHSVAAIS
                     FYSTVAVFTMSLVSTARQLASGRKIHWQIVLWVSGGAVLGGILGNVAFDHLLLLFQNE
                     DEVQMIQIFLTVVTLLFAFFYTKYDWPGFQLKNNFWYCFCGLVLGFLASLLGIGGGPI
                     NVSLLMLMFALPIKEATVYSICTIFFSQLAKIVTIAGTTGFGLYDLKILWFVIPAAIV
                     GGLLGAKVSTVLSPQKVTLVFQTVIFLVLIINLYNGYQLL"
     gene            complement(1059..2171)
                     /locus_tag="ACG8K3_002046"
     CDS             complement(1059..2171)
                     /locus_tag="ACG8K3_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFP9145897.1"
                     /translation="MKHISVLIKPASSLCNLRCSYCFYANVSSLREVRSFGKMKEEVT
                     EKMIENIYADLEDGDQLTLAFQGGEPTMAGLNYFRTVIRLVAQQQKQVQVHYAIQTNG
                     TMINEKWCAFLKEHDFLVGLSIDGHPLYHDLHRVDPKGRGTFQRVLQTKQLFDYYEID
                     YNVLCVLTNPLAKEAKKVFRFLKEQQIDFVQFIPCLDDLDAKKRNSYALTPKRFAGFY
                     HQLLQLWLQELAEGHYISIKLFDDLLNLLIKRQVTACGILGNCQVQYVIEADGSVYPC
                     DFYVLDEYRMGYIQEQTLRQLFEQDIAKTFLCGRPEMPKKCLSCPFQKMCRGGCKRMK
                     DAIYVDNTGYCGYQQLLQEFVPQINRILGLLQGVSV"
     gene            complement(2252..3022)
                     /locus_tag="ACG8K3_002047"
     CDS             complement(2252..3022)
                     /locus_tag="ACG8K3_002047"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9145898.1"
                     /translation="MKSQQFFFKYVNIDASNLKGELIIKPKYLEIKQNLKQQIVTGQF
                     HNGDRFYSESEIIKKYNVSSITAIRALKELVAEGYIVRYQGIGTFVSRARKEKIVKFS
                     DIEIFSFDNDKTHVLSIKRENKKQILDTLKLSTDSYYYQIERIREANEIPYIYHKSYL
                     PEQYINSNYPEINYYNSIYKRLKSDYNIDLNEENFRETNEILFPAPNSVCKQLKINES
                     EPVVFQTKLSCLSENNKPIEYVESYKKWNFYKIQLLSN"
     gene            3076..4854
                     /locus_tag="ACG8K3_002048"
     CDS             3076..4854
                     /locus_tag="ACG8K3_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFP9145899.1"
                     /translation="MTTFEIKEEFLLKGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNEIAYLEHVADYYDVLMKRIVPHQLNNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMIKRGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     NSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPVQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQELQATQYQTEIGEDIQVSMTEEINQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDIGDCFIDLRKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
     gene            4858..5337
                     /locus_tag="ACG8K3_002049"
     CDS             4858..5337
                     /locus_tag="ACG8K3_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9145900.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSILPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEI
                     NVGNMSQTSETRSITKSINVVDQDVETFNQLNEKGVHLIAQMVPSDKADDFMSLLNK"
     gene            5360..5767
                     /locus_tag="ACG8K3_002050"
     CDS             5360..5767
                     /locus_tag="ACG8K3_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9145901.1"
                     /translation="MAGHLVLISHGRFCEELKRSVDMIMGVQKDIYTVGLLPEESAKD
                     FRVNFEKTINGLKKVTVLADLMGGTPCNVVSQLILEGKEIELYAGMNMPMVIGFVNNQ
                     FLGEDSSLVNKGRENIIHVNEYLEVNPIDLEDE"
     gene            6018..6335
                     /locus_tag="ACG8K3_002051"
     CDS             6018..6335
                     /locus_tag="ACG8K3_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145902.1"
                     /translation="MVFEDQYLQIKNSENIEACIEISNSQESNIFVSPENGPVKPNFN
                     YLTYDRFSQNTVFDGYKLEQSSPAIHSGKKVIDKNGYNLGTDFFGIKLDGILDIGAVK
                     SSK"
     gene            6357..7169
                     /locus_tag="ACG8K3_002052"
     CDS             6357..7169
                     /locus_tag="ACG8K3_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9145903.1"
                     /translation="MNDISFFQIFSLICLAILGGIDPYLSGLQLSKPAISGFLAGIIM
                     GDINTGLLVGATLQLMVLGVGTFGGSSIPDFGTGAIIGTALGVVSGKGIEFAIGISVP
                     VGLLLVQLDILARFCGVYFLHRVDKHIEEENFHKISLEAWLSLVPIALSRAIPVGLCL
                     IFGNEVVNTVLNYAPDWLMGGLKLAGAILPVVGIAILLHYLPIRNFFGFLIIGYILAA
                     YLNIPMMGISFAGLAAAIIHYKKLQDKRLLESKLDTINHIEVVNGEVDDDEI"
     gene            7156..7971
                     /locus_tag="ACG8K3_002053"
     CDS             7156..7971
                     /locus_tag="ACG8K3_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9145904.1"
                     /translation="MMKSNVSKLSSKDLRKVSLRYMFGAQLGWNYERMMNVAYVRAVY
                     PALKKLYKDEKELKSVLQSEMQFYNTSPFLSAFIIGIDLALQVEDGSKSKDAVAAIKT
                     SMMGPFAAVGDSLFGAVIPTIFGSLAAYMGLEGNPLGVLLWLLVSLIILFLRYFELPI
                     AFREGKKLVSSVGGLLKDLTESATLLGVVVIGGLVPSVVKVGVPFKLVIGDKKLSFQT
                     EMLDPIMPAIVPVVLVLLAYWLLSKKSLNSTKVIWIFLVLSILMYSLNILAVL"
     gene            8290..9747
                     /locus_tag="ACG8K3_002054"
     CDS             8290..9747
                     /locus_tag="ACG8K3_002054"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HFP9145905.1"
                     /translation="MKKNQPNVVLIVVDQMRADALSLNSQDKIISTPTLDMMASQGYN
                     FENCYSPVPSCVPARAALLTGLDQETSGRVGYEDEVPWNFKNTLPEVFKEQGYQTECI
                     GKMHVYPSRKRLGFDHVLLHDGYLHVDRKYDKSYGEQFEYSSDYLMFLKESLGSDADL
                     IDDGLNCNSWEARPWMYPEKFHPTNWVVSEGINFLRRKDPTVPFFLKLSFEKPHAPLN
                     PPKYYFDMYMDRLPDTLDLHIGNWEKLEHVVPDVCALRGKLKEDDQRRMLAGYYGLIS
                     HIDHQINRFLMALKEFRHDKDTIIWFISDHGDQLGEHYLFRKGYPYQGSIRIPSFIYD
                     PGDLISAKKHGIKELVKIQDIFPSLVDLVLGQYVNTDGKSVKQLLFGNCEGWRREIHG
                     EHSLGLDSSQYILTEKWKFIWFPVKNTYQLFDMINDPNEMKNLYYDKKYESIIYEMKH
                     KLVGYLKGREEGFVKNGQLIQIGISNIVSTLKSKK"
     gene            9938..10104
                     /locus_tag="ACG8K3_002055"
                     /pseudo
     CDS             9938..10104
                     /locus_tag="ACG8K3_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10906..>11145)
                     /locus_tag="ACG8K3_002056"
     CDS             complement(10906..>11145)
                     /locus_tag="ACG8K3_002056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145906.1"
                     /translation="IKKKFKQQFKKILTAEIDVAISQSSTKYDPAFQKLIDKCLFIIK
                     IKEKFMSAFFIKVVMIIRLLIVFFRKYGIIVLTKR"
BASE COUNT         3781 a         1598 c         2137 g         3629 t
ORIGIN      
        1 aaaaatctga gccagaatgt ggttcattct gacccagatt tgattttgca caactcatca
       61 gtccttattg acaaagagcc tattttgtta gtagatgtct ggaaacggta ctttgcacat
      121 ggtgaacagg cagtgctatt agaaaaatca tgcatattac taatgcaatt gagagtattt
      181 attctagttt cagaaaagtc acaaaaaaga gcgcttctta atgagaaagt acttctaaag
      241 ttactaatta atcgttttct ataagtggtt gataatactc gttttttata gtaattgata
      301 gccgttataa agattgatga ttagcaccaa gaagatgact gtctggaaga ccagcgtcac
      361 tttttgtggt gagaggacag tgctgacttt tgcccctaac aaaccgccaa caatcgcggc
      421 tggaatcaca aaccacaaaa tctttaagtc atagaggcca aatccagtgg tcccagcaat
      481 cgtcacgatt ttcgccaatt gagagaaaaa aatcgtacaa atcgaataga ccgtggcttc
      541 tttgatgggt aaagcaaaca tcagcatcag caaggacaca ttgatcggtc caccgccaat
      601 gcccagcaga ctcgctagaa aaccaaggac taggccacag aagcaatacc agaagttatt
      661 tttcagttgg aatccgggcc agtcgtattt ggtataaaag aaagcaaaca gcagtgtcac
      721 aacggtcaaa aagatctgaa tcatttgtac ctcatcttca ttctgaaata aaagcagcag
      781 gtgatcaaaa gcgacattgc ccaagatgcc tcctaagacg gcgccgcctg aaacccaaag
      841 gacgatttgc caatggattt tacgtccact agccaactgt cttgcggtcg aaaccagcga
      901 catggtaaac acggctacgg tagaataaaa cgagatggct gcaacagaat gggcaccgat
      961 aaaatcaaaa atgggtttga tcagaacacc gccccccatg cctgaaacgg caccaaccgt
     1021 attcgccaac acgatcacta taaaataaat gaacccaatc acacacttac cccctgtaac
     1081 aaacctaaaa tccgattaat ctgcggtaca aattcctgca aaagttgctg ataaccacaa
     1141 tagcctgtgt tatctacata gatggcatct ttcatccgtt tgcagccgcc acgacacatt
     1201 ttttgaaacg ggcaactcag acatttcttt ggcatttcgg ggcgtccaca caaaaacgtt
     1261 tttgcaatgt cctgttcaaa taactgacgt aaagtctgtt cttgaatata ccccatgcga
     1321 tactcatcca gcacataaaa atcacaagga tacacactgc catctgcttc aatgacatat
     1381 tgtacctgac agtttcctaa aatcccgcag gcagtgactt gtcgcttgat caatagattc
     1441 aaaagatcat caaaaagctt gatacttata taatgcccct ccgctaattc ttgcagccat
     1501 aactgcaaaa gctggtggta aaatcctgca aaacgttttg gtgtgagagc ataactattt
     1561 cttttttttg catctagatc atctaaacaa ggaataaact ggacaaaatc aatttgttgc
     1621 tcttttagaa agcgaaagac tttcttagcc tcttttgcca acggattggt caagacacaa
     1681 agtacattgt agtcgatctc atagtagtca aacagctgtt ttgtttgcag cactcgctga
     1741 aaagttcctc ttcctttcgg atcgacccga tgcagatcat ggtagagcgg atggccatcg
     1801 atcgagagac cgaccaaaaa atcatgttct ttcaaaaaag cacaccattt ttcattgatc
     1861 atggtcccat tcgtttgaat cgcataatgg acctgtacct gcttttgttg ttgcgctact
     1921 aaccggatca ctgtacgaaa ataattgagg cccgccatcg ttggctctcc accttgaaac
     1981 gctaatgtta attgatcgcc gtcttctaag tctgcataaa tattttcgat cattttctct
     2041 gtcacttctt ctttcatttt gccaaacgag cggacttctc gcaaggaact gacattggca
     2101 taaaagcagt agctgcagcg aagattgcag agggaagatg cgggtttgat caatactgaa
     2161 atatgtttca tagtaagaac ctcatttttt aacatagttt aattatgaaa aattgctata
     2221 acgaacaaag taaaatcaaa ttcagtagta gttaattaga taggagttga atcttataga
     2281 aattccattt tttataactt tcaacatatt ctataggttt attattttca gataagcagg
     2341 ataatttggt ttgaaaaacg acaggctcag attcatttat ttttagttgt ttacatacac
     2401 tattaggagc aggaaataaa atttcgtttg tttccctaaa gttttcttcg tttaaatcaa
     2461 tgttgtagtc tgattttaaa cgtttgtaaa tagaattgta ataattaatt tctggataat
     2521 tagaatttat atattgttct ggcaaataag atttatgata gatataaggt atttcattag
     2581 cttctctaat tcgttcgatt tgataataat atgaatcagt tgataatttt aatgtatcta
     2641 atatttgttt tttattttcg cgtttaatcg aaagaacgtg tgttttatca ttgtcaaatg
     2701 aaaatatttc tatatcagaa aattttacta ttttttcttt tcttgcacgt gaaacgaaag
     2761 ttcctattcc ttgataacgt acgatatacc cctcggcaac taattctttt aacgcacgaa
     2821 ttgctgttat agaactaaca ttatattttt tgataatttc tgattctgaa tagaatcggt
     2881 cgccattatg aaattgaccg gttacaattt gttgttttag attctgtttg atttctagat
     2941 atttcggttt tattataagc tcccccttta aattactcgc atctatatta acatacttaa
     3001 aaaaaaattg ttgactttta atacaaaagc attaatatac taagtatatt aacacattga
     3061 ggtgaaaagt aacagatgac tacatttgaa ataaaggaag aatttttatt aaagggaaaa
     3121 acatttaaaa tactatctgg tgcaattcat tattttcgga ttcccccatg tgattgggaa
     3181 cattctttgt acaatttaaa agcactagga tttaatacag tagagacata tgttccttgg
     3241 aatttacatg agcctcaaaa aggagagttt cattttgaag ggatattgga tttagagaga
     3301 tttttgacaa tcgctcaaga cttaggatta tatgctattg tgcggccatc gccttatatt
     3361 tgtgctgaat gggaatttgg agggtttccg tcatggctac tccgtgaacc gattcatatc
     3421 cgaaggaatg aaatcgctta tttggagcat gtagcggatt attatgatgt tttgatgaaa
     3481 aggattgttc cccatcaact taataatggg ggaaatatat tgatgattca aattgaaaat
     3541 gagtatggat cttttgggga agaaaaggaa tatttgcgtg cgataagaga tttgatgatt
     3601 aagcgaggag tgacagttcc ttttttcact tcagatggac cgtggagggc tactttacga
     3661 gcgggaagca tgattgaaga tgatattttg gttactggta attttggttc taaagcaaaa
     3721 gacaacttca actctatgaa gcagtttttt aaagaatatg ataaaaattg gccactgatg
     3781 tgtatggaat tttgggatgg ttggtttaat cgttggaaag aaccaattat tcaacgagat
     3841 ccacaggaac tcgctgaagc ggtaaaagag gtattagaac aaggaagcat taatttatat
     3901 atgtttcatg gaggaacgaa ttttgggttt atgaatggtt gttcagcaag aggagtgatt
     3961 gatttaccgc aaattacttc ctatgattat ggtgcaccat tagatgagca ggggaatcca
     4021 acagaaaaat attatgcatt gcggaaaatg attcatgata attatccaga gattaagcag
     4081 ctagatcctg tcattaaacc cacgattgaa aagaaaaaga tttctttgac gaataaggta
     4141 agtcttttcg cgactttaga taccattagt caaccggttc aatcaaagta tccaaagaca
     4201 atggaagagt tgggacaaaa tacaggttat ctactttacc gaacaatgat agagagggat
     4261 gcagaggaag aacgattacg ggtaattgat ggcagagatc gcagccaatt atttttgaat
     4321 caagaattac aagctaccca atatcaaaca gaaataggtg aagatataca agttagtatg
     4381 acagaagaaa tcaatcaagt ggatattttg attgaaaata tgggacgtgt caattatgga
     4441 cataaactct ttgcggaaac gcaaaaaaaa ggaattagaa cgggagtaat ggcagactta
     4501 cattttatta caaattggca acagtattca ttgcctttgg aaaattgtaa aactgtggat
     4561 tattcaaaag gatggcagcc ccaacaccca agtttttatc gatatgagtt tcttttagag
     4621 gatatcggtg attgttttat tgatttaagg aaatttggta aaggaattgt ctttgttaat
     4681 cagaccaata taggtcgttt ttgggaagta ggtccaacgc tttcactgta catctctagc
     4741 ggctttctaa aagttggtaa gaatgaaatt gttatttttg agacagaagg tacctatgaa
     4801 ccagaaatca atttattaaa agcaccatta tataaagaaa tgaaagaggg atgaacaatg
     4861 agtattattg gagtaagaat tgatggacgt ttaattcacg gacaggtagc taatttatgg
     4921 acaaccaaat tgaatatttc tcgaattatg gttgtggatg atgaagtggc tcaaaatgat
     4981 attgaaaaaa gcggattgaa attggctacc ccagcagggg tgaaactaag tattttgccg
     5041 attgaaaaag cggcgcaaaa cattttagca ggaaaatatg attcacaacg tttactgatt
     5101 attgcacgaa aacctgatcg attattaaaa ttggtagaat tgggcgttcc aataaaagag
     5161 attaatgttg gaaatatgtc acaaacttct gagactcgat caattacgaa atcaattaat
     5221 gttgtcgatc aagatgtaga aacgttcaat caattaaatg aaaaaggtgt ccatttaatt
     5281 gcgcaaatgg ttccaagtga caaagcagat gactttatgt ctctgttaaa taaataataa
     5341 tatacgagga ggtgaaaaga tggcggggca tttagtttta attagtcatg gacgattttg
     5401 tgaagagtta aagagaagtg ttgatatgat catgggagta caaaaagata tttatactgt
     5461 agggttatta cctgaagagt cagcaaaaga ttttcgagta aatttcgaaa aaacaattaa
     5521 tggtttgaaa aaagttacag ttctggctga tctaatggga ggcactcctt gcaatgttgt
     5581 ttctcaatta attttagaag gaaaagaaat agaactatat gcaggaatga atatgccaat
     5641 ggttataggc tttgttaata atcaattttt aggagaagat agttctttgg taaataaagg
     5701 aagagaaaat attatacatg taaatgaata tttggaagta aatccaatag atttagaaga
     5761 tgaatgaatg tggaggaatt tatgaatata aaaaaatcca aatatatttt attgcaactt
     5821 gctgtttgat cagtttatct atgttaacaa aagtcgctca tgctgaaagt aaccccattg
     5881 gtaattgtta ctatgttagt tcgcaaagag ggagtgacac taattcagga ttatctatat
     5941 ctgagtcttt taaaagttta aataaaataa atgaacttaa ataaaatcca ggggataaag
     6001 ttttattaga aaatggattg gtttttgaag atcagtattt acaaataaaa aattcagaaa
     6061 atatagaggc gtgtattgaa atcagtaata gccaagagtc gaatatattt gtttctcctg
     6121 aaaatggtcc cgtaaaacct aattttaact atttgacata tgatcgattc agtcagaata
     6181 cagtgtttga tggatataaa ttggaacaat catctcctgc tattcatagt gggaagaagg
     6241 ttatcgataa aaatggttat aatttaggaa ctgatttttt tggtattaag ttagatggca
     6301 tactagatat tggggcagtg aaaagttcaa aataatataa aggagttagt ttgatcatga
     6361 atgatatttc tttttttcag atattttctt taatatgttt agctatacta ggaggtatag
     6421 atccttatct gtcaggatta caattaagta aaccggcaat ttctggtttt ctagcaggaa
     6481 ttataatggg agatattaat acaggcttat tagtaggagc aacattacaa ctgatggtct
     6541 taggtgtagg aacatttggt ggctcatcaa ttcctgattt tggcacaggg gcaatcattg
     6601 gtacagcatt aggtgttgtt agtggaaaag gaattgaatt tgcaataggt atttctgttc
     6661 ctgtaggctt attattagtt caattagata ttttagctcg tttttgtggg gtttattttt
     6721 tacatcgtgt ggataaacat atagaagaag aaaattttca taagattagt ttagaagcat
     6781 ggttaagttt agttcctatt gcattatctc gtgcaatacc agtaggttta tgtttaattt
     6841 ttggtaatga agtagtaaat acagtattaa attatgcacc ggattggtta atgggaggat
     6901 taaaattggc aggagctata ttaccagttg ttgggattgc cattttacta cattatttgc
     6961 caattaggaa ttttttcggt tttttaatta ttgggtatat tttagccgct tatttaaata
     7021 ttccaatgat gggtatttct tttgcaggat tggcagctgc tattattcac tataaaaaat
     7081 tacaagataa acgtttatta gaatcaaaac tagatactat caatcatata gaagtagtta
     7141 acggagaggt tgatgatgat gaaatctaat gttagcaaat taagtagcaa agatctgaga
     7201 aaagttagtt taagatatat gtttggagct caactaggtt ggaactacga acgaatgatg
     7261 aatgtggcat atgttcgagc tgtatatcca gctttgaaaa agttatacaa agatgaaaaa
     7321 gaattaaaaa gtgtgttgca atcagaaatg caattctata ataccagtcc ttttttaagt
     7381 gcatttatta ttggaattga tttagcattg caagtagaag atggaagcaa atcgaaagat
     7441 gctgttgcag caattaaaac gagtatgatg ggaccttttg cagctgtagg cgattcttta
     7501 tttggggcag tgattccaac aatttttgga tctctagcag cttatatggg attagaagga
     7561 aatccattag gtgtactact ttggttatta gtatctctca ttatactatt tttgagatat
     7621 tttgagttac caattgcttt ccgagaaggt aaaaaactgg tatccagtgt aggtggattg
     7681 ttgaaggatt taactgaatc agcaacttta cttggcgtag tagtaatagg aggcttggtt
     7741 cctagtgttg taaaggttgg tgttccattc aagttagtta ttggtgataa gaaactatct
     7801 ttccaaacag aaatgttaga ccctattatg ccagctatag tacctgttgt tttagtttta
     7861 ttggcatatt ggttattaag caaaaaatct ctcaattcaa ccaaagttat ttggatattt
     7921 ttagttttgt caatactaat gtattcatta aatattttag cagttttgta atagatgtgg
     7981 gggcgtgatc aactaattta tggcatgtcc cctgtttaat tttctaaata ttatattttt
     8041 aaatgagtgg aaagaagata ttagagattt aatgaataat aagttatata gataaaaaat
     8101 taaagatatt ttgatatcaa ggtttatttt agtataaagt attcgtgact aatcaatcat
     8161 acgctataaa gtttggtaat ccatttatat ttcttgttat cacaaaaaat atattctttc
     8221 aaaaggaaat atcaatttct taaatcaaga gagattattt ggatattttg aatgattgga
     8281 gtttttaata tgaaaaagaa tcaacccaat gttgttttaa ttgtagtaga tcagatgaga
     8341 gcagatgcgt tatctttgaa tagccaagat aaaatcatta gtacgcctac attagatatg
     8401 atggctagtc aaggatataa ttttgagaat tgttattcgc ctgttccaag ttgtgttcca
     8461 gcaagagcag cgttattaac aggattagat caagaaactt cgggaagagt gggttatgaa
     8521 gatgaggttc catggaattt taaaaatact cttccagaag tttttaaaga acagggatat
     8581 caaactgagt gtattgggaa aatgcatgtg tatccttcaa gaaaacggtt gggatttgat
     8641 catgtactat tgcatgacgg gtatcttcat gttgatcgaa aatatgacaa atcttatgga
     8701 gagcagtttg aatattctag tgattactta atgtttctaa aagaatcatt gggctcagat
     8761 gcagatttga ttgatgatgg gttaaattgt aattcatggg aagcacgacc atggatgtat
     8821 cctgaaaaat ttcatccaac aaattgggtg gtttcagaag gaataaactt tttaaggcgg
     8881 aaagatccta cagtaccatt tttcttaaaa ttatcatttg agaaacctca tgctcctttg
     8941 aatccaccaa aatattattt tgatatgtat atggatagat taccagacac tctagatttg
     9001 catattggta attgggagaa attagaacat gttgttccag atgtttgtgc cttaagagga
     9061 aaattaaaag aggatgatca aagaagaatg cttgctggat actatggcct tatttctcat
     9121 atagatcatc agatcaatcg ctttttaatg gctttaaaag aatttagaca tgataaagat
     9181 acaatcattt ggttcatttc agatcatgga gatcaattag gagaacatta cttattcaga
     9241 aaagggtatc cttaccaagg gagtattcgt atcccgtcgt ttatttacga tcctggtgat
     9301 ctgatctcag caaaaaaaca tggaattaaa gagcttgtaa aaatacagga tatatttcct
     9361 tcattagttg atttagtatt aggacaatat gtaaatacag atggaaaaag tgttaaacag
     9421 ttgctctttg gaaactgtga aggatggcgg cgagaaattc atggagaaca tagtttaggg
     9481 ctggattcta gtcaatatat attaacggag aaatggaaat ttatatggtt tcccgtaaaa
     9541 aatacgtatc aattatttga tatgataaat gatccaaatg agatgaaaaa cctatattat
     9601 gataaaaaat acgaatctat tatttatgaa atgaaacata aattagtagg atatttaaaa
     9661 ggtagagaag aggggtttgt caaaaacgga cagttaattc aaataggaat ttcaaatatc
     9721 gtttcaactt taaaaagtaa aaaataaact aaaagtgaag atgcaataaa aaatcaacat
     9781 agggtatact gagtcaaaat atactcagat acaggcaaaa ctttgcgtct ttatttattt
     9841 caaaaaaatc aaatattcgg atggtaacaa acatactact ttgtttggga taaaatcaga
     9901 tctgagtcag agtaagttca ttctgattca gttttttatg tctaacgacg ataaaagttt
     9961 agcacataca agatggaatt gtaagtatcc catagtcttt accccggagt atcgatgaaa
    10021 agtgatttat ggaaaattaa gagtaagtaa ctgttggtga tagtatcacc ccaaattagt
    10081 atttccagtt ttatgagcta tttattaaat aaattctata cgggatttga agatcctgaa
    10141 ggtgaaatcg tcatttatca gaataaataa atcaaatagt aaaagttcaa agaatgaggg
    10201 gaacattagt ttttattgct tagtttaatc ccagtaacga tttctgcaag atgtaaaagg
    10261 tctttcattt cataagtttt cagaagcaag ttcatacgca ctaactcact ttcgattgag
    10321 tagcaatcgt caacaaactt tactatcagc tgttattttt attctttcgc gttatctaaa
    10381 taccatcaat ttataagttg taggtgtatt taataaaaga acaaataact ttagtatttt
    10441 tattaatcta acttacaaac tagtagtata gggtatagta aaaatatgat aaaataaata
    10501 gtcgttatct taatccaact atatagaaaa tttacgtttt ctatatagtt ggatgtttac
    10561 aagttatatc tggtttaata agtttttttc tgttagtcat ggcatgtttt attgtgctgg
    10621 ttcatcaata tattgaaatg tactaaaatg aaattttcag ttatggtata atatttatgc
    10681 tgtctgctac cccggcctat tatggcaagg ggaggaggtt gtattatgaa ttaccttttc
    10741 gatatgtttt ttgcaatctt agcagaagta tccgcgcatt acatctgtaa atggcttgat
    10801 tgcaaaatta ttgccaaaaa agacagtaag cctacccgtt aatctgaata gaacgtaaaa
    10861 gaaaaccact agcccgccag ctagtggttt ttggtgtatc atgaattacc ttttcgttaa
    10921 aacaattata ccatatttcc taaaaaatac aattaataga cgaattatca ttaccacttt
    10981 gatgaaaaaa gctgacataa atttttcttt tatttttata atgaataaac atttatcaat
    11041 aagcttttga aaggcaggat cgtactttgt tgatgattga gatattgcta catcaatctc
    11101 agcagttaag atttttttga attgttgctt gaattttttc tttat
//
LOCUS       DAZKCN010000082        11085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000082 DAZKCN010000000
VERSION     DAZKCN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.084"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..1130
                     /gene="pplA"
                     /locus_tag="ACG8K3_002057"
     CDS             195..1130
                     /gene="pplA"
                     /locus_tag="ACG8K3_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP9145907.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1220..2323
                     /locus_tag="ACG8K3_002058"
     CDS             1220..2323
                     /locus_tag="ACG8K3_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP9145908.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2336..3289
                     /gene="menA"
                     /locus_tag="ACG8K3_002059"
     CDS             2336..3289
                     /gene="menA"
                     /locus_tag="ACG8K3_002059"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9145909.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4103..5449)
                     /gene="gor"
                     /locus_tag="ACG8K3_002060"
     CDS             complement(4103..5449)
                     /gene="gor"
                     /locus_tag="ACG8K3_002060"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9145910.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5583..6836)
                     /locus_tag="ACG8K3_002061"
     CDS             complement(5583..6836)
                     /locus_tag="ACG8K3_002061"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9145911.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6842..7765)
                     /locus_tag="ACG8K3_002062"
     CDS             complement(6842..7765)
                     /locus_tag="ACG8K3_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9145912.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7986..10478)
                     /locus_tag="ACG8K3_002063"
     CDS             complement(7986..10478)
                     /locus_tag="ACG8K3_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP9145913.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10485..10952)
                     /locus_tag="ACG8K3_002064"
     CDS             complement(10485..10952)
                     /locus_tag="ACG8K3_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP9145914.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3267 a         2263 c         1942 g         3613 t
ORIGIN      
        1 tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt cacgaaattt
       61 gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt tgacttatag
      121 gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta gaaatggaat
      181 ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt
      241 cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac tcttcttctg
      301 aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct gcaaaagttg
      361 tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat tattcaaatg
      421 gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa tcaaaatatg
      481 ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg
      541 aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac caattcttaa
      601 aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca tctgaaagct
      661 tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca gatacaatcg
      721 aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa aagaactact
      781 ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta actgaatcaa
      841 actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa tataatgaaa
      901 atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg aacgacgaat
      961 tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt gttacaggtg
     1021 ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat gcagctgaaa
     1081 aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa tcacataatc
     1141 atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc tcacctttca
     1201 aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca cactgggcat
     1261 ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc
     1321 agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt tagggactta
     1381 cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg
     1441 agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt
     1501 gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca cgttagtcaa
     1561 gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga ttggtgcaat
     1621 cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag aagaaatcga
     1681 tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag aaaaaactgt
     1741 ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag gttacattac
     1801 ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg ttgacttggg
     1861 cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact ggacagtcgg
     1921 catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag aacggaacaa
     1981 aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca aaaaatacca
     2041 tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat
     2101 cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt caatgggtgt
     2161 gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag cgatcttcgt
     2221 gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca aattatcgga
     2281 ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac gaaccatgac
     2341 actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg tatttccttt
     2401 tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc taaacacttt
     2461 tttatttttt attggaatgc tagtttttga tttagcgact accgctatca ataattacat
     2521 ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca ttggccgcag
     2581 aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat ttgtttttgt
     2641 tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg gatttatctg
     2701 ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa tgcctttagg
     2761 ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg tttatgtcaa
     2821 tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac aatttgattt
     2881 ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct tggtgtttac
     2941 gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata ttgaaaatca
     3001 tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat tccagctatt
     3061 gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct atgaatggcc
     3121 gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc aattcaagca
     3181 aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct tattcaatgc
     3241 cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac agcatgaaga
     3301 ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa atagctcctt
     3361 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatca
     3421 ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt
     3481 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
     3541 cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt
     3601 cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt
     3661 tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt
     3721 gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt
     3781 taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct
     3841 gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt
     3901 gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact
     3961 tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca
     4021 aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc
     4081 tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat
     4141 ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa
     4201 aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg attctttctt
     4261 cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt
     4321 acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg
     4381 cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa
     4441 caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc
     4501 taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa
     4561 aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca
     4621 attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa
     4681 aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat
     4741 tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt
     4801 acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc
     4861 gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt tcagccgcaa
     4921 tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat
     4981 ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag
     5041 cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga
     5101 taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag
     5161 caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat
     5221 tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg
     5281 tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca
     5341 attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag
     5401 caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc
     5461 ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg
     5521 taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt
     5581 ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt
     5641 aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact
     5701 tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca
     5761 gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact
     5821 tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca
     5881 gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg
     5941 tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc
     6001 gctttataga catttgacac cgtagataca taatggatcg atttcattct tccttcgatc
     6061 ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc
     6121 actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct
     6181 tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca
     6241 cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata
     6301 cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc
     6361 acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag
     6421 aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct
     6481 ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata
     6541 ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg
     6601 accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca
     6661 gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca
     6721 atatatactg catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct
     6781 ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc
     6841 cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag
     6901 gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt
     6961 ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg
     7021 gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt
     7081 cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa
     7141 tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt
     7201 tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat
     7261 ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg
     7321 ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg
     7381 cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta
     7441 tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca
     7501 catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag
     7561 gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta
     7621 ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt
     7681 caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt
     7741 ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata atagtagcat
     7801 agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca
     7861 caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa
     7921 aatcttgtca caacctctta atcaagtctt actattataa aaattcagct acacaggcca
     7981 agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta
     8041 ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc
     8101 tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat
     8161 tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga
     8221 atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg
     8281 atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca
     8341 ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta
     8401 gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata
     8461 tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt
     8521 aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc
     8581 acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca
     8641 ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt
     8701 tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct
     8761 gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa
     8821 ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact
     8881 gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac
     8941 aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca
     9001 actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca
     9061 attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca
     9121 gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt
     9181 tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct
     9241 gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga
     9301 acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct
     9361 tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct
     9421 acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat
     9481 tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag
     9541 atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca
     9601 tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga
     9661 tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc
     9721 aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct
     9781 tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc
     9841 cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc
     9901 agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt
     9961 ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt
    10021 ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg
    10081 ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc
    10141 atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct
    10201 aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa
    10261 cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa
    10321 tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa
    10381 acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct
    10441 aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca
    10501 taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca
    10561 ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta
    10621 attatttggt cttcataaag tttttgaata atagcaaaag catttttctc actgattgta
    10681 tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt
    10741 gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg
    10801 gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc
    10861 atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat
    10921 tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga
    10981 ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc
    11041 ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DAZKCN010000083        10754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000083 DAZKCN010000000
VERSION     DAZKCN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.085"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8K3_002065"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG8K3_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9145915.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG8K3_002066"
     CDS             1995..4661
                     /locus_tag="ACG8K3_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9145916.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG8K3_002067"
     CDS             5025..5777
                     /locus_tag="ACG8K3_002067"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9145917.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG8K3_002068"
     CDS             complement(5799..6953)
                     /locus_tag="ACG8K3_002068"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP9145918.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG8K3_002069"
     CDS             7138..9579
                     /locus_tag="ACG8K3_002069"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP9145919.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="ACG8K3_002070"
     CDS             complement(9909..10613)
                     /locus_tag="ACG8K3_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145920.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3432 a         1931 c         2266 g         3125 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaa
//
LOCUS       DAZKCN010000084        10687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000084 DAZKCN010000000
VERSION     DAZKCN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.086"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..709
                     /locus_tag="ACG8K3_002071"
     CDS             116..709
                     /locus_tag="ACG8K3_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP9145921.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            725..1624
                     /locus_tag="ACG8K3_002072"
     CDS             725..1624
                     /locus_tag="ACG8K3_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP9145922.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERINEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            1627..2112
                     /gene="ssb"
                     /locus_tag="ACG8K3_002073"
     CDS             1627..2112
                     /gene="ssb"
                     /locus_tag="ACG8K3_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9145923.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2157..2390
                     /locus_tag="ACG8K3_002074"
     CDS             2157..2390
                     /locus_tag="ACG8K3_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145924.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(2549..2671)
                     /locus_tag="ACG8K3_002075"
     CDS             complement(2549..2671)
                     /locus_tag="ACG8K3_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFP9145925.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2826..3086
                     /locus_tag="ACG8K3_002076"
     CDS             2826..3086
                     /locus_tag="ACG8K3_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145926.1"
                     /translation="MDKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3529..3735
                     /locus_tag="ACG8K3_002077"
     CDS             3529..3735
                     /locus_tag="ACG8K3_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP9145927.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKKH"
     gene            3735..3986
                     /locus_tag="ACG8K3_002078"
     CDS             3735..3986
                     /locus_tag="ACG8K3_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145928.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4002..4439
                     /locus_tag="ACG8K3_002079"
     CDS             4002..4439
                     /locus_tag="ACG8K3_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145929.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(4550..4786)
                     /locus_tag="ACG8K3_002080"
     CDS             complement(4550..4786)
                     /locus_tag="ACG8K3_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145930.1"
                     /translation="MKQNTSITKNTTKFKCTAALCRIIISPTNSTNNKGIILFKWITF
                     FLLSYRALPVYFASPKRRGRLERLVRNKNHSLSY"
     gene            4884..5063
                     /locus_tag="ACG8K3_002081"
     CDS             4884..5063
                     /locus_tag="ACG8K3_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145931.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5404..5667
                     /locus_tag="ACG8K3_002082"
     CDS             5404..5667
                     /locus_tag="ACG8K3_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFP9145932.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            5661..6011
                     /locus_tag="ACG8K3_002083"
     CDS             5661..6011
                     /locus_tag="ACG8K3_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9145933.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(6035..6649)
                     /locus_tag="ACG8K3_002084"
     CDS             complement(6035..6649)
                     /locus_tag="ACG8K3_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9145934.1"
                     /translation="MRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRP
                     VFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            complement(6990..7193)
                     /locus_tag="ACG8K3_002085"
     CDS             complement(6990..7193)
                     /locus_tag="ACG8K3_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145935.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(7203..7625)
                     /locus_tag="ACG8K3_002086"
     CDS             complement(7203..7625)
                     /locus_tag="ACG8K3_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145936.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            complement(7794..8984)
                     /locus_tag="ACG8K3_002087"
     CDS             complement(7794..8984)
                     /locus_tag="ACG8K3_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9145937.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            complement(9383..10684)
                     /locus_tag="ACG8K3_002088"
     CDS             complement(9383..10684)
                     /locus_tag="ACG8K3_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9145938.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT         3620 a         1775 c         1969 g         3323 t
ORIGIN      
        1 aaccagcagc ggattcaaaa agaaattgaa ttagatcgat aaatttcttt cttgttaata
       61 taaaactaac atgttatagt tatgttagtt ttatattaac tttaggggaa aagcaatgaa
      121 agacaaaatc cagtgtattt ttgataaaca taatgggaat ctatccttga aggatgctag
      181 aagtgaagga ttatcgccta tgacgttaga tcgattagcc gaaaaggaca aaatagaaaa
      241 ggtagcacca ggtttctata ccttaaaaga agcgttcgtg gatgaattgt atctcgctca
      301 aagtatctat tccagaggga ttttttctca tgagactgcg ctcgatcttt ttcaagtagg
      361 aacgtatatc cctaaaaaga tcaatatgat gtttcctaaa ggatacaatg tttcaaagga
      421 gaaattagac gagtatgcta ttcaaccgca ttacacgaat aaagaaaatt ttgacctggg
      481 aatcacagaa acagaaagct tttacggcaa taaaatttta gtctatgatt tagagcgaac
      541 gttatgtgac atgtggaatc caagatacaa agcaagcgta gaggtaaaac aagaagcatt
      601 aaaagaatat atgacaagta ccagtagaaa tacaaataag ctaagaagat acatggatat
      661 tttaaaatca ccaaaagaaa tgactttgta tatgttgcca ctttattgag aggagtaaag
      721 gtgaatgaca ccgacgcaat ttaaagcaaa aacgagaaat tttgcaacag agaaacattt
      781 aaacccacaa attgtccaaa gatattatat gatggaaaaa ttattagaac gtattgttga
      841 atcgccttat catgatgatt ttgtattaaa agggggattt ctgatcggat caaaatacgg
      901 gttagaaaac cgttcaactg ttgatattga tacgacttat cgaaattcga aactaacaaa
      961 agaaaagtta gaaactgttt tttctgaatt aacagctgat cccacaaaag atggaattcg
     1021 atttgaatta aagggattaa cagagacgag ggaagctgat tattacccag gttttcaagc
     1081 aaagtttatt gcttatcttg agaatgcacg tgtaccattt aaattagata ttacttcagg
     1141 tgattctatt attccagcgg cattgatgta tgaacacaaa ttaatgtttg aagataaaac
     1201 gattcatatt ccagcttatc ctacggaaca aattctagct gaaaaattac atgccacgtt
     1261 tagttttgga aaagataatt cgcgcatgaa agatttttat gacctgtata tgattccgaa
     1321 attagaacgt atcaatgaga aaaatttgta taaaagtgtt caaggaacat tcaaggaaag
     1381 aaataatcaa tcggtgttta agcatttgta tgaccaggaa atgcctcaat tggtgaagga
     1441 tgaggggttg agggaaaagt ggaaaagata ccaacaagaa aactattttg ccaaagacat
     1501 ttctttcgaa tctacactaa cttccgttga ttccctgatg caatcaattg tagagcaaga
     1561 gaaggtagaa cgaaaacaaa attttaagag atcacaacaa atgaaccaag aaatggagag
     1621 ataaagatga ttaataatgt aactttagta ggacggttaa ccaaagatcc agatttacgt
     1681 tataccgcaa gtggcacagc agtagcgact tttaccctag ccgtcaaccg aaatttcgcg
     1741 aatcaaaacg gcaatcgaga agccgatttt attaactgcg tgatttggcg caaaccagca
     1801 gaaacaatgg cgacattagc taaaaaaggc ggcttgatcg gtgttgtcgg acggattcaa
     1861 acacgaactt atgacaacca gcaaggacaa cgtgtgtatg tgactgaagt tgtagcagat
     1921 aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc tgatcaatcg
     1981 tccaccagtc caagcaccgc cacctttgaa caacgggata cagcgacgcc taacaacaat
     2041 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
     2101 ctaccgtttt agttcgagaa agaaggagta agcatggcaa aaaaaatagt ctgacgatgg
     2161 atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc actgaaaatc
     2221 ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat cttgtatgtt
     2281 cttatctttt aaaaagagca ggaagggata attccttttg gcgattgact aaagtcacaa
     2341 tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag ggttaacggg
     2401 aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag gagttgcgct
     2461 aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag ttatttggaa
     2521 ataaccgctg acctgctaaa gtggacggtt atttcttttt gtcattttta agcatattta
     2581 caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt tgaattgtct
     2641 catatgcgga caaaaggctt actcctttca tagattttag cacttacatt cataagcacc
     2701 accacctttc cagaagtcgc caccgtcttc aaactttact gtcttgacta ttatagcata
     2761 tctaaaatcg aataaaaatt ctcaattttg gttgtttttg ctataatttt agcgaggtga
     2821 ttgtgatgga caaatatgtt ggtttgcttg ataaaatccg agtaataaaa acacaaccat
     2881 tattggttcg ctttacttta caaacaattc atgaatcgat taattgtgta gttgcagata
     2941 ttgaaattat cgataaactg ctaattatgg atgatggaaa atacaatatt gccgtcacag
     3001 gtcacttcaa taaacgaaat cagctagtta ttgctagcat gtatgttaga aatcccgatc
     3061 attttacgcg atcgatgggc atataaaaga agagttgtag tcctaaatga ggattgcagc
     3121 tctttttgtt ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc
     3181 cttctcaatt gaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga
     3241 agtgaacgtt gcacgttcac taaaataagg ggtcttgcaa agcaagacaa aataggaaaa
     3301 acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg
     3361 gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg
     3421 cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga
     3481 actaacaaaa ggaagtaaac cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa
     3541 tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact
     3601 gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt
     3661 caaacatgcg ggaaaaaata taccttgtct tatggcgatt atgttttgat tgatgagaaa
     3721 ggaaagaaac actgatgaaa acacaacaca aattattgac tgtaaaagaa gtcgttcaaa
     3781 ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt attccgcggt
     3841 actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt gaagacctag
     3901 tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat gaaaacgaat
     3961 attggttaac caaattagag aactaaaaag gagaaaaaaa tatggctcaa aattactatg
     4021 acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc
     4081 tatacaacga aacacgtgtt ccaaagaaac attataaaga aaaattgtcg gtagctgtgg
     4141 aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg
     4201 aagaactgaa aaagcaaaat gcaaaatggt ttttccaagc cttactatgc ttggaagtag
     4261 gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatgcga
     4321 aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa
     4381 atatcaataa gtatggcgca tattacacca tgaaaaagga agacaacgaa aatgaataag
     4441 gtgatttatc aactaacaac ggattccata tcaacgattt gaaagaaaaa aaggtcaata
     4501 atcatttata ccgaaaagtg agaatgtgtg aaataaggac aaaaaaagcc tagtaactaa
     4561 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtgatgcga
     4621 aataaacggg caaagcccga taactcaaga gaaagaaggt gatccatttg aacaagatta
     4681 ttcccttatt gttagtacta tttgttggcg aaataattat gcgacatagt gcagctgtgc
     4741 atttaaactt cgtagtattt ttggtaatac tggtgttttg tttgatcttt tggcgctttg
     4801 tgttagccct tgtgagtcta atattcaagt tgattgcagc catatttttc atagggttat
     4861 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtta
     4921 cgttggatga aaagagacaa aaagaattgc agaaactaaa gcagaagtac ggaaaaagtg
     4981 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
     5041 atgtaccagc actcaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
     5101 ttaattgttc ctactattta gaatattttc tatatccttt ttattccatt ttgtatcaat
     5161 aactatgaaa tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc
     5221 tgattctaaa gattttgttt tcttaaacat attatgattt cctttcgact attttcatat
     5281 aaatgttaac atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa
     5341 ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct
     5401 actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag
     5461 tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa
     5521 gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt
     5581 gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt
     5641 gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga
     5701 atacagcacc tcgatctgga cacgaacaga caggtaaaag gccttatatt gttttaagtc
     5761 atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta
     5821 gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag
     5881 tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa
     5941 aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag
     6001 taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc gtgctgtctt
     6061 tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg
     6121 caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt
     6181 ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct ttcgcaatct
     6241 taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca
     6301 aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg
     6361 ccatgatcgg tagacttgta atgatcagct gcacattttt cttcttcaag taattgactg
     6421 aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat
     6481 cttgatctcg gacgtaatcc aatacttctt ggaaaacagg tcgatgagta attgtagcgc
     6541 cagattgttt ctcgatgaaa attctctctg caccgaattt tttcatttct tcaacttggc
     6601 gttgttcatt ttgatcgatc gatgagacac gtatgtatgc aactctcaaa tttttaccta
     6661 ctttctgcaa ctatttaaaa cacaccctaa tcatacatga gaaaaccaga tttttcaatg
     6721 tttgtcattt ttctagataa atggcataaa ttttcagcgc taagtgacaa aagcaactaa
     6781 tttttcatga tagattttta aatatctcaa tctatctcca aaattagttg cttttgtagc
     6841 tatagttgac tatgaatatg acaaataatt agaaaaaact gtgccaatta aatataaaaa
     6901 tgacagaaag tgtccagaaa tgggtgattt tatgaacact ttcaattaga aaaaaagaat
     6961 gtcgactaga cactcttttt ttaaaatcat tatttttctt tttgatatgt tttttggtct
     7021 cttttttttc ttttttcttc agaatcttct agattagaaa ttgatttttg aattttttgt
     7081 gtctcctgta aagttcgctg aacttgtaaa gctgattctt ctttcggtaa attaatttta
     7141 cttagaatat cgctatcttg ataaaaattt ggtgtgttca caaattctgg cataaaaaat
     7201 ccctactttc tagttaaggt tcctgaataa gtcgcaatcc aattgtttcc agctgattta
     7261 taactttgta aatacaattt tccatagtac caataaccat ttcttttttc tttccaattc
     7321 cgtgattgag gaggtctaga aggccaacca actttatcaa aaatcatcgt tcctgtcact
     7381 cgtatcgttt tagaagctgc tcgagcaagt aatggagaaa ctattaaata attttcagta
     7441 acagttaagt cgttagttgg gttaccaatg gctattccgt tcctatctaa aggagttaat
     7501 tcttggttat tatctactac agtagttggt tcatttgtac tatcagcaaa tgtaggaatt
     7561 gtagttccag ttacactaat tccaatagtt aaaataccta aagataaaat aaacttattt
     7621 ttcattgtct atcacctcga aatcaaaaat aagaaaacgt tttcttaata aatcttaaca
     7681 gataaatcaa gttatatact agaaaattca taaaatatat ttttaatgtc ataatcgttt
     7741 aaatttggga gcgtcaataa ttttgtgtaa aagtatatcc ttggtaaagc agattagctc
     7801 ttgaaccaat cttctctggt tgtttgtcca aaacctctat gtacgtgatt catgtttttc
     7861 tcgttgtaac gattgaactg aatcacaagg tatttctctt cagatttttc cgttggaaat
     7921 tgttctttct ttttgaaact tcgttttaat tgtttattgt ttccttcaat cagatttgtt
     7981 gaataaatcg tatgcctaac actttctgga taatcataga atgtaaacag atagtcattg
     8041 ttgctgagac ttttcaccat tgatggatat ttgtgtttcc acctatcgat gaatttcttt
     8101 agagaagtta aagcttcttc ttttgatttg gaccgatata cctctttaaa ttcattggcc
     8161 acttcttttc tgtcactgac tcttactttt gcacatatat ttctttgaat atggaccagg
     8221 catctttgaa ttttagagcc cgggaaaatg tcctgtatta cattttccat cccagacaat
     8281 ccgtctgtac aaaataaaga aatacgttca acccctcttg atttaaggtc tttcagaagt
     8341 tcttcccata tctcagcgga ctcgtttggt gcaatcatat agccaagaat ttcttttgta
     8401 ccttctactg tgattcctag tgctatatgg accatttccc tggatacagt atcacggcgt
     8461 aaaggcatgg atgttccatc aagatataca acggcatact ctttgttcaa tattctgttt
     8521 ttgaaggaat ctacacattc cagagctttg tccgtaatat tagatatggt ctgtttagaa
     8581 tagcgagctc cacataattt ttggatcatg gaacaaattt ctgaatggct cattccttct
     8641 tcaaataaat ccaaaatcgt tgtttcggta aatagatctc gtcttctgta tttaggcaga
     8701 agagaagtga aaaattctcc cattctatct cttggaatat tgagtgtaat agttccatat
     8761 tttgtatcaa actttctttg ataggaacca tttctagaat tttcgttatt ggttctagcg
     8821 tatggttcat aatcaagaaa atccgtcaac tcatatgcga gtatttcatt gagagatttc
     8881 tccaactctg aacgaaacag ttcggttaca gcctgttcaa aagctggaag attgttgtca
     8941 ttagaaaata ggtttttcat taaagatgta gtaaaatagt tcatgggtat attcctttcg
     9001 taaattgttt ttggtcgaac taattttact aaggatatac ctttttttct acatttctag
     9061 aaaaacgaat ttacacaaga tattttatgc tatccattta tttttgaaac tccatttgtt
     9121 ttactacgtg tattaccttt atttttcttt ctatttttag gtgtctatta tctcttacgt
     9181 tcgcttataa atgatgactc aaaatttaaa gcaaaataaa aataaaggaa gtagcctttt
     9241 gaacaagggc gcttccttta tttgttgtgg gacaccagag tatacttttt tataaaaaat
     9301 aggttgatgg gaaaccctaa gatttcccat caacactaaa tagtatagaa ccatattttc
     9361 gttacaaggt taagaatgac gactatagtt tttcatctac taattttgtt ttattgctct
     9421 ccatgacttt aaaaaatgga tagtagatga agatgctgat gacgatcaac agtaggcaaa
     9481 agagaatatt gcgccagtcc atacttgaaa gataagcttg tgcaaaaaaa ggtgtgaagg
     9541 aaggatcgac gataaatcca atcccaatcc atcctagttg ctgagcaaag taggtcagca
     9601 ataatgaaat gatgggcgta atcaaaaatg gaattgccaa aatagggtta aaaacgatag
     9661 gtaatccgaa aatcactggt tcgttgatgg aacagattcc aggaaccaat gacaattttc
     9721 caattgtgcg atattcgggt actttgcttc gcatcatcaa taaaaccaaa cccaaagtac
     9781 caccggctcc accaagtaca gaaatacgga acatttgtaa gttcattaag tgtgtcatgg
     9841 tttcaccatt tgccagttgt tcagcattca aaccagtttg agccattccc aatgtaaaga
     9901 cgatagggaa aataattgac gagccattga taccaaatag ccataggatg ttacccaaag
     9961 tcacgatcaa taagaaacca cctagactgc cagcgatgtt cgttgctgga gtcaggactt
    10021 ccatcaccgc ttcagggaag attttgtttg ttgctgccaa gaaaatcaga ttgatgccat
    10081 aaaagatgaa tatatttgtc agcagtggaa cgagcatatt gataaaggtt gctaccatgg
    10141 gtggaactgt atctgggagt ttcaacttaa tattttttac ttcaaatagt cggttgattt
    10201 ccactactag caagccgatg ataattgcga cgaacaatcc gtttgtccca aggaaattaa
    10261 gattgatggc accttcttcc actggtgtac aaaccattag ataaatgaca gatgcaacca
    10321 tgccattcat ggaaggatta attttgtatt cattggcaag ggaaaatgca atgccaaaaa
    10381 cactaatgaa tccgatgatt cccattgtca aattataagg ggctgtgatg agatcataat
    10441 ttgctacggc aaaatctttc cagctggcca taaatttcat gaaaatatta gctgtatctg
    10501 gattgtattt atcaatattg atgggtggat ttgcaaaaat cagaaaaaaa gaaccgataa
    10561 caataaaggg taggccaaac atcataccgc tggatatagc ttttaggtgg cgctgattac
    10621 ctatcttggc tgcgatgggg ctaagtatgt cattcagttt catgataaac gcagaatttt
    10681 ccatata
//
LOCUS       DAZKCN010000085        10600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000085 DAZKCN010000000
VERSION     DAZKCN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.087"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(217..702)
                     /locus_tag="ACG8K3_002089"
     CDS             complement(217..702)
                     /locus_tag="ACG8K3_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP9145939.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    792..1137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1182..1823
                     /locus_tag="ACG8K3_002090"
     CDS             1182..1823
                     /locus_tag="ACG8K3_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9145940.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1842..2477
                     /locus_tag="ACG8K3_002091"
     CDS             1842..2477
                     /locus_tag="ACG8K3_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9145941.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2495..3310
                     /locus_tag="ACG8K3_002092"
     CDS             2495..3310
                     /locus_tag="ACG8K3_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9145942.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3629..5065
                     /locus_tag="ACG8K3_002093"
     CDS             3629..5065
                     /locus_tag="ACG8K3_002093"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9145943.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5254..7701
                     /locus_tag="ACG8K3_002094"
     CDS             5254..7701
                     /locus_tag="ACG8K3_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9145944.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7931..8998
                     /gene="rlmN"
                     /locus_tag="ACG8K3_002095"
     CDS             7931..8998
                     /gene="rlmN"
                     /locus_tag="ACG8K3_002095"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP9145945.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8988..9194
                     /locus_tag="ACG8K3_002096"
     CDS             8988..9194
                     /locus_tag="ACG8K3_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145946.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9191..10294)
                     /locus_tag="ACG8K3_002097"
     CDS             complement(9191..10294)
                     /locus_tag="ACG8K3_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9145947.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3501 a         1808 c         2292 g         2999 t
ORIGIN      
        1 atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa
       61 tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg
      121 ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag
      181 acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag
      241 atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt
      301 attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac
      361 ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac
      421 gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg
      481 ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg
      541 ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg
      601 gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt
      661 ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt
      721 ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg
      781 taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct
      841 ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga
      901 agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag
      961 ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc
     1021 ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg
     1081 ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca
     1141 tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag
     1201 ttatgccgca attattagta ggagcactca caacattgaa attattttta tttaccttga
     1261 ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc
     1321 tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac
     1381 tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag
     1441 cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga
     1501 tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc
     1561 aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga
     1621 atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg
     1681 taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg
     1741 tggcaatcat ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag
     1801 gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag
     1861 tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg
     1921 ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct
     1981 tggctggctt agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc
     2041 cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat
     2101 ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga
     2161 caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt
     2221 tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca
     2281 gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc
     2341 ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc
     2401 aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag
     2461 tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc
     2521 tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa
     2581 ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc
     2641 tatgggattt agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc
     2701 agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga
     2761 atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc
     2821 aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac
     2881 acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca
     2941 aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt
     3001 caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag
     3061 cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag
     3121 aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt
     3181 tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact
     3241 agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc
     3301 gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata
     3361 aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct
     3421 tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta
     3481 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
     3541 cctttacata tttttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga
     3601 ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa
     3661 agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga
     3721 tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg
     3781 aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat
     3841 gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa
     3901 tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt
     3961 gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt
     4021 gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc
     4081 agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca
     4141 aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc
     4201 gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa
     4261 atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta
     4321 tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc
     4381 caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt
     4441 agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc
     4501 taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt
     4561 aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac
     4621 tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga
     4681 ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg
     4741 catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa
     4801 acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga
     4861 agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct
     4921 tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac
     4981 acaagaagga tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat
     5041 tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata
     5101 tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg
     5161 aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg
     5221 tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa
     5281 aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg
     5341 attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt
     5401 ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc
     5461 tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt
     5521 ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta
     5581 catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg
     5641 gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg
     5701 attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta
     5761 gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag
     5821 ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct
     5881 gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg
     5941 aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag
     6001 aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa
     6061 aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat
     6121 cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt
     6181 ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa
     6241 caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg
     6301 gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc
     6361 caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa
     6421 ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat
     6481 atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca
     6541 ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc
     6601 gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct
     6661 ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact
     6721 ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag
     6781 ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac
     6841 ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta
     6901 caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga
     6961 aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat
     7021 ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc
     7081 agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt
     7141 catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca
     7201 aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt
     7261 gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt
     7321 gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc
     7381 gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat
     7441 gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa
     7501 gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc
     7561 ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg
     7621 tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg
     7681 ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata
     7741 aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac
     7801 ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg
     7861 aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag
     7921 ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg
     7981 gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca
     8041 aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt
     8101 atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg
     8161 tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag
     8221 acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac
     8281 gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc
     8341 acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca
     8401 tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt
     8461 gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac
     8521 tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc
     8581 tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag
     8641 tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg
     8701 aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca
     8761 attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa
     8821 tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta
     8881 tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat
     8941 tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc
     9001 gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt
     9061 tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa
     9121 aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt
     9181 tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag
     9241 tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt
     9301 taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga
     9361 gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat
     9421 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
     9481 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
     9541 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
     9601 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
     9661 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
     9721 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
     9781 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
     9841 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
     9901 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
     9961 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
    10021 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
    10081 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
    10141 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
    10201 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
    10261 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
    10321 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
    10381 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
    10441 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
    10501 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
    10561 gattattata tgaaaacagg ttttatttgt caattcataa
//
LOCUS       DAZKCN010000086        10570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000086 DAZKCN010000000
VERSION     DAZKCN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.088"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..272
                     /locus_tag="ACG8K3_002098"
     CDS             48..272
                     /locus_tag="ACG8K3_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFP9145948.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(477..797)
                     /locus_tag="ACG8K3_002099"
     CDS             complement(477..797)
                     /locus_tag="ACG8K3_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP9145949.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(945..3179)
                     /locus_tag="ACG8K3_002100"
     CDS             complement(945..3179)
                     /locus_tag="ACG8K3_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9145950.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGISVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3658..3924
                     /locus_tag="ACG8K3_002101"
     CDS             3658..3924
                     /locus_tag="ACG8K3_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP9145951.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3924..5651
                     /gene="ptsP"
                     /locus_tag="ACG8K3_002102"
     CDS             3924..5651
                     /gene="ptsP"
                     /locus_tag="ACG8K3_002102"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9145952.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5815..6861
                     /locus_tag="ACG8K3_002103"
     CDS             5815..6861
                     /locus_tag="ACG8K3_002103"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9145953.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6878..8101
                     /locus_tag="ACG8K3_002104"
     CDS             6878..8101
                     /locus_tag="ACG8K3_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9145954.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            8190..9077
                     /locus_tag="ACG8K3_002105"
     CDS             8190..9077
                     /locus_tag="ACG8K3_002105"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9145955.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            9163..9393
                     /locus_tag="ACG8K3_002106"
     CDS             9163..9393
                     /locus_tag="ACG8K3_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP9145956.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9450..10415)
                     /locus_tag="ACG8K3_002107"
     CDS             complement(9450..10415)
                     /locus_tag="ACG8K3_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP9145957.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         3432 a         1840 c         2135 g         3163 t
ORIGIN      
        1 aaaaattatt ttgcgtacat atgattaaag aaaggagagt gtgacacatg gaaacagaat
       61 ggcaaagacg tgaaagaaaa aaacagaaaa aagaagagat gttacgatta tgtcgattat
      121 tagatgtaaa agactgggac aaaaacgagc aaattgcaca aaaagtacga tcaatcatcc
      181 ataaagagga aaaagttaaa aataaagaca cgcgtagaac tgaaaatgaa aagagaaaag
      241 tacaaaagaa aagagaaaaa tcaaaaagct agaaagaaaa agaggctgag acagaagcgt
      301 ttaactccaa gaaataagct ggaattcaca aaaatttgaa aagcaatttt cgtgaatttt
      361 agcttatttc cgaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga
      421 caaaagtgtt ctttactttt gtcccacgct ctttaattat tatgttcaaa atttgtttac
      481 acggctaatg tagcgcctaa ttcatgtttt ggaatcgaga tttcaacaag gcctgccaat
      541 tcgattactt tgatttcatc ctcgtggtaa acggcgtctt tgattttatc taagaaataa
      601 tttttagcat cttcgatttc tttcggctgg attttacgtt tgtcattgat cagcagaatt
      661 tcattatgtt caggggcttc agtgtagaca atagtggtat ttccggctgt gtagaccttg
      721 actagatggg catcagtgtt ttcaagctgg tttaatacaa gacgggaatg gctgtttgtt
      781 acatttacca atttcatggc gctcacctct tttatgtatt tttaattgct tgtttctctt
      841 attataattt cttttttata aaatgaacag tattttgact tttctgaaaa aagaatgaga
      901 cggcatttct caaagaaata ccgtctcacg ctataaaact ggatttattc agccgtttct
      961 tcggctgatt ctttagccaa acgttctggt ttagaagtaa cgatgatttt tccatcttta
     1021 tccagttttg ctttcaactc atggatagaa gggtgatcaa gataaaattc agcgatacca
     1081 tcttcgattt gttcttggat cgtgcgtcga agaggacgag cacccataga tggatcatag
     1141 cctaagtcaa ctaatttttc tttgacattc gtaggaactt cgatgtgaag ttgctgtgct
     1201 gccagcattt ggttgacatc gtctagcatt agtgtgacga ttgtcatcaa gttttctttt
     1261 gataatgcac tgaattcgat gatcccgtca aaacggttca gaaattctgg tgtaaagtag
     1321 ttgtttaatt ggttcaatac agaacgagta acaccttcgc gtgccgcacc aaaaccaacg
     1381 tttgcttcta ctttaccagt tccggcatta cttgtcataa tgatgatcgt atctttaaag
     1441 ctgaccgttc ttccttgtgc atctgtcaat cgaccatcat ctaagatttg caagaacata
     1501 tgcaaaacat ctggatgagc tttttctact tcatctaaca agacaagact gtaaggattt
     1561 ctccgtactt tttctgttaa ttgaccagct tcttcgtatc cgacataacc tggaggagaa
     1621 ccaatcaatt tagagacact atgtttttcc atatattcag acatatcgaa acgaatcatt
     1681 gaatcttgtg aaccgaatag ttcgtaggca agttgtttcg caagctctgt tttacctaca
     1741 ccagttggtc caacaaataa gaaggagccg attggacgat tctgtttgtt taatccaaca
     1801 cggtttcggc gaatcgcttt cgctactcga tctacagcgt tatcttgtcc gatgacatga
     1861 gctttcaagt catctgctaa gtttttcagt tgtgtctgtt ctttttcttt taattcaccc
     1921 actgaaatac ctgtccgttg ttcaacgatt ttttccatat ctttttcggt aatcacaggt
     1981 gtttcctctt cagtgagctg acgttctttc attttttgaa gtttattgat ctgatcgcga
     2041 taataagctg ctttttcgaa atcttcttct cgagaagcta aaactttttg ttcttctgct
     2101 tcttgcagtt ttttctcgat tgttttagga tcaacgattt ggatcgtcaa gtttttctta
     2161 gaacctgttt catctagcaa atcgatggct ttatccggca agaagcgatc ttggacgtaa
     2221 cgattggata aagttgcggc tgcttcgatg gcctcatcag tatattttac atgatgatag
     2281 tcttcataac gtttttgcaa acctttcaaa atactgatcg tttcttcgac agttggttca
     2341 tctacccgta caggttgcat acgacgttct aaagcagcgt ctttttcgat gatgcggtac
     2401 tcatttaagg tagtagcacc aaccatttgc aattctcctc gagctaaagc tggttttaaa
     2461 atattaccag catccatatt tccatcacca gcagaaccag caccgacgat ttcgtgaact
     2521 tcatctatga ataggatcac attttctgct tgacggatct cttcaatcaa tttttgcata
     2581 cgttcttcaa attgtcctcg tataccagta ccttggacta aagatacgac atctaaacga
     2641 atgacttctt tgtccattaa tttttgcggt acatcaccat ctacgatttt ttgtgctaaa
     2701 ccttcaacaa ctgctgtttt accaacacct ggttctccaa tcaagacagg attgttcttg
     2761 gttcggcgat ttaaaatttc aatgactcgt ttgatttcgt catcgcgtcc gatcacagga
     2821 tcgatatcac cttgtcgtgc ttgttctgtg atattgattc cgtattctcc caataaacct
     2881 tctgtattgc cttgaggtgg ctgaccacca ttgaagttgt tgtttccgcc aaattgtgtt
     2941 ggtggtgttt gctcataatt tccttgctgt tgcatttggc gagacatgga acggaaaaga
     3001 tcgtccaaac tgccaaatcc aaatggatca ttttgggcca tattagtcag acctccattt
     3061 tgttgatttt tgattttttg ataacagctc tgacagtaat cgagttgtgt ccgctgacca
     3121 ttgacagtgg catacaagtg aatcgttgcc tcattcttgc cgcagttttg acaaagcatg
     3181 aatcattcac gtcctttcac aaaaatgggt acatccatat tttacatctc gtttttcatg
     3241 aggtaaaaga ataaccccta gttttttaag agatttatga ggtagaaaag gtgaaatctc
     3301 tattgaccat tcctgacctt tgactcttat tatactaaaa ataaaaaggg aagcaagtaa
     3361 ttcgtttcat atgaaaacaa agagggtcaa aggaaagaat gataaacgtc aggagaagag
     3421 aaagcgacaa gaaaaagaaa ataaacacgt ttgttataaa atgtgaagaa aaggatgaaa
     3481 aagaatccgg aataatttga atacttttat ctgaaattga atgaaaactg ttgtatctaa
     3541 cgaaaaaaaa tgttaatctt tacagatgaa agggtggcat ttgttaggat tctctgaatt
     3601 actaaaaaag aaaaccctca cataaaaaca acactcactt aaaggagacc gattaatatg
     3661 gaaaagaaag aatttcacgt agtagcagaa actgggatcc acgctcgtcc agctacacta
     3721 ttagtacaaa cagcaagcaa atttaactct gatgtaaact tagagtataa aggtaaatca
     3781 gtaaacttga aatcaatcat gggcgttatg tctttaggtg taggtcaagg ttctgatgtt
     3841 actattactg cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt tgaaacaatg
     3901 aagaaagaag gcttatctga ataatggttg aaatgctaaa agggattgcc gctagtgacg
     3961 gtgtagccgt tgcaaaagct tacctgctag ttcaaccgga tttaacattc agtaaggcaa
     4021 cagtagaaga tactgcggct gaagaagctc gtttggatgc tgcattagct aaatctactg
     4081 aagaattgca gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca gaagcgcaag
     4141 tatttgatgc ccatttgatg gttctttcag atccagaaat ggtaggtcaa atcaagcaaa
     4201 atattaaaga caacagcgta aatgctgaat cagcgctaaa agaagtaacg gatatgtata
     4261 tcggtatgtt cgaagcaatg gaagataatg cttatatgca agagcgtgct gcagatattc
     4321 gtgatgttgc taaacgtatt ttagcgcact tattaggcgt tactttacca aatccatcaa
     4381 tgatcaacga agaagtggtc gttgttgcac atgacttgac tccaagtgat acagcacaat
     4441 tagatcgcaa ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact tcacattctg
     4501 ctatcatggc acgttcttta gaaattccag caatcgttgg tacaaaagaa attactgcta
     4561 aagtcaaaga aggcgatatt ttggctgtca acgggatcga aggcgatgta atcatcgatc
     4621 ctacagatga acaaaaagct gaatttgaaa aagcaggtgc agattacgca gcacaaaaag
     4681 cagaatggga aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag cattttgaat
     4741 tagctgcaaa catcggaaca ccaaaagatc tagttggtgt ccataataat ggtggagaag
     4801 cagttggtct gtaccgtact gaattcctgt atatggattc tccagacttc ccaacagaag
     4861 atgaccaata tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa cctgtagttg
     4921 ttcgtacaat ggacatcggt ggagataaag aactaccata tctgcaatta cctcatgaaa
     4981 tgaatccatt cttaggatat cgtgcattgc gtatcagttt atctgaacaa ggcgacgaaa
     5041 tgttccgtac acaaatgcgt gcattgcttc gtgcatctgt tcatggtaac ttacgtatca
     5101 tgttccctat ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc tttgaagaag
     5161 aaaaacaaaa attgatcagt gaaggcaagg aagtatctga tacgatccaa gtcggcatca
     5221 tgatcgagat tccagcagca gcagtcttag cagacaagtt tgcaaaagaa gtagatttct
     5281 tctctgtagg tacgaacgat ttgattcaat acacaatggc agcagaccgt atgaacgaac
     5341 gcgtttctta cttgtaccaa ccatataacc catcaatttt acgtttgatc aaaaatgtaa
     5401 ttgatgcagc acacgctgaa ggaaaatggg caggtatgtg tggagaaatg gctggagatc
     5461 aaacagctgt tccattgcta gtaggtatgg gtctagatga gttctcaatg agtgcgacat
     5521 ctattcttaa aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg gctgaacttg
     5581 ctgatcgtgc attgaaagaa tgcgatacga tggaagaagt cgttgaactt gttcatgaat
     5641 atgtaaaata aacaacgtat caaaagctgg tgtggcttgt aaaagagcta cgccagtttt
     5701 tttgttcttt tttaagaaaa cgtttttgtc agtaagaata gataaaaata gggcttaagt
     5761 ttgagtgaca gtttgtaggc tatgttatac tatataagtg aagggggacg taaggtgaag
     5821 gtattattat attttgaaag tgaaaagatg cttgcaaaat ctggaatcgg gcgtgcgctt
     5881 gatcatcaaa aacgtgctct gacggaagtc ggtatctctt atacactaga tgaaaaagaa
     5941 gattatgaca tcctgcacat caatacatac ggaatcaata gtcataatat ggtcaataaa
     6001 gccagaagag aaggaaaaaa agttgtctat catgcgcatt ctacagagga agatttccga
     6061 aattccttta tcggatcgaa ccagctttct cctatcgtaa aaaaatatct ggtaggattg
     6121 taccaaaaag cggactatct gatcacgcca acaccttatt caaaacagtt gttggaaagc
     6181 tatagtatcc gtgtgccgat tcaagctatt tccaatggga tcgacttaga gaaatatcgc
     6241 cctgatccaa tgaaagaaca aaagttcaga gaatatttca aactgtctcc ggatcaaaaa
     6301 gtaatcattt gtgtcggttt gtttttcgaa cgtaaaggaa tcattgattt cgtagaaatt
     6361 gctaagaaga tgcccgaata cacatttatt tggtttggtc atgttcccat gtactcgatt
     6421 cctcggaaca tccggaaaat tgtaaaagaa gatcatcctg aaaatgtcct gtttcctggt
     6481 tacattcgtg gagagatcat agaaggtgcg tattcaggtg cagatctatt ctttttccct
     6541 tcttatgaag agacagaagg gatcgttgtt ttagaagctt tagcaagcaa acaaaacgta
     6601 cttgttcgag atattccagt gtataatggc tggttagaag atagcaagaa ctgctatatg
     6661 gggaagacaa acgaagaatt tatccgactg atccaacaga ttaccaatca agaactaccg
     6721 agtacgacac aagccggtta tcaaacagca aaagagcgaa gtatcaataa aatcggtgaa
     6781 gaacttaaaa atgtatacga aagtgtactg aatgaaaaag tctctagcaa attaaaaaaa
     6841 gtaaatcagg tcaaggacta gtttggaggc gaaaaacatg aaaatcggct tttttaccga
     6901 tacctacttt ccgcaagtca gcggagttgc tacatcgatc aaaacattaa aacaagaatt
     6961 agaaagacat gggcatgaag tctatatttt tacaacgacc gatcctaatg cggataagtt
     7021 ggaaaaagat gtgattcgaa tgccaagtgt accatttatt tctttcaagg atcgaagagt
     7081 tgttgttaga ggcatgtggt atgcttattt ggttgccaaa gaactagaac tagatcttat
     7141 tcatacccat acagaattcg gagcagggat acttgggaag atggtagcta agaagttgaa
     7201 aatacctctg atccatactt accatacgat gtatgaagac taccttcatt atatcggtaa
     7261 aggaaaagtg atcaggcaat cccatgtaaa atacttgtct agattatttg caaatcatac
     7321 gacaggtgta gtttgtccaa gtgaacgtgt tattgataca ttacgtagct atggtgtaat
     7381 agcaccgctt cgcgtgattc ctactggtat cgacattgaa aaattcaaac gttctgatat
     7441 cactcagcag gatatcagca aattaaggga gtcactaggt cttcaggaaa accatttgat
     7501 gattctttct ttaagcagga tttcttatga gaaaaatatc caagccttga ttaatgggtt
     7561 tcctcaggtc atctccgctt atcctaacgc cagattagtt atcgtaggaa aaggacctta
     7621 tgtagaagag ttagaggaat tgatctcaga actgcagtta gaagaatttg tgcagttcac
     7681 aggagaagtg gacaacaatg atgtagcact ttattataaa gcggcagatt attttgtcag
     7741 tgcttctact tcagaaacac aagggcttac ctatacagaa gcgatggcag ccggaacacc
     7801 ttgtgtcgtg gaaggaaatg cttatttgaa ccgattgttc gatcatcctt cattagggaa
     7861 aacttttgaa accgacgaag actttgcacc agcacttatt gattatattt ccagtgaagt
     7921 tcctaaagat ccaaaagttt tagaagctaa actttatgat atttcatcta cacatttcgg
     7981 cgaagctatg atcgaatttt atcaagacat ggttgtttat catgaagaag agatgcgaag
     8041 aaaagaagaa gaagactcga ttgaacgaat caaaataaaa ttaaattcat ttaaaagata
     8101 aaattcaaac attttttatg aaaaaatcct tgtttctagc gtatactagc atagaagcaa
     8161 ggatttttga tttttaggag gtcgaaaaaa tgaaacttgg ttttatcgga acaggcgtca
     8221 tgggaagtgc tgtcgcacgc catctactag aagcaggaca cgaagtagct gtctataatc
     8281 ggacaaaagc aaaagctgat cctttagtaa cagaaggagc tatctgggca gatacaccaa
     8341 aagcggtagc agaacaaagt aatattctct ttacaatggt aggttacccc aaagatgtag
     8401 aagaaatcta ctatggacag tcggggattt tttctgcaga tatctcagga catattttag
     8461 ttgacttgac tacaagtaca ccaagcctgg cagaaaaaat tgcaaaaaca gcaaaagaaa
     8521 aaggcgcaga tgcgttagat gcaccggtat ccggtggtga cttaggagca aagaatggta
     8581 cactgacgat catggtcggt ggagaagaag ccgtttatga tcaagtactg cctttattca
     8641 aagaatttgg tacgacattt acattgcatg gttctgctgg gaaaggtcag catacaaaaa
     8701 tggctaatca gatcatgatt gcaggtacga tgacaggaat gacggaaatg cttgtctatg
     8761 cacaaaaaaa cggacttgat ttaaagaaag tgattgagac gctttcaggt ggaagtgcag
     8821 ctaactggtc tttaagcaat tattctcctc gtattttaaa agaagattac acacctggtt
     8881 tttttgtcaa acattttatc aaagatttaa agattgcatt agaagaagca gaaaaaatgg
     8941 accttgtatt gccagcaact acccaagcat tgaaactata tgaagagttg gcagataaag
     9001 gttttgagaa tgacggaacc caagctttga tcaaactttg gtggcctgaa ggaaaaatac
     9061 cagaaaaaaa atcataaaaa acgagttttt ttagagaaaa tcaatgcaag taggctatgc
     9121 ttttgataca atataacaga aataagaaaa ggaggacctc cgatgacaca ttcacaatta
     9181 gtcgcaatca taaaacgact ggaggctatg gtcgagtcag cagataatga actgcaagtt
     9241 cgccgttttg aaaaagaagg cgtagagaag tgtacagtaa cttatgataa agcgaccgat
     9301 acatttgaat taactgaaac agatactcat cagcaatacg aatttgataa tatcgatatc
     9361 gtagcgatgg aaatctatga cttaatccaa taaattttag gataattgac atttgaagtt
     9421 gaagatgcct aaaagaagcc gagaagcaac taatttagtt gcttttcagc ttcttttttg
     9481 tttaacagtt caatttcaga ctgttctttt tctaattttt tgcggtaact gatcaagatt
     9541 tgtacaggat cgatttcttc ttttgacaat tgacgtttca aagataaatg agcttctttc
     9601 ttttcataat agaagtcagg agaaacgtca gcagaggcaa tcattgtttc tgcaagttga
     9661 ccataacaaa cgaggtagtg ctttttctga ctctcatctg ctgctaatgg catgctgaag
     9721 gtgagcgatg gtgttcttaa aaaatgcttt tgttcctgga tccgtctaat aaaataagta
     9781 actaattcga tatctttttg aagcataccg gctttttcga aattcaactg atagatgaaa
     9841 tgttgttgtc tttttttcaa gtaagttagt agctttttat tttggccttg aaacagccct
     9901 tggagttcct tttttttctg ttcaaaagtc atttcctgta ggtcaggaag ctgggctcgc
     9961 aaagctaaca aactaatttc agacaaccaa ggcatttgat aagtttcttc cattatacgc
    10021 aaaatagacg gcattttttt atagagacga aaaggcccgt atgttcgagc cgtaggagta
    10081 tctgttaata caaatccctt attggtaata tgtacataat tataattact gaaataattc
    10141 atttgtcggt tataaaaagg acggtaatgt tggatcaatt gacactcgag caataaagca
    10201 tctaattccg tgtctactac gacgaaatca aaatctgtta tatgatgaat catacgtaat
    10261 actttttttg aatgctgttt attctgatga aaatagctgc ttactctatt tttgagccgt
    10321 tttgctttac caacatagat gaccttacca tcacggtctt tcataagata gacacccggc
    10381 agcatgggga gagttttcat tttttctttt aacataggat ctccttttac gctttgaata
    10441 aaaagactat aacaaatttt gaaataatgg acaagtttta aggaattcta tagttctgtt
    10501 cacagaaaat actttgataa tcaaaatatg tgagatagat gatttttcta ataaaagaaa
    10561 ttataaatgc
//
LOCUS       DAZKCN010000087        10498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000087 DAZKCN010000000
VERSION     DAZKCN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.089"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8K3_002108"
     CDS             33..656
                     /locus_tag="ACG8K3_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145958.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8K3_002109"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8K3_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9145959.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8K3_002110"
     CDS             2152..3033
                     /locus_tag="ACG8K3_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9145960.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8K3_002111"
     CDS             3036..3806
                     /locus_tag="ACG8K3_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9145961.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8K3_002112"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8K3_002112"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9145962.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8K3_002113"
     CDS             5117..6280
                     /locus_tag="ACG8K3_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP9145963.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8K3_002114"
     CDS             6397..6807
                     /locus_tag="ACG8K3_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9145964.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8K3_002115"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8K3_002115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9145965.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8K3_002116"
     CDS             9380..9664
                     /locus_tag="ACG8K3_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145966.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8K3_002117"
     CDS             9824..10060
                     /locus_tag="ACG8K3_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9145967.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8K3_002118"
     CDS             10057..10455
                     /locus_tag="ACG8K3_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9145968.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3660 a         1766 c         2224 g         2848 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcatt
//
LOCUS       DAZKCN010000088        10496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000088 DAZKCN010000000
VERSION     DAZKCN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.090"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..460)
                     /locus_tag="ACG8K3_002119"
                     /pseudo
     CDS             complement(1..460)
                     /locus_tag="ACG8K3_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="ACG8K3_002120"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="ACG8K3_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9145969.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="ACG8K3_002121"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="ACG8K3_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9145970.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="ACG8K3_002122"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="ACG8K3_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9145971.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1964..2089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2240..2872
                     /locus_tag="ACG8K3_002123"
     CDS             2240..2872
                     /locus_tag="ACG8K3_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9145972.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="ACG8K3_002124"
     CDS             complement(2874..3779)
                     /locus_tag="ACG8K3_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP9145973.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4523)
                     /locus_tag="ACG8K3_002125"
     CDS             complement(3864..4523)
                     /locus_tag="ACG8K3_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP9145974.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="ACG8K3_002126"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="ACG8K3_002126"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP9145975.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="ACG8K3_002127"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="ACG8K3_002127"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP9145976.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="ACG8K3_002128"
     CDS             6766..7851
                     /locus_tag="ACG8K3_002128"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP9145977.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /gene="fni"
                     /locus_tag="ACG8K3_002129"
     CDS             7848..8912
                     /gene="fni"
                     /locus_tag="ACG8K3_002129"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9145978.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="ACG8K3_002130"
     CDS             complement(9161..9964)
                     /locus_tag="ACG8K3_002130"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9145979.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10307)
                     /locus_tag="ACG8K3_002131"
                     /pseudo
     CDS             complement(9984..10307)
                     /locus_tag="ACG8K3_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10340..10477)
                     /locus_tag="ACG8K3_002132"
                     /pseudo
     CDS             complement(10340..10477)
                     /locus_tag="ACG8K3_002132"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3106 a         2219 c         2008 g         3163 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaatccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DAZKCN010000089        10333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000089 DAZKCN010000000
VERSION     DAZKCN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.091"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..321
                     /locus_tag="ACG8K3_002133"
     CDS             172..321
                     /locus_tag="ACG8K3_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP9145980.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            332..1846
                     /gene="dltA"
                     /locus_tag="ACG8K3_002134"
     CDS             332..1846
                     /gene="dltA"
                     /locus_tag="ACG8K3_002134"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP9145981.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1843..3048
                     /gene="dltB"
                     /locus_tag="ACG8K3_002135"
     CDS             1843..3048
                     /gene="dltB"
                     /locus_tag="ACG8K3_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP9145982.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3093..3326
                     /gene="dltC"
                     /locus_tag="ACG8K3_002136"
     CDS             3093..3326
                     /gene="dltC"
                     /locus_tag="ACG8K3_002136"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP9145983.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3329..4597
                     /gene="dltD"
                     /locus_tag="ACG8K3_002137"
     CDS             3329..4597
                     /gene="dltD"
                     /locus_tag="ACG8K3_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP9145984.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4738..5820
                     /locus_tag="ACG8K3_002138"
     CDS             4738..5820
                     /locus_tag="ACG8K3_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP9145985.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5820..6623
                     /locus_tag="ACG8K3_002139"
     CDS             5820..6623
                     /locus_tag="ACG8K3_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9145986.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6693..7433
                     /locus_tag="ACG8K3_002140"
     CDS             6693..7433
                     /locus_tag="ACG8K3_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9145987.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7444..8448
                     /locus_tag="ACG8K3_002141"
     CDS             7444..8448
                     /locus_tag="ACG8K3_002141"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP9145988.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8493..>10333)
                     /locus_tag="ACG8K3_002142"
     CDS             complement(8493..>10333)
                     /locus_tag="ACG8K3_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145989.1"
                     /translation="FFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPAD
                     SNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIK
                     GNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAG
                     LPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLIL
                     LSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWY
                     GIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFY
                     VACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDK
                     DRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYV
                     EKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLA
                     QLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEE
                     LDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3328 a         1703 c         2088 g         3214 t
ORIGIN      
        1 tttaaaagta cgagaaatta taaagaaaaa agcagtgatt tgatgtgcta tttttataaa
       61 agctatgtga caaaactgtt agtaaaaaaa cggtttctgt aagctagaca catgtttctt
      121 tttgtcaaaa ttagttacac tttgtgtgag gttatatgag gagggaatcc aatgaacaaa
      181 tggcgttcta atccaaaatt gaattattgg ggaaaattca tcattcgaac aattttttat
      241 agtgcagtac tgctgttcct gatttatctg tatcactata aaaatattca aggaggaact
      301 tttatctata acgaatttta ggaggaagag tatggaaatc aaaacgatta ttgaagcaat
      361 cgatgattgg ggaataaatg aacctgatcg agttgcttat caggcggatg agacgcatac
      421 ttttggtgaa ttgaaacaag catcagacgc acttgcttat tatttagaaa aaaaggtaga
      481 aggcgatgga ccaattgtcg tttttggcaa tttggagttt gaaatgattg tctcatttct
      541 aggggttgtc aaatctggcc atgcctattt gccgattgaa gagcatacac ccaaggagcg
      601 tatcctatcg atctttcggg tggctaaacc atctatggtc atcagtatcg gggactggat
      661 agaagatatt ccttcggttc ctgtcataac aaaaaaagaa tttcaagaaa tcaagaagat
      721 accagtagga ttttatccca gaaatagcgt aaaaggtaca gaaaattttt atattatttt
      781 tacttctggt actactggag aaccaaaagg cgtacaaatc agtcatgata atctagtcag
      841 ctttgttcaa tggacgatgg aagactttgg gatcaagcct ggtatgcatt tcttagccca
      901 agcgccattt tcttttgatt tatcggtatt ttctatttat cctgcattag tttctggcgg
      961 aatgctgaag ccgttaaata aagcagttgt tcaagacttt cgtcaattat tcgcgacatt
     1021 gccagccttg aagttgaatg tgtgggtttc tactccatca tttatggata tttgcttgat
     1081 ggaacctaca ttcaatggag aaaatgtgcc ggaattggac atgtttttat tttgcggaga
     1141 agaattgacg aaaaaaactg cagaaagtct acttgatcga ttcccaaatg cacggatata
     1201 taatacttac ggtccaacag aagcaactgt agctatttca agtattcaga ttgaccagca
     1261 tgtactagat agttatgacc gactaccgat tggctacgta aaaaaagaca cgaaagtttc
     1321 tattgtccaa gaaggtaaac ctgtttcaaa aggagaaacg ggtgaaatca ttattgcagg
     1381 accaagcgta tcaaaaggtt acctgaacaa tcccgaaaag acagctgctg cgttcttttg
     1441 tcaaaatgac ttagcaagtt accatacagg ggatgcaggt agactagatg aatatgggct
     1501 tttattttat gaaggacgta tggatcagca ggtcaaatta catggatacc ggattgaact
     1561 cggggatatc gaacattatc tgctccaaga caaccgaatc aaacaagctg tcgtcgtacc
     1621 aaaataccaa ggaacaaaag tacagcagct ggttgctttt gtcgtattag aaaacaaaac
     1681 gaaacagccg gattttcagt tgactaaatc tatcaaagaa caattactag aagttgtaat
     1741 ggattatatg attcctcaga agttccatta tgtcgatctg ctgcctcaga cagttaatgg
     1801 aaaaatcgat cgaaaaaaat taacagctga ggtgaatcct gaatgatgtt acctcatatg
     1861 atcccttatg cagagccgtt ttattttttt cttttagcca ttgcgcttgt accgatcgtt
     1921 ctatctttat tgatatggga aaaaagatta ccagtttatc aatcagcagt gacacttttc
     1981 tttttatttg tcagctttgg aggtaattat tggcaacagg gagcggcgct gattgggtat
     2041 gttttgtggc agacgatact tgtatggggc tattttgttt atcggcaaag gaaaaatcag
     2101 acaggatggt tttttgccgc cgttatttta gcgataattc cactgttttt aacgaaagtg
     2161 actccttttt ttgaacatgg aaaagcatcg ttacttggtt ttttaggaat ctcttattta
     2221 acctttaaat ctgtacaaat gatcatggaa ataagagatg gcatgatcaa aacatataag
     2281 ccatttcgtt atatccagtt tctgctgttc tttccaacaa tctcttctgg accaatcgat
     2341 cgctatagga gatttgaaaa agatcagctc catccgcctg ttcgtgataa atatgttgat
     2401 cttttgggaa aaggcgtcca aaatattttt gtcggttttt tatataaatt cattatcggt
     2461 tattattttg gtcaagtact gcttcctgta gtgggacgca ttgccttaca gcatggtggt
     2521 atttcatggg cattagtagg ttatatgtac gtgtacagta tgtatttgtt ttttgatttt
     2581 gctggctata gcttatttgc agtcggtaca agctacatga tgggatatca gacaccaatc
     2641 aatttcaata aacctttttt atcatggaat atcaaagaat tctggaatcg ttggcatatg
     2701 acgctgtcct tttggttccg tgattatgta tatatgcgat tgatgttctt catgatgaag
     2761 aaaaaatttt ttaaaagcaa gatcgtaatg tcaaatatcg gttattttgc tttgtttctt
     2821 ctaatgggta tatggcatgg attgacgtgg tactacatcg tatatggact gtatcatgct
     2881 tgtctgatct gtctgacaga tgcatggatc cgatttaaga agaagcataa agagcagttg
     2941 ccttctaaca aatttacgca tggcttagcc gtatttttga catttcaagc agtatgtttt
     3001 agcttcctga ttttttcagg atttttagat aaactttttt ttagctaacg aatagtgaca
     3061 tattaattta tataacaaag gagaaaagga aaatggaaga acaaatttta tcaatcttag
     3121 aagaaataac aggaacagat gaagtaagaa aaaatcgtga tattgatcta tttgatgaag
     3181 ggttgcttga ttctttagca tctgttcaat tattagtcga gctagacgga gagctggata
     3241 tccaagtccc tgtatctgaa ttcgagcgag aggattggag cacaccgaat aaaatcattc
     3301 aacaagcaac tgcattgaag ggttgagtat gaaaaagaaa ctttttgcta ttttcggccc
     3361 ggttgtttta gctgttggct tactgtttgt gttttttacc tcctcctatc aagtagatat
     3421 ctctaatcca gaaacgatta gacgtgcctc ttcttccatg tcagtgaacg tgttgaaagg
     3481 agatgcgata aaaaattatg cattatctga aaaacaatat attccttttt ttggctcaag
     3541 tgaattaagc agaatcagtc catttcatcc gtctgttctg gcagaaaaat accagcgaaa
     3601 ctatcgtcca tttttgctgg gagcacccgg gacacaatcg ttaagccaat atatgatgat
     3661 gcgttctgca ggagatgcta tgaaaaacaa gaaagtcgtc tttattattt ctcctcagtg
     3721 gttcgttaaa aatggtgtaa aaactgatta ctttaatacg tattattctg aacttcaaac
     3781 atacgactgg ttgttttcaa tgaagaaagt aacgcctgct gatcgctatt tagctagaag
     3841 attattaact ttctcaaaag taaaagagaa tgatacgttg acggcgattc tgcaaacgat
     3901 caaaaaaggg aagctacctt tacctgaatc tttgaatcag ctgcgcagcc aatggaacat
     3961 gctgaagaga gaggatgaag tattcagcaa tattgggttg aaagatcgac agcaaaaaat
     4021 cgatcatgag tcaaaacgat taccaaaaca ataccaagaa acagaattat ccattttagc
     4081 aaatcaaatt ggagaacgag aaacgacgaa taatccattc gggttgaaaa acgattttta
     4141 tacgcatcgt atccgagccc acgagccaga gctcaaacag tcacaaaaaa attgggatta
     4201 tcgtttttcg ccagaatttt ctgattttca gctggtcttg gatcaattag caaaaaacca
     4261 taacgaagta ttgttcatca tcccaccggt taacgagaaa tggagcgatt ataccggact
     4321 atctcaagaa atgttacaag gatttgccaa aaagatcaaa tttcaattaa atagccaagg
     4381 attcaatcga atagctgatt ttgtcaatca agcgggtacc aactatttta tggaagacac
     4441 gatccatcta ggctggaaag gctggttggc tgcagatcaa cagatcagac cgtttttaga
     4501 agaaaatcat attactgcta gtaagtatca tcttgacgat gcttttttta gcaaatcttg
     4561 gcaacatcaa ataccagata aactacaatt aaaataaaga aaacaggcga gtgttgctcg
     4621 cttgttttct ttatttttga atattctgaa taaaagataa aaataccaag tttgcctgaa
     4681 caaaaatcat aagcaatggt ataatcacaa tatgaatata gaaatggggg aaaacatttg
     4741 gatacaaaag aagaacagct acttattgat ttaacttctg caaaaggagt acctggaaat
     4801 gaagaagagg taagagaagt attcagagaa tatgccaaac cattcgctga tgatattttt
     4861 tatgacggtt taggcagtgt gattgccaaa catggtgctg gcggaggccc caaggtcttt
     4921 atttctggac atatggacga agttggtttt atggtaacaa aaataaccga aaaaggattc
     4981 ttggaattcc aaactcttgg aggttggtgg gggcaagtca tgcttgctca gcaagttgaa
     5041 atcaaaacac gtgaagggaa aatcgtccac ggtgtgattg gttccaaacc accacatgtt
     5101 ttaacacctc aagtaagaaa taaaccttat gaaataaagg atatgttcat tgatattggc
     5161 gcatcaagcc aagaagaagc caaagagtgg ggcatacgtc ctggagatat ggtcactcca
     5221 tatattgaat acaaacgaat gaatggcagt aaatatttac tggcaaaagc atgggataat
     5281 cgtatcggga cagctgtttc tctgcgagta ttagagaatc tatctaaaga agcacaccca
     5341 aatgttttat tcgcaggaag cgatgtgcaa gaagaagtag gcttgcgagg tgctcgaaca
     5401 agtacacacc ttgtaaatcc cgatattgct tttgctttag atactggtac agcaggagac
     5461 actccaggaa tgacaccaaa agaagcagat tcgattttag gaaaaggccc acagattttg
     5521 atttttgatg catctatgat cccgcataaa aaattattga attttgttat tgatgtagca
     5581 gaggaaatgg atattccatt ccaatataca gtgatttctg gcggaggaac tgatgcagga
     5641 caaatgcatt tgacaagaga tggtgttccg tctgtagcca ttaccgttcc tgttcgttat
     5701 ctgcattccc atacatcagt gattcatgaa gatgattatt tgaatacagt gaaactagtc
     5761 acagaattag tgaaacggct tgatagtgaa acagttgaaa aaattcggag ttatgaataa
     5821 tgaataatag aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc
     5881 aatggggaat caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga
     5941 acttttttga cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt
     6001 ttctagtcga taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag
     6061 tagttgtacg gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt
     6121 attttggttc gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca
     6181 gtttaatctt tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt
     6241 tcagttcaaa tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg
     6301 ttttatcgat gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt
     6361 tattccaatt tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt
     6421 tgaaatatgg ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt
     6481 cttttatcgg ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca
     6541 ttgtttatgt gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag
     6601 gtccagacgc ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg
     6661 tggtaggata aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag
     6721 gagcgtatcc ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca
     6781 atttgttgat cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac
     6841 ttgatctaga tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg
     6901 tattacagta ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt
     6961 atggacacac aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag
     7021 gacatcctta ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg
     7081 aaacaatcca tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag
     7141 tattcgtatt tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg
     7201 ctgatttgtt tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt
     7261 tcacttcaaa agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta
     7321 cacacctacc tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag
     7381 gcaaagggat tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag
     7441 aacatgattt ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa
     7501 tatttgctgc aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct
     7561 tcaatcatca tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa
     7621 gaaaatggca ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt
     7681 ggaaacctga actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg
     7741 aaagtaacac agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg
     7801 aaaggccgaa atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct
     7861 accaacggtc ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta
     7921 gtcaatgcat taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt
     7981 tctcgtgtaa cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa
     8041 gaatttcgtc aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa
     8101 acgtacgaat tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat
     8161 cgaaattggg attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt
     8221 ttttctatcg gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca
     8281 aaaatctttg gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt
     8341 ggacagaaat atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa
     8401 tattttggtg cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata
     8461 gcggaatcca catatatgga ttccgctatt ttttatacag gattcgggta atttcgagag
     8521 agttgaacag tgatccgatc aatgaaagta tctttttcct gcgttagtgt tcctgtgtaa
     8581 cgatccgcta cgtctttcat catcattaag tcaaaacgtt cctgatcatc taattcttct
     8641 gtgatgcctc gactaaatag gtttgtatcg taatgtgcac tatgttcgca acaaatgacg
     8701 aaatgttcct cttgttcgtg tatcgacaaa taaatttttc gatacagtgg attttcgatg
     8761 gcatgagttt ctctaaagga atgctctaaa gcgtggatca aaagttgagc cagatcgtca
     8821 tcgttgatag gaagttcttc gggaagatct gcttggaagg ttatctgaat attccgtttg
     8881 gcagctaact tctggtaacg cgctaagata agatttgtca cctggtggtt gcaaaaagaa
     8941 cacagcattt cgaaattttt tgcttcttct aatatctttt ccacatagtt tttagctttt
     9001 gtttggtccg tatgcattaa atcttgcaga ttctccatct cttggacaaa ttccaggcga
     9061 agtgtattta cttcttggat atgctgtcga agctgctcat aatgttgctc caacaaatca
     9121 tttttgattt ttaagaaatc tacttgtctc cgatctttgt cggtatttaa gatgtgatcg
     9181 attagattgt agctgataat aacgattaga atgataatat ataaaatcgt agaccaattg
     9241 atcgttgttt gatagatacc taatgccgtt tggatgtaaa tcaagcaatg gataatggct
     9301 accaagacga tccaaggtgt acaagctaca taaaaacggt tattcttata tgcttcgcca
     9361 atgcatgcaa gtgaagtgac taaagtgctt ataacactag caatattagc aatcggcatt
     9421 aattcaggaa gttctgcagg agtaaaagtt gcataaagca gaacgccgat aaaaatcaaa
     9481 agttggaacg tacaaaaaat tccgtaccat tttttatagt aaaacattcg gctccaatag
     9541 tagcagatga tgaaagctga cgttaagata ataaataaat gagataaaaa agaatgaacg
     9601 aatggttcaa aaagaatacc acttaaaata tcttctgata cagcttcaaa tcctaatgcc
     9661 aaagcaaagc aacttaagag aataagtgag tattcaagac gtttttgttt atacatcaag
     9721 tagatcgtat agaaaaaggt ccctatagcg ataaaaacag cgattatcat cgtcaatact
     9781 tgcggcactg attgtgaata aataaaacta atcaatggtc cagttgttcc tagataaact
     9841 ttcggtggca gtccagcata gttctgataa ggagatgaga ctgtaagggt caattttttt
     9901 ccgatataat ttgctggtaa atctatcaca gccaatgaga gtccgggatt gctttcttcc
     9961 tgataatctt tccgttggta taaaatcttt tcgcctagat atacagtaat ccattgatgg
    10021 tttcctttga ttagtaaatt tgcttcgtcc aacggttttt caagtgtcgt ttccataatg
    10081 aatgtttcgt ctttatccac ggtaggaatc gtacgagtgt acttgctgtc aaacttaaaa
    10141 tcaggatcat cttttgtata gaaaacccac gaatctttta tttctttcat ttgattgctg
    10201 tccgcaggag aaaaaataaa ataaaagaaa taagcaattc ctgttaaaaa taacaatgat
    10261 aatataatga ttatgctcca ctttatacgt aaataaaaag attctttttt attatatatg
    10321 ttattaaaaa aag
//
LOCUS       DAZKCN010000090        10251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000090 DAZKCN010000000
VERSION     DAZKCN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.092"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACG8K3_002143"
     CDS             185..1183
                     /locus_tag="ACG8K3_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9145990.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACG8K3_002144"
     CDS             1295..1804
                     /locus_tag="ACG8K3_002144"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9145991.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACG8K3_002145"
     CDS             complement(1834..2493)
                     /locus_tag="ACG8K3_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP9145992.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACG8K3_002146"
     CDS             complement(2495..3259)
                     /locus_tag="ACG8K3_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP9145993.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACG8K3_002147"
     CDS             complement(3267..3944)
                     /locus_tag="ACG8K3_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP9145994.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACG8K3_002148"
     CDS             complement(3981..5366)
                     /locus_tag="ACG8K3_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP9145995.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACG8K3_002149"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8K3_002149"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8K3_002150"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACG8K3_002150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9145996.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACG8K3_002151"
     CDS             6305..7162
                     /locus_tag="ACG8K3_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9145997.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACG8K3_002152"
     CDS             complement(7243..7578)
                     /locus_tag="ACG8K3_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9145998.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACG8K3_002153"
     CDS             complement(7645..9015)
                     /locus_tag="ACG8K3_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP9145999.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACG8K3_002154"
     CDS             complement(9093..9629)
                     /locus_tag="ACG8K3_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9146000.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..>10251
                     /locus_tag="ACG8K3_002155"
     CDS             9938..>10251
                     /locus_tag="ACG8K3_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146001.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYP"
BASE COUNT         3092 a         2082 c         1684 g         3393 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag taaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc c
//
LOCUS       DAZKCN010000091         9964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000091 DAZKCN010000000
VERSION     DAZKCN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.093"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8K3_002156"
     tRNA            8..81
                     /locus_tag="ACG8K3_002156"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG8K3_002157"
     tRNA            101..173
                     /locus_tag="ACG8K3_002157"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG8K3_002158"
     tRNA            201..274
                     /locus_tag="ACG8K3_002158"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG8K3_002159"
     tRNA            288..361
                     /locus_tag="ACG8K3_002159"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG8K3_002160"
     tRNA            385..474
                     /locus_tag="ACG8K3_002160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG8K3_002161"
     tRNA            487..560
                     /locus_tag="ACG8K3_002161"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG8K3_002162"
     tRNA            579..654
                     /locus_tag="ACG8K3_002162"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG8K3_002163"
     tRNA            656..726
                     /locus_tag="ACG8K3_002163"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACG8K3_002164"
     tRNA            740..813
                     /locus_tag="ACG8K3_002164"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACG8K3_002165"
     tRNA            838..925
                     /locus_tag="ACG8K3_002165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACG8K3_002166"
     tRNA            947..1018
                     /locus_tag="ACG8K3_002166"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACG8K3_002167"
     tRNA            1041..1130
                     /locus_tag="ACG8K3_002167"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACG8K3_002168"
     tRNA            1143..1216
                     /locus_tag="ACG8K3_002168"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACG8K3_002169"
     tRNA            1235..1307
                     /locus_tag="ACG8K3_002169"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACG8K3_002170"
     tRNA            1311..1383
                     /locus_tag="ACG8K3_002170"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACG8K3_002171"
     tRNA            1388..1458
                     /locus_tag="ACG8K3_002171"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACG8K3_002172"
     tRNA            1471..1544
                     /locus_tag="ACG8K3_002172"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACG8K3_002173"
     tRNA            1556..1627
                     /locus_tag="ACG8K3_002173"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACG8K3_002174"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACG8K3_002174"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9146002.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACG8K3_002175"
     CDS             3216..4688
                     /locus_tag="ACG8K3_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP9146003.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACG8K3_002176"
     CDS             4771..5559
                     /locus_tag="ACG8K3_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9146004.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACG8K3_002177"
     CDS             complement(5626..6294)
                     /locus_tag="ACG8K3_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP9146005.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACG8K3_002178"
     CDS             6409..8121
                     /locus_tag="ACG8K3_002178"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146006.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="ACG8K3_002179"
     CDS             8124..9896
                     /locus_tag="ACG8K3_002179"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146007.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DAZKCN010000092         9924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000092 DAZKCN010000000
VERSION     DAZKCN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.095"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..96
                     /locus_tag="ACG8K3_002180"
     CDS             <1..96
                     /locus_tag="ACG8K3_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFP9146008.1"
                     /translation="LVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
     gene            902..1075
                     /locus_tag="ACG8K3_002181"
     CDS             902..1075
                     /locus_tag="ACG8K3_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146009.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKQTDK"
     gene            1479..2633
                     /locus_tag="ACG8K3_002182"
     CDS             1479..2633
                     /locus_tag="ACG8K3_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP9146010.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2606..3049
                     /locus_tag="ACG8K3_002183"
     CDS             2606..3049
                     /locus_tag="ACG8K3_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146011.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3351..3731
                     /locus_tag="ACG8K3_002184"
     CDS             3351..3731
                     /locus_tag="ACG8K3_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9146012.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3713..4627
                     /locus_tag="ACG8K3_002185"
     CDS             3713..4627
                     /locus_tag="ACG8K3_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9146013.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4587..4865
                     /locus_tag="ACG8K3_002186"
     CDS             4587..4865
                     /locus_tag="ACG8K3_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146014.1"
                     /translation="MKEEQTTNKNYRSKSLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            4867..5556
                     /locus_tag="ACG8K3_002187"
     CDS             4867..5556
                     /locus_tag="ACG8K3_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146015.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5676..5762)
                     /locus_tag="ACG8K3_002188"
     CDS             complement(5676..5762)
                     /locus_tag="ACG8K3_002188"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP9146016.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            6408..7340
                     /locus_tag="ACG8K3_002189"
     CDS             6408..7340
                     /locus_tag="ACG8K3_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9146017.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            7357..7827
                     /locus_tag="ACG8K3_002190"
     CDS             7357..7827
                     /locus_tag="ACG8K3_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9146018.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7911..8363
                     /locus_tag="ACG8K3_002191"
     CDS             7911..8363
                     /locus_tag="ACG8K3_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9146019.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFS
                     HSIATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
     gene            8490..9671
                     /locus_tag="ACG8K3_002192"
     CDS             8490..9671
                     /locus_tag="ACG8K3_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP9146020.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            9761..>9924
                     /locus_tag="ACG8K3_002193"
     CDS             9761..>9924
                     /locus_tag="ACG8K3_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFP9146021.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPN"
BASE COUNT         3682 a         1575 c         1635 g         3032 t
ORIGIN      
        1 ttagtaaata ttagaggtaa acaaactatg cgtacttgtt accttatgag ttggaatgct
       61 agtagtagaa cagccaaata ttattactac atttagtaaa aacaaaacat tctttctata
      121 caataaaata gcgtatagag aggatgtttt taatttgaaa aggagtggtt atctttatga
      181 aaaatagatc atttgctata ttagtattat ccgtaatttt attagatata ttgttaataa
      241 cattagcttt aaaaacttta aatttagtaa caagttttgt cattatacca gtaattatag
      301 gaatgtctat ttatttcatc aaaaaaaata aacaattttt ttgaatgaaa ttacgggcga
      361 aaataatcac tcaaaattat taataaaaac gaatcaacta ctttttctga agaaacatac
      421 aaccgtaaac aacaattgtt gtttacggtt gtaaattatc aaactatatg gtgacttgtt
      481 gcttaaaata ggttgaaatt tcgtttattt tttttgagaa attttaccca cgtactaccc
      541 atgtacctat ccacgtatta cccatgtacc cccccatgac aaatgggcgg aggggcttgc
      601 ggcgcttggt attaaaggca ttatccgcct cccaggtttc tgaccatttt ctctcataag
      661 tggggttttt accgcgttga gagaaaatgg tcagaaacct aaacgatcgg caaagaaaac
      721 ataccaagcg ccacaagcaa aaaaagttag agtaacttcc agaaaagaaa aatttttaga
      781 acaacttttg aaaatttgat tttgatttat atccgttcaa tttaaacatt tgataaactg
      841 aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg aggtataacc
      901 tatgttaaaa aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag
      961 ccccatgcta ttatccgtaa ttggaaaaaa caattccaac agagaactta aattaaattc
     1021 aaattattac aacagtccta acgaagaatt gtttgtaaaa caaacggata aataataaaa
     1081 aagactgctc tttcgagcag ccagttaaaa aataatgagt gtttattctt gaaaaagcac
     1141 gcgcttatga agcgctacca aaaatttacc atattttaca aaaaataaca agtcttaaaa
     1201 aaatgacaga taaaaatgca caaagaatta gatatagata accgcgaaaa atcaagcttt
     1261 agaaagttgc ttttagctag accaattaaa aatcacaaag tgagcgtctc tatccattgg
     1321 tagaggggct acttggcata ctgtacatta tgttaactaa cgctataaat aacgattatg
     1381 ttcccaaaac aatattttat gaactataac aaaaatttgt tatcgcatat tgtatatttt
     1441 aattataggt gtataccact taaatttaac acaaaggcgt gattattttg aaacccaaaa
     1501 agaaaattta tctaggtctc ctcttttcct tgatgattgg aatttttagt ataggaatta
     1561 caaataccta tgcttcagaa aattctaatt tcaatatcaa ctcccaacca attgaagaac
     1621 aagcaattaa ttttgcgaaa actgattttg aacactccat aacttcaatt cttttaatgg
     1681 aaaataacac cgaaactgat attaactata ctcttggttc gccatttaaa atgctcgatg
     1741 gagaaaatac ggcaaatagt tctattttct ttccagtatt ttcagacgct gataatacga
     1801 ttaaatatat ttataccgtc aaaaaaaata caaatggaga atattcagga agtatttcac
     1861 aatttttagc aaaagaactt caaaatctag tagatagtaa tataacagat acaaccttat
     1921 tttttcaaga taatagcatt tattattccg ataatggaca aattaaacta ctttggaaat
     1981 cacctttaag tacagaaaaa aatggtgttt cttcctttac agaaaaaaat gatcttatat
     2041 ccgtaaatat aaaaaataaa attaaaaatt tctctaaaaa acaaaatttt cctgcgatta
     2101 aacgtgtaaa aagtgtctcc tccggagata actttattat gattaattgg cgtatagcag
     2161 aaacccaaac aagttttccg tggtgtgctg cttacgtaga agctgctatc ttatgtaaca
     2221 aaacagatgt acatgctact tcagctagag ccattatgca ttatacctat cctaatttaa
     2281 acaacagtca aattgaatca aaaagtctta atcaagatca aataataaaa tatgcaaatt
     2341 actgtggtgt ttatcctaaa aaagtagcac gaatactaaa cctttcagag gtccaaacac
     2401 aactaagaaa aggaaatgcc atttatatgg gaactgctgg ttatggaaat tatagtaaca
     2461 gccgtcatgc acttgcatta tttgggtggg tcaatcaagg tggttatcaa acttactatg
     2521 tgtggaatcc ttggtataac tacccaactg taataagtgc aaatgcagga ccagttacaa
     2581 ttcctgtacc tggaggtggt tttacttggg acaataccat tacaaattgg taaataaacc
     2641 cattgcttta gttatgggtc tagtgttagt tcttttctta actagttgtt caaaaaaaca
     2701 agaaatacaa aagactgatc tgattccttt tctaggaact tggcaaacta aagatcattc
     2761 agaacgtcta gtaatcacta atttaaatac ttcaaaaaat atagaacaat ataccatctt
     2821 agtaaaagag aacaatcata aaattcaaat cattgataaa aatttaaata taaatgaaca
     2881 agttttcatt tacaaagcaa caaataattt actaacactg cgctctaata aaaaacaatt
     2941 agattacact tttagtttgg attctccaaa aaagctcgta tttattttga caacgaatga
     3001 tgggacagaa gggacagcta aaccaattga attttttaaa gctgattgat gaatagttgt
     3061 ttgatattac aaaacaataa tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg
     3121 gctagcattt gctagccttt tttgtattgt ggagcgtggg ttttgagggg gattcaaggg
     3181 ggagcttgcg cccctttgaa aagcggttat ttgcaccaaa tacgtaggta tttggctagc
     3241 aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca
     3301 atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac
     3361 aaaatcaaaa tttggctagc gatcattcta aaaaatatac ctatcgatcc gagccaaaac
     3421 aaatcagttt tcgagtgagc gaatctgaat ttgcaaagct gaagcagtca gctgaagctt
     3481 tgcaaatgag tgtgccggct ttcgtaaaag ccaaggcaca aaacgcgcgt ctggtgacac
     3541 cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga
     3601 atatcaatca aattgctaag tggtgtaata cacaccaaca cgacgttgct cctggagacg
     3661 cccaacgctt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac
     3721 agttaaagta agccgaacca cttcttgtag tcgtgcaatt aattacgctg aaccacgagc
     3781 aaccattaaa acaggaataa actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat
     3841 tcgcatgctg tatggaaaag acgaccgtgt acaggcacac ttattgattc aatcttttcg
     3901 accaggagaa attaccgctg aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat
     3961 agcccccgat caccaaatag ctatttacac acatacagac aaagaccata ttcataatca
     4021 cattgtcatt aactccgtta atctggagac tggaaaaaaa tttcaagctc atggtcaagc
     4081 atttctaaac aaatgttatg acctaaatga cgagatttgt cttgcacata acttaccaat
     4141 cactgagcga ggaaagaaac cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa
     4201 aaatgaacct gtttggaaag acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa
     4261 aaaaaccaga acctatgacc aattttgtaa ttccctcaaa acattgggga ttcattgctt
     4321 caatcgcggg aaaaatttca cttacgaatt gatccacaaa aagaaaaaag tacgatccaa
     4381 cagactagga aaggactacg aaaaggagac gattttacat gagctggaca gacgagaacg
     4441 aacctataac caacagcgaa ccaacgagct acgaagagct cgagctagca ttcgcgaaac
     4501 tgcagaagaa gtacgaagag aacaacgaac tcttacgcct gaacaaccgg aaattagacc
     4561 ggctcgaaga aaccatgaac aacacgttga aagaggaaca gaccaccaac aagaattatc
     4621 gctctaaatc gttaaaagac atagaaaacg aaataaagaa acaacgtgct tttctcgttc
     4681 cttatacgca agaaaggata caaactacga ttgatttaca agttaaaaaa ctccatcaag
     4741 agctattaga ccagcgaaat aaggaacaga aaaaggtgct tattcaacga tcattggatt
     4801 ttcttgcccc cttatgttgc ttagtcctga cactgttcgc attttggtta gtatggggag
     4861 gctaaaatgg cattttattt accctatcta ctcatttttg ttagtatttc aggaagtatt
     4921 tggctcatct ataaaatttt tcaaacaaga tattcattaa aaggctctaa aattcgattt
     4981 aagcgatttt tctttcttgg ctgtatattt tccttgataa tcgtttcaag cggcttattc
     5041 ggcgttttag agggaaataa gcgcgtttct agaagtattt tattaggaaa tgtcactcaa
     5101 aagtatgaat cagcgcgtga taaaaagaaa aaagagcaag tattagcaca aaaaatagaa
     5161 aaatttaccg cttgttatga ggacatgaat gatatttttg tgaagcaaga aaagcgtttg
     5221 acggataaaa atatggaaac actcactcga ctttatcgga atttgccaga agaacagcaa
     5281 aaagagtacc aagaaaaata tgaacaagta aaaaaagacg tgcaatatgt gaaagatacg
     5341 aaagtggaag aagcttgtag tgatttattt ggagatacaa ttcctttttc aactagcgaa
     5401 caagaacgaa aagaacgaca acaatcagtc acatatgaac gctatgaaat cttgcttcaa
     5461 caagcaatta acatacaaaa tctgactaaa aaagaaacag ctttaaatta tttaaaaagc
     5521 gtcaaggaat ggttagatca gcaacagcaa aattgaattt gattgaaatt catgttaaac
     5581 taaacatgca aatgttcaaa cattagatac acgaaagccc cagagaggtc ggattctctg
     5641 gggctttttt taggttgatg tcgcctttac tgattttact tcttgtttcg gttattcaac
     5701 caataactga atacagagat acaaactcct acaagaaggg ggcaaataaa cagttcaaac
     5761 atcagataca cctccttcca gtcgcaagac tgtcggatag gcgacaagtt attataccct
     5821 acaaaattaa atctgtgtgg caattttaag tatctcttat ttccgggttg ttagggcttg
     5881 ccctgaccgc ctgtagggcg ctttaaatgc cagataaagg tttttatact aagactgtct
     5941 taaaacggct tagacaagct cttagagcct caaccctcct ttttgaacaa tggttttagc
     6001 aaaggagctg gaggcgactg cgcaggtata atgtgagagc gtagcgatca cacctttttg
     6061 gcgaccgtag ggagcgaggt tttgaattag cagtttagaa aagttcgcga acctgtttaa
     6121 agaaaaaata attgtttttt taggtgggaa attttgtctc cctcttttat aatctttttt
     6181 taaacctctt tttaaacctc tttaccgtcg gctgtgtcct tactccccca agggatacaa
     6241 aattttatag gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga
     6301 aattatctgt ttataggcat gaaattatct gttttaggta ttgtttttag acataatatg
     6361 tcctaaaatg atgtcaataa aatcatttag actggagtaa caactaaatg gcaaatgaaa
     6421 tcgtaaagta tgaaccagaa ttaaatacca ttcctttaag aaaatttacc ccaatagaaa
     6481 tgaacttgtt tttttctatt gtctctagaa tgcgcgataa aggtaatcaa aaaatacgct
     6541 tttatttcag ccaacttaaa gatttaagtg cgtataagca gactgcagga gaagtcttta
     6601 taaaagattt ggcaagaaca tacgatcatt tgatggattt acgtttcgga agaaaaagca
     6661 atggtggatt aacaatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag
     6721 tcgataaccc ttatgtagat ataaaagtcc acgaaaaagc tttgccttta cttaataatc
     6781 tagaaagctg ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta
     6841 aaacaatgtt tagattgcta aaacaatacc gaactcaagg atgggctgaa ttttctaagg
     6901 aagatttttt agagctatta gatattccta aaagctatag acaaacaaat ataaatcagt
     6961 ttgtactcaa accaataaaa gaagaactaa ctccactatt taaaggatta actattcgta
     7021 aaaaatatgg taaaggtcga ggtaaacctg ttattggtta tcgttttact tggaaagctg
     7081 aaatcaacca tgctgacgat ttttcaaaag ggaagcaaga agatttacgt ataaaattat
     7141 ttaatattga acacaatgga gaattaacac aaaaagaaaa atggcgtgct aaagatcgaa
     7201 tactaaatct tccgttaggt actcatgaag ctgattttaa aaaacaacaa caaaccgaaa
     7261 aagaagaagc ggaaaaacaa tcaatctcaa atgagcttaa acaagattta ttagaaaact
     7321 tacaaaactt attcgattga agcataagga gtatttatga tagataaaac gatcaaagaa
     7381 ctcgcggaag aatttcatgt ttcaaaacaa gcaattagga aaaggctatc agctgatttt
     7441 agagaaaact atgtgcaaac tgttactagg aacggagtga aaaccttagt tgtcagctac
     7501 aaaggatatt tgcttttaaa acaacatttt ttaggtagta acaccactgg taaccaagta
     7561 gaaaccgttg ctagtaacga tgaaaactcg attattgttt ttttacatga acagctccaa
     7621 gaaaaagata ctcaaataaa acaaatgcaa aagttactcg accagcaaca acaactcacc
     7681 ttgcaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa
     7741 gaacctgaag aagaggaatt aacttctgtc aataaagaaa acaacaatgc aataacaaca
     7801 gaaaaaaagt ggtggaaatt ttggtaacaa ttaccaactt tccataaaga agaaatcaat
     7861 ttctatagac tctatctttc taagaataaa aaatatgttt aggtgatcaa atgactataa
     7921 gtaaagtaat caaacaaaaa agaatagaaa aacaactaac tcaagaagat ttagctgaaa
     7981 tgcttttagt atctaaaaaa accatttcta attgggaaaa tggtcgaaca ataccagata
     8041 cagaaaattt aattcagttt gctaagttat ttgatttatc actagacaat attttgttag
     8101 gagaagaaaa aattatggta aaaaatcata caaaaacact aatactatta tttcattttg
     8161 taattttttt aagtttagtt ttcaccttaa ttcagtccgg attcttttat tttagccatt
     8221 caattgccac ttggttatta atattttttc taagtttact attaataatt aatactttaa
     8281 ttattatctt taataaacat agaaacttat ttcttcacag catatttcca attgttttat
     8341 taatttttct atactttata taaaaagata gaactattta gtaagataaa aaagtagcta
     8401 attgtaattt tcgtctaaaa ctcaaaacta gtaaaaataa ctttattgtg ttatttttat
     8461 ttataaatta tgaaaaagga cctttcgaaa tgaaaaataa tagaatctac aaaaatcagt
     8521 tgaattatgg gcttttattg ccagtttttc tgctatgtat cattggatta ctttctttat
     8581 atgttgcttt atatcacgac tcttacacta attcaaaaat cacaaaagaa ttaattaaac
     8641 aagtattgtg gtatagtatt gggttcactt cagcaataac tatttatttt tttaatccta
     8701 aaattttgtg gaaattaact cctatatttt acggattatg catcataatg ttattacttc
     8761 tcttgaaatt ctataatcca caattagcag tcttaactgg agcaaaaaat tggttttata
     8821 ttgctggttt ttcgtttcaa cctactgagt tagtcaaaat aggatacacc cttatgctag
     8881 ctaaaataac cgtcaattat cagcaaaaat ctattactag taatacgaaa gatgattgga
     8941 tttatttact taaaactttt attgctactc ttccaatagg aatattatta ttttttcaga
     9001 acgatctagg aacaatgtta gtcttcattg ttatttatat ttttattttg tttacgtcta
     9061 acataaatat aaaaataatt gccccaataa tcgccattgg tgttgtttta tccttaatat
     9121 ttttatattt ggttatatca gatacaggaa gagatttctt attaaaaata ggatttcacg
     9181 actatcagtt taaaagaata gactcatggt tgaatccatt tttagatcca aatggatcta
     9241 gctatcagtt agcaaatagt ttaattgcta ttggctctgg cggactttta ggtactggtt
     9301 ttaatgtatc aaacattcac gttcctgtta gagaatccga tatgatattt tctgtaattg
     9361 gagaaaattt tggctttata ggaagttgtc ttgtaatatt cctatacttt tatttaattt
     9421 atcaaatgta taaaacatgt tttaaaagca ataacttatt tttcacctat atcggtatag
     9481 gcattgttgc aatgattttt tttcatgttt ttgaaaatat aggtgcaagt atcggattac
     9541 tacctctaac tggtattcca ctcccctata ttagtcaagg tggttctgcc attcttagta
     9601 attttttggg gttgggattt atgttgtgtt gtagtagata tactaacgaa aaaaaaggaa
     9661 cacaaaaatg aattcaaaat aacactttat gaatagaatg tctatttcaa taaaaattat
     9721 gataccttaa agttaatcat taaaaaagga gtgtcataat ttgaaaaaaa caaaattatt
     9781 ggtagctagt ttgtgtttgt ttagcagttt attagctttc accccttctg tttcattttc
     9841 tcaaaatggt ggagtggttg aagctgctgc tcaaagagga tatatctaca aaaaatatcc
     9901 aaaaggtgct aaagtgccaa ataa
//
LOCUS       DAZKCN010000093         9840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000093 DAZKCN010000000
VERSION     DAZKCN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.096"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG8K3_002194"
     CDS             complement(78..2198)
                     /locus_tag="ACG8K3_002194"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP9146022.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG8K3_002195"
     CDS             2428..3606
                     /locus_tag="ACG8K3_002195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP9146023.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8K3_002196"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8K3_002196"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9146024.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8K3_002197"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8K3_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP9146025.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG8K3_002198"
     CDS             complement(4959..6212)
                     /locus_tag="ACG8K3_002198"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9146026.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8K3_002199"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8K3_002199"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9146027.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG8K3_002200"
     CDS             7789..9135
                     /locus_tag="ACG8K3_002200"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9146028.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACG8K3_002201"
     CDS             complement(9443..9829)
                     /locus_tag="ACG8K3_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9146029.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3141 a         1792 c         2012 g         2895 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
//
LOCUS       DAZKCN010000094         9651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000094 DAZKCN010000000
VERSION     DAZKCN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.097"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8K3_002202"
     CDS             168..362
                     /locus_tag="ACG8K3_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146030.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8K3_002203"
     CDS             631..930
                     /locus_tag="ACG8K3_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146031.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8K3_002204"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8K3_002204"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146032.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8K3_002205"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8K3_002205"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9146033.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8K3_002206"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8K3_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9146034.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8K3_002207"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8K3_002207"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9146035.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8K3_002208"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8K3_002208"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9146036.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8K3_002209"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8K3_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9146037.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8K3_002210"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8K3_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9146038.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8K3_002211"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8K3_002211"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9146039.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8K3_002212"
     CDS             7521..8291
                     /locus_tag="ACG8K3_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9146040.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8K3_002213"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8K3_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146041.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3016 a         1961 c         1504 g         3170 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg t
//
LOCUS       DAZKCN010000095         9597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000095 DAZKCN010000000
VERSION     DAZKCN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.098"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..1544
                     /locus_tag="ACG8K3_002214"
     CDS             117..1544
                     /locus_tag="ACG8K3_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146042.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1636..2001)
                     /locus_tag="ACG8K3_002215"
     CDS             complement(1636..2001)
                     /locus_tag="ACG8K3_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9146043.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1998..2363)
                     /locus_tag="ACG8K3_002216"
     CDS             complement(1998..2363)
                     /locus_tag="ACG8K3_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP9146044.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2555..2803)
                     /locus_tag="ACG8K3_002217"
     CDS             complement(2555..2803)
                     /locus_tag="ACG8K3_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146045.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2802..2930
                     /locus_tag="ACG8K3_002218"
     CDS             2802..2930
                     /locus_tag="ACG8K3_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP9146046.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2987..4681
                     /locus_tag="ACG8K3_002219"
     CDS             2987..4681
                     /locus_tag="ACG8K3_002219"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP9146047.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4781..4954
                     /locus_tag="ACG8K3_002220"
     CDS             4781..4954
                     /locus_tag="ACG8K3_002220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146048.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5332..6465
                     /locus_tag="ACG8K3_002221"
     CDS             5332..6465
                     /locus_tag="ACG8K3_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9146049.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6496..6816
                     /locus_tag="ACG8K3_002222"
     CDS             6496..6816
                     /locus_tag="ACG8K3_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP9146050.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6845..7309)
                     /locus_tag="ACG8K3_002223"
     CDS             complement(6845..7309)
                     /locus_tag="ACG8K3_002223"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146051.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7438..8687)
                     /locus_tag="ACG8K3_002224"
                     /pseudo
     CDS             complement(7438..8687)
                     /locus_tag="ACG8K3_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8716..9033)
                     /locus_tag="ACG8K3_002225"
     CDS             complement(8716..9033)
                     /locus_tag="ACG8K3_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9146052.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9059..9379)
                     /locus_tag="ACG8K3_002226"
     CDS             complement(9059..9379)
                     /locus_tag="ACG8K3_002226"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9146053.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3106 a         1881 c         1709 g         2901 t
ORIGIN      
        1 aacctttttt aacggcattt atatgctttt tcagaaatta actgatgatt tcaaaaagaa
       61 aacatgtcat aatatttccc atattgtgca aaaaaataca aggcggaggc tctactatga
      121 ttgaagaata tattgagaaa aatattttaa gacagctgtt tttatgtggg caattctatg
      181 taaataaaga agtcaatcta gaaaagctct ccaatctcct gcatgtttgc aaaaccactt
      241 tactcaatga tatcaataac ataaaaaaag aatttgaaga acagatcgct tatacacatc
      301 gggaaaagga ccgttacact ttatattttt cagaacatat acctcgatgc aagatcatgc
      361 agcaattgtc tcaaaattcg ttgtttttaa aaacttgtct gctttatttg gaagaagatg
      421 aacctgatta tcttcaatta actgaatgtg aattcatctc tgtcagcaaa gcttattcat
      481 taaaaaaaca agtattagct tattttaatg actgcgggat cgaaatcgac cgctactccc
      541 ctcgattcac tgaaatggaa agacgtctgc tgcttcttaa tgtgtcgtat cgtttaggcg
      601 gttttaattc ttgggagcta cctgaatcgt tttttgaacg tgccgatcgt ttcattgaat
      661 ccgtaacgga aaatagtggc agattttatg ataaagaaaa taaagaaatc ttatcgattg
      721 gttttgctat cagtttccta cgccagcaag tgtgtgctgt gacaatagat tcaaaattta
      781 tcgaagaaat caaaaaacgt cctatttaca attatgtcga atcagcatgg gaaaacactg
      841 atttccaaac ctattataaa aaagaagaat tcgcctttat tttgacacta ttcaatcttt
      901 gtaattatgg tttccattct tatcagttga ttgcggaaga ctttcaacag ctacaccaag
      961 tgtttattga caatactccc gaaataaaag aactagttgc tacctttgaa tctcacttca
     1021 atcaagaatt attcggaaac caaccgttcg aacgtgcgct gattcatctg atgcgttcgg
     1081 cttgggataa ctatcaatta ttcatgccag aaaaatttta tcttttaaat gaggaacaga
     1141 caaatttata taaagaagtc cagacaattt tttccagctg gtccagccag ttaccttatg
     1201 atctacggtt gaatcctaat tgcatgcggg cttttgtgat cgaattgagc ggaattctcc
     1261 gtctgacaaa agaacatctg actatctata tcgtcacaaa ttcggatgtt cattatctga
     1321 tttatcggga agccttggaa gctgtaacta ctttcgattt tcaagttgca ccaacaattt
     1381 attcaagtat atctgatata aagaagtatg cgcagcagtc ctcaaaccgg gtattatgtg
     1441 aacgtacatt atatacacca gatgcggtcc aatatgaaaa tattattcct atttcgatca
     1501 atacgatcga tcgagctatc atttcagctg tgcaaaacaa ataaggggct gtgacaaaaa
     1561 tcatgtcaca gcctcttatt tgttgaagat gcccatttct gtaacaatct cttgatacac
     1621 atcaggctca caacctcact tattttggac atctacgggt ccgatctctt tatttaaaaa
     1681 ataagaaatg atggttcgga taactaccaa cgccgctagt tggaaaatat cctgcaaggt
     1741 aggtttgata atcgattcga tgatcccagc tgaaacaaga atttccaaac ttagaagtat
     1801 atagcctccc aacatctttt taatagcatt gttctcgctg actcggtcgc tttgcacttt
     1861 tgtatggaca aataaatatt tcaaaaaatc cttgattgat agacaaacac cccaaaccaa
     1921 taaacagata gcaaagatat caagtcccag tacaagtttt tcaaaatatg gaagaagaaa
     1981 ctgcatcaac tctgtcgtca tcctacctct cctcttcttt gtttttcttt gtcttctatt
     2041 tcctgattca agaaataaga aatgactgtt cgaatgataa taataagtgc aagcttcaaa
     2101 atatctgtcc aagtcggctt gataactgac tcgatgatat cagcaacgac tagtacttcc
     2161 aagcttaaca aaatataact tgctagcagt tttcttgtat ctgcattacg agtattacgt
     2221 tctgcatagt ttttcttgac atgagaccaa tcaaaaagtt tgaatagaac aagaacgatc
     2281 ccgacaatta agataccgat agaacaaata tttaaaatag caatgatcca ttctagaaat
     2341 tcaattaaat ttgagcgaaa catatacgct acctactttc ttttaatttg ttcctattct
     2401 tttattgtaa aagaaagtta ccttgttcgt ctatttaaaa gttgttctaa tgataacggt
     2461 tcgcttaaat ttttcttttt cactctcccc tttttctccc tccatccata aatcgtggaa
     2521 gtatccaaaa caacgtagca agttaaaata aggcttagtc cgtcatcagc ttttcaaaga
     2581 agcactgtct cggtgctttt tttgtaccca aatttctaaa aatagaacca tacacctcct
     2641 caaatctttt cttcttacct accctatttt tatttacagg aaaatcagac acttcttctt
     2701 ttttgtcaaa atttcctttt tcttctcgtc ctatcatttg gcattctaat aaaaacgaac
     2761 tatccttgga tatagaaaga gaaaacaagg aggcgttttt catgagtaaa aaattaatca
     2821 aagtcggcat cggtttaggt ttgcttgccc ttggcgctgc atatctagga aaaaagacag
     2881 gactattcga agatgacagt catttatatg atgaattcga atcaatctag catcctattt
     2941 agttgaatca ctacggacat acttggaaga cattggagga atcacaatga gaaaaagtaa
     3001 agcaataatg attggtgccg gtattgccaa tatggcagcg gctgtttatt tgattcaaga
     3061 aggcaaatgg aaaggggatc agatcacctt ttattcattg gatgatcacg gttcaaatga
     3121 tggtgcaccg accgacactg tagtggatga atattggaat aaaaaccatc cattagaaaa
     3181 ccaaaaaggc tatattgctc gaggcggtcg catgcttaat taccgtacct atgtagattt
     3241 gatggatctg ttaagtcgaa taccttctgt tactgagcca gggatgactg ctgaagaaga
     3301 tacgagagat tttgatgcaa aacaccgaac ctttgacaaa gcacgtttga tggaaggtgg
     3361 caaaggaatc attgatggcg gaaaactggg attcaataat aaagaccgta ttcttctgac
     3421 taaactgatt gctatgcctg attcagaaga agaaaaacta gacaatatca ctatcgaaga
     3481 atatttcaaa gatgatccac acttttttga aacaaacttc tggtttatgt gggaaacaac
     3541 atttgctttc agaacacgta gttctgctca agaattgcgt cgctatatgc atcaaatgat
     3601 ttatgaattc actcaaatcg aacatctagt gggcgtcaat cgaacacgtt acaatcaata
     3661 cgaaagtatt atgcttcctt tgatcgagta cttgaaagaa caaggatgca aaatcatttt
     3721 gaaccgccgt gtcactgact gggaattcaa agaaacaccg atgcaagatg aaatcactgt
     3781 gactggtttg aaaatgacca atacactgac tcaagaagaa gaacacgttg tggtcgatga
     3841 agatacagca gttattttca ctaatgggtc cattacagat tcagctactt taggagatta
     3901 cgaaacacca gctgtagaaa atatggatta tggtgcagct tctagtctct ggaaaaaagc
     3961 cagtgaacgt ttctacaact tgggaaatcc tgacaaattc tttgctgatc gagatgcaag
     4021 cgaatgggtc agctttaccc tgacgacgaa aaaccatttg ttgttgaatg aaatcacccg
     4081 tatcacgact caagaaccag gaaatgcctt gaattcattt atttcaacca caccgatcac
     4141 gccgttaggc caaaaagatg tcaatatgtc tatcgtcgtg catcatcaac cgcattttac
     4201 gtctcagaaa ccaaatgaaa cagttatttg gggttatttc ctttatccaa gacgacgtgg
     4261 cgaatttgtg gataaacagt atattaaaat gaatggaaaa gaaatgctag aagaactgat
     4321 tggacaactt tcaaaagtag atcctggacc agtgaatatt cgtgaaaaag aaacagaaat
     4381 tttcgacagc gtaataaata atatccctgt ctatatgcct tatgcttccg cgttgttcaa
     4441 taatcgtgcc aaaagcgatc gaccaaaagt cattcctaaa cattcgacca atctggcctt
     4501 tacaggagaa tttgtcgagc agccttatca gatgatcttt actgaacaaa gtgctgtacg
     4561 ttctggagaa atcgctgctt tccattttgc gggtatccct atgtcacgtc ttgtcaaaac
     4621 accgcgttac gacaaagata ttcctactct agctcgtgcc gcaaaaaaaa tgtttgaata
     4681 atcaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca ggctaaaaca
     4741 gcttcttatg tttttgattt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     4801 tttaccaaga ggttgtgaaa aagctgccct gcatcaagta ataagagcag ccaagctaaa
     4861 acagcttctt atgtttttga cttggttgga cttattaccg cagatgcaag cttttgaaca
     4921 ccgtttacca agaggttatg aaaaagtcgt ttagtttcga gtatgaagaa aaattttgtg
     4981 aaaatggttc ctcacgtttt tgcataaatt tgacttaata ccgagaaact gatcccttct
     5041 gtcataacct ctttttattt atgtctgttt ctttttgaaa acaaccattt tttcaacgtt
     5101 tactctcttg ccaatccaac atcatctaac agatacaact atttcaatga ttaccattac
     5161 actaaaaaat cggaaactca cctctccgaa cacaaataca taagactata gaatgtttca
     5221 tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc tcctttaaaa tgaaattatc
     5281 tgtagtacta ataatgcaga caattttatg aatctgaaag gagcatctcc aatgaaagca
     5341 gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg tagatgatcc aaaaatatta
     5401 gaaccaacag atgcaatcat ccgtatcaca gcaacagcca tttgcggctc cgatctgcat
     5461 ctttaccatc atggtgaagc agttttggaa aaagggtatg ttgtaggaca cgaaccaatg
     5521 ggaatcgttg aagaagtggg atctcaagtt caaacattga aaaaagggca acgtgtggtt
     5581 atccctttta atattagttg tggtcattgt catttctgcc tgaatcacat ggaaagccaa
     5641 tgtgatgaat caaatccaga tcagttatat ggcgggcttt tcggatttgg caagttgaac
     5701 ggtaatcatt ggggtggtca agcggaatat ctaagagttc cttatgcaga ctctacttca
     5761 tttgttgtac cagataatga tcttcctgat gaaaaagttt tatttctatc agatattttg
     5821 cccacagctt attggagcgt tgaaaacgca ggagtaaaaa aaggcgatac agttgtgatt
     5881 ctaggttctg gtccagtcgg tctattcgct caaaaattcg ctgttatggc tggagcagag
     5941 cgcgtaatcg cagtagatcc agtacagcac cgtctagata aagcggctaa ttacaatggg
     6001 gttgaaactt atttcttaga agacacggca caagctggaa cagaattgta tgaactggct
     6061 aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg acggattaga gcctgtcaaa
     6121 gaaaaagcga aaaatttagt aagcttacaa aacggcacaa tctctccatt acagatggca
     6181 tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg gtgtttatat gactccagct
     6241 tcttcttacc cgctgcaaga attttttatg cgggatatcg ctgtcaagca tggacaggca
     6301 ccaattatcc atctaatgcc aaaaatttac gaaatgattg cagaaggact attcgatcct
     6361 acacaaatca ttactcattc gctgccacta tcagaagcag cacaagcata tgagatcttt
     6421 gacaaaaaag aagataaaaa tataaaagtt gttttgaaac cttgaattca gtaaaaaata
     6481 caggtgattt tctttatgcc caaaatcaaa cgaaataccc atgagatcga aataggaaaa
     6541 gaagaagata tcgaaatacg aggacgtcgc ttccgcttct atttccaaaa tcgttacaca
     6601 ttgatctcac tggctgtcga tttattaacc gggattttct atatttttgg aagccttgct
     6661 tccttaacta aaatccccga tcgatatggc acctattttt atttggctgg tgctattttc
     6721 ttgacgattc gtcctattct cagaatcgta aggaacattt ttatttacga cgaaaaaaaa
     6781 gcaatgaaga gaacaagcaa aaaaaaagag gcatgagatt tgaaaaaatc tcatgcctct
     6841 tttttcaccc tatcgtatca ctttgataca gacgagcggt cgtcccactt tcttccttga
     6901 ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt ggtatgatct gtaattaagt
     6961 aaagtttttc gtacccaagt tgatgcattt cttttctgat ttccgctaat atccgcttgg
     7021 ctatcccctg cttacgcttt tccggttcaa caaacagggc acaaagattt ggtgtcaaat
     7081 cagggcggtt atggaagtcg ttttcaataa cgcccgctcc tccgacgatg gtttcttttt
     7141 catctaagac cacataccat tgaggaatac tttccttatt agcgattcca gtacgaatac
     7201 tttcctcata cacaacagca ggaactcccc attttgatga aaaccacttc gcactttgtt
     7261 ttactaattc tgggtgttct cttaatttga tccagttata tgtaaccaat gtttaaacct
     7321 ccgactaaaa taaaacgttt acaaaaatta tacaagtctt tatgaaaatt agcaatgaca
     7381 acaatagtga tgtaaaaaaa acggaaaaca atcacttgtt ctccgttttt tgattaatca
     7441 agcatctaaa gcttcttttc ttctttcttg gatcgtagct accttgacga acggtagata
     7501 gatcaaaata gaaagaacca aattgacagc ggctagtaca ccacctgcaa tactttttgt
     7561 tgccaataca ccgccaataa ttggtggagt tacccatgga ggcataacgg tagctgctgg
     7621 cactaaacct gtagaagtcg caaagtaagc aacactaact aagaccattg gtgtcaaaat
     7681 aaatggaatg aacatgatcg ggttcaacac gataggtaac ccaaacatcg ttggttcatt
     7741 aatattgaag acacctggcg caatcgacaa gtttgttaca accatatatg gtttgttttt
     7801 acgtcctact aaataaatag ctaacagtaa acctaaagtt gcacctgttc ctcctaagtt
     7861 gacaaagctg tcaaagaaag gtttgttcac gataaacggt gcgctatgtc cagctgcgat
     7921 tgcattaacg tttgcttcaa tggctggcgc attgattgtt tgcatcaaag gatcgaccat
     7981 attcgctccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcagtaaggc
     8041 agatggataa gagtttgcaa gtcccataaa tggttcttgg accactgtat agaaagaacc
     8101 tacgatatca tcaacaccga atgcagcaaa aattgcagca actaaagcaa acgcagagat
     8161 agtgatcatt gctggtagta atgaagcaaa tgcttttgca actgccggtg gcacaccatc
     8221 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
     8281 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
     8341 agcgaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
     8401 agctaaatga taagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
     8461 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
     8521 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
     8581 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
     8641 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
     8701 tttctttact gtagattacg cgttcaatcg acgatacaga tcaatgatct cttttgctaa
     8761 atctgtaaaa gctatggatg tcatcagatg atcttgactg tggactgtca aaagcgtgac
     8821 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
     8881 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
     8941 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
     9001 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
     9061 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
     9121 cagccatatt gattatatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
     9181 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
     9241 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
     9301 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
     9361 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
     9421 aagtagcgtg ccaactttga aagcgcttta aaaagttgat atcatcacat ttaaggtcta
     9481 taccaagttg catacacact aaaaaagaaa gtgcacacta aacacacaaa ttaattttta
     9541 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaac
//
LOCUS       DAZKCN010000096         9507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000096 DAZKCN010000000
VERSION     DAZKCN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.099"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..974
                     /locus_tag="ACG8K3_002227"
     CDS             168..974
                     /locus_tag="ACG8K3_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9146054.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            986..2206
                     /locus_tag="ACG8K3_002228"
     CDS             986..2206
                     /locus_tag="ACG8K3_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146055.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2196..3782
                     /locus_tag="ACG8K3_002229"
     CDS             2196..3782
                     /locus_tag="ACG8K3_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9146056.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3821..5959
                     /locus_tag="ACG8K3_002230"
     CDS             3821..5959
                     /locus_tag="ACG8K3_002230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9146057.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSVIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6247..7638
                     /locus_tag="ACG8K3_002231"
     CDS             6247..7638
                     /locus_tag="ACG8K3_002231"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP9146058.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7697..8506
                     /locus_tag="ACG8K3_002232"
     CDS             7697..8506
                     /locus_tag="ACG8K3_002232"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9146059.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8478..9467
                     /locus_tag="ACG8K3_002233"
     CDS             8478..9467
                     /locus_tag="ACG8K3_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9146060.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
BASE COUNT         3285 a         1442 c         1902 g         2878 t
ORIGIN      
        1 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
       61 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
      121 aaatggcact tgactgtaat tcactgaaat cgcttggagg aacacaaatg tttaaagatt
      181 tgtatttatt tatgaaagac atttatcaga atagaaaatt actgctacag ttttcgatca
      241 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
      301 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
      361 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
      421 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
      481 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
      541 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
      601 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
      661 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
      721 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
      781 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
      841 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
      901 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
      961 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
     1021 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
     1081 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
     1141 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
     1201 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
     1261 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
     1321 aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
     1381 tgaatttgct gatattggcg accatattta tcaacctgta aaaacctatt caagtgggat
     1441 gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat cctgatattt tgattgtgga
     1501 tgaagcattg gcagtaggag atcttgaatt ccaactgaaa tgtatggaaa aatttacaga
     1561 aattaagaac tcgggaaaaa cgattttatt cgtttctcat gatattagtt cgattcgccg
     1621 tttttgtgat cgttcctatt ggttgaaaaa tggagaagtt gtcgaatatg gtgatacgat
     1681 ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa tcagttaaaa cagtagatcg
     1741 aaataaaaac caagtagaac gcttcgcagc tccagatatc gtagacgtga cgaaagccga
     1801 attgttggat aaaaataaaa atccagtgga tatgctggaa caaggagaaa aactatacgt
     1861 taaagtaacc tatgaagtaa aagacgatac aatcaaaaat ccagttttag gaattgctct
     1921 gcgtacagtc gacaattttt atgtgtgcgg actcaatact ctactagatg gtatcaagat
     1981 cccttggaaa aaaggtgaga atatctttta tctggagtat gaaaagttag gtttgttagc
     2041 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
     2101 caagacaaaa tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
     2161 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
     2221 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
     2281 tagagtttgg tcccggaaac ggtcgattaa cgaaacattt gatcggagca aaacaatgtg
     2341 aagtgagcat tgttgagcta gataaggaat tattcgattt tgtcagcgaa tttgcccaag
     2401 atggatttta tggagatatc gaaagttttg aatgggcaaa ctattatgct ggacagacat
     2461 ttgattacgt cttgtttgcg gatgttttag aacatttagt agatcctgga aaaacattga
     2521 agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc cctaacttag
     2581 cacataattc tgtcatgatc gatttgttca ataatcaatt gccttgggct tcttatggtc
     2641 tcctagatga aacccataat tctttttata ctcatgatgg tttccaaaaa gtttttgaaa
     2701 aagccggatt attcattaat attgaagatt acttgtactt ggctgtagga gatacagaat
     2761 taaaatcaac ttatgaagag ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat
     2821 ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagta caaacttcca
     2881 ttgcacagcc acaaaactct aattatgtaa aagtactaga agtaacccag caaacgaaac
     2941 aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa
     3001 gtttccccgt gtcagaagca acagaaagaa tggtgtttcg cttcgcacag caacctagct
     3061 ttatcgaatt cagtgcagaa gctgctggag aaaaactcat atttatcgat tcaaatgctg
     3121 tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaaatacca gagttcgctc
     3181 taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat
     3241 tgacaccaac aatgaaagaa ttgctggaaa caattcgtcc tatggctgaa gtcacaaagc
     3301 agctttctga gaaaaatgat gaattagaaa gagaaaatca gcatcttctt gaggaaaata
     3361 caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg
     3421 atgaatggac gatcaaacat agactaggac gacgtaagaa aaaaacatcg aaaaaaatac
     3481 aagaaaaaga gctttctata tgtattgatg agaaaatatg ggatgcagaa acaaaaatat
     3541 taaaaatcat cggatgggga atcgctaatt cggatcgcca gccattgtcc tataagttat
     3601 cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagatgaa gtcaatcaag
     3661 cagaacagct cgcaaaagga acagaagcgg gatttgaatt acgaatcctt tgcgagcaag
     3721 aacgttcatt cttagtggaa gcagtagctc aaaatggtca atcatggatc attgaaatgt
     3781 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
     3841 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
     3901 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
     3961 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
     4021 gacccaaagc ggttttaaga tacgtttatc cgggaaaatg agaggaaaag cgatcttaga
     4081 ttttcaaacg gcaaaacatc aaaatggcat agctgtgaaa ctaaatggta aataccctta
     4141 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
     4201 ttatgcaagg actcatggcg taaaaaaaac aatccgccgg gtcaaactgg aattgaaacc
     4261 aggatcgatc gactatgcag agtggatcag tcgtcacgag aaacctgatc tgaaagagca
     4321 aaggaaagaa gcacaagggt ttgcataccg tccattgatt tccatactta tgcctgtcta
     4381 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtattggatc aaacgtatga
     4441 tcactgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
     4501 ggaatcctat caagcaaagg atgatagaat caaagttgtt ttccgacaag aaaatggaca
     4561 tatcagtctg gcaactaatt ctgcgttgga aatggcagaa ggagaattta tagcgctctt
     4621 agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacat
     4681 acatccagaa ctggatttga tctacagtga cgaagataaa atcgatgcag atggcaatcg
     4741 ctttgatccg cacttcaaag cagactggtc tcccgataca ttgatgggaa ataattatat
     4801 ctctcactta ggcgtatatc gaacaagtat tgtgaaaaag cttggcggtt tccgtaaagg
     4861 ctacgaaggc tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga
     4921 tcatatttac catattgacc gagtcttgta tcattggcgg acgatcccag gatcaaccgc
     4981 aagtaatggt gaagccaaaa gttatatcta tgattctgga gtgaaagcat tgacagatgc
     5041 cctgtctaga aggaatatca aaggaagcgt acgtcctggc agaatttctg gattttatga
     5101 gattgcttat gacgtcttgc aagaggattt ggtcagcgtg atcattccaa cgaagaatgg
     5161 ctatgaagat ctaaaaactt gtgtggattc ggttatcgaa aaaacgtctt atccaaatta
     5221 tgaaatcatt attgcagata atggaagtac agatcctaaa atgcaggaat tatttgcaga
     5281 atacaagcat cagctgaaag accgatttat cgttgaatta attgacattc cattcaatta
     5341 ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc aaatactttt tattcttgaa
     5401 taacgataca gaagtgattg aacctgactg gatgacagcg atggtttctt atgcacagtt
     5461 tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc
     5521 aggagtatta ttaggaatcg gtggtgttgc aggacatgca ttgaataact atgatcgaac
     5581 acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
     5641 ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat ggatttgatg aaacattaga
     5701 agtagcattc aatgatgttg acttgtgttt gaaagtttat gaactgggtc gttacaatgt
     5761 ctatgcacat caagcagaat tgtatcattt cgaatctaaa tcaagaggtt atgaagatac
     5821 accagaaaaa caaagacgtt ttgccgggga aataaaaaaa atgcaagata agtggccttc
     5881 ttatattgct catgacccat tttataacga taatctaact aaagaaggaa tcggcgattt
     5941 ttcattgaga cctgattgat taaaagaact gtattttcat ttttattgat gaaaatacag
     6001 tttttttagt atatgaaaat tgtaacttgt gaccgaagag atgataggaa gaacacccat
     6061 tcagtaggaa gaatgtgata cgatttttgt ccaactctct gttaggcaga aatatacata
     6121 taaacataca atagtcacct tttctagttt cttttaattt attttacaat ttggttattc
     6181 actaggtttt taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt
     6241 gtatatatga ataaaaatgg ggagtggaat gcggcacgta ggatattcat tatcatcatg
     6301 gatgtacttg tattgaatgt gtcgatctat cttagctttt tgctgaagtt ccaaggagaa
     6361 gtcccagcaa gaaacttaga gacctttcag cattcagcag tttttatttc cattatcttt
     6421 attgcgttga atattttgtt aggagcttat gtattctata gccgagtgat cagtgatatc
     6481 gtcttcatta ctgttattgg acaggtgctg atagcattgg ggatcatggt agttactttt
     6541 gctggacgtt ggtttgcatt tccgcgtggc gtcatcgtgc tctcgtttat acttggcacg
     6601 atattactta gcgtttatcg gatagttgtt tataaactat acttaagagt cagtcgtgat
     6661 aagaaagtcc ttatcgtagg tttggagaaa gatgtcgcac cagctatcca aaactttcaa
     6721 tctaagaaaa acaacaaaca taaagttgaa gcagtagtga tcgacaagta ttacgaaaac
     6781 acaaaaaaat tgattgatga tatcgatatt gtctacttgg cttctaaaat cccagaagac
     6841 gaaaaaatca atatttatca attagtaact aaaaaagaga agaagctatt tttaaataca
     6901 acttttgaaa atttaacgat gatcaatccg aacatcatga atttcgaaga tgaaagtatc
     6961 attgaagctt ccggtttcaa aattcagccg gaatatgaat tattcaaacg tctttttgat
     7021 atcatcgttt cgcttatttt actgattctt gcttcacctt ttatgctaat cacagcgatt
     7081 ttggtaaaaa caacttcacc tggaccaatt atttataaac aagtacgtat tacgaaaaat
     7141 caaaaggaat tttcaatcta taaatttcgt tccatgagtg taacagctga agcaaaatca
     7201 ggaccggtct tggcaaagag caatgatgca cgtgtgacac cagtaggcaa atttatccgt
     7261 gccgtacgtt ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatc
     7321 gtcggaccac gtccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat
     7381 tattatttaa gacacaatgt acgtgcaggg attaccggat atgcacaagt atacggaaaa
     7441 tatgtatcag attataatag taaactacgt tttgatttgt tgtatatcaa aaagtattcc
     7501 ttactattag atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtcc
     7561 tcacaaggac tagatgaaga tgaaaatatc cgttcgttag cagaaatcga cttttcacaa
     7621 gagaaaattt atcgctagat gggaaaaatt ttgagcaaag aagtaggaga gaacattgga
     7681 aaataaaaat ataagtatgc tgctttcttt atatgataaa gaaaaaccag agtatttaaa
     7741 ggaagcatta actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta
     7801 tgacggccct ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc
     7861 tcacttgttt acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat
     7921 tggattagaa catacgaaaa atcgcttagt agccagaatg gatacagatg atatcatgga
     7981 accccaacga ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt
     8041 tgggagtaat attgaggaat ttactggcac gcttagcaat gttatcggaa gaaggattgt
     8101 acctgaatat aatgatgaaa tccgtgtgtt ttcgaaaaga agaaatccct ttaatcatat
     8161 gacagtcatg tttgataaaa atgctatttt agaagtggga aactatcgcc cattaattgg
     8221 atttgaagat tattatttgt gggtaagact gttgaaggcg ggatataaag ggtataatat
     8281 ccaagagatt ctagtccatg ctagagcagg tgctgacatg tatgctagac gtggaggaag
     8341 taaatatctt ttaccaggaa ttaaaggccg atatcgcgta tggaaagatg gtctaggatc
     8401 actaaaagat tttctgatag ttatttgcgg acatcttgta attagtttat taccgaataa
     8461 aattagggga aaagtatatg aatcaaaact tagaaaaaca aactaaactt tctataatca
     8521 taccttttta caatgcaaaa aatactttaa aaagagctgt agacagcgtc ttaacgcaag
     8581 gttatcccaa tattgaaatc ttgttaattg atgatggatc tacagatggt agtagtgaca
     8641 ttggtgacga aattgctaat ctagagagta aagtagcatg ttatcacttg gaaaacgctg
     8701 gtccatcagt tgctaggaat tgtgggataa aagaagcaac tggaaatttg gtaggatttt
     8761 tagatagtga cgattatttt ttacctgatt gttttaataa tgttttgaaa gactaccata
     8821 ggaattaccc cgatatcatt gcttttggat taagtaaagg aaaaaatata gaaaatgcaa
     8881 atttatttgt tcccgaaaag aagattggtt gttcaagtga agaagcaatt gagcaaatgt
     8941 ttagaagtaa ggcagtggat ttttacgtat ggaataaatt ttttaaaagg gaattattca
     9001 atcatatagt ttttccagaa aataaattat atgaggatat ggttcctatg tatgaagctt
     9061 ttagagcggc tagtaaaatt gatattttgc cattttcagg tattttttat tatcaaaata
     9121 atgatagcat cgtttatcag tcttttaatt caaaacagta tgataatatt ttgcaaagaa
     9181 aaatattagt tgatgaagta aagaaagatt atcctcgact tattaatttg gccaatgctc
     9241 gattagtaga cggatacctt tcaacagggt ttaaaattac tggcaagaat caaaatcaga
     9301 aagaaaagca aaaatattat aatcagtcta aaagagaaat aaaaagtata tattcaaaaa
     9361 taagaagtaa caatgagata tcaattgcta aaaaattagc tttattttta tacttaatta
     9421 atcctaaaac ttattttatt ttatataaaa aagttctaaa aaaataaagt ttttttcgat
     9481 atgttatttt ttatgtttat taacttc
//
LOCUS       DAZKCN010000097         9497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000097 DAZKCN010000000
VERSION     DAZKCN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.100"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..2069
                     /gene="tdc"
                     /locus_tag="ACG8K3_002234"
     CDS             234..2069
                     /gene="tdc"
                     /locus_tag="ACG8K3_002234"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP9146061.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2374..3852
                     /locus_tag="ACG8K3_002235"
     CDS             2374..3852
                     /locus_tag="ACG8K3_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9146062.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3818..5149
                     /locus_tag="ACG8K3_002236"
     CDS             3818..5149
                     /locus_tag="ACG8K3_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9146063.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5263..7815
                     /locus_tag="ACG8K3_002237"
     CDS             5263..7815
                     /locus_tag="ACG8K3_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP9146064.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7822..9261)
                     /locus_tag="ACG8K3_002238"
     CDS             complement(7822..9261)
                     /locus_tag="ACG8K3_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146065.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9236..9418
                     /locus_tag="ACG8K3_002239"
     CDS             9236..9418
                     /locus_tag="ACG8K3_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146066.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
BASE COUNT         2986 a         1579 c         2026 g         2906 t
ORIGIN      
        1 ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt gttcgctttt
       61 ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa aatctacgtg
      121 gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga ggtgttacag
      181 ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag
      241 atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa
      301 tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat attccttcag
      361 atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc
      421 atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc
      481 attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag
      541 cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa
      601 cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg
      661 gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc
      721 gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa
      781 tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct
      841 ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc
      901 aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag
      961 atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa
     1021 tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc
     1081 taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa
     1141 tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg
     1201 ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt
     1261 atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg
     1321 acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc
     1381 ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata
     1441 tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag
     1501 atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct
     1561 ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt
     1621 caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag
     1681 gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat
     1741 tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg
     1801 atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta
     1861 catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa
     1921 cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc
     1981 cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg
     2041 aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag
     2101 acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt
     2161 catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt
     2221 ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt
     2281 ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa
     2341 aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa
     2401 atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt
     2461 ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg
     2521 tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt
     2581 ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg
     2641 ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg
     2701 tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc
     2761 attttagtcg tttattggat catcactttt ttgaatttga aatttgatat ggcaaaaatc
     2821 ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta
     2881 gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca
     2941 tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc
     3001 atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca
     3061 gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt
     3121 ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc
     3181 gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg
     3241 tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca
     3301 gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg
     3361 aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca
     3421 atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta
     3481 aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga
     3541 ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag
     3601 agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc
     3661 ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc
     3721 attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa
     3781 gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg
     3841 gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt
     3901 atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt
     3961 gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa
     4021 tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt
     4081 ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc
     4141 aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag
     4201 cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga
     4261 atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg
     4321 attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat
     4381 cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt
     4441 cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa
     4501 gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac
     4561 gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt
     4621 agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat
     4681 tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata
     4741 tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt
     4801 acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt gtctacattt
     4861 ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa cgagttgttt
     4921 tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg
     4981 gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt caattgcttt
     5041 cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta tctggtttgt
     5101 attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg cccagataca
     5161 ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg aaaaaacttc
     5221 cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg gtacagattg
     5281 acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc gcaagaagaa
     5341 cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca gcagacaaaa
     5401 cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt gatcattgcc
     5461 tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt attcgtagtg
     5521 atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc attgaatggt
     5581 ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca agaaaaaatc
     5641 cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga tgtcgttcca
     5701 gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat tttgacggga
     5761 gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc gatcggcgac
     5821 cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt
     5881 gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca atcggtcgaa
     5941 caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc
     6001 attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg tatggattgg
     6061 catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga aggattgcca
     6121 gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc
     6181 atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg ttcagataag
     6241 acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc
     6301 tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga acagaaaaca
     6361 gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga aacagccaat
     6421 ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa atatatggca
     6481 acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc agaagtatta
     6541 tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac agctgcagaa
     6601 tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga ttcgaaacag
     6661 gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg attgatcgat
     6721 ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg gatctcagtg
     6781 aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca aattggctta
     6841 aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga tgaagagttg
     6901 attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca taaactgcga
     6961 attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat
     7021 gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa aggtagtgaa
     7081 gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac gattgcaaaa
     7141 gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa tttcttcttg
     7201 ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa tcgcccgcta
     7261 cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac tttatcttat
     7321 gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag agatccaaag
     7381 caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct actgatcatg
     7441 gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca aaccattctt
     7501 ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga gttgtccaag
     7561 ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt
     7621 gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat cggtacagta
     7681 agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct attcttagtt
     7741 gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga
     7801 gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag tcaaacgatt
     7861 gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta gagaatctag
     7921 agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa gatcggttag
     7981 ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct gctgcaagaa
     8041 cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat tcggtttgat
     8101 agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat ttggttcatt
     8161 gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat acaagtgtgc
     8221 atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg cttcaatcaa
     8281 tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca gagatgtttt
     8341 ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt cgaggacctc
     8401 taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt
     8461 ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa gataaatacc
     8521 ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg tgctgttggt
     8581 atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga taaagaacca
     8641 acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga ggtttcttaa
     8701 aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt tgttatgagt
     8761 gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc caagaataca
     8821 agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct gattgatttt
     8881 agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa aatcagggac
     8941 agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg attgtttgaa
     9001 aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac tgattcctgc
     9061 ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt gaaggtcaga
     9121 aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg ttatctttgt
     9181 atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt cggtaatgat
     9241 ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata cggagaaaat
     9301 aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg gagcatttac
     9361 tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca aaaaataggt
     9421 gggcaaacaa taataaacat tttaatagta gatggtagca agaccaacac aggcggattg
     9481 aacatttaag agttgat
//
LOCUS       DAZKCN010000098         9431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000098 DAZKCN010000000
VERSION     DAZKCN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.101"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..2066
                     /locus_tag="ACG8K3_002240"
     CDS             138..2066
                     /locus_tag="ACG8K3_002240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP9146067.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2204..2659
                     /locus_tag="ACG8K3_002241"
     CDS             2204..2659
                     /locus_tag="ACG8K3_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9146068.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2739..2867
                     /locus_tag="ACG8K3_002242"
     CDS             2739..2867
                     /locus_tag="ACG8K3_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146069.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3129..3398
                     /locus_tag="ACG8K3_002243"
     CDS             3129..3398
                     /locus_tag="ACG8K3_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146070.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3798..4052
                     /gene="rpsT"
                     /locus_tag="ACG8K3_002244"
     CDS             3798..4052
                     /gene="rpsT"
                     /locus_tag="ACG8K3_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9146071.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4141..5187)
                     /gene="holA"
                     /locus_tag="ACG8K3_002245"
     CDS             complement(4141..5187)
                     /gene="holA"
                     /locus_tag="ACG8K3_002245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9146072.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5225..7498)
                     /locus_tag="ACG8K3_002246"
     CDS             complement(5225..7498)
                     /locus_tag="ACG8K3_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP9146073.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7491..8030)
                     /locus_tag="ACG8K3_002247"
     CDS             complement(7491..8030)
                     /locus_tag="ACG8K3_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP9146074.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8166..8384)
                     /locus_tag="ACG8K3_002248"
     CDS             complement(8166..8384)
                     /locus_tag="ACG8K3_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9146075.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8381..8626)
                     /locus_tag="ACG8K3_002249"
     CDS             complement(8381..8626)
                     /locus_tag="ACG8K3_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP9146076.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8742..>9431)
                     /locus_tag="ACG8K3_002250"
     CDS             complement(8742..>9431)
                     /locus_tag="ACG8K3_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9146077.1"
                     /translation="EKRTMIEKISKKILASPKLIIWMSMIVLIFCIASGFLLSNLLFY
                     RETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGSVKEPGIYSFSSEE
                     RVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEHLDQLASSEGKQPT
                     ADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDLQEISGIGEKT
                     VEKLKNFVTIK"
BASE COUNT         2918 a         1838 c         1645 g         3030 t
ORIGIN      
        1 ataatcatta taaataataa aaacaacatc aagcttaatc accaaatgat aaccattatc
       61 gttctcattt agctgttatt attatcaatt acatttttat tatgctatcc ttgttttgac
      121 aaggaggtct aataacgatg actaatcaga aaaaagcttt tgcagcaaca atcggctgcc
      181 ttttatttat gatcttcggt tttatcttcg accgattctc tcttaccatc accccacttt
      241 tttatatcag tgccattatc atcggtggat ataaacaaac atcggaaggc ttgaaagaat
      301 tgtggaatga tcggacactg aatgtggatt tattgatggc tttagctgct attggtgcct
      361 gtttgatcgg taattacttt gaaggagcaa tgttgacatt catcttttgt ttaagcggtg
      421 cattagagga atacacaact aataaaagtc aaaaagaaat tactgctttg atgaatcttc
      481 aaccacaaaa agcccaatta ttaaaagaaa atggacaaat gcaagaagta gatgtttctc
      541 aactaaaggt ggataacttg gtatttgtcg ctaaaggagc agctgttccg atagatggct
      601 ttttagaaag cacacaggca acgatcgatg agtctgcatt aagcggtgaa tctgtgcctg
      661 tagaaaaata tttaggcgat gagatgtatg caggaacgct gaatcaagga aatcccatca
      721 cgattcgagt aacaaaaacg agttccgaaa ctgtctttgc taagattatc caattagtag
      781 aagaagcgca gaatacgcca actcaaacag cttcatttat cgaacgaatc gaaaataatt
      841 atgtaaaatt gattttgctt gctgttcctc taatgatcct tctcccccac tttttcctag
      901 gttggagttg ggatgaaagc ttttatcgag gaatggtatt attagttgtg gcttctcctt
      961 gtgcgcttgt cgcatctgct acaccagctt ctctagctgc tctttcaaat tctgcaaaaa
     1021 atgggatttt aatcaaaggc ggtatccatt tagaaaagct ttctgaatta aaagcgattg
     1081 cctttgataa aacaggaacg ctcactaaag gaaaaccagt tgtaactgac agctttttct
     1141 ttgatgaaaa ggatttggca caacaacttc ttgttgctat ggaacaaaaa acgacgcatc
     1201 cacttgctca tgcgattttg caatatttcg attgcacaat acctgaagaa atccaatact
     1261 taccaataga agaaattact ggttttggat tacaaactac ctatttaggt gcgcaatgga
     1321 aagttggcaa gcacgcatat gatcctgaga cgatgataat cagcaaagag attgcggaaa
     1381 tgatcgaacg tttggaaaat caaggcaaaa ccgtgattta tctttctaaa gatcagcagt
     1441 taattgctgt tttaggtctg ctagatattc caaaagcaaa tacccaacaa gtaatttcct
     1501 actttaaatc ccaaaacatc catacaagta tgataaccgg cgaccattct ggcacagcaa
     1561 aggccattgc tgaacaagta gggattgaac attactatgc aagctgtact ccggaagaaa
     1621 aaacacaact agtcaaaaaa gagaatgaac tttaccaagt aaatgcgatg gttggtgatg
     1681 gagtcaatga tgcgcctgct ttagcagccg cttctttagg tgtcgctatg gggcaaggga
     1741 cagatgccgc tatggatatc gcagacatcg tcttaatgaa aagcgactta aataaatttg
     1801 tctacagtca tcggctatct ttaaaaatgc ggcggatcat caaacaaaat attatttttt
     1861 cgattcttgt tattctcttg ttgattgctt ctaacttcct gcaattttta aatctacctc
     1921 ttggagttgt aggacacgaa ggaagcacaa tattggttat tctgaatggc ttgcgtttac
     1981 taagaccttt acctgatctt tctgattctt caaaaaaatg taagtcttgt ccactataca
     2041 aagcgcggct gtcttctgtc aaataagcaa agtgtttttt tatgattttg tttattttat
     2101 gaaaaaaaca gcttatgaat aggtcttgaa ggatgaattc ctacttttgt aactttctaa
     2161 aaacatgtta tgattgtttc gaagaaagtg aggaatatat ttcatggaaa gttggctttt
     2221 tttaggaatt attttacttg tagcaatttt agctaaaaat caaagtctga tgatagcggc
     2281 agcttttgtt ttgattatga agctcatacc agcctcccgt tcctttttcc cttggcttca
     2341 aggcaaagga atcaatattg gggtgacgat catcacagcg gctattctaa tccctatcgc
     2401 aacaggagaa atcggtctac gtgatctatt agaagccttt aaatccccta ttggctgggt
     2461 agctgttttt tgcggtgggc tagtggctgt cttatcttcc aagggtgtag gattgattgc
     2521 ttctgatcca caaatcactg ttgcacttgt tttcggcacg attttaggtg ttgtcttctt
     2581 aagaggaatc gccgccggac cggtcattgc ttctggtatc acttactgta tcttacaagt
     2641 atttgcttta ggaaaataga acaaattttg tctattttag gagattctta acacccccaa
     2701 tttactgatc cgtgatatac tgacaacgag gtgatacaat gttcgaaaaa ttaaagtatt
     2761 tcataggaac aatcggctgg tttcttctag cggtactttt gattctgggc ttagcttatc
     2821 tattatttcc tgatttgata caaaatgtgt ttgatactgc tttttgattg acctattaaa
     2881 acgatatagg ggatgctcct gcgatgcagg ggcttttttt aatcaatgtt ttgtaataat
     2941 ttaggccgtg ccaatgagac ttcctataga attcgatgct cttttttctt tttggctctt
     3001 ctgctctttt ttgttatact agattacagt ggacaaaaga aagaatgatc gcatccactt
     3061 caacgaatat caaaatggaa gaattagtgc ctgttttcag gttactttga aaagaaagga
     3121 agacgcttgt ggaaactttt gaaggtcttt taattgatac gtacgtttta caagaacagc
     3181 caatgatgct gactgggatc aacttgcagc ttgaggaccg tgtgatcaat ggcctgctta
     3241 aagatcgcta tttggcaaaa tatatcttac gtttgcccct aaataaatat gtgctggaag
     3301 taagtggcaa atggaaccgt cggaaccagt tggtcataaa aaaaatgttg ataaaaaata
     3361 tcgatgaata cattaaaata atcggtacac cagaatagcc tttactgatc aaaggatact
     3421 ggttataaaa taactgcagt ttcttaatag aaaaacaaag aacaacaggg aatcgtatcc
     3481 cttttcaaaa aaaactttgc aaacggttct ctttgttata taataactta gtaccaaaca
     3541 ttcccattca aactttgttt aaatagataa ataaaaatct agtttggttt gacgctattg
     3601 cctcccacaa tagcgtcttt ttgatttttt tcagcttgta ctatccgttt ttactgaaaa
     3661 aaaatcctaa taaaagactg ttaaggaaaa aaacttgtca tgttcatatt gcaaagcacg
     3721 ctgcctttag gtataataat gaatgttgaa ttatgcggta acacgcgtaa gaacttaagt
     3781 tatcggaggt gaaactaatg ccaaacattg aatctgcaat caaacgtgtt cgtacaagcg
     3841 aaaacgctaa tgtgaaaaat tcatctcaaa caagcgctat gcgtactgca atcaaaaaat
     3901 ttgaagatgc tgtagcttca ggtgctgaca acgtagatgc gttatataaa gaagctgtta
     3961 aagctattga tatggctgaa tcaaaaggtc taatccacaa aaacaaagct aaccgcgaca
     4021 aatctcgttt aagcaaaaaa atcgctaaat aaaagaatcg gcttcggccg atttttttat
     4081 ttgtcaaaaa aaggacaaaa acaaacgacc ttcccgcatg ctttgccctc gtctttcttc
     4141 ttatcgattt cttaacaact gccctgtgcg caaaacgaat agttgaaaac ttaattcttt
     4201 ttcgatttgt cccgttttga tttgataatc catctcaact aattgatcaa acaactgtac
     4261 cagcaaatgt tcatcgaatt tttttacttc ctgcatggca agttttacac gatatggatg
     4321 gattttcaat gtttcagcaa tatttgcctg ctgataacca atctttacaa gaaattttgt
     4381 ctgtaaaaat aaacgtaatt gagaaagcaa aatcgcatta attttgattg tttcttcccc
     4441 ttgcataact aaatcttcgt ataaacgcaa agcttgttcc gtcttccctt ttaaaatata
     4501 ttgggtcatg tcaaaaatat tatgttctaa cgttttagga actaattctt ctacttctct
     4561 taccgtgatt tgctgttgat tttgtccata aaggaccaat ttctgtaact ctctcatcat
     4621 tttggataaa tctaagtcgg tcaacctcaa gaataaatca attgcacttc gttccatttt
     4681 gattgaggaa ttctctaagg tattcatcag atattgacgt acttcttttt cattcaactg
     4741 ttggacattg atgataattg ctgttttttt caattgtttc gttacttttt tccgttcatc
     4801 caacttttca tatggcgcaa agaatactaa tacagtcgat tctaatggtt ctttcaaata
     4861 agccgtcaat aaatcagtat tttgttcgat tccattgttt actttttcac ctgttaaaaa
     4921 ataaggattc tctacgaata ctaaccgttg atctccgaaa aacggcaacg tctctgcttc
     4981 agctactacc tcatctaaac ttgcattttc catgtcaaaa cttaaaaaat ttaagtcatc
     5041 ttcgccctct aattggattt ttttgacaat ttctgtccga acttgatctt ggagaaactt
     5101 ttctgttcct aaaatcaagt aacaagatgc aagtttttct ttacgtattt tttgtaactc
     5161 tgcttgtata ctcaaattac tcaccacttt caaaaattat cctaccatga gcagatgaga
     5221 attactagtc tatcattgtt tccactagcc catgaggatg aaaaactgga tgccagcgat
     5281 aggtgatcat tccttgttga tccgttcgcc atgtttttat ctgattttct tctagtgtct
     5341 ccaatacttc gctgtgcggg tgaccataac gattatttac gccgcaggat ataatcccat
     5401 gttttgcttc aatatgggaa agaaaatctg tgttacttga cgtacggcta ccgtgatgac
     5461 ctaatttgac tacatcggct ttcagattag gataaatcaa aagcaattgt ttttccattg
     5521 cttgattcaa atctcctaaa aataaaaagc gcctattgtt atattccatc catagaccaa
     5581 tcgaatcttc atttcttccc tctccctttt caaaaggagt caatacataa agatcataat
     5641 aaccgctgat cctgtcccca tgtttgattt caatgatctc agtaccattt ttttgagctt
     5701 cagataaatg cctttgtaat ggctcatttc gtccagcgcc ccaaccgata taaatttttt
     5761 tgatagtaaa atgctgagat aacgtgggta attctcccac atgatccgta tcgtcgtgtg
     5821 tcaagatgag tttatcgatt acacttatcc cctgaccttt tagatatgga acgatatttc
     5881 tttcagcagg aggacgtatc ataccttgcg cccacttttc tctttcaaag tgtaactgac
     5941 ctccagtatc gatcaaaatc gtttcttttc gaaaaggact ttgcaaaaag atactgtctc
     6001 cttgaccgac atcgataaaa gcgatcgttg aagaaaagcg taaaaatggc aaagtcaaaa
     6061 aaataaaaga aaaaagacca acataaaaca agtgtttcac aggttgttct ttcaacctat
     6121 ccatacaaat cagtaatccg aaaatataca tcccagatag cacaaggggc aaacgtccta
     6181 taatgagaga cgaccctgaa gaaaaatcga ttattcgctc aaataagatt aatccctctt
     6241 ctatcagcaa aagtaaagtt tccggcagcc agcgattgaa gagtgtgaga aataacacac
     6301 caggaagaaa aagaaacttg aataccggtg caatcagtaa tgtaaaaaga ctacctaaaa
     6361 taggccactc ataaaaatgc catacaatca acggcatcgt gatcatagta aacaggacgt
     6421 gagaggccgt ttttttcaac caatctccat caaaagaaat caggataaac aaaaaactca
     6481 tgaaaaaact aagctgtcca cccgcatgaa aaagaaatcg aggatcaaat agtaaaagaa
     6541 gccaaaagac aactgcaaat aagtccatgg tggaataatg cttattcagc aatttgacta
     6601 gcaatcgcaa taaaaacagc aaactagcac gcaaaacaga aatagaactt cctgcaatag
     6661 aaacataaca gatagttagt agactcaacg gaataatact gacgtgtagt ggccatccga
     6721 tacgtcgaaa taaataatac agccacccta aataaaattg gatgtgtaga cccgatagac
     6781 tgaacagatg cagcaaacca ctctccctaa aaatttcttc tggttctctg aatcctgcat
     6841 ctttatagcc ccataataaa gaattaatat atacacttgt tttttgcgga aattcagatt
     6901 ggacaaagga gataaaagat cctctttttc tctgaagcca gtttcgccag tgattttgca
     6961 tggtgatctg ttcaatcttt tgaatttgga accgtccaga aattctatgg ttcctgaagt
     7021 aagcttcttg gtcaaaatta taaagattcc gcttttttgc tggagagatg aactcgcctt
     7081 cagcagtcaa ttctattccc tttcctcgga agttaagcca tgttttcaat tctgcttctg
     7141 tgtttacttg ataagaaagc tggagctttt gtttattctc tgataacctt ccttccattg
     7201 tcaaaaaatt accatttgca cgaatcgtat ctgtaaatac agtaatttgc gcagatgttg
     7261 ttttttcctc taaaaatgaa tactcttgta gcctataaga aatggatacc gttatgctac
     7321 acaaaataca caagtacgcg ataaaacgtc cttcttttag cgaaaaccat aaaaaaattc
     7381 cggcaagaag caaacgataa ccccaattgt cagtgtaata caacaaacag ataatcatcg
     7441 acgtaattgc cggaaaaatc aaccgatttc gaacatgcct ttctaaaacg ctagacatgt
     7501 tttcctgtta attcattttg aagttttgaa aaatgctgtg agtctaaagc aacttggtgt
     7561 acagatacac ctaattgttc gatcaacgct tgggcataag gatcatttcg atagtcatgg
     7621 agataattga tccctttcac accagcttgc aacaaagcct ttgtacaggc caaacatgga
     7681 aaatgtgtga catagatttc tgaaccttcc gtcgggatac caagttttgc acactgtagg
     7741 agtgcattca tctctgcatg gatcgttcgc aagcagtgtc catccacgac gtaacaccct
     7801 tcatctatgc agtggacatc tccactgact gctccattat agccgccagc aatgatcctt
     7861 ttttctcgaa cgagtgtcgc tcctacttct aaacgtgtac acgtactacg taaagataac
     7921 agtactgcct gagccataaa gtactggtcc caaggaatgc gttcattatg tgacttatca
     7981 gtaacttttt ctttattcgt atcgcatgct tttgaagatt tttctaacat gttttatatt
     8041 cctccgtttt ttcaagttga ttaaaaagta ctcattgctt aaacaaaata ttctttaaat
     8101 ataacgaatc atcctgtctg acaaaagatc ctacccttca ctgccgaaat gaagtccgtc
     8161 atccattagc ccattataaa aattcttcga atccatttgt tcaagaaatt ccgtataaaa
     8221 atcgatactt cacaatcatg gcgttttgct acttctgtga gaacagagat atattctttg
     8281 atcaacttgt tattccgata tttttgtttt gtctcatcga ctgctggtgg cgtgataaag
     8341 aaagcatgat cagaactata gtcttttttc agctgaacta tcatccactg cagatttttt
     8401 ggtacgtttc tttagaaagt tgtttatgct ctgctagatc attcgtccca atgagaataa
     8461 atactttatc tgctgtatag ctaagtacat ctttggcaaa gcgttctcgt agagcttgac
     8521 tgttattgcc gctgacagcc gtattcataa tatgaatccc agatatttta ttttggagat
     8581 agacattcag cattggtttt tttaatgttt ctttgcgaac aatcaagcta tttcctatcc
     8641 aaaaatacta cctcctcgtc ctgccaagct cttagaattg gattattctt agtatccatt
     8701 agcgtccatt tattgctcgt gccagtcgat tttgaatcat tctatttaat tgtaacgaag
     8761 ttttttagtt tttcaactgt cttttcaccg ataccagaaa tctcctgcag gtcttccact
     8821 ttctggaagg atccgttttc ttctctaaat cgaatgattt cttgcgcttt gactgatccg
     8881 atcccaggaa tctgctgaag ttctgatgga gtggctgtat tgatatttat tttgggaggc
     8941 tcggaatctg ctgtaggctg ttttccttcc gaggatgcca actgatcaag atgttctggc
     9001 agctcttcac ctacagcagg gatatatagg acttgctgat cttctatctt cgcagaaaag
     9061 ttgattcgat cgctatccgc ttcttctaat aatccacctg cacgctttag tacatcatag
     9121 acccgttctt ctgaagaaaa ggaatagata ccaggctcct tcacacttcc tttgatatcg
     9181 gtgtacatga cctttggacg agcatcctct tctacatcgt ttccttcagc tgatggatct
     9241 tctccactgg aactaaacga agaaagtatt tcttttgcag cagtctcttc tgtttctcta
     9301 taaaatagca aatttgataa taaaaaacca cttgcgatac aaaagataag aactatcata
     9361 gacatccaaa tgatcaattt gggtgaagct agtattttct tgctgatttt ttctatcatt
     9421 gttctttttt c
//
LOCUS       DAZKCN010000099         9391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000099 DAZKCN010000000
VERSION     DAZKCN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.102"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..790)
                     /locus_tag="ACG8K3_002251"
     CDS             complement(122..790)
                     /locus_tag="ACG8K3_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9146078.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            924..1211
                     /locus_tag="ACG8K3_002252"
     CDS             924..1211
                     /locus_tag="ACG8K3_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146079.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1627..3165
                     /locus_tag="ACG8K3_002253"
     CDS             1627..3165
                     /locus_tag="ACG8K3_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP9146080.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3162..4127
                     /locus_tag="ACG8K3_002254"
     CDS             3162..4127
                     /locus_tag="ACG8K3_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146081.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4114..5436
                     /locus_tag="ACG8K3_002255"
     CDS             4114..5436
                     /locus_tag="ACG8K3_002255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9146082.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5499..5603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5677..6420
                     /locus_tag="ACG8K3_002256"
     CDS             5677..6420
                     /locus_tag="ACG8K3_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9146083.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAV
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6435..7427
                     /locus_tag="ACG8K3_002257"
     CDS             6435..7427
                     /locus_tag="ACG8K3_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9146084.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7439..8176
                     /locus_tag="ACG8K3_002258"
     CDS             7439..8176
                     /locus_tag="ACG8K3_002258"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146085.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8166..8888
                     /locus_tag="ACG8K3_002259"
     CDS             8166..8888
                     /locus_tag="ACG8K3_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP9146086.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8972..>9391)
                     /locus_tag="ACG8K3_002260"
     CDS             complement(8972..>9391)
                     /locus_tag="ACG8K3_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9146087.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3078 a         1610 c         1976 g         2727 t
ORIGIN      
        1 ttttcgcaga tcgtttgcaa aaatagttta tttttttata aagaagattt tactgaataa
       61 tatcatttta gtttacataa tgatatttct gtaaattaaa taaagtagtg aaaaataaga
      121 atcagtgctc taactgttca agaggatcaa gtggttcaaa tgtttctgta gtctgttttg
      181 cttccaattg gggcaaaaca gatccaattt ccagatactt cactttattc aatgtataag
      241 atagaataga acagctaagt aatgtataca gaaaatgtgt gagatccaaa taaagcaagg
      301 agacaactaa aataccaaaa gtaatcaaga aaagactttt gccaatctta atatgaaaat
      361 atttttcaat ggcttgataa atgatatctg ttccgcctgt gcttccgccg aatcgaaaga
      421 cgagaccgca accaatgcca gaaagaatac ctgtcatcaa actattgatc agtagactat
      481 ctacagcaaa atgtatatga aggctctccc ataaatgtaa aaagaaaact agcgcaaatg
      541 ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata
      601 aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa
      661 gcaaagcaat accagtaacc ccaccatctg ccagcttaga tggaatattg attcctgtca
      721 cagctaatcc gtagattcct gcgcctaaaa tgattccgca caattctgta aataaattta
      781 attttttcat ttctagcctc ttttcttttg aaaattttca gcaacttctt tattatactg
      841 acttatactc ttacttcaag aggaaaatga aaaaaaatta tttttggctg tttcttttta
      901 tttatcaaat aaggagtgga aaaatgcgag cagcagactt attgacaatt cttacaaaat
      961 cacccaaaaa aacagagtat caaggagttt ttttggctca agaacaaaaa ctggtgcctg
     1021 tgacaatggt ccagataaat aaagcaaatc aatttatctt tttatttgaa cacggcaaag
     1081 caccacttgc tatgaaagac atctttcttt ttttgatgaa gaaccgtgag aaagctgttt
     1141 attatcagtc aaaagaagaa ctcattcctc tttatggagt gaaagagata gaaaataaag
     1201 tggtgatata agtaaaaata agaaaacaag acgtttatga tgtcaaaaca accatttttt
     1261 acatttttgg caaaaaagtg tcatgataca gtataatatt cgttgaacag gccaataaag
     1321 gtgttctccc ccacgagatt tctactttat tggctgtttc tttttattaa aataaaagtt
     1381 gtgtgtgtaa aaaagatata gctgattcca agtgacaatt tcgtatagat ttttatggta
     1441 ttcgtagtga aaaaacgaga accaaaaagt tgatccgact ttgttgcttg gattcttttt
     1501 ttgtcagcat atttagaggt atttttttct tttttttgtt cgtgctgtaa ggttaaggca
     1561 agacaagtta gttgggaaaa aggaaagaaa tgtttatact taaagaataa cagaaaggaa
     1621 ggtcgcatga ataattttat acaaacatta agtgaaagaa aaggcgagtt gctggaagct
     1681 gcatttcaac atctgtccat ttctctcatt tctttgctga ttgcagcact gattgctatt
     1741 cctttagcga tttgggcagc caatcataaa aaaatggcag aagtcttatt gcagatcacg
     1801 ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt tacttatccc atttgtaggg
     1861 atcggaactg ttccagcatt gattgcactt gttatttacg ctttgcttcc gattttccaa
     1921 aacacgtata ttggtctagc tgaaattgac ccgtctattg aagaagcagc ggtagcattt
     1981 ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt
     2041 atctcaggta tccgaactgc cttagtttta atcatcggta cggctacttt ggcagcattg
     2101 atcggagcag gtggattagg gacatttatt ttgttaggta ttgaccgaaa tacacctgtt
     2161 ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag cggactgatt
     2221 cgctggttgc agcataagaa acctcgatat tccatcatta ctttagcagc agtggtgtgt
     2281 ttatttggcg gcgtttcttt atatcagtct gccttgttta aaaatgaaac catcacgatt
     2341 gctggaaaat taggagcgga acctgaaatc ctgatccaaa tgtataaaga attgattgaa
     2401 gacaacacaa aaacagaagt tgaattaaaa ccgaattttg gcaagacgag ttttttgttc
     2461 aatgcactgg atcaaggtca aatcgatatc tatcctgagt tcacaggtac tgtcctagaa
     2521 agcttggtga aggtacctga agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat
     2581 gacgaagcag aagaattatt gaataagcaa ttcgatatga cattattaaa accaatggct
     2641 taccaaaata cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa
     2701 aagatttccg atttggcagc tatctctgat cagatcaaag cgggattcac actggaattt
     2761 attgatcgtt cagatggata taaaggaatc caagaagcat atggcttgaa tttttcaagt
     2821 gtacaaagca tggagccaag tttgcgttac caagcaatca ataatggaga tgtcaatatt
     2881 gtggatgcct attccacaga tagccagctc aaggagtacg atctagtgac tctagaagat
     2941 gatcgagggt tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa
     3001 gaacatcctg aaatcgttaa agcactgaat aaattaagcg gaaaaataac agaggatcaa
     3061 atgattgata tgaattacca agtcaatgtc gaaaagaaaa agccgacaga agtagcacga
     3121 atcttcctga aaaatgaagg attagtaaag gaggaagatc agtgactaaa ttaatcgagt
     3181 tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta aatctggcaa
     3241 tcaaaaaagg agaaatcttt gttttggttg gtccttccgg tagtggaaaa acaactactt
     3301 tgaaaatgat caatggatta tctaaaccat cagcaggaga catctacttc aaaggaaaat
     3361 cgttaaatga atataatctg cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa
     3421 tcgctttgtt cccgaccatg acagtcaagc aaaatattga agtcatccct gaaatgttag
     3481 gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa gttggcttat
     3541 ctcctgatat ttatcgagat cgtatgccta gagagttgtc tggaggagaa cagcaacgga
     3601 tcgggatcat ccgagctatt gctgcaagcc cagatgtgat tttgatggac gagccattta
     3661 gcgcattaga tccgatttca agaaactcat tacaagaatt ggttttgtcc ttacacgaag
     3721 aattgggcac gaccatcgtt ttcgttacgc acaatatgga agaagcaatc aagctaggtg
     3781 atcgtattgc atttatgaag gatggagaaa ttatccaatg tgacacacca gagcagttat
     3841 tgatgaatcc taaaaacgac tacgttcggc atttctttga tgaaccgaaa cagacgaaag
     3901 agtggcgagt agaagattta gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg
     3961 ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt atattcgctt ctttcggtgt
     4021 atccaagcat tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa
     4081 tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa catatgatgc aatcatcatc
     4141 ggttcaggtg tgagcggctt aagtgctgct tatggattga aagaagcagg taaaacagta
     4201 ctagtggtcg aagaagatct atggggcggg acttgtccta accgtggatg tgatcctaaa
     4261 aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa aacaattgag tggaaaaggt
     4321 tttaacgaga tcccaactgc caactgggaa gaacttcaaa aattcaaacg aacatttacg
     4381 gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca
     4441 ggaaccgctc gttttttgga tgattcatct attgaagtca acgaagaagt cttccatgcg
     4501 gattacttag ttttggcaac cgggcaaaga cctacgatct taccagtgga gggaaaggaa
     4561 tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga aatcattttt
     4621 atcggtggtg gttatattgc ttttgagcta gctacgattg ccaatgctgc agggagcaaa
     4681 gtaaccattg tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag
     4741 gaagccgtcc atcaaatgga agcatctggc attcagtttg cttttggtgt tgaaacccaa
     4801 aagatcattt cagaaggtac tcgctatcgt ttagtcggaa aagagacgga attagtagca
     4861 gatatgattt tttgtgcgac tgggagacag ccgaatacag aatctttagc acttgagcaa
     4921 gcgaatatcg tctttgataa acacggaatt gcagtcaatg attatctgca aacgagcaat
     4981 ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc
     5041 gccacttttg aaggaaatta tgtagctaag cggataacgg atgcaacaag cgaacctatc
     5101 aaatatccga ttatacctac gatcgtctac gccagtccta aacttgctga agtaggagtt
     5161 acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga tggatcttac cagttggttc
     5221 acctatcatc gtgtcaatga accagttgct aaggctgaat tgacctttga ccagcaaaat
     5281 tatttgattg gtgctgcagt gattagcgag caagcagacg aattgatcga tgatttgaca
     5341 ctcgtcatca atcaaaaatt gactaaaaaa gagttggata gctatatcat ggggtatccg
     5401 acattggcaa gtgatctctc ctatttattg aaataatcag catgataact aaagcgcttg
     5461 ttgctttttc cttcttgtgc tacactttaa aaaatgaata cctgtttctg gggatgtcgt
     5521 agacgacatg agagtaagtt gtgaacttag acccattgaa cctgtcagtt aatactggtg
     5581 aagggagaaa catagcagac gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt
     5641 ttttgttatt catattagaa gaaaggaggt aacaaaatga agaaaaatct ttatccagca
     5701 gtcagtatgg cattcatttt aattatttgg cagattgttg tcatagtttg gaaaatacct
     5761 gcctttattt tacccggacc tgctgctgta ttttctgcat tagcggtaga tgcacaagta
     5821 ttactttccc attctgtcac gacattaaat gaagcagtgc ttggtttggt aatagctagc
     5881 gttctttctt ttattactgc cttggcaatg gattattgga agtttttaga aatgtctttt
     5941 tatccgttac tagtgatttc acaaatgttg ccaatcatgg tgttagggcc attacttacc
     6001 ctatggtttg gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat aagctatttt
     6061 ccgatcgtag tttctttttc ggatacattg aggaaagtat caaaagagca aatcatcttt
     6121 ttgaaaacga tgggggcaga tacagggaag atttatcgaa tatataaaat accagcgggt
     6181 ttatccggct tcttttccgg tttaaaagtg gctgctactt attgtgtcag cggagcagtc
     6241 gttggagaat ggctaagcgc tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac
     6301 agctaccaga tcgataaagt ttttgctgcg atcatttgtg tcattctatt gagtttattg
     6361 ctgaatggcg cttgttcatt attgaaaaaa gggtattatc gtatgcttta tattttttaa
     6421 gagaagggga tcaaatgaaa aaattatggc ttttattacc gctactgttg cttttatccg
     6481 cttgtggcac tgcaaaagaa acaagtagca aacaagaaat aaaggatttg aaaaaagtga
     6541 cacttgtttt ggattatgta ccgaatacta atcatactgg catctaccta gcaaaagaaa
     6601 aaggctatta taaagaggct ggtttaaatg tccaaatcat cgaaccagga gataattcga
     6661 caagtattgg tctagtaggt gcggacaaag cacaatttgg tgtaagttat caagaagatg
     6721 tcacttatgc tcacgcagat ggacagaata taccagtcaa agcaattgct acagtgatca
     6781 aacataatac ttctggattc gccaccctat cagacagtaa catccattca ccaaaagatt
     6841 ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg
     6901 tgatggaaaa agacggcggg gacttttcaa aactgacaat ggtaggaagc aatggagaag
     6961 ggccagaaag cttaggaaaa tcctcagata tccaatggta ctttgaaggt tgggatatga
     7021 tcaaagcaaa agaagcgggt attgaactaa actatatccc attgaaagaa ttagatgaac
     7081 ggttagatta ctatactccg gtaattatta cgaatgatca actgatcaaa agtgatccag
     7141 aattggtaca gtcttttatg gatgccacga aaaaaggcta tcaggaagct attaaggatc
     7201 caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag
     7261 aagaatcaca agcattcttg tcaaaaaatt atacggatga tcctgagaat tggggattga
     7321 tggaagaaaa agtttggcgc aattatacag cttttatgca ggaaaacgga ttgatcaagc
     7381 aagatgtacc ttcgcaagaa ctattcacta atcagtttat aaaataggag tgatagatat
     7441 gtcaatccaa atcagccact tgaatgtagt cattgaaaac ctgaaaatat tagaagacat
     7501 ccatttcact atccctaaag atacctttac atcgattgtc gcgccaagcg gagccggtaa
     7561 gtcaacattg ctgaaaactt taacaggagt ccagcctatg acttctggaa cgatcaaagt
     7621 agacgatcaa aaagtaactg gtctccatac agcatttagt tatatgccgc aagaagatat
     7681 gttacttcct tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct
     7741 aacaatcaat gaagaacaag taagggatta tctggatatc tttgggttaa acggctatga
     7801 acattttttg ccggaacaat tatctggcgg gatgaagcag cgaacggcct tgttacgaac
     7861 aatcatgaat ccttcttctt acttgcttct tgatgaacca tttggagcgt tagatgctat
     7921 gaccagaggg cagatgcaag actggatgtt gaagctgcct aaaaaagcaa agcggactac
     7981 tttacttgtg acgcatgata tcgaagaagc aatttattta tcagacagga ttcttgtgct
     8041 ttccgcccgt ccggcacatg tgattgctga gattcaagtg cctgaaaaac gacggtcacg
     8101 tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt tatcagttat tggcaggtga
     8161 attagatgtt aagtgatgct cattgtcatt tagatgggag tcagagttta gcactccttc
     8221 agcaggaaca cacgattttg acgatcatca attgtgattc gccagaagaa tggaaggaaa
     8281 acagacagct tgcagcaagt aagactcaag ccttaagtta cggtatccat ccttggaaag
     8341 cagatagcta tacgtttgaa caagttgagc cttttttgaa aaaagcaagg atcatcggtg
     8401 aaatagggtt agataatatt tggacgaatg taccgatgac tacacaaaaa aaagtatttg
     8461 agcgacagct tgcttttgca gccataaatg agaaacctgt tgtactgcac acaaaaggat
     8521 gcgaaaaaga gatcttaaat tatatccaag cttaccctaa tcactatttg atccattggt
     8581 attcttcttt ggactatcaa caagactata ttgatttagg ctgttatttc agcattggtc
     8641 ttgatttgaa aaagaatcca gcagtctggc aactagctaa agcagtccca atcgaccact
     8701 tacttattga aacggatgga ctacatgcag cagagtgggc tttagggggg attttttcag
     8761 aaaaagatta tgtctcactg cttgaagaac agttaaagac aatcgctgag atcaaagagc
     8821 tatcagttga agaagttaaa aaaaatactt atcaaaactt ggaacgtttc attagattag
     8881 gtaaataaaa aagagatgtg acacaactag tgtcgcacct ctttttggct ttctttgtat
     8941 aaaagtaaac tgtttgatta ataaatgaat cttagtacca accgtttgct tcccagaatg
     9001 ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct actctttctt
     9061 ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa cgtccgatgt
     9121 aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc cattcttttg
     9181 cagagcttgt gcttgctgga gtagcagcag tttctgttac agcaggagca ggttgtgttt
     9241 cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca ggttgttcag
     9301 cttggactgg tgcttcttga acggctgctt gaggttcagc agctgcttgt gcagagcctt
     9361 ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DAZKCN010000100         9286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000100 DAZKCN010000000
VERSION     DAZKCN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.103"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8K3_002261"
     CDS             <1..435
                     /locus_tag="ACG8K3_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP9146088.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8K3_002262"
     tRNA            complement(759..842)
                     /locus_tag="ACG8K3_002262"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8K3_002263"
     CDS             1370..1870
                     /locus_tag="ACG8K3_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146089.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8K3_002264"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8K3_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP9146090.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8K3_002265"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8K3_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9146091.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8K3_002266"
     CDS             3665..4156
                     /locus_tag="ACG8K3_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP9146092.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8K3_002267"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8K3_002267"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9146093.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG8K3_002268"
     CDS             6881..7507
                     /locus_tag="ACG8K3_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9146094.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG8K3_002269"
     CDS             7530..8234
                     /locus_tag="ACG8K3_002269"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9146095.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG8K3_002270"
     CDS             8426..9259
                     /locus_tag="ACG8K3_002270"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9146096.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2829 a         1791 c         1652 g         3014 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttg
//
LOCUS       DAZKCN010000101         9174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000101 DAZKCN010000000
VERSION     DAZKCN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.104"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..1451
                     /locus_tag="ACG8K3_002271"
     CDS             27..1451
                     /locus_tag="ACG8K3_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP9146097.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1632..2405)
                     /locus_tag="ACG8K3_002272"
     CDS             complement(1632..2405)
                     /locus_tag="ACG8K3_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9146098.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2402..3307)
                     /locus_tag="ACG8K3_002273"
     CDS             complement(2402..3307)
                     /locus_tag="ACG8K3_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146099.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3461..3781)
                     /locus_tag="ACG8K3_002274"
     CDS             complement(3461..3781)
                     /locus_tag="ACG8K3_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP9146100.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4255..4941
                     /locus_tag="ACG8K3_002275"
     CDS             4255..4941
                     /locus_tag="ACG8K3_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP9146101.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4943..5887
                     /locus_tag="ACG8K3_002276"
     CDS             4943..5887
                     /locus_tag="ACG8K3_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP9146102.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            6086..6730
                     /gene="pcp"
                     /locus_tag="ACG8K3_002277"
     CDS             6086..6730
                     /gene="pcp"
                     /locus_tag="ACG8K3_002277"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP9146103.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            6809..8086
                     /locus_tag="ACG8K3_002278"
     CDS             6809..8086
                     /locus_tag="ACG8K3_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146104.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(8270..9019)
                     /gene="pnuC"
                     /locus_tag="ACG8K3_002279"
     CDS             complement(8270..9019)
                     /gene="pnuC"
                     /locus_tag="ACG8K3_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9146105.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2884 a         1636 c         1890 g         2764 t
ORIGIN      
        1 gcggttttat tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt
       61 taattccact tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta
      121 tgacggacat ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa
      181 cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa
      241 aaggcgggat ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg
      301 gttggggtta ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga
      361 gcgcattagg atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga
      421 tgagtacagt ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta
      481 gtttcgtcaa tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag
      541 tagctatcag cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat
      601 tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga
      661 tgctttgggt atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac
      721 aatcagatgt tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct
      781 tggtttccat gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc
      841 agcctgctat gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca
      901 acggcggttt gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg
      961 aaacaatggg aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca
     1021 aaaatggggc accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac
     1081 tttcattatt attcactgac agtgcttata actttgctta cacacttggt acagcagcta
     1141 ttttcttcac gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag
     1201 aatgggtcca atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag
     1261 tcgtagcctt taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt
     1321 acctcaaagc acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt
     1381 tacttatagg tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc
     1441 ttcacgtata gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa
     1501 aaacatacta ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa
     1561 cggtgtttaa gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt
     1621 ttgatgagtc atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa
     1681 acgatgtaag ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc
     1741 atgatctctt cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg
     1801 atccactccc atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa
     1861 taaaagacga ttcctgctga gatggatgca ccggaactat tgataaagca agcaatcatc
     1921 aacactaaac ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag
     1981 ctttggatca gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga
     2041 atcagtttca tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata
     2101 actaatgtga tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga
     2161 tttttcaatg taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg
     2221 ataaagacaa tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg
     2281 atcgtcgtgt attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca
     2341 gataaaacag cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat
     2401 gtcatttttc ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat
     2461 cattcgtcag atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca
     2521 cggttcctaa taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag
     2581 tatatccagc ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga
     2641 tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt
     2701 gattgatcag gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga
     2761 tcaagaaaga ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg
     2821 cttgtggatc caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta
     2881 aggcaagagc aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt
     2941 gaatgtactt ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg
     3001 tttcagagaa taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt
     3061 cgatcaatgc tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac
     3121 caatacagat tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg
     3181 tttttccagc gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac
     3241 tgacatcgct gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac
     3301 ttttcaaaaa tattcactcc ttttctatag atagtacaag catacacctg atgagtggag
     3361 aagtcgctag gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata
     3421 aaaaggctta ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct
     3481 tgatgccaat gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct
     3541 tatcatgaaa caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga
     3601 taggataaga gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag
     3661 caatcatgcc aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt
     3721 tcatcatcgt tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca
     3781 tacttacatt cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttaaa
     3841 agacctaatg gaatgatcat cctctttatc ctacccggtg gcagatcgta tatctttttc
     3901 tgtagtatag cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca
     3961 agcactgacg gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa
     4021 gaagttgtta tttattaatc atttaaaaaa aacagtaact agaccttatt tctatatatt
     4081 atcgggcaat aaattaaaaa aagctttacc ttacaataag taagataatt tttgtgataa
     4141 aaaaataacg aaaaatattt aaaaatagtg tctatttcgt gagaaaacaa ctatattttt
     4201 tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa atatatggaa
     4261 aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt taaattagac
     4321 tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg tcttggcatt
     4381 caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat ggcgattttt
     4441 atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga atcagcggct
     4501 tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat ttatgggatc
     4561 tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg atttgttcgt
     4621 ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga agtccatcct
     4681 aagcatgaag aagaactaaa aggtatggct tcagctatgg aaaatgtctg ctggttcttt
     4741 ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac actgaaagat
     4801 ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt ggctatcacg
     4861 gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc gaagaaatat
     4921 tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt tattaggcga
     4981 taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca ctggagtaag
     5041 aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct ggacggtctt
     5101 aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg gcttgatctt
     5161 tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc ggcttccatc
     5221 aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta ttccagcttt
     5281 gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg ctttagtcgg
     5341 cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc gagataatcg
     5401 accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc cgttaagcat
     5461 gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag ttgggacagt
     5521 gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg gcatcatcat
     5581 ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg cggccatcac
     5641 cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga atccagcgtt
     5701 aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac cgatggctgc
     5761 taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg gagtgatcaa
     5821 aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata tgattctatt
     5881 taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc taacattgta
     5941 gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt atttaaacta
     6001 ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa ccatatgtta
     6061 aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg atccatttgg
     6121 tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg aaatcagcgg
     6181 tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc aagtagtcaa
     6241 agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag caggtgggcg
     6301 gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa tccctgataa
     6361 tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg cttactttac
     6421 ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac cagcgtacat
     6481 ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc ttcatttgat
     6541 cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct cacccgatca
     6601 agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg cttcttcttt
     6661 gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa atgccggaac
     6721 aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag tagccagctt
     6781 tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct ttgaagaatg
     6841 gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat acacaagcag
     6901 tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag tcgataagca
     6961 ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg caacagattg
     7021 ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc agcaaaaatt
     7081 gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc caaaaaccgg
     7141 aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct ctccgacagt
     7201 ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg aattattatg
     7261 gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact gttttgtctt
     7321 gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag ctcagcggct
     7381 cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag cattggaaaa
     7441 tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac agataaaaaa
     7501 agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat tcttgatcaa
     7561 ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag tgccagtaca
     7621 tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac gtcaacgaac
     7681 accagaagtc actgctcaga tggaatattt tgcgaaaaac caacgattca tccaaccaaa
     7741 aatctatcta tttatttgga agcaatattg tcgctatggt tttacagaag ggttcttcga
     7801 cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag aacgagaagc
     7861 tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc aactagtagt
     7921 gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag gaaaacatta
     7981 tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag aagaatcact
     8041 gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa tagaggctgg
     8101 gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct tttcaaattt
     8161 ttgtgaattt cagcttattt ccgaaggagt tgcttctgat tccgccgttt atacgtgttt
     8221 agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tcagtttttt tatgcgtgtt
     8281 gttgtgcgtt gtagtattct tcttccattt tttgcatacg ttcacgttct ttttggttat
     8341 accaaggtga aacggtaaat gcaaggacat cattcagcaa gtaaacagag ctgttcacga
     8401 acatcgccaa acttgcagat ccatgcgtaa atgaaatata ccaaaggacc atttgagcaa
     8461 gccctgaagc taaccaccag taatattggt tgttgtaacg caagaaactg atgataccag
     8521 cagtcaagct aatagaaaaa gcaatggcat ctacccacgg ccgaggatca tctgtataac
     8581 gaccaatcag gaatcctgaa acaagataga caaggaaagt actgatgatt gcgataaacc
     8641 agctttttcc agtgaatttt cggatttttg aagccatatt atgattccat tctttactta
     8701 acaatacggg gatatccaat gtgatcatat aagcaatctg ttcaccgata cttaaatagt
     8761 tctttgctga aaacccaacg ataataaaac aaatcgcaga caaaatacct aaaataccgt
     8821 tgactgattt tgccgcattg atggataaaa tacacaatac accaagcgtg gtaccgataa
     8881 atgtaataac ggacagccaa gtgatgggac tattgacaag agtcatgacc tggcagccaa
     8941 gactgaaaaa gaaaagataa taattttgtt gcggccagcc ttttagctga tgagctaaaa
     9001 actgaaaata tgacttcatg tatgaggact ccttaagaca ctaaatgtag tgtgttctgt
     9061 atttactaac acaatctatt gtatgctttt cttgaaaaaa aactagtctt tcaattataa
     9121 ataatttgtg ttatgataat tttctgtttg ttttcttgaa aatataattt ctta
//
LOCUS       DAZKCN010000102         8984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000102 DAZKCN010000000
VERSION     DAZKCN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.105"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8K3_002280"
     CDS             complement(15..1154)
                     /locus_tag="ACG8K3_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP9146106.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG8K3_002281"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG8K3_002281"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9146107.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG8K3_002282"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG8K3_002282"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9146108.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG8K3_002283"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG8K3_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9146109.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG8K3_002284"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG8K3_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9146110.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACG8K3_002285"
     CDS             complement(6168..7259)
                     /locus_tag="ACG8K3_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP9146111.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG8K3_002286"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG8K3_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9146112.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACG8K3_002287"
     tRNA            8802..8873
                     /locus_tag="ACG8K3_002287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2330 a         1895 c         1659 g         3100 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgtccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacactt gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc ttttgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttga tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc caccacctgt
     3901 tgagatgtgt gtgaatttgt cagcgaatcc taattgttct gctgctgcag ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttct actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat taagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattgggcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaaa cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga ggaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc cgacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaaatctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt ggaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagagaatt ggtggacagt tttttgttgt atctgtatag atat
//
LOCUS       DAZKCN010000103         8967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000103 DAZKCN010000000
VERSION     DAZKCN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.106"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..1187
                     /gene="gmd"
                     /locus_tag="ACG8K3_002288"
     CDS             141..1187
                     /gene="gmd"
                     /locus_tag="ACG8K3_002288"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9146113.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1195..2280
                     /locus_tag="ACG8K3_002289"
     CDS             1195..2280
                     /locus_tag="ACG8K3_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9146114.1"
                     /translation="MNKHICFYLSSHGFGHIARNLPIIMATLCRCEAKITLVCGANHL
                     DFAKRNLEQELPKELIDRVRYRPINTDIGLINREGTLLVDTGKLTEICEYFLAKVPSL
                     AKTEAEWLKREAVSAVLCDMPIWAMDACDMAGVPFLYVGNFTWTELYREFLPEHIWKE
                     YAKRYGKIQHTMLYALNNEEMLEFLQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVF
                     VALGMSARFKQPIDVSHAPYDFITTLGVPLVGENVTVISNSTPNTQDYIAAADYVITK
                     AGWGTVAECILAQRPMALFARDTVLEDRTTICKLQKLGLAISVTPENLHRVERVIEDL
                     KQLDTSNFDTFYDAASEIADKLISLMQ"
     gene            2317..3261
                     /locus_tag="ACG8K3_002290"
     CDS             2317..3261
                     /locus_tag="ACG8K3_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFP9146115.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3329..4642
                     /locus_tag="ACG8K3_002291"
     CDS             3329..4642
                     /locus_tag="ACG8K3_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFP9146116.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4834..5628
                     /locus_tag="ACG8K3_002292"
     CDS             4834..5628
                     /locus_tag="ACG8K3_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HFP9146117.1"
                     /translation="MTLTEVLQEHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPT
                     DEYAIEKENGQLGKTEMWYVLDASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKK
                     NDVFFIEAGTIHAIGAGALIAEIQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVAN
                     LKGLSEPKQPLRVLKYRAGVAAELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVL
                     LCVNGCGTVRFDNESLDIYKGDCLFVPADSTEISIHGSIQFLDVRG"
     gene            5633..7285
                     /locus_tag="ACG8K3_002293"
     CDS             5633..7285
                     /locus_tag="ACG8K3_002293"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP9146118.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7309..8175
                     /locus_tag="ACG8K3_002294"
     CDS             7309..8175
                     /locus_tag="ACG8K3_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9146119.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8217..8408)
                     /locus_tag="ACG8K3_002295"
     CDS             complement(8217..8408)
                     /locus_tag="ACG8K3_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146120.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8567..>8967
                     /locus_tag="ACG8K3_002296"
     CDS             8567..>8967
                     /locus_tag="ACG8K3_002296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146121.1"
                     /translation="MEEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSN
                     LWFRQEISIAEIEDAVLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKN
                     ISYLKVYTATPPKKPTPPRFQSNGWSLFFSH"
BASE COUNT         2432 a         1869 c         2273 g         2393 t
ORIGIN      
        1 cacgaaaaac taatattgtg ctaatgcgtg cgtagattgt tctatgaaat atcgaaaatt
       61 atcgtgtatt cttgaaaagt attagaaatt acgagccatt tatatggcct tatcacttac
      121 ataggaaagg ataatgtatc atgaaaaaag cgctgattac tggtattact ggtcaggacg
      181 gttcctatct ggcagatttc ctgctagaga agggctacga agttcacggt atcactcgcc
      241 gtgcttctat ttccaatacc gctcgcattg accatttgat gggcaagatc acccttcatg
      301 acggtgacct gtccgactca tcttctctga ttcgtatcat cagcattgtt cagcctgatg
      361 aaatctataa cctcgctgca cagagccatg ttcaggtttc ttttgatgtt cctgagtact
      421 ccggcgatgt tgatgctttg ggcgttatgc gtattctgga agcttgccgt attctgggcc
      481 tgacgaagaa gactaagatt tatcaggcat ctacttctga gctgtatggc aaggttgaag
      541 aagttcctca gcgtgaaacc actccgtttc atccttacag cccttatgct gttgcaaagc
      601 agtatggctt ctggatcact aaggaatatc gtgaagctta tggcatgttc gctgtcaacg
      661 gtattctgtt caaccatgaa tccgaacgtc gcggtgagaa tttcgtcacc cgtaagatca
      721 ccctcgctgc cggccgcatt gcagaaggcc tgcaggacca tctggaactg ggcaacatgg
      781 actccctgcg tgactggggc tatgcaaagg actatgttga gtgcatgtgg atgattctcc
      841 agcatgagac tcccgaggac ttcgtgatcg ctaccggcga gcagcacact gttcgtgact
      901 tcaccgagaa ggcattcgca gcaaatggca tgaccattcg ttgggaaggc actggcattg
      961 atgaaaaggg ctacgatgtt gaaaccggca agatgctcgt ttgtgtaaat ccacagtggt
     1021 tccgtccgac tgacgttgac aacctgtggg gcgacccgac taaggcaaag accgttctcg
     1081 gttggaatcc gcagaagacc tcttacgagg aactggttaa catcatggca aagcatgacc
     1141 gtgaattggc aaagcgcgaa gctgcaatga agaacgcagt caagtaagct gacattgaat
     1201 aaacatatct gtttctatct ttccagtcac ggcttcggac atatcgcgcg gaatctgccc
     1261 attattatgg caacactctg ccgatgcgaa gcgaagatta cactggtttg cggcgcaaat
     1321 catctggatt ttgcaaagcg caatttggag caggaactgc ccaaagagct gatagaccgc
     1381 gtgcgctatc gtccaataaa cacagatatc ggtctgatta accgtgaggg caccctactg
     1441 gtggacacgg gtaagctgac cgagatctgt gaatattttt tggcaaaggt tccttctttg
     1501 gcaaagacag aagccgaatg gctcaagcga gaagctgtca gcgcagtgct ttgcgatatg
     1561 ccaatctggg caatggatgc ctgcgatatg gcaggagtac ctttcctgta tgtaggaaac
     1621 ttcacttgga cagagctgta tcgcgaattt ttacccgaac atatttggaa agaatatgcc
     1681 aagcgttacg gtaaaattca gcacaccatg ctttatgcgc taaataacga agaaatgttg
     1741 gaatttctcc aaaacagcga aaaaacaccg acttccgttg tggcgcgtcc gattcataac
     1801 gacgaagtgc aacgcattaa atcacagcat gaaaaaccgc ctgtatttgt cgcactcggt
     1861 atgtccgcgc ggttcaaaca gccgattgat gtcagtcacg cgccgtatga tttcattaca
     1921 acgctaggtg ttccgcttgt gggagaaaac gtaaccgtta tttctaacag cacccccaat
     1981 acacaggact atatagcggc ggcagattac gttattacaa aggctggatg gggaactgtt
     2041 gcggagtgta ttctcgcaca gcgtccgatg gcactgttcg cccgcgatac agttttggaa
     2101 gatagaacaa ctatttgtaa attgcaaaaa ctgggtctgg caatttccgt cacaccggaa
     2161 aacctccacc gcgtagagcg ggtgattgag gacctcaaac agctagacac ctcaaatttt
     2221 gatacatttt atgatgctgc gagcgaaatc gcagacaaat tgatttctct catgcaatga
     2281 gagatgctct aaatttagaa aggagcaagc attattatga tggaaaaaga cgcaaaaatc
     2341 tacgtagccg gtcatcgtgg catggctggt tccgcaatcg tgcgcgagct gaaccgtcag
     2401 gactacaaca atattatcac ccgcacccat aaggagcttg acctgtgccg tcaggacgct
     2461 gttgaagcat ttttcgcaca ggaaaagccg gattatgtat ttctcgctgc tgcaaaggtt
     2521 ggcggtatta tcgcaaacca gaacgctctg gcagacttca tgtatgaaaa catgattctg
     2581 gaaatgaacg tcatcaactc cgcatgggag aatggctgta agaagttaca gttcctcggt
     2641 tcttcctgta tctatccgcg tatggcaccg cagccgatgc cggaaagctg tctgctgaca
     2701 agtgagctgg aaaagaccaa cgaggcttat gcactggcaa agatttctgg cctgaagtac
     2761 tgtgagtttc taaacaagca gtacggtact gactatatct ctgttatgcc tactaacctg
     2821 tatggaccta acgacaacta tcatcctact cacagtcatg tgctccccgc tttgattcgt
     2881 cgcttccatg aggctaagga agcaggtctg cctactgtta cctgctgggg tgatggttct
     2941 cctctgcgcg aattcctata tgttgacgat ctggcaaacc tgtgtgtgtt cctgatgaat
     3001 aactatagcg gtgacgagac tgttaatgca ggtactggca aggaactgtc tatcaaggaa
     3061 ctgaccgaga tggtagcaaa ggttattggt tacgaaggcg agattctgtg ggataccagc
     3121 aagccgaacg gtacacctcg taagctgctg gatgtttcca aggcaaccaa gctgggatgg
     3181 acatataaga ctgagttgga agatggcatc cgtctgtcct acgaggactt cctgaataac
     3241 ccgatgcgtg ctgaacgcta atttaccgag gttgctgtga tttttgagga ttgcagcagc
     3301 ctctttctta taaaaaggag agatcattat gaatattgtt ttactgtctg gcggttctgg
     3361 taagcgcctt tggccgctgt ctaatgacat ccgttccaag cagttcatta agattttcaa
     3421 aactgaagat ggatatgaat ccatggttca gcgtgtctac cgccagatta agaaggtaga
     3481 taacgacgca actgttacga ttgcaacctc taagacacag gtttctgcta tccataacca
     3541 acttggtgag gatgtgggtg tatctgtaga gccttgtcgc cgcgatacct tcccggcaat
     3601 cgcattggct accgcttatc tggtagatgt gatgggcatt gatccagaag agtccgtagt
     3661 tgtttgcccg gttgaccctt acgtagaaga tgactatttt gaggccctga aatctctgtc
     3721 cgatcaggct gacaaaggcg aagctaatct ggtgctcatg ggtattgaac cgacttatcc
     3781 ttccgagaag tacggctaca tcattcctga gagcgcagag cagactgctt tcgtaaatac
     3841 cttcaaggaa aaaccttctg aggctgtggc aaaggaatat atcgcacagg gtgcactgtg
     3901 gaacggtggt gtgtttgtct acaagctgaa gtatgtcatg gagcgtgcac atcagctgat
     3961 tgactttgta gattatcagg atctgttcaa caaatacgat actctgacta agatttcctt
     4021 cgactatgcc gttgttgagc atgaggacaa gattcaggta cagcgttttg ctggtatgtg
     4081 gaaggatctg gggacttgga atactctgac cgaaggtatg gaggaatcca ttatcggtaa
     4141 aggtgttatg aatgagaatt gcgatggcgt acacatcctg aacgaactgg atgttcctgt
     4201 gttggctatg ggtctgcatg atgtggtcat ctgtgcatct cgggaaggta ttctggtttc
     4261 cgataaggaa cagtccagct atattaagcc tttcgttgac aagttcgagc agcagatcat
     4321 gttcgcagag aagtcttggg gtagttataa ggttatggat gtagaagcag agagcatgac
     4381 cattaaggtt actctgaatg ctagtcactc catgaattat cacagccaca agaaccgtga
     4441 cgaggtgtgg gtggttctgg ccggtactgg taagaccatc gtggatggat atgagcaggt
     4501 tgttaaggct ggcgatgtga tcaccatgag tgctggctgt cggcatacca ttatggcaga
     4561 tactgagctg aagctgattg aagtacagct tggcaaggat atcaatgtta aagataaaca
     4621 gaaatttgag ttagagtatt aaaaaacgga ggagggctcg ttatacagaa accgttttta
     4681 ttgaagcctt ccgcaaagga ttatctgtgg gggggaaccg cctgaatgat gaatttgcaa
     4741 aaggaattga gatgtctcct cttgcggaaa cttggaatgc tctacgcacc cggatggacc
     4801 gagctttgtt gtgtctggtc agagtatcag gggatgacct tgacggaggt gttgcaagaa
     4861 cacccagagt ttctgggaac gcatccgcag accaaaggag aattgccgat cctgatcaaa
     4921 ttcattgatg cgaaaaagga tttgtctgtt caggtacatc ccacagatga atatgcaatt
     4981 gaaaaagaaa acgggcagct tggaaagaca gagatgtggt atgtactgga tgccagctta
     5041 atctatgggc tgcagaaaat aacagaagaa gctgtgatcc gtgaagcgat tgctcaggga
     5101 actgttgagc gttatcttca gaaggttccg gtcaaaaaga atgatgtgtt ctttattgag
     5161 gcgggaacga ttcatgcgat tggtgcgggt gcactaattg cagaaattca ggaaaattcc
     5221 aatctgactt atcgtctcta cgattatgac cgtgttgaca aaaatggtaa aaagagagaa
     5281 cttcatgtag acaaagcgct ggaagtagcg aacttgaaag ggttgtcaga accgaaacag
     5341 ccgcttcgtg ttctaaaata tcgtgctggt gtagctgcgg aattgctttg cagatgcaag
     5401 catttcgagg tctctcgtat gattgtcaac actgagagaa gacagacggt acactatcgt
     5461 gcggattcaa tcagcttccg tgtgttgctg tgtgtcaatg gctgtggtac cgttcgattt
     5521 gataatgaat cgttggacat ctataagggg gactgccttt ttgtgccggc cgattctact
     5581 gaaatcagca ttcacgggtc gattcagttt ctggatgtga gaggataata atatgaatta
     5641 taaagtagaa tatgagcgtt ggctgaatca tgccaatgca gatattatag aagaactgcg
     5701 ttcctattct gagaaggaca ttgaggactc tttctataaa aatttgtctt tcggtactgg
     5761 tggtctgcgt ggtaccatcg gtgcaggcac taatcgcatg aacgtacaca ccgtaggtaa
     5821 ggcaagccaa ggtctggcag actatctgaa taagaactat gaagccgctt ccgtggtggt
     5881 tggctatgat tcccgtatca aatctgatgt gtttgcaaag actgccgcag acatctttgc
     5941 tgcaaatggt attcaggtgc acttgtggcc agaactgctt cctgttccta cagtatcttt
     6001 tgcgactcgt tatctgaagg cagatgccgg tgttatgatt acggcatctc ataacccttc
     6061 taaatataac ggctacaagg tatacggtgc agacggctgt cagattacta ccgaggctgc
     6121 tactgcaatt ctggcagaaa tcgaaaagct ggacatcttt gctgatgtaa agatggatgg
     6181 cgatgcttct aagattgcat ccattcagtc ggaagttctg actgctttca tcgaagaagt
     6241 gaaggggcag tccgtcctgt ttggcgaaga agtggacaag aatgttgcta tcgtgtattc
     6301 tcctctgaat ggtaccggtc tggtgcctgt tacccgcacc ctggcagaga tgggatacag
     6361 caatgtgacc gttgtggaag aacagcgtct gccggacggt aatttcccaa cttgtcctta
     6421 tccgaaccct gaaattaagg aagctatgtc tctgggtatg gagtatgcca agaagtacaa
     6481 tgcggatctg ctgttggcca ctgaccctga ctgcgaccgt gttggtatcg cagttaagag
     6541 cgcacatggt gactatgttc tgttgtctgg taatgagact ggtctgctac ttctggacta
     6601 catctgttct cagagaatta agcatggaaa aatgcctgct gaccctgtga tggtcaagac
     6661 catcgtcacc atggatttgg gtgaacgtat cgcatccaac tatggtgttc gtaccatcga
     6721 tgtgctgacc ggctttaagt ttatcggtga gcagattggt atgttggaaa aggctggtaa
     6781 ggttgacagc tatatcttcg gctttgagga atcttacggc tatctgtccg gtagctatgt
     6841 ccgtgacaag gacggtgtta acgctgctta tctaatctgt gaaatgttct cttactacaa
     6901 gactcagggt atcagcctgc tggataagct gaacgagatt tatacaaagt atggctattg
     6961 tttgaacacc ctgcattcct tcgagtttga cggtgcggca ggttttacca agatgcagga
     7021 aattatggtt gaattccaca agggtctcga tataattggt ggtaagaagg ttattgaaac
     7081 tttagactac agtaagggcc tgaatggtct gccgaagtct gatgttttga agtttatgct
     7141 ggaagataac tgctctgttg tggttcgtcc ttctggtact gagccgaagc tgaagaccta
     7201 tatcagcgtc agcgcagaga acaaggaagc cgccgaagct atggaagcta ttatggctgc
     7261 tgaactggcg gaaatgttcg agtaagaaaa gaaaggagaa atcttgtaat ggaggaactg
     7321 ttttctaacg ctttgaaatc ggtgaagtca aatcgtgtgc tgtacacggc atcgttcttt
     7381 gcaagatcct ctcttttgta tttgcaggaa attggacagc tgactgctct gaaggcacat
     7441 acatctaggc gtacaggatt gtcatcttac ctttgtttcc tggtagaagt tggttcaggc
     7501 acattggagt ataaggggca gaaatatacg cttcaagtag gagatatggt gtttattgac
     7561 tgtttgaagc cgtatgtcca tgagtcatca gaagatgatt tgtggacttt gcggtggtgc
     7621 cattttaatg gcattggtat gacggaagtc tacaaaaaat accttgaacg aggtggtcaa
     7681 ccggtcttta aaacatcaaa aacagataag tatgctgact tgctgaggag ccttcatatg
     7741 gtggcagagg gacaggagta tgtccgggat atgaaaatca atgttctact cagtgaactg
     7801 ctgatgcatt tgatggaaga ttcttggaat cctgacaaag tgccgaattt ctctacaaaa
     7861 aaaatggaat tgcagaacat aaaacaatat ctggatgacc attacacgga gaagattacg
     7921 ctggatcagt tgagtgcaga gttttttatc aataaacagt acttagctaa gatttttcgt
     7981 gaaacttacg gtacaactgt tgtcggttat ctgaatcaga aacgaatcac acaggccaag
     8041 cacttgctac gctttacaaa tctaagtatg gaagagattg caacaaaaat tggcataaca
     8101 gatgcaaact atttttccag aacttttttc aaggtggaag gtgttcgtcc cagcgaattc
     8161 cgcaagatgt ggtaataaaa agccggcggt gttgagccgc cggtcagtac ctgtcgttaa
     8221 cccgcattca aagctttaat ctcagctttg atttcagcac gaacacgata gtaatcttca
     8281 gtttcgattt cggtatccag ccgagttgct gtcttttcgc agtcaggaca acgctctggc
     8341 tgttcgtcgg cagggaagct gtagtggcag gaatcacagg tgtaaatgtg atcgtcacgt
     8401 ataatcatca cctaccaatc cagtgcttag atttttcatg caattactat tatatcgaat
     8461 tggtgattcc acaaaaatcc gcttcttgat aaatgtacga ttcagagtga tatatagtac
     8521 tgtactgcca aagggaatac cacaacggca gaaatggatg attcacatgg aagaaattat
     8581 gcagatgatt ttagggaacg caaaattcca gctcggacag gtggtttcga cccctgctgt
     8641 attggaaact ttcaaccccc tgcagaccgc aaacggcatt cccgaagcct tgagcaacct
     8701 ttggtttaga caggaaatta gtattgcgga gatcgaggac gcagttctgg atgccaagaa
     8761 cggcaaggag ctagcaaaca gcctcaaccg catcaagctt ttccgcaagt tcaccctcga
     8821 ccgagagctg gacggcatgg tcagactgaa gggcacggac gtctggaaga acatcagcta
     8881 cctcaaggtc tacaccgcca cgccgcctaa gaaaccgacc cccccaaggt tccagtcgaa
     8941 cggctggagc cttttcttct ctcataa
//
LOCUS       DAZKCN010000104         8666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000104 DAZKCN010000000
VERSION     DAZKCN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.107"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..2250
                     /locus_tag="ACG8K3_002297"
     CDS             208..2250
                     /locus_tag="ACG8K3_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9146122.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACG8K3_002298"
     CDS             2261..2881
                     /locus_tag="ACG8K3_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9146123.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACG8K3_002299"
     CDS             2893..3351
                     /locus_tag="ACG8K3_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9146124.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACG8K3_002300"
     CDS             3370..3648
                     /locus_tag="ACG8K3_002300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9146125.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACG8K3_002301"
     CDS             3673..5040
                     /locus_tag="ACG8K3_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9146126.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACG8K3_002302"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACG8K3_002302"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP9146127.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACG8K3_002303"
     CDS             6227..6718
                     /locus_tag="ACG8K3_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146128.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="ACG8K3_002304"
     CDS             7161..8360
                     /locus_tag="ACG8K3_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9146129.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2692 a         1420 c         1845 g         2709 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacat
//
LOCUS       DAZKCN010000105         8622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000105 DAZKCN010000000
VERSION     DAZKCN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.108"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..920)
                     /locus_tag="ACG8K3_002305"
     CDS             complement(162..920)
                     /locus_tag="ACG8K3_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9146130.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            1088..2023
                     /gene="pfkB"
                     /locus_tag="ACG8K3_002306"
     CDS             1088..2023
                     /gene="pfkB"
                     /locus_tag="ACG8K3_002306"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9146131.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            2020..3465
                     /locus_tag="ACG8K3_002307"
     CDS             2020..3465
                     /locus_tag="ACG8K3_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9146132.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            3493..3948
                     /locus_tag="ACG8K3_002308"
     CDS             3493..3948
                     /locus_tag="ACG8K3_002308"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9146133.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            3967..4941
                     /gene="lacD"
                     /locus_tag="ACG8K3_002309"
     CDS             3967..4941
                     /gene="lacD"
                     /locus_tag="ACG8K3_002309"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9146134.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(5052..5613)
                     /locus_tag="ACG8K3_002310"
                     /pseudo
     CDS             complement(5052..5613)
                     /locus_tag="ACG8K3_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            5757..6371
                     /locus_tag="ACG8K3_002311"
     CDS             5757..6371
                     /locus_tag="ACG8K3_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146135.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            6612..6995
                     /locus_tag="ACG8K3_002312"
     CDS             6612..6995
                     /locus_tag="ACG8K3_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9146136.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            7358..7867
                     /locus_tag="ACG8K3_002313"
     CDS             7358..7867
                     /locus_tag="ACG8K3_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9146137.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            7967..8617
                     /locus_tag="ACG8K3_002314"
     CDS             7967..8617
                     /locus_tag="ACG8K3_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146138.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
BASE COUNT         2850 a         1346 c         1805 g         2621 t
ORIGIN      
        1 gtcctggtag tctcaatgca ctattctatc ttgaaactaa gaagttatag aaggagcaat
       61 gatagaaagc acgagaatac aaatagcgat taaatatttt tctagacaac cttagttaat
      121 tcacacagat atattttagg tacagtctta cagatataga gttagtgctg tgtgaatcct
      181 gatgttcgca gtgcatagga gtcaagtaag attctagcca gtatagaaag tggaagtctg
      241 gagcgtacct cataatctgg gaagaaggag ccttctgatt taaagccaac aaaaggaatc
      301 tcgatatgtt ccatcaaact tgaatgacga ttagcagtaa gcaaaattgt cccgatctca
      361 gctttataac agttttcagc tgcggtagtc aattcctttg tttcaccgtt tagtgaaaca
      421 aagatcacga tgtcatcttt ggataatttt ctactaatgt tttttatgat attgggatca
      481 gtatgcatct cacaatattt accggtaagt tgaaatttga ccatcatttc ctgagcaatc
      541 aattctgaaa aacctcgggc aaaaatcatg attcttctag cagccttgat tcgttgaata
      601 gcatcttcaa tattcccgct ttctaccata ttgatggtgc ggattacctc ttgctcattt
      661 ttaagaatag cagtctttat tccttcatca actttatcaa gagcggcaaa attgatattt
      721 gtattttctt tttcttttaa cgaatgttta aaagacgtaa acccctcata tccttttttc
      781 ttcatagtac gaacgacggt tgctgttgaa acgttggcat attcacttag ttttacaatg
      841 gaataggagg ggatttcgag tatgtgactt tgaataaaat cccagagata tttctccgat
      901 tcacttaatt ttgtatccat attttgcctc cgattagtta ggataattct attgtaaagc
      961 gggagtttcc tttacttcaa gtttatttaa tatattttgt aaaagttttc aatgaattta
     1021 gtcatttatg aaaacattta cattcgatct tgatacataa tgaatatgta agaaaggagg
     1081 agcgtaaatg tcagtttata catgtacttt aaacttggca atcgaccttt ttatcgaaac
     1141 agatgaaatg aaaccatccc aagtcaatcg aacaaacgat gatgatatac aagctaatgg
     1201 aaaaggtgtg aatgtttcat tgatcatgaa gcaattaggt atcgatagta cagctttagg
     1261 atttaaagca ggttttaccg ggaattatat tgaggattat ttgcatcaaa aagatattag
     1321 cactgatttt attagcgtac cgggaatgac tagaatcaac gtttttacac aagttaataa
     1381 aaccaatgaa gaattcaaat tggttaataa aggacctgag attcctcagg aacaaatcaa
     1441 aacttttcta aatcaaatac aaaagctgca agctgacgat tatttgtgtg tttctggcag
     1501 tttgccgcga ggggtctcac ccgaaattct tattgatatc agtcgtattt gttttgaaaa
     1561 taaggtccat ctgattattg attcaagcta tccggaaata attgattgtc tgccgtatca
     1621 gccgttctta ttaaaaccaa acgaggagga attgcaatct tggtataatg tcgagcttag
     1681 tgatcgtgaa ggatatattc attatgctga acgcttgatt gccgatggag cgaaaaatgt
     1741 tctcctttct ctaggaggag agggaggaat atttttaaat aaggatatcg tttattctgg
     1801 gaattctcct aaaggtaaag tagtcaatac agcgtgtgct ggtgacactt tgttgggaac
     1861 atttcttgca ggatatttaa aaggtgaaca ggtagatatt aatttgaaga aaagtttagc
     1921 agctggaagt tcaaccgctt ttcgtaaagg attaacagat ttttcggatg ttgcagagtt
     1981 ggaaaaacaa atcgaaatca aagagggagc gagtaaaaaa tgacaactta caaaattgtt
     2041 gccgcaacag gatgtccaac aggaattgcc cacacttata tggctcaaga agcattggag
     2101 caggcagcta agcgcaaagg ggtatctatc aaagttgaaa cccatggtca agtagggata
     2161 gagaatgaat taacaccaga agaaataaaa aacgctgacg ctgtaatcat tgcagctgac
     2221 aaagatgttc aagcagaacg tttcgcaggt aagcgtgtaa tcgatgtgcc tgtcagcaag
     2281 gggattaaag atgctgacca attgatcgaa gatgcgttgg ctggtaaagg ttcaatcact
     2341 gcagaaaaca aaacagttga cgctgctggt gagaaagcgg agatgggggt tgcagcaggc
     2401 attggtcaca gtatttataa aaatctcatg aacggtgttt ctcatatgct gccgtttgta
     2461 gtggcaggtg gggtaattat tgcaatttcg tttgccatct ggggtgtgta ctcttttgat
     2521 cccaatgatc ctacttacaa tccgactgcc gctatgttaa agggtgttgg tgatgcggca
     2581 atgggaatga tggttcctat tttagcagct tatatcgctg aaggaatcgc taaacgtcct
     2641 ggtttggttg ttgggtttgt tggcggactg ttagccaatt ctggcggagc cggtttctta
     2701 ggcggaattt ttgcaggttt cttagcaggc tactttatct tactattgca aaaggtcatg
     2761 aaaggcttgc ctaaacaatt ggatggtctg aaggcgatct tcttatatcc ggtgatcggt
     2821 gtcttgtttg ttggtgtagt catgtcttta ttagcaaatc caatggaagc agtcaaccaa
     2881 ggaatgatga atttcttggc aggattccaa aattctaatc cattaatttt agggattatc
     2941 gttggctgta tgtcagcatt tgatatggga ggtccagtca ataaagcggc ttatgttaca
     3001 gggactgcgt tgttggcaca aggtaaccat acatttatgg caggggtttc ggcagcttgt
     3061 atcgcgcccc cattgattac tggatttgcc gcactgtttt ttggaaaata tttcgacacg
     3121 aatgatcgca acgctggttt agtcaacttt atcttgggct caacgcacat taccgaagga
     3181 gcaattccat ttgcggctaa agatccgctg cgtgttctgc caactatgat gctaggttca
     3241 tcaattgctg ctgtactgac atatatgttt ggtgtccaag ttcccgcacc tcatggaggt
     3301 tttctagtat tacctgttgt tactggtaag gttgcatggg tagggtcgat tttacttggt
     3361 tcaatcgttg gcggagtaat tttagggttg atccaaaaac gtagtgtaga aaaacgtgaa
     3421 gcagaagcag ctgctgttga aatgaaagaa gcaaccaaca actaaaggtt attctagaaa
     3481 gaaggatttc aaatgacttt gatcaaaaca aataaagttt ttatgaataa aaattttacg
     3541 acgcaagatg aggtaatgaa cttccttgca gatcagtcaa ttgtcttgag cattagtgaa
     3601 gaccgagatg cagtttacga aaaattgatg gaaagagaag aagaaagcac gactggtatg
     3661 atggatggtt ttgccatccc tcatgccaaa gatgctagta tcaaggatgc cgatattgtg
     3721 attgttcggt taaacggtaa ggtcgattgg aaaagtttag atggagaagg aacggatttt
     3781 gtaatcgctc tgtttattcc agattcagag gctggaacaa cccatctaaa atttttatct
     3841 caggtagcca gacttttgat gcacaaagat gtgacagaag gtctgaaaca agcccagtct
     3901 gctgaagaaa tagctgaact tttaaaccaa aaattagaag aaaactaaaa aggaagtagt
     3961 gaaatcatgc ctttaacaga aaaaaagaaa acttatattc gtcaactaag taatgaaagt
     4021 ggcatcatca gtgcgttagc aatcgatcaa cgtggtgcct tgaaaaaaat gatcggtaaa
     4081 tttcaaaata aatcagcaac cgatgaacaa atcatcaatt tcaaaaaaat catttcttct
     4141 gaattgactc catatgcttc agcaatctta ttagatccag aatatgggtt gccggcagcc
     4201 gaagttcgcg ataaaaatgc tggtctgttg ttggcctatg aaaaaactgg ttacgatgct
     4261 tcaactccag gacgtttacc ggatatttta tctatttggt ctgtaaaacg tctaaaggag
     4321 caaggggccg atgcttgtaa gttcttgtta tactacgatg tagatgaatc tgatgacatc
     4381 aatgaccaaa agaaggcgtt tatggagcgg attggttctg aatgtgttgc tgaagatttg
     4441 ccatttttct tagaattggt ttcttatgat gccaacgatc ctgatacttc ttcgaaggaa
     4501 tatgccttga aaaaaccgca taaagtaaat gaaatgatga aggaattttc taagcctcgc
     4561 tatggagttg acgttttgaa agtggaagtt cctgttaata tgaactatgt ggatggttac
     4621 actaaaaatg aagttgctta taccaaagaa gaagctgctg cttatttcaa agaacaatcc
     4681 gagactacca acctgccatt tattttcttg agcgcaggag taactgctga attgttccaa
     4741 gaaaccttga aatttgcaaa agaggctggc tctacattca atggggtact ctgtgggcgc
     4801 gcaacttggg cgaatggtgt agaaccattc gttactaaag gtgaatatgc tgccaaagaa
     4861 tggatgcaga cacaaggacg caagaacatc gaagagctga ataatgtttt gaaagaaaca
     4921 gcaacagcta ttaaattgta agaaagcttt aactataacc ttaagtttgt aacctaatga
     4981 tttttaataa tcaatttgag cttttaaata aagaattgga agcgtcccga cgcttccttt
     5041 ttcgtttgtt tttaaggcat gataggttct atgttgtcgt tcaataagcg caagaaggtc
     5101 agcatttttt tcttagagta tgcaaaattg cttttctacc caatgattta aaacggagag
     5161 gcacccttgg atatggtaga tggtgaagta acgcatttct tctgtgatca cggttttttt
     5221 gtcttcttgt agccagattt ttaaataatt ttcttcgatc agattattta atctcttttg
     5281 aaattggata tctccgtggt tgatgacgaa taacttgtat acgaacgtat tcgtatagac
     5341 atagtcgatc aattgagcgt atagatcctc gtagctatgt gtcgtatcgt aggaaatgat
     5401 ttcatccaag tcttggaaaa agctattttc cagtttttca taaagatcat agatatccgt
     5461 ataatgtgta taaaatgtcg aacggtgaag atcagcagtt tcggtcagtt cacggatggt
     5521 gatgttttga atttttttat ctactaataa ttttgataat gcttctgcta acgctttttc
     5581 tgtttttttg acgcggcgat cctctttttt cattttgcag cctcctaatc aacataatca
     5641 aggaattgtg tcggttatcc gacaatttca ccgctatctg aacatagata accgacacct
     5701 tgtctattat aatataagta tagcatatca atatttaaag aataggagaa taagtgatgg
     5761 aattagcctt taagcaactg gaaaagaaag actacaaaaa agccattcaa ttcgcgatta
     5821 aaggtatgca ctttgattgg tataccaaga acaaactcct tttgaatctt tatggaagat
     5881 acttttggta tctagaattg aatcgcgcga cacaaatcat tgctttatat gcggatgatg
     5941 agttggcagg tgttttatta gcagacatca aaggaaaatc aaaaaagcat cattcattta
     6001 gtcaaaagtt atatgttaaa ctatttgatt tcttacaaaa tgcatttgtg aaagacagca
     6061 aaggcgggta tgacgacacc aatgacgaat tgctttcaga ctatttaaaa aaacattccc
     6121 cagatggaga aattgtcttt ttagctgcca accctgattt aaagatcaag gggatcggta
     6181 gtaaattatt aaaagaattt gaacgccgag agcaaggaaa agaagtattt ctgttcacgg
     6241 atagcggctg tacgtatcaa ttttatgaac gacgcggttt cgaacggaaa aaagaaaaag
     6301 ataacgtcat gaagatcctt aataaagaag tagatctccg ctgtatgtta tatagtaaga
     6361 tcattcagta atgtttttta ctatcagaaa gcagaagtac cacgaaggtc aatggaaaaa
     6421 gcgagtaatt gatccattta gatgaagata ggtgtagtct caaagatatt agctggcaaa
     6481 gatatcccca gttaaaaata gatttctgta tccaacaata aaaatatttt tgtatttatt
     6541 agtatatata ctttacaaaa atttgtatat atactaattt ttttgtagga tcaatgaaat
     6601 gaggggaaaa tatgccaaaa tataaagaaa tagccttggc tctaaaagag aggatcgttg
     6661 agggaaaata tcaggaaggt tctaaattac ctgagcaaga aagtctggct agagagttta
     6721 aaacaagccg tgttactatc cgcaaagcta tacaactcct tattgatgaa ggtttgttat
     6781 atacgagacg tggatctggt acttttatac gaagcaatat cagacaggac aatgagaatg
     6841 tcactcaagt aaataatgta tttggcactt cctcacaaga aaatacaaat gtgacaagta
     6901 aaataatccg ttttgatgtc cggtttccga cagattatga aaggcggctt tctgcaacct
     6961 acttccgatc cccttcctgc aaatcatggg tataaaaaat aggcatatta agcgggctag
     7021 gaggagaaat attcacatat cattttttca ttccgcaagc tgcgtcaagt tatttggaat
     7081 ttgtagaaca gatgagagag gtagaagcag cactacaaac tacttttcaa taagtaggaa
     7141 ttatagaaat tttactcaaa aaatcttcat taaaaaatct gactaaataa gttgagaaaa
     7201 cagaatatac ttcaatatcc agtgatttat aaatatcatt cactcatgat acccagctcc
     7261 gtatcataag tgatgcggtt tttatcattg atcctaaaaa tcttagcaag acaaaaagta
     7321 cgtaagaaag taaagactaa atacttcgga ggaacaaatg aataacaatg ataggttatt
     7381 gcgattgcgt tatgcgattg atttgaaaga tacggatttg attaaagctt ttgagctagg
     7441 cggtgtacca ctgactaaag aagaagctca ggcggtactg accaaagtac aggataagaa
     7501 caaagataat gcagaaaata atgtgtatga aaaaaccatc aacaaccaag tttttgacgc
     7561 attcctaaat ggcctgattc tcttggcgcg tggtccgcaa aaagataagc cgatagtaga
     7621 cacgccgcaa aaaagcaagg aaatcaaata tatcaacaat gtgttgctaa aaaaattaaa
     7681 aatcgccttg tctatgacca ctgatgatat tttagaagtt tttgctgagg cagaactcta
     7741 tccatcaaaa ggcgaaatcg gggcattttt gagaaaagaa ggccaacgaa attttaagcc
     7801 ttgcggagat aaatatatgc gtaattttct caaggggtta ggaatttata atcggagaaa
     7861 agtataaaga aatttcatga gaaatcaaaa caatggttgt tttgatgaga gctagttttt
     7921 gtatgagatt aaaatattat cttaatcgag gagctggatt ggttttatga atgatgaata
     7981 taagaatgat gaagataaga tgctttttga agagatagag aatcgatgta ggctcaattt
     8041 tgagcttcgg ggaaagatgt ctttaataca acaaaaaaag tatttagcga ataaatcaga
     8101 gtttactctg ggacatgttg aaaaattaat ctcggattgg attagtagta gaagcgaatt
     8161 tacaaaaata aaacagccga ttaaatttga catgaaaaaa cttttgttaa ataagagtga
     8221 gataggtaat agagatcaat atattagagc taaagggcaa gaaataattg attcattagg
     8281 tgaagtgagg tcatacaatt atttgtatgt aacacataga gctgatggaa tggtcattac
     8341 tgtggggaaa tcttcttcta atgatatttt tttagatgga gatctttttt atcaactcaa
     8401 tacaaatcac ttgtctggca ctgaaaatat aattttaagg acggaatatg gcaatgaaat
     8461 atttgcaaaa tatgatgaga ttttaaaaaa ttatttggac tgggcttgga taattccggt
     8521 tgagtcagga gatgctaaga aactggagag attattgggc gatgaactaa ttaataaaaa
     8581 agtaccaatt ttaaattatt attcccatag acaataaaag gc
//
LOCUS       DAZKCN010000106         8514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000106 DAZKCN010000000
VERSION     DAZKCN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.109"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..2749
                     /gene="clpB"
                     /locus_tag="ACG8K3_002315"
     CDS             140..2749
                     /gene="clpB"
                     /locus_tag="ACG8K3_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9146139.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2900..3586)
                     /locus_tag="ACG8K3_002316"
     CDS             complement(2900..3586)
                     /locus_tag="ACG8K3_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146140.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3564..4058)
                     /locus_tag="ACG8K3_002317"
     CDS             complement(3564..4058)
                     /locus_tag="ACG8K3_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP9146141.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4078..5298)
                     /locus_tag="ACG8K3_002318"
     CDS             complement(4078..5298)
                     /locus_tag="ACG8K3_002318"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146142.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5445..7280
                     /gene="lepA"
                     /locus_tag="ACG8K3_002319"
     CDS             5445..7280
                     /gene="lepA"
                     /locus_tag="ACG8K3_002319"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9146143.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7374..8501)
                     /locus_tag="ACG8K3_002320"
     CDS             complement(7374..8501)
                     /locus_tag="ACG8K3_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9146144.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2744 a         1625 c         1708 g         2437 t
ORIGIN      
        1 ttaagattta tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa
       61 ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta
      121 attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat
      181 tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt
      241 atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt
      301 ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc
      361 tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga
      421 agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt
      481 agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc
      541 tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca
      601 aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt
      661 caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg
      721 tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa
      781 aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt
      841 aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg
      901 tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg
      961 tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag
     1021 tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg
     1081 tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg
     1141 ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg
     1201 attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc
     1261 agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga
     1321 tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt
     1381 agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga
     1441 atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg
     1501 tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt
     1561 ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa
     1621 ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga
     1681 attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt
     1741 taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact
     1801 tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat
     1861 tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt
     1921 gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa
     1981 aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg
     2041 gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc
     2101 aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta
     2161 tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt
     2221 acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa
     2281 tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc
     2341 tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt
     2401 caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga
     2461 caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca
     2521 agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga
     2581 accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact
     2641 agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact
     2701 tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga
     2761 gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa
     2821 acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct
     2881 tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt
     2941 aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg
     3001 tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc
     3061 catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc
     3121 agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc
     3181 aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa
     3241 gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac
     3301 tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca
     3361 aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa
     3421 tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt
     3481 tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag
     3541 ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt
     3601 acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct
     3661 attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt
     3721 tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg
     3781 cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct
     3841 agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt
     3901 cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc
     3961 gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata taacaaatca
     4021 cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca
     4081 aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt
     4141 ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat
     4201 catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa
     4261 gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc
     4321 gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg
     4381 aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat
     4441 ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca
     4501 gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc
     4561 aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc
     4621 tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa
     4681 acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc
     4741 aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc
     4801 acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga
     4861 catgatttcc catcctttac gccgatagta ggggatactg taaggataca aataagagat
     4921 ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc
     4981 atggttagca tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc
     5041 accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg
     5101 gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg
     5161 tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag
     5221 caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct
     5281 aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa
     5341 ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg
     5401 ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa
     5461 tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga
     5521 aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc
     5581 aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa
     5641 atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg
     5701 acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg
     5761 gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt
     5821 atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg
     5881 cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg
     5941 aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg
     6001 tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg
     6061 actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg
     6121 tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg
     6181 tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt
     6241 atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag
     6301 cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt
     6361 gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac
     6421 tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg
     6481 ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac
     6541 gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa
     6601 cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc
     6661 aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg
     6721 cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg
     6781 attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg
     6841 acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc
     6901 aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa
     6961 gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga
     7021 aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa
     7081 tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg
     7141 gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga
     7201 aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggacg
     7261 aggacgaacc gaagaaatag caagggtttg tttgaaaaca gtgtaaaaaa taaaccatgt
     7321 ttacatggat ttgccaccgg tttgccaccg gtggtttttt tcggtggcaa aaatcaagaa
     7381 ttctttctaa gattttccat gatatcgacc gtttcatttc tcatcttatc tgttacatgc
     7441 gagtaagtat ccatcgtaat tgaaattctg ctgtggccca atcgttcaga gatttctttc
     7501 atttttgcgc cattttcgag aagaagtgta gcatgagtat gtctgagaga atggaagtta
     7561 aaagagaggg agagtgcatt cgatatcctc ctagtattcc atttcactac acttggcgta
     7621 actagctcac cgtcttcctt cgtacatact gcatttgaat caatgtagag ctttccatac
     7681 ttcattcgat tttctaattg ttgtttctta tgttttttca gaattgctag caaggtttgt
     7741 ccaataaaaa tcgttcgatt ggaactgctt gtctttggcg taccatatac ccatgcgccg
     7801 tcattcttta ccatttgttt ctctacagta attgttccat ttgaaaaatc gacatcatcc
     7861 cacgtcagac cacaaacttc gccaacgcgc attcccgtat aaaatccaat attcaaagga
     7921 atatagaaag gatgtccttc aggagtaatt tctagcatat gatcaaagtc ctcaagagaa
     7981 atgattttta gatctttttt agtcgttggt cgtgcttcat attttggtat ttttacatac
     8041 agcataggat tttgcttgat taatccccaa ggataaaccg ccatattcag cgcattctta
     8101 aggacagagt gagtaatagt cattgttttc ttcgagtaac cctttttaaa ttcagcattg
     8161 atgaaatttt gtaaaagagc aggggagaga tccgtaagtt ttttctttcc taaataaccg
     8221 tttatatgat ttttgatggt aaatcggtag ttttcatagg tattgtattt tagatttagt
     8281 ttaacgtatt cctccatcca aaaatcaagg tattgtttta ctcgagtatc cgtacctaaa
     8341 aagtattgtc ctgtttcgtc aatatctgat aaaacttttc gtaaagcagc ttcggcctct
     8401 ggtcgggtgt ctccgccaac tttctccact ttttttcttg agccatcatc attgatatct
     8461 tcgccgtttg tataattaag cgagacaaat aaaaaagcca tttatgttac actc
//
LOCUS       DAZKCN010000107         8252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000107 DAZKCN010000000
VERSION     DAZKCN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.110"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8K3_002321"
     CDS             476..1339
                     /locus_tag="ACG8K3_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP9146145.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8K3_002322"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8K3_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP9146146.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8K3_002323"
     CDS             3339..3860
                     /locus_tag="ACG8K3_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP9146147.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8K3_002324"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8K3_002324"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP9146148.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8K3_002325"
     CDS             5941..6759
                     /locus_tag="ACG8K3_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP9146149.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8K3_002326"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8K3_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146150.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8K3_002327"
     CDS             7228..7806
                     /locus_tag="ACG8K3_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP9146151.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8K3_002328"
     CDS             7901..8038
                     /locus_tag="ACG8K3_002328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146152.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2477 a         1483 c         1629 g         2663 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag ga
//
LOCUS       DAZKCN010000108         8068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000108 DAZKCN010000000
VERSION     DAZKCN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.111"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..884
                     /locus_tag="ACG8K3_002329"
     CDS             <1..884
                     /locus_tag="ACG8K3_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP9146153.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACG8K3_002330"
     CDS             932..3061
                     /locus_tag="ACG8K3_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP9146154.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACG8K3_002331"
     CDS             3036..3725
                     /locus_tag="ACG8K3_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146155.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACG8K3_002332"
     CDS             complement(4133..4819)
                     /locus_tag="ACG8K3_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9146156.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACG8K3_002333"
     CDS             complement(4955..5149)
                     /locus_tag="ACG8K3_002333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146157.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACG8K3_002334"
     CDS             complement(5199..5639)
                     /locus_tag="ACG8K3_002334"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP9146158.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACG8K3_002335"
     CDS             complement(5643..6488)
                     /locus_tag="ACG8K3_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP9146159.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACG8K3_002336"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACG8K3_002336"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP9146160.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAZKCN010000109         7901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000109 DAZKCN010000000
VERSION     DAZKCN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.112"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            709..1602
                     /locus_tag="ACG8K3_002337"
     CDS             709..1602
                     /locus_tag="ACG8K3_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9146161.1"
                     /translation="MGKISLGIDIGGTFIKYALIDKQHRIKEKWKVETVKYNTADEFY
                     DYICSNIRNVDEIDKIGVSAPGLIDEDSNVKSYAAPNVAIMYGTNINNEINKRTNKKV
                     SAINDAKAAGLCEFKIGNAKGYKSSAFLIIGTGTGGCICDENGVVYGKDSFAGEFHNM
                     PFVNAEIGGLDKMGDYASITGLVNLYNKKANREEQVQYGNEVCKKYLNGNESAVSSVD
                     EWIGNIVAQLIVITVFYNPEVICIGGGISEENWFINKVRETYKKTCREYLEADFITTE
                     INRCKHNNDANILGAVIKASI"
     gene            1676..3115
                     /locus_tag="ACG8K3_002338"
     CDS             1676..3115
                     /locus_tag="ACG8K3_002338"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP9146162.1"
                     /translation="MIYEELDKFQDDFLWGSASAAYQVEGAWDKDGKGESVWDVYTRQ
                     PGTTFKNTNGNIAVDHYNRYKEDVALMAEMGLKAYRFSIAWTRIYPNGRNEVNEEGLK
                     FYENLIDELIANNIQPIITLYHWDLPQHLQDLYGGWESREIIHDFNEYCVTLFKRFGN
                     KVKHWVSLNEQNIFVTLGYVTAMHPPAVKDQKRMLEANHIANLANATVIESFKKYVPD
                     GMIGPSFAFGPVYPFSCDPKDVLSAENAEDLNCNWWLDVYCKGKYPVFAFKYYERLGI
                     APTIEDGDLELLERVKPDFIGINYYQTSTVAFNPLDGVGQSEGINNTGKKGTTKESGL
                     PGVYKNAKNAHLETTNWDWAIDPTGLRIGLRRLTSKYNLPILITENGLGEFDKLEEDD
                     TVNDAHRIDYLSAHIKACKEAITDGVDLLGYCTWSFTDLLSWLNGYQKRYGFVYVDRD
                     ENDQKDLRRIKKNSFYWYKNVIRTNGEEL"
     gene            3163..3465
                     /locus_tag="ACG8K3_002339"
     CDS             3163..3465
                     /locus_tag="ACG8K3_002339"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9146163.1"
                     /translation="MKKILLVCSAGMSTSLLVTKMRNSAKEKGIEVEIDALPVAEAST
                     AVDNVDIVLLGPQVRFQKGTVEKLVKGRIPVEVMDMRLYGMMNGKAILEDALQKIN"
     gene            3484..4848
                     /locus_tag="ACG8K3_002340"
     CDS             3484..4848
                     /locus_tag="ACG8K3_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9146164.1"
                     /translation="MSFMDKFQSAIERLLVPLSTKLNAQRHICAVRDAFILSFPLTMA
                     GSLMVLLNNVFLSADGFMFKILQFDKLFPGIVKFQAVFAPVVKGSADIFSILIVFLIA
                     RNLAKLMEGDDLLTGLTAISVYFIIYPDYFDNEGVNYLTNQYMGAQGLFVAILVGLLV
                     GELMSRLAKSDKLEIKMPEQVPPAVARSFKVLIPIIITTVFFSVLNYFVKMAAPGGLH
                     ELIYNIIQTPLTRMSQSLFSVIILALLSQLLWAMGIHGPNTIAAIRDTMFSEAGNANL
                     LHYAESGTTWGAPFPITYSGLATAFAEYGGSGATLGLIIAILIFSKNKESKSIGKLSF
                     VPGLFNINEMVIFGLPIILNPIYIIPFIIAPLVNIMIGYTAVMILKIMPPVTIGIPWT
                     TPGPLMPFLGTGGNIVALFVGFICLAVSVLIYSPFVIAANKAAIIEDEMENEIQSQEV
                     VESI"
     gene            4904..5218
                     /locus_tag="ACG8K3_002341"
     CDS             4904..5218
                     /locus_tag="ACG8K3_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9146165.1"
                     /translation="MEGIELTAFSIISNVGMAKSLAIEALRDARKGKYNEAEKKMAEA
                     SEYFVKGHHAHTSLIQKEAAGEKVELSLIIMHAEDQLMSTETTKLLIEEMIEMFKQLN
                     NK"
     gene            5552..6598
                     /locus_tag="ACG8K3_002342"
     CDS             5552..6598
                     /locus_tag="ACG8K3_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146166.1"
                     /translation="MMNSTEIAKIAGVSRSTVSRVINNYSNVSEETREKVLRVIKEHE
                     YVPHASARMLAGSQNKVIGLFIIDLIHEEVGLKNRITKSPYYLEFTSSVIETASEMDY
                     LVLVHIIHSVAGYEKIKESFYNKTISGGIFIGQNDDDESIKTIIDRGYKVVLLDQSIR
                     PEDATYNQCMIVNADNYNGAYCATKYLIDKKHKQIAHITGGTVKFSSKDRIRGYKKAL
                     EDAGIEVNKNLIVNSEFVENAGYEAAKKLFNGNSEFSAIFASNDKIAFGAIKAIQERG
                     LKVPDDISVIGFDDIESSKYFNPPLTSMKMKLGEMADIATKSIVASIEGETKPSINVV
                     PVSLVERESCGILQ"
     gene            7153..7707
                     /locus_tag="ACG8K3_002343"
     CDS             7153..7707
                     /locus_tag="ACG8K3_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146167.1"
                     /translation="MKQLLSKLKKIWAVLPEHSLKQRIGNNLALIYSGALLIQDILGY
                     PLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTI
                     WGKVSIKKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRI
                     HMNRQVLPTYEILLPSSLKSYFRL"
     gene            7741..7901
                     /locus_tag="ACG8K3_002344"
                     /pseudo
     CDS             7741..7901
                     /locus_tag="ACG8K3_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2756 a         1218 c         1643 g         2284 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatact tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acgatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg tcattagaaa
      181 atgatggtaa attatttgat ggtaacggtg atgtaacaac ggtaatgaaa ttattgcttt
      241 aacagtttag cgtgataaaa atctatacct tcttaagttg atttattaac ttgagaaggt
      301 atttttttct gaaaaatgat aatgaaaatc aaaatgttca tttctatcaa ttttgaagtc
      361 aaaaaaataa agtacagccc agaaatttgc tctgtacttt aaaaaactag ttaaatgaag
      421 atgaaatcgc tcccttattt tttagtattg gtggaattaa aaggtacttt ttaaacaaaa
      481 actacacata aaaaataaaa aaaataaaaa aagtattgaa atgtaattgc tttcagtata
      541 tgctatatct ataaacgcgc gttcatggga tgttcatcgt ataaaaaaag gtggaaatga
      601 gcgtatcgtt tttcttttta acaaatcatg aacgcgcgtt tatgagtttg aatcaagctg
      661 caatataaga aggcaaactg caagaccttc attttaagga gaagtgttat ggggaaaata
      721 agtttaggaa tagacatagg agggacgttc attaagtatg cattaataga caagcaacat
      781 cgcatcaaag agaagtggaa agtcgaaacg gttaagtaca atacagccga cgaattttac
      841 gattatatat gttcaaatat aagaaatgta gatgaaattg acaaaatcgg tgtaagcgca
      901 cccggcctta ttgatgaaga ttcgaatgtt aaatcctatg ctgctcctaa cgtagctata
      961 atgtacggca caaatatcaa taatgagatc aacaaaagga caaataaaaa agtgtccgca
     1021 atcaatgatg caaaagcggc aggtttatgt gagtttaaaa ttggaaatgc aaagggatac
     1081 aaatcttcag cgtttttgat tataggtaca ggaacaggcg gatgcatatg cgacgaaaac
     1141 ggagttgtgt atggaaaaga ttcgtttgcg ggggaattcc ataatatgcc ttttgtaaac
     1201 gctgagatag gcggccttga taagatgggt gattatgctt cgataaccgg attggttaat
     1261 ttatacaata aaaaagctaa tcgggaggag caggttcagt atggaaatga ggtttgcaag
     1321 aagtacctaa acggaaatga atcagctgtg tcttcagtag atgaatggat aggaaacata
     1381 gtggctcaat taatagtaat caccgttttt tataatcctg aagtgatatg catcggtgga
     1441 ggaatatccg aagaaaactg gttcatcaat aaagtaagag aaacgtataa gaaaacctgt
     1501 agggagtatt tagaagcgga ttttattact accgaaatca atagatgcaa gcataataac
     1561 gatgccaaca tactgggtgc tgttataaaa gcttctatct aaaatacaca acagttttgt
     1621 ttcaagaaag aatgtttgaa aataaattta tattgaaaac gaaggagaag attcagtgat
     1681 atacgaagaa ttagacaaat tccaagatga tttcctatgg ggatcggcat ctgcagcgta
     1741 tcaggttgaa ggagcatggg acaaagatgg aaagggggaa agtgtatggg atgtgtacac
     1801 ccgccaaccg gggacaacat ttaagaatac aaacgggaat atcgcggtcg accattacaa
     1861 tagatacaaa gaagacgtag ctttaatggc tgaaatggga ttgaaagctt atagattctc
     1921 aattgcatgg acacgtattt atcccaatgg aagaaatgaa gtaaatgaag aaggcttaaa
     1981 attttatgaa aacttgatag atgaacttat cgcgaacaat atccaaccaa tcattacgtt
     2041 gtaccattgg gatttacctc aacatcttca agatttgtac ggcggatggg aatcaagaga
     2101 aatcattcat gattttaatg aatactgtgt aacgttattc aagagatttg ggaataaagt
     2161 taagcactgg gtatcattga atgaacaaaa tatatttgta acactaggtt acgtaaccgc
     2221 tatgcacccg ccagctgtta aagatcaaaa gagaatgctt gaggcgaatc atatagcgaa
     2281 cttagctaat gctaccgtaa ttgaatcctt caaaaaatat gttccagacg gtatgattgg
     2341 accaagcttt gcatttggac cagtttatcc attcagttgc gatccaaaag atgttttatc
     2401 cgcagaaaat gcagaggatt taaattgcaa ttggtggctc gatgtttatt gcaaaggtaa
     2461 atatccagtg ttcgctttta aatattacga aagacttggg atcgcaccca ctattgaaga
     2521 tggagatctt gaattgttgg aaagagtaaa accagatttt atcggaataa attattacca
     2581 aacaagcaca gttgcattca atccgcttga tggagtaggg caatcggaag gtatcaataa
     2641 cacaggtaaa aaaggcacca ctaaagaaag cggattaccc ggagtttata aaaatgcaaa
     2701 aaatgcacat ctggaaacaa caaattggga ctgggcaatt gatcctactg gtttaagaat
     2761 tggactaaga agattgacca gcaaatataa tcttccaata cttattacag aaaacgggct
     2821 tggagaattc gacaagcttg aagaagacga cactgtcaat gatgctcaca gaatcgatta
     2881 cttgagcgca catatcaagg cttgtaaaga agcaataaca gatggtgttg atcttctagg
     2941 ctactgcacg tggtcattta cagatttgtt aagctggttg aatggttatc aaaaacgcta
     3001 tggatttgtg tatgtggaca gagatgaaaa tgatcaaaaa gatttgagaa gaatcaaaaa
     3061 gaacagcttc tattggtaca aaaatgttat tcgcaccaat ggagaagagt tataagaatc
     3121 gttcacaatc aatgaatatg aaaaaacaag gagagataga ccatgaaaaa aattttacta
     3181 gtatgttcag caggaatgtc cacaagtttg cttgtgacca aaatgagaaa ctccgcaaag
     3241 gaaaaaggaa ttgaagtaga aatagatgct cttccagttg cagaggcaag tacggccgtt
     3301 gataatgtcg atatcgttct tttaggacca caagtgcgtt tccagaaagg gaccgttgaa
     3361 aaacttgtaa aaggcagaat tcccgtagaa gtgatggaca tgcggctgta tggaatgatg
     3421 aatggaaaag cgattttaga agatgcttta caaaaaataa actaggaaag gaggctaaaa
     3481 aaaatgagtt ttatggacaa atttcaaagt gctatagaaa gattgttagt gcctttatct
     3541 acaaagttga acgctcaaag acacatatgc gcagtaagag atgcttttat tttatcattc
     3601 ccattaacga tggcaggatc attgatggtg ttattgaata atgttttctt gtcagcagat
     3661 ggattcatgt ttaaaatctt gcaattcgat aaactttttc cgggtattgt taaattccaa
     3721 gcagtgttcg ctccagttgt taaaggttca gctgatattt tctcgattct gattgtgttt
     3781 ttgatagcca gaaaccttgc gaaactaatg gaaggcgatg atttgttgac aggtttgacc
     3841 gcaatctctg tttatttcat catctatcct gattatttcg ataatgaagg agtaaattat
     3901 ctaacaaatc aatatatggg cgcacaaggg ttgtttgtag caattttggt tgggctcttg
     3961 gtcggcgaac taatgtctag attagcaaaa tcagataagt tagaaattaa aatgccagaa
     4021 caagtacctc cagcagttgc aagatccttc aaagtgttga tcccgattat catcacgacg
     4081 gttttcttct ccgtcctcaa ctactttgta aaaatggctg caccgggcgg attacatgaa
     4141 ttaatttata acatcattca aacaccattg accagaatga gtcaaagttt attctcagtg
     4201 attattttag cattattatc tcaattgcta tgggctatgg gaatacacgg accaaatacg
     4261 attgcagcta ttcgagatac catgttctcg gaagccggaa acgcaaacct cttacattat
     4321 gctgaaagcg gtactacatg gggagcgcct tttccgataa cgtacagtgg tttagctact
     4381 gctttcgctg aatatggcgg ttcaggagcg acattgggtc taatcattgc tatattgatt
     4441 ttctcgaaaa ataaggaatc gaaaagtatt ggcaaacttt cttttgtacc cggtttgttc
     4501 aatataaacg aaatggttat ctttggttta ccgattattc tgaatccgat ttacattatt
     4561 ccatttatta ttgcgccact ggttaacatt atgattggtt ataccgcagt tatgattctt
     4621 aagattatgc cgccggtaac gattggtatt ccttggacaa caccaggacc attgatgcct
     4681 ttcttgggta caggcggaaa tatcgtcgca ctgtttgtcg gattcatctg tcttgcggtg
     4741 agtgtattga tttactcgcc gtttgtaatc gcagcgaata aagcagcgat tattgaagat
     4801 gaaatggaga atgaaataca gtcacaagaa gtagtagaat cgatttaaaa aagctgcagt
     4861 acgctgagaa caaaccgaaa gattcaagat aggagattta cacatggaag gaattgaatt
     4921 aactgctttt agtataatca gtaatgttgg aatggccaaa tcattggcta ttgaagcatt
     4981 aagagatgcc agaaaaggaa agtacaatga agcagaaaag aaaatggctg aagcatcgga
     5041 atattttgtg aaaggccatc atgcacatac ttctttgata caaaaagaag ctgctggcga
     5101 aaaagttgaa ctttcgttaa taatcatgca cgcagaagat caattgatgt cgacggagac
     5161 aactaaatta ttaatcgaag aaatgataga aatgttcaaa cagttaaaca ataaataagt
     5221 gaacatgaaa gagaaaatat tagacgaact agaattgctt gcttacttta caggcagaat
     5281 ctgatgccag cagcaaaaca tagagcaccc aggatgaaat ataatcctgg gtgccttatt
     5341 tttagaacaa aacaccgggg tggtagtttg ttctgcggtt gaaggcattt gctgatggtt
     5401 tctgcatgaa aggtattgcc aaaagtctcc ttttgctgga atcctgaagc aggcagagtt
     5461 tttcgaaaaa cagaaaaaga ttgaaaaaaa tggacaaata atatagtgtt gcagtatgaa
     5521 cacgagttta cgaacgaaag gagatgcctt tatgatgaat agtactgaaa ttgcaaaaat
     5581 tgccggagta tcacgcagta ctgtatccag agtgataaat aattattcaa atgtctcgga
     5641 agaaacaaga gaaaaggttt taagagtaat taaggaacat gagtatgtgc cacatgcttc
     5701 agcaaggatg cttgcaggaa gccaaaataa ggtgatcggc ttatttatta tagacctgat
     5761 acatgaagaa gttggactaa aaaatagaat taccaaaagt ccgtattatc tagaatttac
     5821 tagttcagtg atagaaacag caagtgagat ggattacctg gttcttgtcc atattataca
     5881 tagcgtcgca ggctatgaaa aaattaaaga gagtttttat aataagacaa tttctggtgg
     5941 gattttcata ggccagaatg acgacgatga atcgataaaa acaatcattg atagagggta
     6001 taaagttgtg ctgctcgatc aatcaatcag acctgaagat gccacctata accagtgcat
     6061 gatagtgaat gcggacaatt acaacggcgc ttattgtgcg acaaaatatc tcatagataa
     6121 aaaacataaa caaatcgcac atattacagg cggtacggtc aaattctctt ctaaagacag
     6181 gataagagga tacaaaaaag cattagaaga tgcagggata gaagtaaata agaacttgat
     6241 tgtaaatagc gaatttgtgg aaaatgctgg atatgaggct gcgaaaaaat tatttaatgg
     6301 aaatagtgag ttttcggcta tttttgccag taatgataag atagcatttg gagcaatcaa
     6361 agctattcaa gaaagaggtc tcaaagttcc agatgacatc tctgttatag gatttgatga
     6421 tattgaatcg tctaaatatt ttaatccgcc attgacttct atgaaaatga agttaggaga
     6481 aatggctgat atcgccacaa agtcgattgt tgcatcgatt gaaggtgaaa cgaagccatc
     6541 tataaatgta gtacctgtct cattagtgga aagggagagt tgtgggattt tgcagtagca
     6601 aaacttgctg aattagttgg agctgattat ttgattattt tgattgaggt agacaatgtc
     6661 tgtgtctatg tcgacttgaa aagtaaagta cagcgcccaa aaatgctctg tactttaaat
     6721 ctcagcaaaa acaggaatat ttcccaaaca tctccttctt tgagtaatta tacaaaaatt
     6781 atgcagactt tcttttttca tgataagata atttaaaaag tttttaaacg atttctcctc
     6841 ctctttttct tatatcgggg gagcatattt ttttgtagac taggggatta taagttcagc
     6901 catcaatgtc tagctcccaa gtcctgacct agtgaaaaaa agataaattt gccccattgc
     6961 gcgcttcgct gctcattcag ggtcaaattt cctatttttt cataggtcaa ggcggacttg
     7021 tccgcttttc ttatattaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc
     7081 tttcttctaa tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag
     7141 ggattgcaaa aattgaagca acttttgtcg aagctcaaaa aaatatgggc tgttttacct
     7201 gaacattctt tgaagcaacg cattggtaat aatctagctt tgatttatag tggagctctc
     7261 ctaattcaag atatactggg ctatccttta cgattagagc atatacagac ggtgcttatt
     7321 aattcgtacg aacagttaat cgtagatact agtttaggaa gtgagcaaac ggtatttgac
     7381 cgagtgaaaa cattgttggt gatgaatagc cataaatttt taacaaacag tgacaacaaa
     7441 catacgccca gtacgatttg gggaaaagta tcaattaaaa aaaatgggga tattaaggta
     7501 aatatttttc ctatcccgtt tgaggacttg ttaaggcgag aatttcaagt aaaggatcta
     7561 acctatcttt ttagacaatt gcttgattcg ggtaatatgt gtgcagaaaa agggagaaga
     7621 acgaaacgta ttcacatgaa tcgtcaagtt cttccaacat acgaaattct attaccttct
     7681 tcactcaaat cgtattttcg attgtaattt agaataaacc aaagtatgaa agtgaggcac
     7741 catgttatta agcaactgaa agatattcct attccaatgc ccgctatttc agaacaacaa
     7801 aaaatcggca ctttcttcaa acaactcgac gacaccatta cccttcacca acgtaagtta
     7861 gatttactaa aagaaacgaa aaaaggcttc ttacaaaaga t
//
LOCUS       DAZKCN010000110         7896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000110 DAZKCN010000000
VERSION     DAZKCN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.113"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(178..696)
                     /locus_tag="ACG8K3_002345"
     CDS             complement(178..696)
                     /locus_tag="ACG8K3_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP9146168.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(698..1315)
                     /locus_tag="ACG8K3_002346"
     CDS             complement(698..1315)
                     /locus_tag="ACG8K3_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP9146169.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1312..2166)
                     /locus_tag="ACG8K3_002347"
     CDS             complement(1312..2166)
                     /locus_tag="ACG8K3_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9146170.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2163..3074)
                     /locus_tag="ACG8K3_002348"
     CDS             complement(2163..3074)
                     /locus_tag="ACG8K3_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9146171.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3115..4449)
                     /locus_tag="ACG8K3_002349"
     CDS             complement(3115..4449)
                     /locus_tag="ACG8K3_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9146172.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4657..5487
                     /locus_tag="ACG8K3_002350"
     CDS             4657..5487
                     /locus_tag="ACG8K3_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFP9146173.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5731..5980)
                     /locus_tag="ACG8K3_002351"
                     /pseudo
     CDS             complement(5731..5980)
                     /locus_tag="ACG8K3_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6171..6365)
                     /locus_tag="ACG8K3_002352"
     CDS             complement(6171..6365)
                     /locus_tag="ACG8K3_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146174.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6870..7055)
                     /locus_tag="ACG8K3_002353"
     CDS             complement(6870..7055)
                     /locus_tag="ACG8K3_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146175.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2606 a         1528 c         1329 g         2433 t
ORIGIN      
        1 aaaacaccaa ttatacaaca tttattatta aaattaaatt ataaaatttt ataacgttct
       61 catttaacaa aacttaagtt attaagacaa atgaataaac aactacgctt tcctcgaaac
      121 aaaaaaataa agcacaaatg cgcatttacg cactcatgct ttattcgtac aattaattca
      181 gataacggca gcgcatagga tgccaataac acttcctaaa atagctcctc ctaaaatgtc
      241 agtaggataa tgtaaaagta aaaaaatcct cgaataagcg atcaaacaag caaataaaaa
      301 ggctaatatt cctgcaactg gacttaattc gaacaacgca gctactgata aaaaggctag
      361 cgctgtatgt cctgaaggaa aagaagaagt cttcggttta cttgtgtaaa ggacaagccg
      421 acgatttttt tcataaggtc ttttccgtct tgtcgttttt ttgataaaaa gcaaaccaac
      481 aaaaaaatag atcaaactgc cgataagaat ggctagacca tattctctag tatcaggtat
      541 caagactaaa acagcagcaa ggagcaacca aataaaacca ccattagaga tattactgat
      601 aaatttcaaa aaattgattt ttcgactatc cgttttccgt tgcttggcga acttgttgat
      661 aatactatca tcgattttag caatcaaacg caacatgtta ttcctccttt ttaagaacct
      721 tttcttgcgt acgatggata aaaagcagga cttgaaaata aaacaagaaa ctgaaaccta
      781 ataaagcaaa atagattggt cctgcaaacc agaatgcgac aagaaggatc aacgtgcctg
      841 ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa gaaaaatgca gtaatccaag
      901 aagcttcttt ttgttccagt tgaagcaaga ttttgtaact aaagagaagc gcgcttaata
      961 gaagcaaaaa caacgtagta tacatcaatg tatagccaat tcccccaccc caatagcgga
     1021 aaatctgata gtcgatcaat agaagaagaa aataaacact tgctaaacta tcttgctttc
     1081 cataaagaga gaagttctca cgaaactttt tccagaatag tgaaccgagc tttttctctt
     1141 catttttttt gtaatgataa atcatataca gaactgttcg acttgcagtt cctaatgtga
     1201 aaacaggcaa gacgcacagg aaccatagta gctgtaaatg gataagacgt ggaatccatt
     1261 caatgatttc taagaaataa tcattggctt ttttaccaat cgatcgattc atcatccttt
     1321 tgaacctcct gtcaaatcca tcccttcaat gaaataacgt tgtgcaaaaa agaacagcaa
     1381 aatcgtcggg atcaagacaa gtgttgctcc tgccatcaga taattccatt gtgtcgttgc
     1441 ttggttctga aaaacttgca agccaatctg taacgtgtat ttttcttgat cttggatata
     1501 taacagagga cctaagaaat cattccaagc tgcgttgaag gaattgatcg caatggtaat
     1561 caaggcaggt ttcgtcaatg gaagcatcaa atgactccaa atatacaaat gattcgctcc
     1621 gtcgatttca gcagcttcga tcaattcatt attgatcgat aagtaaaatt gacgcatcag
     1681 aaatatattg aacgcattgc cgaagaacga tggaacgatc aggggcaaat aagtacctac
     1741 ccagccgatc ttggagaata atacatattg agggatcatc gttacaaacc ccgggatcat
     1801 catcgtagaa agaaccaaag caaacatcag ctttttccca ggaaaatcaa gcttcgcaaa
     1861 tccataagca ataaaagaat ttgaaaggac attaccgatc actactagca cagtgatgaa
     1921 taatgtattt cttgcatagc gccaaaaagg aaaggcggca atcgtatcca gatagttttc
     1981 ccatacgatt ttttctggga aaaaggtcgg aggataacga acgatttctt ccattggttt
     2041 caatgaagta aacaccatcc aaagcaatag aatgatcaaa atgattccta ttactgtcat
     2101 gaggacataa cttagtattt tttttgtttg ccgattcaca ctggtctttt tgaaataatc
     2161 catcatttgc cgtcacctcc agaataatag acccacttag gtgcaagctt caaattgaca
     2221 aacgtcagaa ccaacacgat cagaaacagg atcattgaca tagccatcgc ataacccata
     2281 tcaaaagcta cgaacgcctt gttccacata tgcagattat aaaaaagcaa agaatttgca
     2341 ggtccgcctg ttccattttt cgtcatgaca taagcttctt gaaaaatttg gaaagaaccg
     2401 atcgtagagg tgatcacatc gtaaaaaata ataggagtga ttaatggcaa agtaatactt
     2461 ttgaactttc cccaaacgcc tgctccatcg atttctgctg attcatataa agatctagga
     2521 acattttgta atgtagctaa gtaaagtagc attgctcctc cagaactcca tattttcatc
     2581 aaaatcaacg caggtttcgt ccattgtgga tcaaacagcc aagctggtcc agggatatgg
     2641 aaccagccca gaaatgtgtt gatcaatcct gcagatggag aaagcagctg catccataag
     2701 atgtagactg ctactcctga taagactgcc ggtaaataga aagctgtacg gaatacactg
     2761 attcctttga ttttttgatt gagtactgtg gctaaaaaca cactgaaaat cgtcgtcagc
     2821 ggtacattga agatgacgaa ataaacggtg ttgtataacg atttccaaaa taaatcatcg
     2881 tgaacgaaca ttcttttgaa atttgctaga ccaatgaaat tcatcttgga tgtcaaatta
     2941 taatcggtga aactaccaat aactgaaaaa agcataggcc ctagcataaa taccaagaac
     3001 ccgataataa aaggcgagat aaataaatag gcccatcttg cttctttttt ctttcggata
     3061 gataaacgct tcaaattttc ttgcctcctc tgtacctaga cagtcaaaaa tggattattg
     3121 attttgcttg actaagtttt ctactgcttt ttgtgcatct gccaatcctt cttttggtga
     3181 ttttgttcct aaaagaatct ggtcaatctg ttcattcact aatgtcgtgt aatccggagc
     3241 atctaaagga actggtgtga tcaccgtatt tttaaagttt tcgtccgcca tttgatagat
     3301 catttttcct ttatccgtta ggttgtcatt attcactaaa ttctcattgg ctgtcttgct
     3361 cgccatgata tcaaaacttt tttcgccgaa tttttcttgt acttctggag aagaaagata
     3421 tttgatgaat tcataagagg cctcaggatc ttttgctccg tatggtactt ccaagacgaa
     3481 tccgccaccc cacgaccaat ggcctgaacc ttcttctttt tctgggagct gcatcacgcc
     3541 aaaatcaaaa tcatccggtg cattttccat caaactagag taataattga tattttgggc
     3601 acgcatggct actaatccgg aaataaatgg atcagataca ccagacccaa attcggcttc
     3661 taatttattg atcgtatttt tcccataata atcttgccag tcttggatcc actccaaggc
     3721 ttccacttta tttttagtat caatcttgac tttttcatct ttgtcaaacc aactagtccc
     3781 gttatctgcg tttagcgccc atacatcagc ccctaaattc cataatggat aaaaaccgat
     3841 gcgtgtaaat tcttttcctt cttttttatc tagtttacgt gctgtttctt ctaattcatc
     3901 ccaagttgtc ggtagatctt gttcactgat tccagcttct ttaaataact ttttattata
     3961 aaaaatgact tgtgtatctg tattgaatgg gatcgcataa ggatcacctt catattctac
     4021 tgtatcccaa agctgcggat aaaaatcctt agaagtgtct ttttctacat atttggagag
     4081 attagttgct tgctgtgctt ctgctctttg ggcaacggaa tttatgtcct gtacaataac
     4141 atcgggtgca tttccagctg ttattgcaga taaagattta gtccagatgt caccccatgg
     4201 ctgatactta taatttacat ggatctcgtt ttgtgattga ttgaaatcct caatgatttc
     4261 ttcgatagct tcttttcgtg caccggatcc ccaaaaggac cagaaatcaa tattttttct
     4321 tccatcactt gttttttcag aagaatttgc tgcacttgaa ctggcatcgc ttgcattact
     4381 tgatccgcat cctcctaata aaaacaatgc tgaacttgcc agcaaccctg taaacaataa
     4441 ttttttcata ttttacctcc aaattttaat gtaaccgatg ccggttttaa tataacgata
     4501 tcattttgtt ctgaaaataa caatttataa ggtatgattc taaaaaaatt attaatgata
     4561 ccaatacttt atataattcg cgagcgcttt ctttgaattt tttaatcttt cgtagcagaa
     4621 tgagaatgta ttaaaaatag attaggagga attttgatgt ttagacacca gaaagaattg
     4681 cagtttgaag caaaaccaga tcgtccgaat ccattaatcg cgaaatatct gcaggagctg
     4741 atcggcggcc aatatggcga gatgagtgta gctatgcagt acctgttcca aggctggagc
     4801 tgccgaggag aagaaaaata taaagatatg ctgatggaca ttgcaacaga agaaatcggc
     4861 cacgtggaaa tgttggcgac aatggttgct cgtttattag agggtgcgcc tgttgaagat
     4921 caagaagcag cgatgcaaaa agatccaaca atcggtgcca ttatctctgg aatgaatcca
     4981 cagcacgcga tcgtagctgg cttaggacct cgtcctgctg acagcgtggg caatccatgg
     5041 agcggtgcct atatcattgc gagtggcaac ttgttagcag actttagagc taatttaaat
     5101 gccgaatcac aaggaagact tcaagtagca cgtatttacg aaatgatcga tgatcgtggc
     5161 gtaaaagata tgctgtctgt tctccttgca agagatagct atcatcaaaa tctatgggaa
     5221 tgtgcagtga aagaattaga agaaaaagaa ggttcaatct tagtcccaag tactttcccg
     5281 cgtgacaaag aacgaaaaga cattgcttat gacttctata acttctcaga gggagaccaa
     5341 agtagtgaag gttcttgggc tagtggcaaa gcactggatg gcgaaggcga atatcaatgg
     5401 ttgaaagaac ctaagatcga aggcgctgca ccaaaattag aaccagtaac accaaaaatg
     5461 tatgggacac ctccattcaa gaaataaata tctacccgta gaaacacccg ttttttgttc
     5521 cacacgcttt gtgagcaaaa ctgactaaag tcattaatca taaccaccct tcaaaagggt
     5581 ggttttctct agagctataa gctcaaatca ccggccagcg cctaaagacg ctggctttca
     5641 tattttatgt tcaagccata ttgcctttgc cagttttgcc cacctcttaa aaggtttatg
     5701 tactactcct tagcccttaa aaggatcgtc ttactctcgt acactcagct tatctaacgc
     5761 actatcatgt ttttcttgtt cttgaatgta ctttttgatg gtcgcttcat tttgtcctac
     5821 ttttgtcaca tagtatcctt ccacccaaaa gtgtcgatac ccaaatttat attttagatt
     5881 tgcacaatct cttgatgatc tcctgaatat tggctcgata ttgattataa attatttttc
     5941 ttctatactt tggtgtgaat acaatgtggt atttacacat ctactttgta cgggctaaac
     6001 tatttccctt tattgctcca cccgcatagc aagtggtttt gtatttcaat cgctcggcgc
     6061 gctcaattag gtctaaagac aataaatcta aaaacccgct gcttcactga cagaaacaac
     6121 gggttttcag aaaaaaatga aatgaaaaag atctcttacg gcaatctatt ttattttttt
     6181 gattttttat aaatggaaaa tgcttcaaga agtccaagaa ataaagccgt ataaaaacaa
     6241 gaacctatga aatctttatt tattagatat tcaacagaaa tcccgataac actagccaac
     6301 aatatattta tcagaagtgt aaaataagga tgctttttat agaatagaac tagtgttgtt
     6361 ttcatagact ctcctttctt catatttcca attaatcaca aattcacggc attactagaa
     6421 aaattaaagg ggcgcagaag tggaaaaata ctcttcgact ttccctacaa accaggccat
     6481 cttgtgttta ataactatac caaatctaag gtacctctaa atctaaattc catttgtggc
     6541 ggtgacctcc aatttttaac tcttctatct cccatccagt atacataacc ttgatacttc
     6601 gaccacttat atatcgaaca cacaaatacc tatccattgc aggagtacta cctaacttag
     6661 ttgctacttg catctaaata acagctgaaa agataggact tactcttagt aaagtatctg
     6721 aactattaat ttttaaacta tcatttttat cctcaactac atgcacatct gttgcaactt
     6781 cactttctgt ctcatccaga taaatgatta aggaatcggg aattgcaata tcttcagctg
     6841 acgctggaat agttgagcta aattagtcat catatcttta ttataaaata accaacagca
     6901 aaaaatacaa tgaaaaacaa acttggaact aatgtatcat taataaatgt tgaaaaattc
     6961 agcaactcat tactgtatga ttttagcagc ccaaccaaca tataccctat aaaagctaat
     7021 aagatggtta ggaatccttt agaattttta ctcattcata gacacttcct taaataactt
     7081 ttattataga aggtattgta aataataata ttgacaaaaa gtgactcaaa ataaaaaaaa
     7141 acttacatta tactggaatg aactgtattg atagtgtacc taaaagctct actgacataa
     7201 gatcgtgtaa tcggtaaagt aattttatta attatacggg atgtaatcaa ttagagaata
     7261 aactaatcat aaaaggtact tttacattca taattgtcgc tttgagattc tactttggtt
     7321 cactatctga cccaattgca cattttcgat cattacaagt attggtgtcg ttagaacaac
     7381 taaataaaaa ctagcggtag atttttcaga atctatgcta atttctgtat ggatgttcca
     7441 gtgatggcaa atttctcttt tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta
     7501 ttcagttaaa ctatagaaat tctttcctag acagatcttg atcccaaatt ttacgttttt
     7561 gagcatcgat atagtttaaa atgacattac tcatatctaa agagtttaga ttttttagga
     7621 ctaaaactgt cagttgttct ttttcttctt tctgttaaag tattgtcgcg acttttcgta
     7681 tacgttaaga tatgtcgtga aaaaacaagt tttgtgacac aaaaaaacga atgacttgag
     7741 gaaatcacct caatcattcg tttaaaatgc tatatattct ttagtttaag atacggaaat
     7801 taatgccatc gaccacacta gtagaatgaa cgacatttac ttggcctttt tttaatttcg
     7861 ttatgtttag catataaaaa tattatagag tttact
//
LOCUS       DAZKCN010000111         7875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000111 DAZKCN010000000
VERSION     DAZKCN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.114"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..788
                     /locus_tag="ACG8K3_002354"
     CDS             87..788
                     /locus_tag="ACG8K3_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP9146176.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1360..2376)
                     /gene="tsaD"
                     /locus_tag="ACG8K3_002355"
     CDS             complement(1360..2376)
                     /gene="tsaD"
                     /locus_tag="ACG8K3_002355"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9146177.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2373..2837)
                     /gene="rimI"
                     /locus_tag="ACG8K3_002356"
     CDS             complement(2373..2837)
                     /gene="rimI"
                     /locus_tag="ACG8K3_002356"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9146178.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2844..3386)
                     /gene="rimI"
                     /locus_tag="ACG8K3_002357"
     CDS             complement(2844..3386)
                     /gene="rimI"
                     /locus_tag="ACG8K3_002357"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9146179.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3370..4092)
                     /gene="tsaB"
                     /locus_tag="ACG8K3_002358"
     CDS             complement(3370..4092)
                     /gene="tsaB"
                     /locus_tag="ACG8K3_002358"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9146180.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4283..5263)
                     /locus_tag="ACG8K3_002359"
     CDS             complement(4283..5263)
                     /locus_tag="ACG8K3_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146181.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5287..6771)
                     /gene="galT"
                     /locus_tag="ACG8K3_002360"
     CDS             complement(5287..6771)
                     /gene="galT"
                     /locus_tag="ACG8K3_002360"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP9146182.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6783..7772)
                     /gene="galE"
                     /locus_tag="ACG8K3_002361"
     CDS             complement(6783..7772)
                     /gene="galE"
                     /locus_tag="ACG8K3_002361"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9146183.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2207 a         1695 c         1391 g         2582 t
ORIGIN      
        1 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
       61 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
      121 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
      181 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
      241 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
      301 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
      361 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
      421 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
      481 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
      541 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
      601 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
      661 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccggac
      721 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
      781 gagaataacg cgaacaaaaa taagcagtga ccctgatatt tatcagggtc actgcttatt
      841 ttcctcccta aaacttctga aagatccggc agttttagat cactgggcaa gactcaaaga
      901 ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgatagatta
      961 actagaggaa gcgtcttgtc ctcatccgac ctagattatt cgatattcct cgacaataaa
     1021 cgattaatca gtgagggcaa tcagaggaac ggataatgat gaataagctg taaaaaatag
     1081 ggctttgaca aaagcgtgtc acagccctta tttcgattaa acggtgtgta cctgtcaact
     1141 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
     1201 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
     1261 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
     1321 tcttattact tgatgcaggg cagatttttc acaacctctt tattcagata aaaccaagct
     1381 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
     1441 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
     1501 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
     1561 tacacctcca gctacaatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
     1621 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
     1681 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
     1741 aaaactgaaa tcataattat cttcatggat catcgctcgt ggaaaatggt aattatcttt
     1801 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1861 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1921 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1981 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     2041 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     2101 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     2161 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     2221 gcggctagct acttcaggaa ctacgccacc aaaacgttta tgactgttta tttgagaagc
     2281 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     2341 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     2401 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     2461 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     2521 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     2581 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     2641 attactttcg agtatccaat aaagccacag atctggttat cttcaactaa aagcagataa
     2701 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     2761 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2821 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2881 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa acctaacttc
     2941 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     3001 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     3061 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     3121 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     3181 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     3241 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     3301 cgatcgattt gttcgattgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     3361 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     3421 tttttcttct gcttctaccc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     3481 tggttcagct aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     3541 aactgtatta atcatcgcac taggcaatat ttcctttatt tcatagaaga atttgtgaca
     3601 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     3661 agcagaaata tgtacatctt caagtacaat ttctaatcta ttttctttcc agcggtatgc
     3721 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataattt tatccacacc
     3781 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3841 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3901 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3961 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     4021 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     4081 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     4141 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaatgat acatgattat
     4201 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcacttcat
     4261 ttccccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
     4321 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
     4381 ctagctctcc catccattcg gtataaactt ttacagtaga aagagagggc gttacatatt
     4441 tcgctacact gacatcattg aaacccatca cagaaatatc ttctggtacc actaaacctt
     4501 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
     4561 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
     4621 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
     4681 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
     4741 caagcatagc gatatgttgc gctttatggg agacaaaatg ttcaatgact ttttgaacac
     4801 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
     4861 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
     4921 gtgcgatcgt tccatctgtc ggaagatcac taatggaatc ccacgtttct ttcaataaag
     4981 taacgtttgc ttcttctgcc tttttttcga ttcctagacg aatagataaa taataaagat
     5041 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
     5101 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
     5161 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
     5221 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
     5281 ttacgtttat aaaattgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
     5341 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
     5401 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
     5461 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
     5521 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
     5581 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
     5641 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
     5701 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
     5761 ttcatccgaa tatgctttcc acttttccaa tactgagtta gctgcttcaa tcaacaattt
     5821 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
     5881 ttgcggataa tctgctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
     5941 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa tcggcaagtc
     6001 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
     6061 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
     6121 gtaataagca taaggagaat attggaatcc ccagctttca ccgtctagat tcattcgaat
     6181 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
     6241 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
     6301 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
     6361 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
     6421 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaaaaa
     6481 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
     6541 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
     6601 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
     6661 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
     6721 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
     6781 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatt
     6841 gctttgatat ctgtgtattc aggtttccaa cccagaacct ctctcgcttt ttcacttgaa
     6901 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
     6961 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
     7021 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
     7081 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
     7141 tcgtctccat aaacagccaa tgcttttctt tgaccaagag ctacttgtaa aatgattggg
     7201 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
     7261 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
     7321 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
     7381 tctgtgattg gagactcttt tggttctccg taagttgctg cagtagaaga gaacacgatt
     7441 ttattcacat tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
     7501 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
     7561 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
     7621 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
     7681 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
     7741 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
     7801 gtgtttgttg attatatata atttaaaaaa acgaagttta cttttatggt gaaaacaaac
     7861 aaagtaaaat ttcgt
//
LOCUS       DAZKCN010000112         7518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000112 DAZKCN010000000
VERSION     DAZKCN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.115"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..346)
                     /locus_tag="ACG8K3_002362"
     CDS             complement(56..346)
                     /locus_tag="ACG8K3_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146184.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(464..724)
                     /locus_tag="ACG8K3_002363"
     CDS             complement(464..724)
                     /locus_tag="ACG8K3_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146185.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(861..1274)
                     /locus_tag="ACG8K3_002364"
     CDS             complement(861..1274)
                     /locus_tag="ACG8K3_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP9146186.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1730..2215)
                     /locus_tag="ACG8K3_002365"
     CDS             complement(1730..2215)
                     /locus_tag="ACG8K3_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146187.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2354..2569)
                     /locus_tag="ACG8K3_002366"
     CDS             complement(2354..2569)
                     /locus_tag="ACG8K3_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP9146188.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2793..3593
                     /locus_tag="ACG8K3_002367"
     CDS             2793..3593
                     /locus_tag="ACG8K3_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9146189.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3612..5480
                     /locus_tag="ACG8K3_002368"
     CDS             3612..5480
                     /locus_tag="ACG8K3_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP9146190.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5473..5964
                     /locus_tag="ACG8K3_002369"
     CDS             5473..5964
                     /locus_tag="ACG8K3_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9146191.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5952..6506
                     /locus_tag="ACG8K3_002370"
     CDS             5952..6506
                     /locus_tag="ACG8K3_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9146192.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6524..>7518
                     /locus_tag="ACG8K3_002371"
     CDS             6524..>7518
                     /locus_tag="ACG8K3_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9146193.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2672 a         1204 c         1432 g         2210 t
ORIGIN      
        1 ctttcaaaaa tatgttttgg aagttgggac aaacgagtta atacatttta atacattatt
       61 ttttattcaa aaaaaatagc acaatggcaa agatcgtaag agagatcata agccccaatg
      121 aatgactaaa aagaaaagca gaatcacatg ccatatcatc ccatatttct tcaagaaaat
      181 aaataagcgg gaaaaaggga aggaatagta gaacatcgat ccagcaccat ttttttcttc
      241 ttttgaaata acataaaaca ataaacatga acgtgcaggg aagtaaaaac aaagctttac
      301 tcataactaa caaaatacca gctcctttta tttttttctc ccccacattc aaagagtaac
      361 aatggtatgt acttaataat agaaaaatgg atcaacttgt atgaataaca gctgaaaaga
      421 tctgcaccaa ataaaaaact tccagccaaa actgactgga agtttatttt tttctgaaat
      481 attctaacac aaatatgaaa aagatggccg aagctaataa gccgatttcg tagcaaaaca
      541 atgagatacc agagtcgtaa taataaaatc ctctaatctt atggataaaa tagatcatcg
      601 gacaagcaga taagagtaag tatttgttag gccttttgaa taagtaaaag aaagcactgg
      661 tcattatgat aaatggtatc caaaacaaaa gtttacttaa gacaatcaaa tgatcaactc
      721 ccatattttt taccaagaga gatcataaca tctttatcgt aaaaacaaaa tatttttttg
      781 ctcattttga aaatttaaac atactcgttc ttatccaaat atataaaaat cgcccctcat
      841 tcatcgagag gcgataagtt ctaatttaac tcaatttttt ttgtttcttt tggctgataa
      901 gctgttttag gtaataccaa tttcagcaca ccgtttttaa aggttccagt gattttttct
      961 tcatcaacat taggaagata gaatgaacgt ttgtaagaga ccgaatgtct ttctttccgt
     1021 acatagttgc catcttcttt atcttctgta tgggactcat gattggcgga gatcgtcaaa
     1081 gtattatctg aatattcaac tgtcgtgtct tctttgtcac agccaggcat atcagcagtt
     1141 agtttgtatt ctttttcatt ttcgactaaa tctacttttg gataacttga aacacctaaa
     1201 aaatcattga atgctggact aaacacgtca ttgaaatcag ggaacatgtc gcgaatgcta
     1261 ggtacatttg ccatttttga tcatcctttc aataagtatt ttttatttat ccctatttac
     1321 gtaataactc tattttgaaa aaaatgcaag tataatggtt agctttgtga atatttcatc
     1381 taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa taataagaag
     1441 aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa aattgaatct
     1501 agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa gtagatacca
     1561 aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa tagctgtaac
     1621 gaattattag gttaatgcaa atgcttattt atactccgta taactttctc agaaaaaatg
     1681 ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat caaaatggtg
     1741 ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa aaaccaacgc
     1801 ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa taatctcgat
     1861 caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa cggtgggcaa
     1921 taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca gtgctttctt
     1981 gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta tgttgcgaat
     2041 ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta gttgaaccaa
     2101 cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt ttatatccac
     2161 aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta tgcatgatag
     2221 ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa aagcagatgg
     2281 tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata acttttttaa
     2341 taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg aaaaatttca
     2401 agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa atagttgttg
     2461 aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac agccagttca
     2521 accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt agaattctcc
     2581 tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat ataacgctca
     2641 ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca ttagtacaca
     2701 ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct tttatactgg
     2761 atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat attgcaggaa
     2821 aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc gaaaatttgt
     2881 tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata cagcatgaaa
     2941 acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa gggatatata
     3001 aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga ataaatattc
     3061 cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca gaacaagttt
     3121 ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa
     3181 gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg
     3241 aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac agttttactc
     3301 gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga
     3361 tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct tatacgagag
     3421 gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc cctctaggaa
     3481 ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca gaaagatcaa
     3541 gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga taaaggatag
     3601 gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta ctagtcactc
     3661 acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa
     3721 aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa
     3781 acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc atgcaaggaa
     3841 aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat ttactaaata
     3901 ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa gtcggtgtca
     3961 gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat gattttgatg
     4021 taaagattac aggcacacca aataaaggac taagaataat aggagatgag ctagaattac
     4081 gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca gaacagtttc
     4141 tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt aaacaagaaa
     4201 tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt aactatctgt
     4261 tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa ttcaatgaac
     4321 tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa aaagatttta
     4381 tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa gctaactctg
     4441 atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat ctcattgatc
     4501 tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg ataaatcggg
     4561 taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa aagtatcctt
     4621 tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg caacataaga
     4681 tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt cgatatcaaa
     4741 aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa ggaacagcat
     4801 taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata acacatgttt
     4861 cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt acaacgattc
     4921 cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt tttgatgact
     4981 cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg tttcattcta
     5041 ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat
     5101 tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa aggatcttga
     5161 aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct cacacaacca
     5221 accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa ggtttagcag
     5281 aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg acggctgaag
     5341 ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa atgggaatag
     5401 atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc aaaagtaaag
     5461 gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc agcttatgtg
     5521 ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata tagcgaactg
     5581 cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc aggcacgatt
     5641 gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt
     5701 acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat ccattacatc
     5761 gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc tttagaaaat
     5821 gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca accttcattg
     5881 ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa agaaaaaagg
     5941 agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg ctgttccaaa
     6001 caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc ttgatgctga
     6061 tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg aaagtagcca
     6121 aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta tctgctttta
     6181 tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac tacaaaccaa
     6241 gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc ccccatatta
     6301 atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc ttagggctaa
     6361 attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac tttattggtg
     6421 gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc gttcttagca
     6481 aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg gtgatattaa
     6541 gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga caaaagataa
     6601 acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag ataaaataga
     6661 agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat
     6721 cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc ctaaaaaagg
     6781 gataccaacc atcaatatcg tttccacagg aaaaagtggc ccattagcac aatatatcac
     6841 tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa tggaaactga
     6901 tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat atgatacttc
     6961 taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa ttggaatggg
     7021 cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag atacgatgat
     7081 ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga tacaaggttc
     7141 tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg cagttggatt
     7201 aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg gtccaggggc
     7261 agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag gaaatattcc
     7321 tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat gtgattttat
     7381 tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag gtgtaccatc
     7441 agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt gggtagcaag
     7501 ttttggatta tatagctg
//
LOCUS       DAZKCN010000113         7494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000113 DAZKCN010000000
VERSION     DAZKCN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.116"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8K3_002372"
     CDS             <1..649
                     /locus_tag="ACG8K3_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9146194.1"
                     /translation="YKINKITHQENRQKNLIVQNLNYGTNQYSVDNSKFELCDTSMKT
                     RDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENL
                     LKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYF
                     VDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            725..943
                     /locus_tag="ACG8K3_002373"
     CDS             725..943
                     /locus_tag="ACG8K3_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146195.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            930..3608
                     /locus_tag="ACG8K3_002374"
     CDS             930..3608
                     /locus_tag="ACG8K3_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFP9146196.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3599..3796
                     /locus_tag="ACG8K3_002375"
     CDS             3599..3796
                     /locus_tag="ACG8K3_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146197.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3800..4411
                     /locus_tag="ACG8K3_002376"
     CDS             3800..4411
                     /locus_tag="ACG8K3_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP9146198.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4396..5787
                     /locus_tag="ACG8K3_002377"
     CDS             4396..5787
                     /locus_tag="ACG8K3_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9146199.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6003..6317
                     /locus_tag="ACG8K3_002378"
     CDS             6003..6317
                     /locus_tag="ACG8K3_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146200.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6310..6804
                     /locus_tag="ACG8K3_002379"
     CDS             6310..6804
                     /locus_tag="ACG8K3_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146201.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6872..7186
                     /locus_tag="ACG8K3_002380"
     CDS             6872..7186
                     /locus_tag="ACG8K3_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146202.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7285..>7494
                     /locus_tag="ACG8K3_002381"
     CDS             7285..>7494
                     /locus_tag="ACG8K3_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9146203.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2888 a         1012 c         1438 g         2156 t
ORIGIN      
        1 ttataaaatt aataagataa cccatcaaga aaatagacaa aaaaacttga tagttcaaaa
       61 tttgaactat ggaacaaatc aatattctgt tgataattca aaatttgaac tatgcgacac
      121 atcaatgaaa acaagagaca aaaacaattt tgctgatagt tcaaaaaatg aactagaatt
      181 atataagtct tataaagata gtaaagatat taaagaatca cataatgatg atcttcttga
      241 atcactttca actaaaaata ataaattaga tgaagaattg atcgaaacat ttattgaaga
      301 aaatcttctt aaacaaatat ggggagaaag tattgtgaat tgcttaaaac tggctagcaa
      361 tagtgattat cgtcaattcg acaactggta caaaaaattc aaatatgcga ttagatctgc
      421 tgaaaaagaa caaaaaattc aactgaaaat tatctatgaa atttgtaata acaagtattt
      481 tgtagatcat gttagagaac agctatcaat gactttacgt gacttgatac gaagagcaaa
      541 aacagatcat cgtatcaaac aaaaagacga ttatatcttt gcttctctaa aaaataaagc
      601 gttagaacta attgaattgc aaatcagcga aggaatagat aagcaataag aatagaattg
      661 agtcaaatga aatggagggg tatctccact tcatttgaga actttgaata taggaggggt
      721 tattttgatt aaaaatcgtt taatagatca agtttggtgt ctagatcaaa atacagatag
      781 tctcgaatta atcgagacta tttgcttcaa cactatagtt ctcgatttaa gagctgaaaa
      841 tgataattgt gtaactcatg tcataattaa tcaaaaaatg gctcagaagc tctctgagag
      901 cctcagaaaa tgggctaagg gaggtaacga tgaaaataat tgaaaaaaag attgccaata
      961 aaaaaataaa ttggttggtt gttaattttc ttgttctgat agctatgatt atttttacaa
     1021 gtaatattgc tcatgcgagt attttcaatt ggtttgatga cacaaaaagt caaacagagt
     1081 ttttgaataa ttctctttat agaccttacc taaaaaagta caatggagat ttccttagtc
     1141 agttcgggat ttggctcgga tgggctgtgg taaaaggcat gtttactgtg acagattcga
     1201 ttcaaaatat gattccagat gtgctggatt tgttcaattt cattgaaagt acagggctaa
     1261 ataatgttta tcaatcagcc gtaaatacaa ttgtcattgg attaatgatc ctttcattga
     1321 tgtttgttgg ttacaaaatg attactggaa aaggaacagt ggatttaaaa tcggttggaa
     1381 tgaacgtagt gatgtctata gcattaatac ttttaatgcc aaccatgatt tctagtggta
     1441 ttcaatttgc taaaacattt tataaggact ctactacgat tacaaattca gatgacggag
     1501 tcgcttggtc attgataaaa caaggagtaa cagatttagc atatattaat aaaacagatc
     1561 agtactcaag cattgataaa acggaggatc gaaataattt aacacgaaaa aattttcaac
     1621 agacggatct cacacaagtg ttaacggata aagtgattga taaattggaa aaagaaaatc
     1681 caattgcgga caatcttcgt tatgaattag ttgaaaattc taataatgaa tttgttgcta
     1741 caaagttttc tgacaacttc ttatcaacat tttctgatag cttgaaatca ggctattacc
     1801 gataccaagc aaatctatgg gggatttcaa ttgggttgac agcgttagca atcgcctatg
     1861 tgttcagcgc gtttgtgatc attacagcaa tactagaact tgcatttaaa cgtgtgttag
     1921 gtgttcttgt ttttgcaaca gatattgaga caggacagcg ttcaaaagtt gttctatcag
     1981 atattctaca atgttactta actgttgggt ttcaaggatt tgggttatca atgtttgcta
     2041 tgtttatcaa ttttctgaac tcaggacaag gtatctcaac aaatattttt ataaaaacaa
     2101 ttgcttatat ctgtgccgtt tttgttttaa tcaaaggcag tggaacagtc atgagatact
     2161 ttggtgttga tattggactc aaagaaggct atgggcaact tgcttctgct tttggaatgg
     2221 gcgcaatgct gtttagaaaa ggatcaaatg gatttaatcg tgctaaagga agcggagaag
     2281 gagaagatcg aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa
     2341 ctggaagagc tttgggatat gcccatgaac gtggattatc cggtttagct tcagatggag
     2401 ctacaatggc tagtgaaaga gcaacaaaac catttaagag tatgcgtgat atagctaatg
     2461 ataccaagaa taagtttaaa gaaggtttag atgatggtac agtgtccgct attaataaaa
     2521 atagcaaacc tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattccg
     2581 tgccaacacg tctttcagat agaaaagatg gtgctaaagt tgaaaatgcc gatagaatta
     2641 tgtctagttc tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc
     2701 ctgcaagcgc aattcagcaa aaagtccaac aagatattga agaacgtaaa aatgctatgc
     2761 acggtcaagc taagtctgct gaagaattaa ttaatcagaa gcgtcatgaa gcgaaataca
     2821 cgcctgaagc aatgaatcgc gaggaaataa tgttaaggaa acgtgtagaa gggcggacag
     2881 gcgctaatat tgacggaaaa gaagcgttga ccaaagaaag aattcaagaa gctaaaaatt
     2941 caaatacagg attagaaaaa ttagtcaaag aaaatccaca aaattctaca agtagtcatg
     3001 gtggccgatc agtagatgtc cgagaaaata ttcaaggttc actgaatggt catgttggac
     3061 ggaatgttga tgtaagagag aatttacaaa gttcttcaaa tgctcaaggt actaagacaa
     3121 ttgatgttcg agaaaatctg caaagtagca gtacaagtca accaaaaaca gtagatgttc
     3181 gagaaaatgt acaaaattct acaagtagtc aagggtttaa atcagtggat gtccgagaga
     3241 atgtgcaagg tagcagtgca agtcaaccaa aaacagtaga tgtccgagaa aatgtacaaa
     3301 gttctacaag tagtcaagga ttcaaaacag tagatgttcg ggagaacatt caaaaagata
     3361 gtggagaaat gaaagaaaaa acacaaaaaa tcaatatcgt tgaagacaga aagtcagcga
     3421 aaaaatttga ttcaaatcat gaaacagtga ttattgatag tgaaatccgt gaaaatgata
     3481 agggatcaaa atcaagacga agattcactt atgaagacaa tgagttattc agagatacac
     3541 ttaatgatcc taatccactt ttcgataatt tattaagaaa taaaaggggg ggattgtcat
     3601 gcaattaatt ctaaagatta gtacgcatga tttcttagaa cttgacgaat tatgtgaatc
     3661 agtaaagaac gcaacaaatg aaaaaatcac ggtcactaat tatattgaac tgatgattca
     3721 agaagaatta aagatgaaaa atgaaataga gagcaatttc aaatgttgct ctctagaaaa
     3781 aaatgtgagg ggataatcaa tggatgataa acaaaaaatg tacgaaaaaa atcgcaaaca
     3841 tagtgaggcc ttaaattttc gtgtcgataa aaagacttat cttgaactga cattgattgc
     3901 aaaacaaagg ggaatttcta gagcagacgt ttgccgtgag cggttaaagg gactcaaata
     3961 tgaaccgccg ttgttagctg aagatgatag ggtagtttta accaaacagt taaatcaaat
     4021 tggtaacaat ttgaatcaag cggtaaaggc attaaatatc attgctaaat attttagaga
     4081 agaatctgaa gataaaaaag aaaagagcct acattttagt tttgatgcta gccgttacaa
     4141 catttttaaa gatgaacttg cgaatagcat agcaaaaaag ttgatgatta gttatccaga
     4201 tgacattttt ttggagtcca atcggagtgc cccctctttt tttaaattat cagaagtaga
     4261 aaaagaatat ttcgaatatt ggatagaaaa gcaaactaga ccgggtacga aagaagagta
     4321 ttcccaaaaa gttgatcgga atttaattca ctcaattcag attattgcaa gtttaaaaga
     4381 tgaggtgaaa caactttggg agctactata acatcgaaag gacgcaagaa aaatttatcc
     4441 tcgctattac aatatgcagc taatccttca aaaaaagaaa aatgtgttgc agtctcagga
     4501 ataaattgta gtgatgatag tgaggaagca ctgcaagaaa tgaatttagt acgtaccaag
     4561 atgaataaat ctaaagatat agcacaagcg tttcatgtta ttcattcgtt tgataagagt
     4621 acttctaaag agttaagtat tgataaaatg cacgaaatag ctgtcgaatt tgcagagaaa
     4681 gcttttccaa attcgcaaat cattgttgca agtcacaatg ataaagatca ttttcatagt
     4741 catcttgtca ttaataatat taacatggaa acatgtaaaa ggttgcgtat tgaccctaaa
     4801 gatttagaac taatgcgaaa aattaacgat caagtcctca atgaacatgg tcttgaatca
     4861 gtgaataatg agtactatga aaacttagaa aagagaacca atctttattc aaacgatcct
     4921 tctaagatga aatatggaaa aattaattat caagagtttg tgcaaaatgc gatcgaagca
     4981 acgttggaaa aatgcgaagt gaattctttt gaagattttt cggatcattt ggcaaaagaa
     5041 tataacattg aagtttatca attttcaaaa acaaataaca aacttggcta tgttctttac
     5101 aaacaaggtg taaatttcta tgagcgtaga ggtgagttaa ttactttagg aagagataaa
     5161 caatctagaa aagaaaaaag tcagtatgtc gaacgtactt ttagtgcaag aaagttaggg
     5221 aaaagatatt catttgaatc tgtttctgaa caacttgaaa agaatttaga agaatcacat
     5281 ataaaaaatt tttttaatat ggaaagagag caagttatgt cgctaattag tggagtaaat
     5341 tacaaaactg gtgagttaaa tgtaccgttt gtggaattag attccatcgc acaaaagcaa
     5401 tttagcgacc gtgaaggcga tgaaaaattc attaaatatg atgacttctt gcgagaggta
     5461 gacaagcatt ttgtaaatac aatgtcttta gaagatgatc aagaatacac gattaaactt
     5521 catacggatg tgaatcctca aactttacgt agtgcgaaaa gtaaaacaat ttctattaaa
     5581 aagactgatc agaaagagtt atttctaccg gcaaagggtt ttgaaaattg ttctcataca
     5641 ccttctgcta aacagattca ctatcaaatc agtcgcataa tgaatgacga tgtaaaaatg
     5701 tatcgtgcta aaaaggatta tgaattcaca caacaaatga ttgaaaatca gcaaaaaagg
     5761 caacaaaatt ttgaaatgga attctgaatg ggcttgttag tggactttcc agttttttgc
     5821 ctatgaagag ttgcttggaa agaagcagtt tttaggacat acgccagcta cccatctact
     5881 tgccagtcaa tccgccccgc tcacgctagg ctactttctg gcaagtcaga tggcgggtaa
     5941 atctctgagg gagcaagctc cctacagact ccctcccaaa aaaagaaagg tggaagaaaa
     6001 aaatgaacta tccatatttt aaagtctcgg cttcagaaga aactaaagaa atattcaaca
     6061 atttttacaa ccaaaacaaa ggaatttttg gttcaaaggc aaatatgttt cgtgttatgg
     6121 tttcaaattt accagtgtta gctagtccat caaataataa atttaatgat cctgaaagca
     6181 ttaaatttga acaaaaaatt tcagaactag aatcaatgat aagtaatgaa gtaattgaaa
     6241 agctggatga cattgatcaa aagttatcgt attctctaaa aaacaaatac aaaacggagg
     6301 agaaaaaaga tgtttaaagt ttacttcccg tttcagtggc tcgttaaagg aattctttta
     6361 gtgctactag gattgattct ttttggaagt tatctaacaa ttaaagccag acattttcca
     6421 gaagaaaagc caataaaaat tgtaacaaaa gatttaaaaa taggtgaaac ttatcaggca
     6481 gatggggttg aactaacatt taaaaatatt gaagtgaccg aaaaaagaaa tgaatttgca
     6541 caacatgatc gtgtattaca agtgacctat acacttataa atgatacaga aaatccgata
     6601 cgtccacaac aatggtcgat tgaaaataag gatggtgaag aactttatga gtatgatatg
     6661 atattggata atccagagta tattgaaagt ggcaaagaag aaacagtaaa ttactattat
     6721 gaattaccga ttgaaaggca ggactaccaa atcgtagtac gtagagagaa tggttacata
     6781 gttgggttgc catttacttt ataagtttta ttttgttagt agtttttaag gtaatatcag
     6841 ttttgtgatt tttgaaaatg gaactgggag aatggctgag gaaaataaag ttgttccgtt
     6901 tgaaacatta gtgataaata aggaagagtt tgagcaactt tttcttgatt tttttattca
     6961 atatgacatg atttatgatt ttagttcgtc actagattta tttgaattag aagcagaaat
     7021 aatagaaaac agtcgagaga agaatggtaa aatcattatt tcagtagaaa aattcaatga
     7081 attattctca caaacatttg aaatagatcc agactattat caaccacaag aagtttttcg
     7141 agagtttaga aataagatta ttggttttag tcaaactaca aaatgaaagc ttaataaagc
     7201 aagtgttgat agaattattc ctcaacgctt gctttatttt atgggcactt cataatagag
     7261 gtatagagaa gagaggtatt cattttggat actattactc caacaaactt caaagaaaat
     7321 ataggtaaag tgttgcaaga agtagttcat tcaaattctg aaatagaaat tactagggat
     7381 tctaaaaatg gattgaacga tggcgtcatt atgatttcta agagaaaatg ggagttgtta
     7441 caagaagaac tttatttaca acgaacgggt acatgggctt atgtatctga ttta
//
LOCUS       DAZKCN010000114         7395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000114 DAZKCN010000000
VERSION     DAZKCN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.117"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..773
                     /locus_tag="ACG8K3_002382"
     CDS             <1..773
                     /locus_tag="ACG8K3_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146204.1"
                     /translation="APENWYRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYL
                     LIASALAYSAVIHGSRSVLVYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINST
                     MFSTFVETFTNSTGEYAGSAESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGV
                     RGHLSDIGFFYTITQYGVGILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVL
                     LTGINIDWFYPIFTPAIPVCLAVIEYIYQECRSVANIE"
     gene            798..1907
                     /locus_tag="ACG8K3_002383"
     CDS             798..1907
                     /locus_tag="ACG8K3_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFP9146205.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            1949..3283
                     /gene="gmhB"
                     /locus_tag="ACG8K3_002384"
     CDS             1949..3283
                     /gene="gmhB"
                     /locus_tag="ACG8K3_002384"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9146206.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            3318..4955
                     /locus_tag="ACG8K3_002385"
     CDS             3318..4955
                     /locus_tag="ACG8K3_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP9146207.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            5057..5452
                     /locus_tag="ACG8K3_002386"
     CDS             5057..5452
                     /locus_tag="ACG8K3_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP9146208.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            5470..6201
                     /locus_tag="ACG8K3_002387"
     CDS             5470..6201
                     /locus_tag="ACG8K3_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9146209.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            6225..>7395
                     /locus_tag="ACG8K3_002388"
     CDS             6225..>7395
                     /locus_tag="ACG8K3_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP9146210.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGY"
BASE COUNT         2417 a          957 c         1529 g         2492 t
ORIGIN      
        1 gtgccccgga aaattggtat cgtggaggct ttttaagaat taatccacca tgttttacaa
       61 ttataataat accgattgct ttgtatatga ttgagcgaca agttgtagct agaagaaaag
      121 taaaatatta tttactgatt gctagcgcac tggcatattc tgctgttatt catggttcta
      181 gatctgtcct tgtctatcaa attattgtac ttggttgcta tatccttttt aagaaaggtt
      241 cttcgaaaag aaaaattgca atgtgggttt tagctgggtt gcttgtagtc atatttatta
      301 attcgaccat gttctcaaca tttgttgaaa ctttcacaaa cagtacaggt gaatatgcag
      361 gatctgctga atctcgattt gcttcagttt ggttttttat tcctaaattt ttgcaatcgc
      421 ctttgttcgg tactggtatt cttactggtg aggaatttac gtttatatta ccaggaggag
      481 ttagaggaca cctatctgat attggatttt tctatacaat tactcaatat ggtgtaggta
      541 tacttatatt tgatattctg tttattataa aagggttcaa aacagcatta cttgcatcaa
      601 aagtgggtat gactggctat caatatttag catttggaat tacattaaat gtcttgctaa
      661 cagggattaa tatagactgg ttttatccga tatttactcc agctattcct gtatgcttag
      721 cggttataga atacatctat caagaatgta gaagtgtggc taacatagaa taggaaatat
      781 taataggagt gcatctcatg aataagaaag agattttata taacatattg aagaagtacg
      841 aaaatttacc atatgtgttt aggaacatgt ttggtcataa tataagtaaa ctcgtatctt
      901 ggaaattgac tgctgattgt gcaacagtag aagaaaattc tgaagtttat gcagcgtttc
      961 aattgcgctc aaaggacatg tctgatgttc cagtgatggg tttagttaat tgttgcaaag
     1021 atgtagcaat tgtaatgcag gggccactat gtttaaagga tgatttctct tataatactc
     1081 tggcctacta taagaaatgt tatccaaatg ctttaattat tctttcgaca tggaaggacg
     1141 aagatattaa ggcattgaaa aggatagagg atcttggagt tatagttatt tgtagtgaga
     1201 aacctgaaaa gcctggacac ttaaatatta attatcaagt tggaaacact ttgcctggaa
     1261 tagtcagagc aaagcagctt ggtgcaaagt atgtttgtaa aactagaaca gatcaaagaa
     1321 tataccatcc aaatgcaatg gcgtttttct gcagtttact tgaacagtat cctgttaata
     1381 acgaagatga ttggaaatta atacaaaatc agcgattact gttactatca atgccgtatg
     1441 gagacatgtt tttcccgtat tgtttgtcag acttcctgta ttttggtgat gttgatgata
     1501 tgataaacct cttctcgatt cctagtgatg tgagagaaaa gggagctgtg tctagaggag
     1561 tctcaagaag ggagatatct gaaaataatt tagctccaga agttcagctt cttcgaagct
     1621 atatttcacg aatggggggc aatgaggagt gctctattag ggcatattgg gagtttgtaa
     1681 aaaaccatgt tattactatt aacaaaaatc aaattgattt gtactggcat aaatatgtag
     1741 gtcgttattc aaataatact atttatggta cgtattatat tgatgatagc caagatgcac
     1801 ttcactgcta taattttgat tttataaatt ggttgaattt gtacactgga aaatacgaat
     1861 acaaagcaga atatgaaaaa tatatggact ttgtttggga agagtaatta acttttatag
     1921 taaaaagtac tttagggaag ggcatattat gaaaacagta attatggcgg gcggacgagg
     1981 aagccgaatt tcggaattgt ttccggatat tcctaagcca atgattccta tagatggcat
     2041 tccagtttta gaacgagaat tgtgttcgct tagagatcaa ggctttaatg atattatttt
     2101 gactgtttcc tatttgcatg aaaaaataga ggagcatttc ggagatggtt caaaattagg
     2161 aattaaaatc aaatatttta ttgaaaaaac tccacttggc aatgcaggtg cattgtattt
     2221 tttgttggat gaactgggtg atgaaccgtt tttacttttg aatgctgatg cagtatttga
     2281 tgttgatttc aggcagatga ttagatttca tgttgagaat aacgcagatg ttactttgtt
     2341 tacgcatcct aactcacatc cgtatgatag tggattaatt atatccgatg acaataagaa
     2401 agtgcttcaa tggttagcga aggaagatca aagacctgaa tattataaaa accgagtaaa
     2461 tgcaggtctt catgttataa atccgaaggt gttgagagaa aatccattaa tggaaattgt
     2521 aaataaatca atagttgaag gtggggggct accatacaaa attgatttag atagaatgat
     2581 tctgaaacca ttggctaaca cagggcgtat gtattgctac gattctccag agtatgttaa
     2641 agatatggga acaccggacc gatatagagc tgtaattaag gactttcaaa agggaattat
     2701 tgttggtaaa aatctatcca atccacaaaa agcaatcttt ttagatagag atggaacgat
     2761 taataaatac gttggttttt tgagaaatgc agaagaattt gagttaattg atggagtatc
     2821 ggaggcaatt aaaattatta attctctagg ttttctgtgt attgttataa caaatcagcc
     2881 tgtaattgca agaggtgaag ttactgttga tgagcttaat agtattcaca ataagcttga
     2941 aacaatgcta gggcatgctg gtggattttt aaacggtatt tattactgtc cgcaccatcc
     3001 ccataaagga tatgcaggag aggttataga attaaaaatt gattgcaatt gtcgcaagcc
     3061 aaaacctgga ctgttattag aagcatcaaa ggattttaac ataaacctaa gtcaatcatt
     3121 tatggttgga gatagtgaaa gtgatgtaat tgctggaaaa aatgctggtt gtaagacagt
     3181 actgattaca gaagacatca ccttgcctgc taattatggc caagactata gtgtctgttc
     3241 attgttagaa tttgttaaaa atcatttatg cactatgcaa taattggttt agtgaaactt
     3301 gtaaaaggag taattagatg aaggtattaa ttccagcagc aaaagttgtt gaaaaagaat
     3361 tgcagagtat aggaatgtta ccagcaatta tctatccaat taataaaaaa attgtctttg
     3421 attatttgta taatcagtat aatgcaatct gcgattcatt ggatattatc tgttatgaaa
     3481 aagcggataa ggttcataga agactgaaaa actatcatga ttccagaatt catatatgtg
     3541 accttcctga attaggggat ctagggcaaa ccgtgtattt tgggttatca gatgaagatg
     3601 agcctgttct aattaacttc gcagatacaa ttgttatgga gagtatgtct gatatagaaa
     3661 tggatacatt ctattattct gaagattttg tgtcgagtac ttggacatac tttaaaatta
     3721 atgaaggaat aattactgat atccacgata aagaggaagc ttacgtatcc gataaacgtg
     3781 aaaaattatt tattggcgta tttatgtttt cttcgtcaaa acttctgaag agttgcttaa
     3841 gagaggcagt tgagagtcgg aaacaggggg gaataaactc attttatcgg gctttacagc
     3901 tatacagcga acgttgtccg ctaaaggccg taagaacaga aaagtggttt gatattggac
     3961 acatcgataa atattacaat acaaagttgg aagttaaagc acgagagttt aatcatatct
     4021 ctatagataa agatcgaggg atattgaaga aaactagcga tgacaaggat aaatttattg
     4081 gagaaataaa gtggtattta aaacttcctg ctgatattga atatgtaaga cctagaattt
     4141 ttgattattc tacatcttac gtagctccct atgttgatat ggaatattat gcatatcata
     4201 cggttcatga gttatactta tatggtgatt taaaatataa ccagtggcat gatctgttta
     4261 gccgtataaa gtttatctgt agcgatttta agcgatacac cttaaaagat gaaaagatta
     4321 gagagtcatt ggaagatatg tatttgcgaa aaaccatgca gagatttcaa aaattgtctg
     4381 aagataaaag attttcagct ttttttgaga aacaaattca aattaatggt cgtaagttta
     4441 tttctttaac cggtgtaatt gaacttctaa agaatcttat cccaagtatg ctctatgatg
     4501 taaatgaatt ttgtattata catggagatt tgtgttttgc taatatgatg gtagattcca
     4561 attgttcctt tgttaaggtt attgatccta ggggaaaatt tggaaaatat gatatttatg
     4621 gggacagcag atatgaatta gcaaaactat ttcatagttt agatggtaaa tatgattata
     4681 tcatcaagga tctatttact gtagaatatg ataccgagtg tgcagtgata gattatacaa
     4741 ttaatacgcg tgtaagagat tacgatttat ttgaattgtt taagtcggtc ttttcggacg
     4801 agattaagga cgattttgat aagattgtat tgatagaagc gttgctattt cttagtatga
     4861 ttccattaca cggtgaaagt tttgatcacc aaatagtgat gcttgctaca ggtatggaac
     4921 ttcttgcaag agtaagtaat aaagttatac tctaatataa attgtgcctt aatgaataac
     4981 ggttacaata ctgttttagc aatgtaggca ttaaggcacg attatattaa ttaatacaat
     5041 agaagggaag agaaaaatgt atagtgaata ttcttttgtg tttgatatcg atggcacttt
     5101 gtgtccgata aagaagaagg atgagcgata tgaggatttg gtgccgtatg agaatatggt
     5161 tgaaaaactg agattctata aggagaatgg tgctaaaatt acattgttca cctcacgcaa
     5221 tgttaattcc tataaaggaa atttgggaat gataaatgct aatactgcaa aagtgctctt
     5281 ggcgtggttg gataaatggg acattccgta tgatgaaatc ctgtatggta agccttggcc
     5341 ggggcataaa ggcttctatg tagatgatcg aacggttaga cctgatgagt ttttgagatg
     5401 ctctgtagat gaattaaatg aaatctgcaa cagttcaaaa aaaagattct aaataaggag
     5461 gttagttcta tggaaaaaaa gatgacagtt atcattacga tggcaggttt gggaagtcgt
     5521 tttcgaaaaa ttggttatgc ggttcctaaa tatatgatta atgccaaagg aagaacttta
     5581 tttgattggg ccatggatag tttggttgga tacaataaaa atgttgataa gtatattttt
     5641 gttgttcgtc aggaagacaa cgcttcagct tttataaagg agcattgtgc ttactttggt
     5701 attgaaaacg tagaagtgat tgaaatagac tatatgacag atggtcaagc aactacatgt
     5761 atgttggcta ttccttattg caatcaagac aatgagatta tggtttacaa tattgataca
     5821 tacgtggaat caaatgagat gaaatacgaa gatatttgtg gagatggaca tattccatgc
     5881 tttcatgctg atggcgatca ttggagtttt gcaaagctta acgatcgaaa tgaagtaata
     5941 gaagtccgcg aaaaagttcg catttctgat aattgtactc ttggtgcgta ttatttctca
     6001 tcggcaaagc tgtataaagc tttatatgaa gagtattatg cagactcctc taatatggag
     6061 aagaatgaaa aatatatagc accattatat aactttatga taaaaaaagg gttaaaggtg
     6121 actattagca tagtagatgc gaaaaaggtt catgtgcttg gtactccaga agagctcaat
     6181 gtgtttttga atcaagaata aatagtgttt aggagaaaat agcagtgacc agtaataatc
     6241 taattgtaaa aacattaagc agatataaga atactccact tcctgttaag gctgcattat
     6301 ggtttatggt atgtggtttt ttacagagag gggtttcaat gattacaacc ccaattttta
     6361 ctagagtttt gtctacagaa caatacggtt tatacagcac ttatctatcg tgggaatcct
     6421 tattgctcat gattacatcg cttagtttgt tcaaagccat gatgaatctt tatgtaaaat
     6481 atgataatca agaacatatt ctttcgacag tatccggatt gactttagta ctgactattt
     6541 tttggtttgt tattgctatt tttattaagg atatattggc agaaatttta ggattgacac
     6601 cagttctagt tgtctgtatg ttctcatatt ttatatttca ggcagaaata caatgttggt
     6661 ctttacacaa acgatatata tatgattata agactttatt agtagctacc cttgcagtta
     6721 ctgttggctc atctgtagtt ggctgtttag cggttctgtt tatatctcct accgctgagg
     6781 cgagagcctt aatgaatgtc ctagtgactg gtgttgttgg cgtggctgtg tatatatcgg
     6841 tatttagaag atgtaaggat tttaaggata atgaagtgtg gaatttcgca gtagtatttt
     6901 gtgtatcgtt gatgccacac tatttatctg aatttattct gaatggttct gataaaatta
     6961 tgataaatta cttatgtggt tcgtcagatg ttgctattta cagcgtggca tattctattg
     7021 gtgctctaat taaccttgtt acaaattctg tcaattccac ttttgcacct tatcagtatc
     7081 aaaaaatcaa gtcaggagaa tatgatttac tatcaaaaag agctaatgaa gttcttggtt
     7141 tggtagcggc tatgcttttt gcattaatgc tatttagtaa agaaattgtg ttgatatttg
     7201 gtgggcagaa atatatcgac agtgttaatt taattgtacc tatatgtatc ggtatttatt
     7261 tcaactatat gtttcagctg tttgctagag tacaggaata ctttgaaaag aaactcactg
     7321 tcgttattcc atcggttttg tgtgcaatta tgaatattgt actaaattat gtttttataa
     7381 agttatatgg gtatc
//
LOCUS       DAZKCN010000115         7254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000115 DAZKCN010000000
VERSION     DAZKCN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.118"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8K3_002389"
     CDS             99..746
                     /locus_tag="ACG8K3_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP9146211.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8K3_002390"
     CDS             1139..3784
                     /locus_tag="ACG8K3_002390"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9146212.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8K3_002391"
     CDS             4094..5407
                     /locus_tag="ACG8K3_002391"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP9146213.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8K3_002392"
     CDS             5397..6050
                     /locus_tag="ACG8K3_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9146214.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8K3_002393"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8K3_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9146215.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8K3_002394"
     CDS             6947..7147
                     /locus_tag="ACG8K3_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9146216.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2396 a         1229 c         1593 g         2036 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt attt
//
LOCUS       DAZKCN010000116         7159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000116 DAZKCN010000000
VERSION     DAZKCN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.119"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1316
                     /locus_tag="ACG8K3_002395"
                     /pseudo
     CDS             24..1316
                     /locus_tag="ACG8K3_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1835..2569
                     /locus_tag="ACG8K3_002396"
     CDS             1835..2569
                     /locus_tag="ACG8K3_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9146217.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            2585..3754
                     /locus_tag="ACG8K3_002397"
     CDS             2585..3754
                     /locus_tag="ACG8K3_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9146218.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            3770..4765
                     /gene="lacD"
                     /locus_tag="ACG8K3_002398"
     CDS             3770..4765
                     /gene="lacD"
                     /locus_tag="ACG8K3_002398"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP9146219.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            4844..5770
                     /locus_tag="ACG8K3_002399"
     CDS             4844..5770
                     /locus_tag="ACG8K3_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP9146220.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            5789..7039
                     /locus_tag="ACG8K3_002400"
     CDS             5789..7039
                     /locus_tag="ACG8K3_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9146221.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDANRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2349 a         1194 c         1470 g         2146 t
ORIGIN      
        1 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gccgaaaagt
       61 tagctgaatc cattctgaat gcctatcaac ctgaatctgt cgatgacatg caagatgctt
      121 tgaaagatgt gtttgggccc ctttttgaaa aaatgcttca aggagaattg aataatcatt
      181 taggttatga tgcccattct aaagagccta aggaacacga taaccgtcga aatggctatg
      241 gaactaaaac gcttaaaacc agttttggtg aagtagctat tgatgttcct agagaccggg
      301 aagcttcctt tgaaccagag ttaattccta agagaaaacg agatgtctcc gacatcgaag
      361 ggaaggttct ttccatgtat gcacgaggaa tgagtcaatg agatattgcc gcaaccgtcg
      421 aagctattta tggctttgat atttcccatg aaatgatttc agatatcact gacgctgtcc
      481 ttcctgaatt ggaagaatgg caagcccgcc ccctagcaaa gtgttatgcc tttctattcg
      541 ttgattgtat gtatgttact ttacgagaaa attatgaagc caaagaatat gctgtacaca
      601 ccattcttgg ctatgatctc aaaggaaata aggagatttt aggattatgg ttaaatcaaa
      661 cagaatctaa aaatcgctgg atgcaagtat ttgatgaact gaaagcacgt ggtgtcgaag
      721 atgtcttttt catttcgatg gacggtgttt ctggcctcga agaaggcgca aaagcgatct
      781 ttccatcggt aattgttcag cgctgtattg ttcatcttgt tcgaaatgca ttgcgctata
      841 ttccaagtaa ggactataag gaagtctgcc gagatatgaa aaagttctac ggtgcttctt
      901 ctctaaacgc tgcacatgct gcttttgaca gttttcaaaa tcggtggtct cattattctg
      961 gtgctgtaga tgtctggaaa cggaactttg cacatgttga acaactattt gattatggta
     1021 gtgccattcg aaaaattatg tacactacca atgcggttga aagtgtccac tccagcttca
     1081 gaaaagtcac aaaaaaagga gcgttctcta atgagaatgc actcctaaaa ctactttatt
     1141 tacgcacgaa agaattacac gcgaagtggt caggtggtcg aattcaaaac tgggctatgg
     1201 tgcttaatca gttaatgatc aacgagacct tctcctctag aatgaaaagt atgcgattta
     1261 ccttccgtaa caacggtagg tttattcaac tatgtgtccg atcaaaattt atctgatttc
     1321 aaaatagccg agagaattat ggtggttaaa aactcttaac tatttaactt ccacagttta
     1381 gttgacaaac ccacttaatt ctgataacga aaaacatatt cgaatgatta aacagaagta
     1441 aatacttatt taaataactt ttaattttca tgttcataaa tgatttgaaa ccaaagcaaa
     1501 tgcgttgcac ttttcctccg tcttccgaaa atcgttcaat tcactggtaa gtagtcattt
     1561 gatagtttga ggcaaattgt tgattgatga agggtaggag ctaaaaaaag ataccagttg
     1621 aagagagaaa aagaaaaaaa tagagggtgt tcttgaatga aaaaagttgg aaaattattt
     1681 ttcatttcct tttattccta ttaaaatcag tgatgtcaat catattaaca agactaaaaa
     1741 tgaaaataaa aaaaagattg ttcttagtat tgactcataa aaaataccac tactatactt
     1801 acattgtggt tatagccagt taggagagac atcgatgcaa aatacctttc aatcaaaatc
     1861 actttattat cagctggtag aaatattgaa acaacgaatc gaaaacagta tggtcgctca
     1921 tgacaagctg ccatcagaaa gagagttgac cagtttttat ggcgttagtc gcacgacggt
     1981 gcgactagca ttgcaagaac tggaaaaaat gggctacatt tatcgacagc atggaaaagg
     2041 aacctttgtt tcggaattaa ggacaaatgc gactgatcta gctggcgcat atagttttac
     2101 cgaacaaatg cgggcaatgg gcaaacatcc tgaaaccaag atattggcct tcaagaagat
     2161 tgaagcaact aaatatttag cgagtcatct gaatgttcaa ctaggtgagc ctttatttca
     2221 attagaccga ttgcgcttag ctgatgattt gccaatgatg atcgagaaat cctatttgcc
     2281 agtgaagcta tttatgtccc tttctcaaga aattctattt ctgaaacctt tatataattt
     2341 gttttcagag aatttcaatc aaatcgtacg gttggctgac gaggaatttt atgccagcat
     2401 tgtccagaaa gaagatagtg agttgctaaa tattccagaa ggatcaccag tgctccattt
     2461 agttcgctca acctataatc tcgagaacga agtgattgaa tttacattaa gtgtagcgcg
     2521 ggctgataaa tttcattata agatcaggca tctgcgttcc actaactgaa ttggaggaaa
     2581 gaaaatgttt atgttctcaa aagaagaact acgaaagcta ggagcagaaa atactacccg
     2641 agaaattcga caacaaccgg aactgtggca tgagacgttt gaccgttatc aagaaacagt
     2701 tgcagattta gatcgtttgt ttacggatgc gaaacaacat gctggagagc gaccaatccg
     2761 agtcattttt acaggggccg gaacctcgca atatgtggga gatacactaa ttccgtttct
     2821 tactagtcaa ggaaatacga accagtttat ttttcaaagt attggttcga ccgatatcgt
     2881 tgctacgcca aaagaatacc tgattcctga tgagacaacg atcttggtct catttgcccg
     2941 tagtggaaat agtccagaaa gtctagccgc agttaagatg gccaatcagc tggtcaacaa
     3001 gatctatcat atcgtgatca cctgtgcacc agacggatta ttggctacac aaatgcctac
     3061 aaaaaaagaa aatctagttt tactaatgcc aaacaaagcc aatgacgctg gttttgctat
     3121 gacaagcagt ttcagctgta tgacgctgac tgcgctactt atttttgatc aagcaagcaa
     3181 tcagcaaaaa gaaagatatg ttcatcaatt aagccgacta ggagaagaag tcatccaacg
     3241 agaatcagag atccaagggt atctggcatc agactttcat cgcatcattt acttaggttc
     3301 gggaagttta ggcggattga ctagagaagc gcaattaaaa gttctcgagt taactgctgg
     3361 aaaaatcgca accgtatttg atacatcttt aggattcaga catggtccca aatcttttgt
     3421 caatgagcaa acattggttt tcctgtttgt cagcaatgac tcatatacgc agaaatacga
     3481 tgtagacatg ctggaagaaa tcaaaaatga cggtattgcc caacgcgttg ttggaattgg
     3541 tcaaaagggt tcattgttta gtggtgattc ctttgaatta gttgcagatt cgttactgcc
     3601 ggatggctat cttgctttag cagatatcat ggtagcccaa acgatcgcat tactttgttc
     3661 ggtgaaggtt ggtaatacac cagatacacc atcgccaagt ggaacagtca atcgtgtggt
     3721 caaaggcgta acgatttatc catatgaaaa ataggaaaga gtgtgaagca tgttagaatg
     3781 gtcggaaaat aaaagacaag caatggaacg tctatcgacg ccagaaggca ttattaccgc
     3841 tttggcgatc gatcagcgag gcgcgctgaa gaaaatgatg tcggctttag ggatggaagc
     3901 gacaaaggaa aaaattgagc agtttaaaac actaatttcc caagaattga ctccttatgc
     3961 ctcagcaatt ttattggatc ctgaatacgg actgcctgct gcacagaagc gtgcagcagc
     4021 gagcggtctg ctgttggcat atgaaaaaac tggttatgat caaaccgtac ccggccgctt
     4081 gccggatctg ctgtcggact gttcagttct gcgattgaaa gagttaggtg cggatgcggt
     4141 gaaattttta ctgtattacg attatgaaga agcagaggaa atcaatcggc aaaagcaagt
     4201 ttttgttgaa cgaattggaa gcgaatgcat ggctgaagga ctgccatttt atttagagtt
     4261 attgtcatat gatgctaaaa atgtagatac gaaatcgctt tcctatgcca aagcaaaacc
     4321 gcgcaaagtg atcgaaatga tgaaagaatt ctcgaaaacc cagtataaag tagatgtctt
     4381 aaaggttgaa gtaccagtaa acatgtcatt tgttgaagga aatgcaacag gggagatcgc
     4441 ctatacacgt caagaagctg ccgcttattt taaagaacaa agcgatgcaa cagatttgcc
     4501 gtatatcttc ttaagtgctg gggtatcagc ggcattattc atggagacat tgcgttttgc
     4561 caaacatgct ggggcacgat tcaacggtgt actctgtggc agagcaactt ggaaagatgc
     4621 agtcgctcct tttgttgaaa aaggagaagc ggcaacttta gaatggctga cagaacaagg
     4681 gacacaaaac attcgtcaat tgaatgaagt gataagagaa actgccatcc catggtatga
     4741 gaaagtaagt gaaagcactt gttaatgttt ctaatatatt agtagtgata agagatatgt
     4801 gtgattataa tttatcatta ttgaaactgg gaggatattt tatatgaata aaagattgtt
     4861 agattattca gcttcagatt ttttgaaagc taagccgatg gaattaaagc aggcaatttt
     4921 ggcttctgaa ggtagaacaa ttatggcaga aaatgtgcca agtagctgtc cttacatggg
     4981 aaaatcagtg acaaatgcag aattggagag agctgctgga gctgatttaa ttttgtttaa
     5041 tgcactagat ctttttaatc ctcaaatttc aggtgtacct ttaactctaa aagagaatcc
     5101 aataatctgg attaaaaaag ctttaggaag agctattggt gtaaatttag aacctgtaga
     5161 caaagaagtt ttgatgagtg aaaatcgaac tgaaatttca agtggtagaa tcgtttctcc
     5221 tgagacttta aaaaaggcaa atgaattagg ctttaatttt atttgtttaa ctggaaatcc
     5281 aggaacagga gtatctaata gaactattga gaaagcaata acattatcca aatcaatttt
     5341 taatggatta attatcgctg gaaaaatgca tggtgctggt gttgatgaac caattatgaa
     5401 cttagatata gcaaaaagat ttattaaaac aggtgtagat attttattag ttcctgctcc
     5461 atacactgtt ccgtatttca tggaaagtga tttgcgagga atcgttgatt atataaaaga
     5521 ttttaataga aataaatcta ttgaggaaaa agttctagta ctaacggcta atggaacaag
     5581 ccaagactct tctgaccaag aaactattaa aaaaattgca ttagcatcaa aagcatgtgg
     5641 agctgatatt caacacatag gggattcttt aaatggtatt tgtcttccag aaaatatttt
     5701 tgctttgggt caagcaattc ggggatatcg tcatcaaatt attatgttag gaaaatctaa
     5761 ttatcgttaa ttaaggagga aatgaaagat gatcgacaaa gttcaaaaaa tagctgcaaa
     5821 aattagtaat caaagacatc tactagcttt aagagacagc atggctgcag ttatgccatt
     5881 aatcattatt ggttcctttt ttatgttatt agcaaatttc ccgatagaag gatttacaaa
     5941 atggttatct gaaattggag tacaatcctt tttgaacaaa gcatcagatt caacatttgg
     6001 tattattgga ttagctgtta cctactcttt gggacacaat ctagcaaaga cttatggtgt
     6061 agactcatta tcagcaggat tattatcttt gagtggatat gttttactta cacctttatt
     6121 ggatgcagat ggttctatag gatttccaac aaaatacttt ggtacctcgg gtctttttgt
     6181 tggtattatt gtagcacttg tatctacaga aattttccgg tggtttgtac ttaaaaatat
     6241 tacaattaaa atgccggcaa gcgttccacc aaatgtaagc cgagctttcg ttgctattat
     6301 tcctggcttt tttgtcgttc tactatggtt tattgttgtt gtaatttgtt ataaacttgg
     6361 aattgaaaat gttcatgctc ttattgcaga tacactcgca aaaccactat ctttactgac
     6421 gaaaacttta ccaggaatta tattggttat acttattcaa tgtttctttt ggatgtttgg
     6481 tattcatggt gcacaggtaa cggggccaat aattgaaccg ttattattgc aaaactctga
     6541 tgcaaataga attgcttatc aagctggaaa agaacttcct aacattataa cttatgaatt
     6601 cttatataac tttgtttttt ctggtggtgc aggttgttta tttgcacttg ccattttaac
     6661 atttttcttt tcaaagagtc aggaaaataa aactttaggt aagttatcaa tcgctccagt
     6721 aagttttcaa gtagcagagc cccttctatt tgggtttcca actatattaa atataaaaat
     6781 gttaattcca tttgtaactg caccagtagt aacaacatta attacttatt tttcaatgag
     6841 tatgggatta gttgcaaaac ctgtaggagc aacaatccca tggacaactc caccgataat
     6901 tgccggattt ttagcttctg gtggacgaat aagtggtgca gtgattcaag taataacaat
     6961 tgcaataaat gttttgatat actatccatt ctttaaatta gatgataatg ccaagctaaa
     7021 aagcgaaaaa aatgattaga actacctata tgtttctaac tgtagttaag tatgaaaaga
     7081 ctatttttaa atttagcatt tttgcgctat cggcgtagat gaagtatcac gataacgaaa
     7141 ttagtttctt tcatttaga
//
LOCUS       DAZKCN010000117         6921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000117 DAZKCN010000000
VERSION     DAZKCN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.120"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1074
                     /locus_tag="ACG8K3_002401"
     CDS             202..1074
                     /locus_tag="ACG8K3_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9146222.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1071..3029
                     /gene="nagE"
                     /locus_tag="ACG8K3_002402"
     CDS             1071..3029
                     /gene="nagE"
                     /locus_tag="ACG8K3_002402"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP9146223.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3280..4131)
                     /locus_tag="ACG8K3_002403"
     CDS             complement(3280..4131)
                     /locus_tag="ACG8K3_002403"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9146224.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4254..4721
                     /locus_tag="ACG8K3_002404"
     CDS             4254..4721
                     /locus_tag="ACG8K3_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP9146225.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(4984..5466)
                     /locus_tag="ACG8K3_002405"
     CDS             complement(4984..5466)
                     /locus_tag="ACG8K3_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9146226.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5566..6429)
                     /locus_tag="ACG8K3_002406"
     CDS             complement(5566..6429)
                     /locus_tag="ACG8K3_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9146227.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2080 a         1424 c         1249 g         2168 t
ORIGIN      
        1 aaaagaattg acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt
       61 tcatgtgact agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag
      121 gtttttcgtg tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa
      181 atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg
      241 gtagatgacg gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa
      301 agaaatgatt tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt
      361 caaatcaagt tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa
      421 acgatcattg atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc
      481 gctttgaccg accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc
      541 aacaaacttt taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg
      601 gcagtagagt atctgacaaa agagcttggg attccatata cttatgacga agcaggatat
      661 atcgcaatcc atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt
      721 gaagtgacga ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc
      781 cattcagaac cattttcact gaattattca agactggtga atcatttacg actgcttcta
      841 caacgaaccc atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa
      901 cgaaaatacc cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa
      961 taccagctcg cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta
     1021 agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat
     1081 atctacaacg aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt
     1141 tactagtagg gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt
     1201 tacaagctgg cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag
     1261 cactagggat gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg
     1321 aagttccagt aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag
     1381 aatcagttga tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat
     1441 tgattgcggc agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt
     1501 tctttagcgg caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg
     1561 cagtgctact gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca
     1621 ttttgagttt aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc
     1681 ctacaggatt gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg
     1741 atattggtaa atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg
     1801 gatttttccc aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg
     1861 cacgtccaga aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat
     1921 tctttactgg tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat
     1981 atgtcttgca tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga
     2041 cagcaggatt tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga
     2101 ttgcgaatca gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact
     2161 ttggctttaa ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag
     2221 atctttcaga tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa
     2281 gcaaaatcta cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta
     2341 cacgccttcg tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca
     2401 ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag
     2461 aagtacaatt cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg
     2521 cacaaacaaa caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata
     2581 tttacgctat cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg
     2641 cggaaaaaat gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc
     2701 caatcgctgg tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag
     2761 atgctggtat cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac
     2821 cattcacttt atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag
     2881 ttgatcttgc cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa
     2941 atggcgacaa agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata
     3001 aaatcggctc tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat
     3061 aaacaaacag gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc
     3121 gttctcctgc caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt
     3181 tttatttgac tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat
     3241 actcaaaact aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc
     3301 ttttaatgct gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga
     3361 ggaaatagct aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc
     3421 ttcttgaggg aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat
     3481 aatttctaac tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt
     3541 tagattgccg gataattcta agatttctgg agagtcactt tctactagta cttttgctgc
     3601 agcttctgca tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac
     3661 gaattgacca gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt
     3721 attccgtaaa aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg
     3781 atctggcgct aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata
     3841 agatacccca gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc
     3901 tggaacgcca gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc
     3961 taaatcatca taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt
     4021 ttcttcacta cgtactagta catagatttc attttgtgga accatctttt ttaagaattc
     4081 cagcgcataa ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac
     4141 tccctattct ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc
     4201 ataaagagtg tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt
     4261 attctctgca atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata
     4321 cccgatcact ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac
     4381 gtaaaatcat gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc
     4441 agccttccct tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg
     4501 aaggtaatac acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg
     4561 cgaatatcca agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag
     4621 aaatggctaa gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa
     4681 aaagaccaga aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc
     4741 atttttctga tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg
     4801 tcatgcccct ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat
     4861 aaacggtgtt caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag
     4921 ctatttccca tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata
     4981 ttatcatggt ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac
     5041 tcccatttcg actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa
     5101 aacatgccca aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa
     5161 agcgcttaca acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac
     5221 atcctgactc gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac
     5281 tgtaaagaaa taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat
     5341 aactttttgc tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact
     5401 cttcaattcc acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt
     5461 tgccatttct cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa
     5521 tgacacgatt acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt
     5581 cactgcaatc caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga
     5641 gatctcggga gcttcgacta acccataatc tgaagaagga agccattctg catagatttt
     5701 cccccaagta tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt
     5761 gtgttcttct atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa
     5821 gccaatcatg tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga
     5881 aacgttgatt acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt
     5941 ctccgtgatc gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg
     6001 aacttttttc gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat
     6061 cgattttcct cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc
     6121 gtatctactg atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc
     6181 ttcgactgtt tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag
     6241 atcgcgatta gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc
     6301 aataaacgta aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat
     6361 tttataggaa agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc
     6421 tttaagcata tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa
     6481 ttacttgatg aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag
     6541 aaaattctca agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta
     6601 cctttagaaa aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat
     6661 cttttattcc cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa
     6721 ctgctgatca gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat
     6781 tggaacaatt atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa
     6841 aaaaagttcc atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa
     6901 gaaaaggaga atgaattttt g
//
LOCUS       DAZKCN010000118         6892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000118 DAZKCN010000000
VERSION     DAZKCN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.121"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8K3_002407"
     CDS             <1..227
                     /locus_tag="ACG8K3_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146228.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8K3_002408"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8K3_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8K3_002409"
     CDS             1635..1841
                     /locus_tag="ACG8K3_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146229.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8K3_002410"
     CDS             1862..2458
                     /locus_tag="ACG8K3_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFP9146230.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="ACG8K3_002411"
     CDS             3410..4009
                     /locus_tag="ACG8K3_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9146231.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="ACG8K3_002412"
     CDS             4058..4774
                     /locus_tag="ACG8K3_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9146232.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="ACG8K3_002413"
     CDS             4788..5567
                     /locus_tag="ACG8K3_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9146233.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="ACG8K3_002414"
     CDS             5650..6006
                     /locus_tag="ACG8K3_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9146234.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="ACG8K3_002415"
     CDS             5996..6262
                     /locus_tag="ACG8K3_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9146235.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="ACG8K3_002416"
     CDS             6421..6699
                     /locus_tag="ACG8K3_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146236.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6892)
                     /locus_tag="ACG8K3_002417"
     CDS             complement(6696..>6892)
                     /locus_tag="ACG8K3_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9146237.1"
                     /translation="NNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKE
                     GTLFGFSVCTEIRILLGIPA"
BASE COUNT         2534 a          959 c         1335 g         2064 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatga tcttgttcta ttaaattgtt aa
//
LOCUS       DAZKCN010000119         6754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000119 DAZKCN010000000
VERSION     DAZKCN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.122"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8K3_002418"
     CDS             <1..888
                     /locus_tag="ACG8K3_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP9146238.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(1373..2002)
                     /gene="udk"
                     /locus_tag="ACG8K3_002419"
     CDS             complement(1373..2002)
                     /gene="udk"
                     /locus_tag="ACG8K3_002419"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9146239.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(2086..2781)
                     /locus_tag="ACG8K3_002420"
     CDS             complement(2086..2781)
                     /locus_tag="ACG8K3_002420"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP9146240.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(2794..3510)
                     /locus_tag="ACG8K3_002421"
     CDS             complement(2794..3510)
                     /locus_tag="ACG8K3_002421"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP9146241.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            3840..4445
                     /locus_tag="ACG8K3_002422"
     CDS             3840..4445
                     /locus_tag="ACG8K3_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP9146242.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            4770..5093
                     /locus_tag="ACG8K3_002423"
     CDS             4770..5093
                     /locus_tag="ACG8K3_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP9146243.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            5275..6645
                     /locus_tag="ACG8K3_002424"
     CDS             5275..6645
                     /locus_tag="ACG8K3_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP9146244.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT         2053 a         1271 c         1243 g         2187 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc attagggggt gcgacaaaag agcttcttca aaaaaataag tcgaaatctc
      961 acacaaattc gagaaataat tttcgtaagt ttcttcttat ttattgcatc tgaccacttc
     1021 tgttacaacc tctcgttaaa cggtgttcaa aagcttgcat ctacgataat aagcccaacc
     1081 caacgaaaaa cgtgaggagc tgttttattt ggatgctctt atcgcttgat gcaaagacag
     1141 cttttttaca acctctactt cacggtgctc aatcagctga ccttcggtat tagctcaaaa
     1201 aacggtaaaa tataaacagc tattttccgt ttcttctttt taatacttag gtcaagatgc
     1261 tgttttcaca acctcttgtt ataaataaaa aaacagttgg ctggtgactg ttcaaaaagc
     1321 agaatccttg cttttcaaac ggtcgcagtc cgttcaactg tttcgctgcg cactatttat
     1381 ggtttaagaa actagctact tttgttgtaa tcaaatcaat agcaacgtgg ttttctccgc
     1441 cttctggaac aatgatatcc gcatagcgct tcgttggttc gataaattga tgatacatcg
     1501 gtttaacaac tgtcaaatat tgttcgatca cggagtccaa tgtgcgtccc cgttcttcca
     1561 tatcccgttt gatacgacga atgatacgaa tgtcatcatc tgtatctacg tatactttga
     1621 tatccatcaa gtcacgcaga cgttcgtctt ctaaaatcag gattccttct agaatgatca
     1681 cttcttttgg ttcctgaatg atcgttgcct gacttcttgt atgcgctaca tagtcataca
     1741 cgggtttttc aatggcttta tagttcagta agtcgcctac atgctgaatc agcagatcat
     1801 tatcaaaggc aaatgggtga tcgtaatttg tgttcaatcg ttcttcaaaa ctcaaatggc
     1861 tctgatcctt gtaataagaa tcttgttcaa gcatcatgat ggaatggtcc gggaagttgt
     1921 tgaaaatcgc acggctgaca cttgtctttc cgcttccgga tccacccgtc acaccaatga
     1981 taattggctt gctttttgtc attgactaaa acctctttcg tatccgtttt ttcattctaa
     2041 tctattatag cgaaaaaaca cgaaaatgct atatttttct gaaaattaat ccttttcaat
     2101 ttccaataat gaataaagtt cctctaatga atcgattcga taagtaggct ggatttcttc
     2161 gatttctggt aatttatttc tattgaacca aatcgtatcg atccctgcca tatttcctcc
     2221 tttgatatca gaagtcaaag aatcaccaat gatcacagtt tgctttttat caaaattagg
     2281 aatgtgggaa aagacatgat caaaaaattc tttcatcggc ttttgcgctc cgacagcttc
     2341 tgagacaaag atatccttga agtaatcaag cattcctgca tctgttaatc gccgatgttg
     2401 cgtgtgcgac acaccgttcg tcacaatata aagatcagca tgcggatgga cctttttcag
     2461 gatttctaga cttcctggaa caagatcatg accttggttc aagtagtggc ggtagcgttt
     2521 ttccatttga tcactatcta ctgttttccc aaattgctgg aaaagtaacc caaaacgact
     2581 gcctattatt tcttctcgtg atagtctgcc tttttcaaac tcctgccata aatgatgatt
     2641 caaagcctta tagctggttt cgatttctgg tgtcaatgtg attccttgtt ctgcaaacaa
     2701 agcacttaaa gcattggctt cagttaattg aaaatctaac aatgtatcat ctacatcgaa
     2761 caataaagtt ttatatttca tctttttgac ctcctattta aaataaatac ttaacttttt
     2821 catctctgct aatgtcaacg atcgatactc gcctactcct agctgttcat ctaattccaa
     2881 tggtcccatg cttagccgtt tcaatgcggt cactttcttt ccttgggcta agaacatctt
     2941 tttgacttga tggaattttc cttcttggat cgtcaataaa accgtacttt ggtgcttgtc
     3001 tgctttgagt atttttaaat cagccggctt acattgatac ccatcagcga atacgattcc
     3061 attttcaaat gctgttacgt ctgccggtgt caccctttcg ttcacaacta cttgataagt
     3121 ctttgaaacc tttttttctg gaagtagcat ttcatatccc aattgtccat tcgaagtgat
     3181 cagcaatagt cctgttgtat ctctatctag tcttcccact ggatataatg gttggaaacg
     3241 atcggcactt gcaatcagat cgattactgt cgtcttttct ggatctgata cagctgtaac
     3301 agctttttca ggtttattga gaagataata ggactcattt gtttctaagt gttcacctgc
     3361 tacttcgatt tgatgcagta aactatctac atttcgattt tgtcgaaatt cgactgtgcc
     3421 atctatcatc acttgcttca aagcaaacag ccgcttcatt tccttgcgtg tcgtttctaa
     3481 tttttcttct attaatttat ctaaacgcat gctgtttcct ttcttattac tactaattaa
     3541 ttataccttg aatattcttg cttgcaaata catcatcata gttattcctt gacttaatta
     3601 acagttcacg ttacggttaa atagtaaagt gaattggatt tctcatacac agcgtaatga
     3661 cctggtggag aaaagttgaa tcctgacttt ctaaggggag atgcgcgcat ctctcctttt
     3721 gcgctttttt aagcgccctg ccttcgatga tgtgcacgag attagccttt agaattcact
     3781 taaataaaat ctgtccgtta ccaaagcaga cagtcacatt ttaaggagga attttggtta
     3841 tgtcagtatc attagaagta agtaaaagag aagtacgtcc ccgttcatta agaaacaaac
     3901 ttcgtcacga agggaaagtt ccagctgttg tttatgggta taaagtagaa agtaccccta
     3961 tctcattcga tggtttgaca ctaagcaaat tgttgcgtga acatggagcg aacacagtca
     4021 tcacgatgga catcgatgga caaaaagtca atacattggt tcacaagatc caaacgaaca
     4081 cttttaccaa tcatttcgaa catgcagaat ttctatctgt aaacatgtcg gaagaaacag
     4141 aagtcgaaac agaagtcgtt cttgttggcg aatcagtggg tgctaaatct ggtggagtgt
     4201 tagctcaaaa cttgtataca gtagtcgtat ctgctacacc agacaaattg cctgaacgaa
     4261 ttgaagttga cgtgacaaac ttggaattag gtgattctat tacagtagcc gatcttccaa
     4321 aaaacgacga ttacaaattc gtgaccgaca gtgaagagca aatcgctgct gtagtcgctc
     4381 aaagtgaaga agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag
     4441 aataatactg aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc
     4501 ttttttattt ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca
     4561 aacgaaaaat cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa
     4621 atttatactt tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga
     4681 atgaaccctc gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa
     4741 cattcgttga ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc
     4801 gttacgcttc agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt
     4861 ttattattga cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt
     4921 tgatcgaaaa tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa
     4981 tggatattga tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg
     5041 atgacggtac gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata
     5101 gataaacgtg gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa
     5161 ttcttggctt ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca
     5221 ggataaacgc tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc
     5281 gatttaacaa aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc
     5341 ggaaatgttt ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta
     5401 ggtaaaaatg cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc
     5461 tttgcccaag gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa
     5521 gattatcggg gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact
     5581 gtgattttaa ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact
     5641 cctcctgaaa tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt
     5701 gcatcagtca gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca
     5761 ccactattct ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt
     5821 atctattttg gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct
     5881 tccagcgtac tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa
     5941 aaagatttca aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg
     6001 gcgtttcaat cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt
     6061 ctagggacag atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat
     6121 cagccgatga tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg
     6181 aaagaatatg gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt
     6241 agtctcttag caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt
     6301 agcccatcag aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct
     6361 ctttactgga tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa
     6421 agcaagatcc ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc
     6481 gttcttacag gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc
     6541 ggttctgttg ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg
     6601 ggtgaagagg aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa
     6661 atcgttgcgg ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca
     6721 ggttttttag ataacaaaaa ggctgtgaca aaaa
//
LOCUS       DAZKCN010000120         6631 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000120 DAZKCN010000000
VERSION     DAZKCN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.124"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..1035
                     /locus_tag="ACG8K3_002425"
     CDS             25..1035
                     /locus_tag="ACG8K3_002425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9146245.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1104..2048)
                     /locus_tag="ACG8K3_002426"
     CDS             complement(1104..2048)
                     /locus_tag="ACG8K3_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9146246.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2032..4752)
                     /locus_tag="ACG8K3_002427"
     CDS             complement(2032..4752)
                     /locus_tag="ACG8K3_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9146247.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4749..5969)
                     /locus_tag="ACG8K3_002428"
     CDS             complement(4749..5969)
                     /locus_tag="ACG8K3_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP9146248.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6250..>6631)
                     /locus_tag="ACG8K3_002429"
     CDS             complement(6250..>6631)
                     /locus_tag="ACG8K3_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFP9146249.1"
                     /translation="IEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNI
                     EIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMK
                     KADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         1944 a         1272 c         1082 g         2333 t
ORIGIN      
        1 ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc agctactagt
       61 gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga tatcgcaacg
      121 atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact tgaatcatcg
      181 aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa atatggcgat
      241 aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc attaggtgat
      301 acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa agaatatatt
      361 cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac agatgacgat
      421 ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat caaattatca
      481 agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga tttctctaaa
      541 ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa agttaaattc
      601 gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa attgaaagac
      661 ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac agaatactac
      721 gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata caaagatcaa
      781 ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac taaagtaatc
      841 ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga aaatgtgttg
      901 agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac agcttcaaca
      961 aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga agaaacaact
     1021 gattcatcaa aataaaagaa aacaaaaaaa gcgaagactt atgtctcgct ttttttgttt
     1081 tgaatgaaaa aaataaattt ttgctataaa tcttttggta catagaaact tcggttgtcc
     1141 ataccgaaaa tccgatcggt gtattttcct ggttctgttt gacgaatcga agtcaacatc
     1201 atctgcatcg aagcatcgat attgtcgatt tgatgtaata tttctgcttc cattattctt
     1261 ggacgtacag gtgagccata ttctaataag ccatggtgtg ctaaaaccat atgacgtaat
     1321 acgagaacat cttcgtcagc ttcgctgatt ttcaacgcaa gacaggcttt agttatttct
     1381 tcgtcaatca ataccagatg accgatcaaa tttcctgcaa gcgtatattc tgtcgacata
     1441 ggaccggaaa gttcaatcac ctttcctaaa tcatgcaaaa tgataccagc atacaacaat
     1501 gctcgattca gctcaggata ttcatcggcg attgatttcc ctaagtgaag cattgtcaaa
     1561 gtatgataag ctaatccact ggcaaatgca tgatggtttc tttttgctgc cggaaactca
     1621 aaaaattgtt tttgatattt attaagtaga tagcgaacaa tacgattcca atgcgcattg
     1681 gtgatctcaa acaacaattg attgatttct tcttccattt cttctttctt caaaggggct
     1741 ctttccatgt ataaactagg ctctcctggc tcatctaccc ttgttaagcg catatgaaga
     1801 atctttactt gagggtttcc ttgataaacc tctcttttac cgtttagaaa aacgactgtg
     1861 ccggctgtaa atttattgat ttcctcctcc gaagcatccc agtatttacc atcgatagta
     1921 cctgaagtat cttggaaagt aaaagcaata aatttttttc cgtttttagc gattcttaca
     1981 tctgcacttt tgatcaggac aaattgttca aactgctcat cgattactaa atcacgaatt
     2041 tttttcatta tttgctcctt ctaattgttt tacgatttgg ttcatctcat ggtaatagct
     2101 taccatttct ttatcagaag aaaaacaaat tatttgatac ttttctccaa atttagctaa
     2161 aagttcagct aattgatact ttcgctgatg atcataatga agccagccat catcaatgat
     2221 gatcgggcat atcgaacggt tagcttctaa tgagaggtaa gcaaatctca tcgccataat
     2281 cagctgatct ttcgtacctg tagataactc atagatggga aattctgtat atccgtctgt
     2341 taaagtcagt gtatcatttt tcagtaatat cttctgataa tttccttttg ttaaaatacc
     2401 taaataatca cctgctgctc ttaacaactg gggtaattgt ctttcactca tctctgttga
     2461 aagatcacct aagaatgtcc cggcgatttc aagtgctccc cattcctcca gtaattcctg
     2521 taattttgct ttttgccggc tagcttgttg ataaagttcg tccaatgtgc cgtctctttg
     2581 caactcttct acttgaagtt ggagtcgttg ccgattttca cttgcttctt tttcttcttg
     2641 ctggagttgg gattgctttt ctttcagttg gcgcagtctt acagataatt tctcttttgt
     2701 cagctcttca ggaaatagtg gagtaagcat ttccgataat tcattgtatc ttttcatctg
     2761 ctgttcggct tgtttggctt tttgtaaaaa gacaggtact tccgaaggat aagataagcc
     2821 tgcttgagtc aattgttcgc tgtattcttg tatccatact ttttgtttct ttttcaattg
     2881 attgatttct tgcttcaata aagtattttg ctgatagctt ctggcaaatt tcatctgttc
     2941 catctcttgt ataaaacggg aaagcaatac cattttttct gaaattgtct tatcttgtat
     3001 cggtaaccat tctttaacaa actcaaattt ttggtcgatt tgtcgtagtt tttcttgtat
     3061 ttcctgttct cttttatgtt tccgctcaaa tacgccatat aatttctgat aatctatgac
     3121 ttcctgtcca tattctttca tgagtttgag cgtatccatc tttcctaagt gccgatgatc
     3181 cgcaaaacga tatacttctt gctgaagaat ctgtttttct tctccagttc tcttttgttt
     3241 ttcttctgct tcagctaatt gagctaaggc tagatcaagt tgtcctagtt tttcttgcca
     3301 cattcctttt atctcagaga aatcattttt agttttaatt tttggttgcc ttttgtagaa
     3361 gaaatagcca atcatgcctg caccaattag taaaaggatc actttcaagg gcattggtac
     3421 aaatatacct gcaatgatta ttgctccgcc agccatcagc atatacggtt tagatgaaga
     3481 gactggtgct atctctgact gattttgtaa taatgctgga taggtctctt ccaagtcatt
     3541 cacttctgct tcaatcatct cttgctgttc tttagctatt tggagacgca cagaaatctg
     3601 gctttctaga tcagcaatcc ttctttcttc agccagaaga tgcttccatt cttcttcata
     3661 taaaaattct tccggtaacg gatttttctg ccaatgccac ctttgttcca aaatcttcaa
     3721 ttctgaattt gtttctctag tctcaatttg taatctttgc cattcttcgt ttaattgttc
     3781 cgctgtaaat tgaagttgct ggagatcttt tagttcctgc tcctgctcta aataaaaata
     3841 atatctggct gactgctgat ccatcccgct atgcttttcc agtgttgctt ctaattcttc
     3901 caaacgctca tttgtttgct gataccgttt ggagaaagac tccagatcta aaagaagttg
     3961 ctcatcagga agacctgtag tgctgttttt tttgagagat tgccattctt catataaaga
     4021 aaaattaagt tcttgctgtc tggctaatga aaattgtttg ttcgcctctt ttaattgtgc
     4081 caataactgc tgctgttgcg actcgtattc ctgttcttgt tggatcaact tgccaaaata
     4141 agcttcctgt tcttgttttt gatgaatttt ttcttgcagc tctttccatt catttaattt
     4201 ttgattgatt agtaattttt ggcctttttt cttgaataat tgttgtgctt tctgataata
     4261 ttcttgccgc ttttggaata cttgagaact gcccgtgatg cctaaagaaa tcagcgcatc
     4321 atgtagctct ttttctctca aatgatctag ttccatcaac tgctcttgct ggaaagtaaa
     4381 tacctgctgg aataactctt tcgtcaaagg tgcgatcagt ttttctagta actcatcact
     4441 tcccacttga tcccccagcc acaccttcga ttttccgcga ttttgctgct tgtagcgctc
     4501 gacagcaaat tcaccatata caggatgttt aagccatatt ttgccgccat aggctgagcc
     4561 gtcttttggt gtataatcct tttttttacg gccttttgaa gggaaaccaa ataatattgc
     4621 ttgaataaat tgataaatcg ttgattttcc tgcttcgttt cccccataga tcagctgatt
     4681 gcctgaataa aagtcaaaac ttttttgacg ccattttcca aaagcagcaa tctcgatcct
     4741 taaaatcttc attcctgatc ctcctcaaat aaaaaatctt gattaagcag ctgcttggct
     4801 ttttcaagtg tttcttcttg atattccggc agttcgctca atattctagc cgcttcttga
     4861 tgactatata cttcatttaa aatttgttgg aaatcttgag gtgtctgata gtattgaaat
     4921 aactgttcca tcaatttagc agaagcagca agtggggtct tcgtataaaa agtattctct
     4981 atgagagaaa tacgccaaat aaagaaatca tttatcctat ctgagaattc ctctaacaaa
     5041 tagtctaata gctctcctga ttgtatccgt tccaaaacat ctgtattgag atggtcataa
     5101 tcttttaatt ttatctcaat taaactaaat ccatcctcta cttttgaaag ctgctgacga
     5161 atgtgaacaa tgacctcttt tgtcgttctt gctttcctca atgatatttc ctttgtcttc
     5221 caatagacct ctgctacttt aatcggatgt atttggcaac ttcctttact taattcaact
     5281 agtagaatag atgtagaagc ttgttccttt ttagtatgac cttgaggcgc acctggataa
     5341 acaattgttt gcttttcatt taagaccgtt ggcacatgga tatgccctaa agcccaataa
     5401 tcgtatccct tttcttgcat ctttgagggt tggaaaggag catagtttcc tttttgagag
     5461 acgccaggtt ccccgtggta caacccaata tggtaatctg tgagttctct agaaggaaat
     5521 tccatcactt tgtctttttg aatccacggt tgacgataac taaatccact gagagagaca
     5581 gtttctccac tttttatcgt tatttttttt gtctctaccg tttcggatgt aaataaatgg
     5641 acatttttag gaaaggagaa ccaataccgc tctttttgat agaaatcatg atttccgaat
     5701 attataaata caggtatatg gttcttttct aaccgtttca tctgttctga aaaatgtttc
     5761 tgtattttta atgaaggacg attttgatga aaattatctc ctgcaagtaa aacaaaatca
     5821 acgttattta ttattgcctg ttcaatgata tttgataaaa cggtcagatt cgtcttcaat
     5881 agtttttcct gtacagtttt atcaagagtt gtcagtcctt caaatgaccg atccatatgt
     5941 aaatctgccg catggataaa tttcaccatt aaaaatttcc tccttttttt cttttatcat
     6001 accattttca atgttttttt tcatcaataa ccgaaaattt gttcgtatta tgcaaagaaa
     6061 ttacgataaa tggccgactt caaaaaataa gtttattcga aaaaaagatt gtgacatgat
     6121 ttttgtcaca atcctaaata tctatgttat attcgcttcg tttgacactg aagaagtact
     6181 atttgtactg gaatttatgc tccatttttc cgtatttaga attctatgtt ctatactacc
     6241 ttaattagtt tatatggtaa gaattctttc aaatgcttga gatactccgt ctttatcatt
     6301 tgaaacagtg acataatcag cttttttcat gatttcttga tgactatttc ccattgcaaa
     6361 actaatatct gcactttcaa acatagataa gtcatttaaa ctatctccga tcgcgattct
     6421 caaaggtttc tttaaattgt agattgattc caaacgatca aacaccacac ctttagatac
     6481 attttgaggt acgatttcaa tatttacagg tgccgattca gtaacagaca catttagcat
     6541 ctttttcaaa tggttaaata tattagcttt caattgacga ttaggaataa aaataaaaaa
     6601 tttgtaaatg ctgatagcat ccttttctat a
//
LOCUS       DAZKCN010000121         6610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000121 DAZKCN010000000
VERSION     DAZKCN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.125"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..512
                     /locus_tag="ACG8K3_002430"
     CDS             132..512
                     /locus_tag="ACG8K3_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9146250.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            608..1117
                     /locus_tag="ACG8K3_002431"
     CDS             608..1117
                     /locus_tag="ACG8K3_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146251.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            1194..1373
                     /locus_tag="ACG8K3_002432"
     CDS             1194..1373
                     /locus_tag="ACG8K3_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146252.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            1428..1991
                     /locus_tag="ACG8K3_002433"
     CDS             1428..1991
                     /locus_tag="ACG8K3_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146253.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2038..2553)
                     /locus_tag="ACG8K3_002434"
     CDS             complement(2038..2553)
                     /locus_tag="ACG8K3_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP9146254.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(2579..3448)
                     /locus_tag="ACG8K3_002435"
     CDS             complement(2579..3448)
                     /locus_tag="ACG8K3_002435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9146255.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            3574..4254
                     /locus_tag="ACG8K3_002436"
     CDS             3574..4254
                     /locus_tag="ACG8K3_002436"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9146256.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4407..5387
                     /gene="pta"
                     /locus_tag="ACG8K3_002437"
     CDS             4407..5387
                     /gene="pta"
                     /locus_tag="ACG8K3_002437"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9146257.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            5510..5983
                     /gene="tsaE"
                     /locus_tag="ACG8K3_002438"
     CDS             5510..5983
                     /gene="tsaE"
                     /locus_tag="ACG8K3_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9146258.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            5976..6500
                     /locus_tag="ACG8K3_002439"
     CDS             5976..6500
                     /locus_tag="ACG8K3_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9146259.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
BASE COUNT         2187 a         1172 c         1393 g         1858 t
ORIGIN      
        1 cctactttct gttttttttg ccttttttct tataaaaagg aaaaacagct atttttttca
       61 atctgacaca tttacaatgg aactggagat taatatagat ccgagcttaa acgaagaata
      121 ggagtgctca tatgaacctt acaacgatcc atcatatagc catcattgtt tcagattatc
      181 agaaatcacg tgatttttat gttaataaat taggatttga agtaatcaga gaaaattacc
      241 gcaaagaacg agatgactat aaattagatc tgaaactagg aagtgctgaa ttggagattt
      301 ttggaattgc taatgcgcca caacgaccaa actatccaga agcttgtggc ttgcgtcatc
      361 ttgctttcaa agtagacgat attgaagaaa caattgcaga attgcagtcc ttagggatcg
      421 aatcagaacc gattagagaa gataccttta caggaaaaaa aatgacgttt ttctttgatc
      481 cagacggatt gccattagaa ctgcacgaat aaagataaca aaattgtcac ttttttttcg
      541 tttcattcga tttttcattc gttcatttct tgatattctt taaaaaaaag aaacaggaga
      601 atgaagaatg aaaaaaatga tgtttggtgt tgctgccctc atcgtaatag ggattaccgg
      661 tatgacggga ctgctcaatg ccgcttttca catggatgat attagtgacc aggaatatcg
      721 gatctcattg aaaaaagcag cagcaatcgt aaaaaacgaa acaaaagaac ataacatatc
      781 tgctattcat tttgcagttg aaaaagattt ggagaatcct acgaatgcaa catatgaata
      841 ccagtttttt tgtgaaggac aagtcatagc agtcaatcca tccactggga aaacaaccgt
      901 acgaaatgac acggaaaagg ccgcagaaca tctttttgat atagaaaaaa cggaagagtt
      961 caaacaaccg caagcagcga tgaaggaagc agtcagctac tacgggaaaa aagcaaaagt
     1021 gaaagagtgg acacttctag tcaaagatga acgtccttat tataaagtcc gattaataga
     1081 tggagaacag gaccgtcagg tatggatatc tgcatagaag aaggatattg cttctctttt
     1141 ttacaatata agagtaagct aagagagtaa agatgaaagg aagttgagat aacatggaaa
     1201 aaatgaaatg ccctaattgc ggaaaaaaat ttgcatacga agaagtcaat aatgtagtcg
     1261 aacataatga taaagaaatg ccagtcgtct gcccttattg ccgaactgaa gcggcaagaa
     1321 tcgttactca tggttatttc attactgaaa agattgaaga ttttttaaaa taacgatttt
     1381 gcgatttctt tttctgcatg ttagaatgga gaaaaggaga ggaaactatg attcgttttg
     1441 cgagaaaaga agatgctcca gaaatcgccc cattgatctt agtgattcta aaagatatgg
     1501 aactgccatt tttgaagcag acaggggaac aaaagacttt agagatttta gaagaagcaa
     1561 tcactgatcc aaattatcgt tacagttatt cacgaggtat cgtagaagaa atagacggta
     1621 aagttgcagg agttgcttac ggttatacag atgaagaaga atcaatcatc gatcagccac
     1681 tggtacctat cttagagaaa tatcagctag atgcgtctac acgtttgttc attgataaag
     1741 aaacgtttcc caacgaatgg tatttggact ctatcagtgt atcagaagat tttagaggac
     1801 aaggcatcgg ttcgcgatta ttagaagcat tacctaaatt ggcgaagaaa gctaatcgct
     1861 ccgtcatcgg tctaagtgtg gatgaaaaaa atccaaaggc taaaaaatta tacgaacgcc
     1921 atgacttcaa agttgtcggt cagcggatga tcagtggtca tctttatgat catatgcaaa
     1981 agaatatcta aaaagatgta cctgaaatgc gtatttccgc atttcaggta catcttttta
     2041 acttaagtta agtcggtaag aaacaggctt atcgcgactt ttgtctttac ggtagagcca
     2101 gtaatctaaa accgcagcaa tacaaataca aactggagca tcttcatcat ttgagacatc
     2161 caatacatag taatctcctg aaaaaagtgt tgctttatcc attttcatga tttgctgatt
     2221 gaaatgatgg atttgatatt gatgattata gatatcacct tggactgtcc agtgaagtct
     2281 acgaatataa taaaagtcac cgggccaatt gaatattttc tgcatggttc ccaccttttc
     2341 gaactcttta tacagctcaa aacgcgtacc gaatgtcagg cttatctgct tgatgtgagc
     2401 aacgagatcg ccattcatgg cataaagaga caatgcatcg ccctttgttc cccagcgacc
     2461 aaccatcaaa aacagggaat gtcctttttc gtttttgaca attgtccgag ttgcacgtcc
     2521 aagctgcttt tcctgaatga aaaattctga catctatatc cctcttttct agataaaact
     2581 acaagtcttc tgcaatcgcg cgcaatatgg cttttcctac gccagattca aggtttgtgt
     2641 ccgttgtata tttggcataa tcttttactt cgatttctgc atttcccatt gcgaaactga
     2701 ctcctgctac ttgcagcatg ctgacatcat tgaagttatc gccaatcgtc atgacttctt
     2761 ctaaagaaat cccacgctcg tgagcgacat gggcaacagc aattcctttt tgagcgtttt
     2821 tatggttcac ttcgatattg ttttgaccag aggaagtaac ggctagatcg cctaaaagag
     2881 aaatctgctt tccaactgga cctaatacag ctggcccttc tgtatgaaaa caaataattt
     2941 ttaaaacttc cagattcttt tcttccaaaa tagcacgaat actgtccacg tatgtgatgt
     3001 gcaataactc cagcttagct gaggccatgg caatcgccat tttatacgta aggtgaggta
     3061 aatgggtagc caccatggaa gcaaaacttt caattcgttt cgcttgattt tcagaataaa
     3121 gaccatcgtt ggttgctact tcatagtaaa taccgttagc atctagaata tctaaaacag
     3181 agattgcttg attatttgga atcgacacgg taaacaatga atttccttct ttatcaaata
     3241 cctgtgcccc gttcaaagtg atcatcgcac aatcgattcc tacctcatct aaagctgcgc
     3301 gagcttcttg atagtttcga ccagtagcga ccatgaactc aacaccggca tcatttgcaa
     3361 aacgtatcgc ctcggcattt tcaggagaaa cactcatatg ggcatctaat aatgtgccgt
     3421 ccatatctga tgcaattaat ttgatcataa tgattatcgt tcctttctgc ttcttgttcc
     3481 tagtttacca tattaatcaa gtgctgaggg tgaagattgt cccttgtgat tgaagaaaat
     3541 gtgagttatt ctttttatta ggaggtggag tttttgaaag aaatcattca taacagttgg
     3601 caagaagttt tgtcctcaga attttcaaaa gattattatc tgcatctaag agaattttta
     3661 aaaaaagaat atgcgtccca aaagatccat ccagatatgt atcatatcta tgaagcattg
     3721 gaattgactc cttatgaaga agttaaagtg gtgattttag gacaagatcc ttatcatgga
     3781 gaaaatcaag ctcatggctt atctttttct gtgcaacctg gcgtaaagat cccgccatcg
     3841 ttaagaaata tctataaaga gctttatgat gatcttggaa tcgcacctgt tcaacatgga
     3901 aatctagtgt catgggctaa acagggagtc cttttactca acaccgttct taccgttcgg
     3961 gaagggcaag cgtattctca tcgcgggaaa ggttgggaaa gattaacaga cacgatcatt
     4021 gaaaaattaa atgaacggga aaaaccaatc gtgtttatct tgtggggaaa accggcacaa
     4081 gaaaaaatca aaatgatcga taagagtcgc catatcatca taacatctcc acatcccagt
     4141 ccgttatctg caagtcgtgg cttttttggt tccaaaccct tctcgaaaac gaatgatgcg
     4201 ctccttgcgt taggagaaga accgattgat tggcaactgc ctgaaacagt gtaataaaac
     4261 agcgctttcc tgttatagtg cagttcgctt tgtatgtgaa aaacttaatt tttccagcat
     4321 ttttgactga tacagataaa ataaactgtt ctttttttct ggaatagtgt atgataatac
     4381 atataaaaac gtctggaggt catcttgtgg aattgtttga tagtttaaga tttaaaattg
     4441 ttcgccgtgg gatcaaaatc gttttcccgg aagctactga tgctcgtatt ttaggcgcag
     4501 ctgcccggtt gaaagcagaa gaacttgtgg aacccatttt agtagggaac gaagaagaaa
     4561 taagaaatgc agcgcatgcc agaggaatca aaacatctag ctttacgatc atcagccctg
     4621 ataattacga taagtgggat gaaatggtgg atacttttgt tgaacgtaga aatggaaagg
     4681 catccaaaga acaagcagaa aagattttaa aagatgtgaa ttactttggt acgatgctta
     4741 cgtatatggg gattgcagac ggaatggtct ctggagcgat ccattcgact ggcgacactg
     4801 ttcgcccagc acttcagatc atcaaaacaa agcctggcat cagtcggaca agcggagcga
     4861 tgatcatggt acgtggaaga gatcaagaaa aatatgtctt ttcggattgt gcgatcaacg
     4921 tgaatccaac tgcgcaagaa ttggctgaaa tcgccgtaga ttctgcaaaa acagcagaat
     4981 tgttcgatat cgaaccaaaa gtagcaatga tgagcttctc gacaaaaggc tcagcgaaat
     5041 cgccagaagt agacaaagta gttgaagcaa ctaaaatcgc taaaaaacta gcgccacaat
     5101 acatgatcga cggcgaattg caatttgatg cttcttatgt tccttcagtt gcacaactga
     5161 aagcgccaga ttcagaagta gctggtcaag caacagtctt tgtatttcca gaattgcagt
     5221 caggaaatat cggctataaa attgctcagc gtttcggtaa ctttgaagca atcggaccaa
     5281 ttctacaagg cttgaacaaa ccggtttctg atttgtctcg tggagcaaat gaagaagatg
     5341 tatataaatt atcgattatc acagcagcac aaacattgat tgattaacaa atacacgcga
     5401 actagccaaa aacagcagct gctgttcttg actagttcat tttttcttta aaaaaacttc
     5461 attacgcgat atactggcaa aaagaaagaa gagcgggaga agatgaaaaa tgatcgaact
     5521 atcaggatta acaatgacag aacaattcgc agaggcaatt ggtcattccg caatgccagg
     5581 tgacaatctg atattgactg gtgatctagg tgcgggtaaa acaacgttga cgaaaggaat
     5641 tgcccaagga ttagggataa cgcaaatgat caaaagtccg acatacacga ttattagaga
     5701 atatagtcaa ggtcggattc cgttgtatca tatggatatt tatcgggtag cagctagcgg
     5761 agcagattta gggttagatg aatattttga aggtgatggg ctttccgttg ttgaatgggg
     5821 aaatctatta gaagaagcac tgccggagga ttatttggaa ctgattttag aaaaaagtga
     5881 tacagattta gaaaagcggt atgtgaaact aatctcatac ggggagcaaa gtgaaacctt
     5941 caaacagcga attctagaaa aatgggctga aaatcatgag tgaagaagtt gggatcatct
     6001 tgcgggaagc agtgccagga gatgcaaaag acattctttt gatgatggga caagttaata
     6061 aggagacaga atttttagtt ttggatgaag cagaactcct gttaccacca gaaaccttag
     6121 aagaagagtt ggattatata tacgaaagca ataataattt attgctgctt gcaatctatg
     6181 aagggacgat tattggaaca gcatctgtca aggcagacag tcaatttcgt ttgtctcacg
     6241 tgggagaagt cggtatcagt attctgcagg aatattgggg aatgggacta ggcactctta
     6301 tgctagaaga aatcatcagt tgggcgaagg aaatgggtgt actttttcga ttggagcttg
     6361 acgttcaagt tcgtaatgaa agagccgtac acctttaccg aaaaatgggc ttccaaatcg
     6421 aagcggtcat gcctagaggg gcaagaacag atctaggtga atttttagat gtatataaaa
     6481 tgagttattt gattgaatag aagaaatagg aaaagagtga gaaactgtca tccttttgag
     6541 aaggcagaaa aacacacgaa gggactgtga caaaagtcgt gtcgcagtcc attttttttg
     6601 aataaacggt
//
LOCUS       DAZKCN010000122         6229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000122 DAZKCN010000000
VERSION     DAZKCN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.126"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..1830)
                     /locus_tag="ACG8K3_002440"
     CDS             complement(151..1830)
                     /locus_tag="ACG8K3_002440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP9146260.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(1832..2044)
                     /locus_tag="ACG8K3_002441"
     CDS             complement(1832..2044)
                     /locus_tag="ACG8K3_002441"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP9146261.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            2437..4167
                     /gene="efrA"
                     /locus_tag="ACG8K3_002442"
     CDS             2437..4167
                     /gene="efrA"
                     /locus_tag="ACG8K3_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP9146262.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4164..5924
                     /gene="efrB"
                     /locus_tag="ACG8K3_002443"
     CDS             4164..5924
                     /gene="efrB"
                     /locus_tag="ACG8K3_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP9146263.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            6019..6216
                     /locus_tag="ACG8K3_002444"
     CDS             6019..6216
                     /locus_tag="ACG8K3_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146264.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         1884 a         1110 c         1306 g         1929 t
ORIGIN      
        1 atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca
       61 aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca
      121 ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg
      181 caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat
      241 cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg
      301 gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc
      361 acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac
      421 agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat
      481 atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg
      541 cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc
      601 aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg
      661 gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc
      721 accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc
      781 agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga
      841 aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg
      901 gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc
      961 aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc
     1021 taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg
     1081 accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct
     1141 ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt
     1201 tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa
     1261 gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt
     1321 gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat
     1381 ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa
     1441 acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc
     1501 atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc
     1561 ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa
     1621 ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat
     1681 tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg
     1741 gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc
     1801 tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat
     1861 tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg
     1921 gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag
     1981 attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga
     2041 tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga
     2101 caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga
     2161 ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta
     2221 ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag
     2281 tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa
     2341 tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa
     2401 tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc
     2461 atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt
     2521 gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat
     2581 tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt
     2641 ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt
     2701 cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc
     2761 gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa
     2821 aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc
     2881 atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg
     2941 gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta
     3001 gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc
     3061 gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt
     3121 tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat
     3181 atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt
     3241 gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta
     3301 tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa
     3361 gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact
     3421 aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca
     3481 gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt
     3541 accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa
     3601 ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat
     3661 aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg
     3721 cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa
     3781 gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt
     3841 tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt
     3901 ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag
     3961 ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt
     4021 gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt
     4081 atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct
     4141 caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc
     4201 gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag
     4261 tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta
     4321 atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca
     4381 cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg
     4441 tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc
     4501 cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg
     4561 tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg
     4621 gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta
     4681 tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat
     4741 attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga
     4801 caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa
     4861 aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga
     4921 tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct
     4981 tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt
     5041 ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat
     5101 cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag
     5161 tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg
     5221 catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa
     5281 ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc
     5341 gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga
     5401 gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag
     5461 gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg
     5521 atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata
     5581 tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg
     5641 cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag
     5701 acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa
     5761 gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg
     5821 atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt
     5881 atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa
     5941 acagtttaaa acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag
     6001 aaaagtgagg acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga
     6061 gaagttgatc gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt
     6121 gaaaatgagt ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta
     6181 aaaaaagtaa tagaagaaaa tatagatgta caataaaaac aggtaaaac
//
LOCUS       DAZKCN010000123         6063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000123 DAZKCN010000000
VERSION     DAZKCN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.127"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..2631
                     /gene="adhE"
                     /locus_tag="ACG8K3_002445"
                     /gene_synonym="adhC"
     CDS             34..2631
                     /gene="adhE"
                     /locus_tag="ACG8K3_002445"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9146265.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2860..3966
                     /locus_tag="ACG8K3_002446"
     CDS             2860..3966
                     /locus_tag="ACG8K3_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP9146266.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3983..5007
                     /locus_tag="ACG8K3_002447"
                     /pseudo
     CDS             3983..5007
                     /locus_tag="ACG8K3_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5013..5492
                     /locus_tag="ACG8K3_002448"
     CDS             5013..5492
                     /locus_tag="ACG8K3_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9146267.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5508..>6063
                     /locus_tag="ACG8K3_002449"
     CDS             5508..>6063
                     /locus_tag="ACG8K3_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFP9146268.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1949 a         1075 c         1368 g         1671 t
ORIGIN      
        1 aacaaataac catgacccat taggaggata atgatggcta aagtaataga aaaagaaaaa
       61 acgaagacaa ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc aaatgcagca
      121 ttgaaagaaa tggaaagctt tgatcaagaa aaagtagacc atatcgtgca tgaaatggca
      181 atggcagctt tggatcaaca tatgccatta gcgaagatgg ctgtagagga aacaggccgc
      241 ggtgtttacg aagataaagc aatcaaaaat atgtatgcgt ccgaatatat ttggcataat
      301 atcaaacatg ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa
      361 attgctgaac cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc gacttccaca
      421 acgattttca aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt tgctttccat
      481 ccaagtgccc aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct
      541 gccggagctc ctgaaaattg tatccaatgg atcgaacatc cttcgatcga agcgacttct
      601 atgctaatga accatccagg tatcgcgatc gtcttagcga caggtggagc tggaatggtc
      661 aagtctgctt actctacagg aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct
      721 tatattgaaa aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca
      781 tttgataatg gaatgatttg tgcttctgaa caagctgtga tcgtggataa agaaatctat
      841 gcagcagtaa aagcagaatt ccaagcacac caagtttata tcgtaaaacc agatgaattg
      901 caaaaattag aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt
      961 ggacattctg cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa
     1021 atgctgattg ctgaattgga aggcgtaggc ccagattatc cgctatcaag agaaaaactt
     1081 tctccagttc ttgccatgat caaagctaac aacacggacc atgcgtttga tttgtgtgaa
     1141 ggtatgttga atcttggagg attaggacat acagcagtta tccattcaga aaacgaagag
     1201 ttgcatgtca aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct
     1261 gcagaaggcg gcatcggtga tatctataac gaaatgattc cgtcattgac tttgggatgc
     1321 ggttcttatg gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc
     1381 aaaactgtag cgaaacggag aaataacatg caatggttca aattaccgcc aaaaatcttc
     1441 tttgaaaaga actctctatt gtacttagag aaaatggaaa acgttgaacg tgtcatgatc
     1501 gtttgtgacc ctggtatggt ccaattcggt tactgcgata cagttcgtga agtattggca
     1561 cgacgtaaaa atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca
     1621 aataccgtat acgcaggaac gaaactgatg gcagacttca agccagatac agtaatcgct
     1681 cttggaggcg gttctgcaat ggacgcagct aagggaatgt ggatgttcta tgaacaccca
     1741 gacacatctt tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg gacatataag
     1801 atcgacaaac cagaaaaaac acaattcgta tgtatcccga caacatcagg gactggttct
     1861 gaagtaacgc catttgcggt catcactgac agtgaaacac atgtgaaata tccgttagca
     1921 gattatgcat tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca
     1981 gcatctgtca cagcagatac cgggatggat gtgttaacac atgcaatcga atcctatgtt
     2041 tcagtcatgg cttcagatta tacacgtgga ttgagcctac aagcaatcaa gttagtcttt
     2101 gactatttag aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa aatgcacaac
     2161 gcttctacaa tggctggtat ggcgtttgct aacgcattcc taggtatttg tcactcagtc
     2221 gcacataaaa tcggcgggga atacgggatc cctcatggac ggacaaatgc gattctttta
     2281 ccgcatatta tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat
     2341 gactatttcc gtgcagatac agattacgca gatatcgcaa aattcttagg tctgaaaggc
     2401 gaaacaacag aggaattagt cgaagcttta gcaacggctg tttatgaatt aggaaaatct
     2461 gtcggcatca acatgagtct aaaagctcaa ggagtcacac aagaaacact agatacaacc
     2521 gtagaccgta tggctgaatt agcttacgaa gaccaatgta cgacagctaa tccgaaagaa
     2581 ccattgatct ctgaattgaa acagatcatc attgatgctt acaacggata agcaaaagaa
     2641 agcgtagaaa aaagataaga aaatagatga aaaagaactt ggaaaacgtt gtttctccaa
     2701 gttctttttt taatttccaa gtaactgaaa gttacttttg ttatttaaca tttaggtcaa
     2761 ttttagacag tttttgttca aaaagcgata tttatagtgg acggtattac tgctatatta
     2821 aggatataaa atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt
     2881 tttgtaccta aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg
     2941 atcaaagacg ataatggtga atttttgttg gactttgatg gactgaaagt agatgacaaa
     3001 agctggacag tgtggaattg gccacaaggt gtcggtctat atggtattta taaaaattac
     3061 cagatgacaa aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg
     3121 gaagaaggcg ctccgcccaa aaacgtcaat acaatggcac cgctattgac tatggcttac
     3181 ttgtatgaag acacgaaaga ttcacgctat ttgccttatt tagaacaatg ggcagaatgg
     3241 gtcatgaatg acatgcctcg gacaaatgaa gacgggttac agcatgccac atatggaccg
     3301 gaaaataaga atcagttgtg ggacgataca ttgatgatga cagtgcttcc tttggcaaaa
     3361 atcgggaaac tgttgaatcg cccagaatat ttagaagaag ctcgttacca gtttatgatc
     3421 catatcaaat acttgatgga taaacgaaca ggattgtggt atcacggctg gacgtttgaa
     3481 ggaaatcata actatgccga agcattttgg gctcgtggaa actgctggtt gacgattgct
     3541 attccagaga tcattgaaat cttagaatta tcgaaagaag atgcattgcg caaattattg
     3601 atcgaaacac ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga aagcggttta
     3661 tggcatacgt tgttggatga tccttcttct tacgtagaat cttctgcaac tgcaggattt
     3721 gcttacggta ttttgaaagc ggtccataaa cgttatttac cacaagaata taaagaagtc
     3781 gcttatcgtg ctatccaagg attgcttgaa caaatcgatg aaaaaggaga agttcaaaac
     3841 gtttcaatcg ggacaggaat gggggacagt ttagattttt atcggaatat cggaatcaca
     3901 gcaatgcctt atggacagtc gttaacggtg ctttgtttga cagaattgct cgtctcttat
     3961 tgttaaaaga aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag caagttcgct
     4021 tagcctaggt ctgctaggtc taagcggctg cggaaataat tctagtgtgg caactgtcgc
     4081 agatgcagac gtaaatgaga tagttacctt gcgctttgct tatgcaagca acagccagta
     4141 attgatgcaa tgaatgaatt tgggcgttta gtaaaagaaa aaacagatgg ttccgtacag
     4201 atccagtatt tcccagatgg acaactgggt ggagaacgtg aactgatcga attgacacag
     4261 tcaggagcag tagatttcac aaaagtaagt gcctcagcgt tggaaagttt ctctaaagat
     4321 tattcgattt tttccgttcc ttatctattc aatgatgaag aacatttctt taaggtgatg
     4381 gataatgaag aaataatgga gccaatctac caatcgacca aaaatttagg atttaccgga
     4441 ttgacctatt atgattctgg tcagcgcagc ttttatatgg tagacggtcc gatccatacg
     4501 ccggaagatt tgaagggaaa gaagatccgt gtgatgcaaa gcgaaaccgc tatcaaaatg
     4561 gtagagcttt taggcggttc gcccgtacct atgggaagtg atgaagtgta cacgtctctt
     4621 caatccaatt tgatcaacgg atcagaaaac aatgaatttg tcttgtatac agctggacat
     4681 ggcggggtag ctaaatacta ttcctatgat gagcatacac gtgtgccaga tatcatcatc
     4741 atgaatgatg ccatcaaaga acagctaact gatgaacagg aaaaagccat tgaagaagca
     4801 gcgaaagaat caacggtttt tgaaattgaa gcttttgcaa aagcaattga agaagaaaaa
     4861 gctttagcac aagaagaaca tggagtccaa ttcaatgatg tagataatac accgtttcga
     4921 gaggcagttg ccccacttca tgatatattc aaaaacgatc ctacctacaa agacctttat
     4981 caaaagatcc aagagcaagg agcgtagctg taatgaaaat gattttaaac aaggtattag
     5041 agatcctggg agctgttttg cttgttggga tggtcgctgt tgttttatgg caagtatttt
     5101 ccagaacggt tttaggaaat ccaaatacgg tcacggaaga gcttgttcga ttcggtcttg
     5161 tctggtttgc catgttgtct tccgcgtatg ttgtgggaca aaaagcacgt ttagccgtaa
     5221 cggttttaag tgaaaaatta actggcaaaa aaagtaaaat tttagaagtt gttgttcaaa
     5281 gtttatttct ggtatttgct gcgatgatca tggtgtatgg cggatggaat gctgtcacat
     5341 tgacaatggg gcagatctcg ccttctttaa gtattcctat gggatatgtt tatctgtcag
     5401 tacctgtatc gggggtactc atcattatct atagcctgat caatctaatc gatacattaa
     5461 gcaacaagcc agcaattcaa gaaaaaatat aaaaatggag gaataaaatg gcaatagaag
     5521 ccggattagt gttatttttt gtatttgttg tgttgcttgt attagggatg ccgattgcta
     5581 taagtattgc attctcatcg atgatgactt tattgttagt tataccgttt gatgtttctg
     5641 cttttagttc agctcaaaaa atggtgggaa gtatcaatag tttttcgctg atcgctattc
     5701 cgtttttcgt tctctcgggc attatcatga ataatggagg gatagctaag aaactagtgg
     5761 attttgcgat gttgttcgtc gggcgcgtgc cgggggcttt ggctcatacg aatgtgttag
     5821 ggaatgcttt atttggttcc atctctagct cggccattgc cgcttctacc gcgattggtg
     5881 gagtgttgat ccctcagcaa gtggaagaag gatatgaccg gaagtttgcg acagcggtca
     5941 atatcgcgtc agctccgaca ggtatggtca ttcctccaag tactgctttt atcatgtatt
     6001 cacttgttgc tggtggtgct tcgatctctg ctttgtttat gggaggttat ctcgtaggcg
     6061 ctt
//
LOCUS       DAZKCN010000124         6036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000124 DAZKCN010000000
VERSION     DAZKCN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.128"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(535..1038)
                     /locus_tag="ACG8K3_002450"
     CDS             complement(535..1038)
                     /locus_tag="ACG8K3_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9146269.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1397..2422
                     /locus_tag="ACG8K3_002451"
     CDS             1397..2422
                     /locus_tag="ACG8K3_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9146270.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2769..3647
                     /locus_tag="ACG8K3_002452"
     CDS             2769..3647
                     /locus_tag="ACG8K3_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP9146271.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4123..4239
                     /locus_tag="ACG8K3_002453"
     CDS             4123..4239
                     /locus_tag="ACG8K3_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP9146272.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5167..5505
                     /locus_tag="ACG8K3_002454"
     CDS             5167..5505
                     /locus_tag="ACG8K3_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146273.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5599..5829
                     /locus_tag="ACG8K3_002455"
     CDS             5599..5829
                     /locus_tag="ACG8K3_002455"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP9146274.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            5843..>6036
                     /locus_tag="ACG8K3_002456"
     CDS             5843..>6036
                     /locus_tag="ACG8K3_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146275.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVCFLIS"
BASE COUNT         2052 a          902 c         1095 g         1987 t
ORIGIN      
        1 ggaataaaaa aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac
       61 gacacagaaa ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc
      121 ctttgaaatg ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag
      181 ggagcctaaa taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct
      241 ttttatagaa aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga
      301 ttgagatatt gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct
      361 ttttgttaaa taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa
      421 taatatagta gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac
      481 cgtcatagat acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg
      541 aagacggaca gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta
      601 tcccaaaata atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg
      661 ataggcccat aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc
      721 tacgccaata ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat
      781 ccctaatttt ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg
      841 aaaaaagaca atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa
      901 agtagaaaaa gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt
      961 aaatgatagc ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa
     1021 ttttgataca taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct
     1081 gaaaaatcat aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga
     1141 agcttttttt tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga
     1201 aaagttgcta tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc
     1261 tccaagttgt ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact
     1321 aggtaatgct gacaaacggg attggcattt catcttggtt cagctttttt tccaattact
     1381 aggaaggagg gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc
     1441 ttttcgtatc atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa
     1501 tcatgatttt ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca
     1561 aaacataccg atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt
     1621 tgctttcgtt ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc
     1681 tttggttgct ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg
     1741 cttgtttttt gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt
     1801 accgatcgtc tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat
     1861 tacaaaatta ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag
     1921 atttcacgaa gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct
     1981 ttccgtttat gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg
     2041 tgcatcgatt ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct
     2101 gattggatcg atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca
     2161 gttgcagacc atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag
     2221 aaattatgca gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat
     2281 tcgtggagca tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag
     2341 tttcatcggt ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt
     2401 tgttcgtcaa aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga
     2461 tttttgtcac aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa
     2521 ttcacacaaa aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa
     2581 ttttatattt attctatcaa aaaattttgt ttatgatggt aactgaataa actttcctga
     2641 gatggtcaat gctttccttg aaagtcttac agatattctt tcttcataga aagtttaagt
     2701 tttatagtta gtgtacaata aaagtatgtt tataccatat ggtgtccgga taatcagtca
     2761 tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt gatgaagaaa ataagcaatg
     2821 aaatccaaat tctggcagaa actttaactc ttcccaaagt cgttatagag caaatgattg
     2881 tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata tagtcatgaa ctaatggatc
     2941 ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt ctatgaagag gaaccttttg
     3001 cagcattagc tatttatcta tatgcagctt ctcaaagttg gaaaaaaatt tatcaaccat
     3061 tgaagattcc aagaactatc tatttggcaa caatgaatgc ttttactaga tttattcatg
     3121 aacatttcca agtaacagga agctatcgct ttgaccgtgg tttttggatt tggcattaca
     3181 ctagtggctt gatctttcgg attggtgaat tggagtacga aatgacatat tttccgaaag
     3241 ggtacacgga tctaagatta gaaggaaagc agtgcctttc tcttcatatt ccttccgatg
     3301 cagatttatc tcagaataag ataagagata gttacgaaag tgccattcgt ttttttgaac
     3361 tttattttcc tgattaccat taccaagtga tgtacacaga tacttggtta ctatcaccca
     3421 atcttactaa atggttgaaa aaagagtcaa aaatctgtct ttttgcagcc gattatcgat
     3481 tattatcagt tgatgaacag gacgatagtg gagtcccttg gatctttgga cgagtagatg
     3541 cccaaataca tgattatcca gaaagtacga gtttacagcg acaagcaaaa gagcaattgc
     3601 ttgcaggcga gcacattggc tctggcttag gtattcttac ttcataagtt tagtacttcg
     3661 aaatagttta aaagagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca
     3721 cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     3781 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
     3841 ctctttttta gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa
     3901 tagatattaa agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa
     3961 aatttatttc cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga
     4021 ctttcatttt aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt
     4081 ttttagcatg actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc
     4141 tcaagtgcat accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag
     4201 atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa
     4261 aaaatagccg aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc
     4321 gaaagaactg ctgttgattt tccattttta acatatgata aattggtaga atctttcttt
     4381 tcaaattgat accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg
     4441 tacgtttata aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg
     4501 accagtagta ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag
     4561 tcaagaatgg aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt
     4621 catgtagaag cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa
     4681 agctcccaat atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga
     4741 ttcgtatctc tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat
     4801 gcaaaaaaat aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg
     4861 gttttcagat aatagtcgat aagttgtgta ttggaatttt agatacagta ataaagtatt
     4921 tttcatgaaa ttttgtgaga atcactatta caagtatctt taataaaatt aatggttaag
     4981 gaggaactta aagtagaatg taaatgaata gttgtagtca gtcgattgtg atactagact
     5041 caaagttgca ggaataagtt tttcaatttg tgattggaag ataaaaaatg aaaaagaagg
     5101 gctttttttt atactttgtt gattgtagtg tttattttcc ctctgtttag ccaagtaaca
     5161 ttttctatga ctcaaccagt ttcaagtgag gaacatgata acctttttcg tgctacagaa
     5221 gagtttaaga aatatcagga aaatattgtt ccggagactc cattagctag tgagccaatt
     5281 actgatattg atggaaacca aataggatgg tatatacagt acgaagttat ggtggtaaat
     5341 aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa caccgattga aaaggtgagt
     5401 tcattgttaa cgtatattta tactgatgaa agtgacgaga ctgagatcat tttaacggat
     5461 tactcaaaaa ttgctactga taaaaagtat atattgtgga tgtagacact agtgaaatac
     5521 tttatgatat agaaattgat gaaaatttac tagatgtagc acaagaaatc gatcatcaaa
     5581 agcaggaagc tattcgatat gctggtagaa aagggaaaag aaaatattta ttacgtgaat
     5641 gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt
     5701 aactcgactc cgacatttac agtttacaga gaaggatcga tagaaaaaac tgttttttgg
     5761 actaaagaaa gtggaatgtc tttagctgaa gtagaagagt ttttggatta tgttagtatg
     5821 caacagtaag ggagttctta ttatgaaaaa ttttgacaca tattatgtta ttgttttttc
     5881 gatatctttg ctgttaatat tactaacttt aatgcagtat aaagagtatc cagagtattt
     5941 tattttaact tttgtatatt tagtaagtag ctacacactt ctatataaat tcagaagttc
     6001 ttctagagtt tctaaagttt gtttcttaat tagctt
//
LOCUS       DAZKCN010000125         6009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000125 DAZKCN010000000
VERSION     DAZKCN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.129"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG8K3_002457"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG8K3_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9146276.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG8K3_002458"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG8K3_002458"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9146277.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="ACG8K3_002459"
     CDS             1947..2645
                     /locus_tag="ACG8K3_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP9146278.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="ACG8K3_002460"
     CDS             complement(2813..5452)
                     /locus_tag="ACG8K3_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP9146279.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1689 a         1318 c         1104 g         1898 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggcttttt
//
LOCUS       DAZKCN010000126         5968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000126 DAZKCN010000000
VERSION     DAZKCN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.130"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..113
                     /locus_tag="ACG8K3_002461"
     tRNA            43..113
                     /locus_tag="ACG8K3_002461"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Cys,seq:gca)
     gene            134..218
                     /locus_tag="ACG8K3_002462"
     tRNA            134..218
                     /locus_tag="ACG8K3_002462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Leu,seq:caa)
     gene            280..352
                     /locus_tag="ACG8K3_002463"
     tRNA            280..352
                     /locus_tag="ACG8K3_002463"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:His,seq:gtg)
     gene            362..433
                     /locus_tag="ACG8K3_002464"
     tRNA            362..433
                     /locus_tag="ACG8K3_002464"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Gln,seq:ttg)
     gene            451..535
                     /locus_tag="ACG8K3_002465"
     tRNA            451..535
                     /locus_tag="ACG8K3_002465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:caa)
     gene            790..1665
                     /locus_tag="ACG8K3_002466"
     CDS             790..1665
                     /locus_tag="ACG8K3_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9146280.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1769..2533
                     /locus_tag="ACG8K3_002467"
     CDS             1769..2533
                     /locus_tag="ACG8K3_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP9146281.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2520..3962
                     /locus_tag="ACG8K3_002468"
     CDS             2520..3962
                     /locus_tag="ACG8K3_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9146282.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3973..5304
                     /locus_tag="ACG8K3_002469"
     CDS             3973..5304
                     /locus_tag="ACG8K3_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP9146283.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            5317..5775
                     /locus_tag="ACG8K3_002470"
     CDS             5317..5775
                     /locus_tag="ACG8K3_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9146284.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1967 a          903 c         1264 g         1834 t
ORIGIN      
        1 ctataattta ttctttattt tatagattga taatagtata atggcggtat agccaagtgg
       61 taaggcagag gtctgcaaaa ccttcatcac cggttcaaat ccggttaccg ccttagtact
      121 aataaatcaa cttgccggcg tggcggaatt ggcagacgcg ctggactcaa aatccagtgc
      181 ccgttgaggg cgtgccggtt cgaccccggc cgccggtatt gactaagatg tcttcatctt
      241 agttttttat tgagaaatta aataacatct tttatgatgg cgaatgtggc gaagtggtta
      301 acgcaccgga ttgtggctcc ggcattcgtg ggttcgattc ccatcattcg ccctttattc
      361 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      421 ccagctaccc cagttattat ttttaaattt gccggcgtgg cggaattggc agacgcgctg
      481 gactcaaaat ccagtgtccg ttgaggacgt gccggttcga ccccggccgc cggtataatt
      541 atcaatatag caactttctt atagagagtt gctatatttt ttttgttttt atgtaattac
      601 ctccaaatca attcgataca agtattatca taaaacgagt aaaaaagata ttgataacgc
      661 ttttattatg tgttaaacta tttttgtaaa ttatgtaggg tgctactatt caattaggca
      721 cgacctaaga aagggttctt tcttaggtcg ttttttgtct ttaaaaattg tttggagaca
      781 aaaatatgta tgaaggagtc tgcaagttgt tttgttttat catatagagg aggtgaatta
      841 acggtgtatg tgatgaaaaa gctgaatcat aatgctgtat tgatcgatga cgatggaata
      901 gaaaaaattg cgatggggaa agggattggc ttttctgcta aagtgagtaa gccatttgat
      961 gttgaggatg ctgacaaatt atttgtatta gattctaaag aaaagatcaa acgattcagt
     1021 gagatagtct cgcagattcc aatcgaattt atggattttg cggaagaagt gattgaactg
     1081 ataacagcta ggatctcctc tccattagat tcaaatatct atattacatt gacagatcat
     1141 atttattttg ctattcaacg aaaaagagaa gatagagatg tcacagcaat tatgttgcca
     1201 gaaatgagat tcctttatcc aaatgaatat gatatagcag tagaagtggt cgagcgaatc
     1261 aatcatcgat atcaaaccga gttagggtca aatgaagtag gattcatcac tatgcatatc
     1321 attaatgctg agctaggaga gcctaatagt ttaaagagct taaaaatagt agaaatgacg
     1381 accacgattt tgtcccatct agaaaaatcc tattcaaacg tttatgataa agaatcactg
     1441 acttatcatc gcttaatggt tcatatcaaa ttcctggtta aacgcttgat ttatcaggaa
     1501 gaaacgccag tagaaaatat tggttttttt actgaaacat ttaaacatag caaagcgtat
     1561 tgtacagcac taaagattaa agatatcatc gaacagcaat ttcaacttgc cattcaagaa
     1621 aatgaaatca tctatttggc aattcatcta gcaagaatct attaaaataa gggcgtaact
     1681 atctataggc gcaaccaaat gacacatagt aaatggacta tgattatttg gttgcgccta
     1741 ttttagtaga aaaaagaagg agaatgaaat gggaaaagtg atttttaatt cggatgattt
     1801 tggctatagc catggtgtga attatgggat tatggatgct tatcagcgag gaattttgac
     1861 atcaactaca ttgatggcaa atatgccggg tttcgagcat gctgtgaaac taagaaagga
     1921 aatgcctagg ctaggtgtag gagtccattt aacattgacg tgtggcaaac cactcctcaa
     1981 aactgtcgat actcttatcg aaagcggaga gttcagatcc ttatcttatt atcagcatcc
     2041 atttaacatt gacaaagatc aactgtatca ggaatggaat gctcagattc aaaaaattta
     2101 tcatgcgggg atcataccaa ctcatttaga cagtcatcat catactcata ctttggatga
     2161 aaatcaggag gtagttgtta cattagcaaa aaaatatgat ttgccggttc ggagcaactt
     2221 tgaacgaaaa gatgaagttc gtcatgtcga ttattttgag ccttgtttcg atgtcgtagg
     2281 agaaactgat gaaaaaaaac gacaaatcaa tacgagtgtg gaagaatatt tggcaagttt
     2341 actcatgata ttgagagaag ataaaacgat cgaagtcatg tgtcacgcag cgtatttgga
     2401 tcaggaattg ttaaatggtt caagttttgt gtatcctcgt acccaccaag tcgaattttt
     2461 aattcattca gcatttgctc aaaaagtgaa aaatgatcct ttgattgagt tagtaacata
     2521 tgaggatatt taatagagga ggaataaaaa tgaatgttaa atcaaatgtg cagaagcttg
     2581 gaagagcgat gctacttcca gttgcagcga tgccattagc gggattgatc atgcgtttat
     2641 cagctgacga tatgctgaac atcccagtaa ttggtgcagc tggaaatgcc gtttttggaa
     2701 acttggatat cttatttgct ataggagttg caatcggttt tgtcaaaaat aaggacaaag
     2761 gaatacctgc acttactggt tttttagcga ttgctacctt gaaaaaaggc ttagaaatta
     2821 tggacccaga agtgaatatg gggatttttg gtggaattat ttccggttta gtagcggcat
     2881 ggacgtataa tcgctttaaa gaacaaaaat taccagtagt tttttcttat tttgcgggcg
     2941 aaaaattccc attgactatg gtgatgattc tacaaacaat cgcagctgtt tttttgggct
     3001 ttatctggcc gctcatccaa attgggattg ataattttgc aaaactgctc gttgatatgg
     3061 gagcattagg tgtggggata tttatgtttt tgaatcgttt gctgatcccc tttggactgc
     3121 accatgtgtt gaatacgtat atttattacg atttaggaag ctatacatct cctagcaagg
     3181 aagttttcag aggagagatg acacgcttta tcaacggaga tcctcaggct ggactgtttt
     3241 tatcaggttt ttttgtagta atgatgtttg gtataccagc tatatgtcta gctatctata
     3301 aagcagctta taaagaaaat aaagagatgg tcaaaggaat catgggaagc ggtgcaggaa
     3361 cttcatttat tgcaaatatc actgagcctg ttgaatttag tttcatgttt ctttctccta
     3421 tgttgtacgt gatccatgca ctttttgccg gtttagcagg gattgtctgt tacttattca
     3481 atatacgaat tggttttaca ttcggagcat gtattgttga ttatctcatc aattttcgaa
     3541 tcgctaccaa tgctattttg attcttccaa tcggaatttt cttttttgcc ttgtattacg
     3601 ttacttttta ttatttgatc aataagagaa acattcagac attaggacgt gaagctaagg
     3661 cagagtttgg caatgaagta accttagaag aaacagagtt agggttagca agtaaaaatt
     3721 atgaatatat ggcaacaaaa atgctacaag cgtttggagg aaaagccaat atcctggatg
     3781 tctatagttg taatacacgc ctacgggtag aggtagtgga tcccaccatg gtcgaggaac
     3841 agcgaatcaa gcaattaggg ataagcggga tcattaaacc aactgaaaaa aattatcaaa
     3901 ttatcatcgg cttagaagtg acttatgtta tggcagaatt caataaatta ttagaagaat
     3961 agaaaggaaa agatgatgat gaaaaaacag attatcacta tagctggagg cgggagtacg
     4021 tatacaccag gaatcgttca agccattttg aacaatgaaa aacggttgcc attatctgaa
     4081 atacgtttat atgatattga tgaagaaaga aatatggata tgtacttgat tgttaaattt
     4141 atgctgaaaa gaaaaggttt ttcaaatatt cgcattcgtg caacggatga tccaaagctt
     4201 gcttttacag gatgcgattt tgttttctca caaattcgtg ttggaggatt ggagatgcga
     4261 gaaaaggacg aaaagattcc acttaaacat ggattagtag gacaagaaac gtgtggactc
     4321 ggaggatttg cttatgggat gcgctcaatc aaaggtttat tggaaattgt cgatcatata
     4381 caagactatg cccccaaagc atggattttg aattatacaa atccagagtc gatcgtttct
     4441 gaagctgttc gtcgtaaata tccgaatgta aaaatgatta atgcctgtga tatgactatt
     4501 tcaattgaag aaacgattgc agtgaattac ggttatgatc gtaagaattg gatcgttacg
     4561 tattatggtt taaaccactt tggctggtat acatcgattt atgataaaga attgcaacga
     4621 gaaattatgc cagagattat tgaaaaacta ataacaaaag aaatgcaagt cgccgatttt
     4681 aatataggag ataaaacttg gcaaaaaacc tttcaaatga tgtcagtcat cacgaaaaat
     4741 tttccaagta atatcccgaa taattactta gagtattact tatatcctga tatggtcgtt
     4801 gagcatactg acaaagagta tacacgtgct aatatggtca tggatggacg agagaaaaat
     4861 accaaagaaa tggctgataa aatacgccgc ggtattgtgg aagaagtatt gaactttaat
     4921 tttggggagc atggacaata tatcgtcgat atagctattt ccttgttgaa tgatgatcgt
     4981 cgtcgcttta tgctaatcgt tcctaatcaa ggggcgattc cgaatctacg gtcagatgca
     5041 gttgttgaaa taccggcata tgttggtgca actggtgtgg agccaattac actaagaaaa
     5101 ccaatcagtg attttcataa aggattgatg gaggcacaag ttgcagtaga gaagttacta
     5161 gtagatgctt actttgaagg ttcttatcaa aaagctttac aagcatttac attgaatcaa
     5221 accgtaccaa atgccagagt cgcaaaaaaa atactagatg aaatgatcaa agtaaacaaa
     5281 aaatattggc cagcattaaa gtagaggtga taaaaagtgc atttctttaa aacaagaaac
     5341 attctaaaag ctgtagctag tggtcaaatt attccattaa cagcagtaaa agacgaggtc
     5401 ttttcttcaa aaatgatagg agttggatac gctataacga atcatgatgg atatatccat
     5461 gcgccagtta aaggaataat cgataatata tttcctaccc gacatgccat aacgatcgaa
     5521 agtgaatctg gcagaaaaat actgatccat atgggtttag atacagttga actcgaagga
     5581 agtccgtttc agatacgggt tgaaagtaac caggaggtaa aagaagggac actgttagct
     5641 attatagatt tgaagaagtt ggagcaagca caaaaagata cggtgatcat tgtagtttct
     5701 ccaggcattt ttgaaggaaa tttattaaaa caaaaccaag aagtaaagat taaagatagt
     5761 tgttttgaat tttagtgtac gatgaaaaaa gttaaatagg gtgatattta tgggtgaatt
     5821 tacaaaattt ttttgaaatg atatgtttca atcactagat ttaatcattt tgatttttgt
     5881 taattatcga atttttttgt tgctcctatg atttagaaaa cttattttca ggattcttaa
     5941 aaataaattt gattatttaa gagaatat
//
LOCUS       DAZKCN010000127         5740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000127 DAZKCN010000000
VERSION     DAZKCN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.131"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..620)
                     /locus_tag="ACG8K3_002471"
                     /pseudo
     CDS             complement(1..620)
                     /locus_tag="ACG8K3_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            768..1936
                     /locus_tag="ACG8K3_002472"
                     /pseudo
     CDS             768..1936
                     /locus_tag="ACG8K3_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2062..3141)
                     /locus_tag="ACG8K3_002473"
     CDS             complement(2062..3141)
                     /locus_tag="ACG8K3_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9146285.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3244..3684)
                     /locus_tag="ACG8K3_002474"
     CDS             complement(3244..3684)
                     /locus_tag="ACG8K3_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9146286.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3741..4151)
                     /locus_tag="ACG8K3_002475"
     CDS             complement(3741..4151)
                     /locus_tag="ACG8K3_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP9146287.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4331..4765)
                     /locus_tag="ACG8K3_002476"
     CDS             complement(4331..4765)
                     /locus_tag="ACG8K3_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9146288.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4865..5713)
                     /locus_tag="ACG8K3_002477"
     CDS             complement(4865..5713)
                     /locus_tag="ACG8K3_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP9146289.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2087 a         1071 c          957 g         1625 t
ORIGIN      
        1 agtacttctt ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca
       61 taaaattctg tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga
      121 atttgtacct cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa
      181 ccgtactatg ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta
      241 tagtttgttt gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc
      301 aagatctgat aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa
      361 gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt
      421 tctgttagat tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt
      481 gattcagaca tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca
      541 gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta
      601 atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa
      661 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
      721 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
      781 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
      841 acacagagga gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
      901 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
      961 aaacagcgac catcaaagca aaaattgact attcaggaga cttagcacct atactacaat
     1021 cagctgttac atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt
     1081 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
     1141 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt
     1201 taacgccgta tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag
     1261 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
     1321 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
     1381 aaactcaacc agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag
     1441 atgtagaccc agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc
     1501 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
     1561 aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc
     1621 aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta
     1681 aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa
     1741 tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg
     1801 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
     1861 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
     1921 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
     1981 tatccagaaa atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
     2041 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
     2101 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
     2161 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
     2221 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
     2281 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
     2341 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
     2401 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
     2461 caacgtattt aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac
     2521 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
     2581 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
     2641 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
     2701 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
     2761 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat
     2821 gtcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
     2881 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
     2941 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
     3001 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
     3061 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
     3121 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa
     3181 aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca
     3241 gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg
     3301 cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc
     3361 ttctgcttgt ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc
     3421 atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg
     3481 agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt
     3541 cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag
     3601 gaggggagtg tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg
     3661 attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata
     3721 gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca
     3781 gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct
     3841 tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca
     3901 ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc
     3961 tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt
     4021 tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta
     4081 cgatcgggat tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac
     4141 atttttttca taaaattacc acctttaatt atattttata caattgaatt ttacacaata
     4201 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
     4261 agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc
     4321 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac
     4381 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
     4441 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
     4501 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
     4561 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac
     4621 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
     4681 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
     4741 aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
     4801 agattctttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac
     4861 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
     4921 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
     4981 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
     5041 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
     5101 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
     5161 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
     5221 taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
     5281 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
     5341 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
     5401 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc
     5461 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
     5521 taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta
     5581 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
     5641 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
     5701 tcgtttcttt catttattca cttccttaat tcattaataa
//
LOCUS       DAZKCN010000128         5727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000128 DAZKCN010000000
VERSION     DAZKCN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.132"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8K3_002478"
     CDS             196..921
                     /locus_tag="ACG8K3_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146290.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8K3_002479"
     CDS             911..1420
                     /locus_tag="ACG8K3_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146291.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8K3_002480"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8K3_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9146292.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8K3_002481"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8K3_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9146293.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8K3_002482"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8K3_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9146294.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8K3_002483"
     CDS             4129..4902
                     /locus_tag="ACG8K3_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP9146295.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8K3_002484"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8K3_002484"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZKCN010000129         5604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000129 DAZKCN010000000
VERSION     DAZKCN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.133"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..2220
                     /locus_tag="ACG8K3_002485"
     CDS             160..2220
                     /locus_tag="ACG8K3_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP9146296.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSNVYEKDYSQFEGKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2303..2872
                     /locus_tag="ACG8K3_002486"
     CDS             2303..2872
                     /locus_tag="ACG8K3_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146297.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3047..3853)
                     /locus_tag="ACG8K3_002487"
     CDS             complement(3047..3853)
                     /locus_tag="ACG8K3_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146298.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3991..5505
                     /locus_tag="ACG8K3_002488"
     CDS             3991..5505
                     /locus_tag="ACG8K3_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP9146299.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1995 a          968 c         1132 g         1509 t
ORIGIN      
        1 agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc
       61 ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat gaaaattact
      121 acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca
      181 aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac
      241 tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat
      301 gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc
      361 actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt
      421 cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta
      481 attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt
      541 tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat
      601 catgctttga cagatataaa ggacggtgga agcgcacaag tttctaatgt atatgaaaaa
      661 gattattcac agtttgaagg aaagaaaatc gatgttctgg cagggacaaa agtttcgata
      721 tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta
      781 atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg
      841 tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt
      901 gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc
      961 cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa
     1021 atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt tgtattaact
     1081 tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca
     1141 tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt
     1201 caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa
     1261 ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact
     1321 tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc
     1381 cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc
     1441 tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat
     1501 ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa
     1561 cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat
     1621 ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc
     1681 caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa
     1741 aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca
     1801 gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga
     1861 aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct
     1921 ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt
     1981 gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc
     2041 ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg
     2101 ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt
     2161 atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga
     2221 tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat
     2281 ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat
     2341 gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg
     2401 attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt
     2461 tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga
     2521 acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac
     2581 cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa
     2641 accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt
     2701 tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc
     2761 gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc
     2821 taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg
     2881 tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt
     2941 tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag
     3001 tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt
     3061 ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt
     3121 aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat
     3181 ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg
     3241 cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt
     3301 ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt
     3361 gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa
     3421 aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat
     3481 tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta
     3541 gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg
     3601 aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata
     3661 tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat
     3721 ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata
     3781 aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt
     3841 tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg
     3901 tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta
     3961 atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca
     4021 gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca
     4081 agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca
     4141 gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg
     4201 atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg
     4261 cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa
     4321 tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa
     4381 ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat
     4441 gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg
     4501 atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg
     4561 gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg
     4621 acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa
     4681 gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc
     4741 aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa
     4801 ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg
     4861 gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt
     4921 gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg
     4981 gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg
     5041 atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga
     5101 tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa
     5161 acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa
     5221 ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa
     5281 atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta
     5341 ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc
     5401 ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga
     5461 caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata
     5521 aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg
     5581 tttagtgaag aggttgtgaa aaag
//
LOCUS       DAZKCN010000130         5315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000130 DAZKCN010000000
VERSION     DAZKCN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.134"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    complement(32..259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(621..1823)
                     /locus_tag="ACG8K3_002489"
     CDS             complement(621..1823)
                     /locus_tag="ACG8K3_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9146300.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(1883..2086)
                     /locus_tag="ACG8K3_002490"
     CDS             complement(1883..2086)
                     /locus_tag="ACG8K3_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP9146301.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(2228..3295)
                     /locus_tag="ACG8K3_002491"
     CDS             complement(2228..3295)
                     /locus_tag="ACG8K3_002491"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP9146302.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(3353..3460)
                     /locus_tag="ACG8K3_002492"
     CDS             complement(3353..3460)
                     /locus_tag="ACG8K3_002492"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146303.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            3623..4435
                     /locus_tag="ACG8K3_002493"
     CDS             3623..4435
                     /locus_tag="ACG8K3_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146304.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(4809..5000)
                     /locus_tag="ACG8K3_002494"
     CDS             complement(4809..5000)
                     /locus_tag="ACG8K3_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146305.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1647 a         1083 c          821 g         1764 t
ORIGIN      
        1 aaaaaaaaat ccttttacta gcaaatacta gtaaaaggac gattatactc gtggtaccac
       61 cttttttcag aaaaaacatt ctttctctca gcctgacaat aaaatcagtg gtcttggtaa
      121 cgaaagactg ttcgtttttg cctacctttc gacaaaaaat cctctaaggc cattttcgac
      181 agtcaacttc tagcttttta caccaaccaa agcctctctt gaaccatttt ctgtctactt
      241 tccttctcat cggatactat tagaataaaa ttagaatagc atgtttgaaa gaaaagtcaa
      301 tagggaataa gaacaaacaa aaactaattt gttattctta gtaatttgtt tgtggaatac
      361 aataaagctt ttctaatatg ataattgttc agtcgtcctt tcatcttaga tcacttcctt
      421 aaaatgatca tacccgtttc aaccacataa aaaaggactt aattttgaaa aatttcccat
      481 caaaactaag tcctttttat tatagttatg caaatttgct tattttctcg ctatttccga
      541 tacatcttga acaggtgcaa ttcgactgaa aacactggtg caaaagcatt tatttgtctt
      601 atggtagaac cgtggaaaac tcagactggt aataaattgt cattttttga aagaatagcg
      661 ccctcttgaa cattatcaat cgcacttacc acttgttgat ctgactttgc atataagtga
      721 ccgtaagttc ctaaagtgat ttgaatattc gcatggccca atcgttcttg aatagatagc
      781 ggttgaatat tctgctcaat tagaaatgca acatgagaat gtcgtagcgc gtgaagctta
      841 atcggtttga cacccgcttt tttagcagct tcttttaacc agttcgcaaa agaataaggt
      901 ggcgtaaatg ttccgtccaa ctgagcaaca tattcaaaat ttccaatacg tttttgcaag
      961 tcataccact gtctgagttt taaggaaagc ttcttcccca acccaatcgt tcgataagca
     1021 catttagttt tgggagtcga aaagtaccaa tcatcttttg tattataaat caatgtcttc
     1081 tcaatcgtta tcgtgttttt ctcaaaatct acattcgacc aagacaaagc ttcaagttct
     1141 ccaatccgaa tgccagtata aaagagcatt tcatacacaa tcttccgatg gtgggtttga
     1201 atcttatcat ccttcaattc attcatcact ttttgaaact cactgtgttc ccaaaactca
     1261 actttcttat tctcagaagg aagcgaagaa acatcttcaa cgggattttt agcaatcacc
     1321 ccctctttaa cagcccgatc caatacaatt tttaggtgcc ctcgaatccg attgatataa
     1381 gattttgata acgtttcgcc attaactttt ttccgcgaat tacgtgactc tgtctggctt
     1441 agttcaaata accaatcctc aatatcgtga cttgtaatat ctcgaacacg aaaatgatag
     1501 aaaaactgca tacgcttttc gaaaatgaat tgcgcattct catatgtttg acttttgacc
     1561 tgtctctcgt accattttac aaacctatcc ttgtaaaact cttcgaaagt catttgattg
     1621 gtagaacaca gttgattagg aattgtcttg ttaaaatcat gtgtttccat gtacttgtca
     1681 taagcaattt tggcatcttt ttgtgaagca taacctcgtt tagtcttctg aacctttctt
     1741 ccattttcat ctatacctag atacgctcgg aagaaccatt tttttgtctt tttatcttgg
     1801 taaatatttt taatttttgg catcgttcca ttcctccctg tacttgagaa aagctacttt
     1861 aatagtaccg cgcttgtact gtttattccg caacttgaat gtgaaaaaga tgttcaatta
     1921 ctcgggatgg caccacacca acttggcgat tattataaaa atcaatacct tcaatccttg
     1981 caagatatgc cttgctctct ttaatcattc gtgcagcttg actaggttta aagcccatct
     2041 ctatcaaaat tgcacgattt accatgaaat cacattttgt ttccattctc tcacctcctg
     2101 attctttact ccttttaaaa taatcctctt tataatctat tgtttctcat tcgatggttg
     2161 tgttatactc gtcttgtcga ggcggagtac gttgcacaac ttgttgtgta agcagtactt
     2221 tgctttttta gtagcttgtt ttctcatttg atatactttg taattttcgt tcataatact
     2281 caacctcctc ctgataatac tccgggtctg ccttcatatt ttggagaagt ttgatctgtc
     2341 ttggattcat ttctccttgt tcaataatca aaggcaacaa ggaagaatga aacgtttgat
     2401 cgacctgtgc aataaaaact aatgatggtg aaacaccatg aattaaccaa ctcatagttt
     2461 tctcaaaact ttgaggaatt gtatctaaag aaatggtgat tgcgccataa ttgctaataa
     2521 aggctgccca tttggcatca acttttgcac ctagatcatt tggatcacgg tcataaaaac
     2581 aaattgctcc attaagtaac tcaaagaaca gtttggttaa atcatgggtt tttgaaataa
     2641 tctttgccaa gctttgcgct ttctcttttc gataccgtag ttcaaagcga ttctttactt
     2701 ttgcctcttc taaaggaatt cctcgtcgct tccgttgttc atggtccttc tgataaaagc
     2761 aaaaacgaca ctgactcttc tttgagccta aatacaaggt ctcaccattt gattcattgt
     2821 ttatgatatc cgtaccgcca tgagattcac tcacttgaaa agtggtccac acatggccta
     2881 gtattacctt atgcttaagt tctggaatgc tgagactccc cacaaaatca tctaaagtaa
     2941 aatcaatccg tgtaaagttc ccctgataat ctaatacctt acgaaaaaac tgttgccaat
     3001 ttgtttttcg tgtttttagc catcttgcta agtgtttaca gccttgacct gaaaactcca
     3061 tcacagttcc tttaaccgca tcgtcaatgg aataccgaat cgtgattact tgattccaca
     3121 cgtattggcc aatatagccc tttggcgcat aatccagttc ctcaaaacgt tcaatgggca
     3181 tttcaataat ctcttcaatt agctttaaat gttgattcga gaaaaagtga atggttagat
     3241 aatcaatcaa aacacggtta actggaacga cctcttcttg caaactatcc aacacaagat
     3301 tcaatttttc ttcacttaat tgtctcgttc ctttttctac tttcgataat aactatctcg
     3361 aaatccccac tttttctgct aaatatcgtt gtgtccaccc cctttcttct cgagaaactc
     3421 gaatggcttc accgagatca tgttgattaa cttggttcat agacaaacct ccttctgcta
     3481 tttacttgtc aaagaaccta ttgaacttat tttaagtaca tttttattgt acgcctgtat
     3541 ttattttaag tcaatatgtt ttgacttttt ttaagtacac acgtatacta aatttaagaa
     3601 cttatcctga ggaggagttt tcatggagat agacaagcaa gcagtcggca atcgaattcg
     3661 gcagattcgc caagaattaa agttgagtat ggagaaattt ggcaaactaa ttggtgattt
     3721 accgcgaagt acagtcaata actgggaacg tgggatcaac cttccaaaaa ccgaaaccct
     3781 tcaccaaatc gcagagatag gtcatgtcac caatgaatat cttttgtatg gtgatcaaga
     3841 gaatcaatac attttagaaa tgctccaaaa aaaggcaggt aagcttgatc ctaagattga
     3901 aggtctcata gtagacgaaa tcaaacacgc tgaccttgtt agtgaaaaag aaatggatcg
     3961 aatgattgaa ttttttatta caaatctcat tccaccaact gaacaagacc agtttacttt
     4021 ccaatgtatc gatgaaaaaa agcagcttta tcttggttca actaattttg gtaaggaagc
     4081 ccagctctat ttgcaacatg ataatcaaaa tcatattctg cacatgatgc cctttacctt
     4141 ctccaacttt agtgtggatc gcatactcgt ctatttagca aatcaaaaat cctattccta
     4201 ttttgggaaa catttaccca aggaactagg tgagaaagcc atccttctat actcaattaa
     4261 tcaattgacc agcgatgtgc gaattgcccc gttggtctat tcagaagaaa ccgcttctta
     4321 tcagtacaca gaggacaatc aatatctact agaacagcaa tatctttatc taccattcgt
     4381 gatggaagtc gagaaagaac gcctgttaaa tgcagcgtat ccatcatcaa aatgaggtga
     4441 caaaattgat aagcacgtga caaagaaaat actgctagga cactccccaa tgggtagttg
     4501 tcacagtttc ctgttctttg ttagccccct gttagaaccg ggggctttat tcctgcagtc
     4561 cggctacgcc ttcctgcaat agacaaaact ccagatcggg taattcgctt gtggcgaaac
     4621 ccgacctgga gtttgtctct gtattttgaa ccctaaaaat taatcaaaca gactgatatt
     4681 ctcataatca gcgcgaaata agccattaag tacagttttc ctacctattt ttgataaccc
     4741 gctggttagt taatattcct tttccgagag agataaatga ttggttaaat ctatatacat
     4801 ctcaaccttt aaaaatgttt tcgaaatact tcatcagtac atgtcataca aattaccagt
     4861 gcattctcaa attctagttc tttaattgat ttcagttgag gagcagttac ttttggcgga
     4921 aatttccatt tttgtccaaa cctcttcccc cgatgaaatt tctttgttgc aattattgca
     4981 atgaaaaatt tttgtttcaa aaatatacat aattacttcc tccaataaaa actatctacc
     5041 tctcaaatta tttttctcta ataagaaaaa cagaaaaggt aataagatac aacagtgatg
     5101 attcctaata aagaaaagac tacaataata tttttctaac ttatcgcctt caaagaaata
     5161 gtttcttatt tttcactaaa ataaaaaatt tattgcaaaa aaaatagaag tgacaaaatt
     5221 atcaatcatt tcaaggtaat ataactacct gagtttatta tcgattaaaa tgctaactac
     5281 atttgcaaga gaagcgagag ttaaaggtaa atata
//
LOCUS       DAZKCN010000131         5128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000131 DAZKCN010000000
VERSION     DAZKCN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.135"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8K3_002495"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8K3_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP9146306.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8K3_002496"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8K3_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9146307.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8K3_002497"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8K3_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP9146308.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8K3_002498"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8K3_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP9146309.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1533 a         1047 c          774 g         1774 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 actgtactat tattttatct caaaagctaa atgaaaccga tttcttaaga ttttttatgt
     4981 tttttatttc tttaatagta atgttttctg tcattttaaa taatactgtt ctttattata
     5041 acgcttattt tctctgttat ataagcgtag tattttaaag caatgattac taaataaaat
     5101 ttactataaa caaataaatc aaataacg
//
LOCUS       DAZKCN010000132         5093 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000132 DAZKCN010000000
VERSION     DAZKCN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.136"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..2000
                     /locus_tag="ACG8K3_002499"
     CDS             66..2000
                     /locus_tag="ACG8K3_002499"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9146310.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2457..2873
                     /locus_tag="ACG8K3_002500"
     CDS             2457..2873
                     /locus_tag="ACG8K3_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP9146311.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2887..3453
                     /locus_tag="ACG8K3_002501"
     CDS             2887..3453
                     /locus_tag="ACG8K3_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP9146312.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3476..4462
                     /locus_tag="ACG8K3_002502"
     CDS             3476..4462
                     /locus_tag="ACG8K3_002502"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP9146313.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4617..>5093)
                     /gene="scm"
                     /locus_tag="ACG8K3_002503"
     CDS             complement(4617..>5093)
                     /gene="scm"
                     /locus_tag="ACG8K3_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP9146314.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         1541 a          943 c         1122 g         1487 t
ORIGIN      
        1 agtatcaaac taaaagaatt atcatatatt cattatatag atagtctata aggagcggat
       61 gatcaatggc aaagcaaaat attgtcgttg taggagcagg atatgccggt gtttcagcta
      121 caaaatactt ggcaaagaaa ctgaaaaaag aagatgtagc aattactctg atcgatcgtc
      181 attcgtacca tactatgatg accgagttgc atgaagtagc tggcggacgt gtagaaccag
      241 aagcaatcca atacgatctg cagcatttgt ttgctcgtca aaagaatgta caacttgtca
      301 cagacactgt aatcggtatc gataaagaac gaaaaatcgt caagaccaaa ttaggttcct
      361 atccttttga tcagctgatc cttggaatgg gcggagagcc gaatgacttt ggtacacctg
      421 gtgtcaaagc acacggattc accctttggt catttgaaga ttctgtaagg attcgggaac
      481 atattgagaa gacagtagca aaagctgcat tggaaccaga tgatgaactg cgtaaagcca
      541 tgctgacttt cgttgtctgt ggttctggat ttaccggtat tgaaatgatt ggggaactga
      601 tcgactggaa aaatcgccta gccaaagatt ataaattaga tccaaacgaa atcacactga
      661 tggtcgtaga ggcaatgccg acgatcttga atatgttgga tcgaaatgat gctgcaaaag
      721 ctgaacgcta tttgcgtaag cacaacgtgg aattattatt gaatgcacca atcgtagaag
      781 tggctgctga tcatatcaaa ttgaaagacg gacgcaacat tccgactcac acactgattt
      841 ggacagctgg tgtcaaagga acttctgacg ctgctgactt tggattagaa tcagcacgcg
      901 gacagcggtt gatcgccaat gaatatatgc aggcaaaagg ctacgaagat aaaaacatct
      961 atatcattgg tgatttagtc ttttatgaag aattcccagg aacaccgaca ccgcagatcg
     1021 ttcaagctgc tgaacaaaca ggacacacag ccgcagcgaa tatcgtggct tcgatcaaag
     1081 gcggagaaaa gcataaattt aaaggaaact atcaaggatt tatggtttct attggtgcca
     1141 aatggggggt agctaaccta tttgataaga tccatttaag cggattttta gctatcgtaa
     1201 tgaaacatat cgtgaatttg aaatacttct ttgatattcg ttctggttat tatatgttcc
     1261 aatatttaat gcatgagttt ttccatatca aagatgatcg caacgtgact cgaggacatt
     1321 cttctcgtta cggcaatgtc ttatggagtg tgccacttcg tattttctat ggtttagttt
     1381 ggttcgttga agcttccaaa aaaatcattg ggaatggtga agtgttcaaa ccaagtactt
     1441 ggtttggtga aggatcatgg ttcactggaa acagtgtgtt cccttggaca tgggaataca
     1501 atgtaacaga tgttgccagt ggtgcttctg ctgctggtga tgcaaccaca gctgccagcg
     1561 gagcaggggc aagtgccgga gaagcagcga ctcaagctgc acattttggt ttgagttatg
     1621 catacggtga agaaccaatg gctgtgattg agaagtcacc agactggttc acaaagatca
     1681 tgcaagtgat catgccaaac aatgatgtag caatgttctt ccaaaaattc atggtattct
     1741 ttgaaattgc cttggcatta gcattgattg ctggattatt cacatggcta tgcagtgcga
     1801 caacgattgt attggtgatc acgttctgct tatcaggcat gttttactgg gtaaacattt
     1861 ggttcatttt tgttgctttc gcattgatga acggttccgg acgtgcgatt gggttggatc
     1921 gctgggtaat tccatggcta caacgtacac taggtaattg gtggtacggt agaccaagtt
     1981 cccagtacgg caagaaataa atagataaag aacgttattt gaatgcaaga tttcaataga
     2041 aaggggctga gacaaaccgt cacagcctcc tttttatgta aaattaaagg aataagatgc
     2101 ccgagataag cctccatgag caagacacag gtgtcacaac ctctgttaca acctcttgtt
     2161 ataaacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaacttg
     2221 aaaagtaatt ttcgtaaatt ccttcttatt tcttgaagtt gcccacttct gtcacaacct
     2281 cttgttataa acggtgttcc acctgctgct tctcggtaat aagcccaaag aattagaaaa
     2341 catggaaagc tgttttctat tcttcgttct tattactcaa agcaagacac aggtgtcaca
     2401 acctcttgtt tttatgctaa aattgggtga gttaatctgg aaaaaggaga acaaaaatga
     2461 gccttaaaga atttgctgaa aaaagtctgc tcaaaccttg ggatgttttt attattttgc
     2521 ttttgactgt tctttctttt ttaccagtca tgatttttgg tcttcagcaa aacaccgcaa
     2581 cagcagaaaa agaagcggta ctgcgtgtgg atggcacaga aatcaaaaca ttccctttga
     2641 tggctggaga agagagcaac acatatactt acaaagatga acacggagat tataacttga
     2701 tcgagataga tggcgatcga gtacggataa aagaagcaga ttgcgatgac cagatctgcg
     2761 tacgtcgagg ctgggcaacg aaaaatggcg aaaccatcgt ctgtttgcca cataaattag
     2821 tgatcgaagt ccgttcaaca gatggaggtg acgaagacag tctgatttat tagaccttca
     2881 cacaccatga gtaaattaca aaaaatgatt tatatttcgc tacttgttgc acaaggagtg
     2941 atcattggtt tattggaaaa tatgatccct tatccttttt cttttgcgcc tggtgcaaaa
     3001 ctaggcttag ccaatctaat cacgatcgtt gccattttta ccatgaaaaa acaagacagc
     3061 ttcttactac tttggctacg actattcctg actactttgc taggcggaac gatttccaca
     3121 ttcctttata gtatgagtgg tgctttgctt agttattttg gaatgttatt ggtcaagcaa
     3181 ttaggaccaa aacgtgtcag tatcatcgga attagtgcgg ccggcggttt catgcataat
     3241 gtcggacagc ttttgatggc ttctttcatt gcacaatcat ggacagtcat gttgtacttg
     3301 ccagtccttt cagttttagg gattttatca gggattgcaa tcgggatcgc agcgaattat
     3361 cttttacgac atgtgcagac acttcaacga ttccaaaaag attatgagca gggaaccaaa
     3421 caaaaatgga gcccaatgtt taccaaaaaa taatcgtcac acaaggagtt tttttatgaa
     3481 gctacatcca atgtgggaaa actatcctgc tttaagtaaa gaattgacag aaacactaaa
     3541 acggatggaa gaaactgtcc gcctgaaaaa caaacctgtc gaacaagcca tcaaagaaac
     3601 gatccatgcc ggcggaaagc tgttgcgtcc tgcctatcag ctattgttct cacaatttgg
     3661 accagagcaa gatcgagaca aagccatcgc tttggcagct tctatcgaaa tgctccatac
     3721 ggccacactg atccatgatg atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa
     3781 tatccgtagt cagtttggta atgtcgtttc tgtctatgca ggagactatt tatttgtctg
     3841 ctgttttaaa ctattgtctg attactccac ttccttgaaa agtctccaat tgaattctcg
     3901 cagtatggaa aaagtattga atggcgaatt agggcagatg gatgatcgct ataattatga
     3961 actttctgta aaagaatatt tggaaaatat ttcaggtaag acagctgaac tgttccaatt
     4021 aagttgttct gttggcgctt ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg
     4081 agatatcgga ctttctatcg ggatggcctt tcagattatc gatgatattt tagattatac
     4141 aaaagagagc caggaaatcg gcaaacctgt actagaagac atgcgtcaag gggtctattc
     4201 tttaccgttg atctactctt tgcaaaaaaa caaaccaaag ctactacctt atttagagaa
     4261 gaaagcggca atgacagaag aagatgtcga cgccgtacgc aaaatcgttg aacatacagg
     4321 aggcgtggaa gaagctagga agttagcggc ctcttacaca gaaaaggctt tgaaggaaat
     4381 aaagaaatta cctgccacct ctcttcgaac aaaagagaat ctcttttccc ttacccaact
     4441 gattttggat cgcaaagact gaatagggaa aggttcgata cgtaaataaa tttctcagaa
     4501 ggaaatctac gtgttttttg acaaattgaa ttgtagttct tgtatttatg ataaaaagca
     4561 aaaataaaga gcaacgtccg ttagcgacgt tgctcttttt tatcttgaat atttttttac
     4621 cttttcgatt tcttataact cataaaagtg attcttaaac cgattacaag taatataacg
     4681 cctgcgagtg ttatccaatt attcgattga cttccagttt taggaagtgc tctttttgat
     4741 ttgtttccat tgtcattgct tgttttatta gttaagttaa agttcgtcct gttatccgaa
     4801 cttgtttttg agaaggagac acttgcatga cgtgtatcgc ttgtggtaga cgacgtgtct
     4861 gtactatcgc ttgaagttga tgaatctgag ctatcacttg tggtagagga tgtatctgta
     4921 ctgtcacttg aagctgacgt gtccgtgcta tcacttgtag tggtagatga atctgagcta
     4981 tcacttgtgg tagaggtagt atctgtactg tcacttgaag ctgacgtgtc cgtgctatca
     5041 cttgttgtgg tagatgtatc cgtactgtcg cttgaagctg acgtgtccgt gct
//
LOCUS       DAZKCN010000133         4995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000133 DAZKCN010000000
VERSION     DAZKCN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.137"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG8K3_002504"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG8K3_002504"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9146315.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8K3_002505"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8K3_002505"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP9146316.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG8K3_002506"
     CDS             2591..3376
                     /locus_tag="ACG8K3_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9146317.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG8K3_002507"
     CDS             3389..4240
                     /locus_tag="ACG8K3_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP9146318.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG8K3_002508"
     CDS             complement(4364..4816)
                     /locus_tag="ACG8K3_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146319.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1690 a          818 c         1039 g         1448 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttcc atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctga
//
LOCUS       DAZKCN010000134         4987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000134 DAZKCN010000000
VERSION     DAZKCN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.138"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8K3_002509"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACG8K3_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP9146320.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8K3_002510"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACG8K3_002510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9146321.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACG8K3_002511"
     CDS             complement(2169..2906)
                     /locus_tag="ACG8K3_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9146322.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACG8K3_002512"
     CDS             complement(2997..3524)
                     /locus_tag="ACG8K3_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146323.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8K3_002513"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACG8K3_002513"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9146324.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACG8K3_002514"
     CDS             complement(4401..4886)
                     /locus_tag="ACG8K3_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9146325.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1326 a         1115 c          853 g         1693 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgc
//
LOCUS       DAZKCN010000135         4978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000135 DAZKCN010000000
VERSION     DAZKCN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.139"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..1478
                     /locus_tag="ACG8K3_002515"
     CDS             204..1478
                     /locus_tag="ACG8K3_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9146326.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1532..2959
                     /locus_tag="ACG8K3_002516"
     CDS             1532..2959
                     /locus_tag="ACG8K3_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP9146327.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2972..3844
                     /locus_tag="ACG8K3_002517"
     CDS             2972..3844
                     /locus_tag="ACG8K3_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9146328.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3939..4934
                     /locus_tag="ACG8K3_002518"
     CDS             3939..4934
                     /locus_tag="ACG8K3_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146329.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1714 a          687 c          976 g         1601 t
ORIGIN      
        1 taaaaaatag tgccattaaa aaagaaaagc gacacaccaa taaaagtcat atcctgtgac
       61 attatatggc attatccaaa tagaataacc ggttgacatg taagcggatt tcttttataa
      121 ttttaggagt acaagtcctt ttctaaaaat gttaaccggt tatcatgaaa ggggatacaa
      181 atatatcgtt ggaggataaa aatatgtcaa aagttaacgt atttaaaaat caatcttatt
      241 tacaaagttc agctacatta ttactatttt ttgcttcttg gggtgtttgg tggtcatttt
      301 ttcaactttg gctaacatct gaatcaaatg gtttagggtt atctggcagt gctgtaggaa
      361 cagtattctc ggcaaattcg ttagttacct taattttgat gtttatttat ggaacattac
      421 aagataaatt gtatattaaa cgaaatttat taatttttgc ttctgtatta gcgacacttg
      481 ttggaccatt ttttatatgg atatatgggc cattgctaga taacaatttt aatttaggca
      541 ttattatggg agcgctattt ttgtcagctg gatatttagc ttctgtagga gtttttgaag
      601 ctgtgtcaga aaggtttagt cgtttatttg gctttgaata tggacaagca agggcgtggg
      661 gatcatttgg ttatgccttg gtagcgcttt tggcaggatt tttatttgta aaaaatcctc
      721 atttaaactt ttgggcggga tctttctttg gttctttact attgttaaat ttattatttt
      781 ggaaccctaa agttgaacgg gaagcaaatc aaaattttaa tcaagaacaa gctgaatcaa
      841 atagtattcc ttctttaaaa gaaatgtttg atctaatgaa actgcctcaa ttatggacga
      901 taatcatctt tattgttttt acatggacat tttatacggt attcgatcaa caaatgtttc
      961 cgggatttta tactggtttg ttttcaacat cagctaatgg tgaaaaaata tatgggacat
     1021 tgaatgctat tcaagtattt tgtgaagcgt taatgatggg aattgttcca atcattatga
     1081 gaaaattagg ggttcgaaat actttgttat taggtgtaac cattatgtgt gtacgaattg
     1141 gattgtgcgg gtttgcctcg acaccattat ctgtttcatg cataaaaatg ttgcatgctt
     1201 tagaagtacc attatttaca ttaccaatgt ttcgctattt tacacttcat tttgatacaa
     1261 agctatcagc aaccctctat atgataggat ttcagatagc tgctcaaatt gggcaagtga
     1321 ttttatcaac accattggga atattaagag acaacgttgg ctatcaacca acatttaaaa
     1381 ttatttctct tattgtatta ctagcaggca tatatgcatt ctttattctt aaacaagatg
     1441 atagagatgt tcaaggggat ccatttattc gaggataatg gaaaccaaca gaataaaaaa
     1501 caattttatc tagaaagtga gcgaatatat tatgacaaaa aaagctatag aattaacaaa
     1561 tacacgttat cgtttgggat atcatgtgtc tgcaccatca ggatggatta atgatccaaa
     1621 tggattttgc tattttgatg ggtattatca tgtgttttat caacatcatc catatagtgc
     1681 tgaatgggga ccaatgcact gggcacatgc tagaagtaag gatttagttc attgggaatc
     1741 tttaccactt gctttgactc ctggagatca agaagatgaa ggtggttgtt tttcaggaag
     1801 cgctattgaa aaaaatggtg tgttgtatct gttctataca ggacatcact attatggaga
     1861 caatgatcca gatcattttt ggcaaaatca aaacatggcg tatagtacgg atggcattca
     1921 ctttactaaa tatgaaaaaa atcctgtaat agcaaaagca ccagaagata acacacatca
     1981 tttcagagat ccaaaagtat ggaaacacga tgaaaaatat tacatggttt tgggtagtca
     2041 agaaaaagat ggattaggtc gtgctattgt ttatcagtcg ttagatttat taaattggga
     2101 ttacattgga ccaatttcta aagcgaagga agtacttaca gaaggattta tgtgggaatg
     2161 cccagacttt tttgaattaa atggaaaatc tattctttta ttatcccctc agggaattga
     2221 agcaaatgga aaagattatt taaatctata tgaaacaggc tactttatcg gtgattatga
     2281 ttatgagaca gcaaaattca ttagaggaaa tttttatgaa ttagataagg gacacgattt
     2341 ctatgcaaca caaacaactt tatctccaga tggtagacgt attgttatgg cctggatgga
     2401 tatgtgggaa agccctatgc ctgaaaaagt agacggatgg gcaggagcat taacgcttcc
     2461 tcgagaatta gagttaattg gagatcgttt gtatatgaaa cctgtgagag aattagaaca
     2521 acttcgagtt ggcagcggcg ttgaaacgac agttaatttg gcaggagaaa taataatcgc
     2581 tcaagatgct tcattcaaag aagctctaat tgatgtacta ttgactaaat caaaaaatca
     2641 agaaatcaaa ttttcattaa aaacaaaaga tgaagagtta atcaatattt tttattctaa
     2701 ggataaaaat gaatgtacct taactcgtaa cggcaaaaat gatccaaggt atggaaccat
     2761 tcagatgtgt gatagattat cacttcgagt gtttatcgat aaaagttcgg cagagatttt
     2821 tattaatgat ggcgagcttg tttttacaga gcgttattat acagaaaatc aaccaagtat
     2881 gcatttgagt gttagtgaag aagcttttgt ctcgagtaca atttatcatc taaaaaaaga
     2941 tgcaatttct tacgagtaag gaggttttat gatgttaatt ggtagcatag aagcaggtgg
     3001 gactaaattt gtatgtgccg tgggagacga ggattaccga gtacaagacc aaataatttg
     3061 tccaacgacc acccccaggg aaacattgaa aaaaactgtt gactatttta aacagtttga
     3121 tataaatgca ttaggtattg catcatttgg tccaatagaa attcgaaaaa attcaccaaa
     3181 atcaggatat attacgtcta caccaaaggt gggatggcga aatgttaatg tgttaggtta
     3241 tttacaagat gagctgaatg ttccaatgtc ttggacaacg gatgtgaacg gatcagctta
     3301 tggagagtac atcatgtcca ctttatcgaa tgaaaaaata aattcattag tttattacac
     3361 cgttggtact ggcgttggtg gtggagctgt tgttgacgga aaactattag gatcgtttgg
     3421 tcattcagaa atgggccata cgtttgtaaa acgccatcct gatgatattg attttaaagg
     3481 aatttgtcct tatcatggtg attgtttaga aggcttagtt gcgggaccaa cgtttgaagc
     3541 acgttttgga aaaaagggtg aagacgtttc tttaacggat cctatttggg atgtaatggc
     3601 ctattatcta gcacaagctg tgattcaaac aacattgatt atacgaccgg ataaaatagt
     3661 atttggtgga agcgtagtaa atgaaaaatt gttagaaaaa attcgtgtta atgttaataa
     3721 attattggat gattacgtgg aaataccacc gttaagtaaa tatattacgc gtcctttaat
     3781 taaaaataat ggatcggcaa cactaggaaa ctttgctttg ggcttgaatc aattacaaga
     3841 ataaaacata gaaaaaatat cgattatcag gtatagtagt tctatagagt tctttttttc
     3901 gatagttaat tattgcatta tttaggagag acaagagaat gcgcccaaaa ttagaagatg
     3961 ttgccaaaag agcaaatgta tcaaaaacaa ctgtttcacg tgtattaaac aatcgtggct
     4021 atttaagtca aaaaacgatt gataatgttt ataaggctat agaagaactc aattatcaac
     4081 cgaacgttgt agcgcgtcaa ttatttcaaa agaaaactaa cattgtcggg ttactatttc
     4141 ctacagtagc caatcctttt tttagtgaac tcgttgaggc attggaaaaa aagttatatg
     4201 aaattggtta taaggtatta ataggcaatt cgatgaataa taaagaaaag gaaacaaatt
     4261 atttaaatca gttattgtct gatcaagtag atggtttaat tgttggtaca cataaccaag
     4321 gaattcagga atataaatat cagaatttac cgattgttgc aattgatcga gtgatgaatg
     4381 aagatattcc agttgttgaa tccgataact acaatggtgg aaaacttgca accaagttat
     4441 taattgcaca aggtgcaaaa aatattattc atacaaacgg tccaattgac ttacagactc
     4501 cggctaatcg tcggagacta gcctatgaag atactatgaa agcatatcag ttaattccac
     4561 gaactgtgac attagatttc aatatttctt atgtaaagaa aaaacagatt tttttccaaa
     4621 tgtttgagga ttatcctaaa atagatggta tttttgcttc aaatgacatc gatgcggccc
     4681 taatattaca agtagcaaaa gaaaaggggt taaatgtacc agctgattta ttagtagtcg
     4741 gatatgatgg tactttaatg actcggagta tattaccaga tctgacaaca gtaatacaac
     4801 caattaatga tatagcagac acagctgttg ctattttgat gaaacgaata aataaagaag
     4861 aaacaaaaaa ggaatatatt ttacctgtca cattatggat ggggaagaca tcagtaaaga
     4921 aaaagaacaa ttaaatttat aaagactatc aatgataatt tcattattaa ttcgtgat
//
LOCUS       DAZKCN010000136         4973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000136 DAZKCN010000000
VERSION     DAZKCN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.140"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..1250)
                     /gene="nagA"
                     /locus_tag="ACG8K3_002519"
     CDS             complement(102..1250)
                     /gene="nagA"
                     /locus_tag="ACG8K3_002519"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9146330.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1326..2162)
                     /locus_tag="ACG8K3_002520"
     CDS             complement(1326..2162)
                     /locus_tag="ACG8K3_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146331.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2346..3563)
                     /locus_tag="ACG8K3_002521"
     CDS             complement(2346..3563)
                     /locus_tag="ACG8K3_002521"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP9146332.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3712..3873)
                     /locus_tag="ACG8K3_002522"
     CDS             complement(3712..3873)
                     /locus_tag="ACG8K3_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146333.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3946..4029
                     /locus_tag="ACG8K3_002523"
     tRNA            3946..4029
                     /locus_tag="ACG8K3_002523"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3980..3982,aa:Leu,seq:gag)
     gene            complement(4269..>4973)
                     /locus_tag="ACG8K3_002524"
     CDS             complement(4269..>4973)
                     /locus_tag="ACG8K3_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146334.1"
                     /translation="TPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMN
                     ILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMI
                     WSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVIL
                     MIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFG
                     LTVLGICTFIITVINR"
BASE COUNT         1526 a         1025 c          798 g         1624 t
ORIGIN      
        1 agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac
       61 caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc
      121 acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca
      181 tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc
      241 agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta
      301 attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt
      361 ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg
      421 gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt
      481 cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct
      541 aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg
      601 aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta
      661 gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct
      721 tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct
      781 tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct
      841 ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt
      901 gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac
      961 gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact
     1021 ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta
     1081 tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca
     1141 ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt
     1201 ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct
     1261 ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac
     1321 accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct
     1381 gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg
     1441 aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac
     1501 gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa
     1561 aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa
     1621 tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc
     1681 agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct
     1741 tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg
     1801 agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa
     1861 gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt
     1921 tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc
     1981 gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta
     2041 ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc
     2101 attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc
     2161 ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt
     2221 cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc
     2281 cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt
     2341 taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc
     2401 aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat
     2461 ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca
     2521 tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt
     2581 ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt
     2641 tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg
     2701 gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc
     2761 agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact
     2821 cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca
     2881 ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt
     2941 tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat
     3001 tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc
     3061 cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg
     3121 caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag
     3181 tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg
     3241 atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt
     3301 ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag
     3361 tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca
     3421 aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc
     3481 atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg
     3541 ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt
     3601 tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac
     3661 ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc
     3721 atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag
     3781 cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc
     3841 cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca tttttgagct
     3901 tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa
     3961 ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca
     4021 tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt
     4081 tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta
     4141 aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg
     4201 ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt
     4261 tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat
     4321 ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc
     4381 attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc
     4441 catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata
     4501 actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat
     4561 agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt
     4621 ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat cattgccctt
     4681 ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg
     4741 tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat
     4801 gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac
     4861 ggctttccaa atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat
     4921 tcaggaaacc atttattaat ttcatgtgca gcacgtaatg cattccctgg agt
//
LOCUS       DAZKCN010000137         4930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000137 DAZKCN010000000
VERSION     DAZKCN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.141"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8K3_002525"
     CDS             85..972
                     /locus_tag="ACG8K3_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP9146335.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8K3_002526"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8K3_002526"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9146336.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8K3_002527"
     CDS             2274..2720
                     /locus_tag="ACG8K3_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP9146337.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8K3_002528"
     CDS             2993..4345
                     /locus_tag="ACG8K3_002528"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP9146338.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8K3_002529"
     CDS             4516..4803
                     /locus_tag="ACG8K3_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP9146339.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1392 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt
//
LOCUS       DAZKCN010000138         4849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000138 DAZKCN010000000
VERSION     DAZKCN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.142"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG8K3_002530"
     CDS             complement(220..972)
                     /locus_tag="ACG8K3_002530"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9146340.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2029
                     /locus_tag="ACG8K3_002531"
     CDS             1085..2029
                     /locus_tag="ACG8K3_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9146341.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQLCTRLARLY"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG8K3_002532"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG8K3_002532"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9146342.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAAFAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG8K3_002533"
     CDS             3311..4789
                     /locus_tag="ACG8K3_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146343.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1700 a          780 c          933 g         1436 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcatcca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgtacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttt gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat gacgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaag
//
LOCUS       DAZKCN010000139         4806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000139 DAZKCN010000000
VERSION     DAZKCN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.143"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACG8K3_002534"
     CDS             <1..411
                     /locus_tag="ACG8K3_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146344.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002535"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFP9146345.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /gene="mobV"
                     /locus_tag="ACG8K3_002536"
     CDS             2446..3708
                     /gene="mobV"
                     /locus_tag="ACG8K3_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFP9146346.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4806
                     /locus_tag="ACG8K3_002537"
     CDS             3953..>4806
                     /locus_tag="ACG8K3_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP9146347.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          981 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaaa
//
LOCUS       DAZKCN010000140         4677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000140 DAZKCN010000000
VERSION     DAZKCN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.144"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..2378
                     /locus_tag="ACG8K3_002538"
     CDS             6..2378
                     /locus_tag="ACG8K3_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9146348.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKLSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVQQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3899
                     /locus_tag="ACG8K3_002539"
     CDS             2439..3899
                     /locus_tag="ACG8K3_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9146349.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAILFQQGRQQERE"
     gene            3910..>4677
                     /locus_tag="ACG8K3_002540"
     CDS             3910..>4677
                     /locus_tag="ACG8K3_002540"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFP9146350.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKTTRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTYKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGISGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAQVTAQNGTFDAKLDPNETQVTQEAFEA"
BASE COUNT         1785 a          778 c         1011 g         1103 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaatg aacaaatcga aaagatgttt gaacaccaaa
      121 aaatgacgaa cggtcaacca atgaacgaca agaggaatgg ccgttcatgg tttaaccagc
      181 aagaacgaat cgaaaataaa gtccaaagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggttataac aagtacggcg
      301 gactagatat gacgatcgaa aatattccaa gaatcagaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gtctttttca gccgccacga tccgaaaata tcaacggaag ttggaaacat
      421 tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa tatcctccct gaagtacaag
      481 cgatcattga cagcggacga gtaacgcaat ggaaaaaaaa tccgaccatt tattttctga
      541 aaggctatcg aaaagtcgct ttagaactga atgaaaacgg ggaatttgag gaatctaaaa
      601 aataccaagc cacatcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctctgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtggaga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gacaaccaat acgctgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 agaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggatatc aaaaacgaac ggcagaagag attaaacaag
     1081 aagttaaatc actttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtcaa agaacttttg aactttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gccgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaactc agttaaaacc tgaagactat ccgaaaatct
     1501 tcccgaaccg tgtctttgat tttcaattgg atcaaacagg gcagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatatcggga ttcaaattcg agatattacg gagagtgaaa
     1621 tctatcaaag agaattagga caagcacgtg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaac tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acgtcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 ctgcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgcagcagga gcataaaaat actgtagaaa cggcagaaca aaagaatatt gtgcctgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagta attttaaaag
     2221 gaaaaagtta tggaaaagag atccaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gagacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaagaa agactatctg
     2401 aaaaatggat agtctttttt cttttgggag gtttgaaaat gacagagaaa aaaatgagtc
     2461 tcattgatcg atgcaagcaa atggatattg tggattttgc acgaaacaat ggaatggctg
     2521 tagtcaataa aggacgtgac tatcgcttag aagaccatga ttcgtttgtt ttcgatcgaa
     2581 gaaaacaacg tttttattgg aatagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt tatagacaaa gagatccaag accctaagca acaatttaaa gcagccactg
     2701 attttattct aaagaatgaa gccaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccacttac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaattg ccagagtggc tgattgatta tgcagaaaaa gaaggattga
     2881 ttactgaact aaagccaaaa catgaacggc aaaatttttt agttcgagac aatcgtttag
     2941 atcatgcagt tgccttttta tggaaagatc cacagacaaa agagactgtg ggggcgagtt
     3001 atcaaggaac gtttattgat tatgaacgtt ttggtgagag aggaacatat aagcatatag
     3061 ataaaaattc gacagcgaat catggcttta acctaaagat cggtgaccct aagcagttaa
     3121 aattttttga gagtagtatt gatttactta gctacgctgc actgaatcgt gatcagttaa
     3181 acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt cattattttg
     3241 gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatcgatt gagatttgtg
     3301 tggataatga tcgtgcagga catattttct acgaaaagga acaattaatg ggcgcagtcg
     3361 atccatttac gaatcagaaa gtacattgtg aacgaggaat cgctaatgat tggcaagtgc
     3421 caaaagaata caaaatcatt tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag
     3481 ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa ctggtttccg
     3541 ctcacaaagt caatgcgtct tttgaccagc agctttctgt taacgattca atagaagcta
     3601 ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaaggttgt gaacgcgtgg
     3661 atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat gctcagattc
     3721 ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa catgaatttg
     3781 tacctgaagt gaaaaaggat tggaacgatc aattgaagca tgagatccag caacaagaaa
     3841 tcagaaagca aaaacgtgcc atattgtttc aacaaggcag acaacaagaa agggagtgaa
     3901 atcgtcatga tgaaaaccgt tattttagca gaaaaaccgt cacaagcgaa ggcttatgcc
     3961 gatagctttt ctaaaaccac ccgaaaagac gggtattttg agatccaaga tcgattgttt
     4021 tcgggagaaa ctgtgatcac gtatggtttt ggtcatttag ttgagttaga ttcgccagat
     4081 atgtacgatg aaaattggaa acaatggtca ttagagcatt tgcctatttt ccctacacat
     4141 tatcgctatc atgttcctaa agataagaaa aaacaattta acgtagtgaa acagcaactt
     4201 caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga actgatagct
     4261 tggacgatca ttcaacaagc aggtgcagac cagggaaaaa cctataaaag actttggatc
     4321 aactccttag aaaaagaagc gatttatcag ggattccagc agttacgaga cgctggagag
     4381 acctatccaa aatttgagga agctcaagca cgtcagatcg ctgattggtt gatcggcatg
     4441 aacggtagtc cactatatag tttactcttg cagcaaaaag gcatatcagg aagcttctcc
     4501 ttaggcagag ttcagactcc aacgctttat atgatttatc agttgcaaga aaagattagg
     4561 aacttcaaga aagaacccta ctttgaagga aaagcccaag taaccgctca aaatggcaca
     4621 tttgatgcca agcttgatcc caatgaaacc caagtgacgc aagaagcatt tgaagcg
//
LOCUS       DAZKCN010000141         4455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000141 DAZKCN010000000
VERSION     DAZKCN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.146"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /gene="bacA"
                     /locus_tag="ACG8K3_002541"
     CDS             <1..411
                     /gene="bacA"
                     /locus_tag="ACG8K3_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFP9146351.1"
                     /translation="ITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYNHNHRGFT
                     CYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFV
                     RAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            1701..2624
                     /locus_tag="ACG8K3_002542"
     CDS             1701..2624
                     /locus_tag="ACG8K3_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9146352.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2640..3179
                     /locus_tag="ACG8K3_002543"
     CDS             2640..3179
                     /locus_tag="ACG8K3_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP9146353.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3864..4037
                     /locus_tag="ACG8K3_002544"
     CDS             3864..4037
                     /locus_tag="ACG8K3_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146354.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
BASE COUNT         1661 a          673 c          758 g         1363 t
ORIGIN      
        1 attacttgtc tatttttgtt cggtatgatt gttggagcaa gtactccgtt aaatctgttt
       61 ccttttagtg ctagaggtat aaaagtggaa gctgcaagtt cacgatataa tcataatcat
      121 cgtggattta cttgctataa aatgaactat tatgttacta ataaaatgtg tagagatttg
      181 aaaaaaaatt ataataagct aaagactcct gctcaaattg catcttttat tcctattggt
      241 ggagttggaa catgggttat tacaaatgct tttagtagtg ctgttgatgc aatgaatgtc
      301 tttgtaagag ctgcaaatca aggaaaaggt gtacaattaa catataatgc tcactttagc
      361 aatacaacat catatcaata caatgattac gctcgttatg taataaaata atcagaaagt
      421 gaaactatct catgaataaa aagattttag ctgtaattat ttcaataata gttttaatca
      481 ctatgttctt catatttagg ctaattttca atataagctt acaaaaaagt attctttatc
      541 taattcctat agcatttatc ttagcaattt ttagaagtat ttacggaaat aaaaactcta
      601 aatagctaaa aataaatcat actaaatttt acaaaaagtg gctagcattt tctagccact
      661 ttttgtattg tggagcgtgg gttttgaggg ggattcaagg gggagcttgc gcccctttga
      721 caagcggtta tttgcaccaa atacggaggt atttggctag caaatataga gcttgccaaa
      781 cccttttagt gagttttgta gaaagtacct ttaaaggtac tttcacatta cgaacgatac
      841 gtccgtagga cgtactgaaa gggtgggact tatgagcaaa ttaatgcaaa atttggctag
      901 caaaaaagct aaaaaatata tgctaataat cgagcgaaat aaacactttt tcaagtgagc
      961 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgccgact
     1021 tttttaaaag tctaggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
     1081 acgtttgaaa cgcctaagta tataaatata taagaatagg tttaagagtg cttaaatcga
     1141 attttagagg gcatttagac aattttaggg ttgttagggc ttgccctgac cgcctgtaag
     1201 gcgttttaag aacaagataa gggatttata ctaggactgt cttaaaatgt cttaaacgaa
     1261 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
     1321 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
     1381 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
     1441 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
     1501 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     1561 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     1621 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     1681 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     1741 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     1801 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     1861 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     1921 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     1981 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2041 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2101 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2161 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2221 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2281 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2341 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     2401 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     2461 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     2521 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     2581 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     2641 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     2701 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     2761 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     2821 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     2881 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     2941 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3001 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3061 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3121 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3181 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3241 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3301 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3361 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     3421 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     3481 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     3541 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     3601 ttgaccattt tctctcaacg tggtaaaaat cccacttata agagaaaatg gtcaagaacc
     3661 taaacggtcg gcgaagaaaa cataccaagc accacaagca aaaaaagtta gagtaacttc
     3721 caaaaaagaa aaatttttta gaacaacttt tgaaaattta attttgattt atatccgttc
     3781 aatttaaaca tttgataaac tgaaggctag tatttaaaat gtgtatacaa gtggtagcgt
     3841 taaaaaaagt ggaggtataa cctatgttaa aaaaaactgt gttggtactg ggttctattt
     3901 ctctaggaat attaggtttt agccccatgc tattatccgt aattggaaaa aacaattcca
     3961 acggagaact taaattaaat tcaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     4021 aagagacgga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     4081 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     4141 atgaaaaata acaccccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     4201 gcaactgcag aaaatcaagc cttaaaaaga gcttttgact tagaccaatc gaaaatcaca
     4261 aagtgagccc ctgtatccct tggtagagag gctacttggc atagtgtaca ttatgtctac
     4321 taacctttaa aataacacgt tttataaaat aaaccctctt gacaatattt aattttctga
     4381 ttattctaaa tatatacaga aagcgctttc ctaggatttt atatctaagg aggaagtaac
     4441 aatgaaaaat aaaat
//
LOCUS       DAZKCN010000142         4243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000142 DAZKCN010000000
VERSION     DAZKCN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.147"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..734
                     /locus_tag="ACG8K3_002545"
     CDS             201..734
                     /locus_tag="ACG8K3_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146355.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(860..1537)
                     /gene="nth"
                     /locus_tag="ACG8K3_002546"
     CDS             complement(860..1537)
                     /gene="nth"
                     /locus_tag="ACG8K3_002546"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9146356.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1544..2242)
                     /locus_tag="ACG8K3_002547"
     CDS             complement(1544..2242)
                     /locus_tag="ACG8K3_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP9146357.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2510..3694
                     /locus_tag="ACG8K3_002548"
     CDS             2510..3694
                     /locus_tag="ACG8K3_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP9146358.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3700..4143)
                     /locus_tag="ACG8K3_002549"
     CDS             complement(3700..4143)
                     /locus_tag="ACG8K3_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9146359.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1135 a          817 c          672 g         1619 t
ORIGIN      
        1 ttttttataa gactatccag catatacatg tgtgatctaa catttagctt aaaaattaga
       61 aaatccattt ttcttttatc attacatata ctttatccat ttacttgttt ataattaaat
      121 cggctaaata cacactagtc ttttatttag tgccacttct taataatccc attgttatta
      181 tcaaataaaa aatacaatat atggttttga ataatttttt gaaagaaggc ttcattttga
      241 gttatctgat agtatgctta attatcataa tttgtctgca ccatcttttt ttatcgaaaa
      301 caatacctcg ttattccaag aataaaaaag ctgaaaaatt ttgtttatta ttaaataaat
      361 ttactttaat tgctcctatc cttgcattta ttattttttc tgtactttta agcactactc
      421 ttaaaggaaa atttatggaa agaagctctc atgcaatgat tctcactttt ttatggttgt
      481 tgttcaccag aatctatatt tttctgatga gcctgaaacc gcccaaatcc attagtttat
      541 gtttagttat aaatggtatt tttttattat cacttatcat ttttattaca cctttagata
      601 gatacgtcac ttatttatat aatcctttgg aatactggac ttattttata ggtatattgg
      661 aaggcattat tttctacatc gggtattttc ccaataaaaa taacaacttt tatttttatc
      721 gcaataaatt gtaataagtt accatcctag ctactgagta tgtacattta ctttttcatg
      781 caaaaaagat aaacgaaaca gcttttctcc ataataacaa gataattcat acatttattt
      841 gcaattaaag aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt tcttttagac
      901 gcatccgatt tttcccatct tgacatattg atagcaaagg acatacctcg cacttgggat
      961 ttcttgccgt gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata
     1021 attcttgtgg tactttccgc atcagtgttt cttctacttc catgaccgta gcatctagtt
     1081 tgcagatcct caggcgtttt gagacacgtt ccacgtgggt atctactgcg attgctggta
     1141 tcccgaatgc atcccccaat acgacatttg ctgtctttct gcctacacca ggaagagaca
     1201 tcaattcttc tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac
     1261 acgctttgat gttttttgct ttatttcgat acaaaccaat cgtttttatt ttaagaatga
     1321 tttcatcgat tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg
     1381 gcgttgcttt attcactgat acatctgtcg cctgggcact taagatcact gcaatcaata
     1441 actcaaatgg attattgtgc ttgagttctc catgtgcttc gggaaacatt ccatacatcg
     1501 tttctagagc ttccattgtt ttttgtttgc ttaacatatt ccgctactcc tccaaccaat
     1561 ttttcagtga gactttcggc aattcttctt gttgagctga ctgttctact tctctttgaa
     1621 gaagctgctg ttttcttcgt ttttggtctt cttccacttg ttgttttgta cgtagatttt
     1681 ttctttccca cgatagcaaa atgcggtcaa cgtaattaaa actataagcc tgatttaata
     1741 cggcttcccg cagtgctagc ttcaaaattt ccggctgata atggtcttct tctagccatt
     1801 gtccgatccg ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt
     1861 gatacactga ctggatttct ttttcttgtg ttttttcttc gtattttttt tcttgtgttt
     1921 ttaaatactc acctaataga tcatagatgg ggtaaagatt atatcgatct gcttttttac
     1981 cgttgacaat cgtcgtttct atcttgataa atccatttgt tacgagacga tccagtattt
     2041 gaaaaatctg atcttgtttg attcccatat ccatagcaat catctgcaaa tcagggaaag
     2101 tatctccttc aagctgtgcc atatgcaact gtagaacaaa taaaaattct tcagattgta
     2161 gaccgatcct atgataattt ttaagtaaaa gattagatat gactgtttgt ccagcgctta
     2221 agtaattttc taaattcaac atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa
     2281 acatgggaag caagaacagg tttcttttct atttacttca ctgatcggtc agatttacaa
     2341 tgtcgtctac ttgatttttg tttgttcatg tttttgatgc gtatcttcac ttttcggaat
     2401 aaataaatta agtagatttc gctgttcctt agaatgttga caaggaaaca aaattactat
     2461 acactatttt tgtttccaag ttaacattat tgcataaagg agtttttaca tgtacagttt
     2521 cacaaataaa cattctaaag tttttggata cgtctctgtc ttattcactg gattaggatt
     2581 tactatcgtc agtcctgtac ttccgttttt aacgctgcct tattctcact ccatacatca
     2641 gcaagctttc tatatcactt tgctcatgtc tgtttatgct ctagctgcct tcttatctgc
     2701 ccccattctc ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta ttatcagttt
     2761 attaggatca agtatcggat atttgctttt tggtttagga aattcgatat ggatgttgtt
     2821 ccttggtcgt atcattgaag gattgacggc aggagaaatc tccaccttat atgcttactt
     2881 tgccgacatc acggaaccta atgaaagaac caaagttttt ggatggatgg gtgctttagt
     2941 cggtatagga acaacgctgg gtcctattat agggggtcta ttagctgagt tggggaattc
     3001 tgttcctata tttattggag cactctttac tttcttaaat gccgtttatg gctacacatt
     3061 tatgctagaa agccttccaa taaaaaaacg ttcagtagat ttaagtttct cccatgtaag
     3121 accttttcac caattacaac aattattcaa gatttcatcc gttattcctt tattgacagc
     3181 aggttttgct gtttggttgg cagctggctc tcttcaatct atcttttctc aattttcgat
     3241 tgacgccttt cagtggaagg ctgggttggt tggtttaagc ttctctttga ttggaatctt
     3301 ggatactgtc tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt cagaacagca
     3361 gatcacaaga atagggatgt tcagtgaaat cctcgcctat ctattcatca cattatctgg
     3421 aattcttcta ctgccgattt tgtttttatt tgggattatc tgttatggtt tcggtgattc
     3481 tatttttact cctgtattca atggccaact atccaattct gtctcagata accagcaagg
     3541 tcttgtaatg ggcggtaccc aaagtataca atctctatcg cgcgtcatcg gcccgctaat
     3601 cgcaggacag ctttatgctg cagcaccatt tcttccgacg gtattcggat ttttgcttat
     3661 gatttttgcc tattatcact acaataaaaa atagtagctt catttgctac ttcgtataaa
     3721 ttctctgtat cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg acatattttc
     3781 aaatacagta gcatctcgct gtacccattt ttgatggagt tgctcgtgtc tattccttat
     3841 tttttgtccg attggcgtca attgaaaata gatttctttt ttattctctt cgctttgata
     3901 agtgtttatg atccctctag ctatcagttt ttttgttagc ttactgatgg ctcctctagt
     3961 catgtaacta gcattcgcta attttgtcac gttaggatca gggattttcc cgatatgttc
     4021 aatcacttct agttcattta gagtaaatcc tgcaaattct ttttccattt ctggtttatt
     4081 acgatgaata atctcattag ctaaatccag atattcttct attagcagtt gtcttttatc
     4141 catttctaca ccttcttttt ctttcattat attgtttttt tgtttcctat tcaacaaaga
     4201 taaaaatatt ttgtaggaaa acccaatctg aaaccaagaa aaa
//
LOCUS       DAZKCN010000143         4128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000143 DAZKCN010000000
VERSION     DAZKCN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.148"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1352
                     /locus_tag="ACG8K3_002550"
     CDS             51..1352
                     /locus_tag="ACG8K3_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9146360.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1379..1639
                     /locus_tag="ACG8K3_002551"
     CDS             1379..1639
                     /locus_tag="ACG8K3_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9146361.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2638..3873)
                     /locus_tag="ACG8K3_002552"
     CDS             complement(2638..3873)
                     /locus_tag="ACG8K3_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9146362.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1182 a          667 c          863 g         1416 t
ORIGIN      
        1 cgttgtcatg caatgaatgg ttcatatttt attgaaaagg agtggtttta gtggaaaagc
       61 tgatgaaatg ggtcgaagag aaacttgtcc caccaatggc aaaaattggt acacagcgtc
      121 atttattagc aattcgtaat ggggttgttt caacattgtc attgattttg attgggacat
      181 tttttatggt ctttatcaat ttaccgtttc ctggatggaa tgaatttatt gccccttatt
      241 cggcaacgat tgttttaccc tttaggatga cgatgggatt gatggctatt tatgcaactt
      301 ttgttatggg ttcggatcta gctaaatcat atggattaga tagtgtgaca ggtggtatat
      361 tatctttagg aacgttcttt atgttacaag ttcctgtaaa cgttttaact cctgaagagg
      421 cacctcttgg ctgggtctta cctatgagtt ctttgggagc atctgggatg tttgcgggga
      481 tcttatctat gatttttgca gtggaagtat atcgtttctt taaaaatcga aatattacta
      541 tcaaaatgcc agaacaagtg ccacctgctg ttgcccgtag ttttgaggca ttgattccag
      601 gtgcagtcat tttaacaatt acttggctga ttcgaagcgt cgcgggcttt gatgtaaacg
      661 ctgccttatt atcgttattc gagcctttaa ccaatatttt agggaataat ttgcttggtg
      721 ttttactacc aatgtttttg atccatttgt tgtggtcatt tggtatccat ggcatgagta
      781 ttgtaggggc tgttgttcgt ccaatgtggc tgatcatgtt ggatgagaat gcaaaggcat
      841 tggcagatgg tacagctgcc acccaattac cctatatcgc tcctgaacag ttttatcaat
      901 ggacggtaac aattggtggt gcaggagcaa cgatcgtggt atctgttttg tttatattct
      961 tctgtaaatc aaaattcttg aaagaggttg gtcgtttttc tattattcct ggtatcttca
     1021 atatcaatga gcccataatt tttggagctc ctatgatgtt gaatccttac atgttcatcc
     1081 cgttcaactt agttccttta gttttgacta ttatcagtta ttttgcagtc aaattagaaa
     1141 tggtcaatag atttactgtt ttgccaacat ggacattacc agctccaatc ggcggttatc
     1201 tttcagcagg aaatgactgg cgtgttgtta tcttgatcat gatcaacaca ttgatagcat
     1261 ttattatcta ttatccattt gtaaaagctt atgataaaaa gatgctgtca gatgaacaaa
     1321 gtcgtggaga aaacgaagtc cagactgtct aagtttggtt tttagaaggt gggaaacaat
     1381 gtattggctg attttatttt ttgtttttat ctttttatta acggctagtc accttatatt
     1441 gaatatgcta gctacatatc atattcaaat caatcgatgg atttgggcac tggcatcatt
     1501 tttgatcgtg attcttccaa agatcattgt accgcatatg aatgttttgt tttcatgggg
     1561 gacatatgta ctttgtggaa tttttgctat caattttatg atcgagcagc atagatggtt
     1621 tgtcacatcg aagctttaaa tgttctaaaa gtggttgtaa cagaagtggt tagcttcaag
     1681 aaataagaag aaatttacga aaattgcttc ttaaattttt gtgaaatgtt ggcttatttt
     1741 tcgtttggtt gaacttatta ccgcagatgc aagcttttga ccaccgttta ttcggaatat
     1801 gggactgtga caaggctttt gtcacagccc ttttttacat aaagattatt attgttttaa
     1861 ctgttttggc agaaagaagg aggaaaaaat ggaaatttta tcagcaatgg atttagaatt
     1921 agtagatcta ctaccaaagt gatcaagtca aatatagatt tgggagctta caaccatata
     1981 gtaggggctg cagtatggtg tatgaatggt aatatgtatg atgtgagtgt ttattcaaat
     2041 catgaaacgt gtatagaaat aattgtgctg ggaatagcaa tcagtcatgg tgaacgtgac
     2101 ttttagtgta tcgttgctga cgacatagaa cattctgaca tgatctactc aatatgtggg
     2161 gattgctggc agtttatata ggagtatgca tcggattgcg agatcattgt ctcaacagaa
     2221 gagggaggtt gattatcaaa gataaagtgg taaatcctac atttgattta gtaattgttg
     2281 aggggactag cggcacaaat ctctacaaag cgatgcttct agatctatat gacacatttt
     2341 aagaaaatgt atttgcctaa ttattatggt ctttcttttg aagaaacgtt gagatgatcc
     2401 aaccagagaa aggtgtcaaa gaatttgact aagaataaat ggcagacgct acctatgctc
     2461 atttggatag cgtcagatct tttattttaa ggatgatttt gtgtttttta gacaccgtaa
     2521 tttaagtaac tctaactttt caatccacta tttacttttt ttgtattttc actgtctggg
     2581 tctcgaatag cgaaattact gatgagtctc tattaaaaaa gctttttcta cttgaaacta
     2641 tattgggtgg ttgctcagga aggtttcgat ttcttgaatt gcttgttgtg gaccatttat
     2701 tggaaaaacg gcttgcagcg tgcgtgcctg ctgacgaatc ttgggtgaag aaacttcgcg
     2761 cagagcaatc tgcagttttc tttcagtcac tggttttgtg agggcgattg cgtttccgta
     2821 ttttacacaa tagtcaatat tacagctttg ctcaaattgc atccccacac cgataaacgg
     2881 tttgccggat tcacaggcag tttggacggt cccttccccg ccgtggataa aagaaaaatc
     2941 aatgaattca tgcaaaagat gactagacag atattctgtt attaaaagat cttctgagtc
     3001 agattgaatg tcatcaagca gatagtcaat aggtgcaata accgctagat ccggttgttt
     3061 ttgtaagaag aacaaaattt tgcgtaacat acgcggattg cctgaactac ccattgcgaa
     3121 atagataata cgttttcttt cttgttgctt tcgctgtata aaagttagca cttcttcagg
     3181 caaggaagta ctgagcttag caaaaattgg accaacgatt cgataatttt ccggcagctg
     3241 ttcttcttcc aaaaatagtt taggcgttgt gatgagattt agatcagcat ccatcatttg
     3301 taaacttgtt tttggtaaag gaagcatttc tttttgtgca gcgtgtttga aggcattggg
     3361 gatccatcgg gtatttacta tacgcttttt tgtccagtgc caaaggggat tgcttaaagg
     3421 gccgaagaaa cgtaactcgt ggggcatcag cgcatgctct gattgaaaaa aaggttcact
     3481 caaagcatag ggtttaacat atactaaagg aatcttttta ttgcgggccg ataaaaaaat
     3541 tgtcacattc gaacctgtca caatcagtcg aggctcaaac ttagcaatca gactctgttc
     3601 actagtgata cgagaggcaa ccatttcttt ggtaaaaggg tttttaagtg tacgcaactg
     3661 gtcaaatttt atgatttgtt cttcttgttt tttcgtaagg atcggcgcta gtaaatgata
     3721 ctcaaagcca ctttctgtga tcaaaggagc aaaagtttta gaaaagccaa agaatacaca
     3781 gtaataacgg tctttcatag ctttggcaac ctcaatcatt cgtgttgtct cagctaaatt
     3841 gaatactgca ggagcaaaaa gaattgtctt catgaaatcc tccttattgg aatgagtcta
     3901 tcccttcgtt tcttactagt atactaaaaa acacagcgtt cgagtagcct atgtatattt
     3961 tgaatattaa actgtctctc gctgttttca caacttctaa atttaaattt tgtaatcaag
     4021 ttgaaactcc aattttcaaa aaaacacgca acaatttttt gatcgattta tatgttgtgc
     4081 cagataaata cgagaagttg tgtcattaaa aataaacatg agaacata
//
LOCUS       DAZKCN010000144         4053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000144 DAZKCN010000000
VERSION     DAZKCN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.149"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..569)
                     /locus_tag="ACG8K3_002553"
     CDS             complement(111..569)
                     /locus_tag="ACG8K3_002553"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9146363.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(740..1885)
                     /locus_tag="ACG8K3_002554"
     CDS             complement(740..1885)
                     /locus_tag="ACG8K3_002554"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9146364.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1898..2815)
                     /locus_tag="ACG8K3_002555"
     CDS             complement(1898..2815)
                     /locus_tag="ACG8K3_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP9146365.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3243..3806
                     /gene="efp"
                     /locus_tag="ACG8K3_002556"
     CDS             3243..3806
                     /gene="efp"
                     /locus_tag="ACG8K3_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9146366.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1269 a          760 c          771 g         1253 t
ORIGIN      
        1 aaactagcta tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat
       61 acgaagataa gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct
      121 acttcagacc attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct
      181 agtgtatggc tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct
      241 tcaagaacat ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga
      301 ttcaatacag tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa
      361 tctgcgtgat tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg
      421 tccatgtgtt ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca
      481 tgcgcacccg tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca
      541 agattaaaac tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt
      601 tttgatgctt ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga
      661 ttctaacact tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct
      721 aagtcgttta ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta
      781 tgagatcctg accatactca attcctacag aaagacggat caagccgtcc tttatgccag
      841 cctcttctcg tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta
      901 aactttctac gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg
      961 gaatagcaga ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct
     1021 gctgcttagc aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta
     1081 ctgctggatg agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca
     1141 tacgaacact tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta
     1201 cggctccgat agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag
     1261 tcactaaacc agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa
     1321 cgatatcaga accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag
     1381 caataactat caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct
     1441 ttagaagcgg attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt
     1501 gttctatttt atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa
     1561 gaactttgtc aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat
     1621 cgccagcttg aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga
     1681 atcctcgaac accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat
     1741 taccagatct tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg
     1801 atgtttgata aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt
     1861 ggatcaattt tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata
     1921 aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac
     1981 gaatttctct taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt
     2041 gttctcgtgc taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct
     2101 gattgattga aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt
     2161 cgtgaggggc tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc
     2221 gaatgtcggg atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc
     2281 caattcctgc tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta
     2341 acgtatgata gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt
     2401 ttgatatctc ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac
     2461 taggagaatg gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa
     2521 acttctctgg aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc
     2581 caattcctgt atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt
     2641 gggcaaatcc ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat
     2701 ttaaatattc tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt
     2761 caaagcgata aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg
     2821 cctcctaaaa aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca
     2881 tagagaaaaa ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa
     2941 ttaatagaca tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat
     3001 tgtttttggt tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc
     3061 atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac
     3121 ccgtgtttat ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt
     3181 ttctggccgg ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa
     3241 taatgatctc agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga
     3301 ttaaagttct ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga
     3361 aactgaaaga tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat
     3421 ttgaaaaagc acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag
     3481 ctttcttcat ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag
     3541 aagaaatgaa atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag
     3601 tcatcggcgt tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca
     3661 tcaaaggtgc aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag
     3721 tcaatgttcc tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt
     3781 cttatgtaaa acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga
     3841 gcttgacctc ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc
     3901 agcaaattga aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa
     3961 aagctagaga aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt
     4021 gaaaatatcc gaactattcc gaactatatt gaa
//
LOCUS       DAZKCN010000145         4032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000145 DAZKCN010000000
VERSION     DAZKCN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.150"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1264)
                     /locus_tag="ACG8K3_002557"
     CDS             complement(62..1264)
                     /locus_tag="ACG8K3_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146367.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(1251..3038)
                     /locus_tag="ACG8K3_002558"
     CDS             complement(1251..3038)
                     /locus_tag="ACG8K3_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFP9146368.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(3051..4004)
                     /locus_tag="ACG8K3_002559"
     CDS             complement(3051..4004)
                     /locus_tag="ACG8K3_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9146369.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
BASE COUNT         1438 a          720 c          695 g         1179 t
ORIGIN      
        1 tccggctatc tctgacagct ggagttttta tgtttaaaaa tgaattagta catcggtatt
       61 cttaaaagta atttagttcg tcttttgatt tctcaggagg gatcaaaaag gcgaaagcac
      121 taagtatagc aaaacttgtg atgatttgcg tacgatacgt aaaccaataa tgaatctgtg
      181 agtgattagc taaaatattg taccaaatat acggcgttac agcagctaaa acaaacaaaa
      241 ctagaagttg gaacggacgc cagctatttc gtttacgata agcaaaatag agaaaaacaa
      301 gacaagtaat caatagcaat aatattgcta cttttggata catcaatcta atattgcttt
      361 tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc ttccgttcga aagaggattt
      421 ggttgattgc atcttttaca atgtttttgt tcaatatgat cgatgatagg atccatttag
      481 ccacccatgt caaaccatag cctaatcccc aaaagatacc ggtaaaaaca aaactgccta
      541 cttgtttgct caatgaactg cgtttatcca ttaaatactt atttgttaca taataaataa
      601 tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa caagtcaaaa aagttagcta
      661 aagaaccaat gacaaagaaa tacaagaata attgttggtt ggtgttggag agttgtggtt
      721 ttgttaaaaa tagtaataca gacaataaag tcaaaatcca aatatttgaa aattgcatac
      781 tgaagaaaaa ggttgcagca tttcctatag caagcgaaat caacaaggct gtcgttaaat
      841 agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc aatcagcaga aaaagaatgg
      901 ctgcgtaaat cattcgaata ctccccaaac tcatccctag cagtaaaggt cgtaaaaaaa
      961 gctgataacc atgccaataa cggggataat ccgccataga catcgcgttc tccaaggagt
     1021 tcaattcagg atctgctttt gtttttcgta tcatcaaatg atcagtaaaa ttatctaacc
     1081 gagtgcctag catatttcct tgattgatag aaggatacat tccttctttt tcaaccatct
     1141 gtagagattt ttctgcattc gattcaatag agtgctgcgg aatgaaaaaa gtaattgtca
     1201 gtagtacgca atagctagcg atcaagagaa caaaagcaaa aaatatcttt ttaaatagtt
     1261 tcatgagtat ctcctatatc tgttttcatt ttttctcttt tatctagtaa aaaaggagag
     1321 aggatataaa caggtagacc taatgctaaa acaaacacat accgtaaact aaaagccaca
     1381 ggagtagcaa tcataatagt agcaaaaaca agcataccgg gaagaaaaag gagtaaatac
     1441 cgatattttt tcttaaagat tgctaaaagt gcactgccga tagctaacca cagtaatgtg
     1501 ccggaaccaa agaaatcacg agcatcgatg atttttttca ttcctgacaa tccagtaaga
     1561 tgttcaatca aatctacttg cttcatacca taattattcg tgtttacacg agtatctaaa
     1621 aagccgtaac tattttttgt accaggtacc cagaacccga acgtttctaa aatataagca
     1681 tctgtataga tttttatatt acttggtaaa tttcttgccc atagtgatag aaattctttt
     1741 ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc acttgattga gtctgcaaga
     1801 ctaggagaat acgcttcata atcttttatc gggagtagct gattcatgaa tgctttatct
     1861 ttcttagaca agtcaccatc cataacaata gttctagaaa tttgctgcaa aggaacacct
     1921 aattgttcag cagtttctgt aggaatagca taacgagcca tcaacgggtg gatgagtagt
     1981 ccagcaatca ctgtataacc cagcaccact tgaaagaatg cttgatacct tttttgtttc
     2041 aaaataatca agccgcaaat caaacctgtg ccggttacta cataaatccc attatttctt
     2101 agataggtgg tcagtaaagc aaggatgatt acgtaaatct gattgagaat cgttggcttt
     2161 tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata aaaagcaagc gatagagaac
     2221 aaagtatctt tttgcatgtt caaggcgtag attggaaaaa ccggtgcaaa tgcaaaatag
     2281 cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt ttaacagcca gcaaagagaa
     2341 taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg aaaataaaaa tacaccgtga
     2401 ttgatcgtat cagttggtaa atcatgaaca aatagcttga ccagtaaggt gaataagact
     2461 ggatgatgat tgcttatagg aacatcaccc atagattggg cgaccgaact taacgaatca
     2521 ggcaaaacca ctccaggata caatgccaac agataaggaa gccaacacag aactaaaaag
     2581 acagtaaaga ataaaaaata ttttcttgaa gagaaagcct gtgtgttttg agcagtaaaa
     2641 aatgtttcca gttttggcat tgataaataa atcgattgta aaaataaaaa agaatacaca
     2701 aaaatggaga aataaacaag aaagtaaaaa atacttccat cagcaatcgt tgtctttgta
     2761 tagtttgctg agacatactg aaattgtatc tgtctgccaa tagaaagtgt aagagcaaaa
     2821 gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt tttttattga aaacaactct
     2881 attccatatt gcttaaaagc aagtagcaaa accatggcta aaccaatgat tactgttgga
     2941 ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac ctgacgcaaa taaagttgca
     3001 aaaaaagtag gaaatagatt ctcctttttc ttatgcataa aaaaggcctc ctacttcatt
     3061 tctttatttt gatattcgtg gtagacatgg tataaattca attcatattc tttcttggca
     3121 tttgtgacga ccgtatccaa aatcaaccct gttactaatg aaagaagggc taaagtcatc
     3181 aatccagtac ttaaaatcgc agaagggatg cgtgtaataa agcttgtcag catatattca
     3241 cgaatcacag gaatccccat gaccaatcca aataaaaaga agaagatggt ccagatccca
     3301 aagaaaagca aaggtttata atctttgcac attttgatga tcatcatgat cactttgaat
     3361 ccgtcagaga atgtattcaa ttttgattcg ctaccttcag gacgatcgcg ataatcgatt
     3421 ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa tcgtcaattc tgtttcgatt
     3481 tgaaaaccag aactcataat agggaagctt ttgacaaaaa ttcgattgaa tcctcgatag
     3541 ccggtcatga catcgcgaat ttttgtttta tataagtaag taattgtatt tttaaccaga
     3601 ttattcccaa aatcatggaa aggacgctta ttttcatcaa aataggttcc atttgataga
     3661 cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt tctcaagcaa accatgtgca
     3721 gcttctgcag gataagtatc atcaccatct accatcaaat aataatcggc atcaatatca
     3781 aaaaacattt gacggattac attccctttt ccttgacgtg gttctttctt tacgattgct
     3841 ccgccttgaa cggccaattc gtatgttcga tcggtcgaat tattatcata gacataaata
     3901 tctgcttctg gtaattccct tttaaaatct tcaataacag ttgaaattgt cgcctcttcg
     3961 ttataacaag gaatgagcac ggcaattttt tcacgtgatt ccatttgtca ctctccttat
     4021 ataataaaac ta
//
LOCUS       DAZKCN010000146         4022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000146 DAZKCN010000000
VERSION     DAZKCN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.151"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..204)
                     /locus_tag="ACG8K3_002560"
                     /pseudo
     CDS             complement(1..204)
                     /locus_tag="ACG8K3_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            353..2320
                     /locus_tag="ACG8K3_002561"
     CDS             353..2320
                     /locus_tag="ACG8K3_002561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146370.1"
                     /translation="MKRFLKIFIFLEIILFVYIFNTSIYNIYEKNNIATDNLKGYVLE
                     ETSPEILDKFYTIFTEEYSQNKLELINNKLTSTDKSVYDLYCYPLNEFVQKQPISNSI
                     LLQYHKLQKEDFLDSVGVFYTDLQANEIREIESQLAVTISVFENEAIPYSMILELYLL
                     NFVILFVVLQIIYCIYTSDSLKKKGIKKSMGFSAMHILKEQINNIVKYFIVICSVLFI
                     LLNLYYALTNRFNISYLTISIFFFAIILVINILCVLITSFLIKFVSLEAMIKNKTLNS
                     GTNLIVQTIKIVFSVIITITTISLLNQGNEFKQSQQDILDYKYLDGYFTANGFNSLEY
                     EYALENPEQFEEYSNSMLELYNNHHALLCDYAGSSEAQGEFSKSRPYYIQQTIIANRN
                     YISEFSNIQVNGKSLNEDIYDTPTILVSRKYKSDETFIKEHITQEYFRLMNYNQFYGI
                     PEKERHINEFNIVYVDDNSTIKVNTENGFSDMSEPIIIIDTGNFAGLYYLDSLNTRNL
                     FFQMDSREEFSSLLAEYGLKQLVTAGTLLTPYLMQLENVTFVLKTLTMFAIVFIISLL
                     FILYISNYVDIFVNRKKYATKEIMGFSHFKTLKNRYIFWGIELIASCILTAINYNFAC
                     LFAIILIDYIFCELLYRIYILNALYEIEKGA"
     gene            2323..2973
                     /locus_tag="ACG8K3_002562"
     CDS             2323..2973
                     /locus_tag="ACG8K3_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9146371.1"
                     /translation="MSLITLKNIKKVYGKNQILFENLSLEIEKGEFVGLVGKSGAGKT
                     TLLNIIGLITDISEGIITIDGQQKLSVNSRNAMLLRRYTIGYLFQNYGLIEDESVLWN
                     LKLALEYKKMKKEDKLKVINEYLKQFNLSDMLNKKIYQLSGGEQQRIAIIKLILQGSQ
                     IILADEPTSGLDKENEQVVMKLLKELNEKGITIIMVTHNMDLRNYFSRVINVETFR"
     gene            complement(3433..4022)
                     /locus_tag="ACG8K3_002563"
                     /pseudo
     CDS             complement(3433..4022)
                     /locus_tag="ACG8K3_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1432 a          546 c          664 g         1380 t
ORIGIN      
        1 ttgaatggcc ctttttacaa gctgcgcatc ttttttattt ccgcaagaat ggccaataat
       61 ttcacgattg aataaatcaa tcatcaaaca aatataagcc caatttgtcc cgacccgaac
      121 ataagttaaa tccgtgacaa ccgctgacaa tgcttttgtt tgagtaaact ttcgatctaa
      181 gtgattcgca attttctttt gattgctttg gcacgatcat aaagacggga gcagatattg
      241 tctattgata taatgatagt agacaaacag agttggctga ttagctaaag catatcgagt
      301 ataatttgaa tattagacgc atgatttatc agtgtaagaa agggtgagga ttatgaaaag
      361 gtttttgaaa atatttatct ttctcgaaat aatacttttt gtatatattt ttaatacttc
      421 tatttacaat atttatgaaa agaataatat tgcgactgat aatttaaaag gatatgtttt
      481 agaggaaaca tcgccggaaa ttcttgataa attttatacg atatttacag aagaatattc
      541 ccaaaataaa ttagagctta tcaataataa acttacttct acggataaat ctgtctatga
      601 tttatattgt tatccgctaa atgaatttgt gcaaaaacag cctatatcaa attctattct
      661 gttgcagtat cataaattgc aaaaggaaga ttttttggat agtgtcgggg tattctatac
      721 ggatttgcaa gccaatgaaa tacgtgaaat agaatctcaa ctggctgtaa caattagtgt
      781 ttttgaaaat gaagctatcc cgtatagtat gatattagag ttgtatcttc ttaattttgt
      841 aattttgttt gttgtacttc aaataatata ttgtatttat acatctgata gtttgaaaaa
      901 aaaaggtatt aaaaaaagta tgggcttttc tgctatgcat attttaaagg agcaaatcaa
      961 caatatagta aaatatttta ttgtgatatg ttcggtgtta tttatcctgc tgaatttgta
     1021 ttacgcatta acaaatcgtt ttaatatttc ttatttaaca ataagtatat ttttctttgc
     1081 gattattttg gttataaaca tactttgcgt tttaattacc agctttttaa ttaagtttgt
     1141 ttctttggag gcaatgatta aaaataagac tttaaatagc ggtacaaatc ttatagttca
     1201 gactataaaa atagtttttt cagtaataat cacaattacc actatttcat tattgaatca
     1261 aggaaatgaa tttaaacaaa gccaacagga tattttagat tataaatatc tggacggata
     1321 ttttacggct aatggtttta attctttaga atatgaatat gcattagaaa atccagaaca
     1381 atttgaagaa tattctaatt cgatgttaga actgtataat aatcatcatg ccttgttatg
     1441 tgattatgct ggtagcagtg aagcacaagg ggaatttagt aaatctcgtc catattatat
     1501 acagcaaaca ataattgcaa atagaaacta tattagtgag ttttcaaata tccaagttaa
     1561 tggaaaatca ttaaatgagg atatatatga tacgccaaca atattagttt cccgtaaata
     1621 taaaagtgat gaaactttta ttaaagagca tataactcaa gagtattttc gactgatgaa
     1681 ctacaatcaa ttttatggga tacctgagaa ggaaaggcat atcaacgaat ttaatattgt
     1741 gtatgtcgat gataattcta ctataaaagt taatacagaa aatggctttt cagatatgtc
     1801 agaacctatt atcattattg atacaggcaa ttttgcaggt ctttactatt tggattcttt
     1861 aaatacaaga aatttatttt tccaaatgga ttcaagagaa gaattttctt ctttattagc
     1921 agagtatggt ttaaaacaat tagttacggc aggaacatta cttacaccat acttaatgca
     1981 actggaaaat gtgacctttg tccttaaaac actcacaatg tttgcgattg tctttataat
     2041 atcattactg tttatcttgt atatttcaaa ttatgtagat atttttgtga atcgaaaaaa
     2101 atatgcaact aaagagatta tgggattctc tcattttaaa acattaaaaa atcgttatat
     2161 attttgggga atagagttga ttgcatcatg tatattgact gctataaatt acaattttgc
     2221 ttgtcttttt gcaattatac ttattgacta tatattctgt gaattacttt atagaattta
     2281 tatcttaaac gctttgtatg aaatagaaaa gggggcataa gtatgtcatt gattacatta
     2341 aagaacataa aaaaagtata tggtaaaaat caaattttat tcgagaattt gtcgttagaa
     2401 atagaaaagg gtgaatttgt tggtcttgta ggtaaaagtg gtgcaggtaa gacaactctt
     2461 ttaaatataa ttggattgat tacagacata tcagagggaa taattactat tgacggtcaa
     2521 caaaaattat cagtaaacag tagaaatgca atgcttttaa gacgatatac gataggctat
     2581 ctatttcaaa actatggact tatagaagat gaaagcgtat tatggaattt aaaattagct
     2641 cttgaatata aaaaaatgaa aaaggaagat aaactgaaag taatcaacga atatttaaaa
     2701 caatttaatt tgagcgatat gctcaataaa aaaatatatc agttaagcgg tggagagcaa
     2761 cagcggattg caattatcaa attaatttta caaggaagtc aaattatact agccgatgaa
     2821 ccaacttctg gacttgataa agaaaatgaa caggtagtga tgaaacttct gaaagaattg
     2881 aatgaaaagg gcattactat aattatggta actcataaca tggatttgcg taactatttt
     2941 tccagagtta taaatgtaga aacttttaga tagtttatta aagggagata tacttttttg
     3001 tgcaatctcc cttgttcttt ttctgtcata tcaagactcg ttcaataatg gtaaattcgt
     3061 ggtaaaatag ctactttttt agttgatgaa tagcccttaa atactgtatt tatgcggata
     3121 atcggatttt ttataccaag tttggatata tcaacaaatc atttgataat tatgatcttg
     3181 gttttataat taatttaaaa aaaataaaaa ctatcgatga cttactcaca aataatttag
     3241 ttctcaaaaa agcgtactgt gatacattct aatgacgaga gcgtctttac cggaaaaatc
     3301 aatcttttag attcaaaaaa actagtactt tctataatag attacgaaag catcacaaag
     3361 acgaacaaaa aaaacaataa atatagatgc agactgtaga catcatccat aaaatggact
     3421 ttgtctacag tctgaaacga gatctttctt cttacgtgaa ggaagaatat ccagtaattt
     3481 cccagtatcg ccatccatac aagaaaaaga atagggaccg acttgatttt tcaatgaacg
     3541 aaattcatcc accaatgaac agcgaggcaa ccaattcctt ttcacgtttg tcatggggag
     3601 cagagaagcg agtgttcgcg aaacggttga ccaagaaacg ccgcaatgtt tagaaatcgt
     3661 cgtcattgct tgtttttcag tcaattcctc caatatttta taatgtactt gtttggcgat
     3721 aaaacagttt ttttcaacaa tgtaggtatt cgccgtaaaa taggtgtgac agccacgaca
     3781 gaggtaccgt tgctttgcca aagcaagata agtccgttga ttcgcacact tattgagaag
     3841 gaccagcgat tttttctttc cgctcttaac gatcagccca tgttgcttca cctgtttaca
     3901 ctgaggacag cgctgaatcg ttttaatgag cgtggcagaa acaacgatac tgttgattga
     3961 atgtattttt ttcttctcga catgagtgat ttgaatgttt ttatctttga ttcctagtaa
     4021 ta
//
LOCUS       DAZKCN010000147         3883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000147 DAZKCN010000000
VERSION     DAZKCN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.154"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1023..2036
                     /locus_tag="ACG8K3_002564"
     CDS             1023..2036
                     /locus_tag="ACG8K3_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146372.1"
                     /translation="MTKKNRVTIRDVAQAAGVSISTVSKALNSLDGVSPETRVEIQNI
                     ASRLNYVPNLMGKQLKSGKTKMIGVYTNRISGPYFNSLIDEIAFESKLHGYGLNVIVS
                     DERDVIMSSLLGNFVDGAIVVEDVLNQKDIETINSNGVATVFINRIVADKKVGSVAFD
                     SFEKGYLAGNYLLNLGHKKIGYVNGVDDIYDNDQRFQGFKAALSKVGLTYNEDFTIAG
                     NFVEEDAYANTLAFIDNQPAELPSAFLAANDMSAVGLIKALTEKNYSVPEDFSVVGFD
                     DVEVLKYFKPSLTTIHNSTKEQGKKAIEHLLEMMGGKSVGRVIEVKGTLMIRETAIEY
                     TGE"
     gene            2043..2393
                     /locus_tag="ACG8K3_002565"
     CDS             2043..2393
                     /locus_tag="ACG8K3_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9146373.1"
                     /translation="MYVGIDVGGTSIKYGLVDSKGNIVAKDTLVASFEKEELLQNLAD
                     IVEKYVSSSPETIEGVGISMPGIVQEDGFLLTAGAIRSCYGINLKEEMEKRTGILTKE
                     VIDSNGANLDWENK"
     gene            2547..3692
                     /locus_tag="ACG8K3_002566"
     CDS             2547..3692
                     /locus_tag="ACG8K3_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HFP9146374.1"
                     /translation="MATERSFATEENKTNHVSAETALVATSKTTVSKDNPTVFDFPFN
                     GEDNRMHRADGWSNGNMFNTTWRASQVNFEDGKMQLSINKDEENTKIPYISGEYRSNQ
                     NFHYGLYEVNMKPIKNDGVVSSFFTYTGPSENNPWDEIDIEFLGKDTTGVQFNYFTNG
                     LGNKEYYHNLGFDASEAFHTYAFEWLPDSITWFVDGEKIYTATENIPQTPGKIMMNVW
                     PGTGVDSWLNPFDGTVPLKAEYDWIKFTQYKIVEEDVTPKPEEPTTEPTTEPTTEPTT
                     EPTTEPTESSTTGSSTTESSTTNSSGNTTPPATTETTNTTSSSTSQSSKAAGKTTTPK
                     NNSTTSKKDLPATGENTHASYWTAFGGMLILAGLGGALFFKNKNRKA"
BASE COUNT         1332 a          623 c          814 g         1114 t
ORIGIN      
        1 acaaaaagaa agatcttttg cccattttat ttttataaaa tgggtaggtg acgttcgcgt
       61 aaagcaaatc gatacaatcc aatggttttg gcgaatcaga tttaccattc taggcttgtt
      121 taatacagac atcgattgtt attttgaaaa tttatatcct tattggcaat gaaatggata
      181 ttctccatat caatagaaat cttcaagata attttcggaa atatataaga gttaatcaat
      241 tatgatcata tagtaggtaa aaattaggac agccttcaat tatatgggaa accaatataa
      301 tcaagtaatt attcgggtaa cacaaatact agagtactca tttcttataa ctgttttaca
      361 cctaaaaatg agcttatcgg aaactttgca acagaaccag ttagctgtta catttaagat
      421 aatttcaaaa tcttgatcta ttaattagaa agtagggggt aaggtgggga aaaagaattt
      481 tgttttattt ttatttgaaa cgatggtcca gctttggtcg ttatttttat ttctgttaat
      541 tatttataaa tacttttttg gaactagttt ttcttttatt acgatcactc ggccagaatt
      601 ttatttaacg ggtttagtta gtcttgtttc tacgatcgtt ttatattttg tccgatcgcg
      661 aaagtaaaca aataaattct taaagccaac tccctttttg taaggagttg gcttcttact
      721 ttcctccgcc cacaagttca tttacctaac gtgtgaaaaa cattcccata atttctaatt
      781 aaataattta tttttattat aaaaacgttt tccttttctg gtaaaagagc aggcgttaga
      841 aatccccaag ctaaaaggtt tttccctgat tcaaacacga aaaacaaatg tatacgttaa
      901 caaaggcgtt aaattagctt ttatttttta taaaaaaagc ttgtatccaa aaaaatacag
      961 tgttatactt caatcgaagt aaagaaaaac gttttcttta aaaagtacat gaagtgaata
     1021 aaatgacaaa aaaaaatcgg gtaacgatta gggatgtggc acaagcagct ggcgtttcaa
     1081 tctcgacggt ttccaaagcg ttaaacagtt tggatggcgt ttcacctgaa acccgggtcg
     1141 aaattcaaaa tattgccagc cgtttaaact atgtgcccaa cttaatgggg aaacagttaa
     1201 agtctggtaa aacgaagatg atcggggttt atacgaatcg gatcagcggt ccttatttta
     1261 actcactaat tgatgagatt gcttttgagt ctaagttaca tggatatggt ttaaatgtaa
     1321 ttgtctctga tgagcgggat gtaattatga gtagtctgtt aggtaacttt gttgatgggg
     1381 cgattgtggt ggaggatgtc ttaaatcaga aagacattga aaccattaat tccaacggcg
     1441 tggcgacggt ttttattaac cgcatcgtag cggataaaaa agtcggcagt gtggcctttg
     1501 attcgtttga aaaaggttat ctggcgggga attatttatt aaacttaggt cacaaaaaaa
     1561 ttggttatgt caacggggtc gatgatattt acgataacga ccaacgtttt caaggattta
     1621 aagcagcctt atcaaaagtc ggtttgactt ataatgagga ctttacgatt gccggtaact
     1681 ttgtagaaga agatgcctat gccaatacgt tagcctttat tgacaatcaa ccagcagagt
     1741 taccgtcggc ctttttagcg gcgaatgata tgagtgcggt ggggttgatt aaagccttaa
     1801 ctgaaaaaaa ttattctgta ccggaagatt ttagtgttgt tggttttgat gatgtggaag
     1861 ttttgaaata tttcaaacca agcttaacaa cgattcataa ttcaacgaag gaacaaggta
     1921 aaaaagcgat tgaacattta ttagagatga tgggtgggaa atcagtcggt cgtgtgattg
     1981 aagttaaggg cacgctgatg attcgagaaa cagcaataga atacacagga gagtgattga
     2041 gtatgtacgt tgggattgac gttgggggca caagtattaa atacggttta gttgattcaa
     2101 aaggaaacat cgtggccaaa gatacgttag tcgcgagttt cgaaaaagag gagctcttac
     2161 aaaacttagc ggacatcgtg gagaaatatg taagcagttc gcctgaaaca atcgaaggtg
     2221 tgggaatcag catgccaggg attgtccaag aagatgggtt tttattaacc gctggggcga
     2281 ttcgttcttg ttacggtatc aatctaaaag aagagatgga aaaacgtacg gggattttaa
     2341 caaaagaagt gattgattca aacggggcta acttagactg ggaaaataaa taagtcacaa
     2401 agaaacgtca aacattaaca gaaaaataaa tgtttggcat ttcctttacg aaaaaatgaa
     2461 aacgattgct taaaaacaat aactggaggg aatttatgaa taaaaagagt gcaattttac
     2521 tattaagctg tggggtattt ttaagtttgg ctactgagcg gagcttcgca acggaggaga
     2581 acaaaacaaa tcatgtgagt gctgaaacgg cactagtcgc caccagtaaa acgacggttt
     2641 ctaaagacaa tccaacggtc tttgactttc ctttcaatgg agaagataac cggatgcatc
     2701 gggccgatgg ttggagcaat gggaatatgt ttaatacaac gtggcgggcc tctcaagtaa
     2761 acttcgaaga tggtaaaatg caactttcga ttaataaaga cgaggaaaat acaaaaattc
     2821 cgtatattag tggggaatac cgttccaatc aaaacttcca ttacggttta tacgaagtta
     2881 atatgaaacc aattaaaaat gatggggtag tgtcatcttt ctttacgtat accggcccaa
     2941 gtgaaaataa tccctgggat gaaattgata ttgagttttt aggtaaagat acgactggcg
     3001 tacaatttaa ttattttacg aatggcttgg gcaataaaga atactatcat aatttaggtt
     3061 tcgacgcctc agaagcattc catacgtatg cctttgaatg gttaccagat tcgatcacat
     3121 ggtttgtcga tggtgaaaaa atctataccg cgactgaaaa tattccgcaa acaccaggca
     3181 aaattatgat gaatgtttgg ccgggtactg gagttgactc atggttaaat ccctttgatg
     3241 gaacggtacc attaaaagcc gaatacgact ggattaaatt tacgcaatat aaaatcgttg
     3301 aggaagatgt cacgcctaaa ccagaagaac caacgacaga accaacgaca gaaccaacaa
     3361 cagaaccaac aacagaacca acaacagaac caacggaatc ttcaacaacg ggaagttcga
     3421 caacggaatc ttcaacaact aacagtagcg gaaatacgac accaccagca acaactgaaa
     3481 caactaacac aacctcaagc tcaactagtc agtcgtccaa agccgcaggc aaaacaacga
     3541 cgccaaaaaa taatagtaca actagtaaaa aagatttacc tgctacagga gagaatacac
     3601 atgctagcta ttggactgct tttggcggaa tgcttatttt agctggtcta ggcggcgcct
     3661 tattctttaa aaataaaaac cgtaaagcgt aatcggaggg gatttatgaa agcgaaaggt
     3721 gttatgctag tattaggctg tagcttattg cttggtttag taattaatcg cagtttggcc
     3781 catgagaaaa aaagtaaaaa tagtggcgag gagacggaga gtacgatggt agaaaataaa
     3841 aataacgcta aagctcagac aactgtcttt gactttccac tag
//
LOCUS       DAZKCN010000148         3880 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000148 DAZKCN010000000
VERSION     DAZKCN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.155"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..316
                     /locus_tag="ACG8K3_002567"
     CDS             155..316
                     /locus_tag="ACG8K3_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9146375.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="ACG8K3_002568"
     CDS             335..1612
                     /locus_tag="ACG8K3_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9146376.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="ACG8K3_002569"
     CDS             1622..2278
                     /locus_tag="ACG8K3_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146377.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="ACG8K3_002570"
     CDS             2278..3654
                     /locus_tag="ACG8K3_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9146378.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1548 a          413 c          675 g         1244 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt
//
LOCUS       DAZKCN010000149         3834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000149 DAZKCN010000000
VERSION     DAZKCN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.156"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..911
                     /locus_tag="ACG8K3_002571"
     CDS             <1..911
                     /locus_tag="ACG8K3_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFP9146379.1"
                     /translation="IENINISIRIENFYTLIFDNGKNLDEVYECIYKVVQFFYVLFGS
                     ELPVKFIEFEYGSINIGNKVIGKKYRMYVRQNAKVQSRKLRPYELFPYATIADNLSKY
                     INNWFAFYADLETIIQTYVGDLQLTSFLETQFLNACRNVEIFHRIYLEKEKIEGPEIV
                     FMRKKLMEIVTGAEEPVRKYFSERINYTGEETLSKRIKESLKVVPNPILKETILYRSK
                     SLSDSKTRFVRACVNTRNFLTHGSQDKSKYQPIFEKENLYMATKILNTIIRYFVLEKI
                     NIDSEILEEKILEDMNREGVSGNKLN"
     gene            1142..1378
                     /locus_tag="ACG8K3_002572"
     CDS             1142..1378
                     /locus_tag="ACG8K3_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9146380.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMMD
                     AIVKEESNLDLEIDMYVNQALSEYDSAFKELVDK"
     gene            1375..1788
                     /locus_tag="ACG8K3_002573"
     CDS             1375..1788
                     /locus_tag="ACG8K3_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9146381.1"
                     /translation="MIYLNEKDIVKLNVIQIKKYSPQETIGVIDKNALSMAVNQPKQH
                     IFGESLYPDEFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMENQEVV
                     EFVVRIATYQGEFDDLKEATTDILKNNSTKFDTKK"
     gene            2331..3257
                     /locus_tag="ACG8K3_002574"
     CDS             2331..3257
                     /locus_tag="ACG8K3_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFP9146382.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(3254..3832)
                     /locus_tag="ACG8K3_002575"
     CDS             complement(3254..3832)
                     /locus_tag="ACG8K3_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9146383.1"
                     /translation="MDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFVR
                     DQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKDL
                     IIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIVQ
                     DQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1422 a          514 c          658 g         1240 t
ORIGIN      
        1 caattgaaaa tatcaatatt agtattcgta ttgaaaattt ttatacatta atttttgata
       61 atggaaagaa tctagatgaa gtatatgaat gtatatataa agtagttcaa tttttttatg
      121 tgttatttgg ttctgaactc cctgtcaaat ttatagagtt tgaatatgga tcgataaata
      181 taggtaacaa agttattgga aaaaaatata ggatgtatgt caggcagaat gctaaagttc
      241 aatcaagaaa gcttagacct tatgagctat ttccttatgc cacaatagcc gataatttat
      301 caaaatatat taataattgg ttcgcatttt atgcagattt agagactatt atacagacat
      361 atgtaggaga cttacaactt acatcctttt tagaaacaca gtttctcaat gcttgtagaa
      421 atgtagaaat ttttcatcga atatatttgg agaaagaaaa gatagaaggc cctgaaattg
      481 tgtttatgag aaaaaaactc atggaaattg taacaggggc agaagagccc gtcagaaaat
      541 acttttctga aagaataaat tatacgggag aagaaacact ctcgaaaaga ataaaagaaa
      601 gcttaaaagt tgttccgaac cctattctaa aggaaactat cttatacaga tctaagtcat
      661 tatccgattc taaaactaga tttgtgagag cgtgcgtgaa tacaagaaat ttcttaacac
      721 atggcagtca ggataaaagc aaatatcaac ccatttttga aaaagaaaat ctatatatgg
      781 caacaaaaat tttaaatact attataagat attttgtact tgaaaaaata aatattgact
      841 cggaaattct tgaagaaaag atattagagg atatgaaccg tgaaggagtt tctgggaaca
      901 aattgaacta acaaatataa atctcacata attgaacttt tcttaaaaat ttgaactttc
      961 ttaatttaaa aagattaaaa tatttaatag acttatatat taataatctc aatgatagtt
     1021 gataaatttt agatagttcg tcctatattt gtaaaagata tacttaagca ttgcaatata
     1081 aatattaaga tattgattaa taaagatata cggtatatac ttgttttagg gagttgatta
     1141 tatgaatatt aaaaaaagga aaattagaga ggtaggcaac tcgatagttg tcactttgtc
     1201 aaaagaaagt ttattacaaa aaggattgaa acccggagat acaattttta ttgatgaaga
     1261 caaaatgatg gatgctattg tgaaagaaga aagcaattta gatcttgaaa ttgatatgta
     1321 tgtgaatcaa gctttatccg aatatgactc agcatttaag gaactggttg ataaatgatc
     1381 tatttaaatg aaaaagatat agtaaaatta aatgttattc aaataaagaa gtattcacct
     1441 caggaaacaa ttggtgttat tgataaaaat gctttgagta tggccgtaaa ccaacctaag
     1501 cagcatattt ttggagaatc cctatatcct gatgagttta gcaaagcagc agttctatta
     1561 ataaatctga tcaaaaaaca tccatttcat aatgcaaaca aacgtactgc atttctagct
     1621 gcatatgttt ttctcaagct aaatggttac tcactaaaaa tggaaaatca ggaagtcgtg
     1681 gaatttgtag ttaggattgc aacgtatcag ggtgagtttg atgacttaaa agaggcaaca
     1741 actgatatac taaaaaataa ttcaacaaag tttgatacaa aaaaatagaa gcaaactagg
     1801 agttcagtaa attctgaaat gttagtttgc tttctatttt agtttacata agatacatta
     1861 tgcgaagttc attattttaa aagcttaaat aataaacttt tttataatac caatttattt
     1921 atgtaaacca atgaccttaa tcgcttaagc aagcatgtta tgtaatactc tactctctcc
     1981 aaaactaatg actatctatt ttcaaagaag ctagaatgat tatctgatca aatgataatt
     2041 tatatttaat tcctggtcta ctgctcttct tttaatcaat taatgtctaa cgtactgtac
     2101 acgataaagt aggaagactt attttcaact tcggattcat ttttttaatt cggtgaaaat
     2161 cactaaatta agagtttatc caagttcatt ttagctgaga atacgcttga aaataatttt
     2221 gatgaatttt cctgaattaa aatttattaa cttgttgagt gatataattg taatgtatta
     2281 ccaaatagtt agcacttaga ttagtgatgg aaaggaatag tgagaacaag atgactggta
     2341 gaaatcccaa agtatttata tcatattcat gggagaataa ggaaaataaa gaatgggcta
     2401 gagaactagc agacaaatta cttgaaaacg gtattgatgc atatatagat cagtatgatt
     2461 taagtttagg tgatagatta cctcagttta tggagcagca aatatccgat tcagattatg
     2521 ttctaattat atgtacacca gagtataaaa aaaaggcaaa taatcgtaca agtggtgttg
     2581 ggtatgaagg tcatattatc tcagcagaat tgctttctgg aaaaaatgaa cgtaaattta
     2641 tcccaataat taaagaagga cgtgcaagcg aagtacttcc tacttttctt gctggaaagc
     2701 ttggaattga tttatcagat agccagcatt atgatgaaaa cttagatgat ttaattacaa
     2761 ctatatttgg tgttaagaga aaaccagcat taggtgatat acctaggcat attaaaggca
     2821 gtcctttact acctatagaa cataatgaaa ataaagatat tgaaatctta ggaattatca
     2881 ctgataaagt aactgtacct aaaatggatg gctcaagggg ttctgcatta tataaaattc
     2941 catttagatt aaatagagta ccaagtcagc tttggaaaga gttgtttgtt agaaattgga
     3001 attcaccacc acgatataca acaatgcata gaccaggaat tgcttctgtt gtagacgatg
     3061 aaattttact taatggaaca actattgagg aagtccggga atatcatcgg gaaactttaa
     3121 aactttgcat tgaaaaaagt aatactcaag aaaaaaaaat attgctagag gaggaacaaa
     3181 aaaaagagag agagaatgag cgagtcagat cacataaaga aagtgttcag aaattagcag
     3241 atgaattaga gttctagttg atagtcagtt ctttcttaat tcgatacaca gtttgtctag
     3301 ttacattata gtctgttgct atttttgaaa tagcaacacc attctcctgg tcctgaacga
     3361 tgcttctata aacaaggcga cgttgaggat cttttgtgtt cgcactgtat aacttaggac
     3421 gtcctttata gacgccgttt gctttagcaa tctttatccc ttgtgcctgg cgtcgttttg
     3481 attcagtacg ctcttgttca gcaatcatgg ccaagatttg aatgattagg tctttgataa
     3541 atttatccag gagaggattt ccgattgctt cagccatgat cggtaaatta gtgataataa
     3601 gtcgcacatt tttcttcttt agataattta ctgaatcgat aatctcgtca taattacgtc
     3661 caagtcgatc gatagcctct acaataaaaa tatcttggtc tctaacaaaa tctagaactt
     3721 cctgaaaaat cggtcgatga gtaatagtcg ctccagattg tttctcgatg aaaatttttt
     3781 cagcaccaaa ttttctcatt tcctcgattt gacgttgttc attttgatcg atcg
//
LOCUS       DAZKCN010000150         3785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000150 DAZKCN010000000
VERSION     DAZKCN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.157"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..168
                     /locus_tag="ACG8K3_002576"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACG8K3_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            406..642
                     /locus_tag="ACG8K3_002577"
     CDS             406..642
                     /locus_tag="ACG8K3_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146384.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="ACG8K3_002578"
     CDS             649..2601
                     /locus_tag="ACG8K3_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9146385.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8K3_002579"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8K3_002579"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9146386.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3445..3785
                     /locus_tag="ACG8K3_002580"
                     /pseudo
     CDS             3445..3785
                     /locus_tag="ACG8K3_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAZKCN010000151         3682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000151 DAZKCN010000000
VERSION     DAZKCN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.158"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..80)
                     /locus_tag="ACG8K3_002581"
     CDS             complement(<1..80)
                     /locus_tag="ACG8K3_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146387.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKG"
     gene            complement(82..1521)
                     /locus_tag="ACG8K3_002582"
     CDS             complement(82..1521)
                     /locus_tag="ACG8K3_002582"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9146388.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            1706..3652
                     /locus_tag="ACG8K3_002583"
     CDS             1706..3652
                     /locus_tag="ACG8K3_002583"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9146389.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1155 a          649 c          623 g         1255 t
ORIGIN      
        1 cccttattat taatgacgcg tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca
       61 tcagttaatt ttgctaccat tttaatattt taattcaaag tattttccag taggttcgcc
      121 tgatttgatt tgaataccat tttggtgatt ttcagggaat acacgactag taaacacttt
      181 ttctccttta ttaataaaaa tctcaatgat tgagttatct acaaaaatgt tagcagttgt
      241 ttctttagta tcaattgagc attcacgaat cgtaccaaag tcagtggcat attgaatgcc
      301 tgctttgctg cgatctaaaa taatttttcc ttctttggta tctacaatca agctaagtcc
      361 atttccttta ctatcagaga aaagaagtaa ctccgatttc tgatttggag gaaaagttag
      421 ttcaagctca taagtattat ttgtttctgc ttttgctgta aattgttcag aactagcacg
      481 taatgatgta atagcttcta ctggatattg gtaaagttta ccatctttta ttgaaagttc
      541 ttttacaaga ctcattgctc cctgataatc atacttatca gttggataat cgatatcagg
      601 tagtccaatc caactgacaa tcagagcacg accatcagga gtattgaatc cttgagtcgc
      661 atacgcttca aatccataat caagattttg aatttctgaa gcgcccacaa gttttacttt
      721 ctctatgtca agcgattgac agatcttaaa agtattcgga tagatattcc cataatttag
      781 ctcagattta tccaaccctt gaggacaata aatgagaaca ggttttttgt ctacaaatac
      841 tagatttgga cactcaatca tgtattctga accagtgccg ccaaaatcaa ggttgccaac
      901 ttcctcccat gtttccacgt tattgtcatc cgctttgtaa agtttgataa agcctgattt
      961 ttctaaattt tgtgctccaa caatcgcata taattgtcct tcataattaa acatttgtgg
     1021 atcacgaaaa tgatcagtta catcagcagg tttatgaata agaacattct cacctttcgt
     1081 aatgttgtag tctttatcca tccatgctcc gatttgaagt gtatcacgtt cccaattttc
     1141 atttcgaacg tttcctgtat agagtaagaa tagattcccc tctatttcat atgcagaacc
     1201 ggaataagcg ccgtggctat ctcgttttgt gtctggaaga agttctgctc ctgttacatg
     1261 aaaatgaact aaatcatctg attcattgtg tacccattct ttcaacccat gagcagctcc
     1321 aaatggccaa ttttgataaa aaagcgtata ttttccgtta aaaaaagaaa aaccattagg
     1381 atcgtttaac aatcctgttt taggttcgat atgatagcta gcacgccaag gtgatcgttt
     1441 tacattatcc ttaattttat ccatctcatc tttagtccaa tcagaataag cacgatatcg
     1501 tatttctcga ggtaagttca tattttactc cttcaattta tctttttaca actagtatgg
     1561 taaacgtttt ccgttgaaaa atcaatcatt taatttaaaa aaataaaata tatgacaaac
     1621 gtttgacata tttttaagat atgctaaaat aaatttgcat ttcagtgaac cgttttcacg
     1681 taaaaataaa aaaggagtga ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca
     1741 agcggtcgga aaagataacc ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt
     1801 actaaaagat gacacgaaag tgaatcaaaa agctttagac gaaaatccag atgtgaaagg
     1861 cacatttaaa attgatggac aatatcaagt gattatcggt gcaggtgatg ttaatttcgt
     1921 ttatgacgaa cttatcaaaa aaactggact ttcggaaatt tcaacagatg atttaaaaca
     1981 aattgctagt aaagataaaa aattcaaccc cattatggca ttaattaaat tattatcaga
     2041 tatttttgtt ccaattatac ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa
     2101 ctttttaaca gctgctggac tttttggacc aaaatcaatt gaagaaatgt atcctgcgat
     2161 tgaaggtatt tcctcaatga tccaactgat gtccgctgca ccattcatgt tcttaccaat
     2221 tttagtaggt atttcagctg ctaaacgttt tggagcaaac caatttcttg gtgctgcaat
     2281 tggtatgatc atgacaacac cagatcttgg tggctcaact gaatattgga atatttttgg
     2341 ttatcatgtt tcacaaacaa actatgctta tcaagttatt ccagttttag cagctgtttg
     2401 gatattatct atattagaaa aattctttca taaaaaattg ccatcatcta ttgactttac
     2461 attcacgcca ttattatcag taatgattac tggatttttg actttcacag ttattggccc
     2521 tgtaatgtta ttgttgtcta atggaattac agacattatt gtttggctat ataacactac
     2581 tggatttatt ggaatgggta tttttggagg aacatattca cttatagtca tgactggttt
     2641 acatcaatca tttccagcta ttgaaactca attactttca gcgtggacaa atggtatcgg
     2701 acatggagac tttgtctttg tagttgcatc tatggcaaac gttgctcaag gtgctgcaac
     2761 atttgccatt tggttcttaa ctaaaaattc taagacaaaa agtcttgctt cctctgcggg
     2821 tgtctcagca cttcttggaa ttacagagcc tgccttgttc ggagttaact taaaataccg
     2881 ctttccattc ttttgtgctt taattggttc aggaattgct acagcagtag caggattact
     2941 taaagtagtt gctgtttcac ttggttcagc aggtttcttg ggatttcttt caattaatgc
     3001 aacatctatc ccattttacg tattgtgtga attgattagc tttgtagttg cttttgcaat
     3061 tacttacttc tacggtaaaa caaaagctgc tggtatcttt aaagcagaag aaattgctga
     3121 acaaacacca gttaattctt caaaagtagc aacaacagat ccaatatcta ctgatgttga
     3181 atcaacacaa acaaaagttt catcagaaac aattgttagt cccatttctg gtaaagtgat
     3241 tagtctttct tcagtaaatg atccagtctt ttcatcagaa gctatgggaa aagggattgc
     3301 cattaaacca aatggaaaca cggtctattc tccagtagat ggtactattc aactcacgtt
     3361 tgaaactgga catgcttatg gtattaaatc aaataatggt gctgaagttc taattcatat
     3421 tggtattgat actgtatcaa tgggaggaaa aggatttaca caaaaagtta ctactaatca
     3481 aaaagttaga aaaggcgata ttcttggagc atttgaccgt tcaatgattg agaaagaagg
     3541 tcttgatgat acaacaatga ttattgtgac caatacttcg gattattcag aagtaaatat
     3601 agtagctgaa aatacagtca ctgagggtac tgatttactt gaaatcaaat aaatgtattt
     3661 ccctgagtaa agatggaatt tt
//
LOCUS       DAZKCN010000152         3456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000152 DAZKCN010000000
VERSION     DAZKCN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.159"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8K3_002584"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8K3_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP9146390.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8K3_002585"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8K3_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          983 a          762 c          539 g         1172 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataa
//
LOCUS       DAZKCN010000153         3219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000153 DAZKCN010000000
VERSION     DAZKCN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.160"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(439..1053)
                     /locus_tag="ACG8K3_002586"
     CDS             complement(439..1053)
                     /locus_tag="ACG8K3_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9146391.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1659..2036)
                     /locus_tag="ACG8K3_002587"
     CDS             complement(1659..2036)
                     /locus_tag="ACG8K3_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146392.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACG8K3_002588"
     CDS             complement(2336..3217)
                     /gene="galU"
                     /locus_tag="ACG8K3_002588"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9146393.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          978 a          659 c          498 g         1084 t
ORIGIN      
        1 tttgtgtatt ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg
       61 taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg
      121 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
      181 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      241 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      301 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      361 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      421 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      481 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      541 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      601 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      661 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      721 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      781 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      841 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      901 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      961 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
     1021 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
     1081 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1141 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1201 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1261 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1321 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1381 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1441 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1501 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1561 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1621 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1681 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1741 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1801 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1861 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1921 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1981 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     2041 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     2101 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2161 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2221 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2281 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2341 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2401 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2461 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2521 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2581 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2641 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2701 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2761 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2821 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2881 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2941 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     3001 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     3061 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3121 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3181 gcccagcggc aggaatgact gcttttttga ccttcactt
//
LOCUS       DAZKCN010000154         3201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000154 DAZKCN010000000
VERSION     DAZKCN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.161"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..363)
                     /locus_tag="ACG8K3_002589"
     CDS             complement(214..363)
                     /locus_tag="ACG8K3_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146394.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            825..1037
                     /locus_tag="ACG8K3_002590"
     CDS             825..1037
                     /locus_tag="ACG8K3_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146395.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(1315..3114)
                     /locus_tag="ACG8K3_002591"
     CDS             complement(1315..3114)
                     /locus_tag="ACG8K3_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP9146396.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         1134 a          559 c          562 g          946 t
ORIGIN      
        1 aatattatca tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga
       61 acttgtgttt ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga
      121 atacaaaaag acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga
      181 tctcatcagt cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac
      241 tgcttgttcg attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat
      301 aaaacggtta gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt
      361 cataaaattc atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg
      421 aacttatacc aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt
      481 tccttattta ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca
      541 atcatcaagg ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa
      601 taacttagca atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt
      661 tgtcacaacg tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa
      721 aataaaaaat aaaaaaatga ataaaaacgt tttcaaatac aaggtaattt gttattataa
      781 aataaagaaa aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag
      841 taggaagatc ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg
      901 aacgtacata tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc
      961 aacttatcaa tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcaccta
     1021 ctgaattggt ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa
     1081 taaagacaga agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat
     1141 tattcttgca gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga
     1201 atcaagagaa agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt
     1261 ggcaggttat gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc
     1321 gactcccaaa acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt
     1381 cacactttca tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat
     1441 catgcgactc atggcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg
     1501 gtgggcagaa ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc
     1561 aggcgtcaca gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg
     1621 ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt
     1681 ctctaagata actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt
     1741 tttagaggtc ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata
     1801 agaatcaaaa gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa
     1861 ttctttcaat gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc
     1921 atcatgcatc actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc
     1981 aggatgatag tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg
     2041 ggtactcaca cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata
     2101 atccatattg taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt
     2161 gtttttcagc gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc
     2221 aggcaagaag ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga
     2281 catcacgata ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat
     2341 caatgcatag tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata
     2401 tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc
     2461 caaaagacgc gatttggtca atgaccagct ttcccgaatg gctgctcgaa aagaacgatc
     2521 tcgtagaatc atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat
     2581 ataaacgagt aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat
     2641 gaaagcgggg atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg
     2701 tagaatcaat aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag
     2761 ctgaagtatc gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa
     2821 aaaaacactc atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa
     2881 aactacgatt aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc
     2941 ataagacaaa tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa
     3001 gggtaaacaa ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg
     3061 tgttcctttt aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc
     3121 acctgctctt cacattgtag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg
     3181 cagacccttt cccagattta a
//
LOCUS       DAZKCN010000155         3147 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000155 DAZKCN010000000
VERSION     DAZKCN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.162"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..568)
                     /locus_tag="ACG8K3_002592"
     CDS             complement(200..568)
                     /locus_tag="ACG8K3_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP9146397.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            718..1200
                     /locus_tag="ACG8K3_002593"
     CDS             718..1200
                     /locus_tag="ACG8K3_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP9146398.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1237..2247
                     /locus_tag="ACG8K3_002594"
     CDS             1237..2247
                     /locus_tag="ACG8K3_002594"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP9146399.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2362..3075)
                     /locus_tag="ACG8K3_002595"
     CDS             complement(2362..3075)
                     /locus_tag="ACG8K3_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146400.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1064 a          510 c          614 g          959 t
ORIGIN      
        1 aataatttta ctaagtggaa aattcaaagc cattcaatca gtgatttttt tcagcacaag
       61 aaagactcct tataaaaata gcagatactt tgtcaattaa gtgtttaacc agggatgata
      121 aaaaggatag gattgaacaa aacctgtata agctgatgaa tagtttcctc gccagtaaaa
      181 ggtttattcg tataaagctt tatcgccaaa atttatcatg acctttttta tcttcaatat
      241 taaatgaaat caattgttca atcaattccc agttgattac acttttccaa ggaactttga
      301 tgacgttatc cgtatatacg tagcctgctt cttggatatt tttttcaagg gcacgaatcg
      361 ctgctttttc cggtgcgata gaaaaatgat ttttagcaaa actaaatgca ataataaagg
      421 taccatggtc agtaaacata ggttggttcc attttacagt tgtgtctagc tggggatacg
      481 tttctgttat cttagcaaaa aggtctttga tcttttgttg atgttctttt tctttgatct
      541 gattgatgat cggctgaaat ttttccattc taaaacgcct ccattaagtg gtttgattta
      601 ttttaccaga aaaacaatag ttatgtataa aaaacgaaca aaacacaagt aatgttgtaa
      661 ccgctatatt tatatgataa gatgtctcaa ataataaaat agaaaagaga cataaaaatg
      721 attgacttgg caaagaaatt cttacacgaa aatatatatt ttgagttgta tcatcatcga
      781 gccatttata caaatgaaga tgctttggta ataaagaaag aacaaggatt tacaggtaca
      841 gagaccaaaa gcttattctt aaaagataaa aaagataata actacgtata tctgactttt
      901 actacgaaac aaacggattt caaagcatta gggaagatcg tcggtaaaag gctttctatt
      961 gtttcatcag aacgcatgga agaacaaacg ggacaaaagc agggcgctgt ttctccgttt
     1021 ggttatgaaa aacagattcc gattattatt gatgaagagc tcttatcaaa tgaaaaactt
     1081 gtttttgcac caggtagacc agatcaaacg atggtcatcc aaacggttga cttaaacaaa
     1141 atcatccagc tacttgagat gccgatattt ttgatgccgg caaacgaaaa aaacaattaa
     1201 ccaagtaaaa tagtgcatac ttggaggtga agcaacatga taagttatcc attattagaa
     1261 agaaatgcaa cgattggggt tactgcacca tcctcaggcg tatcagagga acaccattcg
     1321 ttactcgaat cagctttaaa gagaatggaa gaaaaatctt tccaagtagt tactggaaag
     1381 acgctttgga cgcaagataa ggaaaaatca ggtccagcaa gaaaacgagc agatgaattc
     1441 aatcaaatga tgcaagatga aaatatccaa atgatctttc cgccttgggg aggagaatta
     1501 ttgattgaga ttctcgattt tgtcgatttt gatgctctac aaccaaaatg gatagtaggt
     1561 tattcagata ttagtacgtt gctacttgct gtcacattaa aaacagggat agccactgca
     1621 catggaacca accttattga tttaagagga gagtacttgg atgaaacgac agcaatgtgg
     1681 gaaagtgtac tgaaaacaag agaaggagaa tcgattctac agcaaccttc tgaaaagtat
     1741 caaaaagagt ggccccatga gcattcatca tcttctgttt tccatctcac tgaaccaaca
     1801 aattggcaat cagtcagcgg agatgcagta aggatgaagg gacgattatt aggtggctgt
     1861 attgatacga tcagtcattt aatcggtacc ccttatggag atgtccaatc ttttaaaaga
     1921 aagtataatg atggaaagcc gatcttgtgg tttctagaaa attgtgagct atcgtcagtt
     1981 gatttaagaa gatcacttgt tcagatgaaa ctggcaggat ggtttgatgg cagctcgggg
     2041 atcatgttcg gtagaagtgc agcagaggat ttgttagatt ataaaatgaa ggatgtatat
     2101 gaagatctgt caaaggaatt aaaactaccg atcttgtatg atattgattg tggtcatgta
     2161 ccgccacaac tgacattgat caatggtgcc tatgcagaag tagcggtaga agatagaaaa
     2221 gcgactgttt tgcaaacgtt tatttaaaaa attttttcct gccttggagt ttatcaaaga
     2281 ccaacaaatc taaatagaaa agaaaatgaa agaagctgac agtattttgc cagcttcttt
     2341 ttgtatgatc tattagaaat tttaattatc cataactatc acattgatca gctgtttcac
     2401 atcatttgga ttacctgtag gaatagctac tacagatgca ttatcaataa tttcttgagt
     2461 tgtttctcta atgaacgctg gataagatgt ttgatctgca ggcatttgcc aatgtgaagg
     2521 aactggataa ctagtgattg tcccgtcatg gttacttgaa tacactaaaa ctccctggta
     2581 actgtatttc ccagtgagca taaccacatc tttcggatag actgcactcc ctgcatcata
     2641 aggggcgata ggtgttcctg caggataatg aataacatgt agttcgaaat cacccgatgt
     2701 atctctaaaa gtattacctc taattgctag ccaaacacgt gcgtactcaa tttgtagatc
     2761 agaatatcct gctaacggat ctattgactg agaagactgt gaggaagttg tgtctattgt
     2821 tgaagaagaa atatttgtct caactgtact actagatgta gaagaagtat tgggctctga
     2881 gactttactg cttgttgaag atgtattcgt ttcagtagaa gctgatttct gagtacttga
     2941 tttttcagct gaactggttt gtttgattga acttttgtct gtggtcgcag atggatcact
     3001 attttcttta ctaggagacg tacatcctac aaggaataaa gagaccaaca ataaaataaa
     3061 acctaacttt ttcatataaa atacctcatt atttttttaa taccagtata gcataatcct
     3121 gaatttttga atccgtaaaa ccttgtc
//
LOCUS       DAZKCN010000156         3114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000156 DAZKCN010000000
VERSION     DAZKCN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.163"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..324)
                     /locus_tag="ACG8K3_002596"
     CDS             complement(<1..324)
                     /locus_tag="ACG8K3_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9146401.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDE"
     gene            485..1174
                     /locus_tag="ACG8K3_002597"
     CDS             485..1174
                     /locus_tag="ACG8K3_002597"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9146402.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1188..2690
                     /locus_tag="ACG8K3_002598"
     CDS             1188..2690
                     /locus_tag="ACG8K3_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9146403.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2703..>3114
                     /locus_tag="ACG8K3_002599"
     CDS             2703..>3114
                     /locus_tag="ACG8K3_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9146404.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQF"
BASE COUNT         1011 a          441 c          584 g         1078 t
ORIGIN      
        1 ttcgtctaaa agcctaatag tggaatagag agcttctgtt aaactattag caatttcatc
       61 atagtacttt acactagtcg gatgttcttt tttttgacta aagtcttcct ttgcaatttc
      121 aatttttaaa tcagaaaaac cagaaaaacc aattttttga caaaagcgaa taatagtagc
      181 atcgccaact tttgtgtttt tcttaatatc actcatcgta ctataaataa tagttttccc
      241 agaatttaaa ataaaatcag cgactttttt ttcagacttc gttaaaaatg gataattttc
      301 tttaattaaa tagatataac tcatacaaac tcctctacaa tttcaacttt tctccattat
      361 actccaaaat aataaaaaaa actcctaaat actccatttt tatctttact tttatgaaag
      421 cggatgctat attgtttttg gagttattat ccaaaaataa taaaaaattg gagcgtttag
      481 gataatggat aaaaagaggt ttttagatct tatttcaaaa ggtttgattg tttcatgtca
      541 agcattacct ggtgaaccat tttacaaaga ggaagaaagt ttaatgcctt attttgcttt
      601 ggctgctcaa agggctggag cagttggtat acgtgcaaat tctgttcgag atattattga
      661 aattagaaag tgtgtatcat taccaatgat tggaattatt aaaagagatt atccaccaga
      721 atctccattc attactgcca ctatgagaga agtagatgaa ttagtagaaa ccggtgttga
      781 ggtaatcgct cttgattgca ctcttagaaa tcgacatgat ggactatcta taaattcttt
      841 tattactaaa ataaaagaaa aatatcctca tcagttattt atggcagata tttctacatt
      901 tgaagaaggc aaaaatgcat ataaagcagg aatagatttt gtaggaacta ctttaagtgg
      961 atatactgag gaaagtaaaa aacaagatgg gccagatata gatttgatta aaaaattggt
     1021 agaagaaggg atttgtgtaa ttgctgaggg aaggattcat actccagaac aagcaaaaga
     1081 gataaaaaaa ataggagttg caggtattgt tgtgggtggt gctattactc gaccacagga
     1141 aattgcatca agatttgtta aagcattaag ttagggggag aaataaaatg tttaaaaaat
     1201 tttcacaatt aggacgcgct ttcatgttac caattgctat attaccagta gccggattac
     1261 tattgggact gggaggagcg ttaacaaatg agagtgctat aaatgcttat ccatttttag
     1321 atcaaccgtg gttgcataca attttaagta ttatgagtta tgctggaaat gcagtttttg
     1381 caaacctagc actaattttt gcaattggta ttgctgtagg acttgcaaat ggagataaag
     1441 gaacagctgg tttagctggt ggagtagctt atttagtata tactgcaact attagtgggt
     1501 ttcttgaact tttttcagca aaagatgcaa cactagatac aggtgtagtg ggatcaattg
     1561 ttgttggtgg tactgttgca tttttacaca atcgttatag aaaaattgaa ttaccacaat
     1621 tcttaggttt ttttggagga tcaagattta ttccgattgt ttctagttta gctgcaatca
     1681 ttattggttc tttcttttat ttaatttggc caccaattca aaatggttta gtagttgctg
     1741 gtgaacaaat tgcccaaatg ggtagtttag gaactttttt atatggtttt ttattgcgtt
     1801 taacaggtgc tgttggacta caccacacca tttacccttt gttctggtat acatctttag
     1861 gtggtaccga aactgtagct ggatcaacaa ttgctggagc ccaaaatatt ttctttgccc
     1921 aactagctga tccaaaccac actggtctgt ttacttatgg tactagattt tttgcaggaa
     1981 gatttgcaac aatgatgttt gggttaccag cagcatgtta tgcaatgtat cgtgcaattc
     2041 ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag cggggccctt acatcatttt
     2101 taactggaat tactgaacca gttgaatata tgtttttatt tgttgctcct tggctttatg
     2161 tgattcatgc attcttagat ggattatctt tttattttgc tgatatctta aatattcgtg
     2221 tagggaattc cttttcaggt ggtttgatcg actttttatt attcggtgta cttcaaggga
     2281 atgacaaaac taattggtta aaattaattc cttttggtat tgcatgggca tttatatatt
     2341 tctttgtttt tacattcttt atcaaaaaat ttaaggtagc tattcctgga atggaagaag
     2401 aatcagaagt tcattctgaa aaagtaataa acaaaaatac atcattacat gaagagtctc
     2461 ttatcattat tgaagctctt ggtggaaaag aaaatattga gtctgttgaa gcttgtgcaa
     2521 ctcgtttgcg tgttgctgtt acgaatgggg agattattca aaaacaaaca ataaaagaca
     2581 ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca agctgttttt ggagggaaag
     2641 ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa agataattag gaggttttta
     2701 atatgtttga aattttcaaa aagaaaaaac aagaaacttt ttatgcacct tgttccggaa
     2761 aaatcatttc catcacagaa gtaaatgatc ctgtcttttc acaaaaatta ctaggtgatg
     2821 gttttgccgt catacctgat ggttcaaaaa tttatgctcc attggctggt cgagtaacaa
     2881 atatatttcc tactaaacat gctattaata taaaaagcaa ctcaggcatt gattatttga
     2941 ttcatattgg aattgataca gttgaattac aaggagagcc tttttcgatt tgtgttcaag
     3001 aaaatgatga ggttactcct gaaacctcgt tggttgatgt ggattttgac aaaataataa
     3061 aatctggtaa agatacttct gttatagttg tttttccgga gaaaaatcag ttca
//
LOCUS       DAZKCN010000157         3032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000157 DAZKCN010000000
VERSION     DAZKCN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.164"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            582..1541
                     /locus_tag="ACG8K3_002600"
     CDS             582..1541
                     /locus_tag="ACG8K3_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9146405.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYCF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1528..2133)
                     /locus_tag="ACG8K3_002601"
     CDS             complement(1528..2133)
                     /locus_tag="ACG8K3_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146406.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2373..>3032)
                     /locus_tag="ACG8K3_002602"
     CDS             complement(2373..>3032)
                     /locus_tag="ACG8K3_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9146407.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          988 a          503 c          471 g         1070 t
ORIGIN      
        1 taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat tagatatgaa
       61 aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag atgagagtca
      121 ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg atataggaag
      181 tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat ttttttgtta
      241 tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta tatcttctaa
      301 attagaagat atttgtatat caatatggaa tatattacct tttttgtatt tttttatgtt
      361 atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag ctggatttag
      421 tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt tttttatttt
      481 tttaccttcg ccgattgtaa aattaagcta gacaactaaa aaagtcattt gtgctacact
      541 caaaatagtt ccgtcgaaag aattataagg agtgagtaca aatgacctat acccatctta
      601 caccaactga actcgtaatg atagaagctt atttcaatca atcacactct gtttcaaaag
      661 tagcccatct ggtacaacgt tctcgacaaa ccatttataa tgtctattgt tttcttaaat
      721 ctggtggaag cgcaataagc tactatgaac aatataagaa aaacaaacgt aactgcggaa
      781 gacgtccgat tgtgttacca gatgggcaac aagaatacat tcaaaaaaag gtggctcaag
      841 ggtggactcc agatgtcctt attggtcgag cagagtttcc tattgcttgt tcggtacgta
      901 cactatatcg gatgtttaaa gataaaacct ttgaccctca cgatttacca atgaaaggga
      961 aaagaaaacc aaacggacat aaagaaaaaa gaggaaaaca agcttttcgc cgttcgattc
     1021 aggatagaac aactgattat atacagttca atcatgagtt tggtcaccta gaaggtgaca
     1081 ccattgtcgg tggaaaacat aaaagtgcgg tgatcacgtt agttgagcga ttatctaaag
     1141 tgatcatcac cttgaaaccc gctggtaggc aagctataga tattgagaat agactgaatc
     1201 aatggtttca atctgtaccg aaacacttgt ttaaatccat cacatttgat tgtggaaaag
     1261 agttctcgaa ttggaaaaag attagtaata ccaatgatat ttcgatttat ttcgctgatc
     1321 caggaacacc ttctcaacga ggactaaatg aacattcgaa tggcttatta cgtaaagatg
     1381 gcttatataa agctatggat tttaataagg tatccgaaac ctttatccaa tcggtggcat
     1441 caaaacgaaa tcatattcct agaaaatcat tacactatcg cacaccattg gaagtatttt
     1501 tgagttacgt aggtaaagat gatttgtcta gcttaatttg acaaacgaaa ctttgtaaaa
     1561 atattcgatt gaatattcta tttggcacga ctcacttggc agtatatgta tgtcaatatt
     1621 tttcatctgt ttcataggta tttcaaaatc taaactatta aaaacatata attccctgta
     1681 attatttaca tctaactcta ctaaatttat ctttggaaca atagatcctc cacccattcc
     1741 accataataa aaaaaatctt gttctctatc aaaatagcac atattaagct cagtttgttt
     1801 atcatcctca ataattttat aaatcaaacc tatataatca tgaatgctgg caaaaatttt
     1861 ttttaagtca agttctaaat cttcttcgaa aacacattta atttctccta ctgataattg
     1921 atactctata ttacatgact taattagttt tttagatccc ccctccagat aggcatatgt
     1981 ttcaataatt atgttctttt tattgcttgg caaatttccg ttatttattg cataaattaa
     2041 aaatgactgt ctatttagcc cataatctaa tacaacttct tcatactcat atttctttaa
     2101 atatttaata tgaaaatatg ctgaattaat caaaactcga gtgttactgt tgttatttat
     2161 tgttgttcta ttgtgtataa aattgtatag gttgtcatat tttcttggtt tagtgaacta
     2221 ctcaccactt agctaaacct aacggtttct tacgtttgaa gtgggagctt cttgggaaga
     2281 gagcatactt gatagcgtat tactttacta acagaggtgc ctcttattta ccaagctatc
     2341 cccgtagttc ctacggttct tgtttatttg atctaagcca atcccataga gtgtagcctt
     2401 agaccttcgg tcaggatatt gatactagcg ttgatatctc ggtcatgatg agtatgacaa
     2461 atagggcaag tccattttcg aatttcgaga gatttcttgc cgtctttatg tccacaattc
     2521 gaacagattt ggctagatgg aaaccactta tctactttga tgattttccg accgtaccag
     2581 tcagccttgt attgtaattt agacacaaaa ctagaccaag aaacatccga aatacttttt
     2641 gctagtttat gattacgcaa catgcctttt gtgtttaagt cttcaataca gatgatatcg
     2701 tgatttttga taatttctgt actcaactta ttcagaaaat cagtacgttg attcattact
     2761 ttttcatgca atcgtgctac tttacgtttt tgtgtttgat aatttctagc ttcaaacaga
     2821 ttgatacctt tttgtttagc taataaggca cgtcttgaca acttacgttg ttcacgtttg
     2881 agtttctttt ccattttgga cgtaaattta ttattatcaa atttttgacc ggtagaaaga
     2941 attgcaaagt ctataatacc taggtcaata ccaacagcag aattagattt aggtagttcg
     3001 cgaacttctt ctttgcataa caaagaaata ta
//
LOCUS       DAZKCN010000158         2909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000158 DAZKCN010000000
VERSION     DAZKCN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.165"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8K3_002603"
     CDS             complement(<1..461)
                     /locus_tag="ACG8K3_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP9146408.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(501..1553)
                     /locus_tag="ACG8K3_002604"
     CDS             complement(501..1553)
                     /locus_tag="ACG8K3_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9146409.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1566..2504)
                     /locus_tag="ACG8K3_002605"
     CDS             complement(1566..2504)
                     /locus_tag="ACG8K3_002605"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9146410.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          984 a          620 c          450 g          855 t
ORIGIN      
        1 ctttgacgat aaagcagaac aagtaacaat gggaaaagta gaaagcctgc atagcctagc
       61 ccagaacctg tattatggat ccaagtcgat agattccaag agctttcatt cgtattgata
      121 ctgaataacc cagtaaaaat acaatcacct atcccatata aaccaactgt tgcaacagca
      181 agtattgcaa gtgtttttga tgttccaaca aataggtgat acaacgcagg caacgaaaga
      241 acaaaaaaaa gtccagatac aactgaccag accaagaaag cacgtctgac tggactatct
      301 acgtcaccaa acacactaat tacggtagtc atttgattca gtttgggata aaaaaggcca
      361 aggatatacg gtgtcgataa atcactaatc acacctaata ataaaaaata aaaaccatat
      421 tttggcaaaa aaacgtaagt cagatgttgt tgacatctca cttacgcatc cattgattga
      481 aaatttctta gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg atcatacagt
      541 ttaaatactt tctcgcataa aaattgagta attagtgccg caaaaacagg gatgactaac
      601 caacaaagaa caacaagaaa gccatttagc cctgcatcta aagaagccaa cggaccaaca
      661 agtccgacca caccgaaacc agcagaagca ggcgtacctg aaatagaaaa tagagcgaca
      721 ggaatagctg taatcactgc actaactaaa caaggaataa gaatcacagg ttttctaaat
      781 agattaggca tcatcatctt catagcgcct aaagcaatcg caattgtcac acctgactta
      841 ttaaccttcc aagagttcac tactaaaaca aaagtggttg cagctacgcc catcgcagcc
      901 gcaccagcag aaacaccatc tagttggatt gccattccga ttgctactgt tgaaatagga
      961 gagataatca aaaaggcaaa ggaacaacca atcaagatag acattaacag cggttgtaag
     1021 gtagtgaagt tattaatcaa tgatccaatt gcagaagtaa ttgctgttac aaaagggaat
     1081 aaataaaaac caagtaaacc tgcacctaca cctacaacaa tcggtgttaa cacaatcgac
     1141 actgacccaa aactatttcc aatcagtaaa atcagcccga cagcaatcga tgctgtcagc
     1201 atcgtattaa taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc acccaaatca
     1261 ggtgtgaatt tgactacacc agaaccaaca aaagaagctc cggctacaac catcattctc
     1321 atcggatcaa atccaaattg taaggcaatc aaaccaccaa tgatcaaagg tgttgctaat
     1381 tgaaaaataa ctgctaactg tgcgatcgtt accgcaaagg gatacgcact aaaatatttt
     1441 aaaatagcac ctaatactgc attaggaatc aatccaataa tgattcctgt tgctgtacca
     1501 gcgaggactt tattcagaaa aattttcggt gatatttttt gtgaagtttc cattcaatct
     1561 ctcctttatt ttacgttcaa actatcttct tttgcatgga caagctcagt caagaaatca
     1621 caataaggcg tcgcaacacc atatttcttc ccttttctag aaattgcgcc attgatataa
     1681 tcgatttctg ttaatcgatg gttcttaatc aagtcttgat acattgaagg ataatgtaac
     1741 ccaattgttt ccgggtcaaa acaagattca caatgtgcaa tgacttctgg gacatctagt
     1801 tcaatcttct ctactgctgc tactttggca aactcattga caatcgtcgc caccatttta
     1861 tgagcagtcg atgtttttcc tagctcagcc atattaacgt ctaagattgt gcatagtccg
     1921 ttcattgttc cgttcacaca tgctttgcga taaatagaat agtgaatatt gtcagaaaaa
     1981 tgagcattta atcctgattc agacaatttg tctgctaatt tttttgcagc tgcttctttt
     2041 ccatcaccta aattttgtaa ttctactgaa ccacttccaa ataatttgac ctgaccagga
     2101 ccttcaagac ctgccgtcca catggtattt ccaatataga tattttccat tggtacaaat
     2161 ttctcaataa tatcttcatg cccgatacca tttaatagac ataagacctc tgtatctttt
     2221 ttgattaatg attggatatc ctgcagcatt ttttccagct gcatcgcttt ggtaaataga
     2281 ataatcagat caacttgatc ttctttttct acttcggatt gaagaacgat tggtagtttt
     2341 gcttctacct ctttaccatt aaaatttgct tgtaatccat gttctttgat ttgttggaca
     2401 tgttctgccc agccatcgat taataatact tcgttcccac tttggtgaag cattaaccca
     2461 aagcgacttc ccattgcgcc agcgcctgca attgctattt tcattgattc ttcctccttt
     2521 ttctttctat tatacttatt tgagaatcat ttcacaacct tgttttgtta ttcttctctt
     2581 atttatcatg tttatatcca ccattcaagt tattttatca gcaaatcaat aaaaactgga
     2641 atagaatccc aatttcatcg taagagaaac tagactagca ataagaaaat agaaacattc
     2701 atagaaaaaa cctcactcct ctgattataa aagaggggac attaacctcg tgtatactaa
     2761 aaccttccat tcattcgatg gatgattttc tttttgtgaa aaaatattca ttgtagtacg
     2821 atgttgcgca acacagacgg atggtattgc ttattattta ttaaaatcga acaatacact
     2881 tatttaaaaa cttctttccc agattttct
//
LOCUS       DAZKCN010000159         2905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000159 DAZKCN010000000
VERSION     DAZKCN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.166"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..549)
                     /locus_tag="ACG8K3_002606"
     CDS             complement(7..549)
                     /locus_tag="ACG8K3_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP9146411.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            684..1073
                     /locus_tag="ACG8K3_002607"
     CDS             684..1073
                     /locus_tag="ACG8K3_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146412.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1077..1841
                     /locus_tag="ACG8K3_002608"
     CDS             1077..1841
                     /locus_tag="ACG8K3_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9146413.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1970..>2905
                     /locus_tag="ACG8K3_002609"
     CDS             1970..>2905
                     /locus_tag="ACG8K3_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146414.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRE"
BASE COUNT          922 a          494 c          607 g          882 t
ORIGIN      
        1 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
       61 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      121 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      181 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      241 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      301 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      361 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      421 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      481 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      541 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      601 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      661 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      721 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      781 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      841 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      901 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
      961 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1021 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1081 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1141 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1201 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1261 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1321 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1381 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1441 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1501 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1561 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1621 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1681 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1741 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
     1801 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1861 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
     1921 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
     1981 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
     2041 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
     2101 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
     2161 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
     2221 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
     2281 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
     2341 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
     2401 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
     2461 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
     2521 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
     2581 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
     2641 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
     2701 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
     2761 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
     2821 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
     2881 ttttcctgaa ctagttatca gagag
//
LOCUS       DAZKCN010000160         2866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000160 DAZKCN010000000
VERSION     DAZKCN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.167"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..173
                     /locus_tag="ACG8K3_002610"
     CDS             <1..173
                     /locus_tag="ACG8K3_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP9146415.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
     gene            complement(263..1582)
                     /locus_tag="ACG8K3_002611"
     CDS             complement(263..1582)
                     /locus_tag="ACG8K3_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9146416.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1579..2232)
                     /locus_tag="ACG8K3_002612"
     CDS             complement(1579..2232)
                     /locus_tag="ACG8K3_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9146417.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2520..>2866
                     /locus_tag="ACG8K3_002613"
     CDS             2520..>2866
                     /locus_tag="ACG8K3_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9146418.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
BASE COUNT          964 a          455 c          297 g         1150 t
ORIGIN      
        1 tcaataagca aattaaaaga tacagccgta gaaaagagca gtttcaaaat gaagaatcac
       61 tagaacgctt tctagtcagc atttttgata catacaatca aaaatttcta aacagaagcc
      121 ataaaggttt tcaacaggta accgatacat tagtttcaat gtttactgag taactaatta
      181 ttttgcagga ggacaattta tttacacaaa attattgacg ctccccctat ttttcaataa
      241 ctattgcaaa attacatctt aattaagaaa atatttttat tttaaagata aaaaattctt
      301 catcctgcaa tactttatat tctatattgt aattattcag aatattactt acaatatata
      361 aacctaatcc attactattt tccttattta aatcaaattt aacatcaaat attttatcca
      421 tattcgaaat tttattgtta ccgtatgaat tctctatata taaccaatca ttaactatcc
      481 caatattaat taccccattt acatcagtat attttaccgc attactaatc aaattagata
      541 gaataatctt taaagctgtt tttcctatat aaacattctc gtctaatata taattattga
      601 cagttatttt cttttgattt gctaatattt catacttttt taatatgtct tctaatgtat
      661 catttatttt caaatattct tcatcattat tcaaattttc tatagaatga actgataata
      721 tttgagaaat attttttgtt aaactatcaa cgatttcaat acattcatta atataaatat
      781 ctctttcttt atattttcca atattgtatt tcatgttttc cagtattatt ttaagactag
      841 caagaggggt tttaagttca tgggatgcac ctttaaaaaa gtcatatttt aacttttcta
      901 attttaaaat ttctttattt ttaaattcca aatcgtcaat tgttcttaat aaagtagaat
      961 acaaatcatt aatttgttgt tttaattctc caacttcatc attagagcta acttttaaac
     1021 tcattttttt atcaagtttc atcattttat cagtaacaat ttttatttct tgtatattat
     1081 tttttattga ttttgcataa attaaagaaa taatagcaga aaataaaatt gatattaata
     1141 aagaatatgg taaaaattta aggcttaaat ctttcgcatc tttttgcata tcagctgtag
     1201 aaacaaattg tagatttgtt tttttaccat cattaagctt tatttctctt tcttcaatta
     1261 tcaaagaatt actatcactt tctaaattaa catttatatt atctttgatt tgtatttcat
     1321 tcttattatt tttttctttt atgaatactt ttatttcact actcttggaa taaaactcca
     1381 aagtttgttc tatatatttt atttcttttc cattcatatt actagaaatt tcatttgcca
     1441 tattatgaat cttttctttt ctcgtctcca aatatgtttt aggaaagata aaaaaaatta
     1501 atgaatgaac taatataatt attattccaa gaacagaaaa tatttgtatg aacatttttg
     1561 gaaatatctt taattttttc attttctctc caatttatat cctacatttc ttatagtagt
     1621 tatacagtct aattgtaatt tttttctaag ttcttttata tatacatcta ttactcgatc
     1681 ataaggagtt tcttcgctat ctttccatac ataatcaata atttgcattc ttgttaacac
     1741 ttgtccatta ttatccaata aacattttaa tacttctagt tcttttgcat ttacatctat
     1801 ttcttcacca tttattttag ctgtatagct attaaaatta accgatagat ccttatattc
     1861 aaacttctct aaatgtccat aattcttctt aattaaagaa tctattctag cttttaagac
     1921 cggcaatgaa aatggctttt ctacatatcc gtctactaaa ttagtaaatg catcaatttt
     1981 atattcttca tcactaaatg cagtcaagat tagaattggt aaattgcttt tctttctaat
     2041 ttctttcaac acttctaaac catttataaa aggtatctga atgtctaaga taaccaaatt
     2101 tatatcgcta ttaaattttg acaaggcttc tcttccatct ttagcttgaa taacagtata
     2161 tccaaattct gaaagatatt cacttattcc ctctcttatc atattatcat cttcaacagt
     2221 taatattttc atatatacct ccacttaagc ctatacacta gatattatac agcatattat
     2281 ggctctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt ctttttttta
     2341 ataaatgcaa acagacacct tgaaatcctt tagaataaag ggttctataa tattttgtgt
     2401 tgttggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga
     2461 gactcacttt tggtaaaatt tagtcgacca aaacatcacc ctaccaaagg agtcgctcta
     2521 tggataatca tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag
     2581 aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa gggagattgg
     2641 atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc aacggttcct
     2701 atacgacacg tactcagata ttgaaagtca aagaaaagtt gactatttta gaattaaaac
     2761 gtacccgttt tctatgctat gattgtgggc agaccttttc tgcgaaaacg gacttagttg
     2821 atgaacatca tcaactaaca aaggaactga agcaggcgat tctcat
//
LOCUS       DAZKCN010000161         2862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000161 DAZKCN010000000
VERSION     DAZKCN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.168"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..488)
                     /locus_tag="ACG8K3_002614"
     CDS             complement(126..488)
                     /locus_tag="ACG8K3_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146419.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(562..942)
                     /locus_tag="ACG8K3_002615"
     CDS             complement(562..942)
                     /locus_tag="ACG8K3_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP9146420.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1024..2199
                     /locus_tag="ACG8K3_002616"
     CDS             1024..2199
                     /locus_tag="ACG8K3_002616"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9146421.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2276..2860)
                     /locus_tag="ACG8K3_002617"
     CDS             complement(2276..2860)
                     /locus_tag="ACG8K3_002617"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9146422.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          891 a          554 c          482 g          935 t
ORIGIN      
        1 attttgaaaa taaaatgcac taaaaaacta tctttagcag aacatttcat tgtatttttt
       61 ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct
      121 gtattttaga agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga
      181 cctggttctt cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt
      241 tcaaagacaa acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa
      301 ctaatcttgc taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa
      361 ccagtctttc cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg
      421 accaaaaatt ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg
      481 cttgccaaaa tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca
      541 ttttcagaaa aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc
      601 ttcttctatc caaataggta actgctccgc aatagtggta aatccgatct tacgattttt
      661 attcgtgaaa tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct
      721 taaagataaa ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg
      781 ctgtccgact tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac
      841 atgaataagt ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc
      901 tgtcactttt ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc
      961 atttatcatt atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt
     1021 aagatgatcg aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac
     1081 gcaattgctg agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac
     1141 ttcttgtctc ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt
     1201 ggttatgcca ctctgtctga taaactatat caggctatca ttcattggga acgccaacac
     1261 catgctgtgg agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta
     1321 gctgcagcag tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt
     1381 tatcctccat tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg
     1441 ctagaaaaaa ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa
     1501 aacaatgtaa aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc
     1561 aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt
     1621 gacgagatcc atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt
     1681 gatcctaatt tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta
     1741 gctgcaatca aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa
     1801 aaacatttag tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa
     1861 gcggcttatg agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat
     1921 gttcaaacag ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct
     1981 gaaggaactt acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg
     2041 gagaacactc ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct
     2101 tccggtcatg ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga
     2161 cttaatcgaa tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag
     2221 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2281 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2341 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2401 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2461 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2521 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     2581 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     2641 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     2701 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     2761 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     2821 gtcctttttc actttttaaa tctagttgtg ggaatgccat at
//
LOCUS       DAZKCN010000162         2760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000162 DAZKCN010000000
VERSION     DAZKCN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.169"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1168)
                     /locus_tag="ACG8K3_002618"
     CDS             complement(74..1168)
                     /locus_tag="ACG8K3_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP9146423.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="ACG8K3_002619"
     CDS             1357..2118
                     /locus_tag="ACG8K3_002619"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9146424.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="ACG8K3_002620"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="ACG8K3_002620"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9146425.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          873 a          550 c          466 g          871 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
//
LOCUS       DAZKCN010000163         2473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000163 DAZKCN010000000
VERSION     DAZKCN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.170"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..252)
                     /locus_tag="ACG8K3_002621"
     CDS             complement(67..252)
                     /locus_tag="ACG8K3_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146426.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            467..838
                     /locus_tag="ACG8K3_002622"
     CDS             467..838
                     /locus_tag="ACG8K3_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP9146427.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(988..1060)
                     /locus_tag="ACG8K3_002623"
     tRNA            complement(988..1060)
                     /locus_tag="ACG8K3_002623"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Gln,seq:ctg)
     gene            complement(1106..1534)
                     /locus_tag="ACG8K3_002624"
     CDS             complement(1106..1534)
                     /locus_tag="ACG8K3_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146428.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1594..2352)
                     /locus_tag="ACG8K3_002625"
     CDS             complement(1594..2352)
                     /locus_tag="ACG8K3_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP9146429.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          773 a          413 c          455 g          832 t
ORIGIN      
        1 aagaaccaaa ggaggacgat gcagcagcat tgtcctcctt tgattttaca aaagtgaaag
       61 ttttacttat ttgaatagat ttttaaattt gtttttgaca ctgtcgatat ttacatcttt
      121 taaaagattt tgggctttct ctttataagg tcctaattgt tctttatgtt cttcgataaa
      181 tcgttttgcg ccatcgaaat tgccgttctt aatcatttcc ttgacttttt gaaataattc
      241 ttcttgattc attttgaaca catcctttgc tatcaacatg attttttaca tggacaaaac
      301 gagaaaaact gatttatcca cacgtcaata atatcaaata acgatcattt ttactaaaaa
      361 aacactcgat tctcgcttct aaagtaggag aaaaatctcc aacttgggac ggatggaatt
      421 tctttaactg acccatatac tgaatatata acaaggaggt agaaagatga aagtgattaa
      481 atatttgatt ggactggcgg cgattagtgg tattttgcta tttggactga aatattatac
      541 tgaaggaagt tatggagaaa tccctggtat acttgatcag ctaaatccat tagttgaaaa
      601 aggagaggtc tatgtaaaga cacaaaaacc agaggaagta aatgaatatg gtacagcaag
      661 gtatgtgcag aaagctgctg atgccaatgg gaaagaacga acggttgaat ttaatggtct
      721 ttctgtgtta aaagaaaatc attatttgaa aattagcaat aaaggagcac acatagaaac
      781 gtatgaggaa gttcctagag aaaaagttcc ggagaaagca ttagcaatta ttgggtaaat
      841 cagtttttgt attaagaata cgcccgattt tattattcat aatttaatta tgtaaactaa
      901 atacaagtag taaaaataat acaataatcc ccctgcaaaa agattgttct tttacagggg
      961 gttcattttt tcagcaagca catcaaattg ggatggcagg gttcgaacct acaattgctt
     1021 gattcagagt caagtgcctt accagtttgg ctacatccca tttattcaac aaaataatca
     1081 tagcactttc ctaaaaaaac gcaagttatt tttcttgatc ggatagttgt tgctttactc
     1141 gtaaaaggcg gcgtactagt ttcttttcta atccagcagg aagggcaatc agttctgcta
     1201 ctgacaagac ggtgtccaat aactgatatt ctttgacagg ttcattcata atatgataga
     1261 tgtttgctaa acgttcaata tattcaaagc gagtatactc tgttcgagca attagaaatt
     1321 tcaataaatc ggtggctttc tcgtactctt gatctgcgta ataaacaaga gattgacgat
     1381 acaatttgat ttccagttca ttgagggtag caggtctgcg acgctcgttc cactcattaa
     1441 tagagacttt accttgaaca tttcccttgc agtcacggac aaaaataatc ggaatttcat
     1501 ttggatccat ttttttatgt gtgaattttt ccattatcta cgctcccttc aaaggtttgg
     1561 tatcttgagt ataagggatt tttgtttgaa ttactagctt ttctccaaag ttagcatctg
     1621 tccacccatt gcagtgaaaa tcgccgaaaa atcgttgatc ggtactgctc gattttttac
     1681 accataggga tcgttgacat aaatattttc tttgtcatat ccagtgacta cacaagcatg
     1741 aacaagagga gtgactctta cttctccatt atttgtggtc caagtcataa aatcagtttc
     1801 atctgggact tcaaaattga tagtcacaga agtccagaca ggatttcctt tagatagtgc
     1861 ttttttgata tcatcgagtt gagcatcggt ttttggaaaa gtgtgataat ccgtttggac
     1921 gatttgctga gcaagggagg caactggggg aacatagacg accatagccc agtctccgcc
     1981 actgatatca ccaacaaatc cttcattagg atcgccatga gtcccatctg cattgaaaac
     2041 agggacgtaa tccaattgct cagctaactg gtttttagtt gtttcataac cataaaaaga
     2101 taaaagcata gaaagcgaag tgacttcaca gccattttct aaagaaggag aatcaaactg
     2161 gctctctaat ggaacatcta gtaaaacttc tgctttctct tttgtctttt tcgttttttg
     2221 tgttgaactt ttttgtgcag tgttcattgt atgttctgga gaatggatat gtaggtaata
     2281 acttgtaccg cttaataaaa gagcagctgt gccccctaga aacaatagtt ttttgaacgt
     2341 ttttttcttc atttgaaatc tcccttttca gctgtttatc tttcctatta taaatgtatt
     2401 tttattggaa atggaagtat tatattttaa tatgtaggaa ataattagtt tttaaggaat
     2461 atgttttaat ttt
//
LOCUS       DAZKCN010000164         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000164 DAZKCN010000000
VERSION     DAZKCN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.171"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8K3_002626"
     CDS             207..2069
                     /locus_tag="ACG8K3_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9146430.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG8K3_002627"
     CDS             2069..>2400
                     /locus_tag="ACG8K3_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9146431.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKCN010000165         2313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000165 DAZKCN010000000
VERSION     DAZKCN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.173"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..973
                     /locus_tag="ACG8K3_002628"
     CDS             65..973
                     /locus_tag="ACG8K3_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFP9146432.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            1382..1468
                     /locus_tag="ACG8K3_002629"
                     /pseudo
     CDS             1382..1468
                     /locus_tag="ACG8K3_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(1626..>2313)
                     /locus_tag="ACG8K3_002630"
     CDS             complement(1626..>2313)
                     /locus_tag="ACG8K3_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146433.1"
                     /translation="LERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDR
                     FHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVW
                     RASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLP
                     ETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIM
                     IRHGKTFLTK"
BASE COUNT          780 a          369 c          415 g          749 t
ORIGIN      
        1 gttggtaaat caacaaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact
       61 acatatgcgt tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga
      121 aattatgaga actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg
      181 aagaaatact gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg
      241 cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt
      301 tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta
      361 cactatcaag attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg
      421 aattatagat agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact
      481 attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct
      541 taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc
      601 aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat
      661 cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc ttaagggcat
      721 tactatatcc gttccgccac gtaaaaacat ggataaatca gaaaaactag accacagctt
      781 acttggaaaa caaagaaaaa cagttgagac tgttttttct tccttagaaa aattaggttg
      841 tcaaaatttc aactcacgtt ctgttaaagg gctagagagt agatttgaaa gcatattact
      901 ggcttacagt gttctattaa gtcgagcaca acgacgtttt gaaggaactt tgagatattc
      961 tttaggatat taaaattaac tagcaccacg ggtacttgtt aattataaac taatttattt
     1021 aggtagtaca taattaacag ataaaactac atgcttgtag tttgtctagc taatgagttg
     1081 catatgattt atataaaggt gtgcagcata acttagttga agttaagaga atagaataaa
     1141 tattttttgt tttgctaata attaaacact acctaaatat ggtagtgttt aattatataa
     1201 tattcatttt gttacaaata atcgtattat aaaagcataa caattgggat aactattttt
     1261 tagattattt aagtactact taaattaggt agtacttaaa ttttggttta aggtagtagg
     1321 taattaagaa ataatatata tcttctttta taatagaagg ggagaggcct agttaaaagc
     1381 agcgacaatt aataacaatg tatttaatgc ttcacaaaat ccatttggtg gacaatgcat
     1441 tgttattagc gatgatctac cgttctaagt atttttttct attggttgca ttcgtgacta
     1501 attatggtat aattatgagt aacaaagagg tatttcatta ggctctttgt caaatgatgt
     1561 tggtaaagag aaatcttaga ataatttgga cagaacagaa agaagaaaac ctctttctgt
     1621 tctgtttatt tggttaaaaa tgttttgcca tgacgaatca taattcgtag tttgaaattg
     1681 taaaagttgc gaaagccgta cgcattgcgt tttaatactt tgatgtggtt gtttaagcat
     1741 tccaatggtc cgtttgaata gctgtagtga agcgcatttt ggatctgtgg cagaaacttt
     1801 ttaaacgttc gtaaagtggt tgtataggtt tctggcagac cgctaatgtc tctagttaaa
     1861 caatcctcaa atagtgactg atctcttttc tttaccgcat aaagaatgtc ttggtataac
     1921 tgatagcctt ggcgtaagtt gggacaagca gctaacagtc gatcggcaat ctcactttca
     1981 gtcaaataat ctttaaaact ggcacgccaa acccgttttt catagtttaa ctttgcttct
     2041 tctttttgta ggagtttcca atagcgcttc aattttttcc ctaaccctcg ttgagagtgg
     2101 gaatttcctt taagtcgtac cgtggtttct ttcactcgat gattcaagaa agttctgcca
     2161 atatgttgga cgatgtggaa acgatcgatg atcaactgag cattcgggaa aagctttttc
     2221 accaacgaaa cataaggcgc atacatatca ataacaatca attgaacggc ttcacgagtc
     2281 gctaatgaaa accgagaaaa atagcgttct aaa
//
LOCUS       DAZKCN010000166         2310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000166 DAZKCN010000000
VERSION     DAZKCN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.174"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..655
                     /locus_tag="ACG8K3_002631"
     CDS             221..655
                     /locus_tag="ACG8K3_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP9146434.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            678..998
                     /locus_tag="ACG8K3_002632"
     CDS             678..998
                     /locus_tag="ACG8K3_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146435.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1139..1330
                     /locus_tag="ACG8K3_002633"
     CDS             1139..1330
                     /locus_tag="ACG8K3_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146436.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1401..1658)
                     /locus_tag="ACG8K3_002634"
     CDS             complement(1401..1658)
                     /locus_tag="ACG8K3_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP9146437.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            1816..2079
                     /locus_tag="ACG8K3_002635"
     CDS             1816..2079
                     /locus_tag="ACG8K3_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146438.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2103..2291
                     /locus_tag="ACG8K3_002636"
     CDS             2103..2291
                     /locus_tag="ACG8K3_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146439.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          899 a          323 c          477 g          611 t
ORIGIN      
        1 cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa
       61 tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta taaataactt
      121 gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt ttgtattttt
      181 gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa gataccagaa
      241 catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg aagcccttga
      301 cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca aagttgagct
      361 gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt
      421 agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga tccacgcaat
      481 agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac aagaaaatat
      541 tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat tagttggatc
      601 gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga
      661 taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag
      721 aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg atcgctttag
      781 taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac caacaagata
      841 aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg cctgataaat
      901 ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa gcattagata
      961 aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc aatcagcctt
     1021 tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga
     1081 ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat
     1141 ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa aatatgaaga
     1201 agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca
     1261 agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt tttttggtat
     1321 aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt aatcgttttc
     1381 tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat ccaaataata
     1441 agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga cgttgtttcc
     1501 caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga ggccagtaaa
     1561 actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat cataataaaa
     1621 aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc gataaataaa
     1681 gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc
     1741 ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca aagtgaaaga
     1801 aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt gatcaataat
     1861 attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa
     1921 ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga aggaagttat
     1981 tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga agatctagat
     2041 accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga tggaggaaaa
     2101 aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc gaaaagattg
     2161 atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg gagaatcgta
     2221 aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac gatccgacac
     2281 gtttatttta gtatgaagta ttcagaaaag
//
LOCUS       DAZKCN010000167         2271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000167 DAZKCN010000000
VERSION     DAZKCN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.175"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8K3_002637"
     CDS             complement(<1..300)
                     /locus_tag="ACG8K3_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP9146440.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8K3_002638"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8K3_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8K3_002639"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8K3_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146441.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="ACG8K3_002640"
     CDS             complement(1512..2204)
                     /locus_tag="ACG8K3_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9146442.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          648 a          432 c          333 g          858 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag c
//
LOCUS       DAZKCN010000168         2165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000168 DAZKCN010000000
VERSION     DAZKCN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.176"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1057
                     /gene="gap"
                     /locus_tag="ACG8K3_002641"
     CDS             47..1057
                     /gene="gap"
                     /locus_tag="ACG8K3_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9146443.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(1142..2077)
                     /locus_tag="ACG8K3_002642"
     CDS             complement(1142..2077)
                     /locus_tag="ACG8K3_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9146444.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          639 a          462 c          393 g          671 t
ORIGIN      
        1 gaggatgccg tttcacagca cccctaataa aggaggaact tttgatatga cagtaaaagt
       61 aggtattaac ggatttgggc gtattggccg tctggctttc cgccgcatca aagaagtctc
      121 agatgatatt gaagtagtag caatcaacga cttaacaagt ccaactatgt tagcacaatt
      181 attgcaattt gactctactc atggtactta tcctggcaca gttactgcaa cagaagactc
      241 aatcgtagtg gatggcgaag caactcgtgt ttatgcagaa cccgacgcaa gcaaaatccc
      301 ttgggcaaaa gaaaacggtg tagatatcgt actagaatgt actggttttt atacatctga
      361 agaaaaagca aaagcgcatt tagatgctgg tgtcaaacga gtagttattt ctgccccagc
      421 tggtgctatg aaaaccatcg tctataacgt caatgatgat acattagatg aaaacgacaa
      481 aatcatctca gcaggttcat gtacaacaaa ctgtttagca cctatggctt atttcttaaa
      541 taatgaattt gggatcgaag ttggtacaat gacaacagtt catgcctaca cttccacaca
      601 aatgctctta gatggacctg taaaaggcgg taatttgcgt gctgctcgtt cagctgctga
      661 caatactatc ccacattcta ctggtgctgc gaaagcaatc ggcttagtta tccctgagct
      721 acaaggaaaa ttacaaggac acgcacaacg tgttccagtc gttgatggtt cattaactga
      781 attggtttca gtattgaaaa caaaagtaac tgctgatcaa gtaaacgaag caatcaaaaa
      841 acacacagtt gacaatcctt cattcgggta tgatgaccgt caaatcgttt ctagcgatgt
      901 cattggaaca actcaaggtt cgatctttga cccaacgcaa acagaagtaa caactgctgg
      961 tgatttccaa cttgttaaaa ctgttgcatg gtatgacaat gagtatggat ttacttgcca
     1021 aatgattcgc ttactagaaa aattcgctaa cttgtaattt gatagatcaa tcgataaaag
     1081 aagcctttga ctggatccag tcaaaggctt ctttcttttc atcccctact tttaaactcg
     1141 ttcaatgaac atattggctt aaaaattgtt ggactacttg ctgatatttt tcaggatctg
     1201 tcttgtatgc ttccgcatgc tcggctcctt ttacaacata cttttctttt ggtcccttcg
     1261 ttgcacgata gaccttatct aacatttcaa atggtacaaa tgtatcttcg tctccatgta
     1321 taaagagcat tgggcgctgg tttttcttca attgttccac tgcatttgct tcaccgaaaa
     1381 agtagccagc acggactttt gtcactagac tcgttacttg cataagcgga aatgctggaa
     1441 gactaaacat atcgtctagc tgataagcaa gctcatcatg aacggaagaa taaccgcaat
     1501 cttcaatgat tgctttcaca ttatctggta gtttttctcc gctagtcatc ataactgttg
     1561 ctgctcccat gctgacaccg tatagaacga tttcttgcga ttttccattg ttttctagca
     1621 ctttgtttat ccactgtaca tagtctttac gctctggcca tccaaatcca atgtaatccc
     1681 cttcgctttt tccatgtcct cttgcatcag gaacaaggac attgtaaccc atatcatgaa
     1741 acatctttgc gtaattggtc atcgtttcag cgtttcccat atagccatga gccacaagag
     1801 ctgttttttc acttttcgtt tcagcaggaa gataaatcgc tgataatttc agaccgtctt
     1861 ttgaagtgat ggtccaatcg cttcggtttt cttttgaact aaaccattta tgtgcatccg
     1921 tttctttgga tgtctgtgtt gtaccaggtg tatcttcgtc taaaaagtct ttctcagaag
     1981 gcacaacagc ataattataa aaataattcc ctgcaaacac taacgcaaca atcagagcta
     2041 aagcgacgcc taaaaggatt ttcatccaaa gtttcataaa tcatccatct catttctctt
     2101 ttttcatata accttgaatt agtataatca aagaatctct actatgcaat gttcattttt
     2161 ataac
//
LOCUS       DAZKCN010000169         2125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000169 DAZKCN010000000
VERSION     DAZKCN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.177"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8K3_002643"
     CDS             <1..159
                     /locus_tag="ACG8K3_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFP9146445.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8K3_002644"
     CDS             242..859
                     /locus_tag="ACG8K3_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146446.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACG8K3_002645"
     CDS             1023..1355
                     /locus_tag="ACG8K3_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146447.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACG8K3_002646"
     CDS             1679..1840
                     /locus_tag="ACG8K3_002646"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFP9146448.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACG8K3_002647"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACG8K3_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAZKCN010000170         2107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000170 DAZKCN010000000
VERSION     DAZKCN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.178"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1114)
                     /locus_tag="ACG8K3_002648"
     CDS             complement(83..1114)
                     /locus_tag="ACG8K3_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146449.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1121..1480)
                     /locus_tag="ACG8K3_002649"
     CDS             complement(1121..1480)
                     /locus_tag="ACG8K3_002649"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9146450.1"
                     /translation="MDAAETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYML
                     TNLLIGGRFQTVRIYMLRRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSL
                     KQTREEKQSKKKPLKEL"
     gene            complement(1559..>2107)
                     /locus_tag="ACG8K3_002650"
     CDS             complement(1559..>2107)
                     /locus_tag="ACG8K3_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP9146451.1"
                     /translation="TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYL
                     YRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVKRFDEPKVVVTDKAPSITSAFKKLKE
                     YGGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTR
                     KEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          601 a          507 c          373 g          626 t
ORIGIN      
        1 aaatcaattc taccttagat gttcttatat cactctatca gtcctatttg acaaagagcc
       61 agaaatgttt gccgactata cactagcttt gttgtgcaaa atgctgagta tcaatttcca
      121 cagggattgc tagaatatct ttcagctgaa tgtataaggc atcgctaatt ttctcttccc
      181 gatcgttcaa ccgatccact aaaatttctt tgccctccag cgtttgaaaa acataccgtt
      241 gaatgttgcc taaaataaat gattccttcc atagaaccgg aatcttaacc ccctgaggaa
      301 ccggttcaaa ttgaatcagc tccggtctta aataataaat gcagcgattg cccttagcgg
      361 aaaactcgtg ggtatactga ttcagctcat accattcgaa acgattatag ttgccgataa
      421 attccgcaac aaatcggctc tgaggattct gatacaattc cttcgaggtg gaaacctgag
      481 ccacccgccc agcttccatc acaaaaatcc gatcactgat tcgcatcgcc tcttcttggt
      541 catgagtcac aaaaatcatc gtcatcccta gcgattgctg gatagcgcgc aaatcccgct
      601 gcagctgttt tctaattcgg gcatccaaag cacttaatgg ctcatccagc agcaggactt
      661 tcggttcagt gaccaatgag cgagccagtg ccacccgttg tcgttgaccg ccagaaagaa
      721 actggacatt tttatctgcc tgctccgtca tatccaccaa tgatagcatc tcaaaaacct
      781 tttcctgaat cacctcagga ctcagcttct ttaccttcaa accaaaggct acattttcaa
      841 aaacagtcat agttggaaac aatgcatact gttgaaaaac aaagccgata ttgcgatcct
      901 tcgtaggctt atcggcaata ttctcattgt ccagcacaat ctcaccgctc tttggttgtt
      961 ccaatcccgt gatacagcgc agcaacgtag actttccgca cccgctggga cctagtaaag
     1021 taaccagctc acccttttca atctgaattt catcaatagc taaaatctgt ttttcatcaa
     1081 aaaacagatt gagatcatgt atttctaaaa acattttccg tcacaactcc tttaagggct
     1141 tctttttcga ctgcttctct tccctcgtct gcttaaggga cagctgttgc ttttgtcgat
     1201 aagtcagcat aaaggtagtt aaagcaattc ccaccataaa cagcaaatac aaaaccatca
     1261 cagcgctagc caaatggccg ttttcgttca tccgccgcag catatagatg cgtaccgtct
     1321 gaaatcgacc gccaatcaac aggttcgtca gcatgtactc cccaaaaacc gaagaaaaaa
     1381 tcatcagaga aactagggtc acaccgatac ggatgtttgg aaacagcacc tggataataa
     1441 tcgtagacat cggggcgccc aaggtttctg ctgcatccac taattccttc agattaatta
     1501 agcgggttct gttgcaaagt tttaaataaa gaataaaatc ccttacggta tctatgattt
     1561 aagctgggat tcccaataat accttgattt cagtacagac cgaaaacccg aagagagtgc
     1621 cttcttttcg ggttttctta tataatcctc ggatggcttc catgccttta atcgtggtag
     1681 aggcagtgcg taaacttcga tagaatttat tgcgtctctt tactgggcga tggtcttgtt
     1741 caatcaaatt attcaggtat ttaatggtac gatgttctgt cccttgataa aagccgccgt
     1801 attcttttag tttcttaaag gcacttgtaa tagagggggc tttatctgtg actacaacct
     1861 tcggttcatc aaaccgcttc actaaccgct taagaaaagc atacgctgct tgtgtgtccc
     1921 gttttttacg taaccaaata tccaaggtta aaccatctgc atcgatggct cgatacaaat
     1981 aatgccattt tcctttaatt ttgatgtacg tttcatccat tttccatgaa taaaaggatt
     2041 ttttattttt ctttttccaa atttgataaa gtagtttgcc atattcttgc acccaacgat
     2101 aaattgt
//
LOCUS       DAZKCN010000171         2051 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000171 DAZKCN010000000
VERSION     DAZKCN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.179"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2051
                     /gene="acm"
                     /locus_tag="ACG8K3_002651"
     CDS             <1..>2051
                     /gene="acm"
                     /locus_tag="ACG8K3_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP9146452.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     L"
BASE COUNT          804 a          338 c          404 g          505 t
ORIGIN      
        1 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
       61 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      121 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      181 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      241 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      301 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      361 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      421 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      481 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      541 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      601 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      661 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      721 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      781 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
      841 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
      901 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
      961 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1021 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1081 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1141 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1201 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1261 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1321 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1381 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1441 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1501 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1561 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1621 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1681 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1741 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1801 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     1861 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     1921 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     1981 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2041 tggtttattg a
//
LOCUS       DAZKCN010000172         1916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000172 DAZKCN010000000
VERSION     DAZKCN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.183"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..398
                     /locus_tag="ACG8K3_002652"
     CDS             6..398
                     /locus_tag="ACG8K3_002652"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146453.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="ACG8K3_002653"
     CDS             399..1838
                     /locus_tag="ACG8K3_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFP9146454.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          807 a          138 c          325 g          646 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat gatggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagt
//
LOCUS       DAZKCN010000173         1909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000173 DAZKCN010000000
VERSION     DAZKCN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.184"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..208
                     /locus_tag="ACG8K3_002654"
     CDS             <1..208
                     /locus_tag="ACG8K3_002654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146455.1"
                     /translation="IISPNPISVQADGVFLRFYIIELTGRLTVFFGNFAIKPFAEVTA
                     HYICKWLDCKNKIKKVFHYISSSP"
     gene            486..710
                     /locus_tag="ACG8K3_002655"
                     /pseudo
     CDS             486..710
                     /locus_tag="ACG8K3_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            970..1224
                     /locus_tag="ACG8K3_002656"
     CDS             970..1224
                     /locus_tag="ACG8K3_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9146456.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1225..1542
                     /locus_tag="ACG8K3_002657"
     CDS             1225..1542
                     /locus_tag="ACG8K3_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9146457.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          661 a          300 c          355 g          593 t
ORIGIN      
        1 cattatatca ccaaacccca tcagcgtgca ggctgatggg gtttttttac gtttctatat
       61 tatagaatta acgggtaggc ttactgtctt ttttggcaat tttgcaatca agccatttgc
      121 agaggtaact gcacactaca tctgcaaatg gcttgattgc aaaaacaaaa tcaaaaaggt
      181 atttcattat atctcctcct ccccttgaca gattaggccg gggtagcaga cagcaaaact
      241 attataacat aactgaaata cctaaatgac atatacagat atgataattt atgtattgat
      301 ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt atatattttt
      361 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctaaatagag
      421 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
      481 atgaaattgc cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
      541 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
      601 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
      661 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
      721 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
      781 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
      841 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      901 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      961 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
     1021 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
     1081 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
     1141 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
     1201 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
     1261 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct gtctccagcc
     1321 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     1381 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     1441 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     1501 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     1561 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     1621 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     1681 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     1741 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1801 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1861 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttg
//
LOCUS       DAZKCN010000174         1886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000174 DAZKCN010000000
VERSION     DAZKCN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.185"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1098)
                     /gene="queA"
                     /locus_tag="ACG8K3_002658"
     CDS             complement(67..1098)
                     /gene="queA"
                     /locus_tag="ACG8K3_002658"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9146458.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1291..>1886
                     /locus_tag="ACG8K3_002659"
     CDS             1291..>1886
                     /locus_tag="ACG8K3_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9146459.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT          541 a          394 c          350 g          601 t
ORIGIN      
        1 tttttctttg tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc
       61 ataaccttat ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat
      121 cgcatgctgg taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac
      181 taaagtcgat tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata
      241 gccaggcgta atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt
      301 cgttccaatc gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc
      361 atttttacgt acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact
      421 atgcatatgg tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac
      481 atgtagtgtc agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt
      541 cgtaaaatgc aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt
      601 ttgatagcga tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc
      661 caatgattca agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc
      721 gtgttccaat tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt
      781 cccaactttg gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt
      841 cgttagaaga aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc
      901 aggcagaact cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat
      961 ttcatggaaa tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga
     1021 atgatcgcgg tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa
     1081 atcttctgtt gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat
     1141 gtttttcgtt ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat
     1201 aaaaaaacta ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc
     1261 ttcattgaaa atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt
     1321 tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc
     1381 atttgtttta tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga
     1441 atgactgatc caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat
     1501 ccagtggaat tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac
     1561 atgacgatcc gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa
     1621 cggaataaaa cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat
     1681 cgctacccac atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt
     1741 gcagcgaatc aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact
     1801 cgtgattcct tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta
     1861 tttgtaaccc atgacatgga tgaagc
//
LOCUS       DAZKCN010000175         1859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000175 DAZKCN010000000
VERSION     DAZKCN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.186"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(486..785)
                     /locus_tag="ACG8K3_002660"
     CDS             complement(486..785)
                     /locus_tag="ACG8K3_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9146460.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            1136..1768
                     /locus_tag="ACG8K3_002661"
     CDS             1136..1768
                     /locus_tag="ACG8K3_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9146461.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          647 a          298 c          224 g          690 t
ORIGIN      
        1 ctcccctatt ttaactaaaa ataaaaccta ctatatcatc tctcaaaaat cctttcttca
       61 attcctcccc cactcatttc caatctttta actaaaaaat tgatagttat tttacaaata
      121 tttataaaaa ataaatatgc caaaaaactg tttttacaag gtttttccta atgtaaatta
      181 ttttttcgca tatattcatt ttattttact tattgctttc attttttcaa tctatatttc
      241 caatttttaa cttacaaaaa tgcaacatta ataaataacg atagtttcat aaaatacact
      301 tttaattaac attattagtt tatactagct gtttttgtgt taattaaagg ataaaataca
      361 atataaagaa acatctgttt ctttatttag aataaaaaaa tacagactct gttcaaataa
      421 atgaacctag cctgcattat gagattgact atacttttta attcattgag atgaataact
      481 cgtttttata aatgcccaaa agtaatcggt gccgaagcca ttcctcgacc taattctttt
      541 tcaaattttc ctattgttga tatctccata taaaacggtg atactggttt agacaattct
      601 cccctgattg ctaacggacg taaactacgt tccaaattat ttatgacttt aggataatac
      661 tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta tacgttcctt ttctttgatc
      721 tctggattta aaacgagatc atataatttt agtaccatgt tttctatttc ttcactttta
      781 gacatcacga tttcctccca aaaatatatt tcttcccact acattctata aacaaagtat
      841 acgctacatc tagatttttt taaatcatta ctggattgtt aaaacctttc aatacactgt
      901 tatggcaacg atttttattt taaaaacatt ctaaattaaa tcagtaaaaa tttttattct
      961 aagattttta ttagaattac gcctaatttg tgtctctcag ggcaattcta agaacgtttc
     1021 aagagatagc gacactatcg ttatcgaaaa aggcgaaaac ctatctcatt ttctgctaat
     1081 gtaagttggt ttagcttttg ttaatatgac tttatgaaaa tggaggtaat taaatatgat
     1141 ttttcctaaa gttaatgaag caagcttgaa taaaggatcc ttttttcata tctatgaaaa
     1201 cgaagatttt gaactgtata atatcctttc acaaaaagag gtcatcgaaa accaaataat
     1261 ctgccatcca attatttaca aatctatttt caagtatgca ttttttgctt ccaatgattt
     1321 tcaaagaatg gagattaccg actgtatatt cgaaaactgt gatttttcag gctgtatatt
     1381 gacaaaaagc atgttgcatc gcgtgaaatt tgtcaattgt aaattaacag gtactaaatt
     1441 gatggaaagt tatattggga acacgttatt cgagaattgt aaaatggact atgtaaattt
     1501 atctggttca aacataaaag aaagtacctt tgagcactct gtcttatcaa gcgcagattt
     1561 tgtagactgt tccctcacta aaataatgtt ttctactaat gatttagaaa atgctagctt
     1621 ttttgatact gatttaaaaa atatcgattt aagtagcaac tcctttgaaa tgattgaagt
     1681 acgctcggaa catctaaaaa attgtacgct taatcagttg caagcacttg gttttgctag
     1741 aaaattttta caaataaaag tagtataatc aaaatgaata tataagataa atttttagca
     1801 atacaaatat ccataaaaaa acattgccta ttttaactct tcatgtatac tgactctat
//
LOCUS       DAZKCN010000176         1831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000176 DAZKCN010000000
VERSION     DAZKCN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.187"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..614)
                     /locus_tag="ACG8K3_002662"
     CDS             complement(<1..614)
                     /locus_tag="ACG8K3_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146462.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            655..1347
                     /locus_tag="ACG8K3_002663"
     CDS             655..1347
                     /locus_tag="ACG8K3_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9146463.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          559 a          345 c          328 g          599 t
ORIGIN      
        1 acttgccaag gattaaatga aaacgtactt ggcacgacgt gatctggatc ataggtctcg
       61 atgatttttt cgtattgttc cttgtagtca ttatcaactt ttgcttgggg gatcacttta
      121 cttgcttcat gcaaaaagtg ttctgaacca cgccccattg cctgtccccg aacagtatgt
      181 ttatccaacg caatatcagg aatttctgga atatatccca tagcgaaact cttgataaca
      241 atgttcttaa gtaaatccga tgaacgatct ttttcagatt ggcataggta acgaatcgcg
      301 tggaaaaaat acatcgcacg atcggattca ttgtattgga attctttgcg catggcgttt
      361 aagttattga tcatgatggc ggccatagga ttgcccatgc ccacatcctc aacactgatc
      421 gccaatagcc ttctccagag tttctcttca aattgtggtg aggtgatata catttcataa
      481 gcaaattcac atgcttcttt ttcttttcct cgacgaatcg atttctgtaa tgctgagata
      541 gcttcatctc cacgtaagcc gttacgggta gtcatttttg accacggatc ttgaataaat
      601 ttcgtttctg tcataatata atactcctat tagataaaat ttgaggtgaa tcttatggat
      661 gttgaaatgc ttatagataa ataccaactg aatgactcag aagctgaagt cttacgattt
      721 atgaaaaaaa atcgtcagtc tttaaaaaat attgggattc gtgacgttgc caaagcatcg
      781 tatgtttcta ctgcaacgat tatcaatatg gcgaagaaaa ttggatattc cggttatagt
      841 gagttggtat ttgctctagg aaacagccaa tctgacatta cgatgccaag cacattctcc
      901 aatgaggaga aggaagcatt catacattta atgtctgcgt atcgcgataa acggattatg
      961 attcttggct caggtttttc gcaaaatcta gccaattatt tttcggaatc tcttaatcta
     1021 tatggatttc gtgcgacggc caatagtcat ttagaatttc ttagacagca agtgcaacaa
     1081 gatattctgc tttttattat ttccaattct ggtgatacag ttcgattagc cgaattagct
     1141 aaaattgcga acgatcactt tatcgatgtg atcgcatttg ttgggcaaaa aaattcaaaa
     1201 atcggtacgc tggcaacatt aaccattagc acggaaacct atattccaca aaaaatatct
     1261 gattaccaac caaatctttt ctttggcacg acactgaatc aatttgagct tttgttgagc
     1321 gcggcgctga aggctattta taaatagaag aacgctgtgg ccactgtttc ttcaaaatct
     1381 agtataaaga atcttcttta gaatgtaatt gtttcctgtg tgttctgaac atgtttagga
     1441 atttccaaac ttgtttagaa aactttctca ttccctccgc tgtagaattg aactcacata
     1501 aaaaacatct aaaccaaaat atctacaagc ttggaaagga taataggttg ttattaaaaa
     1561 taatttttat tcctgaacct gaataattca tagctttaat tctttggcac attttattga
     1621 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
     1681 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
     1741 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactgcctc
     1801 gcctcaggcg agcaaagacc ctgtagaatt c
//
LOCUS       DAZKCN010000177         1813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000177 DAZKCN010000000
VERSION     DAZKCN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.188"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..606
                     /locus_tag="ACG8K3_002664"
     CDS             289..606
                     /locus_tag="ACG8K3_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP9146464.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="ACG8K3_002665"
     CDS             complement(697..1062)
                     /locus_tag="ACG8K3_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146465.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="ACG8K3_002666"
     CDS             1270..1752
                     /locus_tag="ACG8K3_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146466.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
BASE COUNT          610 a          251 c          307 g          645 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc ttt
//
LOCUS       DAZKCN010000178         1773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000178 DAZKCN010000000
VERSION     DAZKCN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.189"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..797
                     /locus_tag="ACG8K3_002667"
     CDS             201..797
                     /locus_tag="ACG8K3_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9146467.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(855..1337)
                     /locus_tag="ACG8K3_002668"
     CDS             complement(855..1337)
                     /locus_tag="ACG8K3_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146468.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1443..1640)
                     /locus_tag="ACG8K3_002669"
     CDS             complement(1443..1640)
                     /locus_tag="ACG8K3_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146469.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          596 a          360 c          271 g          546 t
ORIGIN      
        1 agcaaaaatt cctaaaacac aacaatgaaa gcgataacta agaaaatcct tacataacga
       61 tttttcttat taaaattgtt ttttgaactt cgcttatttt ttgtgagcat atctgtttac
      121 ttactttttg taaggtgcta tacttcattt cgtaatcaat taaaaaaatg caaaacgcaa
      181 atcaaaggag aaattttatt atgacaactt ttactgacac tttgaaaaac cgccgttcaa
      241 tctatcatct aggacgtaat gtttcacttt ctaatgaaga attaacaact ttgatcaaag
      301 aagcaattaa agaaagccca actgctttca atgctcaatc aacacgcgca gttatccttt
      361 tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga agcacttcgc ccattgactc
      421 cagcagaagc tttcccaaat acacaaaaca aattagctgg tttcaaaaat ggttatggta
      481 cagtattatt cttcaaagat actgacgtgg taaaaggtct acaagaacaa tttgaattat
      541 atgcagacaa cttcccagat tggtcagaac aatcaaacgg aatcgcaaca gctaatactt
      601 gggtagcatt agttgataaa ggattgggcg caaacttgca acattacaac ccagttattg
      661 acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg gaaactacgt tcgcaattag
      721 tattcggttc accagaaaca ccggctgggg aaaaagaata catgaacgat gccgatcgct
      781 tccgcgtatt tggataattt tatttgaaat aaaaaacaga ggttgtgaca aacatcttgt
      841 cgcaacctct gtttttattc tggatcaatc ataatatcaa tctctgcctc atcgtttaag
      901 cgttgggaca aaaatggaac taattcttct aatgatgcag aaaatttcag catcggtcct
      961 cgccatccgt ttcggaaaaa attcattttt acgattttag aaccttttgg ctgatataat
     1021 acaattttat tgatctcaga aaaaggaaca ccttttttgt caaagctatt caaaacaagt
     1081 ccttcttctg tgattcctct actgtccaac aaaaaactaa taaaaatcat tgctgacaag
     1141 atcccttttg ccaattcatc tgctgtagga tgactgatga gtgtatacca aatgactagt
     1201 gacactaaga agggtatcac caagtagacc catcgatttc gcacagtccg ataaataatt
     1261 tcttttcgaa ccagccaaag aaataaaaac atcacaacaa cgaataaaaa caatagaacc
     1321 gtaaagaatg ttaccatgac aactcgcctc gctttttctt ctccagaaaa taggaaaaga
     1381 atactgattt accttctcaa agaaattgga cttgcagcac gccgcacgtc caattgtttt
     1441 ctttattcaa atgtatcata ttttgcaaca accatgcttg ctttttcatc taataattct
     1501 tggtcctctg cagcacattg ggcaactttg accacggcgc agtttgtcat ttttgataca
     1561 acttcaccaa tgaccgtttt tgtaaaacca attggtttgc atgcatattg ttctcctaaa
     1621 ttaatcattt cttccgtcat caaaatcctc ctaaactgct ggaacttttc tccacattac
     1681 cactgctatt tctttggaga tttaactcat tgatttttat ttacagttaa aaatcaacct
     1741 tacaggcatt atagcgtact gttttcgttt tca
//
LOCUS       DAZKCN010000179         1754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000179 DAZKCN010000000
VERSION     DAZKCN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.190"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..231)
                     /locus_tag="ACG8K3_002670"
                     /pseudo
     CDS             complement(148..231)
                     /locus_tag="ACG8K3_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            580..1665
                     /gene="xerS"
                     /locus_tag="ACG8K3_002671"
     CDS             580..1665
                     /gene="xerS"
                     /locus_tag="ACG8K3_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP9146470.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          648 a          299 c          246 g          561 t
ORIGIN      
        1 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
       61 ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg gcttttttat
      121 ttgtctcgct taattataca aacggcgatc taaaaaaatt gtatctccag gatgcaaccc
      181 attttcttta agaaattcct tttaaattgt taatactact gagtgcccca cttctctgaa
      241 ttttctttaa cttctccctc ccctttttta tttgtatata cattcatata cgcttgttaa
      301 tatcaaatca accgtagtct ctcttatgtc taattatata gaaagattgt tctttctata
      361 taatttagac aatttaaaaa attaagattg cctccttctg tttcctttcc tattcatgta
      421 taccatttat tttaaattat taaatctata cctatttatc tgcttttttg aaataaatta
      481 ataaatttca aagtatacca attgacaaaa cactctataa ttggtatagt ccaattatag
      541 agatactcct ctcccctact tatcgaaagg atgaataaca tgaaaaatac agaaatatat
      601 caaaaaatag atacaatcat acttcatatg cccgattata taaaagattt tgttcaagat
      661 cgtgaggata aagatcaatc acctcgaact actttagaat atctaaaaaa ttataagctc
      721 ttttatgaat ggcttctgag tgagtctatc gttcccgata cttcaatcac ctcgataaga
      781 cagttaactg cctatgatct aaacttgtac aaatctcatt tgaaacgtcg cacaaaggaa
      841 aatgtaaaaa aagagacaac aaaaactaag ctcaaagata ataataactt aggtctatct
      901 acttctacta tcaatagaaa tatcacagca ttaaaggttc tatttaaata tctttcgaaa
      961 agttccaata atcctttagg aaaaccatac ttagaaaata atcctatgga tcaagtagca
     1021 actatcacag ataaaactac gttagctgcc cgtgcaaacg caatcgagaa aaaattattt
     1081 ctagacgaag atacacaaaa ttatctagat tatattgcca atgaatataa aaaaactttg
     1141 agcaaacgag ctttgatcta ttatcaacga gatgtagaaa gagacctggc gatcaatgca
     1201 ttgattttag gatctggatt aagactctcc gaggttgtaa atattaattt agaggaccta
     1261 tctttagaaa agaataatgt ggtagtaact agaaaaggaa ataagagaga cgcagtaaat
     1321 atagcagcat ttgcaatgga atatttagct aattatctag ctattcggaa agaacgatac
     1381 aaagtaacag aaaacgaaaa agctttattt ctagcaattt atcaaggaga agccaagcgt
     1441 atatccggta ttgcaatcga acggatggta gcaaaatatt caaaaggatt cagagtccaa
     1501 gtcagtccac ataaattacg tcatactttg gctactagac tctatcaaca gacaaattca
     1561 ttggtattaa ctgcccaaca attgggacat acaagtacaa atacaacaac tttatatact
     1621 catatagaca atgcagctac aattgatgct ttgaacgctt tgtaaatgta gtaaaaaata
     1681 gtataataat gaagcctgtc tctaaaatta gataaaagcc caactttaaa ggcaagcttc
     1741 attatcattt ttat
//
LOCUS       DAZKCN010000180         1753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000180 DAZKCN010000000
VERSION     DAZKCN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.191"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..687
                     /locus_tag="ACG8K3_002672"
     CDS             85..687
                     /locus_tag="ACG8K3_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9146471.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQNKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRQRCGISVADRPLD"
     gene            760..1149
                     /locus_tag="ACG8K3_002673"
     CDS             760..1149
                     /locus_tag="ACG8K3_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146472.1"
                     /translation="MKKLFANEKIDLLYSIILVLIWIIFLLISKLLHFHSEWVISFIG
                     VFVMGCINLPTILRRKKQYKKIDELRKVLNLSIKEVREIADIGRYDLSDWNWDKAHIS
                     PKKLYLLEDTLEKMYVKQFGKEFEMRK"
     gene            1322..1648
                     /locus_tag="ACG8K3_002674"
     CDS             1322..1648
                     /locus_tag="ACG8K3_002674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146473.1"
                     /translation="MALSQVCMVSFLQRFLGSTYRNYFIVVFWLLLSLIAFIVFVFNF
                     ESLWQDIAYWIVPSKDLYINKPEIIRDLIKVVPMIILGIVVFVIGIRQGLKNYYGDYS
                     GDTKQQ"
BASE COUNT          618 a          223 c          364 g          548 t
ORIGIN      
        1 tttctgataa gcaatttgaa attgaaaaat tagatatatc tgattggtta ttgcaaccga
       61 gagaatttta gaggttggtt gaaaatggcg aaaattggtt atgcacgtgt cagtagcaaa
      121 gaacagaact tagatcggca attgcaagcg ttacagggcg tttctaaggt cttttcagac
      181 aaattaagcg gtcaatcggt cgaacgtcca caattacaag ctatgcttaa ctatattcgt
      241 gaaggggata ttgttgttgt tactgaatta gaccgcttag gacgaaacaa caaagaatta
      301 acagaactga tgaatcaaat tcaaaacaaa ggcgcaaccc tggaggttct aaatttaccg
      361 tcaatgaatg ggattgaaga tgaaaattta agacggctga ttaataattt agtgattgaa
      421 ttgtataagt accaagcgga atctgaacgc aaacgaatta aagaacgcca agcccaagga
      481 attgaaattg ctaagaaaaa aggaaaattc aaagggcgac aacctaaatt caaagaaaat
      541 gatccacgtc tacaacacgc ttttgatttg tttttgaacg gtttatctga taaagaagtt
      601 gaagaacaaa ctggaatcaa tcggcgaacg tttagaagat accgacagcg gtgtggcatt
      661 tcagtagctg atagaccttt agattaatat gaacgtggtg aagataacat aaaagagaaa
      721 acaagaaagt aatagtaata gaaatgaggg gtagacatta tgaagaaatt gtttgcaaat
      781 gagaagatag accttttata ttcaattatt ttagttttaa tttggattat tttcctactt
      841 atcagtaagt tgcttcattt tcactctgaa tgggttattt cttttatcgg agtgtttgtt
      901 atgggatgca tcaacttacc aacgatttta cgaaggaaaa aacagtataa aaaaatagat
      961 gaactaagaa aagtgttaaa cctatccatt aaagaagtaa gggaaattgc cgacattggt
     1021 agatatgatt taagtgattg gaactgggat aaagcgcata tttctccaaa aaaattatac
     1081 ttactggagg acaccctaga aaaaatgtat gttaaacagt ttggaaaaga atttgaaatg
     1141 agaaagtagt tttttaagga aatattccaa gtggaggtat tcaaagctat tcaagatcga
     1201 aagattcttt tggttatatt tttgttggtt ctgattgtca gtgtggtgtt aatcattggt
     1261 agttttaaaa taaggaaaca aagtagggga acggctttat ttttgctttt attgggaata
     1321 gttggcatta tcgcaagtct gtatggtttc ttttttacaa cgttttttgg gatcaactta
     1381 taggaactat tttatcgttg tgttttggtt attgctgtct ttaattgcgt ttattgtttt
     1441 tgtatttaat tttgagagtt tatggcaaga tattgcttac tggattgttc caagcaaaga
     1501 cctttatata aacaaacctg aaatcataag agatttaatc aaggttgttc caatgattat
     1561 tttaggaatc gtagtttttg tcattggtat tagacaagga ctgaaaaatt actatggcga
     1621 ttattcaggt gacacgaagc aacaataact aaagctatct aaaattagga aaaaaggaga
     1681 gaatgaatga gtacagttat tttagctgaa aaaccaagcc aggcgttagc ctatgcccag
     1741 gcatttaatc aga
//
LOCUS       DAZKCN010000181         1689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000181 DAZKCN010000000
VERSION     DAZKCN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.192"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACG8K3_002675"
     CDS             <1..243
                     /locus_tag="ACG8K3_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146474.1"
                     /translation="FCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISI
                     INKFDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            269..880
                     /locus_tag="ACG8K3_002676"
     CDS             269..880
                     /locus_tag="ACG8K3_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP9146475.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            1062..1577
                     /locus_tag="ACG8K3_002677"
     CDS             1062..1577
                     /locus_tag="ACG8K3_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFP9146476.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
BASE COUNT          551 a          218 c          330 g          590 t
ORIGIN      
        1 ttctgcgtca tccattatat tttttataga aaagtttgca aagaactatt ggatggaaaa
       61 aacatatatg atgtcagagg aatacttttg atttctgcaa tcacgatagc tggtggcatc
      121 ctaatttcaa taattaataa atttgatttc ataaaatatt taattatagc aattgcttgt
      181 gtcgctgcaa ttatatttag aaaacgattg ctacttttag taaggagaat tatgagcaaa
      241 taaacatttg ctcatagaga gatagagaat gatagatatt atttctgcta taacggtaaa
      301 tgtattaaca gcattatatc agccgttctt atttgccgta ttaacagcgt atctgtttat
      361 gtactggtat ctatttatag ttgataactc tcgagatcct aaaaacttaa aagattctgt
      421 attaatctgg ttgagatatt ttaaagcatc tagtaaattt cgcagactct tttttcttgc
      481 gttttatttt actttgatat tatttaaaac cttgcttaat cgaaatatgt ggatgaaccc
      541 gctttcaaac gttattggac agtggtcatt atatattata gataatgcta caggagagag
      601 cagattgaca acagaatgcg tggagaacac gatgctgttt atcccttttt gcatgttatt
      661 gttatggtgg cgtgacatta aatgtgatat tatacgcacc atctatgttg ggattaagta
      721 tgtgttttta ttctcattaa gtatagaatt tacacaactg ttttttaggc ttggtacatt
      781 tcaactatcg gatttgtttt ataacaccct agggggctta attggtgctt tgctatattg
      841 gctattctac cgattaaata agtatataga cttaaaatag aactgctttg aattttaata
      901 tggttaatat ttttctgcag acaggattgt gtaatctcat caagaatact ttacatgcca
      961 tctatttttg ctaaaacatt gttgttatat cttcaagagg tagggaattt tattgcttta
     1021 aatgaggtcg agcgaattaa aaaattggta aaatatataa catggtggta gttatggtaa
     1081 aagtgtttga tataattaag ttttcggatt tcagaggata tactgctgtt gcattggata
     1141 atgagactct tttctcgatt ggtattgagt ttcacattaa gcaaataaat caaggattta
     1201 gtcataataa atatactatg cgagggctac attatcagag tggagagcat gctcaaggaa
     1261 agatagtgat gtgcctacat ggtgaaatta tgaatatcgc acttgatatt cgacaaggat
     1321 cttccactta tggatgtgtg tatgtcaaca cattgagtga aagcaatggt aaatgtgcgt
     1381 atatcccacg tggatatgct catggctatc ttactatgga ggacaatacg ctagttcagt
     1441 ggtgcgtaga taatgatttc aattctaatg ctgccaaatg tataagttat gaaagcgtag
     1501 atattgaatg gccggtcagt cggcagtgtt tcgttataag tgagaaagac aaaaatgtgg
     1561 aaagattaag cttttgatta aaatgagctg tggagaaaag aaggatactg gaaaataaga
     1621 agaacaataa ccgagcaaat gctcggttat ttctatgagg aatacataaa actaaattta
     1681 gatatagat
//
LOCUS       DAZKCN010000182         1665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000182 DAZKCN010000000
VERSION     DAZKCN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.193"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..506
                     /locus_tag="ACG8K3_002678"
     CDS             132..506
                     /locus_tag="ACG8K3_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP9146477.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="ACG8K3_002679"
     CDS             complement(620..1249)
                     /locus_tag="ACG8K3_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9146478.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="ACG8K3_002680"
     tRNA            complement(1383..1455)
                     /locus_tag="ACG8K3_002680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          558 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tactt
//
LOCUS       DAZKCN010000183         1612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000183 DAZKCN010000000
VERSION     DAZKCN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.194"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..1540)
                     /locus_tag="ACG8K3_002681"
     CDS             complement(77..1540)
                     /locus_tag="ACG8K3_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFP9146479.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
BASE COUNT          462 a          332 c          291 g          527 t
ORIGIN      
        1 ataattcatc aatagcttgg ttatcaaact cttttccacg atcagtatga aaaatctcaa
       61 cctctgtcag aggttgtcag actgtagaca aaagctgttc caaactcgta actcgagctt
      121 ggaacagctt tttatctact tcgtttcgaa tttttgaaca aaaaaataaa ggctccttgc
      181 ctttccatag ccaggtggct aatttcttta aattcattgc agcaaacact agcatcgtat
      241 gcgtcgcgac tttttccaag cctcgatact tggtccaacg catcccatgc ttttcttttg
      301 catccgcaaa tattcgttca atcgtttgtt tccgtttttt gtaaattgat ttattcagat
      361 ctgttaggcg aagatgttcc acttcatcca tcagatctcc ccaaacatgt ctcgtgatga
      421 ctcgttgatg atttttactt ttggtacatt ggtttagcag aggacagttc acacattctt
      481 ttggattgga tttgtattct cggtaccctt ctcgagtaac cgttgaaaaa gcgagcattt
      541 gatcttgcgg acaaatataa caatcaaaat actcgtcata gagaaactca ttttttgata
      601 atagcccctt tttccctttg ggccttgaat aaggcaaaca aggtcgaagg ttcaaatgag
      661 tcaaaaagtg aacaaactta ggtgtcttat acccagcatc agcgacaaca caattgattt
      721 caggaaaaag tctcttcgat tgtttgacga gctgaaaggc gacttgactg tcatgaatat
      781 ttccaggagt gatgattgtg ctaagaataa atccattgtc gtcgcatgaa gtatgggcag
      841 aataagcaaa ttgtttttct cgttcgcctt tgacgtagta gccgctttcc ggatcagctt
      901 tacttatttt tctttctttc agcttcactt ccttttctaa gtccaaagta aaagggcgtt
      961 ttccagcctc tattcgttga cgattgatct catcttctaa ttcatcctga taggctttcg
     1021 cttcatcatg ggtcatttct ttcgtaaact tatgtttatt ggcgttagct ttgatgtggg
     1081 tagaatccag atacaaatta tcctctgtta caaaacctgc tttaactgct tgttctaaaa
     1141 tataggcaaa aatatcctca aacaccgtcg tttctctaaa tcggcgaaca taattctttc
     1201 caaaagttga gaaatggggg attttctcct cgaaggagta gcctaaaaac cagcggtaag
     1261 cgacattggt atcgacttcc ttaatggttt gtcgcatgga acggataccg aataaatact
     1321 gaataaacac caatttgatc agaatcactg gatcgatact tgggcgacct aaatcggagt
     1381 aagtggcttc gacgattgga taaataaatt caaaatcaag cgcggcatca atctttcgaa
     1441 cgagatgatc ttgaggaact agatcatcaa tcgtaagcat cgcaaattga tttctaccat
     1501 tcgtattgtt tttagacatc atagctatca ctccttgcat tcgttttcat aaatctatta
     1561 taaaagaaaa gactgtagac atcatccata aaatggactt tgtctacagt ct
//
LOCUS       DAZKCN010000184         1593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000184 DAZKCN010000000
VERSION     DAZKCN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.195"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8K3_002682"
     CDS             complement(52..1518)
                     /locus_tag="ACG8K3_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP9146480.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          528 a          333 c          291 g          441 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tat
//
LOCUS       DAZKCN010000185         1583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000185 DAZKCN010000000
VERSION     DAZKCN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.196"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..530
                     /locus_tag="ACG8K3_002683"
     CDS             12..530
                     /locus_tag="ACG8K3_002683"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9146481.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            658..1341
                     /locus_tag="ACG8K3_002684"
     CDS             658..1341
                     /locus_tag="ACG8K3_002684"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP9146482.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT          532 a          252 c          325 g          474 t
ORIGIN      
        1 agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta
       61 tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt
      121 atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac
      181 gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag
      241 gaaaaaaaat ctcgacccgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata
      301 caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg
      361 gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat
      421 ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata
      481 cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa
      541 aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact
      601 catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg
      661 cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc
      721 cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa
      781 gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag
      841 cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg
      901 acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa
      961 gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa
     1021 gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca
     1081 aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt
     1141 ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc
     1201 cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat
     1261 cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa
     1321 tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg
     1381 agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca
     1441 ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa
     1501 ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa
     1561 aataattcat tatttgctaa tta
//
LOCUS       DAZKCN010000186         1532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000186 DAZKCN010000000
VERSION     DAZKCN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.197"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /locus_tag="ACG8K3_002685"
     rRNA            <1..1342
                     /locus_tag="ACG8K3_002685"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1532
                     /gene="rrf"
                     /locus_tag="ACG8K3_002686"
     rRNA            1437..>1532
                     /gene="rrf"
                     /locus_tag="ACG8K3_002686"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          450 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tg
//
LOCUS       DAZKCN010000187         1462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000187 DAZKCN010000000
VERSION     DAZKCN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.198"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8K3_002687"
     CDS             <1..1412
                     /locus_tag="ACG8K3_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9146483.1"
                     /translation="FGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRV
                     VGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTH
                     YQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENY
                     WKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKT
                     DTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRL
                     VYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQE
                     LRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIK
                     ELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAE
                     TLDQLLPWNKDVQQHFSRN"
BASE COUNT          483 a          263 c          312 g          404 t
ORIGIN      
        1 tatttggacg ctctaaagag actctctttg aggaaacgaa tgggcaattg aatcttttta
       61 gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt ccagtaaaag
      121 gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct ataacagaga
      181 aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca cagatgaaag
      241 atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg ttggattgtc
      301 tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga ctttcttctt
      361 ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc aatagtttag
      421 tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg caagagaact
      481 attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat tggcatatta
      541 aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat cttaatcaag
      601 aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc aaaacagata
      661 cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt tattatcacc
      721 acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt acagggtatt
      781 tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt gtctattgtt
      841 tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa aacacggata
      901 ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa aagaaatgga
      961 aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt ccattattta
     1021 ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag ttggatgcag
     1081 ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa gatggtcgtt
     1141 tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata ggacggaaaa
     1201 attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt ctgagtgtat
     1261 ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt ctatttgata
     1321 agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg ccttggaaca
     1381 aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg attctattct
     1441 ataggtcggg ttatttgacg ta
//
LOCUS       DAZKCN010000188         1432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000188 DAZKCN010000000
VERSION     DAZKCN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.199"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..457
                     /locus_tag="ACG8K3_002688"
     CDS             47..457
                     /locus_tag="ACG8K3_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9146484.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            471..827
                     /locus_tag="ACG8K3_002689"
     CDS             471..827
                     /locus_tag="ACG8K3_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP9146485.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          529 a          210 c          268 g          425 t
ORIGIN      
        1 aagaaattac tgtagaattg gattataatt aaaaaggagt gagaaaatga taaaagtata
       61 tacttcgcct agctgtactt cctgtagaaa agcaagagca tggttgcaag cgaatcaact
      121 tgaatttgaa gagaagaata tttttgccga accgttaaca gaaagtgaga tcaaaaagat
      181 cttggcagcg actgaaggag gagttcaaga tattatttcc actcggtcga aaatttacga
      241 aaagctgggc atagatttta acgaattaac tttaaagcaa atgatcgtct tattcaaaga
      301 atatccttca ttattgagac gacctatttt gattgatgaa aaaagaatac taatcggatt
      361 taatgaagat gagatccggt catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct
      421 gatctattta caaaacaata acatcttaaa ttcataggga gggagccttc gtggaagtga
      481 aagtctatgg atcagcgcac tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc
      541 atcaattgcc ttatcaattt gttgacttag aagaacgcga tctaagtaca gaagaagcag
      601 aagaattatg cagttttgaa gaagtgcaag cagaacagct atttgcgact tggtctgagg
      661 cttttcacaa cattgcgatt gacctttcct gtgtaggaaa aaaacaactg atttttttat
      721 gcacacaaca taccaatctc ctcagacgtc ctttgattat tatcgatgaa gttctattta
      781 ttggatacaa tgagcagttg atggaacagc ttatcttaaa taaatagact ttaactgttt
      841 taatcaagtc actgagcatt tcatacagtt gtaacagtcg aacacttagc atcaagcgtg
      901 ttatgtgttc tttttttgac tttatttttc tactatagag acatgctcaa tttatgtggt
      961 caaaattcaa atctaggtgg ggatatagag ttaaaaatat attgacatgt gtaaatttac
     1021 atgatatatt catttctgtt gttttaagta gcaaagcaat aaaatagtat tttaaaaaac
     1081 atataaaata gttgttgaca tgttagaaat gacatgataa gataactgag ttgttaaaaa
     1141 agaaccactt gtgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
     1201 acaaatgata agttatgata aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa
     1261 acttttaaaa aagagttgac aattacttgt ctctctgata tgatataaaa gttgctacgg
     1321 cagccaaaaa cgaatcgggg aacactcctg atgaagtaga cctttgaaaa ctgaacaaag
     1381 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa ca
//
LOCUS       DAZKCN010000189         1424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000189 DAZKCN010000000
VERSION     DAZKCN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.200"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8K3_002690"
     CDS             complement(150..1337)
                     /locus_tag="ACG8K3_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP9146486.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZKCN010000190         1418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000190 DAZKCN010000000
VERSION     DAZKCN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.201"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..426
                     /locus_tag="ACG8K3_002691"
     CDS             <1..426
                     /locus_tag="ACG8K3_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9146487.1"
                     /translation="SGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIF
                     SLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGK
                     ISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            656..886
                     /locus_tag="ACG8K3_002692"
     CDS             656..886
                     /locus_tag="ACG8K3_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP9146488.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1109..1207)
                     /locus_tag="ACG8K3_002693"
                     /pseudo
     CDS             complement(1109..1207)
                     /locus_tag="ACG8K3_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          458 a          233 c          226 g          501 t
ORIGIN      
        1 agtggaaata ctagtcttga tttagaaaat atgtttcttc gcgtaggagt tcaagcaaaa
       61 gctgtactag accctcattt tcaagcccaa gtcgcttctt tattaacagt taatgattta
      121 gtaatcattt tttctttatc tgggaaaacg aaagatactt atgattcatt gaaaatagct
      181 aaaaaaaatg gcgcaaaaat tattgctata actaactata ttcattcacc tataggaaag
      241 tctgcagact tagttttaca aacagccatt gaagaatttc ttaatggtgg atctttagct
      301 ggaaaaatat cacaacttta tatttgtgat ctattagttc atggttatga acaaaacaat
      361 aatattgatt cagttgaatt aagagaaaaa gttttgcgct ctattattga taaaagtatt
      421 gagtgaacta cccccactta gctaaaccta acggttttta acgattgaag taggcaattc
      481 ttgggatgag agcatacttg atagcttatt ttttactaat agcggtggct cttattgacc
      541 aaatatccct gtagttccta cggttcttga ttttatctaa agatattttt atcgtggaag
      601 ctattgatca acttgaaaga aaaacgtgta gctcattatc actagtttat cgagtatgga
      661 tgaagcaatg gatcatcctc tcttaaataa atttattaag gatctgatcc ttcaaatttt
      721 ttccatgatc gatgagcagg aacctactga atctaaacga catcgggcac aaggaattat
      781 taagattgct aagcgaatgt tttctataaa gggtgttcta agctatacag tgttaattca
      841 aaagattccc aatcatcttg tttataagaa caacgttgaa gattaaaaaa gtgtttcaat
      901 gctaaaattg caaaatactt caatgttatg agacaaactg tctataagaa tcaatttcca
      961 cccatttcat cacttgttaa aagtttatga tccttttgat ttttatatta atttatctgt
     1021 atttaataca gattttattt attagttcat ctgcctattg tattttattt tgttgcctta
     1081 ccatctgaag taaatatact cttaattgtc aaattaagtt agacagatca tcaatactta
     1141 cgtaactcaa aaatacttcc aaaggtgttc gataatccag tgattttcta ggaagattat
     1201 ttctgcgcca gcattttcta atgtttgaat ttgtgttttg taatcttggt tgcttgtgct
     1261 aattcttgaa tagccgtaaa ttgccaaaat ttcatcaccc tttctggaca taagtttctg
     1321 acaccagtta aaatcaagtc tatcagtaac ccatacaccc gtgaggacgg gtgtattttt
     1381 aatcccccta gaaggggtcg acactcgctg ccccttaa
//
LOCUS       DAZKCN010000191         1408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000191 DAZKCN010000000
VERSION     DAZKCN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.202"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            360..902
                     /locus_tag="ACG8K3_002694"
     CDS             360..902
                     /locus_tag="ACG8K3_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146489.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFKKNTSEEKNLKL"
     gene            1058..>1408
                     /locus_tag="ACG8K3_002695"
     CDS             1058..>1408
                     /locus_tag="ACG8K3_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP9146490.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPD"
BASE COUNT          551 a          171 c          269 g          417 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa attgatgaag cagttcccaa
      181 agggctgctt catcaatttt ttcgtaagtg tccgtacact tactgtgctt gctcatatat
      241 taatcgatca tatactaatc gtgtaagaac aactttaagc ataatactta tattgttata
      301 ccatagtttg gaaaaataaa aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa
      361 tgttattgaa agtagctata aaatgctatt gtgtaaaaaa taaaggagga ataatttatg
      421 aaaaaatagt accaagagga gcatgcctat tacttgcatt atgtttgagc ttaagcgcag
      481 ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga aggacaatcc ataagttctg
      541 aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga aacttttaag ccatcagatt
      601 atacattgga ggcaaagaaa gaatatgttt atgaatattt agggcttaaa tttaaactct
      661 cagacaaatt tagaaattat atagctgata aaaagatagc gatgttagat gatcaaagtc
      721 ctatagataa agaactaaag tatgctattt tgacatttga gaaaatgaca gaagaacaaa
      781 agaatgcagt tatagaaaag atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa
      841 gaattgggac tattggtata tttaaaaaaa atacatcaga agaaaaaaat ctaaaattat
      901 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaatacg attgctattt
      961 aagcacaaat agcggagctg aaagtaatct tctggatgaa ttaaaaagaa ctgagattca
     1021 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
     1081 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
     1141 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
     1201 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
     1261 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
     1321 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
     1381 ggcttcatat cctttcttaa tgccagat
//
LOCUS       DAZKCN010000192         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000192 DAZKCN010000000
VERSION     DAZKCN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.203"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..159
                     /locus_tag="ACG8K3_002696"
                     /pseudo
     CDS             1..159
                     /locus_tag="ACG8K3_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1135..>1352)
                     /locus_tag="ACG8K3_002697"
     CDS             complement(1135..>1352)
                     /locus_tag="ACG8K3_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146491.1"
                     /translation="PGTPSQRGLNEHSNGLLRKNGLPKEMDFNQVNQGFISSVASKRN
                     HIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          479 a          179 c          212 g          482 t
ORIGIN      
        1 cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag
       61 accattcgag gaatatataa aaagaactga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaaa tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga acccattagc aatatataaa agaaggtatc
      241 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      301 taagaactaa ataggaaaac gtagacgtaa ttttctacgt tttccttttc atatatatta
      361 aatattacgg aaaattatat aaactaattt agtgttttgt ttgtaattcc taattttgct
      421 ccacttttag ataattttta ggtctattta actcattaga cattctatgc tcatttttct
      481 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      541 gtacttttat tacgatacta gtatgatata tatctacagc aaacagttaa aatcttccta
      601 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      661 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      721 cttatactta ttgcgcaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      781 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
      841 ttagtataaa ttaacatact actatatttt aggtttcata ttatataaat taataatata
      901 ctaccatttg ttggtagtat aatttaaagg taataagtta ttcttttatt actatgtttg
      961 ggttttatag caaagaaagt ttaataagag tttggaccta gcaaacaata ttaatgaggt
     1021 cattaaatct tattatcttt agtgatataa tgaaatatct acacgacaaa attatgatct
     1081 tgcgtaaagt tataaaatat taataaaaat attttattaa tattatttgt caaattaagc
     1141 gagacaaaac tttccattta cgtaactcaa aaaaacttct aatggtgttt ggtaatttag
     1201 tgattttcta gggatatggt ttcttttgga cgcaacggat gagatgaatc cttgattgac
     1261 ttggttgaag tccatttctt ttggtagtcc attttttcgg aggagtccgt ttgaatgttc
     1321 atttaatccc cgttgggaag gcgttcctgg gt
//
LOCUS       DAZKCN010000193         1267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000193 DAZKCN010000000
VERSION     DAZKCN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.204"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..>1267
                     /locus_tag="ACG8K3_002698"
     CDS             17..>1267
                     /locus_tag="ACG8K3_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP9146492.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENM"
BASE COUNT          423 a          249 c          248 g          347 t
ORIGIN      
        1 gaggacgata cgctatatga atgattctat caaaaaaatg ctgagaataa cagagaaaga
       61 tttgatgatt acagaggtct cttacgagac ccttcagaag aaaaagacgt tggtcgtcga
      121 tgctgttctc tcgcctactc ctcgtgcttg tagaagttgt ggttctactg tggtagatgg
      181 aaacgggaaa gcaattatag tgaaaaatgg aaaaaaggaa acgattgtcc gttttgaaca
      241 atacaatcat atgcctttgg ttatgcgcct aaaaaagcaa cgctatacct gtaaaaactg
      301 tcgcacccat tggacggctc aaagttattt tgtccaaccc agacattcaa tcgcaaatca
      361 tgttagatat aaaattgctt ctttactgac tgaaaaagta tctttatctt ttattgcgaa
      421 aagctgtcag gtatctttga ccaccgttat tcgtacattg aaagagttta aaagctattt
      481 accaaagcaa tctaagaaga tcctccccag agtattgatg gttgacgaat ttcgttcgca
      541 tgcttccata gaagataaga tgagctttat ttgcgcggat ggcgaaacag gaaaattaat
      601 agatgttttg cctacgcgta aattacctcg attaacaagc tatttcttag gttgtaccaa
      661 tccagaagaa gtagaattct tggtgacaga catgaacgcc gcctacttcc agctcaccaa
      721 acgtgttctg ccaaatgcga aagtcgtgat tgatcggttt catattgtca aacacatgaa
      781 tcaagcgttc aatgagttgc gtatccgtga aatgaatgaa cttcgtaaag ccggacagaa
      841 aagccaggca gaaaaactga aaaagaactg gcgctttttg ctaaaaaatc gtgcaaacat
      901 caaccattat gaatacaaaa catggaaaag tttccgagca ccaaaatacc catttcttac
      961 tgaagcaatg atgattgatc gattgcttga gttttctccg cccctaaagg aggcgtatcc
     1021 cttttttcat gaattagttg aagcctttcg agacaaagac cctgacttat ttttctcctt
     1081 attggcagaa cttcccgaaa cgttagatga cagctttcgg gaaaagcttc aaaaccttct
     1141 gacctatgaa gaaggcatca ccaacgcaat gatctatcct tattccaatg gaaaaataga
     1201 agcgaagaat acccacataa agacaatgaa acgagtatcc tacggattta aatcatttga
     1261 gaacatg
//
LOCUS       DAZKCN010000194         1258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000194 DAZKCN010000000
VERSION     DAZKCN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.205"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..1160
                     /locus_tag="ACG8K3_002699"
     CDS             30..1160
                     /locus_tag="ACG8K3_002699"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9146493.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKE
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          458 a          207 c          245 g          348 t
ORIGIN      
        1 tacaaaaaac aaagaaaaga gtgagtgaaa tgagcaaaaa tagaccattt acagtagatc
       61 atgtcggaag tttcttacgc ccgcaatcga tcaaagaagc aagaaaaaaa gttcaagaag
      121 gcttactttc aaaagaagaa cttcgtgcaa tcgaagacca ggaaataaaa aaattagtgg
      181 aagaccaaaa agcagtcggc atcaaaggaa ttacagatgg tgaatttcgg cgtggatttt
      241 ggcatcttga ttttttagaa gaactaaatg gtatcgaagg ctatgttcca gaaactggat
      301 ataatcaaac attccacgga aaagcagcac cagcttataa tattcgggta gtagataaat
      361 tatctttcaa tccaaatcac ccatttttaa aagattttgc ttttctgaaa gaagtagtgg
      421 aaacagatga tacttttgtt gcaaaagcaa cgatccctag tcccacgatg attttacgac
      481 aagagatcct atctaatgat ggttcttcaa aaatcaagga gatttatcct gatctatctg
      541 atttttatga ggacttgtcc tctacttaca aagcagcgat tcatgctttt tatgaaaaag
      601 gttgccgtta cctgcaattc gatgatacaa attgggcgtt tctagctgat caaactaaaa
      661 gagaagcact tcttcaaaaa gaaattgatc cgaaagaaat ggcccatatt tgtacagaga
      721 ttatcaataa cgcactggaa gacaaaccag aagatttgac gatcacaaca catatctgcc
      781 gcgggaatca cgcttcttct tggctgtttt ccggtggata tgaacccatt gcagaagagt
      841 tgttttcaac aaattatgat ggtttctttt tggaatacga ttctgaccga gcaggagatt
      901 ttactccttt aagacattgg agtaacaaag aaagtaaagt tgttttagga ttagttactt
      961 ctaagtttcc agatttggaa gatacggaga aaataaaaga acgaataaaa gaagcacaac
     1021 aatttgtacc attggaaaat ctaagtatca gtcctcaatg tgggtttgct tcaacagaag
     1081 aaggaaacca tttaacagaa gaagaacaat ggaagaaaat ccaattattg cagcaagtcg
     1141 caaaagaagt ttggctataa aaatgtgagg cttggcaaaa aaaagcgtcc caaaaagtcg
     1201 gcttttcatg gagtggagaa atccactctt ttttatttac cttttatatt atttattt
//
LOCUS       DAZKCN010000195         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000195 DAZKCN010000000
VERSION     DAZKCN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.206"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8K3_002700"
     CDS             <1..1076
                     /locus_tag="ACG8K3_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9146494.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZKCN010000196         1172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000196 DAZKCN010000000
VERSION     DAZKCN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.207"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..357)
                     /locus_tag="ACG8K3_002701"
     CDS             complement(<1..357)
                     /locus_tag="ACG8K3_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP9146495.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMF"
     gene            complement(510..836)
                     /locus_tag="ACG8K3_002702"
     CDS             complement(510..836)
                     /locus_tag="ACG8K3_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146496.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(848..1048)
                     /locus_tag="ACG8K3_002703"
     CDS             complement(848..1048)
                     /locus_tag="ACG8K3_002703"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146497.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
BASE COUNT          345 a          199 c          123 g          505 t
ORIGIN      
        1 gaacatttgc attttacttt tattgaagcc attttttctt ttcacaagaa gtttaacttt
       61 ttgaagttca tctagtgctg ataagattgt attttttgac attcccgaaa tttctacgag
      121 atttttgatt tcaaatatca aaaaaatttt ttgattctca tcatacaagt tatttttcaa
      181 tgaaagtcta tttctatctt ttagtaacat ataaactaac ttagaactta gtttcatttt
      241 cttatatttt tcatttacgt ttaaaaaaat aggatattga tagtataatt ctgcatatat
      301 atcagaatta gaatagcctt gcttttcaac taagctttca acttcttttt cattcattgt
      361 cacacctgct tctgatttta tttgctataa tagattcaaa taaagttttc tcagaacttt
      421 attattgagt gttcccccga tctgcaaact gacgggaaca ctctttttaa ttttttctac
      481 ttcatcaaat ttaaaaaacg aggtgttttt taaatttgat gactcaatat ccattttttg
      541 cattcttctt ttgaaaacaa ctccattttt tctaataaaa taactggcaa tcccattctt
      601 ttaaatttca aaaaagttgt atatgataca ttcaaatatt tagccatctc ttttcctttt
      661 agaaatggtc tttctaatcc ggtatcaatt cttgcttgat caatagcctc ttttgtcatt
      721 tcaaaaacat aatttttcaa ttgattcgtt tgttgtttat ctaacattac tgtaattcct
      781 gcatccatta tttttctcct ctcattttct atttcttcaa tcagccaact cgccaagttg
      841 tatttattta gattttcaaa tcattaagta accaatttac caacttttga taagtagatg
      901 ctctaatact ttctttgctc ccattttcga ttttgacata tgttttccga tttataccta
      961 tcaattttgc tgcatcgttt ttagtaagta taagatcggc tctatgtctt cttaagcttc
     1021 taatgaattg atcattaaga gttatcattc agcgcctcct ttcaaattat tcactttgtg
     1081 gtttttatct taataagcac attttaaacc acaaaacgag taatatcaat taaaatatta
     1141 ctcaatttgt ggttttcatg ctataatagt tt
//
LOCUS       DAZKCN010000197         1126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000197 DAZKCN010000000
VERSION     DAZKCN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.208"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..760)
                     /locus_tag="ACG8K3_002704"
     CDS             complement(206..760)
                     /locus_tag="ACG8K3_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9146498.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          363 a          217 c          130 g          416 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatacttcc gaaaattcag ttatcttcat taatctcacc ttctaagttt tcattataca
      181 caaaaaatat atcctaatat ctcaattagc ctcccaattt aaaaccacgt aacgatgccc
      241 ctatttcatc ttcagttata ccaatgtacc tttttgtaat tgcttgcgag ctatgattaa
      301 aaataaacat taaggtagca atgtctctag ttttcttgta gtagtgatac ccaaaagttt
      361 ttcttaatgt atgtgttcct atatcatctc ttcctaattt ttctgctata tctgcatata
      421 tctgatagac acgatccctt gttatatgtc gaccttgttg ccgacttgga aataaccaat
      481 cattttcttc tttaccttca gtatataata aaatctcttc tctcaaatta tctataaaaa
      541 tcggttttcg cttacctgtt ttatgttcaa caataatagg cttactttta cctcgtatgt
      601 ctccaacttt taatcctata atatctgaca tacgtaaccc attattgatt ccaaacaaaa
      661 ataaaaaact atctctttct ggattacttg ttcttcttag ccaaaactga aaatcttgta
      721 tctcttcatt tgtccttaac ggttgtacat tatagctcac tattgtttcc cttcttaaaa
      781 atattctgag ttcatgatta tatatatatt ttaccataat catgaactta tatatatact
      841 ctgttaaaag tgaagaaaat caaggggttt agtttatgat tttatatcat ttcagtaact
      901 cagaatattc cgactttttt gtacaaaaaa attaacctta cttatatgaa gattctaagc
      961 tcttatttaa attttagacg ggcagttatc cgtagaagtt cttagaatag ctaatttaca
     1021 taattaaatt atatgaaatt taagtttgaa caatatacta agaactcccc ttactataaa
     1081 gtgaaataaa ttagcatatg aataaccgtg ctaccaaact tttcat
//
LOCUS       DAZKCN010000198         1102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000198 DAZKCN010000000
VERSION     DAZKCN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.209"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..834
                     /locus_tag="ACG8K3_002705"
     CDS             301..834
                     /locus_tag="ACG8K3_002705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9146499.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          355 a          205 c          202 g          340 t
ORIGIN      
        1 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaagttgtga
       61 aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa aatggttcct cacaactttg
      121 cataaatttg acttaatacc gagaagtcat gatccccatc taactccaaa tcacaaggaa
      181 tgagttcaaa aactagcttt tctcaacaaa tattacataa aaaattctat gttttttgtt
      241 aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg ttttacagaa
      301 atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag tgcttgttct
      361 cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac attgatacag
      421 cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca agaagacgta
      481 cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt tcaaaaaaat
      541 cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg atgcttagat
      601 tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag tagccgtcgc
      661 ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt gaacatccaa
      721 ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat cctgctttat
      781 caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa gtaaaagaat
      841 acttatgaaa cacacaaaga ggtcgtgagc ctaccgtttc actccgagtc acaaggagca
      901 atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg aggagctggc
      961 aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     1021 cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta ccgcagatgc
     1081 aagcttttgg acaccgttta cc
//
LOCUS       DAZKCN010000199         1070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000199 DAZKCN010000000
VERSION     DAZKCN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.210"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1070
                     /locus_tag="ACG8K3_002706"
     CDS             <1..>1070
                     /locus_tag="ACG8K3_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9146500.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFY
                     SSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTS
                     INGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKP
                     QQREMLLDALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DAZKCN010000200         1070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000200 DAZKCN010000000
VERSION     DAZKCN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.211"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(676..>1070)
                     /locus_tag="ACG8K3_002707"
     CDS             complement(676..>1070)
                     /locus_tag="ACG8K3_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFP9146501.1"
                     /translation="VFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKI
                     PREYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQAL
                     KYARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          360 a          143 c          194 g          373 t
ORIGIN      
        1 tgacggtatg tggttaaaaa agaaaaatag ctaggagatt tttgagagat gactggtaaa
       61 tttaaaaatg atagaaatgg agagtatgta tgagtaataa acaattttta tggtcttctt
      121 ttgcatttat tgttgttgat atttttttcc gatattttaa taacgatatg atatttaact
      181 ggcaaagaat tataatcgat tactgtattt tttgtataat atatttttta gtgaaaacat
      241 ttctaaaata cgcaaaaaaa taaaattgaa gatgttatta aatcgcgatt atttacggag
      301 atttatataa ataaaaaccc cctgacgaag tcgaaggggg tttttatttt ggtttgaggt
      361 ttgcatacct accgaaaagg tagggcagca aatcgtcaaa caggtatcag ctaattgcac
      421 cataagggtg cgctgatact cggctcaaaa gagccgaaag agccgtctgc aagacccctc
      481 ggcgagccca cactttgctt gcaaagtata gtgggttata ctttacatgg aagtaattgc
      541 cggaaatagt gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag
      601 ttatgcagtt tacgggccaa gattgtttaa gcaaaataag ataagttaag ggatgcagtt
      661 tatgcacccc ttaacttatc tattaaataa tttatagcta ttgaaaagag acaagaattg
      721 ttcaaagcta atattgttta aatcgtcaat tcttgcatat tttaaggctt gataaaattg
      781 gtttttggaa aatagtttta tgtattcttt gttaacccat ttcataacaa aattttcata
      841 ttgttttcta tctttgagtg atatttttga agggtgtctt tttaatacaa ttaacgagct
      901 attaactcta ggttttggat gaaagtattc tctagggatt ttacttaata tggatatatc
      961 aacttctgtc attaaaaata gtgctagtga acgatttgta tttagcaacc ttttagcaaa
     1021 tccatattcc actattaaat aactttctgt agctgtgctt tcaaaaacaa
//
LOCUS       DAZKCN010000201         1036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000201 DAZKCN010000000
VERSION     DAZKCN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.212"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..906
                     /gene="scrK"
                     /locus_tag="ACG8K3_002708"
     CDS             34..906
                     /gene="scrK"
                     /locus_tag="ACG8K3_002708"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP9146502.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          302 a          204 c          254 g          276 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcaggaat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaaa
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataa
//
LOCUS       DAZKCN010000202         1025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000202 DAZKCN010000000
VERSION     DAZKCN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.213"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8K3_002709"
     CDS             complement(53..787)
                     /locus_tag="ACG8K3_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP9146503.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          360 a          211 c          183 g          271 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctca
//
LOCUS       DAZKCN010000203         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000203 DAZKCN010000000
VERSION     DAZKCN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.214"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8K3_002710"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8K3_002710"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9146504.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          262 a          221 c          157 g          361 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt t
//
LOCUS       DAZKCN010000204          966 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000204 DAZKCN010000000
VERSION     DAZKCN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.215"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>966
                     /locus_tag="ACG8K3_002711"
     CDS             <1..>966
                     /locus_tag="ACG8K3_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP9146505.1"
                     /translation="EIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRT
                     GFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLF
                     EKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATY
                     IPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSK
                     EEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIY"
BASE COUNT          347 a          182 c          166 g          271 t
ORIGIN      
        1 gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta
       61 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
      121 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      181 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      241 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      301 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      361 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      421 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac
      481 attccactaa aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga
      541 gaagacggca ctaaagaagt actgagttat gcgattgctc caactgaatc aacatacgtt
      601 tggaatgagc tgctacagga tattaactcc agaggagttc aagaagtctt gctttttatt
      661 acggacggct taaaaggcat gaaagatact atccatcaaa tttatcctaa agcaaaatat
      721 cagcattgtt gtatccatgt atctcgtaac atcgctcata aagtacgtgt caaagaccga
      781 aaagaaatct gtgatgactt taaggctgtt tatcaagcta actcaaaaga agaagcgaat
      841 accttcttat ccggcatgat tgagaaatgg aagaaaaact atcctaaagt gacgcagtca
      901 ctcatagaaa accaagactt attaactttt tatgattttc cacctagcat tcgtagaacc
      961 atttac
//
LOCUS       DAZKCN010000205          955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000205 DAZKCN010000000
VERSION     DAZKCN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.216"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..836
                     /locus_tag="ACG8K3_002712"
     CDS             96..836
                     /locus_tag="ACG8K3_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146506.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          345 a          173 c          191 g          246 t
ORIGIN      
        1 cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct gtgctattat
       61 tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga cgaaaacatt
      121 tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac aagaagcgag
      181 tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg tttgaacaga
      241 tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag cgtcgtgcaa
      301 caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa ggaaaattac
      361 cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa aaactttttg
      421 gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag catactacac
      481 gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct aaatatgtac
      541 ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat ttaatcgaat
      601 cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg ccgtatcaag
      661 aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg ttagaggaaa
      721 caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa cgctcattac
      781 aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt aaataagtga
      841 tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt ccttatttta
      901 tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag cgaat
//
LOCUS       DAZKCN010000206          954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000206 DAZKCN010000000
VERSION     DAZKCN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.217"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..>954
                     /locus_tag="ACG8K3_002713"
     CDS             261..>954
                     /locus_tag="ACG8K3_002713"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9146507.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMK"
BASE COUNT          341 a          170 c          172 g          271 t
ORIGIN      
        1 atctttcgaa aattccacag agattgcatt aaaataaatc aatcgacttc taccataatg
       61 attaatttgt tcatcaaact tattgacgtg tttcacgatt tttagtgaaa aaaactgaaa
      121 tataaaaacc ttattctgtc aaggaaatag aaatatagta agaaaaacct ttatttatca
      181 gcttttttag acaatttttt ctgttacaat agtcaaggat ttgtgaaaaa atcaatgaaa
      241 accgaaaggc gtgtaaaatt ttgattaaat cagttgtgtt tgatgttgat gatacgatgt
      301 atgatcagca gcagccattt cgaaacgcgg tcaaaagagt cgttccgctt gtttcagatg
      361 cagatatgca tccactatat atccggttcc gccatcatag tgatgaaaac ttcccaaaag
      421 taatggcagg agattggaca ctagaatata tgcgagcaca tcggatcagc caatcactaa
      481 aagatttaga ctaccctcat attacggaag aagatggcct cttatttcaa aaaatttacg
      541 aggaagaact tgataatatc tgcttgcatg aggaggtcaa aaaaaccctc gatttcttga
      601 aggaaaagaa tgtcccatta ggaattatca caaatgggcc gactgaccat cagacaaaaa
      661 aactgaagca acttcaattg aacaattgga ttccttcccg caatatgctc atttctcaag
      721 caacaggttt ccaaaaacca gaaaaagaga tttttcagct agctgagaaa gaatttcata
      781 tgcttccaga agagacctta tacgttggcg ataattacga caatgatgtt ctaggtgcaa
      841 aaagtgctaa ctggcaagcg ctttggttca accatcgaga acgcaaaatt gaacagactc
      901 caatttgcga tatcgagatt tcttcttttg atcagctgtt agagacaatg aaga
//
LOCUS       DAZKCN010000207          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000207 DAZKCN010000000
VERSION     DAZKCN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.218"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8K3_002714"
     rRNA            <1..>943
                     /locus_tag="ACG8K3_002714"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKCN010000208          937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000208 DAZKCN010000000
VERSION     DAZKCN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.219"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>937)
                     /locus_tag="ACG8K3_002715"
     rRNA            complement(<1..>937)
                     /locus_tag="ACG8K3_002715"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          198 a          291 c          210 g          238 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctc
//
LOCUS       DAZKCN010000209          934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000209 DAZKCN010000000
VERSION     DAZKCN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.220"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..484
                     /locus_tag="ACG8K3_002716"
     CDS             77..484
                     /locus_tag="ACG8K3_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146508.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            498..899
                     /locus_tag="ACG8K3_002717"
     CDS             498..899
                     /locus_tag="ACG8K3_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146509.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          350 a          119 c          163 g          302 t
ORIGIN      
        1 actattgtta aactaaattg actagcaaaa gtgaaacata aatttattaa ataaatgtaa
       61 aaattaggag aaatcaatgt cttatagagt acaattaata atttcagaag atgtagaaag
      121 tcagcaattt ggcactaatg ttattaataa agtaattaat ccagctcttt ctattaatgc
      181 gcctttaatt cctactgctt tatcattttc tgtaacagct gtggtgtctg agatagagga
      241 tacagaaaag ataaaaatag tagaaattga agttttaaac aagaatgaaa aacaaatttt
      301 ttctacgggt gaagtatcgg taagcttgcc tcctcaagtt aacgatataa actttaatat
      361 aaacgccaga aatgtcttgg ttgaagaagc aggagagcat tatgctgttg ttaaatttaa
      421 tggaactgag attggaaggc aaatctttga tatcaaggtc aacaaaccag tggaaaaaaa
      481 ttaaggagac gatacggatg cttgatatag taccatcaaa tacacatcgg agtggtagct
      541 taataaattt tgctgcgatt tttgcttgcg catcggtaat tctagcatct ccagttgttg
      601 tggatgcaag ttctactccg aaaactaaaa atgataatca acttgtaatt actacaaaaa
      661 atgagattaa cacaatttca agtaatgatg gcaaaatttt tgatgtgata aatacggttt
      721 taaaaagaaa agctgaaatc aatataaaat atgatgaaga tttaaacttg tatttctttg
      781 ttatcaaaac aacatctgaa ttgttttcct cagattacga tgttttggat actctcgata
      841 atgtattatc tgactataaa tatatgggga aatctgtagt agcaactttg ggggaataaa
      901 atgtttgact ttaatgaata tttatctatt tcca
//
LOCUS       DAZKCN010000210          912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000210 DAZKCN010000000
VERSION     DAZKCN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.221"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>912
                     /gene="bsh"
                     /locus_tag="ACG8K3_002718"
     CDS             6..>912
                     /gene="bsh"
                     /locus_tag="ACG8K3_002718"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP9146510.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKE"
BASE COUNT          315 a          123 c          165 g          309 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga ag
//
LOCUS       DAZKCN010000211          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000211 DAZKCN010000000
VERSION     DAZKCN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.222"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..471)
                     /locus_tag="ACG8K3_002719"
     CDS             complement(127..471)
                     /locus_tag="ACG8K3_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP9146511.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(464..898)
                     /locus_tag="ACG8K3_002720"
     CDS             complement(464..898)
                     /locus_tag="ACG8K3_002720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP9146512.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
BASE COUNT          258 a          169 c          155 g          328 t
ORIGIN      
        1 atcccatttc ctttaaaaac gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca
       61 cattcctagg aaatacgcaa acttttctca cttatatagt taaatctgtt gagaaaataa
      121 tcattattat atttgttcag gatactctgg cagatcagct tttagttttt ctacttgttc
      181 attgatttgt gtgatacgtg gttcggcttg gaatttaaaa tcttcgatat cttttttgat
      241 tgcttcttgg aaagcaggaa tcacagattc agctgtttct tttgtttcga tcaatgcgct
      301 tttaaaatga ttcaggctgt tatttaaatc atttgtcaga gttgttgctt cttccaattc
      361 ttcgatcagt ttctttcttg tttcattccc gctcctgggc gccaataaca gacctccgac
      421 agtgccagca gcagctccaa ataacagtcc tttgaaaaag tttttaatca tcgcaggaca
      481 cctgctttac gattgtttct tgaatagctt ttagttcttc cgatccataa ctatcttgat
      541 gattgacaaa gtgaatagaa aaatcatcct ctttgctgta gcgagggact aaatggatat
      601 gtgaatgaaa aacagattga taggctagtt cacggttgtt attcaagata ttaagacctt
      661 tcatttctgg aaaagctttt tccaatgctt gagcaatctt aggcacacgc gaaaaaacct
      721 ctcctgcaag ttcgggatca tattcaaaaa tatctgctac atgacgttta ggcacaacta
      781 atgtgtgtcc tttcgttgct tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat
      841 atattttata gcttgggatt tcttgattga tgattttgca aaaaatacag ttttccatat
      901 aaaacatcct
//
LOCUS       DAZKCN010000212          900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000212 DAZKCN010000000
VERSION     DAZKCN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.223"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>900
                     /locus_tag="ACG8K3_002721"
     CDS             28..>900
                     /locus_tag="ACG8K3_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9146513.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          312 a          155 c          169 g          264 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat
//
LOCUS       DAZKCN010000213          876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000213 DAZKCN010000000
VERSION     DAZKCN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.224"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8K3_002722"
     CDS             complement(<1..434)
                     /locus_tag="ACG8K3_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146514.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8K3_002723"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8K3_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP9146515.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DAZKCN010000214          868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000214 DAZKCN010000000
VERSION     DAZKCN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.225"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8K3_002724"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8K3_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9146516.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          249 a          184 c          133 g          302 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaa
//
LOCUS       DAZKCN010000215          860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000215 DAZKCN010000000
VERSION     DAZKCN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.226"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..119)
                     /locus_tag="ACG8K3_002725"
     tRNA            complement(47..119)
                     /locus_tag="ACG8K3_002725"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:His,seq:gtg)
     gene            complement(122..195)
                     /locus_tag="ACG8K3_002726"
     tRNA            complement(122..195)
                     /locus_tag="ACG8K3_002726"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Trp,seq:cca)
     gene            complement(202..282)
                     /locus_tag="ACG8K3_002727"
     tRNA            complement(202..282)
                     /locus_tag="ACG8K3_002727"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Tyr,seq:gta)
     gene            complement(284..359)
                     /locus_tag="ACG8K3_002728"
     tRNA            complement(284..359)
                     /locus_tag="ACG8K3_002728"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Phe,seq:gaa)
     gene            complement(395..467)
                     /locus_tag="ACG8K3_002729"
     tRNA            complement(395..467)
                     /locus_tag="ACG8K3_002729"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(432..434),aa:Asp,seq:gtc)
     gene            complement(477..549)
                     /locus_tag="ACG8K3_002730"
     tRNA            complement(477..549)
                     /locus_tag="ACG8K3_002730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(514..516),aa:Val,seq:tac)
     gene            complement(565..636)
                     /locus_tag="ACG8K3_002731"
     tRNA            complement(565..636)
                     /locus_tag="ACG8K3_002731"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Glu,seq:ttc)
     gene            complement(651..740)
                     /locus_tag="ACG8K3_002732"
     tRNA            complement(651..740)
                     /locus_tag="ACG8K3_002732"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:Ser,seq:gga)
     gene            complement(780..853)
                     /locus_tag="ACG8K3_002733"
     tRNA            complement(780..853)
                     /locus_tag="ACG8K3_002733"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Asn,seq:gtt)
BASE COUNT          240 a          238 c          194 g          188 t
ORIGIN      
        1 caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg aatgatggga
       61 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
      121 atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc tgttctacct
      181 ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac cctaaggaac
      241 ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc gcgggacaga
      301 atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc taccgcgcca
      361 tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat cgaacccgcg
      421 atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt tcattatgga
      481 ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc ccagctgagc
      541 taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt
      601 gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta cggagagtaa
      661 gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc atgctccttc
      721 ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca aaatgagaac
      781 tccggcagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct actaccaact
      841 gagctatgcc ggaataacat
//
LOCUS       DAZKCN010000216          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000216 DAZKCN010000000
VERSION     DAZKCN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.228"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8K3_002734"
     CDS             134..307
                     /locus_tag="ACG8K3_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146517.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          276 a          134 c          104 g          308 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tc
//
LOCUS       DAZKCN010000217          821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000217 DAZKCN010000000
VERSION     DAZKCN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.229"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..>821
                     /locus_tag="ACG8K3_002735"
     CDS             29..>821
                     /locus_tag="ACG8K3_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9146518.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIK"
BASE COUNT          315 a          106 c          153 g          247 t
ORIGIN      
        1 aaaagatgat ttttaggaag tgaagagagt gcataaaatt agtgttatag tacccgcata
       61 taacgtagaa gactatttag aaaactgctt agaatctatt tccaaccaga cacattcaaa
      121 tattgaaata atagtagtca atgatggttc cccggatcga agtggggaaa ttgcagaaga
      181 atataaaaag aaagatatgc gattaaaagt gatacatcaa gtgaatggtg gtttaagtgc
      241 ggctaggaat tctggtcttg attctgcaac aggagatttt gtgtgcttta ttgacagcga
      301 tgattggata gaaccagact atcttgagtt actatatttt ggcacgcaaa cttatggagc
      361 agatatctcg gtgctaaaaa taaaaaagat ttcaagttta gaacaactaa aagaacatac
      421 aattgctgag agaaaatggg aaatatttga tagagaacaa gcaatgaaaa acttatttat
      481 aaataataaa attggctatt ctgcaaataa taaattattc aatagaaatt tatttgataa
      541 tataagattc ccactgggta aattaatgga agataaggct actacttatt tgctgatcga
      601 tcattccgaa aaagttgctg tcaattcatc tgctaaatat cactattacc aaagccctaa
      661 aagtatactg agaggaagct tcaatcctag aatttttgat tcttttgaag tccatgaaga
      721 tatactaata ttcatagata agttttatcc ggaattatca attaatgtta gaactcgcta
      781 tgtctatgaa gcaataagga tgatgatgac aatgattaaa a
//
LOCUS       DAZKCN010000218          792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000218 DAZKCN010000000
VERSION     DAZKCN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.230"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..781
                     /locus_tag="ACG8K3_002736"
     CDS             <1..781
                     /locus_tag="ACG8K3_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9146519.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          244 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct tt
//
LOCUS       DAZKCN010000219          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000219 DAZKCN010000000
VERSION     DAZKCN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.231"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..660
                     /locus_tag="ACG8K3_002737"
     CDS             <1..660
                     /locus_tag="ACG8K3_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP9146520.1"
                     /translation="YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNK
                     LSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKV
                     DKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          285 a          120 c          143 g          227 t
ORIGIN      
        1 tatatttctt tgttgtgtaa agaagaaatt atcgaattac ctaaaactaa ttccacaatt
       61 ggtattgact taggtattat ggactttgcg attctttctg atggacaaaa aattgataat
      121 aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg aacaacgcaa attgtctagg
      181 cgtgctttat tggctaaaaa taaaggtacc aatctatttg aagctaaaaa ttatcaaaaa
      241 caaaaacgca aagtagctag attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat gatcaaaaat cacgatatta tctgtattga agacttaaac
      361 acaaaaggta tgttgcgaaa tcataaatta gctaaaagta tttctgatgt ttcttggtca
      421 aagtttgtga ctaaattaca atacaaggct gactggtatg gacgaaaaat catcaaagta
      481 gataaatggt ttccgtctag tcagatttgt tcagagtgcg gacataaaga tggcaagaaa
      541 tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata ctcatcatga tcgtgatatc
      601 aatgctagta tcaatattct gaccgaaggt ctaagactac attctatggg attggcttag
      661 atccaatcat caagaaccgt aggaactacg gggatagctt ggtaaataag agacaccgct
      721 gttagtaaag aaatacgcta tcaagtatgc tctattccca agaagctccc acttc
//
LOCUS       DAZKCN010000220          772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000220 DAZKCN010000000
VERSION     DAZKCN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.232"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>772
                     /locus_tag="ACG8K3_002738"
     CDS             <1..>772
                     /locus_tag="ACG8K3_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9146521.1"
                     /translation="GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFK
                     HGYKTIICNSQNDPAKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNL
                     APNVSIVSSDNFEGGKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGK
                     IFKIPNNLSTIRREMEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKII
                     GYDGTSFIEKFFPQLTTIRQPIDEIASLIVDILLKKIKG"
BASE COUNT          272 a          128 c          119 g          253 t
ORIGIN      
        1 tggttataaa ccaaataatt tagcacgtgg acttcaagga aagtcacctc agcttattgg
       61 tcttattttt cctaatatta gtaatatctt ttactctgaa ctcattgagt atctcgaaat
      121 cgagttattt aaacatggat ataaaacgat tatttgcaat agtcaaaatg atcctgctaa
      181 agagagagaa tacattgaaa tgcttgaagc caaccaagtt gatggcataa tctcatcaag
      241 ccataatctt ggaattgctg attatgagcg tgtgagagca cctattgttg cttttgatag
      301 aaatttagct cctaatgttt ccattgtttc ttcagataac tttgaagggg gaaaacttgc
      361 agctaagact ttacaaaaaa atggttgtca aaataccatt atgattacag gaaatgataa
      421 cacggattct cccacagaat tacgtgcact aggtttttct tttcaaaatc caaatggaaa
      481 aattttcaaa attcctaata atctgtctac tatccgtcgt gaaatggaaa taaagtcaac
      541 tattataaat cacaaaccag atggtatttt tgtttctgat gatttaacag ccattctcac
      601 tatgaaaatt gctcatcaac ttaaacttaa tatcccagaa gatttaaaaa ttattggata
      661 cgatggtacc tctttcattg agaaattttt cccacaattg acaacaataa gacaaccaat
      721 tgatgaaatt gcaagtctta ttgttgatat tcttctgaaa aaaattaaag gc
//
LOCUS       DAZKCN010000221          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000221 DAZKCN010000000
VERSION     DAZKCN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.234"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..>713
                     /locus_tag="ACG8K3_002739"
     CDS             290..>713
                     /locus_tag="ACG8K3_002739"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP9146522.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYE"
BASE COUNT          270 a           79 c          118 g          246 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaa
//
LOCUS       DAZKCN010000222          710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000222 DAZKCN010000000
VERSION     DAZKCN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.235"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..167
                     /locus_tag="ACG8K3_002740"
     CDS             <1..167
                     /locus_tag="ACG8K3_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP9146523.1"
                     /translation="TQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKATTCIAGMSIGG
                     FLGGAIPGKC"
     gene            169..480
                     /locus_tag="ACG8K3_002741"
     CDS             169..480
                     /locus_tag="ACG8K3_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9146524.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            584..>710
                     /locus_tag="ACG8K3_002742"
     CDS             584..>710
                     /locus_tag="ACG8K3_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFP9146525.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          284 a           73 c          133 g          220 t
ORIGIN      
        1 agacacaact tatctatggg ggtaccactc atagtggaaa atattatgga aatggagtgt
       61 attgcactaa aaataaatgt acggtcgatt gggccaaggc aactacttgt attgcaggaa
      121 tgtctatagg tggtttttta ggtggagcaa ttccagggaa gtgctaaaat gaaaaaaaat
      181 gctaagcaaa ttgttcatga attatataat gatatatcta taagtaaaga tcctaaatat
      241 tctgatattc ttgaggtttt acaaaaggta tatttaaaat tagaaaaaca aaaatatgaa
      301 ttagatccca gtcctttaat aaatagattg gtgaattatc tatattttac tgcttatact
      361 aataaaataa gattcactga atatcaagag gaattaataa gaaatttgag tgaaattgga
      421 agaactgctg gaataaatgg tttatatcga gcagattatg gagataaatc tcaattttaa
      481 tgatacttat aatattaaat atgtctcaaa attttttgat tagaaaaata ggtatattga
      541 atcagtgaca taaaagtatt tatagtttgt agggggaatc gtaatggaag aaaaaaatag
      601 actaaatgct aaacaatgtt cagatcaaga gcttaaaaaa attaaaggag gtgctggaac
      661 aaaacctcaa ggaaaaccgg catcaaattt agtggaatgt gttttttcat
//
LOCUS       DAZKCN010000223          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000223 DAZKCN010000000
VERSION     DAZKCN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.236"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..627)
                     /locus_tag="ACG8K3_002743"
     CDS             complement(52..627)
                     /locus_tag="ACG8K3_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP9146526.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          228 a          151 c          124 g          191 t
ORIGIN      
        1 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
       61 ttttcttttt gaatatatca agcgttgggg caacatagtg atgactaatg aaacaacgac
      121 gatagacaac aagcgatcaa ggtagtctgt acccgcctgt accaagatca cacttgttgt
      181 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
      241 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
      301 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
      361 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
      421 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
      481 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
      541 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
      601 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
      661 tcataatcgt ttcttcttgt gcatttaaaa gaac
//
LOCUS       DAZKCN010000224          677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000224 DAZKCN010000000
VERSION     DAZKCN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.237"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>677
                     /locus_tag="ACG8K3_002744"
     CDS             <1..>677
                     /locus_tag="ACG8K3_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9146527.1"
                     /translation="EINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKEY
                     IIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTR"
BASE COUNT          314 a           74 c           98 g          191 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactaga
//
LOCUS       DAZKCN010000225          596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000225 DAZKCN010000000
VERSION     DAZKCN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.238"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..296
                     /locus_tag="ACG8K3_002745"
     CDS             <1..296
                     /locus_tag="ACG8K3_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9146528.1"
                     /translation="VAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNT
                     SGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            485..558
                     /locus_tag="ACG8K3_002746"
     tRNA            485..558
                     /locus_tag="ACG8K3_002746"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:519..521,aa:Ile,seq:cat)
BASE COUNT          189 a          110 c          118 g          179 t
ORIGIN      
        1 atgtagctgg atggcacatt gctaactata aatacgagta tatcttcatt atggattaca
       61 atactgggaa agaattagct cgagtaagag ctgatggaat ttatagatca gatgtaaatc
      121 aagcttataa tacttctgga aatgttggct atcatgtatc ttttaacatg cgtaacttcc
      181 ctaataagaa agtctatgtc atgatgcggg caacgaatga tccagaggga aacactaaag
      241 gcggtgcgca agatttccat gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat
      301 agctcctcgt tgaggagcat tacataacta tattgacaac tataaaaatc attcgataaa
      361 atagtgatgt tatcgcatat cttcactatc acccataata gtcacactcc aagctatgcg
      421 ataacaggtt tgttgccaca cattctactg gttgattgtt tatggcttta tgtggcaaca
      481 accagtaccc ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt
      541 cgagtcctac agggtacatt aacgtagcca tttgaatcgt tctgtgttag aatttt
//
LOCUS       DAZKCN010000226          589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000226 DAZKCN010000000
VERSION     DAZKCN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.239"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>589
                     /locus_tag="ACG8K3_002747"
     CDS             <1..>589
                     /locus_tag="ACG8K3_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFP9146529.1"
                     /translation="AIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRK
                     KQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFN
                     IVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVD
                     NLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEY"
BASE COUNT          228 a           82 c           94 g          185 t
ORIGIN      
        1 gcgattataa ctgatttacc agattatatt aaaagaattg actccgattc ttttttagct
       61 gccttttatt tatatacagc taagtattgg atagacgata gtcggaaaac gattaagtat
      121 gcaaaaagga aaaaacaact ttattttcaa acatggcacg gaacgccttt aaaaaaaatt
      181 gaatttgatg caaaagataa gttacctaaa cgttacctaa gctatgcgaa aaaagatagt
      241 gagtcaatta catatttatt atctggtaat cgctatagtt ctaagaagta tgtttcagca
      301 ttcaatatcg ttccttctaa aatattagaa gtaggaactc ctagaaacga tgaattagcc
      361 tctagtaaag agcaagtgtt tgacttgaaa aagaaacaaa tcaatgtctt atttgctcca
      421 acttttcgaa ataatattga ggacaacgga attacgcaac tagaatggtt aggtgtggat
      481 aacttacgtg aattttttaa aaagcaacaa caagaattaa attttatgac taaatttcat
      541 cctaatgttc atagtaaatt agcaactgat tctaaaagta tagaatata
//
LOCUS       DAZKCN010000227          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000227 DAZKCN010000000
VERSION     DAZKCN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.240"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8K3_002748"
     tRNA            complement(65..150)
                     /locus_tag="ACG8K3_002748"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8K3_002749"
     tRNA            complement(167..238)
                     /locus_tag="ACG8K3_002749"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8K3_002750"
     tRNA            complement(250..322)
                     /locus_tag="ACG8K3_002750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8K3_002751"
     tRNA            complement(340..421)
                     /locus_tag="ACG8K3_002751"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8K3_002752"
     tRNA            complement(429..501)
                     /locus_tag="ACG8K3_002752"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8K3_002753"
     tRNA            complement(505..577)
                     /locus_tag="ACG8K3_002753"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZKCN010000228          580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000228 DAZKCN010000000
VERSION     DAZKCN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.241"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..306
                     /locus_tag="ACG8K3_002754"
     CDS             <1..306
                     /locus_tag="ACG8K3_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFP9146530.1"
                     /translation="EEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQL
                     SELPIISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            303..>580
                     /locus_tag="ACG8K3_002755"
     CDS             303..>580
                     /locus_tag="ACG8K3_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP9146531.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIG"
BASE COUNT          205 a           68 c           98 g          209 t
ORIGIN      
        1 gaagagatag atgttttaat cacagattat tcatctattt ttttcgattt tgcgctaaca
       61 aaaaaaccaa ttattctttt taactatgat gaaaaagaat atagcttaga aagaggtttt
      121 tatatacagt taagtgagtt accaattata agcgtacaag atagttatgg ccttgttgat
      181 gtttttaaaa accaacaaga aaagcttttg aaaagttctc aagagttact tgattatatt
      241 ggaaattatg aaacaggagc aagtacagaa aaggttcttg agttgataga gagggatata
      301 aagtgaaaaa aacagtaaaa gatatatttt ataatgcgat atatcaagtc tttttgattg
      361 ttttaccctt acttactata cctatactta gtcgtagaat tggttcaact ggtttaggga
      421 tttatgggta tgttttctca atatcacagt ttttaatgac tgtcattgct gtgggaatga
      481 atccttttcg tattcgaaat atcgctaaat caaggaaaga taaaaaagca ctatctttac
      541 aattctggaa tatttacttt attcaatttt taataggctt
//
LOCUS       DAZKCN010000229          566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000229 DAZKCN010000000
VERSION     DAZKCN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.242"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>566
                     /locus_tag="ACG8K3_002756"
     CDS             <1..>566
                     /locus_tag="ACG8K3_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFP9146532.1"
                     /translation="YFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVG
                     QKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLK
                     EAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPY
                     TNGKIEAKNTHIKTMKRVSYGFKSFENM"
BASE COUNT          196 a          116 c          106 g          148 t
ORIGIN      
        1 cctacttcca gcttaccaaa cgtattctgc caaatgcgaa agtagtgatt gatcggtttc
       61 atattatcaa acacatgaat caagccttta atgagttgcg tatccgtgaa atgaatgaac
      121 ttcgtaaagt aggacagaaa agtcaggcag aaaaactgaa aaagaactgg cgctttttgc
      181 taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt tttcgagcac
      241 caaaataccc atttcttacg gaagcgatga tgattgatcg attgcttggc ttttctacgt
      301 ccctgaagga ggcgtatccc tattttcatg aattagttga agcctttcga gacaaagacc
      361 ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac ggctttcggg
      421 aaaagctcca aaaccttctg acctatgaag aaggcatcac caacgcgatg atctatcctt
      481 acaccaatgg aaaaatagaa gcgaagaata cccatataaa gacaatgaaa cgagtatcct
      541 acggatttaa atcatttgag aacatg
//
LOCUS       DAZKCN010000230          564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000230 DAZKCN010000000
VERSION     DAZKCN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.243"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..498)
                     /locus_tag="ACG8K3_002757"
     CDS             complement(<1..498)
                     /locus_tag="ACG8K3_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146533.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPA"
BASE COUNT          189 a           87 c           81 g          207 t
ORIGIN      
        1 tgctggatag ttttctctaa aaaaagaaac atcaagtgga aattcatcag ctttattaat
       61 agtgtaatgc aaaagtttaa aatgtatttt ggttaactct cttcggatca attggctcgt
      121 ctcttctcgc aacagattaa actgattttc aaaactttca atgaatcgtt cattcaacca
      181 ttcgattgtt tcatcttcat tttttctcaa gtatttttga tccaatttat catgaagata
      241 aagccaagag agtgtcttat tgataaaaat aacggatttt tctcctaata ctccttttaa
      301 gtggtaaaat cctatatccg aaaaaaatag cgactgattg ctagcaatat ctaaagaatc
      361 agcttgttca gccaacaggt tgattgctaa aaagtggaat aatttctttt tttcataagc
      421 agacagagga taatactgtt ttaaatcttt taacattttt tctgttttta taattatagg
      481 tagcgtatac aattccacaa aacttatctc tgttgagtta aaaagattat ataaaaaaag
      541 ccgaatgttt ttaggattcc ctgt
//
LOCUS       DAZKCN010000231          538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000231 DAZKCN010000000
VERSION     DAZKCN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.244"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>538
                     /locus_tag="ACG8K3_002758"
     CDS             <1..>538
                     /locus_tag="ACG8K3_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFP9146534.1"
                     /translation="PDVIIGRKERSVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGH
                     QEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITI
                     KPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGT
                     PSQRPLNENSNGILRRNGL"
BASE COUNT          173 a          106 c          101 g          158 t
ORIGIN      
        1 gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga gaacacttta
       61 tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag gtaaaagaaa
      121 acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa tccatgatcg
      181 acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg atacgatcgt
      241 tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta aagtcattat
      301 cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta atcaatggtt
      361 ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa aagaattttc
      421 taactggaaa gccattagta accaacatga tattgatata tattttgcgg accctggaac
      481 accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta atggactg
//
LOCUS       DAZKCN010000232          522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000232 DAZKCN010000000
VERSION     DAZKCN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.246"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..412
                     /locus_tag="ACG8K3_002759"
     CDS             152..412
                     /locus_tag="ACG8K3_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146535.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          179 a           61 c           71 g          211 t
ORIGIN      
        1 aagtcctctt ttctgtgaat tggttgtcgt taactttatt ctacagaagc gacttccttt
       61 tttgtatgga ttttttcatt tacacaaaat attttacact ctcaattatt ttaatataag
      121 tacttcagat ttaataaagg agaaatatct aatgaaaaaa attgatttaa taaatatgat
      181 aggtatgtta atagggatat tagtaaatat agtaattttc acagattggc tcggggtgtt
      241 attttctaat cttattccca tacttattat aggcatttgt ggaattatac tttcaatatt
      301 agaacttttt gaaagcagaa atacaatgaa tagaatattt gcttgtatta tattaattgt
      361 taatctattg cctatggtct attttacttt tttatatttt gcactgggat gaaataaatt
      421 aaccaaaagt ttctttttct tataatgtgt atcaatatta aaactgaaaa agatatggat
      481 aataggatca aagccctcat tggaacactc aatatattat ca
//
LOCUS       DAZKCN010000233          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000233 DAZKCN010000000
VERSION     DAZKCN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.247"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..469
                     /locus_tag="ACG8K3_002760"
     CDS             <1..469
                     /locus_tag="ACG8K3_002760"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP9146536.1"
                     /translation="SIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQLE
                     ELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDP
                     LFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          185 a           98 c           77 g          160 t
ORIGIN      
        1 gtctatttat aattttgaaa ctactttaga agacggcact gtctattcat tgaaaaaata
       61 tgcaggcaaa ccgataatca ttgttaacac tgcaacaaaa tgcggtctag cacctcaatt
      121 taaacaatta gaagaacttt accaagctta tcagacacaa ggtctggtta tcttaggctt
      181 tccttccaat caatttaaac aagagctaga ttcagcgact gaagcagcag aagcttgtaa
      241 aaccacttat ggcgttacct tccctatgca tcaattgatt gacgtaaatg gagaaaatac
      301 agatcctctt tttacttatc ttaaaaatga agctcctggt actgtcacta aagcaataaa
      361 atggaatttt actaaatttt tgattgatag aaatggcaaa gtaatcgaac gatttgcacc
      421 acaaacttct ccattgaaaa tgaccgctgc aattgaatcc gttctttaac ttaataaaaa
      481 taaaaaaaga tgaactattt ctagttcatc tttttgcctt
//
LOCUS       DAZKCN010000234          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000234 DAZKCN010000000
VERSION     DAZKCN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.248"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8K3_002761"
     CDS             complement(<1..132)
                     /locus_tag="ACG8K3_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9146537.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKCN010000235          511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000235 DAZKCN010000000
VERSION     DAZKCN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.249"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..>511)
                     /locus_tag="ACG8K3_002762"
     CDS             complement(315..>511)
                     /locus_tag="ACG8K3_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9146538.1"
                     /translation="DNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERI
                     KQKLNCQSPVQFRKTTVTVV"
BASE COUNT          180 a           78 c           74 g          179 t
ORIGIN      
        1 aagtatttaa cgtcaaatta attttttaat gtctataaaa tccgttagaa aatacaattt
       61 gtagatatcc aattgatttg taaacaaaaa ggagttgtca actaagccac tcctttttgt
      121 tgtaattttt cccaataaga aagtctacta ctttaggaag ataatagtaa gatagaaatg
      181 ttgcccccaa aattattatc cagctggata taattaatca aataaaaagg atcaaaaatg
      241 tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcctt ttttcatgct
      301 ctaaaatgat atttttacac aaccgtaacg gttgttttac gaaattggac gggactttga
      361 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
      421 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
      481 ccaaaaaaat tctccataag tgaattatct a
//
LOCUS       DAZKCN010000236          509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000236 DAZKCN010000000
VERSION     DAZKCN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.250"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..>509
                     /locus_tag="ACG8K3_002763"
     CDS             81..>509
                     /locus_tag="ACG8K3_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146539.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQ"
BASE COUNT          193 a           93 c           91 g          132 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacag
//
LOCUS       DAZKCN010000237          502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000237 DAZKCN010000000
VERSION     DAZKCN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.251"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..130)
                     /locus_tag="ACG8K3_002764"
     rRNA            complement(<1..130)
                     /locus_tag="ACG8K3_002764"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(423..495)
                     /locus_tag="ACG8K3_002765"
     tRNA            complement(423..495)
                     /locus_tag="ACG8K3_002765"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Ala,seq:tgc)
BASE COUNT          134 a          127 c           93 g          148 t
ORIGIN      
        1 tgggttcccc cattcggaaa tctctggatc atagcttacg tacagctccc caaagcatat
       61 cggtgttagt cccgtccttc atcggctcct agtgccaagg catccaccgt gcgcccttat
      121 tcacttaacc ttatcaacct tgcggttggg ttttgatcct tcttctagcg atagaagtta
      181 gatcaagaaa ataagcaatt gaacttatta aaaaactcta tccagctcac aaatcagcct
      241 gtgcggcaaa aagatcgatc gtctcataaa atcaaagatt ttatggccgt ccgcctattt
      301 tgcttcaggc taaacaattt gttcagcttt caaattcaac gcggtgttct cggtttgtat
      361 tacttacatt tacttcaaat atccagtttt caatgaacaa atttaacaac taatgttgtt
      421 aatggagcct agcgggatcg aaccgctgac ctcctgcgtg caaagcaggc gctctcccag
      481 ctgagctaag gccccgtttt aa
//
LOCUS       DAZKCN010000238          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000238 DAZKCN010000000
VERSION     DAZKCN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.252"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..471)
                     /locus_tag="ACG8K3_002766"
     CDS             complement(<1..471)
                     /locus_tag="ACG8K3_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP9146540.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLP"
BASE COUNT          132 a           98 c           92 g          161 t
ORIGIN      
        1 cggaaggaag ttcatacgtg actggtaatg cttcgttgct tcacgcaaga tccgtgtcac
       61 cgtcccatcc gagacgaagt acttcttagc gattagttta cgtgattgat tttcgtacag
      121 ttccatgaga attgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc
      181 cgttttcgca gaaaaggtcc ttccacaatc atggcagaga aaacgggtac gttttaattc
      241 taaaatagtt aacttttctt tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt
      301 gcggataata cgaaccgatc gacagtgttc acaataggaa ggagagtcat ccaattttcc
      361 tttaataaac tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa
      421 aaataaatgt ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact
      481 cct
//
LOCUS       DAZKCN010000239          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000239 DAZKCN010000000
VERSION     DAZKCN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.253"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..143)
                     /locus_tag="ACG8K3_002767"
     CDS             complement(<1..143)
                     /locus_tag="ACG8K3_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9146541.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQ"
     gene            complement(154..360)
                     /locus_tag="ACG8K3_002768"
     CDS             complement(154..360)
                     /locus_tag="ACG8K3_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP9146542.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          137 a          107 c           66 g          172 t
ORIGIN      
        1 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
       61 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      121 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      181 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      241 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      301 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      361 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      421 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      481 ct
//
LOCUS       DAZKCN010000240          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000240 DAZKCN010000000
VERSION     DAZKCN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.255"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8K3_002769"
     CDS             complement(<1..430)
                     /locus_tag="ACG8K3_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9146543.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZKCN010000241          458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000241 DAZKCN010000000
VERSION     DAZKCN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.256"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..458)
                     /locus_tag="ACG8K3_002770"
                     /pseudo
     CDS             complement(106..458)
                     /locus_tag="ACG8K3_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          141 a           78 c           76 g          163 t
ORIGIN      
        1 aaattcttgc tcgaaagtta tggaaatttc gatagccgta ggctacttgt attgtgcaat
       61 agttttttac aaaaaaaagg ggtgcggata tttttttgga cctttttaac aaatatatct
      121 ttgcttcttc cgatattgaa gagggcttag ccctcctaaa gataacttaa ttcgttgatt
      181 attataccaa tgaatataat tatccaattt gtttataaac tgattgattg ttgtattctt
      241 ccagtcacgt tgataaaaca tctcgttttt taatcgtcca aagaaacctt cgcacgctga
      301 attatctggt gaataaccct ttttagacat agagtgaagc aagttgtctt tgttccaata
      361 gtccaactaa caatagcacc atcgtaacaa tcgacaagtg gtgataagta gattttccct
      421 gcgggaattc taaattcagt aatatctgtt aaccattt
//
LOCUS       DAZKCN010000242          456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000242 DAZKCN010000000
VERSION     DAZKCN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.257"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          156 a           55 c           66 g          179 t
ORIGIN      
        1 taaatatata aattagttaa tgtgttgtga ttgattattt aatatagata taatacaaaa
       61 aggcaaagat attttcaaaa cattttttct tgatgtgata tccttgtctt ttttgtaatg
      121 tttttaactt attgcttttg caaaatttat ttacctgttt tctggatcaa cgatagtgta
      181 tattatgcta tccttgtttg gattttagga cgtttaaaga ggcacttttt tacataactt
      241 aatccataga ccttttaagc atgctgtgag ataataataa agaagagttt aaagcgcacc
      301 ccaaaccact tccccataag tgtattacgc tttaaactct tttatatttg aattcactaa
      361 gaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttataattag gatttagagt
      421 aattaattag atgtttattt tataggactg atacta
//
LOCUS       DAZKCN010000243          451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000243 DAZKCN010000000
VERSION     DAZKCN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.258"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..>451
                     /locus_tag="ACG8K3_002771"
     CDS             249..>451
                     /locus_tag="ACG8K3_002771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146544.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWAYQMKAYRNKLKEYKIF"
BASE COUNT          135 a          105 c           85 g          126 t
ORIGIN      
        1 ataaataact cataaaaaat attttgactt tcaaaatcac ttttgctacc agaagttgag
       61 gcgaagtgtt ttaagcatag ttgtacccta gtttgaccgt taaaaataaa taacgatcgc
      121 tagagtgcaa ctatgtttgt ttgctctacc gtcagcgtcg gcttggcctc tcacatccat
      181 taccgtcggg atcatctccg ttacataccc ttacaaaata cgccctgtaa gcattgcatg
      241 aaaccaccgt gcatttgcac cgtatttccg ccctagccgt tcaaatcggt cgctaccacc
      301 attcagaaac acgtgcggta atggaaggtt tggaggaagc tatccagacc actaacgatt
      361 gtccctatcg tcgtacgttt cactcagatc aaggttgggc gtatcaaatg aaagcttata
      421 gaaacaaatt gaaggagtac aaaatctttc a
//
LOCUS       DAZKCN010000244          446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000244 DAZKCN010000000
VERSION     DAZKCN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.259"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>446)
                     /locus_tag="ACG8K3_002772"
     CDS             complement(39..>446)
                     /locus_tag="ACG8K3_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146545.1"
                     /translation="ELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQ
                     FIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNL
                     TKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           82 c           73 g          154 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactc
//
LOCUS       DAZKCN010000245          444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000245 DAZKCN010000000
VERSION     DAZKCN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.260"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8K3_002773"
     CDS             <1..379
                     /locus_tag="ACG8K3_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146546.1"
                     /translation="HRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTE
                     GFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          141 a           93 c           80 g          130 t
ORIGIN      
        1 tcatcgactg tttaaacgac caattagtca aacagaaatc gttgatgagc tgttaagcta
       61 caacgaagaa ttgcgaactg cgtatcattt ttgtcaatta ttacgctatt atttcgttaa
      121 acgaggtacg gaaggctttc aagaaaccct taaaacaatt tcatctacgt tacctcagtc
      181 gtttaaaaag aaattcacga tcttccatcg ctaccaatcg ggtatttcaa atgcctttaa
      241 ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc tgattaaacg
      301 aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt tacaacaagg
      361 tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat atcggactaa
      421 gttttattca aatttgtctt cacc
//
LOCUS       DAZKCN010000246          424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000246 DAZKCN010000000
VERSION     DAZKCN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.261"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..>424)
                     /locus_tag="ACG8K3_002774"
     CDS             complement(14..>424)
                     /locus_tag="ACG8K3_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9146547.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
BASE COUNT          139 a           81 c           71 g          133 t
ORIGIN      
        1 aacataaaac gcactaagtt attttattga acatatatct tactttatct atccgactat
       61 ttagacgacg ggtctggcaa acaggttcgc cagtggtaac ctgatatcct tttagctctg
      121 ctaaacaaac actaagccca tttgtaaaaa aagttaaatc attgcgataa tcttgaatac
      181 atcgagcagg aatttctcca ataataatga cctcattatt tttcagttga gtatttacga
      241 tatttgcaca atatttggga gcatcgttat atgcccgtga aagatattcc tgtggtgcat
      301 aaactttaaa actaagatat ggctctaaca attctgttcc agcttttcta aaggcttgct
      361 ccagtacaat aggagtaagc atccgaaaat ctgctggagt actaacaggg ctatagtata
      421 aacc
//
LOCUS       DAZKCN010000247          422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000247 DAZKCN010000000
VERSION     DAZKCN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.262"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..>422
                     /locus_tag="ACG8K3_002775"
     CDS             241..>422
                     /locus_tag="ACG8K3_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP9146548.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT          145 a           75 c           62 g          140 t
ORIGIN      
        1 tatagagaaa aatgcaggtt aatactttct taaataaata tttatggtga acaaaaacaa
       61 aaaagcaagt tttattaaac gtaacccttt tcatttcata gattactgta tttattacaa
      121 aaaacgcgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat tttcacgtgt
      181 taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag gagatttttc
      241 atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc tgcattcttc
      301 atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga tactctatca
      361 acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc agaatcaaat
      421 tc
//
LOCUS       DAZKCN010000248          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000248 DAZKCN010000000
VERSION     DAZKCN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.263"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..>419
                     /locus_tag="ACG8K3_002776"
     CDS             75..>419
                     /locus_tag="ACG8K3_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146549.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          186 a           57 c           61 g          115 t
ORIGIN      
        1 taaatatata aaaagccttc gggctttttc ttttaacaat taataggaac gtatattcga
       61 aaggaataga aataatgtgg ttagaaaaaa taaatgacaa aaaatttaag tacaccgaac
      121 gttatacgga tcccttaact gaaaaaaaga gaaaagtgtc cgttacttta tctagcaatt
      181 cacgccaagc ttggaatcaa gctaacttac ttttgaatga aaaaatcgca gaaaaaataa
      241 aacgaaatga agaattacca ctaacttttg gagaattaaa aactaaatgg gatgaaaaat
      301 acaagccaac tgtaaaagaa agttcctatc gaacaactca ggtttatcta tcattaattt
      361 caaaatatat aaaagatgat gtacttgtaa aaaacgttaa tagtaatctt atacaagat
//
LOCUS       DAZKCN010000249          401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000249 DAZKCN010000000
VERSION     DAZKCN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.264"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..>401
                     /locus_tag="ACG8K3_002777"
     CDS             60..>401
                     /locus_tag="ACG8K3_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9146550.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDG"
BASE COUNT          149 a           58 c           79 g          115 t
ORIGIN      
        1 ttctattctt acgtggtatc tttgaataaa gcgaatcaat aagaaagaga tggaagcaga
       61 tgctaacgaa aaaagaacta atcgatcaca tcaatgaaac atacaatttg tctccagaat
      121 atatttttaa aaaatttcct gattattgcg tattcagaca tcaaaaaagc aaaaaatggt
      181 ttggggtcct gatgtctgtt ccagaagaaa aaatctatga tgatgcaagt gaccatagga
      241 tcatcaatat tcttgatttg aaggtcgatc ctgaacttgg cggaattttg cgaggaaaag
      301 aggggtattt tccagcctat cacatgaata aagaacattg gatctctatt gatctacgta
      361 aaatagaaaa gctagatgaa ctagcagatt tgattgatgg a
//
LOCUS       DAZKCN010000250          396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000250 DAZKCN010000000
VERSION     DAZKCN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.265"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..202
                     /gene="tnpB"
                     /locus_tag="ACG8K3_002778"
     CDS             <1..202
                     /gene="tnpB"
                     /locus_tag="ACG8K3_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9146551.1"
                     /translation="QDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVR
                     WLLEGLSIDQPRAILPGKKGVF"
BASE COUNT          130 a           73 c           83 g          110 t
ORIGIN      
        1 tcaagaccgt ttcaaacttc tctattggga cggtgatggt tttcttctct gttataagcg
       61 gatagaaaac ggcaagttaa aatggcctcg gacgaaagac gaagttcgaa ccttaacgaa
      121 ccaacaggtt cgatggttgt tagaaggatt aagtatcgat caacctcgag ctattttgcc
      181 aggaaaaaaa ggagtttttt aagctggggg gttaacacaa aaaatgtgtt gaacccctat
      241 ttcttgtgcg tttcttgtgg tattctgtaa caaaatcagt tcgattcaag gtggtgaaaa
      301 attttggaaa ctacagatac gttgcttcag ttgcttcaag aagcccataa aaccaatcaa
      361 gcacaacaac aaaccattca gaatcttaca actgaa
//
LOCUS       DAZKCN010000251          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000251 DAZKCN010000000
VERSION     DAZKCN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.266"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..>371
                     /locus_tag="ACG8K3_002779"
     CDS             128..>371
                     /locus_tag="ACG8K3_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146552.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT          151 a           54 c           62 g          104 t
ORIGIN      
        1 tattttttta acttattgtt taagagtgat acgcgaattg taaaattaag ttagaaaaat
       61 aaaaaggcat ttatggtaca ctcaaattgt atttccgacg aaagaaaaca aaggagtgta
      121 accataaatg acctacaaac atcttaccat agacgaactg acaatgatag aatcatatta
      181 tcttcaacat aataagccgg ttgaaatcgc taaccgaatg ggacgtgcta tacaaactat
      241 ttataatgta gtcaataagt tcaagcaagg caagactgct cttgattatt ggcaccagta
      301 taaagaaaat aagaaaaaat gtggtagaaa agtcattcaa ttacctgctc atgaagtaga
      361 ttacattaaa g
//
LOCUS       DAZKCN010000252          364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000252 DAZKCN010000000
VERSION     DAZKCN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.267"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>364
                     /locus_tag="ACG8K3_002780"
     CDS             <1..>364
                     /locus_tag="ACG8K3_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9146553.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHT"
BASE COUNT          132 a           62 c           68 g          102 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atac
//
LOCUS       DAZKCN010000253          356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000253 DAZKCN010000000
VERSION     DAZKCN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.268"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>356
                     /locus_tag="ACG8K3_002781"
     CDS             <1..>356
                     /locus_tag="ACG8K3_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9146554.1"
                     /translation="KKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKVS
                     LSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICA
                     DGETGQLIDVLPTRKL"
BASE COUNT          114 a           68 c           66 g          108 t
ORIGIN      
        1 aaaaaagcag cgctatacct gtaaaaactg tcgaacccat tggacggctc aaagttattt
       61 tgttcaaccc agacattcaa tcgcgaatca tgtcagatat aaaattgctt ctttactgac
      121 tgaaaaagta tctttatctt ttattgcgaa aaattgtcag gtatctttga ccaccgttat
      181 tcgtacgttg aaagagttta aaagctactt accaaagcaa tctaagagga ttctccccag
      241 agtattgatg gttgatgaat ttcgttcgca cgcttccata gaagataaaa tgagctttat
      301 ttgcgcagat ggcgaaacag gacaattaat tgatgtcttg cctacgcgta aattgc
//
LOCUS       DAZKCN010000254          355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000254 DAZKCN010000000
VERSION     DAZKCN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.269"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..251
                     /locus_tag="ACG8K3_002782"
     CDS             57..251
                     /locus_tag="ACG8K3_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146555.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            241..>355
                     /gene="tnpB"
                     /locus_tag="ACG8K3_002783"
     CDS             241..>355
                     /gene="tnpB"
                     /locus_tag="ACG8K3_002783"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9146556.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLE"
BASE COUNT          124 a           59 c           69 g          103 t
ORIGIN      
        1 actaacaaat gcacctcaag atatacttga gctatcaaga agtctggagg ttttctatgt
       61 ataaattaaa tgagattgtt cctgtccaat taggaaacga aaagagtgca ccagaaaaag
      121 gtaaacccgc ggctgccaag ttagcgatgc gcatcaaaag agagaatggt tctgaagttt
      181 tgatctataa cggtattaac aattatattc ttcaagctat tttgaaagaa ctgttgtcca
      241 atgctttatg atttacttca aaccaaacat atttatattg tgtgcggaaa aacagatttg
      301 cgaaaaggaa ttgatggtct tgccagtctt attcaacaag agtatcaact cgagc
//
LOCUS       DAZKCN010000255          349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000255 DAZKCN010000000
VERSION     DAZKCN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.270"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..>349)
                     /locus_tag="ACG8K3_002784"
     CDS             complement(162..>349)
                     /locus_tag="ACG8K3_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HFP9146557.1"
                     /translation="MLLICVDVAKYLKDIELVRKTLTIPQWADKLGKRAGINFSVLLT
                     ESIADKADNILRSGRNN"
BASE COUNT          116 a           62 c           56 g          115 t
ORIGIN      
        1 tcatttacat tcaatagaac accaagagcc caacagggta tactctggtg tccttttaaa
       61 aaagtaactt tctgacctaa ttaattgagc tactaaaaaa gctatgaaat gaggaataaa
      121 aaagggatca aaaactttta agtttctgac ccttttgagt tctaattatt tcttccagaa
      181 cgaagaatgt tatctgcttt atctgcaatt gattctgtta gaaggactga aaaatttatg
      241 ccagcccttt tgcctagctt atccgcccat tgaggaattg ttaaggtttt tctgacaagt
      301 tctatgtctt tcaaatattt ggcaacatct acacagatta aaagcatga
//
LOCUS       DAZKCN010000256          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000256 DAZKCN010000000
VERSION     DAZKCN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.272"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..>333
                     /locus_tag="ACG8K3_002785"
     CDS             94..>333
                     /locus_tag="ACG8K3_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP9146558.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRT"
BASE COUNT          109 a           63 c           58 g          103 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc act
//
LOCUS       DAZKCN010000257          332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000257 DAZKCN010000000
VERSION     DAZKCN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.273"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>332
                     /locus_tag="ACG8K3_002786"
     CDS             <1..>332
                     /locus_tag="ACG8K3_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146559.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAZKCN010000258          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000258 DAZKCN010000000
VERSION     DAZKCN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.274"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>323)
                     /locus_tag="ACG8K3_002787"
     CDS             complement(34..>323)
                     /locus_tag="ACG8K3_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146560.1"
                     /translation="TAVSRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFST
                     VKELNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT           96 a           62 c           39 g          126 t
ORIGIN      
        1 aaaaaaataa caaacaaact cgcttgaaat tcattaggtg agtttgttta ttattttttc
       61 tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg gtttcattag gaatatagtc
      121 agcacatttt cttttctcta acattctata cccactatta tgtattccta aggtattcaa
      181 ttccttaact gttgaaaaca aattccaaac ggtttggtgg cttccttttc cgctacttcc
      241 attataaata atatcataat tttctttcaa atactcatca gattttttaa aagcactgta
      301 gtatgctctt gaaacagcag ttc
//
LOCUS       DAZKCN010000259          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000259 DAZKCN010000000
VERSION     DAZKCN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.275"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8K3_002788"
     CDS             <1..>321
                     /locus_tag="ACG8K3_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146561.1"
                     /translation="KNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFM
                     IQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFT"
BASE COUNT          117 a           62 c           60 g           82 t
ORIGIN      
        1 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
       61 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      121 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      181 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      241 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      301 tcctaagaaa tatctttacc a
//
LOCUS       DAZKCN010000260          318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000260 DAZKCN010000000
VERSION     DAZKCN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.276"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..124
                     /locus_tag="ACG8K3_002789"
     CDS             <1..124
                     /locus_tag="ACG8K3_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP9146562.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          118 a           52 c           38 g          110 t
ORIGIN      
        1 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
       61 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
      121 ttgacaaatc ataactttaa attacggatc atgatttggc atggggaatc attactaata
      181 aaataaaaaa atgaacagaa agaaaacagt tctttctgtt caccaacttc atttgacaaa
      241 tatcctattt agcttatttt atagcttttt atatacactt ttttaaaaag tgatttttcc
      301 acatgaattc ccatgagc
//
LOCUS       DAZKCN010000261          293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000261 DAZKCN010000000
VERSION     DAZKCN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.277"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>293)
                     /locus_tag="ACG8K3_002790"
     CDS             complement(<1..>293)
                     /locus_tag="ACG8K3_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146563.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYL"
BASE COUNT          111 a           49 c           36 g           97 t
ORIGIN      
        1 atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac ttgttcttga
       61 ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt tatttcttct
      121 cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac tggccactga
      181 tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg tattaaacag
      241 tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DAZKCN010000262          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000262 DAZKCN010000000
VERSION     DAZKCN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.278"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>292
                     /locus_tag="ACG8K3_002791"
     CDS             <1..>292
                     /locus_tag="ACG8K3_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146564.1"
                     /translation="RTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNIS
                     TSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA"
BASE COUNT           84 a           61 c           58 g           89 t
ORIGIN      
        1 aaagaacatt cgtagcggat acctctgttg ctgaaaaaca ctgtttcatt agtcaaaaag
       61 ttcgttggtc tgtcattgct cgcttaaaag agaatacttc catgacggaa atcgctcgtc
      121 agaaaaatat ttccacttct tcggtctatc gtgtcatgaa gcgattctac cgcccattga
      181 atcctttcaa acagactttg ccaaaggtct tatgctttga tgaattcaaa tcggttcgaa
      241 gtgttgccag tgcaatgagt tttatcatga tggacggaca aagccatgca tt
//
LOCUS       DAZKCN010000263          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000263 DAZKCN010000000
VERSION     DAZKCN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.280"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>264
                     /locus_tag="ACG8K3_002792"
     CDS             <1..>264
                     /locus_tag="ACG8K3_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146565.1"
                     /translation="GIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQ
                     YTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRL"
BASE COUNT           99 a           53 c           44 g           68 t
ORIGIN      
        1 aggaattttt aagttcacag atattaactt agcgcgtgag gctaaaaatc gattgattca
       61 tgattatatc gatcaaccaa aatattcaaa agcttgcgca tcattggatg atggattcga
      121 agacgccttt caatataccg tacaaggaaa ttcccacaat cgactaaaga gtaccaatct
      181 aattgaacga ctgaatcaag aagtacgcag aagagaaaag attattcgca tcttccccaa
      241 tcaaacatca gccaatcgct taat
//
LOCUS       DAZKCN010000264          236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000264 DAZKCN010000000
VERSION     DAZKCN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.282"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>236
                     /locus_tag="ACG8K3_002793"
     CDS             <1..>236
                     /locus_tag="ACG8K3_002793"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP9146566.1"
                     /translation="NETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYG
                     AGSYANANSPVQIELQHTHDKATFEKNYKAYVELA"
BASE COUNT           74 a           45 c           58 g           59 t
ORIGIN      
        1 aatgaaacag caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct
       61 tatactgctt atattgtggg agacggcgga attgtttatc aagtcggtca acctggttat
      121 gtacagtacg gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa
      181 cacacacatg ataaagcaac gtttgagaaa aactacaagg catacgttga attggc
//
LOCUS       DAZKCN010000265          227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000265 DAZKCN010000000
VERSION     DAZKCN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.283"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..227
                     /locus_tag="ACG8K3_002794"
                     /pseudo
     CDS             1..227
                     /locus_tag="ACG8K3_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DAZKCN010000266          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000266 DAZKCN010000000
VERSION     DAZKCN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.284"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG8K3_002795"
     CDS             <1..>224
                     /locus_tag="ACG8K3_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9146567.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKCN010000267          210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000267 DAZKCN010000000
VERSION     DAZKCN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.285"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>210)
                     /locus_tag="ACG8K3_002796"
     CDS             complement(<1..>210)
                     /locus_tag="ACG8K3_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP9146568.1"
                     /translation="TQEQPLEAIVTDLTYVRVRKKWHYICLILDLFNREIIGYSCGEK
                     KDASLVKEAFGRIPYSLTDVKLFHT"
BASE COUNT           67 a           45 c           35 g           63 t
ORIGIN      
        1 ctgtatgaaa aagcttgacg tctgttaaag aatacggtat ccgtccaaag gcttctttta
       61 ccaatgaggc atctttcttc tcaccacagg aataaccaat aatttctcga ttaaacaaat
      121 caagtattaa gcagatataa tgccactttt tcctcacgcg aacataagta agatccgtaa
      181 cgatggcttc caatggctgt tcttgtgtaa
//
LOCUS       DAZKCN010000268          205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000268 DAZKCN010000000
VERSION     DAZKCN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.denovo.287"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>205
                     /locus_tag="ACG8K3_002797"
     CDS             <1..>205
                     /locus_tag="ACG8K3_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9146569.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DAZKCN010000269        23915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.040, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000269 DAZKCN010000000
VERSION     DAZKCN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.040"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            392..1009
                     /locus_tag="ACG8K3_002798"
     CDS             392..1009
                     /locus_tag="ACG8K3_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9146570.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1050..1349
                     /locus_tag="ACG8K3_002799"
     CDS             1050..1349
                     /locus_tag="ACG8K3_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP9146571.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1383..1550
                     /locus_tag="ACG8K3_002800"
     CDS             1383..1550
                     /locus_tag="ACG8K3_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9146572.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1595..2200)
                     /locus_tag="ACG8K3_002801"
                     /pseudo
     CDS             complement(1595..2200)
                     /locus_tag="ACG8K3_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2341..5307)
                     /locus_tag="ACG8K3_002802"
     CDS             complement(2341..5307)
                     /locus_tag="ACG8K3_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP9146573.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            5453..6028
                     /locus_tag="ACG8K3_002803"
     CDS             5453..6028
                     /locus_tag="ACG8K3_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9146574.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            6242..6937
                     /gene="vanR-A"
                     /locus_tag="ACG8K3_002804"
     CDS             6242..6937
                     /gene="vanR-A"
                     /locus_tag="ACG8K3_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP9146575.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            6915..8069
                     /locus_tag="ACG8K3_002805"
     CDS             6915..8069
                     /locus_tag="ACG8K3_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFP9146576.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            8284..9252
                     /locus_tag="ACG8K3_002806"
     CDS             8284..9252
                     /locus_tag="ACG8K3_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP9146577.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            9245..10276
                     /gene="vanA"
                     /locus_tag="ACG8K3_002807"
     CDS             9245..10276
                     /gene="vanA"
                     /locus_tag="ACG8K3_002807"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP9146578.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            10282..10890
                     /locus_tag="ACG8K3_002808"
     CDS             10282..10890
                     /locus_tag="ACG8K3_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFP9146579.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            11318..12229
                     /gene="vanY"
                     /locus_tag="ACG8K3_002809"
     CDS             11318..12229
                     /gene="vanY"
                     /locus_tag="ACG8K3_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP9146580.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYETWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            12382..12867
                     /gene="vanZ-A"
                     /locus_tag="ACG8K3_002810"
     CDS             12382..12867
                     /gene="vanZ-A"
                     /locus_tag="ACG8K3_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFP9146581.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            13181..16168
                     /locus_tag="ACG8K3_002811"
     CDS             13181..16168
                     /locus_tag="ACG8K3_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP9146582.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            16405..16629
                     /locus_tag="ACG8K3_002812"
     CDS             16405..16629
                     /locus_tag="ACG8K3_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9146583.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            16644..17513
                     /locus_tag="ACG8K3_002813"
     CDS             16644..17513
                     /locus_tag="ACG8K3_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9146584.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            17494..18228
                     /locus_tag="ACG8K3_002814"
     CDS             17494..18228
                     /locus_tag="ACG8K3_002814"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9146585.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            18261..19124
                     /locus_tag="ACG8K3_002815"
     CDS             18261..19124
                     /locus_tag="ACG8K3_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFP9146586.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            19168..19695
                     /locus_tag="ACG8K3_002816"
     CDS             19168..19695
                     /locus_tag="ACG8K3_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFP9146587.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            19923..21242
                     /locus_tag="ACG8K3_002817"
     CDS             19923..21242
                     /locus_tag="ACG8K3_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HFP9146588.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
     gene            21394..22920
                     /locus_tag="ACG8K3_002818"
     CDS             21394..22920
                     /locus_tag="ACG8K3_002818"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFP9146589.1"
                     /translation="MAICSKKQVKFRAMNYSGFFEVEASIKVNQGSFKGVLSPTQRFK
                     SQEELLSFVSSKQAKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKATASQ
                     TLKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPRKRY
                     VDGSKVQEHHAIIPTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEETKIQ
                     TKVADLLFQSIGKTPKQEGWKILFKQQTKEEKEDVQTLPLVIIGEHAEVDVKSAEKET
                     QPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQV
                     IKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFINNIKKFI
                     VHLLEAVPNDIEKLNFSDYQEQKEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECK
                     FTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK
                     "
     gene            23040..23279
                     /locus_tag="ACG8K3_002819"
     CDS             23040..23279
                     /locus_tag="ACG8K3_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP9146590.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDMMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            23328..23411
                     /locus_tag="ACG8K3_002820"
     CDS             23328..23411
                     /locus_tag="ACG8K3_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFP9146591.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            23536..23880
                     /locus_tag="ACG8K3_002821"
                     /pseudo
     CDS             23536..23880
                     /locus_tag="ACG8K3_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
BASE COUNT         7986 a         4026 c         4746 g         7157 t
ORIGIN      
        1 ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta gaaatatata gtgttgtaat
       61 agaataatag tgttaaaaaa attgtaaaag ttaaagaagt caaattctga tgtgaaaaat
      121 aaaatatacc atggaagaca taaaagaaaa actggataac tagtttattt aatggcagac
      181 cgttaaggcc tgccattttt atgtatttta gcggaataaa tataatcatg cgggagtgta
      241 aacttactgt ttcaaaaagt atactttttg acatgccttg attttctttg tttatgtatg
      301 gcataataac cacataatac aatttgaaac gtttctgatt taatctgatt gttttgaaac
      361 aattttagtg aaaaaagggt agataaaaac gatgagaaaa tttttttata tccgggtcag
      421 tagttatgat caatcgatcg aacgacaact gatttccgcg aaggaattag gaatccctga
      481 agaatatata tttgtagaaa aagccagcgg caaagatttt aaacgccctg aatatcaatt
      541 aatgaaacgg atgtttcgaa aaggagattt actctatcta caatcattag atagactagg
      601 cagaaataaa caaatgattt tagaagaatg gcgagaatta actaaggaaa agcagattga
      661 tatttctgtg ttagacatgc ctctgttaga tactgctagt tacaagcaac tagatgggtt
      721 agagacgctt atctctgact taattctgca attactcagc tatatggccg aagacgagcg
      781 gaaacgaatt aatagccggc aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa
      841 attcggcaga aagaaaattg ctttacctcc tgaatttcct gcgatctatt ctgactggaa
      901 agcaggaaag ataactgcag tctatgctat ggatcaattg ggcatgaaac gaaatacctt
      961 ttatcgacgg gtgaaagaat atgaacatac cttgcaccac ttttcttgat agatataaca
     1021 gctattagac gggaagggag agtttgaaaa tggacttaac gaatcaaagg gtcgttttta
     1081 ctggaacgct gcagcaaatg acacgaaccc aagccatcgg tttggttcac tctttaaacg
     1141 gccattgtca atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt cagtattcta
     1201 agtcgttgtt tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg
     1261 cgttaggtca tgagattacc attttatcag aagtcgaatt ttatgcgtta attagtcaac
     1321 agttgaaaga atggcaacgg tatttataat cgttagtaaa atttacaagg aggaaaattt
     1381 ttatgaagcg aaaatcttat gatcgttggg gcattgaaat aactagtttt aatcgaaata
     1441 ttgcttttca cgtggagtgt gactgtgggg aattagcaaa aagtgtttat caaaaaaatt
     1501 acttttactg tcctaaatgc catcgccgat acggggtaca tggaacatag aaacgaaata
     1561 gtgcggtaga aaagcgaatg aatgtagtat atttctacaa atcattcgct ttctttattt
     1621 tataaatagt tggtcgagag atcccgtttt cttttgcaat ctctgcaact ggaatgcctt
     1681 gctttaacat gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa
     1741 tagaatactc gattggtcgt cccttatatc ggccggcttt ttgagccaat aatactcctt
     1801 accgctgacg ttcacgaatt ttctcacgct cgttttgtgc aatataactt agcagtgata
     1861 aaaacatatc gaacatagcg gtatccagga gttcatttcc agtattaaaa tctaaaaact
     1921 gtgcatctaa tatttttaac gctacttttt tctgtttcat aaaagcaact gtatttttta
     1981 tatcttcata atctcttcct aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac
     2041 ggatataatt tagtaatttt tgaaactcag gacgctcagt atctttacca ctggcttttt
     2101 cttggaataa tttatcaatg ttaagtcctt ccatcattgc caattgtcga tccaaatttt
     2161 gatcttgtga agatactcga atatagccaa tttttcccat aaaaaactcc tttttaaaag
     2221 tgatataatt tacatgtaaa atttacttgt agttttactg ctatcagggg tagcgtcagg
     2281 aaaatgcgga tttacaacgc taagcctatt ttcctgacga atccctcgtt tttaacaacg
     2341 ttaagaaagt tttagtggtc ttaaagaatt taatgagact actttctctg agttaaaatg
     2401 gtattctcct agtaaattaa tatgttccca acctaagggc gacatatggt gtaacaaatc
     2461 ttcattaaag ctacctgtcc gttttttata ttcaactgct gttgttaggt ggagagtatt
     2521 ccaaatactt atagcattga taattatgtt taaagcactg gctctttgca attgatgctg
     2581 tatggtgcgt tctctaagct caccttgttt tccgaagaaa atagctcttg ccaatccatt
     2641 catggcttct cctttattca atcctctttg tatttttctt cttaatgatt catccgatat
     2701 ataattcaaa ataaagatcg ttttttctat tcggcccatc tcacgtaagg ctgtagctaa
     2761 gctgttttgt cttgaatagg aacctagctt ccccataata agggatgctg aaactgttcc
     2821 ctcccttata gaatgagcta atcgcaaaac atcctcataa ttttctttaa tgacctttgt
     2881 atttatttgt ccacgtaaaa tggcttctag ttttggatac tcacttgctt tatctatcgt
     2941 aaataatttt gagtccgata aatcccttat tcttggggca aatttaaatc ctaataaatg
     3001 agtcagtccg aatatttggt cagtgtaacc ggcagtgtct gtataatgtt cctctatgtt
     3061 tagatccgtc tcatgatgta acaaaccatc caaaacatga atcgcatctc ttgaattagt
     3121 atgaataatc tttgtgtagt aagaagagaa ttgatcactt gtaaatcggt agatggtggc
     3181 tccttttcca gttccataat gtggatttgc atctgcatgt agtgatgaaa cacctagctg
     3241 cattctcata ccatctgacg aagatgttgt accgtcgccc caatagaaag gcaattgtaa
     3301 tttatgatga aagtttacta atatggcttg ggctttattc atggcatctt catacatgcg
     3361 ccattgagat acattggcta gttgcttata tgtaagtccg ggtgtggctt cggccatctt
     3421 gctcaagcca atattcattc ccattcctaa aagggcagcc atgataatga ttgtttcttc
     3481 cttatctggt tttcgattat tggaagcatg agtgaattgc tcatgaaatc ctgttatatg
     3541 ggccacatcc atgagtaaat cagttaattt tattcttggt agcatctgat aaaggcttgc
     3601 actaaatttt tttgcttctt ctggaacatc tttttctaag cgtgcaagtg atagctttcc
     3661 tttttcaaga gaaaccccat ctaacttatt ggaattggca gctaaccact ttaacctttc
     3721 attaaagctg ctggttctct ccgttatata atcttcgaat gataaactaa ctgataatct
     3781 cgtattcccc ttcgattgat tccatgtatc ttccgaaaac aaatattcct caaaatccct
     3841 atattgtctg ctgccaacaa tggaaacatc tcctgcccga acatgctccc gaagttctgt
     3901 taaaacagcc atttcatagt aatgacgatt aattgttgta ccatcatcct cgtataaatg
     3961 tcttttccat cgttttgaaa taaaatccac aggtgagtca tcaggcactt ttcgctttcc
     4021 agattcgttc attcctcgga taatctcaac agcttgtaaa agtggctcat ttgcctttgt
     4081 agaatgaaat tccaatactc ttaatagcgt tggcgtatat tttcttagtg aataaaaccg
     4141 tttttgcagt aagtctaaat aatcatagtc ggcaggacgt gcaagttcct gagcctcttc
     4201 tactgaagag acaaaggtat tccattcaat aaccgattct aaaaccttaa aaacgtctaa
     4261 tttttcctct cttgctttaa ttaatgcttg tccgatgttc gtaaagtgta taactttctc
     4321 atttagcttt ttaccgtttt gtttctggat ttcctcttga gccttacgac cttttgataa
     4381 caaactaagt atttgcctat catgaatttc aaacgcttta tccgttagct cctgagtaag
     4441 ttgtaataaa tagatggtta atatcgaata acgtttattt tcttgaaagt cacggaatgc
     4501 atacggctcg tatcttgagc ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg
     4561 actaatttgc actgtttcta aatccattcc tcgtatgtat tcgagtcgtt ctattatttt
     4621 tagaaaagtt tcgggtgaag gatgacccgg tggctctttt aaccaaccca atatcgtttt
     4681 attggattcg gatggatgct gcgaggtaat aatcccttca agcttttctt tttgctcatt
     4741 tgttagagat ttactaaccg tattaaatag cttcttttca gccattgccc ttgcttccca
     4801 caccattctt tcaagtgtag tgatagcagg cagtataatt ttgttttttc ttagaaaatc
     4861 tatgcattca tgcagtagat gaatggcatc accattttcc aaagctaatt gatgaaggta
     4921 cttaaatgtc attcgatatt cactcagggt aaaagttaca aagtcgtatt cacttcgaat
     4981 ttctttcaaa tgatcccaaa gtgtattttc cctttgagga taatgatcaa gcgaggatgg
     5041 actaacacca atctgtttcg atatatattg tatgaccgaa tctgggatgc ttttgatatg
     5101 agtgtatggc caaccgggat accgaagaac agctaattga acagcaaatc ctaaacggtt
     5161 ttcttccctc cttcgcttat taactatttc taaatcccgt ttggaaaaag tgaagtaggt
     5221 ccccagtatc cattcatctt cagggatttg cataaaagcc tgtctctgtt ccggtgtaag
     5281 caattctcta cctctcgcaa ttttcattca gtatcattcc atttctgtat tttcaattta
     5341 ttagttcaat tatatatcaa tagagtgtac tctattgata caaatgtagt agactgataa
     5401 aatcatagtt aagagcgtct cataagactt gtctcaaaaa tgaggtgata ttttgcggaa
     5461 aatcggttat attcgtgtca gttcgactaa ccagaatcct tcaagacaat ttcagcagtt
     5521 gaacgagatc ggaatggata ttatatatga agagaaagtt tcaggagcaa caaaggatcg
     5581 cgagcaactt caaaaagtgt tagacgattt acaggaagat gacatcattt atgttacaga
     5641 cttaactcga atcactcgta gtacacaaga tctatttgaa ttaatcgata acatacgaga
     5701 taaaaaggca agtttaaaat cactaaaaga tacatggctt gatttatcag aagataatcc
     5761 atacagccaa ttcttaatta ctgtaatggc tggtgttaac caattagagc gagatcttat
     5821 tcggatgaga caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg
     5881 attaaagaag tatcataaaa atcacgcagg aatgaattat gcggtaaagc tatataaaga
     5941 aggaaatatg actgtaaatc aaatttgtga aattactaat gtatctaggg cttcattata
     6001 caggaaatta tcagaagtga ataattagcc attctgtatt ccgctaatgg gcaatatttt
     6061 taaagaagaa aaggaaacta taaaatatta acagcctcct agcgatgccg aaaagccctt
     6121 tgataaaaaa agaatcatca tcttaagaaa ttcttagtca tttattatgt aaatgcttat
     6181 aaattcggcc ctataatctg ataaattatt aagggcaaac ttatgtgaaa gggtgataac
     6241 tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt
     6301 atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga
     6361 atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag
     6421 cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac
     6481 cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat
     6541 aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata
     6601 caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt
     6661 cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac
     6721 cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct
     6781 gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt
     6841 gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa
     6901 aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag
     6961 aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt
     7021 atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca
     7081 aatatgactt aaatcacctg gacgcgatga aattatatca atattccata cggaacaata
     7141 tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc
     7201 tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga
     7261 acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca
     7321 cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa
     7381 atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt
     7441 atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc
     7501 atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta
     7561 cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc
     7621 tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta
     7681 ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta
     7741 acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg
     7801 cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag
     7861 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
     7921 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
     7981 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
     8041 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttttag gaaaatctca
     8101 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
     8161 tacacggtag acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga
     8221 caagattacc attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta
     8281 attatgaata acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc
     8341 catgctcttt cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa
     8401 tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt
     8461 tccgcctcta ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc
     8521 atcggctgca atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat
     8581 gtggcgtact cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta
     8641 cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac
     8701 cgtggcaagg tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa
     8761 gcggttattg agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga
     8821 agtatagagg taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg
     8881 cttcatgtgc cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga
     8941 atgaagcaag gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag
     9001 ttggttaaag cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga
     9061 gaggaagagt ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt
     9121 aaacttcaaa gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa
     9181 gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag
     9241 gagcatgaat agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt
     9301 atcggtaaaa tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt
     9361 atacattgga attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg
     9421 ggaaaacgac aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact
     9481 tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca
     9541 tggcaagtca ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt
     9601 tgtaggctgc gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt
     9661 tgcgaaaaat gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc
     9721 ggtggcagct acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt
     9781 cggtgtgaaa aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca
     9841 atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt
     9901 attgggaaac agtgccgcgt tagttgttgg cgaggtggac caaatcaggc tgcagtacgg
     9961 aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac
    10021 cgttcccgca gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat
    10081 atataaagcg ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg
    10141 ccgcattgta ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc
    10201 ccgtatgatg gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt
    10261 agcgttaaag gggtgataag catggaaata ggatttactt ttttagatga aatagtacac
    10321 ggtgttcgtt gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac
    10381 ggttatgaag taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca
    10441 aaagaactgg ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaag
    10501 cgtgctgtaa actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa
    10561 agttattatc ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa
    10621 tcaagccata gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag
    10681 cttgtaccaa tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat
    10741 ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt
    10801 gggtttgaag catatagcct cgaatggtgg cactatgtat taagagacga accatacccc
    10861 aatagctatt ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat
    10921 aagctaactc tttcggcagg aaacccgacg tatgtaactg gttcttaggg aatttatata
    10981 tagtagatag tattgaagat gtaaggcaga gcgatattgc ggtcattatc tgcgtgcgct
    11041 gcggcaagat agcctgataa taagactgat cgcatagagg ggtggtattt cacaccgccc
    11101 attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg gtatcactta tgaaaattca
    11161 tctacattgg tgataatagt aaatccagta gggcgaaata attgactgta atttacgggg
    11221 caaaacggca caatctcaaa cgagattgtg ccgtttaagg ggaagattct agaaatattt
    11281 catacttcca actatatagt taaggaggag actgaaaatg aagaagttgt tttttttatt
    11341 gttattgtta ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca
    11401 ggaaaaagtc gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg
    11461 gacttctgaa aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct
    11521 gctattaatc aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa
    11581 tttatctaaa catgacgaat taataaatgg atacgggttg cttgatagta atatttatat
    11641 gtcaaaagaa atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt
    11701 tagtcatttt attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca
    11761 agaaatgggg gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc
    11821 actagatgta ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga
    11881 agaaaatgct tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac
    11941 aggaattcaa tatgaaacat ggcatattcg ctatgttggt ttaccacata gtgcgattat
    12001 gaaagaaaag aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat
    12061 ttctgttagt gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac
    12121 caccattcat gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt
    12181 aattgtgaca gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa
    12241 tgaaaccaac gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc
    12301 tttttaattt aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca
    12361 aaagaattag aggaaattat attgggaaaa atattatcta gaggattgct agctttatat
    12421 ttagtgacac taatctggtt agtgttattc aaattacaat acaatatttt atcagtattt
    12481 aattatcatc aaagaagtct taacttgact ccatttactg ctactgggaa tttcagagag
    12541 atgatagata atgttataat ctttattcca tttggcttgc ttttgaatgt caattttaaa
    12601 gaaatcggat ttttacctaa gtttgctttt gtactggttt taagtcttac ttttgaaata
    12661 attcaattta tcttcgctat tggagcgaca gacataacag atgtaattac aaatactgtt
    12721 ggaggctttc ttggactgaa attatatggt ttaagcaata agcatatgaa tcaaaaaaaa
    12781 ttagacagag ttattatttt tgtaggtata cttttgctcg tattattgct cgtttaccgt
    12841 acccatttaa gaataaatta cgtgtaagat gtctaaatca agcaatctga tctttcatac
    12901 acataaagat attgaatgaa ttggattaga tggaaaacgg gatgtgggga aactcgcccg
    12961 taggtgtgaa gtgaggggaa aaccggtgat aaagtaaaaa gcttacctaa cactatagta
    13021 acaaagaaag cccaattatc aattttagtg ctgaggaatt ggtctcttta ataaatttcc
    13081 ttaacgttgt aaatccgcat tttcctgacg gtacccctat catagcattt ttaaaaataa
    13141 attttatgta gacttaaaat ttacttcatg gagggtttaa atggatgaaa tcaaaagtaa
    13201 caaaaaagca tttggcattt atttaggaga acctagtaca gaacaattaa accaattttt
    13261 ttatttgcat gaaaaagatt tagaattgat ggagactata cgacgaccag ctactagatt
    13321 aggttttgca ctacagttag tctcactacg ttttttagga acttttatat tagatcttac
    13381 caatattccc caaaatgtat tgagttatgt agcaactcaa ctaaatattg atcctggaga
    13441 aattatctac tatagcagga gacagaccag gtatcagcac acaaaattga taaaatcaca
    13501 ttatggatat caagatttca accaaccgga aatagaaaca tacctagcta actggctttt
    13561 aaatagagcg ctgtatacaa cagaatcttc agatatgttg tttgatatgc ttctaaaaaa
    13621 atgtctggat gaaaagattg ttttacctgg aattactact ttttctcgtt ttgttgctag
    13681 tgcaattgaa aaagctgaag agcatttata ccgacaattg tctttggttc caactgaaga
    13741 tgagagaaag cggcttctca atcatttaga attaattgga actccagttt atggggcaat
    13801 aatcaaaatg gacattttga gaaccccgtt agctgacgat agtcataaag aaatagccag
    13861 aggttttgac cgactaaagc aatttcaagc tttctctact gaaaaatgga agatggaatc
    13921 tattcctgaa gggaaaataa aagttcttgc aaattatgca tttaaagcta aagctcaatt
    13981 aattcaacga atgagtgaac agaaaaaact ggcgttgtta gtggcattta ttttcatata
    14041 caaacgtaag gctatggatg aacaaatttt agcactatct aatttttttg aaactgtttt
    14101 tagacgcgcc aaaaataagg aatctaaaga acgtcttcgt accataaaag atctagatcg
    14161 agccgcatcg acactttcag atattgtaga tttagtaatg tccagtcatt ctgatactaa
    14221 tgaactatcc cttcgcgatc gtatcttaga aaaatattct gaggaaaatg ttaccgaagc
    14281 tgtatcgcaa gttcggctat tggttaaaaa tgatcgtgaa ccaatagcca tttctgaact
    14341 tctgaacact tatcgaaagt tcagaaaatt tattccggat attttaacta cactaacatt
    14401 tgacagcaat gactacggta acaactgtaa ggaactctgg accttcatta ataatagatt
    14461 tcctaagccg attacttaca aaatgtttgg agctattaaa catactcttc caaagaaatg
    14521 gatttattac attcatgaat accctgaaca aactaataaa tgtgtattga ttgcagctgt
    14581 cgaattgtta attcaatctt taaaacgcca tgatatttat attcctcaga gtgtcttata
    14641 taacaatcct atggattgct tgattgatga gaaaacttgg gttcaacaaa aatacgtttt
    14701 attagaacaa ctagatcttc cttcttcagc aaatgaaatg tctttatggt taaagaacga
    14761 tttggaactg tcttataaag aagcgctaaa tcgttggcct acttctaaaa tggccagaat
    14821 agaaaatgca gatcgcctgg ttgtctctag acttaaaaag acatatgaaa aaaaggaaga
    14881 taagtctttt agacgtcgca ttcagcagtt acttcctaaa attgatttgt cagatttgtt
    14941 attggaagta aaccaacagt taaatcttac tcaatcattt aaccacttaa gtgacaagga
    15001 caccaaaatg aaagacttgg atattagttt attagcagtt cttttatcag agtcctgcaa
    15061 tattggtttt tccccggttt caaaagaagg atttgattct ttaaagtatg atcgtcttgt
    15121 ctatgtcaat catcattata ttcgagtgga tacactttca ggagctaatc aaaaaataat
    15181 acatgcacac aggaaacttg ccacctctaa gatatggggt gatgggcata tggcttcagc
    15241 tgacggtatt cgttatatca caccacaacg ttccctatat tctagaagta atccaaaata
    15301 ttttggccgt ggtcgtggga tcacattcta caattttgtc tctgatcaat atatagggtt
    15361 ccacggaatg gtcgtttcag ggacactgcg agattcgctt taccttctag aaggattcct
    15421 caatcaaccc agcgaattaa ctcctaatca gatcatgact gatacagctg gttatagcga
    15481 tatgattttt ggtctctttg gcttattagg gtttcaattt agtccaagaa ttgccaatga
    15541 taaaggaacc aaactttggc gaatcgacgc taacgctcac tacggggaac ttgatacgtt
    15601 gtctcaaaat tcaattaata tggaactgat ttatacccat tgggaggaca ttcttagggt
    15661 tgcaggttcg ttgaaatctg gaaaagtgaa tgcaacagaa ttaacgaaat ctcttcaacg
    15721 gaatgggcaa cccacgcctt taggaaaagc aataactgag tatggaaaag tttataagac
    15781 gaaacatcag cttcgttata tttctgacga aatatatgca cggcaaattt tagaacaatt
    15841 gaataagggg gaagctcggc atgccttatg ccgtaatatt ttttatggac gaaaaggaaa
    15901 actctaccag acttattatg atgggatgga agagcagctc aatgcattag gattagttac
    15961 taatgccatc atatattgga actcactgta tttagaaaaa gtatttgaac aaatgaaagc
    16021 tgaaggattc gactgttccg aagaaatgat acgcaggcaa tctccactaa ttagtgatca
    16081 catcaatttt gttggtaagt atacgtttag atacgaagct gaattatcca acggtaagtt
    16141 acgtccattg aatgtagaag aattctagca atgttcgggt tttgcatgct accgcactat
    16201 ttcgtatgac tgttccagcg aatcacttcg aatagaaaaa gagaaaaaca cgagaacagc
    16261 gttaaatcaa cgtttcttgt gtttttactt ttgtgctacc gcactatttc gtttctatgt
    16321 tccatgtacc ccatatttta ataactattg tgtttatcaa ccaaatggtt tataatattc
    16381 ttagcgtaaa cttggaggtg ctgtatgact acggcagaaa tgattaaaga actgtgtgag
    16441 caaatgaata taagtgtttc cgaacttgct agacgtattg gccagactcc acagaatttc
    16501 aataaaaaat tacaacgaga aacagtaacc ttggatgagt tgaaggccat tgctgatgtg
    16561 ctaggtgtca agtttgtgca ggcatttatt ttaccagatg gagaagaaat aaaaatatct
    16621 aacgaataaa ggaggatgtt tatatggtag gtaatgttat tcaaatagta acagagaaat
    16681 tatcatcttt gccttttata gaaggcatcg tattaggggg ctcacgtgca agaggcaccc
    16741 atacagaaaa ttctgatata gatatcggaa tctattacaa ttcagactca tttgacctga
    16801 cagcgattaa tcaaattgct acagaattgg atgataaaaa tagaaacaac cttgttgtgc
    16861 ctcccggagc gtggggtgat tggattaatg gcggcggatg gttatttata aatgggtatc
    16921 atgttgactt gattttacgt gatataaaac gggtagaaca aataatcaaa gatacggagc
    16981 aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata agtgcaatgt
    17041 atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc ttatgcgaat
    17101 taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg aactttttta
    17161 tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggta gaggataaat
    17221 attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta ctatttgcat
    17281 gtaataatgc ttattgcatt aacgaaaaga aagctataaa actgcttgaa acttttgaat
    17341 ataaacctga aaaatatgcc gaaagagtta atcatatttt tgaagtactc ggtctttctc
    17401 tttttgaatg ctacgatatg accgagaaac tttataaaga agtgaatgaa attgtatcgg
    17461 agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat
    17521 atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga
    17581 gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta
    17641 ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta
    17701 ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag
    17761 attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta
    17821 atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata
    17881 tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact
    17941 gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg
    18001 gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa
    18061 tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt tataataaat
    18121 cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa
    18181 atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa
    18241 aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt
    18301 tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta
    18361 atatacctaa agatgaattt caggattatg atattacata ttttgtaagt gatatagaac
    18421 cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc
    18481 cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg
    18541 atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa
    18601 agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa agggacatag
    18661 ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct
    18721 gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgc aaagagatac
    18781 tgtttgcaat cgatcatctg aaccagattc tacggtttga actacttagg atgatgtcgt
    18841 ggaaggttgg gataaagaca gaattttcat taagtgttgg gaaaaattat aagtatatta
    18901 acaaatacat tgatgaagat ctatggaata gattattatc tacatatcgc atggattcct
    18961 atgaaaatat ttggaagtca ttatttatat gccaccaatt gttcagggaa gtgtccaaag
    19021 aggtagcaga actactgggg tttgattatc cagagtatgg taagaacata acaagatata
    19081 ccgaggacat gtataaaaaa tatgttgaaa atgactattt ttaaagatta gcaaagatta
    19141 ttaaccataa gcaaggggtg taaatgtatg gaaagtactg atgttataaa tgaaattaga
    19201 ggtatattac ctcgtaactc aattggaaaa tatgatttac taactatttt tacacataaa
    19261 accgtttttg ataaaattgt aaaagtacta tcattggatt ttcaaaataa agtagattat
    19321 gtggctgccc cagaagcaat tggatggata ctaggcactg ccatagccaa agaacttgga
    19381 gtggggttta ttggagtaag aaaaggtgat aagctgccat acgcaaaaga agagattatt
    19441 tcgacacatt ttaccgacta ttcaggtcaa gataaaagct ttgaaatatc taagagttct
    19501 attgtgagga acaaaagagt tttgattgta gatgattgga tagagacagg ttctcaaatg
    19561 aaggctttaa tagctttgtt agagaaattg gattgcagca ttattgggtt ggctactatt
    19621 gggattgata taaatgaggt tactcaaaaa tggatagaca gcagttttgt agcttatatt
    19681 ggctcaaaca tataataaac cgaaagaaaa tcttgaatca aaattttgga tggcgttaat
    19741 ttacaataca acgaaagatg tttaccctga ataattcata ctttttaatt gaaaccgtct
    19801 gaaactgcct aacggcagtc tgataattac ttttttagtt tcacccctta agtgatgaaa
    19861 aaagaacccc attctgctat ggtaaaggtg acgaaaccaa ccataagaaa ggagttcttc
    19921 tcatgactag cttacacaaa aatcaagtta aattcaattc aaatatcact atttcacata
    19981 ccggtggtcg tctatcaagt gattcggggt tagtcctggt taaagaatta atgaatacct
    20041 tcggcttttc gcacttagct aaacaatggc tccatattac agatcatcgg gcttatttta
    20101 cccatgataa cttagcgata ttggaacagc ttattatgca attaattgct ggttactcgg
    20161 cagactcatc agctaatcta ttaagacggg atcctgtctt tcaagctgta ctcggtaaaa
    20221 aacagttggc atcacaatcc tcaatctcac ggtttctgga tcgtttcacc gaggagaaca
    20281 tcgatcaact ccaagcatta aatcagtcat tgattgataa agcacgttta attcgcaatg
    20341 acaccgagtt aatcattgat gtcgattcca cacattcgga tacctttgga cgccaagaac
    20401 aaacagatta taatgcccat tatcaaacct acggctatca cccattagtt gcctttgatg
    20461 gactgaccgg agatttctta aaagctgaac tgcgttcagg caatcagtac acatctaaag
    20521 gggtaaaagc ctttatcaac ccgcttttac accactacaa gagcacgtta cctcataccg
    20581 agatattggt tcggggtgac agcggcttcg ccacaccaga ggtgtatgag tcttgtgaag
    20641 caactgaaag ccagtacgtt atccgattaa agagcaatcg gaggttaagt cagttagctg
    20701 aacattctgt tctttacggg gacaatcaag aatgggaaga gcgagaagtg cagtattttt
    20761 cactacctta tcaagcacaa tcctggtcga aacctcgtcg tgtctgtata cgctccgtcc
    20821 gtgaaggagg agaattgcta tttcatcatg cgtttattgt gactaatcta tccgacaatg
    20881 tctcgcctga agtcatattt tctctttacg gcaagcgagg aacaatggaa aatttcatca
    20941 aagaagcgaa aggtggcttt tacttcgata aaacagatag cccgcgtttc ttagaaaatc
    21001 atgtcagaat gatgatcagt gtccttgcat acaacctcgt taatttttta aaaagcattg
    21061 gttttgataa agtgaaccag ggcatgacga ttcattccat ccggctgata ttgcttaaag
    21121 tagccggtaa acttgttcag actgggagac aggtctatct caaactgtca agttatcatg
    21181 tgtatcaaac tgaattttat aaggtttttg aacgcctgcg gcgatccaaa caatggattt
    21241 agactagtta tcacacaaat tttttaaccg attctttttc aagggattcg tctgccctta
    21301 aatagacaaa taatttttat tatgatcttc ttccgtaggc atcctgttcg aaaacacatt
    21361 attttggaag aaagtatctg cctaatttaa aaattggcaa tttgttcaaa aaaacaggtt
    21421 aaatttagag ctatgaatta ttcaggtttt ttcgaggtgg aagctagtat aaaagtaaac
    21481 caagggtcgt ttaagggcgt tctaagcccc acacagcgtt ttaagagcca agaggaactt
    21541 ttatcctttg tttcttctaa acaagctaaa ataggcaatc aagaggggat aattgctgat
    21601 gttcaaacca aagagaagaa aacgaatagt ccgagtttgt tttctttaag tagtttacaa
    21661 tcaaaagtca atcagcttta taaagcgaca gcgagccaaa ctttaaaagc tatgcaagga
    21721 ctgtatgaag caaaattatt gagttatcca agaacagata caccatttat tacagagaac
    21781 gaatttgctt atttaaaagc aaattttggc aaatatagcg gttttttagg acttgatctc
    21841 gaaatggttc aaacagagcc tagaaagcgt tatgtggacg gtagtaaggt acaggaacac
    21901 cacgccatta tcccaacaaa acaagtacct accgaatctg cattagcaaa aatggacgat
    21961 ttacaacgaa aaatatatgc tttagtcgtt aaaacgaccg ttgccatgtt tttacctgat
    22021 tacttgtatg aagaaaccaa gatacaaacg aaagtagctg atttgctgtt tcaatcgatc
    22081 ggaaagacac caaaacaaga aggttggaaa attcttttca aacaacaaac caaagaagaa
    22141 aaagaggacg ttcaaacgtt acccttggtt atcattggcg aacatgccga ggttgacgtt
    22201 aagagtgccg aaaaagaaac acaaccaccg aaagctttta cagagggtac attattaact
    22261 gctatgaaaa ccgctaataa aacggttgat gatgaagaag caatcaagat tttacaagaa
    22321 gtcgagggga tcggaacaga agcgacaaga gcaagtatta ttgaagcgtt aaaacaaaaa
    22381 gaatatatcc aagtgattaa gaataagctt gttgtaactg aaaaaggaaa attattgtgc
    22441 caggcagttg aaagtcagca ccttttaacg agtgctgaaa tgacggctaa atgggaaacg
    22501 tatttaaaaa aaatcggtaa aagagaaggc aatcaagaaa actttattaa taatatcaag
    22561 aagtttatcg ttcatttact ggaagctgta cctaacgata tagaaaaact aaatttttct
    22621 gattaccagg aacagaaaga aaaagaagct gaaaaaagta ttgtaggaaa atgtcctaag
    22681 tgtggcaaca atattgtatt aaaaaaatcg ttttatggtt gttcaaatta tcctgaatgt
    22741 aagtttactt tagctgaaca ttttagaaag aaaaaactaa ccaaaacgaa tgtaaaagaa
    22801 ttactggagg gaaaagaaac cctggtaaaa ggaatcaaaa acaaagagaa aaagccctac
    22861 aatgccgttg taaaaatcgg agaaaaggga tatattgatt ttatctcttt ctcaaaataa
    22921 acataaaagc cctttaaaca gggcttttat atattaatca caaatcacct atcacaaatc
    22981 acaaatcact tgcgatttgt gatgataaaa taagagtaag aagaaataga aagaagtgag
    23041 tgattgtggg aaatttaggc gcacaaaaag caaaacgaaa tgatacacca atcagtgcaa
    23101 aaaaagatat gatgggagat aagacggttc gtgttcgtgc tgacttgcac catatcataa
    23161 aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt tatggaaata agacttagaa
    23221 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
    23281 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
    23341 aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
    23401 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
    23461 agataactaa aattacaaac aaatcgttta acttctgtat ttatttatag atgtaatcac
    23521 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
    23581 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
    23641 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
    23701 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt
    23761 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
    23821 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
    23881 ggataaagtc cgtataattg tgtaaaagta aaaag
//
LOCUS       DAZKCN010000270        12793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.073, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000270 DAZKCN010000000
VERSION     DAZKCN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.073"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8K3_002822"
     CDS             complement(134..577)
                     /locus_tag="ACG8K3_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP9146592.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8K3_002823"
     CDS             complement(795..2135)
                     /locus_tag="ACG8K3_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9146593.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8K3_002824"
     CDS             2404..3300
                     /locus_tag="ACG8K3_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP9146594.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8K3_002825"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8K3_002825"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9146595.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4516..5637
                     /locus_tag="ACG8K3_002826"
     CDS             4516..5637
                     /locus_tag="ACG8K3_002826"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP9146596.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5895..7397
                     /gene="eat(A)"
                     /locus_tag="ACG8K3_002827"
     CDS             5895..7397
                     /gene="eat(A)"
                     /locus_tag="ACG8K3_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP9146597.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7464..7991
                     /locus_tag="ACG8K3_002828"
     CDS             7464..7991
                     /locus_tag="ACG8K3_002828"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP9146598.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8202..9263
                     /locus_tag="ACG8K3_002829"
     CDS             8202..9263
                     /locus_tag="ACG8K3_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP9146599.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9266..10162
                     /gene="murQ"
                     /locus_tag="ACG8K3_002830"
     CDS             9266..10162
                     /gene="murQ"
                     /locus_tag="ACG8K3_002830"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9146600.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10176..12158
                     /locus_tag="ACG8K3_002831"
     CDS             10176..12158
                     /locus_tag="ACG8K3_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP9146601.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12223..12669)
                     /gene="nrdI"
                     /locus_tag="ACG8K3_002832"
     CDS             complement(12223..12669)
                     /gene="nrdI"
                     /locus_tag="ACG8K3_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9146602.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         4200 a         2338 c         2659 g         3596 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggtgttccag
       61 aagtagcttc ttcttatttc ttgaagctga tcacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg gatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgt caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gttccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaataacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat ggctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgattagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccgccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa gcgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttaat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat ggatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttgcttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ctatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc ttttaagcca
     3181 atcccatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaataaataa
     3301 aaaacctaca ttttgaagag attatgtacc gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttc attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc taatatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gttttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cattatggct atagatcgga tttaattttg cttcttgaaa gacttccaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacgaaa catatcatct
     4141 aattgattcg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtgt gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc tccagctatt
     4381 gcgattttca tgttttttct ctcctttatc aatgagttcc cttttagtat atagaaaaat
     4441 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     4501 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     4561 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     4621 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcggg
     4681 ggaacatggc gcaccattga tgggatggat cttttattcc gtcagcgaaa aatcagctat
     4741 ttattgacaa cttggcaagg aaaaaatacc gcagtcatcg aatctgcaca agcattgggg
     4801 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
     4861 ggagaactta tcaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     4921 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     4981 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     5041 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     5101 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
     5161 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     5221 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     5281 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     5341 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     5401 ggaaaagttc cacacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     5461 atttgcggaa gtagagacca agagctagaa ggactctctg attttctccc aattgtctta
     5521 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     5581 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     5641 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     5701 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     5761 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     5821 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     5881 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     5941 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     6001 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     6061 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     6121 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     6181 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     6241 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     6301 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     6361 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     6421 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     6481 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     6541 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     6601 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     6661 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     6721 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     6781 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     6841 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     6901 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     6961 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatcc cagccaaaac
     7021 atctgagcat tagttatgta cgccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     7081 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     7141 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     7201 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     7261 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     7321 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     7381 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     7441 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     7501 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     7561 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     7621 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     7681 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     7741 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     7801 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     7861 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     7921 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     7981 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     8041 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     8101 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     8161 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     8221 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     8281 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     8341 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     8401 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     8461 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     8521 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     8581 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     8641 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     8701 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     8761 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     8821 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     8881 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     8941 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     9001 atgtcatacg aagcgcagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     9061 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     9121 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     9181 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     9241 ttataagaaa agaaggaaga taaaattgag caaaatcaat ttagagaacc taacaacgga
     9301 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     9361 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     9421 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     9481 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     9541 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     9601 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     9661 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     9721 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     9781 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     9841 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     9901 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     9961 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
    10021 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
    10081 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
    10141 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
    10201 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
    10261 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
    10321 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
    10381 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
    10441 taggagaaaa actttcttct gtaagtgcag ctacagcaaa ttcatcagca tctggaaaag
    10501 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
    10561 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
    10621 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
    10681 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
    10741 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
    10801 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
    10861 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
    10921 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
    10981 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
    11041 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
    11101 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
    11161 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
    11221 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
    11281 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
    11341 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
    11401 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
    11461 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
    11521 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
    11581 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
    11641 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
    11701 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
    11761 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
    11821 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
    11881 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
    11941 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
    12001 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
    12061 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
    12121 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
    12181 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
    12241 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
    12301 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
    12361 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
    12421 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
    12481 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
    12541 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc
    12601 agcatattct gctagatgtt tggcaaaggc acgcgtattt cctgaaatcg atatatataa
    12661 aatgttcatg gatcatcctc caattctttc cttttagtat agcatctttt ttaagttttt
    12721 tggtggtgta atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagccggt
    12781 agaattatat ttc
//
LOCUS       DAZKCN010000271         9958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.094, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000271 DAZKCN010000000
VERSION     DAZKCN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.094"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..991)
                     /locus_tag="ACG8K3_002833"
     CDS             complement(119..991)
                     /locus_tag="ACG8K3_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9146603.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1119..1688
                     /locus_tag="ACG8K3_002834"
     CDS             1119..1688
                     /locus_tag="ACG8K3_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP9146604.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1755..2492
                     /locus_tag="ACG8K3_002835"
     CDS             1755..2492
                     /locus_tag="ACG8K3_002835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9146605.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2723..3991
                     /locus_tag="ACG8K3_002836"
     CDS             2723..3991
                     /locus_tag="ACG8K3_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP9146606.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4478..4942
                     /locus_tag="ACG8K3_002837"
     CDS             4478..4942
                     /locus_tag="ACG8K3_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9146607.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4926..5555
                     /locus_tag="ACG8K3_002838"
     CDS             4926..5555
                     /locus_tag="ACG8K3_002838"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP9146608.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            5909..7387
                     /gene="msr(C)"
                     /locus_tag="ACG8K3_002839"
     CDS             5909..7387
                     /gene="msr(C)"
                     /locus_tag="ACG8K3_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP9146609.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7434..7880
                     /locus_tag="ACG8K3_002840"
     CDS             7434..7880
                     /locus_tag="ACG8K3_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP9146610.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8079..8594)
                     /locus_tag="ACG8K3_002841"
     CDS             complement(8079..8594)
                     /locus_tag="ACG8K3_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP9146611.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8711..9912
                     /locus_tag="ACG8K3_002842"
                     /pseudo
     CDS             8711..9912
                     /locus_tag="ACG8K3_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         3172 a         1747 c         2074 g         2965 t
ORIGIN      
        1 ggaattatca gaaataaaag gaagaaataa attaagaaac ttatgttatt taatcgttgg
       61 atcttttgct tatcttattc gttgttaaaa agctgaaagc gaaggaaaaa aactctactt
      121 aactgtattt ctccgttagg acatctaaaa atttacgagc agcaggagaa agaggctggt
      181 cttttttcca aatgagattc agtgaagctt gtaatggcgg atcgagtggg atgaatattt
      241 tttccgtccc ataagtattg atgatcccat ctagagctaa tgcaccaaaa gtcccgttat
      301 cgaccatcaa agaagcattg tacaataagt tatatgttcc gacgatcgat aactgatcta
      361 tgctgaaacc tagccattca gctagttgac tgtccactga cgtttgccgg gaaatgatca
      421 aaggaacagt tttcagttcc tgcggatgga tgacggtttg ctttgctaaa ggatgatttc
      481 gatgtatcaa aagtccccaa cgatcttggg ctggcagtcg aaatgactgg tatttttgtt
      541 tgtttgtcgg ttcgattacg agtccaaaat caagtaatcc gctatccagt ttgtctatga
      601 tatcatctgc attcccgcta tataaatgaa actggatatc aggatgcgcc ttgatcaacg
      661 aatgaaggct atcagaaatg aaagagattg ctttagtttc gcctccgcca atgtagattt
      721 ctccagagat gattgcgtca tttgttaaat tgttcgccgt tttttctacc aacgacaaga
      781 tttcttttcc ttttgaataa agatatcgac cagaatccgt caactgtatc tctcgggagc
      841 cccgagtgaa aagtgtcaca ccaagttctt cttctaaatc tttcagttgt tttgagagag
      901 taggttgcgt gatatgcaga gattttgctg ctcggctgat atttttttct ctagctactg
      961 ctaaaaaata cgtcagtata cgaatatcca ttttttccct cctgttttta aaaaagcata
     1021 ccactaagta tagttaaaag gaatagttaa gtatgtttta taagtattag acatgataag
     1081 ccgtccgtta taaagtaagg ataaaaggag ggagcaaaat gacaagcctc atcgaaaaac
     1141 aaatcgtcaa taaagagatt cgagtaacgg atccgctctt tgaagtgatc catcagatcc
     1201 aagctgaaaa cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa ccagcagata
     1261 tccgcaaacg gctagaaaat atcattagtg acaaagtaga tgatacggtg actgttctgc
     1321 tgccttttta tacagatttc ggcaaacata ttagtatagg gaaaaatgtc tttatcaatc
     1381 gtcaagtgat gttcgttgac ttaggtggca tctgtttaga agattcagta ttgattggtc
     1441 ctcgtgtcaa cctcatcacg gtgaaccatc taaccgatcc aaaagaacga agaggcttgt
     1501 cagtaaagcc aatccacatc aagaaaaatg cttggatcgg agcaggagca actattctgc
     1561 caggcgtaac gattggcgag aatgccattg ttgcagcgaa tgcaactgtt acgaaagatg
     1621 tcccagacaa taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct gtcgaacgga
     1681 ttatttgaac agtaagatga aataagagat agtgagtata agcaagagat ttttcaaaac
     1741 aggaggagaa tagaatgaat gatttatcag aaaaagtgat tgtcatcatg ggcgcttcaa
     1801 gcggaatcgg tgaagcaaca acgaagctat tagcagaaaa aggggctaaa ctagttatag
     1861 ctgcacgccg agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa gctgagctat
     1921 atattcaaac agcagatgta agagattttg cacaagtcca agcagttatc gatttagcga
     1981 tggaaaagtt cggtcgaatc gatgtattgt acaataatgc aggaatcatg ccaaccgctc
     2041 cattagttga aggtcaccgt gatgaatggc aaaatatgct tgatatcaac atcatgggtg
     2101 tgttaaatgg aatttccgcc gtacttccaa tcatggaaaa acaaaaatct gggcatatca
     2161 tttcaactga ttctgtggcg ggacatgttg tttatcctga ttccgccgtt tattgcggaa
     2221 caaaatttgc tgtccgtgca atcatggaag gattacgtca agagcaacgg caaaataaca
     2281 tcaaatctac gatcatctct ccaggagctg ttcaaactga attatatcaa accatcagcg
     2341 acaaaaaggc tgctcttgaa cttcatgaag cgcaaaaaga atggggattg acctccgaag
     2401 atatagcttc tgctgtagca tttgcgatag aaacgccaga ccggatgtct gtcagtaaca
     2461 tgattatccg tccgacgaca caggaagtat aatccataac ggataatatc caaagagaat
     2521 tacttgttaa atagttattc aaacgaccat caatttaagg cgtgaatgga gaagaatatt
     2581 cttcatttac gtctttttat gtagaaaaag caaatttatc aaaaagaaac aaatgttatt
     2641 taaatatgga cattgacttg cttttacttt tttagttaca ctgttattta ggaaaagttt
     2701 cataattcaa aggagaaaaa agatgaaagc aaagaattta ttatttagcg ttattctttt
     2761 aggaaatcta gcagttgcca acacaacagt gctagctgaa gaatcgacag aaactgtcgc
     2821 aactcagaca tcgagttctt cagaaacagg aattgaaaac gatacagaga gtactttgca
     2881 gtcagctact gtctcagaaa atacaatttc aaccgaagaa ccaacaactg aaacaacaga
     2941 acaaacacaa accgaagaca gtacatcagc agaagctcag aacaatccag agaatgcaga
     3001 agaaatcacg aaccaattga cagatgggca aacattgaca catacccatc aagcagattt
     3061 gtctgaattc gaaaactata ctggctatgc tttttatgct cgctcatctg ctaattctca
     3121 acaagcgttc attgatagta ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt
     3181 atatgcatca gtcatgattg ctcaagccat cgttgaaagt ggctggggga acagtacttt
     3241 ggcttctgcc cctaattaca atctatttgg gattaaagga agctataatg gccaatctgt
     3301 tacgatgcct acttcggaat atgtgaatgg gcaatggatt acggtgaatg ccgcttttag
     3361 aaaatatcct tcgtacaaag aatcattgca agataacgta acggtattga agacgacttc
     3421 ttttcagcca ggcgtttact actattccgg tgcatggaaa agcaatacga actcttacaa
     3481 agatgctacg gcttggctaa caggtcgtta tgccactgcg cctaattatg gttctacatt
     3541 gaacaatgta atcgaaacct ataatctgac acagtatgat acagcaccaa cttcaacttc
     3601 tccgatgtat cgtctgtata accgtcatac aggagaacat ttatatacat tgaatgccgg
     3661 agaaaaaaat tatctgccaa ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc
     3721 aaacagcgga caacctgtct accgtttata caatccttac agtggagacc atcattacac
     3781 aatggcacaa tctgagatca actctttaac aaaaatcggt tggcgttatg aaggtctaag
     3841 tttttattct ggtggttcca agccaattta tcgactattt aatccaaatg aaaaaactgg
     3901 tacccatcac tataccttga gtgctaagga aagagattgg ctaacaccga tgggctggag
     3961 atatgaagga ataggatttt atggttatta aaaagaggta gtgaaaaagc tgccctgcat
     4021 caagtagtaa gaacagccaa acaaaaacag attttcatgt tttttgtttg gttgaactta
     4081 ttaccgcaga tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga caacatcttg
     4141 tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt
     4201 cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttttctactg cagaagagct
     4261 atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca
     4321 ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt
     4381 tttttattac acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc
     4441 aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca
     4501 ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat
     4561 cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct
     4621 acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa
     4681 ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt
     4741 gtatgtagcg ctaacagaaa aagcccagaa gaaaatcgag cattgtgaaa tggaccataa
     4801 agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac
     4861 acttttagat aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa
     4921 taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg
     4981 tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga
     5041 cgactgcacc ttcttctgtc aatatgcaac catggcgctt tgtggtcgct gaaagcgagg
     5101 aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt
     5161 ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctata
     5221 accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga
     5281 tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt
     5341 ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc
     5401 caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt
     5461 atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc
     5521 gtttagcacc agaaaagatc actacgttcg aataaaaggt tttcagttac tattcagtag
     5581 ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac
     5641 tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat
     5701 gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg
     5761 tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga
     5821 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
     5881 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
     5941 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
     6001 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
     6061 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
     6121 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
     6181 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
     6241 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
     6301 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
     6361 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
     6421 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
     6481 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
     6541 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
     6601 taaaaagaaa aagcaacaaa tcagaccgga tcggttgtct tcctctaaac aaaaagacag
     6661 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
     6721 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
     6781 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     6841 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     6901 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     6961 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     7021 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     7081 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     7141 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     7201 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     7261 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     7321 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     7381 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     7441 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     7501 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     7561 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     7621 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     7681 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     7741 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     7801 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     7861 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     7921 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     7981 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     8041 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     8101 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     8161 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     8221 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     8281 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     8341 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     8401 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     8461 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     8521 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     8581 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     8641 gtttccacgt tttacatgtg catatttaca tgtaaaataa atcatgtatg atgctaatag
     8701 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     8761 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     8821 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     8881 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     8941 aggtttctta tctagtcgga tcggtttgaa aaaacattga tgacaggatt gtttatcatc
     9001 gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt
     9061 gccggctggg gtgtcgggaa tgctttattt gtctctactg ctttagcaac gatcgtcagt
     9121 attttagttg gtaacacaga aaaagcaatc atgatgtatg aagcagcact tggctgcgga
     9181 atggctgttg gccctctagt cggtggggtc ttgggcagtg gttcatggcg ttatccattt
     9241 tttggggtag catttttgat gttttgcggt tttatagccc tagcggtact tttacctgaa
     9301 actgagaaac caaagagaaa agtaggcctt tgggaaggtg tcaaagcgtt aagcaatcat
     9361 aaacttcgtt cagttggact aatcgcattg ttatataatt tcggattctt tactttactt
     9421 gcgtatgcgc catttttatt gattggctat tctgaattag aagtcggttt tgtatttttt
     9481 ggctggggtg tgcttctggc tattgcttcg attttcgttg caccggctct tgagcggaag
     9541 tttacgactt atcggacaat tttagcagca tttgtcttat tcattatttg tttgctgtta
     9601 ttaggagctg gaacttctat gccagtcctt gtaccgatca gcattgtgtt agcaggattt
     9661 ttccaaggaa ttatcaatac actgttgacg acgattgcta tggaaatacc aggtctgcaa
     9721 cgaaacgttg cttcgtccag ctatagtttt gtccgctttt tcggtggagc tttagcacca
     9781 tttattgcag gtgtgattgg tgaacatatt ggtgaaagct atgcgtttta ttttgcaggt
     9841 gccattgttc ttatcagtgt atgttgtatc atttatcacc gtaattattt tgctacaaat
     9901 gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DAZKCN010000272         6642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000272 DAZKCN010000000
VERSION     DAZKCN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.123"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..209
                     /locus_tag="ACG8K3_002843"
                     /pseudo
     CDS             1..209
                     /locus_tag="ACG8K3_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            447..683
                     /locus_tag="ACG8K3_002844"
     CDS             447..683
                     /locus_tag="ACG8K3_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146612.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="ACG8K3_002845"
     CDS             690..2642
                     /locus_tag="ACG8K3_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9146613.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8K3_002846"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8K3_002846"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9146614.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3486..4613
                     /locus_tag="ACG8K3_002847"
                     /pseudo
     CDS             3486..4613
                     /locus_tag="ACG8K3_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
     gene            4711..6084
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002848"
     CDS             4711..6084
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFP9146615.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         2196 a         1055 c         1244 g         2147 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg
     4261 tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg
     4381 cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg
     4441 ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt
     4501 gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc
     4561 gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt
     4621 tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat
     4681 ttgaattaaa gtgtaaagga ggaattaatt atgaagtgta atgaatgtaa cagggttaaa
     4741 tacaaccaaa ttttaatttg gctttgcgtt ttatcttttt ttagcgtatt aaatgaaatg
     4801 gttttgaacg tctcattacc tgatattgca aatgatttta ataaaccacc tgcgagtaca
     4861 aactgggtga acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag
     4921 ctatctgatc aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc
     4981 gggtcggtaa ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt
     5041 attcaagggg ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat
     5101 attccaaagg aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga
     5161 gaaggagtcg gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt
     5221 ctactcattc ctatgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa
     5281 gaagtaagga taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt
     5341 gtatttttta tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg
     5401 tcattcctga tatttgtaaa acatatcagg aaagtaacag atccttttgt tgatcccgga
     5461 ttagggaaaa atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca
     5521 gtagcagggt ttgtctctat ggttccttat atgatgaaag atgttcacca gctaagtact
     5581 gccgaaatcg gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac
     5641 attggtggga tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca
     5701 tttctttctg ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg
     5761 acaattataa tcgtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca
     5821 attgtttcaa gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt
     5881 accagctttt tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc
     5941 ttacttgatc aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat
     6001 ttgttattac ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat
     6061 aaacattctc aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa
     6121 ttgatccttt ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc
     6181 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt
     6241 tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa
     6301 gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt
     6361 gatcttctca aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa
     6421 cccccctttg ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa
     6481 agaaaggtct taaaggtttt atggttttgg tcggcactgc cgacagcctc gcagagcaca
     6541 cactttatga atataaagta tagtgtgtta tactttactt ggaagtggtt gccggaaaga
     6601 gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga tt
//
LOCUS       DAZKCN010000273         4673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.145, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000273 DAZKCN010000000
VERSION     DAZKCN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.145"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..209
                     /locus_tag="ACG8K3_002849"
                     /pseudo
     CDS             1..209
                     /locus_tag="ACG8K3_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            447..683
                     /locus_tag="ACG8K3_002850"
     CDS             447..683
                     /locus_tag="ACG8K3_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146616.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="ACG8K3_002851"
     CDS             690..2642
                     /locus_tag="ACG8K3_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP9146617.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8K3_002852"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8K3_002852"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP9146618.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3486..4613
                     /locus_tag="ACG8K3_002853"
                     /pseudo
     CDS             3486..4613
                     /locus_tag="ACG8K3_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
BASE COUNT         1630 a          752 c          837 g         1454 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taa
//
LOCUS       DAZKCN010000274         3970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000274 DAZKCN010000000
VERSION     DAZKCN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.152"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002854"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9146619.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002855"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFP9146620.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1254 a          613 c          818 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAZKCN010000275         3970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.153, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000275 DAZKCN010000000
VERSION     DAZKCN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.153"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002856"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9146621.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002857"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8K3_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFP9146622.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1253 a          613 c          819 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAZKCN010000276         2343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000276 DAZKCN010000000
VERSION     DAZKCN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.172"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG8K3_002858"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG8K3_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG8K3_002859"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG8K3_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACG8K3_002860"
     CDS             complement(1055..1345)
                     /locus_tag="ACG8K3_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9146623.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2245)
                     /locus_tag="ACG8K3_002861"
     CDS             complement(1553..2245)
                     /locus_tag="ACG8K3_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9146624.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          674 a          440 c          337 g          892 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg
     1741 cgatttgttg attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt
     1801 cttgtagtct tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga
     1861 tctcatctag tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga
     1921 ctaattgact ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt
     1981 catttacttt tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt
     2041 tttgtgctag tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat
     2101 caagctgtgt cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa
     2161 tttcatttgc gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc
     2221 caccagtaat aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct
     2281 aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa attacatatg taattttaat
     2341 ata
//
LOCUS       DAZKCN010000277         2001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.180, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000277 DAZKCN010000000
VERSION     DAZKCN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.180"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002862"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9146625.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          688 a          310 c          411 g          592 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta a
//
LOCUS       DAZKCN010000278         2001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000278 DAZKCN010000000
VERSION     DAZKCN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.181"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002863"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8K3_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFP9146626.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          687 a          310 c          412 g          592 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta a
//
LOCUS       DAZKCN010000279         1998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000279 DAZKCN010000000
VERSION     DAZKCN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.182"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..439
                     /locus_tag="ACG8K3_002864"
     CDS             47..439
                     /locus_tag="ACG8K3_002864"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9146627.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="ACG8K3_002865"
     CDS             440..1879
                     /locus_tag="ACG8K3_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFP9146628.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          832 a          153 c          342 g          671 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tgatggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1981 aaaatgctat acaatgtt
//
LOCUS       DAZKCN010000280          828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.227, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000280 DAZKCN010000000
VERSION     DAZKCN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.227"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            215..763
                     /gene="aac(6')"
                     /locus_tag="ACG8K3_002866"
     CDS             215..763
                     /gene="aac(6')"
                     /locus_tag="ACG8K3_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP9146629.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          294 a          124 c          178 g          232 t
ORIGIN      
        1 tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt
       61 tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat
      121 agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga
      181 ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa
      241 taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata
      301 tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc
      361 agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac
      421 aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac
      481 tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt
      541 aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac
      601 atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa
      661 attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat
      721 ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac
      781 agaagtaatc agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAZKCN010000281          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4228
            SAMEA115707057-rid22574403.guided.245, whole genome shotgun
            sequence.
ACCESSION   DAZKCN010000281 DAZKCN010000000
VERSION     DAZKCN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707057
            Sequence Read Archive: ERR13196789
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287864.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287864.1
            Genome Coverage       :: 160x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 08:52:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,867
            CDSs (total)                      :: 2,804
            Genes (coding)                    :: 2,713
            CDSs (with protein)               :: 2,713
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 44 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 27 of 91
            Pseudo Genes (multiple problems)  :: 17 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707057-rid22574403.guided.245"
                     /isolate="VRE4228"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..422
                     /locus_tag="ACG8K3_002867"
     CDS             <1..422
                     /locus_tag="ACG8K3_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFP9146630.1"
                     /translation="ISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTE
                     VDISILSKIPREYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLF
                     SKNQFYQALKYARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          205 a           79 c           69 g          173 t
ORIGIN      
        1 atataagtac agatataatt cgaaaaattg tttttgaaag cacagctaca gaaagttatt
       61 taatagtgga atatggattt gctaaaaggt tgctaaatac aaatcgttca ctagcactat
      121 ttttaatgac agaagttgat atatccatat taagtaaaat ccctagagaa tactttcatc
      181 caaaacctag agttaatagc tcgttaattg tattaaaaag acacccttca aaaatatcac
      241 tcaaagatag aaaacaatat gaaaattttg ttatgaaatg ggttaacaaa gaatacataa
      301 aactattttc caaaaaccaa ttttatcaag ccttaaaata tgcaagaatt gacgatttaa
      361 acaatattag ctttgaacaa ttcttgtctc ttttcaatag ctataaatta tttaatagat
      421 aagttaaggg gtgcataaac tgcatccctt aacttatctt attttgctta aacaatcttg
      481 gcccgtaaac tgcataactc atatgtaaat cgctcctttt taggtg
//
